KR20210100069A - Composition for improvementing, preventing or treating obesity and metabolic diseases comprising fractions or extract of pepper leave - Google Patents

Abstract

The present invention relates to a composition for improving, preventing, or treating obesity or a metabolic disease capable of inhibiting pre-fat cell differentiation and fat accumulation by containing an extract of pepper leave as an effective component; reducing growth of a weight and body fat; reducing concentrations of endotoxin, triglycerides, and total cholesterol in serum; and inhibiting expression of lipid differentiation-related hormones in the serum. The composition according to the present invention is provided to improve not only the obesity but also immune activity, thereby being very useful by treating the obesity and improving, preventing, or treating the metabolic disease induced by the obesity.

Description

A composition for improving, preventing or treating obesity and metabolic diseases comprising a red pepper leaf extract TECHNICAL FIELD

The present invention relates to a composition for improving, preventing or treating obesity and metabolic diseases, containing red pepper leaf extract as an active ingredient, inhibiting pre-adipocyte differentiation and fat accumulation, reducing the increase in body weight and body fat, and endotoxin in serum , lowering the concentration of triglycerides and total cholesterol, and suppressing the expression of hormones related to fat differentiation in the serum, so that it can be usefully used in functional foods and drugs for the improvement, prevention and treatment of obesity and metabolic diseases.

Obesity can be defined as a kind of disease that threatens health due to excessive accumulation of body fat. According to the report of the International Obesity Task Force (IOTF), about 1.7 billion people, who make up a quarter of the world's population, need to lose weight, which is emerging as a serious social problem for both developed and developing countries. there is

Obesity is responsible for 30 to 60% of the rapidly increasing number of lifestyle-related diseases (adult diseases), and according to Japanese statistics, it is analyzed that 2 trillion yen (25 trillion KRW) is required for medical expenses to lose 5 kg of body weight, and the prevalence of overweight and obesity is 1 person per 4 to 5 people, and the increase is expected to continue in the future. This trend is a more serious national problem in the United States and Europe. In Korea, the prevalence of childhood obesity is rapidly increasing due to the westernization of eating habits, and the opportunity to eat high-fat, high-calorie meals has increased due to a rich lifestyle, but it consumes more energy than consumed due to lack of exercise. Because the energy required to do so is remarkably small, the number of obese people is increasing rapidly. In addition, young women are working very hard to maintain the same body shape as when they were young, for beauty effects, and many women after marriage are also overweight or obese.

Dexflenfluramine, Phenylalanine, Tyrosine and Methionine as an appetite suppressant as an anti-obesity material, Orlistat as a fat binding agent (absorption inhibitor), Chitosan, fat burning Zero Silbutramine, Ephedrine, Caffeine and Oilseed, Garcinia Cambogia Fruit, L-Carnitine, Dietary Fiber that Regulates Long-Term Functions: Chacheonjap, Glucomannan Kaktomannan, Chromium Pea, which stabilizes blood sugar Cholic acid (Chromiumpicolinate) and the like. However, although these materials are somewhat recognized for their medicinal efficacy, they have side effects on the heart, blood pressure, and nervous system due to temporary weight loss, rapid weight loss, and drugs.

Pepper ( Capsicum annuum ) is a tropical plant classified as an annual plant belonging to the Solanaceae family , and is one of the most consumed vegetables worldwide. In Korea, it is mainly cultivated from late spring to summer, and has been used as a food ingredient for a long time as the main ingredient of kimchi. Currently, there are about 20-30 kinds of peppers known worldwide, and among them, Capsicum annuum, Capsicum frutescens, Capsicum chinense, Capsicum baccatum, and Capsicum pubescens are the most cultivated. It is reported that red pepper not only contains abundant vitamins and minerals, but also contains a large amount of phenolic compounds such as carotenoid, quercetin, luteolin and capsaicinoids.

The physiological activity of red pepper is known to exhibit various activities such as antioxidant, lipid metabolism, infection, stamina, analgesic, gastric acid secretion, and anti-stress (Oh, 2004; Iwai, 2002). ). However, although a lot of research on pepper is known, research on pepper leaves is insufficient. And although the use of red pepper in daily life is high, the usage of the pepper leaves is low except to the extent of eating vegetables. In particular, there is no known at all about a composition that can effectively improve the improvement, prevention or treatment of obesity using red pepper leaves.

Therefore, the present inventors made diligent efforts to develop a new composition that can effectively improve obesity or metabolic disease from natural products, and as a result, the present inventors found that red pepper leaf extract is stable to the human body and has no side effects, and is a conventional anti-obesity substance or metabolic disease. By confirming that it has the same or higher efficacy as the therapeutic material, the present invention has been completed.

Republic of Korea Patent Publication No. 10-2016-0059152

It is an object of the present invention to provide a food composition for improving or preventing obesity or metabolic disease using a red pepper leaf extract as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of obesity or metabolic disease comprising a red pepper leaf extract as an active ingredient.

