KR102623565B1 - Composition for improving skin comprising β-pinene as active ingredient - Google Patents

Abstract

The present invention relates to a composition for improving skin, and to a composition containing beta-pinene as an active ingredient and having excellent skin whitening, wrinkle improvement, elasticity promotion, and skin moisturizing effects. The composition according to the present invention is safe for the skin and significantly exhibits skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing effects in a small effective amount, and can be usefully used in medicine, cosmetics, or external skin compositions.

Description

Composition for improving skin comprising beta-pinene as active ingredient}

The present invention relates to a composition for skin improvement that exhibits skin whitening, skin wrinkle improvement, elasticity enhancement, and skin moisturizing effects.

Skin color or brightness is genetically determined by the concentration and distribution of melanin in a person's skin, but is also affected by environmental or physiological conditions such as solar ultraviolet rays, fatigue, or stress. Melanin is produced by sequentially converting tyrosine, a type of amino acid, into DOPA and dopaquinone through the enzyme tyrosinase as a catalyst, followed by a non-enzymatic oxidation reaction. Although the path by which melanin is produced is known, the cause of tyrosinase triggering in the mechanism that induces melanin synthesis has not yet been revealed in detail.

Meanwhile, commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C (L-Ascorbic acid), or their derivatives. There are various plant extracts, etc. By inhibiting the synthesis of melanin pigment, they not only brighten skin tone and have a skin whitening effect, but can also improve skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or genetics. However, when applied to the skin, there is a limit to the amount used due to skin safety issues such as irritation or redness, or the effect is so small that no practical effect can be expected.

Collagen is a major matrix protein produced by fibroblasts in the skin and exists in the extracellular matrix. Its important functions include mechanical rigidity of the skin, resistance and adhesion of connective tissue, support for cell adhesion, and cell adhesion. Induction of division and differentiation (during organism growth or wound healing) is known. Collagen decreases due to age and photoaging caused by ultraviolet irradiation, and collagen reduction is promoted by collagenase enzyme activity that decomposes collagen. This is known to be closely related to the formation of wrinkles on the skin.

Currently, retinoids, adenosine, protein derived from animal placenta, or chlorella extract are known as wrinkle-improving cosmetics. The most well-known retinol is a substance that promotes collagen synthesis and inhibits the elastase enzyme, but it is unstable and its usage is limited due to safety issues such as irritation or redness when applied to the skin. Chlorella extract, etc. It is known that the effect is so small that it is difficult to actually expect an improvement in skin wrinkles.

Hyaluronic acid is a type of glycosaminoglycans and is a chain-shaped polymer polysaccharide in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked. It has the property of forming a gel by combining with a large amount of water, so it has high viscosity and elasticity. Hyaluronic acid is a major component of the extracellular matrix and has been reported to be involved in water retention, maintenance of intercellular space, storage and diffusion of cell growth factors and nutrients, as well as cell division, differentiation, and migration. there is.

It has been reported that more than 50% of the hyaluronic acid present in the mammalian body is distributed in the skin, especially in the intercellular spaces of the epidermis and the connective tissue of the dermis. It is known that such hyaluronic acid is mainly synthesized by keratinocytes and fibroblasts. . The amount of hyaluronic acid in human skin has been reported to decrease with aging, and the decrease in hyaluronic acid in the skin is believed to be one of the direct causes of decreased skin elasticity and decreased moisture content due to aging (Non-Patent Documents 1 to 3). In addition, hyaluronic acid is known to be involved in maintaining the structure of the stratum corneum and skin barrier function (Non-patent Document 4). Therefore, hyaluronic acid is directly related to skin moisturization.

However, hyaluronic acid, which has the above effects, has a large molecular weight and is not easily absorbed into the skin. In addition, a method of injecting hyaluronic acid into the skin is currently being used, but a method of increasing hyaluronic acid synthesis within skin cells is more effective. Therefore, research is being actively conducted on methods to increase the production of hyaluronic acid in the human body, but no notable research results are yet known.

Therefore, there is an urgent need to develop ingredients that are safe for the living body, have stable active ingredients, and, above all, are more effective than existing substances that have skin whitening, wrinkle improvement, elasticity promotion, or moisturizing effects.

