KR20210086176A - Functional Cosmetic Composition Comprising Psidium Guajava Leaf Extract And Ipomoea Batats Extract And Method For Producing Same - Google Patents

Abstract

The present invention relates to a functional cosmetic composition comprising a Psidium guajava leaf extract and an Ipomoea batatas extract, and a method for preparing the same and, more particularly, to a functional cosmetic composition comprising a Psidium guajava leaf extract and an Ipomoea batatas extract, which have antioxidant activity, whitening, and cell protection effects, by mixing the Psidium guajava leaf extract and the Ipomoea batatas extract in a certain ratio, and a method for preparing the same.

Description

Functional Cosmetic Composition Comprising Psidium Guajava Leaf Extract And Ipomoea Batats Extract And Method For Producing Same

The present invention relates to a functional cosmetic composition comprising a guava leaf extract and a sweet potato extract, and a method for preparing the same, and more particularly, by mixing a guava leaf extract and a sweet potato extract in a certain ratio, antioxidant activity, whitening effect and cell protection It relates to a composition for a functional cosmetic comprising an effective guava leaf extract and a sweet potato extract, and a method for manufacturing the same.

Recently, in order to reduce skin irritation caused by various chemical substances, a lot of attention has been paid to functional cosmetics obtained from natural extracts, which have functions such as antioxidant, wrinkle relief, and whitening. Natural ingredients have few side effects on the skin, and as consumers' response to external skin preparations using natural ingredients increases, the value of development as a raw material for external preparations for skin is increasing.

Guava (Psidium cattleianum Sabine) belongs to the Myrtaceae family, which includes more than 133 genera and more than 3,800 plant species. Guava leaves have also been used in African folk medicine for the treatment of diabetes, diarrhea, cough, menstrual cramps and high blood pressure. In addition, it acts as a wound infection inhibitor and antiseptic, and has been used for the treatment of acne, dental caries, gum infections and sore throat.

Previous studies on the efficacy of these guava plants include antibacterial effect, anti-diarrheal effect, anti-diabetic effect, anti-acne effect, sedative, anti-cough effect, and narcotic activity.

According to many research results, it has been reported that guava leaves contain a large amount of triterpenoids, flavonoids, essentialoils and tannins. It has been reported to exhibit the effect, sperm protection effect, and the effect of preventing damage by chemicals.

On the other hand, sweet potato (Ipomoea batatas) is a dicotyledonous perennial plant of the callaceae family Convolvulaceae, also called Gamjeo, which is native to South America, but is currently cultivated by expanding its distribution to Asian regions such as Korea, China, and Indonesia. It is one of the most important food crops worldwide.

In the case of such sweet potatoes, the root of the sweet potato, that is, the tuber, mainly composed of starch, is used as a source of carbohydrates such as food. The tubers of the sweet potato are rich in various vitamins, minerals and extracts, etc. in addition to starch, and the tubers of the sweet potato are not only used for food, but also recently corrosive or snack or industrial starch, alcoholic beverages such as shochu or whiskey, alcohol, cosmetics or pharmaceuticals. is used as a raw material for

On the other hand, there have been attempts such as using each of the guava leaf extract and the sweet potato extract as cosmetic raw materials, but there has been no research on cosmetic raw materials including guava leaf extract and sweet potato extract as active ingredients at the same time.

Accordingly, it is an object of the present invention to solve the problems according to the prior art.

Specifically, it is an object of the present invention to provide a cosmetic composition having an antioxidant effect, a whitening effect and a cell protective effect using a guava leaf extract and a sweet potato extract, and a method for preparing the same.

According to an embodiment of the present invention for solving the above problems, the present invention is characterized in that it contains a mixture of guava leaf extract (Psidium guajava L.) and sweet potato extract (Ipomoea batatas) as an active ingredient.

More preferably, the guava leaf extract or the sweet potato extract is characterized in that the concentration of 0.01% (w / v) to 0.09% (w / v) with respect to the mixture.

More preferably, the guava leaf extract is at a concentration of 0.025% (w/v) with respect to the mixture, and the sweet potato extract is characterized in that the concentration is 0.075% (w/v) with respect to the mixture.

More preferably, the mixture is characterized in that it is in the form of any one selected from a lotion, a nourishing lotion, a nourishing cream, a massage cream, a pack, an emulsified foundation, and a nourishing essence.