In order to achieve the above object, the present invention provides a food composition for improving or preventing obesity or metabolic disease, comprising a red pepper leaf extract as an active ingredient.

The red pepper leaf extract may be extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The composition inhibits pre-adipocyte differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers serum endotoxin, triglyceride, and total cholesterol concentrations, and suppresses the expression of hormones related to adipogenesis in serum. .

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating obesity or metabolic disease comprising a red pepper leaf extract as an active ingredient.

The red pepper leaf extract may be extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The composition inhibits pre-adipocyte differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers serum endotoxin, triglyceride, and total cholesterol concentrations, and suppresses the expression of hormones related to adipogenesis in serum. .

The composition of the present invention comprises a red pepper leaf extract, and the composition inhibits pre-adipocyte differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers serum endotoxin, triglyceride and total cholesterol concentrations, and reduces serum It has the effect of suppressing the expression of hormones related to fat differentiation. In addition, the composition according to the present invention can prevent, improve, or treat metabolic diseases as well as obesity by enhancing immune activity as well as obesity. there is.

1 is an Oil red O staining result for confirming the change in intracellular lipid content of 3T3-L1 by treatment with a hot pepper leaf hot water extract prepared in Example 1 and a red pepper leaf polymer fraction prepared in Example 2. FIG.
2 is a result of experiments on the inhibitory effect on adipocyte differentiation of the hot pepper leaf extract prepared in Example 1 and the red pepper leaf polymer fraction prepared in Example 2;
3 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of garcinia cambogia extract of Comparative Example 1 (GC), a 50 mg/kg group administered with a red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the change in body weight over time in the 100 mg/kg administration group (WEPL100) of the red pepper leaf extract of Example 1.
4 shows a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And after extracting the white epididymal adipose tissue from the 100 mg/kg red pepper leaf extract of Example 1 administered group (WEPL100), it is a graph showing by measuring the weight.
5 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the endotoxin concentration in the serum extracted from the 100 mg / kg red pepper leaf extract of Example 1 administration group (WEPL100) was measured.
6 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) and a graph showing the concentration of triglycerides in the serum extracted from the 100 mg/kg red pepper leaf extract of Example 1 (WEPL100).
7 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the total cholesterol concentration in the serum extracted from the 100 mg / kg red pepper leaf extract of Example 1 administered group (WEPL100).
8 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the measurement of the leptin concentration in the serum extracted from the 100 mg / kg red pepper leaf extract of Example 1 administration group (WEPL100).
9 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the measurement of the insulin concentration in the serum extracted from the 100 mg / kg red pepper leaf extract of Example 1 (WEPL100) administration group (WEPL100).

The present invention relates to a composition for improving, preventing or treating obesity or metabolic disease, comprising a red pepper leaf extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for improving, preventing or treating obesity or metabolic disease, comprising the red pepper leaf extract as an active ingredient.

Pepper ( Capsicum annuum ) is a tropical plant classified as an annual plant belonging to the Solanaceae family , and is one of the most consumed vegetables worldwide. In Korea, it is mainly cultivated from late spring to summer, and has been used as a food ingredient for a long time as the main ingredient of kimchi. Currently, there are about 20-30 kinds of peppers known worldwide, and among them, Capsicum annuum, Capsicum frutescens, Capsicum chinense, Capsicum baccatum, and Capsicum pubescens are the most cultivated. It is reported that red pepper contains abundant vitamins and minerals as well as phenolic compounds such as carotenoid, quercetin, luteolin and capsaicinoids in large amounts, but studies on pepper leaves are insufficient.

In the present invention, the term 'obesity' refers to a state in which body fat is excessively accumulated, rather than simply being overweight. If you have a high percentage of body fat, even if you appear to be of normal weight, it means that you are obese. Generally, obesity is determined using body mass index (BMI), where 23 to 24.9 is considered overweight, 25 to 29.9 is considered mild obesity, 30 to 34.9 is moderate obesity, and 35 or more is considered severe obesity. are doing Obesity is caused by a combination of several causes rather than a single cause. Including westernized eating habits, wrong eating habits, reduced activity, emotional factors, and genetic factors, obesity that occurs in this way eventually leads to chronic diseases such as hyperlipidemia, diabetes, and high blood pressure. increases the risk of developing the disease.

In the present invention, the term 'metabolic disease' refers to a condition or disease closely related to or caused by obesity, specifically selected from the group consisting of fatty liver, type 2 diabetes, hyperlipidemia, cardiovascular disease, and arteriosclerosis. There may be more than one.

In the present invention, the fatty liver (fatty liver) refers to a condition or disease in which fat is accumulated in hepatocytes in an excessive amount due to a fat metabolism disorder of the liver.

In the present invention, hyperlipidemia refers to a condition or disease in which the concentration of fat components in the blood, particularly cholesterol and triglyceride, is higher than normal values, and includes all conditions required to lower the lipid concentration in the blood. used in a broad sense.

