KR20210075424A - Composition for attenuating inflammation containing complex natural hub extracts - Google Patents

Abstract

The present invention relates to a composition for alleviating inflammation containing complex natural components. More specifically, the present invention relates to a composition for alleviating inflammation which contains a liquid natural extract of Eucalyptus globulus and Melaleuca alternifolia, thereby having an effect of alleviating inflammation such as rhinitis, allergic rhinitis, and sinus cold. The composition of the present invention can be prepared in a form of an intranasal spray and can be applied as a nasal cleansing composition, and when the composition for alleviating inflammation is used as a pharmaceutical composition containing an active component for relieving and treating respiratory inflammation, it is possible to relieve and treat rhinitis, allergic rhinitis, asthma, or sinus cold.

Description

Composition for attenuating inflammation containing complex natural hub extracts

The present invention relates to a composition for relieving inflammation containing complex natural ingredients, and more particularly, to a composition containing a liquid natural extract of eucalyptus and tea tree, particularly effective in improving inflammation such as rhinitis, allergic rhinitis, and nasal cold. It relates to a composition for alleviating inflammation with

As life becomes more complicated and various convenience facilities are developed, the atmospheric environment is constantly changing, and various respiratory diseases caused by fine dust are increasing significantly. Among them, diseases such as rhinitis, allergic rhinitis, cold, and asthma are a direct problem due to the atmospheric environment.

The common cause of asthma and allergic rhinitis is hypersensitivity to a specific substance, an allergy (antigen). Allergic rhinitis is a disease that causes the four major symptoms of sneezing, runny nose, itching, and nasal congestion due to loss of normal nasal function.

In the case of an asthma attack, it is called a morning dip, and the patient mainly suffers from sleep disturbance due to coughing and shortness of breath, etc. due to a decrease in exhaled air flow around dawn. In severe cases, it is difficult to find an emergency room at night due to severe breathing difficulties. this will happen

Therefore, in order to prevent breathing difficulties through the bronchial tubes due to asthma at dawn, apply it right before sleep to reduce inflammation so that the bronchial or nose breathing can be maintained well for several hours, and to prevent the phenomenon of blocking the breathing of the bronchial tubes due to swelling or congestion in the nasal passages. Relief is of paramount importance.

Allergic rhinitis is caused by pollen in the case of seasonality, so it is called hay fever. In other cases, house dust, mites, and fungi are the main antigens. However, other than that, it can be said that stress stimulation is significantly involved in inducing allergic rhinitis.

As a treatment for relieving asthma and rhinitis, oral, inhalant, tulobuterol asthma patch and products using aroma essential oil as an alternative therapy are being marketed.

Among these, oral preparations may cause side effects of gastrointestinal or hepatic disorders due to drug toxicity, and the drug itself may act as an antigen, resulting in asthma and rhinitis.

In addition, it is difficult to administer to infants, children, or unconscious patients, and in the case of asthma that occurs suddenly at night, it is difficult to find an emergency room at night or to receive an oral prescription for asthma treatment, which is a prescription drug, from a late-night pharmacy. can follow

Inhalation formulations have fewer side effects than oral formulations, but they are difficult to apply to infants, children, and unconscious patients. Frequent inhalation may cause the mist to irritate the nasal mucosa and cause sneezing or additional inflammation of the nasal mucosa due to air pressure during inhalation.

Aromatherapy, which has recently emerged as an alternative therapy, is a method of treating diseases of the body using fine particles emitted from plants. It provides various therapeutic effects by penetrating natural oils with healing effects into the body through our nose or skin. It is a treatment that brings The properties of aroma essential oil that have been proven so far are composed of organic substances including alcohol, aldehyde, ester, ketone, and oxide, and these ingredients are anti-infection, anti-fungal, anti-histamine, anti-allergy, anti-viral, expectorant, antitussive, It has sedative and antispasmodic effects, but its inconvenient use is pointed out as a problem.

Therefore, all of the prior arts have a problem in that they cannot show an appropriate therapeutic effect depending on the age of the asthma and rhinitis patient, the patient's condition, treatment time, surrounding environment, and the patient's sleep or activity state.

Korean Patent Publication No. 10-2013-0060092 (asthma and rhinitis relief hydrogel patch composition having a multi-drug diffusion control layer structure of aroma essential oil and natural extract) as a composition for relieving inflammation, particularly rhinitis, using conventional natural ingredients, Korea Patent Publication No. 10-2018-0032644 (nose composition), Korean Patent Publication No. 10-2006-0015821 (rhinitis treatment containing herbs), Japanese Patent Publication No. 2003-286178 (extract of eucalyptus lemon as an active ingredient) anti-allergic agents and foods containing them) have been proposed. In addition, a number of compositions for alleviating allergy or inflammation using various natural ingredients have been proposed.