More preferably, a freeze-dried product preparation step of pulverizing a freeze-dried product of guava leaves and a freeze-dried product of sweet potato, a mixed solution preparation step of dissolving the pulverized freeze-dried product in distilled water at a certain ratio to prepare a mixed solution, After the mixed solution preparation step , a centrifugation step of centrifugation and, after the centrifugation step, characterized in that it comprises a mixture extraction step of extracting the mixture by filtering the supernatant.

More preferably, the mixture produced in the mixture extraction step is characterized in that it contains a guava leaf extract and a sweet potato extract as active ingredients.

More preferably, the step of preparing the mixed solution is characterized in that the sum of the concentrations of the guava leaf extract and the sweet potato extract is 0.1% (w/v).

According to the means for solving the above-mentioned problems, the functional cosmetic composition comprising the guava extract and the sweet potato extract according to the present invention as an active ingredient and a method for producing the same, are non-toxic, have no side effects, and are composed of a natural extract harmless to the human body, thereby providing excellent antioxidant effect , whitening effect and cell protection effect, there is an advantage in that it is harmless to consumers and can provide a functional cosmetic with excellent efficacy.

1 is a graph showing the absorbance for confirming the antioxidant effect of a mixture comprising a guava leaf extract and a sweet potato extract according to the present invention.
2 is a graph showing the absorbance for confirming the antioxidant effect of the guava leaf extract according to the present invention.
3 is a graph showing the absorbance for confirming the antioxidant effect of the sweet potato extract according to the present invention.
4 is a graph showing the absorbance for confirming the whitening effect of a mixture comprising a guava leaf extract and a sweet potato extract according to the present invention.
5 is a graph showing the absorbance for confirming the cytoprotective effect of the guava leaf extract and the sweet potato extract according to the present invention.
6 is a flowchart schematically illustrating the steps of preparing a mixture comprising a guava leaf extract and a sweet potato extract as active ingredients according to the present invention.

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings.

However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only these embodiments allow the disclosure of the present invention to be complete, and are common in the art to which the present invention pertains. It is provided to fully inform those with knowledge of the scope of the invention, and the present invention is only defined by the scope of the claims.

In addition, the terminology used herein is for the purpose of describing the embodiments and is not intended to limit the present invention.

As used herein, the singular also includes the plural unless specifically stated otherwise in the phrase. As used herein, "comprises" and/or "comprising" does not exclude the presence or addition of elements other than those mentioned.

Unless otherwise defined, all terms (including technical and scientific terms) used herein may be used with the meaning commonly understood by those of ordinary skill in the art to which the present invention belongs.

The present invention relates to a cosmetic composition comprising a mixture of guava leaf extract (Psidium guajava L.) and sweet potato extract (Ipomoea batatas) as an active ingredient.

In order for the cosmetic composition of the present invention to exhibit antioxidant, whitening and cytoprotective effects, the guava leaf extract has a concentration of 0.01% (w/v) to 0.09% (w/v) with respect to the mixture, and the sweet potato extract is It is preferable to have a concentration of 0.01% (w/v) to 0.09% (w/v) with respect to the mixture.

Here, when the concentration of the guava leaf extract is less than 0.01% (w/v), the above-described antioxidant, whitening and cytoprotective effects are not effectively expressed, and the concentration of the guava leaf extract is 0.09% (w/v) If it exceeds, the manufacturing cost and time required for the mixture may be excessively required.

In addition, when the concentration of the sweet potato extract is less than 0.01% (w/v), the above-described antioxidant, whitening and cytoprotective effects are not effectively expressed, and the concentration of the sweet potato extract exceeds 0.09% (w/v). In this case, the manufacturing cost and time required for the mixture may be excessively required.

In more detail, the guava leaf extract has a concentration of 0.025% (w / v) with respect to the mixture, and the sweet potato extract has a concentration of 0.075% (w / v) with respect to the mixture of the guava leaf extract and the sweet potato extract. When the ratio is 3:1, the guava leaf extract has a concentration of 0.05% (w/v) with respect to the mixture, and the sweet potato extract has a concentration of 0.05% (w/v) with respect to the mixture It is most preferable when the ratio of the guava leaf extract and the sweet potato extract is 1:1.

