KR20210066737A - Cosmetic composition containing retinol and pyruvate - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising retinol and pyruvate as active ingredients. Since the cosmetic composition according to the present invention comprises retinol, sodium pyruvate, niacinamide, hyaluronic acid and hydrolyzed collagen in a specific content, although the cosmetic composition contains the retinol, the composition relieves skin dryness and skin inflammation caused by the retinol to have excellent effects of alleviating wrinkles, acne, psoriasis, ichthyosis and the like by the retinol.

Description

Cosmetic composition containing retinol and pyruvate

The present invention relates to a cosmetic composition containing retinol for improving wrinkles, acne, psoriasis, ichthyosis, etc. By including pyruvate together, it is excellent in the improvement effect of wrinkles, acne, psoriasis, ichthyosis, etc., while preventing the skin from drying out and suppressing the occurrence of skin inflammation, as well as retinol It relates to a cosmetic composition in which side effects are alleviated.

Retinol is a type of vitamin A, also called a pure vitamin. As a substance that plays an important role in maintaining the original function of the epidermal cells of the skin, it is specifically referred to as pure vitamin A as the chemical name of vitamin A1. It is present in the mucosal cells of the intestinal tract of animals and is abundantly contained in green and yellow plants. It is also transformed into the active form of retinoic acid, and is known to play an important role in maintaining the original function of epidermal cells. In addition, it promotes cell differentiation by expressing RNA among the cell nuclei present in skin cells, and promotes biosynthesis of collagen, a hard protein that exists as a fibrous solid between animal cells, and elastin, which is composed of elastic fibers, to create wrinkles. It is known to have the effect of reducing skin elasticity and increasing skin elasticity.

Retinol as a cosmetic composition has the effect of increasing collagen synthesis in the skin and promoting cell production by promoting turnover of the stratum corneum. Not only does it have the disadvantage of causing it, but it also has a problem in that retinol itself is very unstable, so that it is denatured or decomposed over time, resulting in a decrease in potency and thus the use of retinol in cosmetics is extremely limited.

Therefore, there have been many attempts to solve the above problems with retinol by adding other ingredients along with retinol to cosmetics. Cheonmundong extract (Korean Patent Application Laid-Open No. 10-2005-0121397), which inhibits the production of PGE2, an inflammation-inducing eicosanoid in the cyclooxygenase pathway, thereby suppressing inflammation and alleviating skin irritation. Watermelon endothelial extract that prevents irritation and inflammation and relieves sensitive skin (Korean Patent Publication No. 10-2005-0080480), Nanoliposome stabilized by enclosing a cypress extract (Korean Patent Publication No. 10-2008-0062224) ), mung bean extract that relieves skin irritation caused by retinol (Korean Patent Application Laid-Open No. 10-2003-0042527), and inhibits the growth of retinol and acne-forming strains and is an intercellular lipid component of the stratum corneum with excellent anti-inflammatory effect. Effectively reduce skin irritation, transdermal moisture loss and inflammatory cytokines increased by phytosphingosine and Yulpi extract (Korean Patent Publication No. 10-2000-0065305), which are important intermediates in ceramide biosynthesis, and retinoids Fatty acid or C10-40 isoalkyl acid cholesterol ester, phytostearyl / isostearyl / cetyl stearyl / behenyl dimer corresponding to the ligand of the peroxisome proliferator-activated receptor alpha that can be activated There was a lipid complex of dilinoleate and canola oil (Korean Patent Application Laid-Open No. 10-2010-0023246).

However, skin irritation caused by retinol does not appear in a single form, but includes irritation such as itching, stinging, and burning, and various inflammatory stimuli such as erythema and edema. Because the reaction appears, its usage is limited, and there was a problem that it was not enough to alleviate irritation simply by using one anti-inflammatory ingredient.

Therefore, by suppressing skin irritation by retinol, it prevents the skin from drying out and suppresses the occurrence of skin inflammation, while improving the effects of wrinkles, acne, psoriasis, ichthyosis, etc. There is a need for research on excellent cosmetic compositions.