As used herein, arteriosclerosis refers to a condition or disease in which blood circulation to organs and tissues in the body is reduced due to the thickening of the arterial wall and decreased elasticity, and other substances such as fat and cholesterol are deposited on the inner wall of the artery to form plaque ( It has a meaning including "atherosclerosis", which means a condition or disease in which blood circulation is reduced by forming a plaque) and narrowing the lumen. Atherosclerosis can occur anywhere in the body, and when it occurs in blood vessels in the heart, it causes coronary artery disease such as angina pectoris and myocardial infarction, it can cause cerebral infarction that occurs in the brain, and when it occurs in the kidney, it can cause kidney failure. can be induced

Specifically, the composition for improving, preventing, or treating obesity or metabolic disease may be referred to as a composition for improving, preventing, alleviating, suppressing and treating weight or body fat increase or metabolic disease caused by the various causes. More preferably, it has an effect of improving, preventing, and treating obesity or metabolic disease induced by a high-fat diet.

According to the experimental results of the present invention, it can be seen that body weight and body fat increased when a high-fat diet was administered for a long time. This induces differentiation of adipocytes and fat accumulation over time, increases body weight and body fat content, increases serum endotoxin, triglyceride and total cholesterol concentrations as well as expression of fat differentiation-related hormones, resulting in obesity or metabolism. lead to disease.

At this time, co-administration of red pepper leaf extract inhibits pre-adipocyte differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers serum endotoxin, triglyceride, and total cholesterol levels, and reduces the expression of hormones related to fat differentiation in serum. inhibition can be observed. Therefore, it can be seen that the composition comprising the red pepper leaf extract according to the present invention as an active ingredient has an effect of improving, preventing or treating obesity or metabolic disease.

In the present invention, 'extract' includes not only the crude extract obtained by treating the extraction raw material with an extraction solvent, but also the processed product of the crude extract. For example, the red pepper leaf extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

Also, in the present invention, the term 'extract' includes a fraction obtained by additionally fractionating the crude extract. That is, the red pepper leaf extract includes not only those obtained using an extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a polymer fraction obtained by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. method can be purified.

Preferably, the polymer fraction may be a fraction obtained by obtaining a red pepper leaf extract or a red pepper leaf extract using an extraction solvent, and then separating only a high molecular weight material having a molecular weight of 10 kDa or more.

In addition, in the present invention, the 'red pepper leaf extract' is an extract of the red pepper leaf with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and the extraction method does not need to be particularly limited. Preferably, the red pepper leaf extract may be a hot pepper leaf extract or a red pepper leaf ethanol aqueous solution, for example, a red pepper leaf alcohol extract. Most preferably, it may be a hot water extract of red pepper leaves.

In addition, the red pepper leaf extract may be processed by processing the red pepper leaf extract, and repeatedly extracted one or more times with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The red pepper leaves may be cut to a certain size and dried using a shade or a dryer, then crushed or used as it is. The drying period is not particularly limited as long as the moisture content in the red pepper leaves is less than 20%.

Compared to other parts of red pepper, the use of red pepper leaves inhibited pre-adipocyte differentiation and fat accumulation, reduced the increase in body weight and body fat, and lowered serum endotoxin, triglyceride and total cholesterol levels when a high-fat diet was administered for a long period of time. Since it is 1.5 to 2.5 times better in suppressing the expression of hormones related to fat differentiation in serum, the red pepper leaf extract is significantly more effective in preventing, improving, or treating obesity or metabolic disease than other parts of red pepper.

In the present invention, 'consisting of an active ingredient' or 'comprising it as an active ingredient' means including an amount sufficient to improve, prevent or treat obesity or metabolic disease of the red pepper leaf extract of the present invention. The red pepper leaf extract is a natural product, and since there is no side effect to the human body even when administered in excess, the quantitative upper and lower limits of the red pepper leaf extract contained in the composition of the present invention can be selected and carried out by those skilled in the art within an appropriate range.

The composition of the present invention is a food composition, and can be used in various ways as a food composition for improving or preventing obesity or metabolic disease using the red pepper leaf extract of the present invention as an active ingredient. It can be formulated in the same manner as the following pharmaceutical composition comprising the red pepper leaf extract of the present invention as an active ingredient and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, sweets, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice cream, vitamin complexes, health supplements etc.

The food composition of the present invention may include not only red pepper leaf extract as an active ingredient, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink or beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be additionally included in addition to the red pepper leaf extract of the present invention.

The present invention provides a health functional food comprising a food composition for improving, inhibiting or preventing obesity or metabolic disease comprising the red pepper leaf extract as an active ingredient. Health functional food refers to food prepared by adding red pepper leaf extract to food ingredients such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspension, etc. However, unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long period of time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The amount of red pepper leaf extract added in such a health functional food varies depending on the type of health functional food and cannot be uniformly defined, but it can be added within a range that does not impair the original taste of the food, and is usually 0.01 for the target food. to 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In another example, as described above, it can be used as a pharmaceutical composition for preventing or treating obesity or metabolic disease using the red pepper leaf extract as an active ingredient. It can be prepared using pharmaceutically suitable and physiologically acceptable adjuvants in addition to the red pepper leaf extract as the active ingredient, and the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, lubricants or flavoring agents. etc. can be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation in the form of a tablet or capsule, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.