More specifically, when the concentration of the guava leaf extract is 0.075% (w/v) and the concentration of the sweet potato extract is 0.025% (w/v), the antioxidant effect is more strongly expressed, and the When the concentration is 0.05% (w/v) and the concentration of the sweet potato extract is 0.05% (w/v), the whitening effect is more strongly expressed. , based on the drawings (experimental data) and examples to be described later, will be described later in more detail.

In addition, the cosmetic composition of the present invention may be prepared in any one form selected from a lotion, a nourishing lotion, a nourishing cream, a massage cream, a pack, an emulsified foundation, and a nourishing essence, and may be prepared in any form.

In addition, the cosmetic composition of the present invention may use each or a mixture of guava leaf extract or sweet potato extract as an active ingredient, which can be easily determined by those skilled in the art.

[Manufacturing method]

The extract of the present invention is preferably prepared by the following preparation method, but is not limited thereto.

6 is a flowchart schematically illustrating the steps of preparing a mixture comprising a guava leaf extract and a sweet potato extract as active ingredients according to the present invention.

As shown in Figure 6, the extract according to the present invention can be prepared through the freeze-dried material preparation step (S100), the mixed solution preparation step (S200), the centrifugation step (S300) and the mixture extraction step (S400).

To explain each step, first, the freeze-dried material preparation step (S100) is a process of pulverizing the freeze-dried guava leaf and the freeze-dried sweet potato.

Here, the freeze-dried products of guava leaves and sweet potatoes can be used without limitation, such as cultivated or commercially available ones, and in the present invention, they can be appropriately minced and pulverized.

Next, the mixed solution preparation step (S200) is a process of preparing a mixed solution by dissolving the pulverized freeze-dried product in distilled water at a certain ratio.

More specifically, in the mixed solution preparation step (S200), the freeze-dried product of guava leaves and sweet potato is sufficiently dissolved in distilled water, and the freeze-dried product is dissolved so that the ratio of the freeze-dried product has a concentration of 0.1% (w/v) compared to distilled water.

That is, the mixed solution is sufficiently mixed so that when distilled water is 100, the guava leaf extract and the sweet potato extract have a ratio of 0.1 compared to the distilled water.

In addition, the ratio of the guava leaf extract and the sweet potato extract of the mixture is preferably an optimal concentration according to the result data of Examples according to each efficacy to be described later, and when out of the corresponding range, the optimal antioxidant effect and whitening effect according to the present invention may not be able to represent

Next, the centrifugation step (S300) is a step of centrifuging after the step of preparing the mixed solution (S200).

In addition, as the mixed solution extraction method, any extraction known in the art may be used, but extraction through centrifugation may be preferably used. More specifically, after centrifuging a mixed solution obtained by dissolving a freeze-dried product of guava leaves and sweet potatoes in distilled water with a centrifugal separator, the supernatant is filtered and finally a mixture containing guava leaf extract and sweet potato extract for a cosmetic composition as active ingredients can be manufactured.

That is, the mixture extraction step (S400) is a process of extracting the mixture by filtering the supernatant after the centrifugation step, and finally, a mixture for a cosmetic composition can be prepared.

In addition, in the present invention, the guava leaf extract and sweet potato extract can be extracted according to a conventional method known in the art for extracting extracts from natural products, that is, using a conventional solvent under conditions of conventional temperature and pressure. . For example, the extraction may be performed through various methods such as hot water extraction, cold extraction, reflux extraction, ultrasonic extraction, etc., but is not limited thereto.

1. Evaluation of Antioxidant Efficacy

1-1. Antioxidant effect of guava leaf extract and sweet potato extract mixture

In the same manner as in the manufacturing method described above with reference to FIG. 6, a mixed solution containing guava leaf and sweet potato extract as an active ingredient is prepared by dissolving the freeze-dried product of guava leaves and sweet potato in distilled water, centrifuging and filtering the supernatant. did.

Then, 100ul (microliter) of the mixed solution mixed with the guava leaf and sweet potato extract was reacted with 0.1mM DPPH (2,2-diphenyl-1-picrylhydrazyl) 50ul to prepare a solution for evaluating antioxidant efficacy .

Here, the preparation of the two solutions was reacted for 30 minutes in a light-shielded environment at room temperature, and the absorbance of the prepared solution was measured to evaluate the antioxidant efficacy according to the concentration.