Republic of Korea Patent Publication No. 10-2005-0121397 Republic of Korea Patent Publication No. 10-2005-0080480 Republic of Korea Patent Publication No. 10-2008-0062224 Republic of Korea Patent Publication No. 10-2003-0042527 Republic of Korea Patent Publication No. 10-2000-0065305 Republic of Korea Patent Publication No. 10-2010-0023246

Accordingly, an object of the present invention is to prevent skin from drying out by suppressing skin irritation caused by retinol by including pyruvate as an active ingredient in a cosmetic composition containing retinol, and suppressing the occurrence of skin inflammation, wrinkles , acne (acne), psoriasis (psoriasis), ichthyosis (ichthyosis), etc. to provide a more excellent cosmetic composition.

In order to achieve the above object, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol while containing retinol and pyruvate as active ingredients.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the retinol content is 0.01 to 0.5% by weight.

In addition, the present invention provides for the relief of skin irritation caused by retinol, wherein the pyruvate is selected from the group consisting of lithium pyruvate, sodium pyruvate, potassium pyruvate, magnesium pyruvate, calcium pyruvate, zinc pyruvate, manganese pyruvate, and mixtures thereof. It provides a cosmetic composition for

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the content of the pyruvate is 0.01% to 5% by weight based on the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the weight ratio of pyruvate to retinol is 1:10 to 1:100.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the cosmetic composition further comprises niacinamide, hyaluronic acid and hydrolyzed collagen.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the content of niacinamide is 0.1% to 2% by weight based on the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the content of the hyaluronic acid is 0.001% to 0.03% by weight based on the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the content of the hydrolyzed collagen is 0.001% to 0.03% by weight based on the total weight of the cosmetic composition.

In addition, the present invention, the cosmetic composition contains sodium pyruvate 0.03 to 3% by weight, niacinamide 0.5 to 1.5% by weight, hyaluronic acid 0.007 to 0.015% by weight, and hydrolyzed collagen 0.007 to 0.015% by weight based on the total weight of the cosmetic composition to provide a cosmetic composition for alleviating skin irritation caused by retinol.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the cosmetic composition has any one formulation selected from ointment, nutritional lotion, massage cream, pack, and lotion.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, which has the effect of more effectively improving skin wrinkles.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, which has an effect of preventing skin dryness.

In addition, the present invention provides a cosmetic composition for alleviating skin irritation caused by retinol, wherein the cosmetic composition has an effect of inhibiting skin inflammation.

According to the present invention, by including retinol and pyruvate as an active ingredient as a cosmetic composition as an active ingredient, without causing skin dryness and skin inflammation due to retinol, wrinkles, acne, and psoriasis , ichthyosis, and the like more effectively.

Specifically, according to the present invention, as a cosmetic composition, retinol, sodium pyruvate, niacinamide, hyaluronic acid and hydrolyzed collagen are included in a specific content, thereby alleviating the occurrence of skin dryness and skin inflammation caused by retinol, It has the effect of more effectively improving wrinkles, acne, psoriasis, and ichthyosis.

1 is a graph showing the lapse of time (0 hours, 24 hours, and 48 hours) between keratinocytes treated with sodium pyruvate of the present invention at 1 mM and 3 mM and keratinocytes not treated with sodium pyruvate (control group) It is a graph comparing cell viability by measuring the difference in absorbance at 450 nm and 600 nm.
2 is a graph comparing the cell viability of keratinocytes treated with sodium pyruvate of the present invention at a concentration of 3 mM (Example) and keratinocytes not treated with sodium pyruvate (control) after 48 hours.
3 shows keratinocytes treated with retinol and sodium pyruvate according to the present invention (Example), keratinocytes treated only with retinol (Comparative Example), and keratinocytes not treated with both retinol and sodium pyruvate (control group) It is a graph showing the relative mRNA expression levels of HAS2, CLDN1, and FLG.
4 shows K1 (Keratin) of keratinocytes untreated, retinoic acid (RA)-treated keratinocytes, retinol-treated keratinocytes, and keratinocytes treated with sodium pyruvate. 1), K10 (Keratin 10), FLG (Filaggrin), CXCL 1, CXCL 2, a graph showing the relative mRNA expression levels of CCL20.

Hereinafter, preferred embodiments of the present invention will be described in detail so that those of ordinary skill in the art to which the present invention pertains can easily practice the present invention.

The cosmetic composition according to the present invention is for alleviating skin irritation caused by retinol, and may include pyruvate as an active ingredient.