Biochem Biophys Acta 279, 265-275 Carbohydr Res 159, 127-136 Int J Dermatol 33, 119-122 J Cosmet Dermatol. 2007 Jun, 6(2), 75-82

Accordingly, the present inventors found that beta-pinene improves wrinkles and improves elasticity by promoting collagen synthesis in skin fibroblasts, exhibits a whitening effect by inhibiting melanin synthesis, and increases hyaluronic acid production in keratinocytes to moisturize the skin. The present invention was completed by confirming that .

Therefore, the purpose of the present invention is to provide a new use for beta-pinene that is safe for the human body, has fewer side effects, and has excellent skin whitening, wrinkle improvement, elasticity promotion, and moisturizing effects.

Another object of the present invention is to provide a medicine, cosmetic, or external skin preparation containing the beta-pinene as an active ingredient.

As a means to solve the above problems, the present invention provides a composition for skin whitening, wrinkle improvement, elasticity promotion and skin moisturizing containing beta-pinene as an active ingredient for the production of medicines, cosmetics or external skin preparations.

Beta-pinene according to the present invention improves wrinkles and improves elasticity by promoting collagen synthesis in skin fibroblasts, exhibits a whitening effect by inhibiting melanin synthesis, and increases hyaluronic acid production in keratinocytes to provide a skin moisturizing effect. As a result, it can be used in the production of medicines, cosmetics, or external skin preparations.

Hereinafter, the configuration of the present invention will be described in detail.

In order for skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing ingredients to have excellent effects when applied to actual skin, they exhibit highly active skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing activities at low concentrations, and are absorbed through the skin. Must have excellent ability. In addition, it has low volatility so that it can remain for a sufficient period of time to exhibit skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing effects, and the active ingredients remain stable in the composition or on the skin, and can be manufactured as medicine, cosmetics, or external skin preparations. It is desirable that it is easy and safe for the skin. However, among known components, components that satisfy all of the above characteristics are rare. For example, some skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing ingredients have excellent skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing properties even at low concentrations in in vitro experiments, but their ability to penetrate and absorb through the skin is low, so they are not effective in actual skin moisturizing. It is difficult to apply to the skin. Other active ingredients have low hydrophilicity, making them difficult to formulate as medicine, cosmetics, or external skin products. In addition, some skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing ingredients may lose their effectiveness before application to the skin because the active ingredients are decomposed or transformed into other compounds when exposed to heat, light, or oxygen.

As can be seen in the examples below, beta-pinene exhibits significantly excellent skin whitening, wrinkle improvement, elasticity promotion, and skin moisturizing effects at low concentrations, so it can be used in medicines, cosmetics, It can be used as an active ingredient in external skin compositions.

Therefore, the present invention provides a composition for skin whitening, wrinkle improvement, elasticity promotion, and skin moisturization containing beta-pinene as an active ingredient.

Beta-pinene used as an active ingredient in the composition of the present invention may be a compound represented by the following formula (1).

[Formula 1]

The compound represented by Formula 1 may be named beta-pinene, beta-pinene, or 6,6-Dimethyl-2-methylenebicyclo[3.1.1]heptane. Additionally, the molecular formula of the compound is C ₁₀ H ₁₆ and the molecular weight is 136.23.

In the present invention, the method of obtaining beta-pinene is not particularly limited, and it can be chemically synthesized according to a method known in the art or a commercially available material can be used.

In the present invention, the beta-pinene may exist in an unsolvated form as well as a solvated form including hydrate or ethanolate. The beta-pinene may exist in crystalline or amorphous form, and all such physical forms are included within the scope of the present invention.

In the present invention, the beta-pinene may be included in an amount of 0.00001 to 10% by weight, for example, 0.0001 to 10% by weight, based on the total weight of the composition. If the composition of the present invention contains less than 0.00001% by weight of beta-pinene, sufficient skin whitening, wrinkle improvement, elasticity enhancement and skin moisturizing effects cannot be expected, and if it contains more than 10% by weight, it may cause unwanted side effects such as allergies. Reactions may occur or skin safety issues may arise.