Such existing technologies also have limitations in relieving inflammation such as rhinitis as described above, and there are also problems in that they are inconvenient to use.

Therefore, the composition for the treatment or alleviation of respiratory inflammation such as rhinitis still has a very urgent problem with a lot of room for development,

Korean Patent Publication No. 10-2013-0060092 Korean Patent Publication No. 10-2018-0032644 Korean Patent Publication No. 10-2006-0015821 Japanese Patent Laid-Open No. 2003-286178

It is a task to solve the above problems in the conventional composition for alleviating inflammation, and to construct and present a new complex natural extract that, unlike the prior art, has an anti-inflammatory effect such as rhinitis and exhibits a convenient effect for use.

Accordingly, an object of the present invention is to provide a composition for alleviating inflammation that is effective in relieving inflammation, such as rhinitis, by comprising a specific complex natural extract that cannot be constructed in the past.

Another object of the present invention is to provide a nasal cleansing composition or pharmaceutical composition comprising herbal care water that is composed of a specific complex natural extract and is effective for rhinitis, allergic rhinitis, asthma, and the like.

In order to solve the above problems of the present invention, the present invention provides a composition for relieving inflammation, comprising a complex natural extract consisting of eucalyptus and tea tree as an active ingredient.

According to a preferred embodiment of the present invention, a complex natural extract consisting of 10-50 parts by weight of eucalyptus and 10-50 parts by weight of tea tree can be preferably configured as a composition for alleviating inflammation.

In addition, the present invention provides a nasal cleansing composition comprising a composition for alleviating inflammation.

In addition, the present invention provides a pharmaceutical composition comprising a composition for alleviating inflammation.

According to a preferred embodiment of the present invention, the composition for alleviating inflammation may additionally contain one or more selected from eosinthia, rosemary, nasturtium, chamomile, and aroma oil.

The present invention is composed of a complex natural extract by selecting a specific ingredient that has been previously used as a natural ingredient in a cosmetic composition, and exhibits a synergistic effect on pain relief and inflammation relief due to the complex use of these specific ingredients.

In particular, the composition of the present invention exhibits a remarkable effect in improving respiratory-related inflammation such as rhinitis, and in particular, exhibits an excellent synergistic effect in rhinitis, allergic rhinitis, asthma, and the like. In particular, when used by spraying into the nasal cavity, the effect is excellent.

Therefore, the composition for alleviating inflammation according to the present invention is very useful as a pharmaceutical composition for the treatment and prevention of inflammatory respiratory diseases, specifically, rhinitis, allergic rhinitis, asthma, nasal cold, and the like.

Hereinafter, the present invention will be described in more detail as one embodiment as follows.

The present invention is a composition for alleviating inflammation, characterized in that it contains a complex natural extract.

Especially. The complex natural extract of the present invention was constructed based on the results of a new study that found that there was a surprising effect that could not be predicted due to a synergistic effect when a specific ingredient was selectively mixed among ingredients previously used as cosmetics or similar functional ingredients.

The complex natural extract selectively used in the present invention is a mixed component of eucalyptus and tea tree.

According to a preferred embodiment of the present invention, the synergistic effect of these two mixed components has a very surprising effect of alleviating inflammation, unlike mixtures of existing components.

According to a preferred embodiment of the present invention, in the content range consisting of 10-50 parts by weight of eucalyptus and 10-50 parts by weight of tea tree, a particularly remarkable synergistic effect is exhibited. More preferably, 10-40 parts by weight of calyptus and 10-40 parts by weight of tea tree, and most preferably, 20-40 parts by weight of calyptus and 20-40 parts by weight of tea tree may be mixed and used.

If the content of the specific component among these two complex natural extracts is small or large, there is a problem in that it is difficult to expect a remarkable synergistic effect.

In particular, according to a preferred embodiment of the present invention, when eucalyptus is used in too little amount, the anti-inflammatory effect is lowered, and it is difficult to expect a synergistic effect. It is difficult to expect a synergistic effect.

According to the present invention, there are many herbal medicines effective in relieving respiratory-related inflammation among natural ingredients. However, among them, it was confirmed that the two complex natural extracts selectively used in the present invention exert a special synergistic effect in relieving inflammation such as rhinitis. This is considered to be an effect obtained not by simply mixing two natural ingredients, but by selective mixing of specific ingredients.

