KR20200125156A - Composition for improvementing, preventing or treating obesity and metabolic diseases comprising fractions or extract of pepper leave - Google Patents

Abstract

The present invention relates to a composition for mitigating, preventing, or treating obesity and metabolic diseases and, more specifically, to a composition for mitigating, preventing, or treating obesity and metabolic diseases, which includes a pepper leaf extract as an active ingredient to inhibit differentiation of preadipocytes and accumulation of fat, reduce an increase of weight and body fat, lower the concentrations of endotoxin, neutral fat, and total cholesterol in the blood, and inhibit expression of fat differentiation-related hormones in the blood. According to the present invention, the component increases immunity activity as well as anti-obesity activity to treat obesity and also mitigate, prevent, and treat metabolic diseases induced by obesity, thereby being useful.

Description

Composition for improvementing, preventing or treating obesity and metabolic diseases comprising fractions or extract of pepper leave}

The present invention relates to a composition for improving, preventing or treating obesity and metabolic diseases, containing red pepper leaf extract as an active ingredient, inhibiting proliferative cell differentiation and fat accumulation, reducing weight and body fat increase, and endotoxin in serum , It can be usefully used in functional foods and drugs for improvement, prevention and treatment of obesity and metabolic diseases by lowering the concentration of triglycerides and total cholesterol, and inhibiting the expression of hormones related to differentiation in serum.

Obesity can be defined as a type of disease that threatens health due to excessive body fat accumulation. According to a report by the International Obesity Task Force (IOTF), about 1.7 billion people, which account for a quarter of the world's population, need to lose weight, which has emerged as a serious social problem for both developed and developing countries. have

30 to 60% of the rapidly increasing lifestyle-related diseases (adult diseases) are caused by obesity, and according to Japanese statistics, it is analyzed that 2 trillion yen (25 trillion won) is required for medical expenses to lose 5 kg weight, and the incidence of overweight and obesity For every 4-5 people, the number is expected to continue increasing in the future. This trend is a more serious national problem in the United States and Europe, and the incidence of childhood obesity has rapidly increased due to the westernization of dietary habits in Korea, and the chances of eating high-fat, high-calorie meals have increased due to the rich life, but consumed more than energy consumed such as lack of exercise. Because the energy to do is remarkably small, obese people are increasing rapidly. In addition, young women are making great efforts to maintain the same body shape as when they were young because there are many overweight or obese women after marriage.

Anti-obesity materials include dexflenfluramine, phenylalanine, tyrosine and methionine as appetite suppressants, as well as fat binding agents (absorption inhibitors), orlistat, chitosan, and fat burning. Zero silbutramine, ephedrine, caffeine and fat, garcinia cambogia fruit, L-carnitine, dietary fiber that regulates intestinal function: tea puncture, glucomannan keratomannan, chromium blood that stabilizes blood sugar Cholic acid (Chromium picolinate) and the like. However, although these materials are somewhat effective, there are side effects on the heart, blood pressure, and nervous system due to temporary weight loss, rapid weight loss, and drugs.

Pepper ( Capsicum annuum ) is a tropical plant, classified as an annual plant belonging to the Solanaceae family , and is one of the most consumed vegetables worldwide. In Korea, it is cultivated mainly from late spring to summer, and has been used as a food ingredient for a long time as the main ingredient of kimchi. Currently, there are about 20 to 30 varieties of pepper known worldwide, of which Capsicum annuum, Capsicum frutescens, Capsicum chinense, Capsicum baccatum, and Capsicum pubescens are the most cultivated. Peppers are reported to be rich in vitamins and minerals, as well as high amounts of phenolic compounds such as carotenoid, quercetin, luteolin and capsaicinoids.

These physiological activities of pepper are known to exhibit various activities such as antioxidant, lipid metabolism, infection, physical strength, analgesic, gastric acid secretion, and anti-stress (Oh, 2004; Iwai, 2002). ). However, many studies on red pepper are known, but studies on red pepper leaves are insufficient. In addition, the utilization of red pepper in daily life is high, but the utilization of red pepper leaves is low, except for eating herbs. In particular, there is no known composition that can effectively improve the improvement, prevention or treatment of obesity using red pepper leaves.

Therefore, the present inventors have made diligent efforts to develop a new composition that can effectively improve obesity or metabolic diseases from natural products, and as a result, the present inventors have made red pepper leaf extract stable for the human body and have no side effects, and conventional obesity inhibitors or metabolic diseases By confirming that it has an efficacy equal to or greater than that of a therapeutic substance, the present invention has been completed.

Republic of Korea Patent Publication No. 10-2016-0059152

It is an object of the present invention to provide a food composition for improving or preventing obesity or metabolic diseases, using red pepper leaf extract as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating obesity or metabolic diseases, comprising red pepper leaf extract as an active ingredient.

In order to achieve the above object, the present invention provides a food composition for improving or preventing obesity or metabolic disease, using red pepper leaf extract as an active ingredient.

The red pepper leaf extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The composition may be one that inhibits proliferative cell differentiation and fat accumulation, reduces an increase in body weight and body fat, lowers the concentration of endotoxin, triglyceride and total cholesterol in serum, and inhibits the expression of hormones related to fat differentiation in the serum. .

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating obesity or metabolic diseases, using red pepper leaf extract as an active ingredient.