Although the cosmetic composition containing the pyruvate as an active ingredient contains retinol, which is known to cause skin irritation, it reduces the skin barrier function due to retinol and reduces dryness, thereby causing wrinkles, acne, and psoriasis. , shows an excellent effect of improving ichthyosis and the like.

The retinol is a type of vitamin A and plays an important role in maintaining the original function of the epidermal cells of the skin. Specifically, it promotes cell differentiation by expressing RNA from DNA among the cell nuclei present in skin cells, and promotes biosynthesis of collagen, a hard protein that exists as a fibrous solid between animal cells, and elastin, which is composed of elastic fibers. It has the effect of reducing wrinkles and increasing skin elasticity. In addition, the efficacy of retinol on the skin increases proportionally with the concentration up to about 6000 IU, and there is no significant difference at or above 6000 IU (S.Kang et al., J. Investigative Dermatology, 105, 549-556, 1995). ), the amount of retinol that can increase the efficacy of retinol while minimizing skin irritation is about 5000IU, maximizing the effect on the skin. However, even a cosmetic composition having a concentration of 2500 IU or more of retinol may cause great irritation to the skin when used.

Accordingly, an object of the present invention is to provide a cosmetic composition capable of alleviating skin irritation caused by retinol by including pyruvate in a specific amount together with retinol.

In the cosmetic composition according to the present invention, the content of the retinol as an active ingredient for improvement of wrinkles, acne, psoriasis, ichthyosis, etc. may be 0.01 wt% to 0.5 wt%, preferably Preferably, it may be 0.3 wt% to 0.5 wt%. When the content of the retinol is less than 0.01% by weight, the effect of improving wrinkles is somewhat weak, and when it exceeds 0.5% by weight, the effect is not proportional to the content, so it is uneconomical in terms of cost, so the content of the retinol is within the above range It is desirable to satisfy

In addition, the pyruvate may be selected from the group consisting of lithium pyruvate, sodium pyruvate, potassium pyruvate, magnesium pyruvate, calcium pyruvate, zinc pyruvate, manganese pyruvate, and mixtures thereof, preferably sodium pyruvate.

The content of the pyruvate may be 0.01% to 5% by weight, preferably 0.03% to 4% by weight, and more preferably 0.04% to 3% by weight based on the total weight of the cosmetic composition. can When the content of the pyruvate is less than the above range, the damage to skin cells exposed to ultraviolet light cannot be sufficiently suppressed, that is, the decrease in cell viability cannot be sufficiently suppressed. When the content of the pyruvate exceeds the above range, the skin Since there is a problem of cytotoxicity to cells, the content of the pyruvate preferably satisfies the above range.

The weight ratio of pyruvate to retinol may be 1:10 to 1:100, preferably 3:50 to 1:20, and more preferably 3:10 to 1:10. When the weight ratio of pyruvate to retinol is less than the above range, skin irritation caused by retinol cannot be sufficiently alleviated. When the weight ratio of pyruvate to retinol exceeds the above range, Since there is a problem in showing cytotoxicity to the retinol, the weight ratio of pyruvate to retinol preferably satisfies the above range.

The cosmetic composition for alleviating skin irritation caused by retinol according to the present invention may further include niacinamide, hyaluronic acid and hydrolyzed collagen in addition to the pyruvate.

The niacinamide, as a vitamin B3 component, inhibits the movement of melanin generated in the skin to the skin surface, thereby suppressing pigmentation and improving skin tone. In particular, when used together with the pyruvate salt used in the present invention, it not only relieves skin irritation caused by retinol, but also acts in each stage of skin whitening to show a synergistic effect, so it is recommended to use it together with the pyruvate salt of the present invention. desirable.

In addition, the content of niacinamide may be 0.1 wt% to 2 wt%, preferably 0.5 wt% to 1.5 wt%, more preferably 0.8 wt% to 1 wt%, based on the total weight of the cosmetic composition It can be %. When the content of the niacinamide is less than the above range, the movement of melanin generated in the skin to the skin surface cannot be sufficiently inhibited, and when the content of the niacinamide exceeds the above range, skin irritation may occur. The content of the amide preferably satisfies the above range.