The compositions of the present invention can be used for preparing pharmaceutical formulations.

The composition may further contain one or more active ingredients that exhibit the same or similar functions. For example, it may include known skin whitening, wrinkle improvement, elasticity enhancement, and skin moisturizing ingredients. If additional skin whitening, wrinkle improvement, elasticity improvement, and skin moisturizing ingredients are included, the skin whitening, wrinkle improvement, elasticity improvement, and skin moisturizing effects of the composition of the present invention can be further improved. When adding the above ingredients, skin safety due to combined use, ease of formulation, and stability of the active ingredients can be taken into consideration. In one embodiment of the present invention, the composition is a skin whitening ingredient known in the art, a substance that inhibits tyrosinase enzyme activity such as kojic acid, arbutin, etc., and hydroquinone. , Vitamin-C (L-Ascorbic acid); Wrinkle improvement and elasticity enhancement ingredients known in the art include retinoic acid, TGF, protein derived from animal placenta, betulinic acid, and chlorella extract; and one or two or more ingredients selected from the group consisting of their derivatives and various plant extracts. Additional ingredients may be included in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and ease of formulation of the beta-pinene.

Additionally, the composition of the present invention may further include a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain various ingredients such as buffers, sterile water for injection, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates.

The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various oral and parenteral formulations during clinical administration. When formulated, commonly used fillers and extenders are used. It can be prepared using diluents or excipients such as binders, wetting agents, disintegrants, and surfactants.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, such as starch, calcium carbonate, and water, in the pharmaceutical composition of the present invention. It can be prepared by mixing sucrose, lactose, gelatin, etc.

In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included.

Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.

In addition, the composition of the present invention can preferably be used as a parenteral preparation, and the parenteral preparation may include, for example, a pharmaceutically acceptable base such as vaseline or stearyl alcohol; Pharmaceutically acceptable surfactants such as polysorbate or sorbitan sesquioleate; Pharmaceutically acceptable moisturizers such as glycerin; pharmaceutically acceptable solvent; It can be manufactured by a conventional parenteral preparation method of homogeneously mixing flavoring agents, colorants, stabilizers, or viscosifiers.

In the present invention, 'whitening effect' refers to not only brightening skin tone by inhibiting the synthesis of melanin pigment, but also improving skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or genetics.

In the present invention, the 'wrinkle improvement effect' refers to suppressing or inhibiting the formation of skin wrinkles caused by genes, reduction of collagen present in the skin dermis, or external environment, or alleviating wrinkles that have already been formed. It says what to do.

In the present invention, the 'elasticity enhancing effect' refers to increasing the elasticity of the skin by suppressing the decrease in hyaluronic acid, which connects elastin and collagen present in the dermal layer.

In the present invention, 'moisturizing or skin moisturizing effect' refers to maintaining moisture homeostasis of the skin by appropriately controlling moisture loss (water evaporation) of the skin, thereby increasing moisture and maintaining a moist state. When hyaluronic acid or collagen in the skin decreases, moisture can be lost, and when hyaluronic acid or collagen increases, moisture can increase. Additionally, in the present invention, skin moisturizing can help improve skin wrinkles or improve skin elasticity.

In the present invention, 'effective amount' refers to the amount of beta-pinene that can exhibit a whitening effect, improve wrinkles, improve elasticity, or exhibit a moisturizing effect. When the composition of the present invention contains an effective amount of beta-pinene, it can provide desirable skin whitening effects, wrinkle improvement effects, elasticity enhancement effects, and moisturizing effects. The effective amount of beta-pinene included in the composition of the present invention will vary depending on the form in which the composition is manufactured, the method in which the compound is applied to the skin, and the time it remains on the skin. For example, when the composition is commercialized as a pharmaceutical, it may contain beta-pinene at a higher concentration than when it is commercialized as a cosmetic that is routinely applied to the skin. Therefore, the daily dosage may be 0.1 to 100 mg/kg, preferably 30 to 80 mg/kg, and more preferably 50 to 60 mg/kg, based on the amount of beta-pinene, which is the active ingredient. It may be administered 1 to 6 times a day.

The composition of the present invention can be used alone or in combination with surgery, radiation therapy, hormone therapy, chemical therapy, and methods using biological response modifiers.