At this time, Examples 1 to 5 provided for the antioxidant experiment are examples according to the ratio of the guava leaf extract and the sweet potato extract, and in more detail, Example 1 is that the concentration of the guava leaf extract is 0.1 for the mixture. % (w / v), Examples 2 to 4, the concentration of the guava leaf extract or sweet potato extract is 0.025% (w / v), 0.075% (w / v), 0.05% (w / v), and in Example 5, the concentration of the sweet potato extract was prepared to be 0.1% (w/v) with respect to the mixture.

Table 1 is a table showing the concentrations of the guava leaf extract and sweet potato extract of Examples 1 to 5 prepared for the antioxidant efficacy experiment.

mixing ratio Example Guava Leaf Extract (% (w/v)) Sweet Potato Extract (%(w/v)) Concentration sum (%(w/v)) Example 1 0.1 - 0.1 Example 2 0.075 0.025 0.1 Example 3 0.05 0.05 0.1 Example 4 0.025 0.075 0.1 Example 5 - 0.1 0.1

1 is a graph showing the antioxidant efficacy effect of a mixture according to the present invention, and a solution obtained by reacting the mixed solution with a DPPH (2,2-diphenyl-1-picrylhydrazyl) solution was subjected to a microplate photometer. The absorbance was measured using a reader (AMR100, Allsheng, Hangzhou).

Here, DPPH (2,2-diphenyl-1-picrylhydrazyl) is a stable free radical and is known to be used quickly and conveniently to evaluate antioxidant activity.

At this time, the wavelength of the light of the microplate reader (AMR100, Allsheng, Hangzhou) was set to 540 nm and the absorbance was measured. 100 ppm) was used, and for the antioxidant experiment, it was reacted with a DPPH solution as described above. In the antioxidant experiment, in Comparative Example 1, distilled water was used, and in Comparative Example 2, the positive control group was used.

In this antioxidant experiment, the absorbance of each sample was measured three times to represent the average value, and the value corresponding to the y-axis is the final corrected value (Ratio to Control), and the lower the value, the higher the antioxidant efficacy is expressed.

Here, to explain the calculation process of the final correction value, first, the correction value is calculated by subtracting the absorbance of each sample from the initial absorbance measured at 540 nm, and then, after converting the correction value of the measured distilled water to 1, It is a value obtained by converting each correction value based on the distilled water correction value 1.

1 is a graph showing the absorbance for confirming the antioxidant effect of a mixture of a guava leaf extract and a sweet potato extract according to the present invention.

As shown in FIG. 1 , as a result of an antioxidant experiment of a mixture of guava leaf extract and sweet potato extract, it was observed that the antioxidant effect was high in the order of Example 4, Example 1, Example 3, Example 2, and Example 5.

That is, in the case of the antioxidant effect, it was confirmed that the highest antioxidant effect can be expressed when the ratio of the guava leaf extract and the sweet potato extract is 1:3, which is the case of Example 4.

In particular, in the case of Example 4 and Example 1, the final correction value was lower than that of ascorbic acid, which is Comparative Example 2, and it was observed to have an excellent antioxidant effect than Comparative Example 2, which is known to have excellent antioxidant efficacy. .

1-2. Antioxidant effect of guava leaf extract

Next, in order to examine the antioxidant effect when the guava leaf extract was used alone, in the same manner as in the above-mentioned mixture preparation method, the freeze-dried guava leaf was dissolved in distilled water, and then centrifuged with a centrifuge to separate the supernatant. A solution containing guava leaf extract was prepared by filtration, and the solution necessary for the experiment was obtained by reacting with 50ul of DPPH in a light-shielding environment for 30 minutes.

Here, the ratio of the guava leaf extract is the same as the above-described sweet potato extract concentration, 0.00625% (w / v), 0.0125% (w / v), 0.025% (w / v), 0.05% (w / v) and 0.1 Examples 6 to 10 different in % (w/v) were used, and absorbance was measured at 540 nm three times each to show an average value, and Comparative Example 1 and Comparative Example 2 described above were used in the same manner.

In addition, since the process of deriving the final correction value is the same as the experimental process of the above-described mixture, a detailed description will be omitted.

Table 2 is a table showing the concentration of the guava leaf extract of Examples 6 to 10 prepared for the antioxidant efficacy experiment.