Furthermore, by using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the art, it can be preferably formulated according to each disease or component.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration mode, age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and response sensitivity of the patient, usually Thus, a skilled physician can easily determine and prescribe an effective dosage for the desired treatment or prevention. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g/kg, and more preferably, it may be administered in a dose of 0.1 g/kg to 10 g/kg.

The pharmaceutical composition of the present invention may be prepared in a unit dose form by formulation using a pharmaceutically acceptable carrier and/or excipient, or may be prepared by internalizing in a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion in oil or an aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or stabilizer.

In addition, the present invention provides a method for improving, preventing or treating obesity or metabolic disease, comprising administering an effective amount of a red pepper leaf extract to a mammal.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term “effective amount” refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as conceived by a researcher, veterinarian, physician or other clinician, which is in an amount that induces relief of The effective amount and frequency of administration for the active ingredient of the present invention may vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and body weight, body fat, serum triglyceride and cholesterol or obesity or metabolic disease, content of active ingredients and other ingredients contained in the composition, type of formulation, and the patient's age, weight, general health status, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and concurrently used drugs. In the prevention, treatment or improvement method of the present invention, in the case of adults, when the red pepper leaf extract is administered once to several times a day, it is preferably administered at a dose of 0.001 g/kg to 10 g/kg, more preferably It may be administered in a dose of 0.1 g/kg to 10 g/kg.

In the treatment method of the present invention, the composition comprising red pepper leaf extract as an active ingredient is administered in a conventional manner through oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. It can be administered, and preferably administered orally or intraperitoneally.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1. Red pepper leaf hot water extract

Domestic dried red pepper leaves produced in Yecheon Nonghyup, Gyeongbuk were purchased and used. Red pepper leaves were dried in a drying oven at 50° C. for 24 hours, and then pulverized to a size of 1.0 mm or less with a grinder to prepare red pepper leaf powder.

Distilled water corresponding to 20 times the volume was added to 5 g of red pepper leaf powder and extracted under reflux at 80° C. for 3 hours to obtain a hot water extract. After the hot water extract was filtered, it was concentrated under reduced pressure using a rotary vacuum evaporator and lyophilized for 72 hours. The hot water extract of red pepper leaves obtained by the above method was described as WEPL (Water-soluble extract from pepper leaves).

Example 2. Red pepper leaf polymer fraction

Domestic dried red pepper leaves produced in Yecheon Nonghyup, Gyeongbuk were purchased and used. Red pepper leaves were dried in a drying oven at 50° C. for 24 hours, and then pulverized to a size of 1.0 mm or less with a grinder to prepare red pepper leaf powder.

Distilled water corresponding to 20 times the volume was added to 100 g of red pepper leaf powder and extracted under reflux at a temperature of 80° C. for 3 hours to obtain a hot water extract. After filtering the hot water extract, it was concentrated under reduced pressure to 1/10 of the extract using a rotary vacuum evaporator, and 80% alcohol corresponding to 4 times the volume (v/v) of the hot water extract concentrated under reduced pressure. was added to the hot water extract and precipitated for 24 hours. This was centrifuged (6000 rpm, 635 × g, 30 minutes) to obtain a precipitate, and a small amount of distilled water was added thereto to re-dissolve the precipitate, and a dialysis membrane (MW cut off 12,000, Sigma-Aldrich Co., St. Louis, MO, USA) for 2-3 days to remove low molecular weight substances and freeze-dried for 72 hours to obtain a red pepper leaf polymer fraction. The red pepper leaf polymer fraction obtained by the above method was named HFPL (High-molecular fraction from pepper leaves).

Comparative Example 1. Garcinia cambogia extract

Garcinia cambogia extract was purchased from Unicon Natural Products PVT in India and used. At this time, the Garcinia cambogia extract was used to contain HCA in an amount of 600 mg/g or more.

<Test Example>

Test Example 1. Analysis of yield, sugar and protein content of the hot pepper leaf hot water extract of Example 1 and the red pepper leaf polymer fraction of Example 2

Neutral sugar analysis was performed by partially modifying the method of Albersheim et al., and derivatizing each constituent sugar with alditol acetate and aldonolactone after hydrolysis to GC (Gas Chromatography ACME-6100, Young-Lin Co. Ltd. Anyang, Korea). ) was used for analysis. The sample of Example 1 or Example 2 was hydrolyzed by reacting it at 121° C. for 1.5 hr in 2 M TFA (trifluroacetic acid), and then dissolved in 1 ml of 1 M NH ₄ OH (ammonia solution) to dissolve 10 mg of NaBH ₄ was reduced for 4 hr. Acetic acid was added in an appropriate amount _{to remove residual NaBH 4 ,} and then, methanol was added and repeated drying was performed to remove excess acetic acid and converted to alditol. 1 ml of acetic anhydride was added to the alditol, and the mixture was reacted at 121° C. for 30 min to convert to alditol acetate, which was _{separated and extracted with chloroform/H 2} O two-phase solvent system. After drying, the extract was dissolved in a small amount of acetone and used as a sample for GC analysis.