In addition, the hyaluronic acid is a kind of mucoda saccharides and is present in the connective tissue of the dermal layer of the human body and plays an important role in maintaining moisture. The content of the hyaluronic acid may be 0.001% by weight to 0.03% by weight based on the total weight of the cosmetic composition, preferably 0.005% by weight to 0.015% by weight, more preferably 0.007% by weight to 0.012% by weight can When the content of the hyaluronic acid is less than the above range, the moisture retention effect of the skin as described above is not sufficient, and when the content of the hyaluronic acid exceeds the above range, there is a problem that a sticky feeling of use appears. The content of preferably satisfies the above range.

In addition, the hydrolyzed collagen is a mixture of small peptides generated through the hydrolysis of collagen, which reduces the flaking phenomenon that occurs when the skin becomes dry and keratin occurs, restores elasticity, and makes dry or damaged skin lively. have efficacy The content of the hydrolyzed collagen may be 0.001 wt% to 0.03 wt%, preferably 0.005 wt% to 0.015 wt%, more preferably 0.007 wt% to 0.012 wt%, based on the total weight of the cosmetic composition can be When the content of the hydrolyzed collagen is less than the above range, prevention of drying of the skin and recovery of damaged skin do not occur sufficiently, and when the content of the hydrolyzed collagen exceeds the above range, there is a problem that a sticky feeling appears, The content of the hydrolyzed collagen preferably satisfies the above range.

In addition, the cosmetic composition according to the present invention contains retinol in an amount of 0.01% to 0.5% by weight, preferably sodium pyruvate 0.03 to 3% by weight based on the total weight of the cosmetic composition, and also niacinamide 0.5 to 1.5% by weight, 0.007 to 0.015% by weight of hyaluronic acid, and 0.007 to 0.015% by weight of hydrolyzed collagen may be further included. The cosmetic composition has the above composition, and when the cosmetic composition is applied to the skin, it relieves skin irritation caused by retinol, and at the same time, the efficacy of retinol for wrinkles, acne, psoriasis, ichthyosis It shows an improvement effect such as.

In addition, the cosmetic composition according to the present invention may include, without limitation, commonly acceptable ingredients in addition to the above active ingredients, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. may include

On the other hand, the cosmetic composition according to the present invention is a solution, external ointment, nutrient lotion, massage cream, pack, lotion, foam, softening lotion, soft water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, One or more formulations selected from the group consisting of sun oil, suspension, emulsion, paste, gel, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray can be prepared. However, the present invention is not limited thereto.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but is not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, additional chloro propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan may be used. have.

When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. may be used as carrier components. can

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcositate, fatty acid amide as carrier components Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only provided by way of illustration to explain the present invention in more detail, and it is for those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples. it will be self-evident

Example

Test Example 1. Cytotoxicity test of pyruvate

In order to confirm whether the pyruvate according to the present invention is toxic to skin cells, the following experiment was conducted.

Specifically, in 37 human normal keratinocytes (Human epidermal neonatal keratinocyte cells, NHEK-Neo) it is Ron after its purchase from (Lonza, Inc. Walkersville, Maryland material) subculturing a CO ₂ incubator (CO ₂ incubator) C, 5% CO ₂ Incubated under conditions. The cell culture was according to Lonza's instructions. ^{KGM TM} Gold ^TM SingleQuots ^TM supplements (00192152, Lonza) (ingredient: BPE (Bovine pituitary extract)), human epidermal growth factor in 500 ml of KBM ^TM Gold ^TM Basal Medium (KBM-Glod, 00192151, Lonza) medium growth factor, hEGF), Insulin, Hydrocortisone, Transferrin, Epinephrine, and Gentamycin Suflate + Amphofericin-B (Gentamycin Suflate + Amphofericin-B: GA-1000)) A KGM-Gold Bullet Kit (KGM-Glod Bullet Kit, 00192060, Lonza) was used. After culturing human normal keratinocytes in the medium, the experiment was carried out in the 80% confluency state in the 80% confluency state.

The presence or absence of cytotoxicity of sodium pyruvate in the cultured epidermal cells was analyzed using a CCK-8 kit (Cell Counting Kit-8; Dojindo Molecular Technologies).

Specifically, the normal human keratinocytes were cultured in a 60 mm dish ^{under the condition of the number of cells in 2.5 × 10 5} wells, and after 24 hours, sodium pyruvate was added at concentrations of 0 mM (control), 1 mM and 3 mM, and again for 24 hours. incubated during Thereafter, the medium was replaced with 100 μL of CCK-8 solution and 900 μL of medium every 0, 24 h, and 48 h, and after reacting for 1 hour, absorbance was measured at 450 nm and 600 nm with a microplate reader after 24 hours and 48 hours. The difference was calculated to determine the cell viability.