The red pepper leaf extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The composition may be one that inhibits proliferative cell differentiation and fat accumulation, reduces an increase in body weight and body fat, lowers the concentration of endotoxin, triglyceride and total cholesterol in serum, and inhibits the expression of hormones related to fat differentiation in the serum. .

The composition of the present invention comprises a red pepper leaf extract, wherein the composition inhibits proliferative cell differentiation and fat accumulation, reduces an increase in body weight and body fat, lowers endotoxin, triglyceride and total cholesterol concentration in serum, and It has the effect of inhibiting the expression of hormones related to internal fat differentiation. In addition, the composition according to the present invention can be usefully used in functional foods and pharmaceuticals for improving, preventing and treating obesity or metabolic diseases, as well as the treatment of obesity by enhancing immune activity as well as obesity. have.

1 is an Oil red O staining result for confirming the change in lipid content in 3T3-L1 cells by treatment with hot water extract of red pepper leaf prepared from Example 1 and polymer fraction of red pepper leaf prepared from Example 2. FIG.
Figure 2 is a result of testing the effect of inhibiting adipocyte differentiation of the hot water extract of red pepper leaf prepared in Example 1 and the polymer fraction of red pepper leaf prepared in Example 2. FIG.
3 is a normal group (NC), a high-fat diet control (HF), a positive control (GC) in which 50 mg/kg of the garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And a graph showing the change in body weight over time in the 100 mg/kg administration group (WEPL100) of the red pepper leaf extract of Example 1.
4 is a normal group (NC), a high-fat diet control group (HF), a positive control group (GC) in which 50 mg/kg of garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And after extracting the white epididymis adipose tissue from the 100 mg/kg administration group (WEPL100) of red pepper leaf extract of Example 1, it is a graph showing the weight measured.
5 is a normal group (NC), a high-fat diet control group (HF), a positive control group (GC) in which 50 mg/kg of garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing by measuring the concentration of endotoxin in the serum extracted from the 100 mg / kg administration group (WEPL100) of red pepper leaf extract of Example 1.
6 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered with 50 mg/kg of garcinia cambogia extract of Comparative Example 1 (GC), and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And a graph showing the concentration of triglycerides in the serum extracted from the 100 mg/kg administration group (WEPL100) of the red pepper leaf extract of Example 1.
7 is a normal group (NC), a high-fat diet control group (HF), a positive control group (GC) in which 50 mg/kg of garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing by measuring the total cholesterol concentration in the serum extracted from the 100 mg / kg administration group (WEPL100) of red pepper leaf extract of Example 1.
Figure 8 is a normal group (NC), a high-fat diet control (HF), a positive control (GC) administered with 50 mg/kg of the garcinia cambogia extract of Comparative Example 1, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing by measuring the concentration of leptin in serum extracted from the 100 mg/kg administration group (WEPL100) of the red pepper leaf extract of Example 1.
9 is a normal group (NC), a high-fat diet control group (HF), a positive control group administered with 50 mg/kg of garcinia cambogia extract of Comparative Example 1 (GC), and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing by measuring the insulin concentration in the serum extracted from the 100 mg / kg administration group (WEPL100) of red pepper leaf extract of Example 1.

The present invention relates to a composition for improving, preventing or treating obesity or metabolic diseases, using red pepper leaf extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for improving, preventing or treating obesity or metabolic diseases, using red pepper leaf extract as an active ingredient.

Pepper ( Capsicum annuum ) is a tropical plant, classified as an annual plant belonging to the Solanaceae family , and is one of the most consumed vegetables worldwide. In Korea, it is cultivated mainly from late spring to summer, and has been used as a food ingredient for a long time as the main ingredient of kimchi. Currently, there are about 20 to 30 varieties of pepper known worldwide, of which Capsicum annuum, Capsicum frutescens, Capsicum chinense, Capsicum baccatum, and Capsicum pubescens are the most cultivated. Pepper is not only rich in vitamins and minerals, but it has been reported that it contains a large amount of phenolic compounds such as carotenoid, quercetin, luteolin and capsaicinoids, but studies on pepper leaves are insufficient.

In the present invention, the term'obesity' refers to a state in which body fat is accumulated excessively, rather than simply gaining a lot of weight. It means that even if it looks normal on the outside, if the percentage of body fat is high, it can be called obesity. Normally, the method of determining obesity is to use the body mass index (BMI). In the case of 23 to 24.9, overweight, 25 to 29.9 is determined only with mild obesity, 30 to 34.9 is determined as moderate obesity, and in the case of 35 or more, it is determined as high obesity. Are doing. Obesity is caused by a combination of multiple causes rather than a single cause.Including westernized eating habits, incorrect eating habits, decreased activity levels, emotional factors, and genetic factors can be cited.The resulting obesity eventually leads to chronic hyperlipidemia, diabetes, and high blood pressure. It increases the risk of developing the disease.

In the present invention, the term'metabolic disease' means a condition or disease that is closely related to or is caused by obesity, and is specifically selected from the group consisting of fatty liver, type 2 diabetes, hyperlipidemia, cardiovascular disease, and arteriosclerosis. There can be more than one.

In the present invention, fatty liver refers to a condition or disease in which fat is accumulated in an excessive amount in liver cells due to a disorder in the fat metabolism of the liver.

In the present invention, hyperlipidemia refers to a condition or disease in which the concentration of fat components, especially cholesterol and triglyceride in the blood is higher than the normal level, and includes all conditions requiring lowering the lipid concentration in the blood. Is used in a broad sense.