Example Guava Leaf Extract Example 6 0.00625% (w/v) Example 7 0.0125% (w/v) Example 8 0.025% (w/v) Example 9 0.05% (w/v) Example 10 0.1% (w/v)

Table 3 is a table showing the absorbance, self-absorbance, average correction value and final correction value of Comparative Examples 1 to 2 and Examples 6 to 9 prepared for the antioxidant efficacy experiment.

absorbance self absorbance Correction value average final correction value Comparative Example 1 0.031/0.034/0.121 0.047 0.082 1.0 Example 6 0.083/0.078/0.094 0.056 0.029 0.355 Example 7 0.088/0.085/0.095 0.057 0.032 0.396 Example 8 0.083/0.084/0.086 0.058 0.026 0.322 Example 9 0.092/0.083/0.088 0.061 0.027 0.327 Example 10 0.099/0.087/0.097 0.065 0.029 0.359 Comparative Example 2 0.084/0.091/0.083 0.050 0.036 0.441

2 is a graph showing the absorbance for confirming the antioxidant effect of the guava leaf extract according to the present invention.

As shown in FIG. 2 , as a result of the experiment, it was observed that the antioxidant effect was high in the order of Example 8, Example 9, Example 6, Example 10, and Example 7. That is, when the guava leaf extract was used alone, it was observed that the highest antioxidant effect could be expressed when the concentration of the guava leaf extract was 0.025% (w/v).

In addition, the final corrected value of each sample was observed to have a lower absorbance than ascorbic acid in Comparative Example 2, confirming that the guava leaf extract had a superior antioxidant effect than ascorbic acid, a positive control.

1-3. Antioxidant effect of sweet potato extract

And, in order to examine the antioxidant effect when the sweet potato extract was used alone, in the same manner as the above-described method for preparing a mixture of guava leaf extract and sweet potato extract, the dried sweet potato was dissolved in distilled water and then centrifuged with a centrifuge. The supernatant was filtered to prepare a solution containing sweet potato extract, and the solution required for the experiment was obtained by reacting with 50ul of DPPH solution in a light-shielding environment for 30 minutes.

Here, the concentration of the sweet potato extract is 0.00625% (w/v), 0.0125% (w/v), 0.025% (w/v), 0.05% (w/v) and 0.1% (w/v) for the mixture. Examples 11 to 15 prepared differently were used, and the same as the above-described antioxidant experiment process, detailed description will be omitted.

Table 4 is a table showing the concentration of the sweet potato extract of Examples 11 to 15 prepared for the antioxidant efficacy experiment.

Example Sweet Potato Extract Example 11 0.00625% (w/v) Example 12 0.0125% (w/v) Example 13 0.025% (w/v) Example 14 0.05% (w/v) Example 15 0.1% (w/v)

3 is a graph showing the absorbance for confirming the antioxidant effect of the sweet potato extract according to the present invention.

As shown in FIG. 3 , as a result of the experiment, it was observed that the antioxidant effect was high in the order of Example 15, Example 14, Example 13, Example 12, and Example 11. That is, when the sweet potato extract was used alone, the highest antioxidant effect could be expressed when the concentration of the sweet potato extract was 0.1% (w/v) with respect to the mixture, and the higher the concentration of the sweet potato extract, the higher the antioxidant effect. was observed to be

In particular, in the case of Example 15, it was confirmed that it has an antioxidant effect similar to that of Comparative Example 2.

Therefore, to summarize the above-mentioned antioxidant test results, it was verified that when the guava leaf extract was used alone, it had a higher antioxidant effect than when the sweet potato extract was used alone, and in the case of the mixture of the guava leaf extract and the sweet potato extract , it was confirmed that the concentration of guava leaf extract was 0.025% (w/v) and the concentration of sweet potato extract was 0.075% (w/v), which had the best antioxidant effect.

2. Whitening efficacy evaluation (tyrosinase (tyrosinase) activity inhibitory ability evaluation)

2-1. Confirmation of tyrosinase activity inhibitory effect of a mixture containing guava leaf extract and sweet potato extract

Tyrosinase (tyrosinase) refers to an enzyme that plays an important role in forming melanin from L-DOPA, and when the activity of tyrosinase is inhibited, a whitening effect can be expected.

In more detail, tyrosinase, which plays a role as a key enzyme in the production of melanin, is a metal protein (Etalloprotein) containing copper ions, and L-tyrosinase (L-tyrosine), one of the amino acids, is used as a substrate. It is converted into L-DOPA (3,4-dihydroxyphenylalanine), which is oxidized again to be converted to L-DOPA-quinone, and thus converted L-DOPA-quinone is MITF (Microphthalmiaassociated ranscriotion factor) and PLC (phospholipase C), TRP -1 (tyrosinase related protein-1), and TRP-2 (tyrosinase related protein-2) is synthesized by other proteins such as melanin.