The results are as shown in FIGS. 1 and 2 below.

As shown in FIG. 1 , as a result of treatment with pyruvate (1 mM and 3 mM) according to the present invention, the survival rate of normal human keratinocytes showed a tendency to increase over time, the same as that in the case where skin brate was not treated. could check From this, it can be seen that the pyruvate according to the present invention has no or weak toxicity to normal human keratinocytes. Furthermore, it was shown to be effective for cell proliferation as the number of cells was rather increased.

In addition, as shown in FIG. 2 , looking at the cell viability after 48 hours of treatment with the pyruvate according to the present invention, the viability of normal human keratinocytes when the concentration of pyruvate is 3 mM compared to the control group, It was found to be non-toxic or mildly toxic. Furthermore, it was shown to be effective for cell proliferation as the number of cells was rather increased.

From this, it was confirmed that the pyruvate according to the present invention did not exhibit cytotoxicity up to a concentration of 3 mM.

Test Example 2-1. Measurement of relative mRNA expression levels in keratinocytes (HAS2, CLDN1, FLG)

In order to confirm the drying improvement effect of retinol of pyruvate according to the present invention, the following experiment was conducted.

A control group in which no normal human keratinocytes were treated, a comparative example in which 1 μM retinol was treated, and an example in which normal human keratinocytes treated with 1 μM retinol were treated with sodium pyruvate at a concentration of 3 mM were compared.

First, as a control, normal human keratinocytes (5×10 ⁵ cells) untreated were inoculated into a 6-well plate, cultured for 48 hours, RNA extraction, cDNA synthesis, and Q-PCR. As a result, the relative mRNA expression levels of HAS2, CLDN1, and FLG of the normal human keratinocytes were measured.

In addition, in order to confirm skin irritation caused by retinol, as a comparative example, relative mRNA expression levels of HAS2, CLDN1, and FLG were measured in normal human keratinocytes treated with 1 μM retinol.

The results are as shown in FIG. 3 below.

Specifically, it was confirmed that the relative mRNA expression levels of CLDN1 and FLG decreased in normal human keratinocytes treated with 1 μM retinol. From this, it was confirmed that retinol exhibits inflammatory stimulation on skin cells.

In addition, in order to confirm the skin irritation alleviation effect of retinol by the pyruvate according to the present invention, as an example, after treating normal human keratinocytes treated with 1 μM retinol with sodium pyruvate at a concentration of 3 mM, The relative mRNA expression levels of HAS, CLDN1, and FLG were measured.

The results are as shown in FIG. 3 below.

Specifically, the relative mRNA expression levels of CLDN1 and FLG were decreased by retinol treatment, but it was confirmed that the relative mRNA expression levels of HAS2, CLDN1, and FLG increased when treated with sodium pyruvate.

From this, it was confirmed that the pyruvate according to the present invention is effective in alleviating skin irritation caused by retinol.

Test Example 2-2. Measurement of relative mRANA expression levels in keratinocytes (K1, K10, FLG, CXCL1, CXCL2, CCL20)

The following experiments were conducted to confirm the efficacy of the cosmetic composition comprising retinol and pyruvate according to the present invention as an active ingredient to improve skin wrinkles, prevent skin dryness, and inhibit skin inflammation.

Normal human keratinocytes treated with none (control), normal human keratinocytes treated with 0.5 mg/ml retinoic acid (RA), normal human keratinocytes treated with 0.5 mg/ml retinol (ROL), and these Human normal keratinocytes treated with sodium pyruvate at a concentration of 1 mM were compared.

First, untreated human normal keratinocytes (5×10 ⁵ cells) were inoculated into a 6-well plate, cultured for 48 hours, RNA extraction, cDNA synthesis, and Q-PCR were performed. , The relative mRNA expression levels of K1 (Keratin 1), K10 (Keratin 10), FLG (Filaggrin), CXCL1, CXCL2, and CCL20 of the normal human keratinocytes were measured. In addition, after treating the normal human keratinocytes with retinoic acid (RA), which is a product converted after retinol (ROL) or retinol is introduced into the cells, K1 (Keratin 1) and K10 (Keratin 10) of the normal human keratinocytes ), FLG (Filaggrin), CXCL1, CXCL2, and the relative mRNA expression levels of CCL20 were measured.