In the present specification, arteriosclerosis refers to a condition or disease in which blood circulation to organs and tissues in the body decreases due to thickening of the arterial wall and decreasing elasticity, and other substances such as fat and cholesterol are deposited on the inner wall of the artery and thus plaque ( Plaque) is formed to narrow the lumen, thereby reducing blood circulation, which means a condition or disease in which "atherosclerosis" is included. Arteriosclerosis can occur anywhere in the body, and if it occurs in the blood vessels in the heart, coronary artery disease such as angina pectoris and myocardial infarction can be caused, and cerebral infarction that occurs in the brain can be caused. Can be triggered.

Specifically, the composition for improving, preventing or treating obesity or metabolic disease may be referred to as a composition for improving, preventing, alleviating, suppressing, and treating weight or body fat increase or metabolic disease caused by the various causes. More preferably, it has the effect of improving, preventing, and treating obesity or metabolic diseases caused by a high fat diet.

According to the experimental results of the present invention, it can be seen that weight and body fat increased when a high fat diet was administered for a long time. It induces the differentiation of adipocytes and fat accumulation over time, increases the content of body weight and body fat, and increases the expression of hormones related to fat differentiation as well as endotoxin, triglyceride, and total cholesterol concentrations in the serum. Induce disease.

At this time, when the red pepper leaf extract is administered together, it suppresses the differentiation of fat cells and the accumulation of fat, reduces the increase in body weight and body fat, lowers the concentration of endotoxin, triglyceride and total cholesterol in the serum, and increases the expression of hormones related to fat differentiation in the serum. It can be confirmed that it suppresses. Therefore, it can be seen that the composition comprising the red pepper leaf extract according to the present invention as an active ingredient has an effect of improving, preventing or treating obesity or metabolic diseases.

In the present invention, the'extract' includes not only the crude extract obtained by treating the extraction raw material with the extraction solvent, but also the processed product of the crude extract. For example, the red pepper leaf extract may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the'extract' in the present invention also includes a fraction obtained by further fractionating the crude extract. That is, the red pepper leaf extract includes not only those obtained using an extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a polymer fraction obtained by passing through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. It can be purified through a method.

The polymer fraction may be a fraction obtained by separating only a polymer material having a molecular weight of 10 kDa or more after obtaining a red pepper leaf extract or a red pepper leaf extract using an extraction solvent.

In addition, in the present invention, the'pepper leaf extract' is an extract of the red pepper leaf using water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and the extraction method does not need to be particularly limited. Preferably, the red pepper leaf extract may be a hot water extract of red pepper leaf or an ethanol solution of red pepper leaf, and may be, for example, a red pepper leaf alcohol extract. Most preferably, it may be a hot water extract of red pepper leaves.

In addition, the red pepper leaf extract may be processed by the red pepper leaf extract, and then repeatedly extracted one or more times with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The red pepper leaves may be cut into a certain size, dried using a shade or a dryer, and then crushed or used as it is. The drying period is not particularly limited as long as the moisture content in the red pepper leaves is less than 20%.

Compared to other parts of red pepper, red pepper leaves are used to inhibit proliferative cell differentiation and fat accumulation, reduce weight and body fat gain, and reduce serum endotoxin, triglyceride, and total cholesterol concentrations when a high fat diet is administered for a long time. Since it is 1.5 to 2.5 times better in lowering and inhibiting the expression of hormones related to fat differentiation in the serum, red pepper leaf extract is remarkably excellent in preventing, improving or treating obesity or metabolic diseases compared to other parts of pepper.

In the present invention, "containing as an active ingredient" or "including as an active ingredient" means including an amount sufficient to improve, prevent or treat obesity or metabolic diseases of the red pepper leaf extract of the present invention. Since the red pepper leaf extract is a natural product and does not have side effects on the human body even if it is administered in an excessive amount, the upper and lower limits of the quantity of the red pepper leaf extract included in the composition of the present invention can be selected and carried out by a person skilled in the art within an appropriate range.

The composition of the present invention may be variously used as a food composition for improving and preventing obesity or metabolic diseases using the red pepper leaf extract of the present invention as an active ingredient. Formulated in the same manner as the pharmaceutical composition described below containing the red pepper leaf extract of the present invention as an active ingredient, it can be used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gum, ice cream, vitamin complexes, health supplements. Etc.

The food composition of the present invention may include not only red pepper leaf extract as an active ingredient, but also ingredients that are commonly added during food production, and include, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made of drinks and beverages, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts, in addition to the red pepper leaf extract of the present invention, may be further included.

The present invention provides a health functional food comprising a food composition for improving, suppressing or preventing obesity or metabolic disease, comprising the red pepper leaf extract as an active ingredient. Health functional foods are foods prepared by adding red pepper leaf extract to food materials such as beverages, teas, spices, chewing gums, confectionery, or encapsulating, powdering, or suspending them. However, unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be consumed on a daily basis. The amount of red pepper leaf extract added in such health functional foods cannot be uniformly regulated depending on the type of health functional food to be targeted, but can be added within the range that does not damage the original taste of the food. To 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of health functional foods in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In another example, as described above, it may be used as a pharmaceutical composition for preventing or treating obesity or metabolic diseases using red pepper leaf extract as an active ingredient. In addition to the red pepper leaf extract, which is an active ingredient, it can be prepared using a pharmaceutically suitable and physiologically acceptable auxiliary agent, and the auxiliary agent includes excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, lubricants or flavoring agents. Etc. can be used.