According to a preferred embodiment of the present invention, the complex natural extract of the above composition essentially exhibits an excellent effect in relieving inflammation as a respiratory disease, and is particularly effective in rhinitis, allergic rhinitis, asthma, nasal cold, etc. through intranasal administration. indicates

Eucalyptus used by a specific selection in the present invention is effective in preventing bronchial and respiratory diseases such as rhinitis or bronchitis by removing bacteria in the bronchial and respiratory tract with a strong sterilizing action and discharging inflammation to the outside. In particular, aromatherapy using eucalyptus oil is very effective in treating respiratory diseases and pain.

Eucalyptus has excellent anti-inflammatory action, and it is also effective in alleviating arthritis, which causes pain due to the deposition of inflammation in the joints. In particular, as it relieves pain, it is very helpful in relieving pain such as muscle pain, joint pain, and sprains.

In addition, eucalyptus is known to have a powerful sedative effect, so it relaxes sensitive nerves and helps relieve stress. In fact, it is effective in removing odors and odors in the room, so if you use eucalyptus as an air freshener and use it in a closet or shoe cabinet, the odor removal and sterilization effect is great.

In addition, eucalyptus has a strong sterilization effect and is effective in treating wounds by neutralizing the poison caused by insect bites.

In particular, eucalyptus has a strong antibacterial action, which helps to regenerate the skin and is effective in alleviating rashes and various skin problems, and is also effective in alleviating blemishes and acne.

In addition, the tea tree used in the present invention is often used by distillation in the form of oil, and it has been proven to have antibacterial and antifungal properties. It is said that during World War II, it was given to soldiers in the tropics as a treatment for skin wounds and was also used to treat wounds.

Since tea tree contains a lot of antioxidants, tea tree oil can be used as a disinfectant because it has excellent antibacterial and disinfecting effects.

As such, because of the antibacterial and antifungal effects of tea tree, it is used in various forms in the form of drugs or cosmetics, such as wound disinfectants, acne soothing agents, athlete's foot treatment, respiratory disease treatment, and the like. However, it is recommended to dilute the undiluted solution before use because it has strong irritation.

In addition, tea tree is good for acne of the skin, especially purulent acne, and has the advantage of being able to use it regardless of oily or dry skin, and it has an excellent therapeutic effect especially in the case of eczema. This is thought to be due to the anti-inflammatory and antibacterial effects.

The present invention confirmed for the first time the fact that the complex prescription of these two components exerts an unpredictable effect on inflammation relief such as rhinitis.

The complex natural extract containing eucalyptus and tea tree according to the present invention as described above may be used in leaves, flowers, fruits, stems, roots, etc., in various forms such as oils, extracts, powders, liquids, or mixtures thereof It can be applied as an extract of

According to a preferred embodiment of the present invention, at least one or more of these components may include a liquid or oil form. In addition, some may be mixed and used in powder form. Preferably, it is used as a liquid, and it is good to use a very small amount in the form of an extract.

According to a preferred embodiment of the present invention, the composition for alleviating inflammation of the present invention may additionally contain one or more selected from eosinthia, rosemary, nasturtium, chemomile, and aroma oil. It may contain one or more water extracts or oils selected from among them.

According to the present invention, when these ingredients are applied in the form of a water extract, it can be called herbal care water.

Aroma oil in the present invention, for example, jojoba oil, sweet almond oil, hazelnut oil, evening prime rose oil, olive oil, apricot kernel oil, avocado oil, grapeseed oil, rosehip oil, etc. may be used in a mixture of one or more.

One or more natural ingredients that may be additionally used in the present invention may be used in an amount of 1-50 parts by weight. More preferably, each component may be used in an amount of 0.1-10 parts by weight, respectively.

In particular, according to a preferred embodiment of the present invention, it is preferable to additionally contain 10-40 parts by weight of a mixed water extract of eoseongcho, rosemary, and chrysanthemum.

In addition, according to a preferred embodiment of the present invention, chemomile, aromatic oil, or a mixture thereof may be used in an amount of 0.1-5 parts by weight, respectively.

According to a preferred embodiment of the present invention, in the case of such a natural extract, the composition for alleviating respiratory inflammatory diseases such as rhinitis is preferably applied as an external preparation for the skin and can be used as a simple nasal cleanser. These nasal cleansers can be applied by intranasal spraying. In this case, it is good for maintaining cleanliness caused by fine dust, etc., and can alleviate, prevent, or suppress the occurrence of rhinitis and a cold.

As a preferred embodiment of the present invention, the composition for alleviating inflammation can be used as a pharmaceutical composition containing the composition as an active ingredient for alleviation or treatment of rhinitis, allergic rhinitis, asthma, and nasal cold.