Protein quantification was measured according to the Bradford method, and using BSA (Bovine Serum Albumin, sigma aldrich) as a standard, 1 mg/ml was diluted to a constant multiple to the highest concentration. After reacting by adding 20 µl of each standard and sample to 980 µl of Bradford's reagent, absorbance was measured at 595 nm using a microplate reader. Protein quantification was converted to the amount for BSA.

Total sugar was measured according to the phenol-sulfuric acid method, and the same amount of 5% phenol solution was added to 0.5 mL of the sample solution and stirred. Then, 2.5 mL of concentrated sulfuric acid (98%, v/v) was added and reacted at room temperature for 20 minutes. Absorbance was measured at 470 nm using a reader. Quantification of total sugar was converted to the amount by creating a calibration curve using glucose standard (sigma aldrich).

Total polyphenol content was analyzed according to Folin Denis method using gallic acid (sigma aldrich).

In the case of constituent sugars, a high-performance anion-exchange chromatography-pulsed amperometric detector (HPAEC-PAD) was used after hydrolysis of the sugar chains of the sample into simple sugars, and the data were analyzed using Chromelon 6.80 software (Dionex). Monosaccharides (fucose, rhamnose, arabinose, galactose, glucose, xylose) were used as standard solutions.

As a result, the results of analysis of the physicochemical properties such as the content of major components of the hot water extract and the polymer fraction from red pepper leaves are shown in Table 1 below.

division Example 1 (WEPL) Example 2 (HFPL) Extraction yield(%) 12.66 2.68 Chemical composition (%) Protein 4.5 ± 0.2 4.3 ± 0.4 Neutral sugar 70.7 ± 2.2 52.4 ± 0.5 Uronic acid 16.9 ± 0.1 36.7 ± 1.1 KDO-like material 0.1 ± 0.1 2.5 ± 0.5 polyphenol 7.8 ± 0.4 4.0 ± 0.7 Component sugar (PMP) (Mole %) Rhamnose 9.3 ± 0.1 3.6 ± 0.1 Fucose - 0.2 ± 0.0 Arabinose 2.5 ± 0.2 4.9 ± 0.2 Xylose 0.6 ± 0.1 0.6 ± 0.1 Mannose 0.8 ± 0.0 0.8 ± 0.0 Galactose 8.5 ± 0.1 7.1 ± 0.1 Glucose 54.8 ± 0.5 34.6 ± 0.6 Glucuronic acid - 1.5 ± 0.0 Galacturonic acid 11.1 ± 0.8 36.0 ± 1.1

* The content ratio (%) of the chemical component indicates the ratio in the dry sample.

As shown in Table 1, the hot water extract of red pepper leaf prepared in Example 1 showed a yield of 12.66%, a protein content of 4.5 wt%, and a neutral sugar content of 70.7 wt%, indicating a high sugar content. In addition, as a result of analyzing the constituent sugars of the hot pepper leaf extract prepared in Example 1, the main constituent sugars were glucose (54.8 Mole %), and galacturonic acid, rhamnose, and galactose were contained at 11.1, 9.3, and 8.5 Mole %, respectively. , it was confirmed that arabionose, mannose, and xylose were present in trace amounts at 2.5, 0.8, and 0.6 Mole %, respectively.

In contrast, the yield of the red pepper leaf polymer fraction prepared in Example 2 was as low as 2.68%, but it was confirmed that the protein content was 4.3% by weight, the neutral sugar content was 52.4% by weight, and the uronic acid was 36.7% by weight. In addition, in the red pepper leaf polymer fraction prepared in Example 2, galacturonic acid and glucose were detected as 36.0 and 34.6 Mole %, respectively, and galactose, arabinose, rhamnose, glucuronic acid, mannose, xylose, and fucose were 7.1 and 4.9, respectively. 3.6, 1.5, 0.8, 0.6, 0.2 Mole % was found to be present in trace amounts. In the red pepper leaf polymer fraction (HFPL) of Example 2, the sugar content was overall decreased compared to the hot pepper leaf hot water extract of Example 1, but it was confirmed that the contents of Arabinose, Fucose, and Glucuronic acid slightly increased. That is, it can be seen that there is a clear difference in the specific components present in the two extracts.

Test Example 2. Efficacy analysis of pre-adipocyte differentiation inhibition and fat accumulation inhibition

It was attempted to confirm the anti-obesity effect from the hot pepper leaf hot water extract of Example 1 and the red pepper leaf polymer fraction of Example 2.