For administration, the pharmaceutical composition may include one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients, and may be preferably formulated into a pharmaceutical composition.

The formulation form of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops, or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders are, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, lacquercanth or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

As acceptable pharmaceutical carriers for compositions formulated as liquid solutions, sterilization and biocompatible, saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to prepare injectable formulations such as aqueous solutions, suspensions, emulsions, etc., pills, capsules, granules, or tablets.

Furthermore, it can be preferably formulated according to each disease or ingredient using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, and the like, preferably oral administration.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, mode of administration, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the patient, and is usually As such, a skilled physician can easily determine and prescribe an effective dosage for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g/kg, more preferably 0.1 g/kg to 10 g/kg.

The pharmaceutical composition of the present invention may be prepared in a unit dosage form by formulation using a pharmaceutically acceptable carrier and/or excipient, or may be prepared by placing it in a multi-dose container. At this time, the formulation may be in the form of a solution, suspension, or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or a stabilizer.

In addition, the present invention provides a method for improving, preventing or treating obesity or metabolic diseases, comprising administering an effective amount of red pepper leaf extract to a mammal.

The term "mammal" as used herein refers to a mammal that is the subject of treatment, observation or experimentation, and preferably refers to a human.

The term "effective amount" as used herein refers to an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, which is considered by a researcher, veterinarian, doctor or other clinician, which Contains an amount that induces relief of the. The effective amount and the number of administrations for the active ingredient of the present invention may vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by a person skilled in the art, and the weight, body fat, triglyceride and cholesterol in the serum or the degree of obesity or metabolic disease, the content of active ingredients and other ingredients contained in the composition, the type of formulation, And the patient's age, weight, general health status, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and drugs used simultaneously. In the prevention, treatment or improvement method of the present invention, in the case of an adult, when the red pepper leaf extract is administered once to several times a day, it is preferable to administer it in a dose of 0.001 g/kg to 10 g/kg, and more preferably It may be administered in a dose of 0.1 g / kg to 10 g / kg.

In the treatment method of the present invention, the composition comprising the red pepper leaf extract as an active ingredient is in a conventional manner through oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. It can be administered, and can be preferably administered orally or intraperitoneally.

Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention, but the following examples are only illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It is natural that such modifications and modifications fall within the appended claims.

Example 1. Hot water extract of red pepper leaves

Dried domestic red pepper leaves produced by Yecheon Nonghyup in Gyeongbuk were purchased and used. Red pepper leaves were dried in a drying oven at 50° C. for 24 hours and then pulverized to a size of 1.0 mm or less with a grinder to prepare red pepper leaf powder.

Distilled water equivalent to 20 times the volume was added to 5 g of red pepper leaf powder, followed by reflux extraction at 80° C. for 3 hours to obtain a hot water extract. After filtering the hot water extract, it was concentrated under reduced pressure using a rotary vacuum evaporator, and lyophilized for 72 hours. The hot water extract of pepper leaves obtained by the above method was described as WEPL (Water-soluble extract from pepper leaves).

Example 2. Red pepper leaf polymer fraction

Dried domestic red pepper leaves produced by Yecheon Nonghyup in Gyeongbuk were purchased and used. Red pepper leaves were dried in a drying oven at 50° C. for 24 hours and then pulverized to a size of 1.0 mm or less with a grinder to prepare red pepper leaf powder.

Distilled water corresponding to 20 times the volume was added to 100 g of red pepper leaf powder, followed by reflux extraction at 80° C. for 3 hours to obtain a hot water extract. After filtering the hot water extract, it is concentrated under reduced pressure to be 1/10 of the extract using a rotary vacuum evaporator, and 80% alcohol corresponding to 4 times the volume (v/v) of the hot water extract concentrated under reduced pressure Was added to the hot water extract and precipitated for 24 hours. This was centrifuged (6000 rpm, 635 × g, 30 minutes) to obtain a precipitate, and a small amount of distilled water was added thereto to redissolve the precipitate and a dialysis membrane (MW cut off 12,000, Sigma-Aldrich Co., St. Louis, MO, USA) was performed for 2 to 3 days to remove low molecular weight substances, and freeze-dried for 72 hours to obtain a polymer fraction of red pepper leaves. The red pepper leaf polymer fraction obtained by the above method was named HFPL (High-molecular fraction from pepper leaves).

Comparative Example 1. Garcinia cambogia extract

Garcinia cambogia extract was purchased and used from Unicon Natural Products PVT, India. At this time, the garcinia cambogia extract was used that contains 600 mg/g or more of HCA.

<Test Example>

Test Example 1. Analysis of yield, sugar and protein content of hot water extract of red pepper leaf of Example 1 and polymer fraction of red pepper leaf of Example 2

For the analysis of neutral sugars, the method of Albersheim et al. was partially modified to derivatize each constituent sugar with alditol acetate and aldonolactone after hydrolysis, and GC (Gas Chromatography ACME-6100, Young-Lin Co. Ltd. Anyang, Korea) ) Was used. The sample of Example 1 or 2 was hydrolyzed by reacting for 1.5 hr at 121° C. in 2 M TFA (trifluroacetic acid), and then dissolved in 1 ml of 1 M NH ₄ OH (ammonia solution) and 10 mg of NaBH ₄ It was reduced by 4 hr. After adding an appropriate amount of acetic acid to remove the remaining NaBH ₄ , methanol was added thereto and repeated drying to remove excess acetic acid and converted to alditol. 1 ml of acetic anhydride was added to the alditol and reacted at 121° C. for 30 min to convert to alditol acetate, which was separated and extracted with a chloroform/H ₂ O two-phase solvent system. And, the extract was dried and dissolved in a small amount of acetone to be used as a sample for GC analysis.