In addition, after treatment with 1 mM sodium pyruvate salt to human normal keratinocytes untreated, retinol (ROL)-treated human normal cells and retinoic acid (RA)-treated normal human keratinocytes, the human normal keratinocytes The relative mRNA expression levels of K1 (Keratin 1), K10 (Keratin 10), FLG (Filaggrin), CXCL1, CXCL2, and CCL20 were measured.

The results are as shown in FIG. 4 below.

Specifically, it was confirmed that the relative mRNA expression levels of K1 (Keratin 1) and K10 (Keratin 10) were decreased in normal human keratinocytes treated with 1 mM sodium pyruvate. From this, when treating normal human keratinocytes treated with retinol (ROL) or retinoic acid (RA) with sodium pyruvate, compared to the case of treating only retinol (ROL) or retinoic acid (RA), keratinocytes It was confirmed that by inhibiting differentiation and promoting proliferation, it exhibits the efficacy of improving skin wrinkles.

In addition, it was confirmed that the relative mRNA expression level of FLG (Filaggrin) significantly increased in normal human keratinocytes treated with 1 mM sodium pyruvate. The FLG (Filaggrin) is used as an indicator of the water content. From this, when the sodium pyruvate is treated with retinol (ROL) or retinoic acid (RA)-treated human normal keratinocytes, simply retinol (ROL) or It was confirmed that the moisture content was significantly increased compared to the case of treating only retinoic acid (RA). That is, by adding pyruvate to the cosmetic composition containing retinol of the present invention, it was confirmed that the effect of preventing skin dryness caused by retinol was exhibited.

In addition, it was confirmed that the relative mRNA expression levels of CXCL1, CXCL2, and CCL20 were significantly reduced in normal human keratinocytes treated with 1 mM sodium pyruvate. From this, when treating normal human keratinocytes treated with retinol (ROL) or retinoic acid (RA) with sodium pyruvate, compared to the case of treating only retinol (ROL) or retinoic acid (RA), skin inflammation occurs was found to significantly inhibit the That is, it was confirmed that by adding pyruvate to the cosmetic composition containing retinol of the present invention, the effect of significantly inhibiting the occurrence of skin inflammation caused by retinol was confirmed.

The cosmetic composition comprising retinol and pyruvate as active ingredients according to the present invention can be commercialized in various formulations, and an appropriate content ratio can be controlled in consideration of the functionality, cost, and other conditions of the product to be implemented. Hereinafter, ointments, nutrient lotions, massage creams, packs and lotions are exemplified as formulation examples of the present invention, but the formulation of the cosmetic composition according to the present invention is not limited thereto.

[Formulation Example 1] Ointment

An ointment containing retinol and pyruvate according to the present invention was prepared by mixing the ingredients in Table 1 including the oil phase component and the aqueous phase component.

Ingredients content (wt%) Purified water remaining amount active ingredient retinol 0.5 Pyruvate Sodium Salt One Niacinamide 0.9 hyaluronic acid 0.01 hydrolyzed collagen 0.01 Sum 0.97 Caprin/Caprylic Triglyceride 10.00 liquid paraffin 10.00 Sorbitan Sesquioleate 6.00 Octyldodeces-25 9.00 Cetylethylhexanoate 10.00 squalane 1.00 salicylic acid 1.00 glycerin 15.00 sorbitol 10.00

[Formulation Example 2] Nutrient lotion

A nutritional lotion containing retinol and pyruvate according to the present invention was prepared by mixing the ingredients in Table 2 including the oil phase component and the water phase component.

Ingredients content (wt%) Purified water remaining amount active ingredient retinol 0.5 Pyruvate Sodium Salt One Niacinamide 0.9 hyaluronic acid 0.01 hydrolyzed collagen 0.01 Sum 0.97 beeswax 4.00 Polysorbate 60 1.50 Sorbitan Sesquioleate 1.50 liquid paraffin 0.50 Montana 202 (Manufacturer: Sppic) 5.00 glycerin 3.00 butylene glycol 3.00 propylene glycol 3.00 Carboxyvinyl Polymer 0.10 triethanolamine 0.20 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 3] Massage Cream

A massage cream containing retinol and pyruvate according to the present invention was prepared by mixing the ingredients in Table 3 including the oil phase component and the water phase component.