1) cell culture

For the experiment, 3T3-L1 pre-adipocytes were purchased from the American Type Culture Collection (ATCC), 10% fetal bovine serum (FBS), 1% penicillin and strptomycin (Gibco BRL, Grand Island, NY, USA) containing DMEM (Dulbecco's Modified EaDCRT Media (Gibco BRL)) was used and cultured at _{37 °C, 5% CO 2 conditions.} In order to solve the over-density phenomenon caused by the proliferation of the number of cells, the growth medium was exchanged every 48 hours to maintain an appropriate number of cells.

(2) differentiation induction

3T3-L1 cells were seeded in a ^{6-well plate at a density of 0.8×10 5} cells/well, and subcultured using 0.05% Trypsin-EDTA every 48 hours. To induce differentiation into adipocytes, MDI (0.5 mM 3-isobutyl-1-methylxanthin, IBMX), 1 μM dexamethasone, 10 μg/mL insulin ) and 10% by volume of fetal bovine serum (FBS) were cultured in DMEM differentiation medium for 48 hours. Thereafter, culture was continued until the point of differentiation was completed while solidifying every 2 days with a medium (DMEM) containing only 10 μg/mL insulin. In order to confirm the effect of the hot pepper leaf extract and the red pepper leaf polymer fraction in the process of adipogenesis, 50 μg/ml (WEPL-50) and 100 μg/ml (WEPL-100) of the hot pepper leaf extract of Example 1 whenever the medium is exchanged , 50 μg/ml (HFPL-50) and 100 μg/ml (HFPL-100) of the red pepper leaf polymer fraction of Example 2 and 50 μg/ml (GC-50) and 100 μg/ml of the Garnicia cambogia extract of Comparative Example 1 (GC-100) was treated together.

When the medium was exchanged, the control group (C) treated with MDI (0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 1 μM dexamethasone, 10 μg/mL insulin), which is a lipid accumulation inducing factor, not an extract. ), treated with only a medium (DMEM) containing only 10 μg/mL insulin without any treatment was considered a normal group (NC).

(3) Oil Red O staining and quantification

On the 8th day after adipocyte differentiation was completed, each cell was recovered, and the adipogenesis inhibitory activity was evaluated therefrom as follows. To quantitatively analyze the fat accumulation of 3T3-L1 cells differentiated into adipocytes through Oil Red O staining, they were washed with PBS, fixed with 3.7% formalin for 10 minutes, and washed with 60% isopropanol. Then, each well was completely dried. After treatment with oil red O dye at room temperature for 20 minutes, and washing with distilled water 4 times, the number and size of lipid droplets of 3T3-L1 adipocytes differentiated in each group were measured under a microscope (Eclipse ti, Nikon). observed through. For quantitative analysis, after extracting fat using 100% isopropanol, 200 μl of each was transferred to a 96-well plate, absorbance was measured at 500 nm with an ELISA reader, and expressed as a percentage of the absorbance value of the control group.

(4) Effect of red pepper leaf extract on adipocyte production of 3T3-L1 preadipocytes

It was investigated whether the formation of lipid droplets appearing in the differentiation of 3T3-L1 preadipocytes into adipocytes was inhibited by treatment with hot pepper leaf extract or red pepper leaf polymer fraction.

1 is an Oil red O staining result for confirming the change in intracellular lipid content of 3T3-L1 by treatment with a hot pepper leaf hot water extract prepared in Example 1 and a red pepper leaf polymer fraction prepared in Example 2. FIG. 2 is a result of experiments on the inhibitory effect on adipocyte differentiation of the hot pepper leaf extract prepared in Example 1 and the red pepper leaf polymer fraction prepared in Example 2;

As shown in FIGS. 1 and 2 , it can be seen that both the WEPL group or the HFPL group inhibited liposynthesis than the control group (C) induced to accumulate lipids. In particular, in the WEPL-100 group, the efficacy of inhibiting adipocyte differentiation and adipogenesis was the most outstanding.

According to FIG. 2, the GC-50 group treated with the Garcinia cambogia extract (Comparative Example 1) showed about 10.3% fat accumulation inhibitory power compared to the control group (C) induced to accumulate lipids, and the WEPL-50 group and WEPL- Group 100 showed 1.9% and 7.6% fat accumulation inhibition, respectively. In addition, the HFPL-50 group and the HFPL-100 group showed 3.0% and 13.7% fat accumulation inhibition, respectively. In summary, the red pepper leaf extract as well as the red pepper leaf polymer fraction according to the present invention exhibited the effect of inhibiting the differentiation of 3T3-L1 preadipocytes into adipocytes and adipogenesis, which was confirmed to be equivalent to that of the conventional Garcinia cambogia extract. Garcinia cambogia extract is known to cause side effects in the human body, so there is a limitation in practical use, but the red pepper leaf extract or red pepper leaf polymer fraction of the present invention has the advantage that there is no limitation in utilization because it hardly shows toxicity in vivo.