According to a preferred embodiment of the present invention, such a pharmaceutical composition can be used by spraying into the nasal cavity.

According to a preferred embodiment of the present invention, the complex natural extract of the present invention can be specifically applied as a nasal rinse or a pharmaceutical composition by spraying it several times in the nasal cavity at 1-1,000 mg/ml.

This form can be prepared by composing 1-200 mg/ml when applied as a nasal cleanser composition to be applied to the skin.

According to a preferred embodiment of the present invention, the pharmaceutical composition containing the complex natural extract according to the present invention as an active ingredient is, for example, 50mg/ml, 100mg/ml. It can be prepared and applied in various contents such as 200mg/ml, 300mg/ml.

In addition, the pharmaceutical composition according to the present invention may be used, for example, in the form of a cooling lotion for pain relief and inflammation relief, preferably by spraying it frequently at 400 mg/ml, 500 mg/ml or 1,000 mg/ml several times a day.

According to a preferred embodiment of the present invention, when the composition for alleviating inflammation exceeds the lower limit of the content range, the effect is significantly reduced, and when it exceeds the upper limit, it is difficult to prepare a spray spray composition or formulation, or it is expected compared to the amount added. Since the effect is not greatly improved, it is not preferable from an economic point of view.

As described above, according to the present invention, the composition for alleviating inflammation as described above can be used for medieval relief of inflammatory diseases of the respiratory system, such as rhinitis, and intranasal injection for alleviating or treating rhinitis, allergic rhinitis, asthma, nasal cold, etc. zero could be applied.

Hereinafter, the present invention will be described in more detail based on Examples, but the present invention is not limited by the Examples.

Example 1-5, Comparative Example 1-3

In order to construct a sprayable liquid-type composition for alleviating inflammation, each component as shown in Table 1 below was mixed in the corresponding content and stirred sufficiently at room temperature to prepare a composition.

The content of each ingredient used herein is in parts by weight.

Example One 2 3 4 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 eucalyptus 25 40 10 20 20 50 - 50 tea tree 25 10 40 20 20 - 50 - Eoseongcho - - - 7 7 - - 7 Rosemary - - - 7 7 - - 7 nasturtium flower - - - 7 7 - - 7 chamomile - - - - 0.5 - - - aroma oil - - - - 2 - - 2 * Eucalyptus water and tea tree use liquid extracts.

Experimental Example 1; NO inhibitory effect

The inhibitory effect on NO, an inflammatory factor involved in the inflammatory reaction, was observed for each composition sample of Examples and Comparative Examples.

In RAW 264.7 cell line, LPS and samples (each composition was dissolved in 0.5% methyl cellulose solution) were treated at 50 μg/ml and 100 μg/ml, respectively, and cultured for 20 hours. Then, 100 μl of the culture solution was placed in a 96-well plate. , 50 μl of Griess reagent was added, followed by reaction at room temperature for 10 minutes, and then absorbance was measured at 540 nm with an ELISA reader.

The results are as shown in Table 2 below.

sample to be used Content (㎍/ml) NO inhibition rate (%) Example 1 50 17.3 100 23.7 implementation 4 50 17.6 100 24.9 Comparative Example 1 50 9.6 100 15.2 Comparative Example 3 50 10.1 100 15.8

As a result of the above experiment, it was confirmed that the composition of Example exhibits a significantly superior NO inhibitory effect compared to the composition of Comparative Example, and the effect shows that it is excellent in the effect involved in the inflammatory reaction.

Experimental Example 2: Rhinitis improvement effect

For each sample of Examples and Comparative Examples, an experiment was conducted to confirm the physical properties and preferences in the use state.

The experiment was conducted on people with chronic rhinitis or asthma. 15 subjects with 9 rhinitis and 6 asthmatics were asked to use it 3-5 times a day for 5 days, wash their noses after going out, and quantify and record the quality according to each item.

The sample was evaluated for the relief of the runny nose, the relief of nasal congestion, the relief of respiratory distress, the recovery rate of rhinitis, the feeling of use, and the like, compared with the existing rhinitis treatment.

The evaluation score is set to 1-10, good quality is evaluated as 10, and poor quality is evaluated as 1, and the evaluation score is calculated by adding up all evaluation scores of the subject, obtaining the average value of 1, and then rounding or rounding the calculated value. As the final result, the final measurement result is summarized in Table 3 below.