Protein quantification was measured according to the Bradford method, and BSA (Bovine Serum Albumin, sigma aldrich) was used as a standard and diluted at a constant multiple with 1 mg/ml as the highest concentration. After adding 20 µl of each standard and sample to 980 µl of Bradford reagent, the absorbance was measured at 595 nm using a microplate reader. Protein quantification was converted to the amount for BSA.

Total sugar was measured according to the phenol-sulfuric acid method, and after stirring the same amount of 5% phenol solution to 0.5 mL of the sample solution, 2.5 mL of concentrated sulfuric acid (98%, v/v) was added and reacted at room temperature for 20 minutes to make a microplate. Absorbance was measured at 470 nm using a reader. For the determination of total sugar, a calibration curve was prepared using a glucose standard (sigma aldrich) and converted into the amount.

Total polyphenol content was analyzed according to the Folin Denis method using gallic acid (sigma aldrich).

In the case of constituent sugar, the oligosaccharides of the sample were hydrolyzed to monosaccharides, and then HPAEC-PAD (high-performance anion-exchange chromatography-pulsed amperometric detector) was used, and the data were analyzed using Chromelon 6.80 software (Dionex). Monosaccharides (fucose, rhamnose, arabinose, galactose, glucose, xylose) were used as standard solutions.

As a result, as a result of analyzing the physicochemical properties such as the content of main components of the hot water extract and the polymer fraction from red pepper leaves, it is shown in Table 1 below.

division Example 1 (WEPL) Example 2 (HFPL) Extraction yield(%) 12.66 2.68 Chemical composition(%) Protein 4.5 ± 0.2 4.3 ± 0.4 Neutral sugar 70.7 ± 2.2 52.4 ± 0.5 Uronic acid 16.9 ± 0.1 36.7 ± 1.1 KDO-liked material 0.1 ± 0.1 2.5 ± 0.5 polyphenol 7.8 ± 0.4 4.0 ± 0.7 Component sugar (PMP) (Mole %) Rhamnose (Rhamnose) 9.3 ± 0.1 3.6 ± 0.1 Fucose (fucose) - 0.2 ± 0.0 Arabinose (Arabinose) 2.5 ± 0.2 4.9 ± 0.2 Xylose 0.6 ± 0.1 0.6 ± 0.1 Mannose 0.8 ± 0.0 0.8 ± 0.0 Galactose 8.5 ± 0.1 7.1 ± 0.1 Glucose 54.8 ± 0.5 34.6 ± 0.6 Glucuronic acid - 1.5 ± 0.0 Galacturonic acid 11.1 ± 0.8 36.0 ± 1.1

* The content ratio (%) of the chemical component represents the ratio in the dry sample.

As shown in Table 1, the hot-water extract of red pepper leaves prepared from Example 1 was found to have a yield of 12.66%, a protein content of 4.5% by weight, and a neutral sugar content of 70.7% by weight, indicating a high sugar content. In addition, as a result of analyzing the constituent sugars of the hot water extract of red pepper leaves prepared in Example 1, the main constituent sugar was glucose (54.8 Mole %), and galacturonic acid, rhamnose, and galactose were contained in 11.1, 9.3, and 8.5 Mole%, respectively. , arabionose, mannose, and xylose were found to be present in trace amounts of 2.5, 0.8 and 0.6 Mole%, respectively.

On the other hand, the yield of the red pepper leaf polymer fraction prepared in Example 2 was found to be as low as 2.68%, but it was confirmed that the protein content was 4.3% by weight, the neutral sugar content was 52.4% by weight, and the uronic acid was 36.7% by weight. In addition, in the red pepper leaf polymer fraction prepared from Example 2, the constituent sugar content of galacturonic acid and glucose was detected as 36.0 and 34.6 Mole%, respectively, and galactose, arabinose, rhamnose, glucuronic acid, mannose, xylose, and fucose were 7.1, 4.9, respectively. It was confirmed that there were traces of 3.6, 1.5, 0.8, 0.6, and 0.2 Mole %. It was confirmed that the sugar content of the red pepper leaf polymer fraction (HFPL) of Example 2 was reduced overall compared to the hot water extract of red pepper leaf of Example 1, but the contents of Arabinose, Fucose, and Glucuronic acid slightly increased. That is, it can be seen that there is a clear difference between the specific components present in the two extracts.

Test Example 2. Analyzing the efficacy of inhibiting differentiation of all cells and inhibiting fat accumulation

It was attempted to confirm the obesity inhibitory effect from the hot water extract of red pepper leaf of Example 1 and the polymer fraction of red pepper leaf of Example 2.

1) cell culture

For the experiment, 3T3-L1 whole fat cells were distributed from the American Type Culture Collection (ATCC), and 10% fetal bovine serum (FBS), 1% penicillin and strptomycin (Gibco BRL, Grand Island, NY, USA) containing DMEM (Dulbecco's Modified EaDCRT Media (Gibco BRL)) was cultured under conditions of 37°C and 5% CO ₂ . In order to solve the over-density phenomenon caused by the proliferation of the number of cells, the growth medium was exchanged every 48 hours to maintain an appropriate number of cells.