Ingredients content (wt%) Purified water remaining amount active ingredient retinol 0.5 Pyruvate Sodium Salt One Niacinamide 0.9 hyaluronic acid 0.01 hydrolyzed collagen 0.01 Sum 0.97 beeswax 10.00 Polysorbate 60 1.50 sebum 60 hydrogenated castor oil 2.00 Sorbitan Sesquioleate 0.80 liquid paraffin 40.00 squalane 5.00 Montana 202 (Manufacturer: Sppic) 4.00 glycerin 5.00 butylene glycol 3.00 propylene glycol 3.00 triethanolamine 0.20 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 4] Pack

A pack containing retinol and pyruvate according to the present invention was prepared by mixing the ingredients in Table 4 including the oil phase component and the aqueous phase component.

Ingredients content (wt%) Purified water remaining amount active ingredient retinol 0.5 Pyruvate Sodium Salt One Niacinamide 0.9 hyaluronic acid 0.01 hydrolyzed collagen 0.01 Sum 0.97 polyvinyl alcohol 13.00 Sodium Carboxymethyl Cellulose 0.20 glycerin 5.00 allantoin 0.10 ethanol 6.00 PEG-12 Nonylphenyl Ether 0.30 Polysorbate 60 0.30 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 5] Lotion

A lotion containing retinol and pyruvate according to the present invention was prepared by mixing the ingredients in Table 5 including the oil phase component and the water phase component.

Ingredients content (wt%) Purified water remaining amount active ingredient retinol 0.5 Pyruvate Sodium Salt One Niacinamide 0.9 hyaluronic acid 0.01 hydrolyzed collagen 0.01 Sum 0.97 cetostearyl alcohol 2.00 EDTA 2Na 0.20 mineral oil 5.00 Preservatives, Colors, Flavors appropriate amount

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, these specific descriptions are only preferred embodiments, and it is clear that the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (14)

A cosmetic composition for alleviating skin irritation caused by retinol, comprising retinol and pyruvate as active ingredients. According to claim 1,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the content of the retinol is 0.01% to 0.5% by weight. According to claim 1,
A cosmetic for alleviating skin irritation caused by retinol, wherein the pyruvate is selected from the group consisting of lithium pyruvate, sodium pyruvate, potassium pyruvate, magnesium pyruvate, calcium pyruvate, zinc pyruvate, manganese pyruvate, and mixtures thereof composition. According to claim 1,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the content of the pyruvate is 0.01% to 5% by weight based on the total weight of the cosmetic composition. According to claim 1,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the weight ratio (molar ratio) of pyruvate to retinol is 1:10 to 1:100. According to claim 1,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the cosmetic composition further comprises niacinamide, hyaluronic acid and hydrolyzed collagen. 7. The method of claim 6,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the content of the niacinamide is 0.1% to 2% by weight based on the total weight of the cosmetic composition. 7. The method of claim 6,
The cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the content of the hyaluronic acid is 0.001% to 0.03% by weight based on the total weight of the cosmetic composition. 7. The method of claim 6,
A cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the content of the hydrolyzed collagen is 0.001% to 0.03% by weight based on the total weight of the cosmetic composition. 3. The method of claim 2,
The cosmetic composition comprises 0.03 to 3% by weight of sodium pyruvate, 0.5 to 1.5% by weight of niacinamide, 0.007 to 0.015% by weight of hyaluronic acid, and 0.007 to 0.015% by weight of hydrolyzed collagen, based on the total weight. A cosmetic composition for alleviating skin irritation caused by According to claim 1,
The cosmetic composition for alleviating skin irritation caused by retinol, characterized in that the cosmetic composition has any one formulation selected from among ointments, nourishing lotions, massage creams, packs and lotions. According to claim 1,
The cosmetic composition is a cosmetic composition for alleviating skin irritation caused by retinol, characterized in that it improves skin wrinkles. According to claim 1,
The cosmetic composition is a cosmetic composition for alleviating skin irritation caused by retinol, characterized in that it prevents skin dryness. According to claim 1,
The cosmetic composition is a cosmetic composition for alleviating skin irritation caused by retinol, characterized in that it suppresses skin inflammation.

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