Test Example 3. Analysis of factors related to obesity and metabolic diseases of hot pepper leaf extract of Example 1

(1) Experimental animals and diet

45 6-week-old male C57BL/6J mice bred in an aseptic environment were purchased from Central Laboratory Animals Co., Ltd., and the mouse breeding conditions were maintained at a temperature of 22 ± 2 °C, humidity at 40-60%, and light-dark cycle ( Light and dark cycle) was controlled at 12 hour intervals.

The mice were divided into 5 groups of 9 mice each, fed an experimental diet and bred for 8 weeks. The experimental diet was a high fat diet, and the Modified AIN 76A purified rodent diet (60 cal% fat) in which the calorific value was increased by 60 cal% by adding Lard was freely fed, and the normal group (NC), the high fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg of red pepper leaf extract of Example 1 (WEPL50), and a group administered with 100 mg/kg of red pepper leaf extract of Example 1 (WEPL100) was divided into experimental groups. Water and experimental diet were allowed to be freely ingested, and the amount of weight gain during the experiment was measured at 10 am at intervals of 1 week for 8 weeks from the day the experimental diet was started. Garcinia cambogia extract of Comparative Example 1 and red pepper leaf extract of Example 1 were each dissolved in sterile saline and orally administered.

(2) Collection of blood and organs

At the end of the experiment, the experimental animals were fasted for 12 hours, anesthetized using Ifraan solution, and the blood collected from the portal vein was coagulated by incision in the abdomen and thoracic cavity. was used for analysis. White epididymal adipose tissue and other organs were removed from each experimental animal, blood and foreign substances were removed using physiological saline, the weight was measured, and stored at -80°C until analysis.

(3) Weight change and body fat mass analysis

3 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of garcinia cambogia extract of Comparative Example 1 (GC), a 50 mg/kg group administered with a red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the change in body weight over time in the 100 mg/kg red pepper leaf extract administered group (WEPL100) of Example 1, Figure 4 is a normal group (NC), a high fat diet control group (HF), the garcinia cambogia extract of Comparative Example 1 50 After extracting the white epididymal adipose tissue from the positive control group (GC) administered at mg/kg, the group administered with 50 mg/kg of red pepper leaf extract of Example 1 (WEPL50), and the group administered with 100 mg/kg of the red pepper leaf extract of Example 1 (WEPL100), It is a graph showing by measuring the weight.

As shown in FIG. 3 , compared to the normal group (NC), the high fat diet control group (HF) showed a significantly higher increase in body weight (11.9 g increase). On the other hand, the group to which the Garcinia cambogia extract of Comparative Example 1 and the red pepper leaf extract of Example 1 were orally administered showed a tendency to significantly decrease body weight compared to the high-fat control group (HF). Specifically, the group (GC) administered orally administered 50 mg/kg of Garnicia cambogia extract (Comparative Example 1) while on a high-fat diet lost 26.9% of body weight compared to the high-fat control group (HF), and while eating a high-fat diet, 50 mg/kg of red pepper leaf extract The mg/kg administration group (WEPL50) and the 100 mg/kg administration group (WEPL100) reduced body weight by 25.1% and 28.5%, respectively, compared to the high fat diet control group (HF). In particular, the WEPL-100 group, to which 100 mg/kg of the red pepper leaf extract of Example 1 was orally administered, significantly decreased body weight compared to the positive control group (GC group) (p<0.05).

As shown in FIG. 4 , the amount of white epididymal fat extracted from each experimental group was significantly increased in the high-fat control group (HF) compared to the normal group (NV). It was confirmed that the weight of white epididymal adipose tissue was reduced by about 2.7% and 16.1% in the WEPL-50 group and the WEPL-100 group administered with the red pepper leaf extract of Example 1 compared to the high-fat diet control group (HF). Therefore, it can be seen that the red pepper leaf extract of the present invention has an effect of reducing the weight of adipose tissue, and suppresses an increase in body fat as well as a weight loss.

(4) Analysis of plasma lipid content

The concentrations of endotoxin, triglycerides, and total cholesterol in serum were analyzed by enzymatic method using a blood analysis kit of Shinyang Chemical Co., Ltd. Triglycerides were analyzed with Tiglyzyme-V and total cholesterol was analyzed with Cholestezyme-V. The amount of Endotoxin in mouse serum was confirmed using Pierce™ LAL Chromogenic Endotoxin Quantitation Kit (Thermo scientific).

5 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the endotoxin concentration in the serum extracted from the 100 mg/kg red pepper leaf extract administration group (WEPL100) of Example 1, and FIG. 6 is a normal group (NC), a high-fat control group (HF), garcinia of Comparative Example 1 Neutrality in serum extracted from the positive control group (GC) administered 50 mg/kg of cambogia extract, 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) and 100 mg/kg red pepper leaf extract of Example 1 (WEPL100) It is a graph showing the measurement of the fat concentration, and FIG. 7 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of the Garcinia cambogia extract of Comparative Example 1 (GC), and red pepper leaf of Example 1 It is a graph showing the total cholesterol concentration in serum extracted from the extract 50 mg/kg administration group (WEPL50) and the red pepper leaf extract 100 mg/kg administration group of Example 1 (WEPL100).