(2) eruption induction

3T3-L1 cells were dispensed into a 6-well plate at a density of 0.8×10 ⁵ cells/well, and subcultured using 0.05% Trypsin-EDTA every 48 hours. To induce differentiation into adipocytes, MDI (0.5 mM 3-isobutyl-1-methylxanthin, IBMX), 1 μM dexamethasone, 10 μg/mL insulin ) And 10 vol% fetal bovine serum (FBS) were incubated for 48 hours in DMEM differentiation medium. Thereafter, the culture was continued until the differentiation was completed while solidifying every 2 days with a medium (DMEM) containing only 10 μg/mL insulin. In order to confirm the effect of the hot water extract of red pepper leaf and the polymer fraction of red pepper leaf in the process of adipogenesis, the hot water extract of red pepper leaf of Example 1 50 μg/ml (WEPL-50) and 100 μg/ml (WEPL-100) of Example 1 whenever the medium was changed. , Red pepper leaf polymer fraction of Example 2 50 μg/ml (HFPL-50) and 100 μg/ml (HFPL-100) and Garnisia cambogia extract of Comparative Example 1 50 μg/ml (GC-50) and 100 μg/ml (GC-100) was treated together.

When the medium was exchanged, the control (C) treated with MDI (0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 1 μM dektamethasone, 10 μg/mL insulin), which is not an extract, but a lipid accumulation inducing factor. ), and treated with only a medium (DMEM) containing only 10 μg/mL insulin without any treatment, was used as a normal group (NC).

(3) Oil Red O staining and quantification

On the 8th day when adipocyte differentiation was completed, each cell was recovered, and the adipogenesis inhibitory activity was evaluated therefrom as follows. To quantitatively analyze the fat accumulation amount of 3T3-L1 cells differentiated into each adipocyte by Oil Red O staining, wash with PBS, fix with 3.7% formalin for 10 minutes, and wash with 60% isopropanol. Thereafter, each well was completely dried. Oil red O dye was treated at room temperature for 20 minutes, washed 4 times with distilled water, and then the number and size of lipid droplets of 3T3-L1 adipocytes differentiated from each group were measured under a microscope (Eclipse ti, Nikon). Observed through. For quantitative analysis, fat was extracted using 100% isopropanol, transferred to a 96-well plate 200 μl each, and the absorbance was measured at 500 nm with an ELISA reader, and expressed as a percentage of the absorbance value of the control group.

(4) Effect of red pepper leaf extract on the formation of adipocytes in 3T3-L1 adipocytes

It was investigated whether the formation of lipid droplets appearing in the process of differentiation of 3T3-L1 adipocytes into adipocytes was inhibited by treatment with hot water extract of red pepper leaf or polymer fraction of red pepper leaf.

1 is an Oil red O staining result for confirming the change in lipid content in 3T3-L1 cells by treatment with hot water extract of red pepper leaf prepared from Example 1 and polymer fraction of red pepper leaf prepared from Example 2. FIG. Figure 2 is a result of testing the effect of inhibiting adipocyte differentiation of the hot water extract of red pepper leaf prepared in Example 1 and the polymer fraction of red pepper leaf prepared in Example 2. FIG.

As shown in Figs. 1 and 2, it can be seen that fat synthesis was suppressed in both the WEPL group or the HFPL group than the control group (C) in which lipid accumulation was induced. In particular, in the WEPL-100 group, the effect of inhibiting adipocyte differentiation and adipogenesis was the most excellent.

According to FIG. 2, the GC-50 group treated with the garcinia cambogia extract (Comparative Example 1) compared to the control group (C) inducing lipid accumulation showed about 10.3% fat accumulation inhibitory ability, and the WEPL-50 group and WEPL- The 100 groups showed 1.9% and 7.6% of fat accumulation inhibition, respectively. In addition, the HFPL-50 group and the HFPL-100 group showed the ability to inhibit fat accumulation of 3.0% and 13.7%, respectively. Taken together, the red pepper leaf extract according to the present invention, as well as the red pepper leaf polymer fraction, exhibited the effect of inhibiting differentiation and adipogenesis from 3T3-L1 adipocytes to adipocytes, which was confirmed to be at the same level as the conventional garcinia cambogia extract. In the case of garcinia cambogia extract, it is known to cause side effects in the human body, so there is a limit to its performance, but the red pepper leaf extract or the red pepper leaf polymer fraction of the present invention has the advantage that there is no limitation in utilization because the red pepper leaf extract or the red pepper leaf polymer fraction of the present invention hardly appears in vivo.

Test Example 3. Analysis of factors related to obesity and metabolic diseases of hot water extract of red pepper leaves of Example 1

(1) Experimental animals and diet

45 6-week-old male C57BL/6J-based mice bred in a sterile environment were purchased from Central Experimental Animal Co., Ltd., and the mouse breeding conditions were kept at a temperature of 22 ± 2 ℃ and a humidity of 40-60%. Light and dark cycle) was adjusted at 12 hour intervals.