Example runny nose relief nasal congestion relief respiratory distress relief rhinitis recovery speed feeling
(convenience) One 10 10 9 10 10 2 10 10 8 9 10 3 10 9 8 9 10 4 10 10 10 10 10 5 10 10 10 10 10 Comparative Example 1 5 5 6 6 7 Comparative Example 2 3 4 6 6 7 Comparative Example 3 5 6 6 6 6

Experimental Example 3: Histamine release inhibition test

CD34-positive cells were isolated from umbilical cord blood using a magnetic cell separation system (MACS; Miltenyi Bioteck). Medium for differentiating the isolated cells into mast cells (IL-6; 50 ng/ml, SCF; 80.5 ng/ml, Penicillin; 100 U/ml, Streptomycin; 100 μg/ml, FCS; MEM-α containing 10% ( GIBCOBRL (manufactured by GIBCOBRL)) to prepare human mast cells by culturing _{at 37°C in an atmosphere of 5% CO 2 .} Incidentally, the cells were used for the experiment when the medium was changed at a frequency of once a week, and the cells were 15 to 30 weeks old.

Immunoglobulin E (IgE) was added to the human mast cell suspension obtained as described above (final concentration; 1 µg/ml) and cultured for 18 hours. Tie rod (126 mmol/l NaCl, 4 mmol/l KCl, 0.64 mmol/l NaH ₂ PO ₄ , 1 mmol/l CaCl ₂ , 0.6 mmol/l MgCl ₂ , 0.1% glucose, 10 mmol/l HEPES, 0.03% BSA) After washing in , the cells were resuspended in a tie rod so that the number of cells was 1.25×10 ^{4 (cells/ml).}

400 μl of the cell suspension was dispensed into the Eppendorf tube and heated at 37° C. for 5 minutes. Then, 50 μl of tie rods were added to the sensitized and untreated groups, and the extract solution prepared from each sample extract at a predetermined concentration in the tie rods was added to the extract-added group. 50 μl was added. After heating at 37°C for 10 minutes, 50 μl of anti-IgE was added to the sensitized group and extracted addition group, and 50 μl of tie rod was added to the untreated group. After heating at 37° C. for 30 minutes, centrifugation (1500 rpm, 6 minutes, 4° C.) ), the supernatant was recovered, and the amount of histamine in the supernatant was measured by the post-column-derivatization method.

In addition, the histamine inhibition rate was calculated using the following formula, and the results are shown in Table 4 below.

[Equation]

Inhibition rate (%) = [1-(Sample administration group - untreated group) / (sensitized group - untreated group)] × 100

sample Extract concentration (g/ml) Inhibition rate (%) Example 1 10 ^-4 91±2 10 ^-5 90±5 10 ^-6 82±8 Example 4 10 ^-4 93±1 10 ^-5 91±4 10 ^-6 84±11 Comparative Example 1 10 ^-4 68±1 10 ^-5 57±7 10 ^-6 44±13 Comparative Example 3 10 ^-4 72±2 10 ^-5 62±6 10 ^-6 56±12

It was confirmed that the extracts of Examples have a significant inhibitory effect on degranulation from mast cells of type I allergic reaction in inhibiting the release of histamine from human mast cells compared to Comparative Examples. This means that it has an excellent effect on rhinitis, allergic rhinitis, and asthma.

From the above experimental results, it was confirmed that the composition of the present invention was effective in relieving inflammation, and in particular, it was confirmed that it exhibited a significantly superior effect compared to the comparative examples due to the synergistic effect of the two components used in Examples 1-3. .

Claims (8)

A composition for relieving inflammation containing a complex natural ingredient containing a complex natural extract consisting of eucalyptus and tea tree as an active ingredient.
The composition for relieving inflammation according to claim 1, containing a complex natural component consisting of 10-50 parts by weight of eucalyptus and 10-50 parts by weight of tea tree.
[Claim 2] The composition for relieving inflammation according to claim 1, further comprising at least one selected from the group consisting of seaweed, rosemary, nasturtium, chamomile, and aroma oil.
10-50 parts by weight of eucalyptus; and 10-50 parts by weight of tea tree; And 10-40 parts by weight of a mixed water extract of eosinthwort, rosemary, and rhododendron flower; A composition for relieving inflammation containing a complex natural component.
[Claim 5] The composition for relieving inflammation according to any one of claims 1 to 4, which contains a complex natural ingredient in a form applied as an intranasal spray.
A nasal cleansing composition comprising a composition for alleviating inflammation containing the complex natural ingredients according to any one of claims 1 to 4.
A pharmaceutical composition comprising the composition for alleviating inflammation containing the complex natural ingredients according to any one of claims 1 to 4 as an active ingredient for alleviating and treating respiratory inflammation.
The pharmaceutical composition according to claim 7, for alleviating and treating rhinitis, allergic rhinitis, asthma or a cold.