As shown in Figure 5, endotoxin was increased by 106.3% in the high-fat control group (HF) compared to the normal group (NC), but the WEPL-50 group and the WEPL-100 group were 14.6% and 14.6% each compared to the high-fat control group (HF). Since it was reduced by 32.9%, it was confirmed that the red pepper leaf extract of Example 1 had the effect of reducing the concentration of endotoxin in the serum to suppress obesity and at the same time prevent inflammation induced by obesity.

6 and 7, triglycerides and total cholesterol were significantly increased in the high-fat control group (HF) compared to the normal group (NC), but the WEPL-50 group and the WEPL-100 group were compared to the high-fat diet control group (HF). decreased significantly. The red pepper leaf extract of Example 1 according to the present invention was only administered in combination with a high-fat diet, and exhibited triglyceride and total cholesterol similar to those of the normal group (NC), indicating that the anti-obesity effect was remarkably excellent.

(5) Plasma fat differentiation-related hormones (leptin, insulin)

The concentrations of leptin (mouse leptin ELISA kit, R&D systems, Minneapolis, USA) and insulin (mouse insulin ELISA kit, ALPCO, New Hampshire, USA), which are hormones related to fat differentiation in serum, were analyzed using an ELISA assay.

8 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered 50 mg/kg of Garcinia cambogia extract of Comparative Example 1 (GC), a group administered with 50 mg/kg red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the concentration of leptin in the serum extracted from the 100 mg/kg red pepper leaf extract administered group (WEPL100) of Example 1, and FIG. 9 is a normal group (NC), a high fat diet control group (HF), and garcinia of Comparative Example 1 Insulin in serum extracted from the positive control group (GC) administered with cambogia extract 50 mg/kg, the red pepper leaf extract 50 mg/kg administration group of Example 1 (WEPL50), and the red pepper leaf extract 100 mg/kg administration group of Example 1 (WEPL100) It is a graph showing by measuring the concentration.

As shown in FIGS. 8 and 9 , leptin is a hormone produced and secreted by adipose tissue, and its blood concentration is in proportion to the amount of body fat, and is known to be involved in energy metabolism and weight control by suppressing appetite and increasing energy consumption. Leptin concentration was decreased by 31.8%, 18.2%, and 39.1% in the GC group, WEPL-50 group, and WEPL-100 group, respectively, compared to the high fat diet control group (HF). In addition, as a result of measuring the serum insulin concentration, the GC group, WEPL-50 group, and WEPL-100 group decreased by 32.3%, 18.1%, and 38.9%, respectively, compared to the high-fat control group (HF). Therefore, the red pepper leaf extract of Example 1 showed a significant decrease in serum leptin and insulin concentrations, which is associated with a decrease in body fat mass.

When the red pepper leaf extract of Example 1 is combined with the previous results, it is confirmed that when 50 mg/kg or more is used, it shows an improvement, prevention or treatment effect of obesity, but it is more preferable because it shows the most excellent effect when it is 100 mg/kg or more. , Since there is no increase in effect when used at a concentration of 10 g/kg or more, it is most preferably used at a concentration of 100 to 10,000 mg/kg.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Preparation of powder

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 500 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2. Preparation of tablets

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 300 mg

corn starch 100 mg

lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3. Preparation of capsules

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 200 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 600 mg

mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

Formulation Example 5. Preparation of liquid formulation

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 4 g

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 g by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.

Formulation Example 6. Preparation of granules

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 1,000 mg

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, the mixing ratio may be arbitrarily modified. It can be prepared and used in the production of a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

Red pepper leaf hot water extract obtained in Example 1 or Red pepper leaf polymer fraction obtained in Example 2 1,000 mg

citric acid 1,000 mg

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 ml with purified water

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use.

Claims (6)

A food composition for improving or preventing obesity, comprising a red pepper leaf polymer fraction as an active ingredient. According to claim 1,
The red pepper leaf polymer fraction is a food composition for improving or preventing obesity, characterized in that the red pepper leaf extract is fractionated with a molecular weight of 10 kDa or more. According to claim 1,
A food composition for improving or preventing obesity, characterized in that it is prepared by precipitating a hot pepper leaf hot water extract with alcohol, and passing the precipitate through an ultrafiltration membrane to recover only a polymer fraction having a molecular weight of 10 kDa or more. A pharmaceutical composition for preventing or treating obesity, comprising a red pepper leaf polymer fraction as an active ingredient. According to claim 1,
The red pepper leaf polymer fraction is a pharmaceutical composition for preventing or treating obesity, characterized in that the red pepper leaf extract is fractionated with a molecular weight of 10 kDa or more. According to claim 1,
A pharmaceutical composition for the prevention or treatment of obesity, characterized in that it is prepared by precipitating a hot water extract of red pepper leaf with alcohol and passing the precipitate through an ultrafiltration membrane to recover only a polymer fraction having a molecular weight of 10 kDa or more.

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