The mice were divided into 5 groups of 9 mice each, and an experimental diet was supplied and reared for 8 weeks. The experimental diet was a high fat diet, and a modified AIN 76A purified rodent diet (60 cal% fat), which increased calories by 60 cal% by adding Lard, was fed freely, and the normal group (NC), the high fat diet control group (HF), a positive control group (GC) in which 50 mg/kg of garcinia cambogia extract of Comparative Example 1 was administered, a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50), and a 100 mg/kg administration group of red pepper leaf extract of Example 1 The experimental group was divided into (WEPL100) and the experiment was conducted. Water and the experimental diet were freely ingested, and the weight gain during the experiment was measured at 10 am at 1 week intervals for 8 weeks from the start of the experimental diet. The garcinia cambogia extract of Comparative Example 1 and the red pepper leaf extract of Example 1 were each dissolved in sterile saline and administered orally.

(2) Collection of blood and organs

After the experiment was completed, the experimental animals were fasted for 12 hours, anesthetized with iFran solution, the abdomen and chest cavity were incised to coagulate the blood collected from the portal vein, and then the serum was separated and the concentration of serum lipids and obesity-related hormones Was used to analyze. White epididymal adipose tissue and other organs were removed from each experimental animal, blood and foreign substances were removed using physiological saline, weighed, and stored at -80°C until analysis.

(3) weight change and body fat mass analysis

3 is a normal group (NC), a high-fat diet control (HF), a positive control (GC) in which 50 mg/kg of the garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing the weight change over time in the 100 mg / kg administration group (WEPL100) of red pepper leaf extract of Example 1, Figure 4 is a normal group (NC), high fat diet control (HF), the garcinia cambogia extract of Comparative Example 1 50 After extracting white epididymal adipose tissue from the positive control group administered with mg/kg (GC), the red pepper leaf extract 50 mg/kg administration group (WEPL50) of Example 1, and the red pepper leaf extract 100 mg/kg administration group (WEPL100) of Example 1, It is a graph showing the weight measured.

As shown in FIG. 3, compared to the normal group (NC), the high fat diet control group (HF) showed a significantly higher increase in body weight (11.9 g increase). On the other hand, the group to which the garcinia cambogia extract of Comparative Example 1 and the red pepper leaf extract of Example 1 were administered orally showed a tendency to significantly decrease body weight compared to the high-fat diet control group (HF). Specifically, the group (GC) to which 50 mg/kg of Garnisia cambogia extract (Comparative Example 1) was orally administered while on a high fat diet (GC) lost 26.9% body weight compared to the high fat diet control group (HF), and red pepper leaf extract 50 while on a high fat diet The mg/kg administration group (WEPL50) and the 100 mg/kg administration group (WEPL100) lost 25.1% and 28.5% body weight, respectively, compared to the high fat diet control group (HF). In particular, the WEPL-100 group to which 100 mg/kg of the red pepper leaf extract of Example 1 was orally administered had a significant decrease in body weight compared to the positive control group (GC group) (p<0.05).

As shown in FIG. 4, the amount of white epididymal fat extracted from each experimental group was significantly increased in the high-fat diet control group (HF) compared to the normal group (NV). It was confirmed that the weight of the white epididymal adipose tissue was reduced by about 2.7% and 16.1% in the WEPL-50 group and the WEPL-100 group to which the pepper leaf extract of Example 1 was administered compared to the high fat diet control group (HF). Therefore, it can be seen that the red pepper leaf extract of the present invention has an effect of reducing the weight of adipose tissue, and suppresses an increase in body fat as well as weight loss.

(4) Plasma lipid content analysis

The concentrations of endotoxin, triglyceride, and total cholesterol in serum were analyzed by enzyme method using a blood analysis kit of Shinyang Chemical Co., Ltd. Triglycerides were analyzed by Tiglyzyme-V and total cholesterol by Cholestezyme-V. Pierce™ LAL Chromogenic Endotoxin Quantitation Kit (Thermo scientific) was used to determine the amount of Endotoxin in mouse serum.

5 is a normal group (NC), a high-fat diet control group (HF), a positive control group (GC) in which 50 mg/kg of garcinia cambogia extract of Comparative Example 1 was administered, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And it is a graph showing by measuring the concentration of endotoxin in the serum extracted from the 100 mg / kg administration group (WEPL100) of red pepper leaf extract of Example 1, Figure 6 is a normal group (NC), a high-fat diet control group (HF), Garcinia of Comparative Example 1 Neutrality in serum extracted from the positive control group (GC) administered with 50 mg/kg of Cambogia extract, 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) and 100 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL100) It is a graph showing the measurement of fat concentration, and FIG. 7 is a normal group (NC), a high-fat diet control (HF), a positive control (GC) administered with 50 mg/kg of garcinia cambogia extract of Comparative Example 1, and red pepper leaves of Example 1 It is a graph showing the total cholesterol concentration in the serum extracted from the extract 50 mg/kg administration group (WEPL50) and the pepper leaf extract 100 mg/kg administration group (WEPL100) of Example 1.

As shown in Figure 5, endotoxin increased 106.3% in the high fat diet control group (HF) compared to the normal group (NC), but the WEPL-50 group and the WEPL-100 group were 14.6% and respectively compared to the high fat diet control group (HF). Since the decrease was 32.9%, it was confirmed that the red pepper leaf extract of Example 1 reduced the concentration of endotoxin in the serum to suppress obesity and at the same time prevent inflammation induced by obesity.

6 and 7, triglyceride and total cholesterol were significantly increased in the high fat diet control group (HF) compared to the normal group (NC), but the WEPL-50 group and the WEPL-100 group were compared to the high fat diet control group (HF). Significantly decreased. The red pepper leaf extract of Example 1 according to the present invention was only co-administered with a high fat diet, and showed triglyceride and total cholesterol similar to those of the normal group (NC), and it can be seen that the obesity inhibitory effect is remarkably excellent.