The present invention is a mixture capable of providing a whitening effect by reducing tyrosinase function as well as antioxidant efficacy. In order to confirm this, the tyrosinase inhibitory effect of a mixture containing guava leaf extract and sweet potato extract was measured, For the experiment, the experiment was carried out as follows.

First, tyrosinase activity inhibition was evaluated according to 'Guideline I for Efficacy Evaluation of Functional Cosmetics (2015 Ministry of Food and Drug Safety)'.

And, in order to prepare a solution for evaluation of tyrosinase activity inhibition, first, guava leaves and sweet potato freeze-dried products were respectively dissolved in distilled water, and the concentration between the two extracts was the concentration of the mixture of the above-described antioxidant effect experiment (Table 1). Examples 16 to 20, the same as those of , were used.

Then, Examples 16 to 20, which are mixtures of prepared guava leaf extract and sweet potato extract of different concentrations, were mixed with the reaction solution and reacted, where the reaction solution was 10ul phosphate buffer (pH 6.5), 0.1M ) and 110ul of mushroom tyrosinase solution (mushroom tyrosinase, 1500U/ml) 20 μl).

After mixing the reaction solution, 20ul of a tyrosine solution (tyrosine, 1.5mM) was mixed and reacted at 37°C for 15 minutes to prepare a final solution for measuring tyrosinase activity inhibition.

Table 5 is a table showing the concentrations of the guava leaf extract and sweet potato extract of Examples 16 to 20 prepared for the tyrosinase activity inhibition test.

mixing ratio Example Guava Leaf Extract (% (w/v)) Sweet Potato Extract (%(w/v)) Concentration sum (%(w/v)) Example 16 0.1 - 0.1 Example 17 0.075 0.025 0.1 Example 18 0.05 0.05 0.1 Example 19 0.025 0.075 0.1 Example 20 - 0.1 0.1

In addition, in the experiment, distilled water was used as Comparative Example 3, a positive control group was used as Comparative Example 4, and arbutin (200 μM), which is known to have excellent whitening efficacy, was used as the positive control group. In addition, Comparative Examples 3 and 4 were reacted with the above-described reaction solution for the tyrosinase activity inhibition test.

And, in the experiment for verifying the whitening effect by confirming the ability to inhibit tyrosinase activity, the lower the final correction value, the better the whitening effect.

4 is a graph showing the absorbance for confirming the whitening effect of a mixture of a guava leaf extract and a sweet potato extract according to the present invention.

As shown in FIG. 4 , as a result of the experiment, it was observed that the whitening effect was high in the order of Example 18, Example 19, Example 17, Example 20, and Example 16.

That is, the whitening effect of the composition containing a mixture of guava leaf extract and sweet potato extract as an active ingredient was 0.05% (w/v) in the concentration of the guava leaf extract and 0.05% (w/v) in the mixture, and the concentration of the sweet potato extract was 0.05% ( w/v), it was confirmed to have the best whitening performance.

That is, when the ratio of guava leaf extract and sweet potato extract was 1:1, it was confirmed that the best whitening performance was obtained.

3. Assessment of cytoprotective efficacy

Another effect of the composition using the guava leaf extract and the sweet potato extract according to the present invention is to have a cell protective effect.

More specifically, in the present invention, the fact that it has a cytotoxic effect by inhibiting CCD-986SK cytotoxicity, which is one of human skin cells, has been identified, and the human skin cells may be CCD-986SK cells, but are limited thereto. It is not, and it can be used without limitation if it corresponds to the type of human skin cells.

Here, the CCD-986SK cells are a type of human skin fibroblast cells, and have the form of fibroblasts and have a characteristic of showing a pattern of growth in a monolayer.

3-1. Cell protective effect of guava leaf extract

In order to test the cytoprotective effect of the guava leaf extract of the present invention, as described above, a guava leaf solution was prepared by melting freeze-dried guava leaves in distilled water, and at this time, the concentration of guava leaves was 0.01% (w/ v), 0.05% (w/v), and 0.1% (w/v) solutions (Examples 21 to 23) were used.

Then, after immersion in CCD-986SK cells for 24 hours, 25ul of the supernatant was filtered to obtain a separation solution, and then, it was mixed with 25ul of the reaction solution and reacted at 37 degrees Celsius for 30 minutes to measure the cell protection efficacy. was secured.

Here, the LDH cytotoxicity assay kit, cayman (USA) was used as the reaction solution, and absorbance was measured at 492 nm using a microplate reader (AMR100, Allsheng, Hangzhou).

Table 6 is a table showing the concentrations of the guava leaf extract and sweet potato extract of Examples 21 to 26 prepared for the cell activity experiment.

mixing ratio Example Guava Leaf Extract (% (w/v)) Sweet Potato Extract (%(w/v)) Example 21 0.01 - Example 22 0.05 - Example 23 0.1 - Example 24 - 0.01 Example 25 - 0.05 Example 26 - 0.1

5 is a graph showing the absorbance for confirming the cytoprotective effect of the guava leaf extract and the sweet potato extract according to the cell protection experiment of the present invention, and the calculation method of the final correction value, which is the y-axis, is as described above, and in Comparative Example 5 Distilled water was used.

As shown in FIG. 5 , looking at the experimental results, the cytoprotective effect of the guava leaf extract was observed to be high in the order of Example 21, Example 22, and Example 23.

That is, when the guava leaf extract was used alone, when the concentration of the guava leaf extract was 0.01% (w/v) with respect to the mixture, it was observed that the highest cytoprotective effect was exhibited.

3-2. Cell protective effect of sweet potato leaf extract

In order to test the cytoprotective effect of the sweet potato extract of the present invention, as shown in Table 6, Examples 24 to 26 were used, which were prepared with a guava leaf extract solution and It was prepared using the same method and concentration.

As shown in FIG. 5 , looking at the experimental results, it was observed that the cytoprotective effect of the sweet potato extract was high in the order of Example 26, Example 25, and Example 24.

That is, when the sweet potato extract was used alone, when the sweet potato extract was 0.1% (w/v), it could be observed that the highest cytoprotective effect was exhibited, and it was found that the cytoprotective effect was higher than the above-described guava leaf extract. observed.

According to the above results, when a mixture of a guava leaf extract and a sweet potato extract according to the present invention or each of a guava leaf extract and a sweet potato extract was used alone, the antioxidant, whitening and cell protection effects were excellent, and it was used as a cosmetic composition to have an excellent effect , and in particular, it was observed that the antioxidant effect was superior to that of the conventionally widely used positive control group.

As described above, the present invention has been described with reference to the embodiments shown in the drawings, but this is only for explaining the invention, and various modifications or equivalents from the detailed description of the invention to those of ordinary skill in the art to which the present invention pertains It will be appreciated that one embodiment is possible.

Therefore, the true scope of the present invention should be determined by the technical spirit of the claims.

Claims (7)

A cosmetic composition comprising a mixture of guava leaf extract (Psidium guajava L.) and sweet potato extract (Ipomoea batatas) as an active ingredient. The method of claim 1,
The guava leaf extract or the sweet potato extract is a cosmetic composition, characterized in that the concentration of 0.01% (w / v) to 0.09% (w / v) with respect to the mixture. 3. The method of claim 2,
The guava leaf extract is at a concentration of 0.025% (w/v) with respect to the mixture, and the sweet potato extract is at a concentration of 0.075% (w/v) with respect to the mixture. The method of claim 1,
The mixture is a cosmetic composition, characterized in that the form of any one selected from a lotion, a nutritional lotion, a nourishing cream, a massage cream, a pack, an emulsified foundation, and a nutritional essence. A freeze-dried product preparation step of grinding the freeze-dried product of guava leaves and the freeze-dried product of sweet potato;
A mixed solution preparation step of dissolving the pulverized freeze-dried product in distilled water at a certain ratio to prepare a mixed solution;
After the step of preparing the mixed solution, a centrifugation step of centrifuging; and
After the centrifugation step, a mixture extraction step of extracting the mixture by filtering the supernatant; Cosmetic composition manufacturing method comprising a. 6. The method of claim 5,
The mixture produced in the mixture extraction step is,
A method for manufacturing a cosmetic composition comprising guava leaf extract and sweet potato extract as active ingredients. 6. The method of claim 5,
The mixed solution preparation step,
A method for producing a cosmetic composition, characterized in that the sum of the concentrations of the guava leaf extract and the sweet potato extract is 0.1% (w/v).

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