(5) Plasma fat differentiation related hormones (leptin, insulin)

The concentrations of leptin (mouse leptin ELISA kit, R&D systems, Minneapolis, USA) and insulin (mouse insulin ELISA kit, ALPCO, New Hampshire, USA) were analyzed using ELISA assay.

Figure 8 is a normal group (NC), a high-fat diet control (HF), a positive control (GC) administered with 50 mg/kg of the garcinia cambogia extract of Comparative Example 1, and a 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) And a graph showing the concentration of leptin in serum extracted from the 100 mg/kg administration group (WEPL100) of the red pepper leaf extract of Example 1, and FIG. 9 is a normal group (NC), a high-fat diet control group (HF), and a garcinia of Comparative Example 1. Insulin in serum extracted from positive control (GC) administered with 50 mg/kg of Cambogia extract, 50 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL50) and 100 mg/kg administration group of red pepper leaf extract of Example 1 (WEPL100) This is a graph showing the concentration measured.

As shown in FIGS. 8 and 9, leptin is a hormone produced and secreted by adipose tissue, and it is known to be involved in energy metabolism and weight control by suppressing appetite and increasing energy consumption in proportion to the amount of body fat in blood. The leptin concentration decreased by 31.8%, 18.2%, and 39.1% in the GC group, WEPL-50 group, and WEPL-100 group, respectively, compared to the high fat diet control group (HF). In addition, as a result of measuring the serum insulin concentration, the GC group, WEPL-50 group, and WEPL-100 group decreased by 32.3%, 18.1% and 38.9%, respectively, compared to the high fat diet control group (HF). Therefore, the red pepper leaf extract of Example 1 showed a result of significantly reducing the concentration of leptin and insulin in the serum, which is associated with a decrease in body fat mass.

When the above results are summarized, the red pepper leaf extract of Example 1 is confirmed to exhibit an improvement, prevention or treatment effect on obesity when 50 mg/kg or more is used, but it is more preferable because it shows the best effect when it is 100 mg/kg or more. , Since there is no increase in the effect when used in a concentration of 10 g/kg or more, it is most preferably used in a concentration of 100 to 10,000 mg/kg.

Hereinafter, examples of the formulation of the composition containing the powder of the present invention are described, but the present invention is not intended to limit it, but is intended to be described in detail.

Formulation Example 1. Preparation of powder

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 500 mg

100 mg lactose

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2. Preparation of tablet

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 300 mg

Corn starch 100 mg

Lactose 100 mg

2 mg of magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet preparation method.

Formulation Example 3. Preparation of Capsule

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 200 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare a capsule.

Formulation Example 4. Preparation of injection

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 600 mg

Mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

It is prepared with the above ingredients per ampoule according to a conventional injection preparation method.

Formulation Example 5. Preparation of liquid formulation

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 4 g

10 g of isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to the usual preparation method of liquid preparation, add each ingredient to purified water to dissolve it, add lemon zest, mix the above ingredients, add purified water and add purified water to adjust the total to 100g, then fill in a brown bottle for sterilization. To prepare a solution.

Formulation Example 6. Preparation of granules

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 1,000 mg

Vitamin mixture right amount

Vitamin A acetate 70 ㎍

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 ㎍

Vitamin C 10 mg

Biotin 10 ㎍

Nicotinic acid amide 1.7 mg

Folic acid 50 ㎍

0.5 mg of calcium pantothenate

Suitable amount of inorganic mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dicalcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules, but it is possible to arbitrarily modify the mixing ratio. After mixing the above ingredients according to a conventional granule preparation method, granules And can be used to prepare a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

Hot water extract of red pepper leaves obtained in Example 1 or polymer fraction of red pepper leaves obtained in Example 2 1,000 mg

Citric acid 1,000 mg

100 g oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 ml by adding purified water

After mixing the above ingredients according to a normal health drink manufacturing method, stirring and heating the resulting solution at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and stored in a refrigerator. It is used to prepare the functional beverage composition of the invention.

The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences, such as the demand class, the country of demand, and the purpose of use.

Claims (6)

A food composition for improving or preventing obesity or metabolic diseases using red pepper leaf extract as an active ingredient. The method of claim 1,
The red pepper leaf extract is a food composition for improving or preventing obesity or metabolic diseases, characterized in that the extract is extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The method of claim 1,
The composition is characterized in that it inhibits proliferative cell differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers the concentration of endotoxin, triglyceride and total cholesterol in the serum, and inhibits the expression of hormones related to fat differentiation in the serum. A food composition for improving or preventing obesity or metabolic diseases. A pharmaceutical composition for preventing or treating obesity or metabolic diseases using red pepper leaf extract as an active ingredient. The method of claim 4,
The red pepper leaf extract is a pharmaceutical composition for preventing or treating obesity or metabolic diseases, characterized in that the extract is extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The method of claim 4,
The composition is characterized in that it inhibits proliferative cell differentiation and fat accumulation, reduces the increase in body weight and body fat, lowers the concentration of endotoxin, triglyceride and total cholesterol in the serum, and inhibits the expression of hormones related to fat differentiation in the serum. A pharmaceutical composition for preventing or treating obesity or metabolic disease or metabolic disease.

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