The pharmaceutical formulation of the present invention may include a skin topical formulation.

When the beta-pinene is used as an external skin agent, it is additionally added with fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, and water. , ionic or non-ionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, or commonly used in external preparations for the skin. It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients. Additionally, the ingredients may be introduced in amounts commonly used in the field of dermatology.

When the beta-pinene is provided in an external skin formulation, it is not limited thereto, but may have a formulation such as ointment, patch, gel, cream, or spray.

Additionally, the composition for skin whitening, wrinkle improvement, elasticity promotion, and skin moisturizing of the present invention can be used to prepare cosmetic formulations.

The cosmetic formulation may be in the form of a general emulsified formulation or solubilized formulation. For example, lotion such as flexible lotion or nourishing lotion, emulsion such as facial lotion, body lotion, cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, liquid such as nutritional cream, moisture cream, eye cream, etc. It may have a formulation such as foundation type, solid type or spray type, powder, makeup remover such as cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap, body wash, etc.

In addition to beta-pinene, the cosmetics contain fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, and ionic agents. or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other commonly used in cosmetics. It may contain auxiliaries commonly used in the field of cosmetics, such as ingredients.

The auxiliaries may be contained in various ways depending on the formulation.

When the cosmetic formulation is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or mixtures thereof, etc. This can be used as a supplement.

When the cosmetic formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as auxiliaries. In particular, when the cosmetic formulation is a spray, chlorofluorocarbons may be added. May contain propellants such as lohydrocarbon, propane/butane or dimethyl ether.

When the cosmetic formulation is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as an auxiliary agent, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan fatty acid ester are used. It can be.

When the cosmetic formulation is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, Aluminum metahydroxide, bentonite, agar, or tracant may be used as auxiliaries.

When the cosmetic formulation is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, or sugars may be used.

The cosmetic formulation may contain a relatively high concentration of beta-pinene in the case of wash-off type cosmetics such as makeup removers and detergents in which the active ingredient stays on the skin for a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotions, emulsions, creams, and essences, where the active ingredients stay on the skin for a long period of time, even if they contain a lower concentration of beta-pinene than wash-off type cosmetics, It will be fine. Although not limited thereto, in one embodiment of the present invention, the composition may include beta-pinene in an amount of 0.0001% by weight to 10% by weight, for example, 0.0001% by weight to 1% by weight, based on the total weight of the composition. If the composition of the present invention contains less than 0.0001% by weight of beta-pinene, sufficient skin whitening, wrinkle improvement, elasticity enhancement and skin moisturizing effects cannot be expected, and if it contains more than 10% by weight, it may cause unwanted side effects such as allergies. This is to prevent reactions that may occur or problems with skin safety.

Hereinafter, the present invention will be explained in detail by the following examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

Reference example One: beta-pinene (β- pinene ) substance information

[Formula 1]

Substance name: β-pinene

CAS No.: 18172-67-3

Molecular formula: C ₁₀ H ₁₆

Molecular Weight: 136.23

Example 1: Effect of increasing total collagen amount

After beta-pinene was added to the culture medium of human-derived fibroblasts, the total amount of collagen synthesized at the cellular level was measured. The total amount of synthesized collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

Before the experiment, cytotoxicity was evaluated on human-derived fibroblasts at concentrations of 0.001 ppm, 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm (method of performing MTT test by culturing fibroblasts) [Reference: Mossman T (1983). Rapid Colorimetric Assay for Cellular Growth & Survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55-63], and a non-cytotoxic concentration was selected to measure the total amount of collagen.

In the experiment, the final concentrations of beta-pinene were 1 ppm and 10 ppm, respectively. Each sample was added to the culture medium of human fibroblasts and cultured for 1 day, and then the culture medium was taken and the total amount of collagen at each concentration was measured using the PICP EIA kit. Measured at 450 nm using a spectrophotometer. To compare the effects, the total amount of collagen was measured in the same way for the fibroblast culture medium without any addition (control group) and the sample to which TGFb was added to a final concentration of 10 ppm. The total amount of collagen was measured by UV absorbance, and the rate of increase in the total amount of collagen was calculated as the ratio of the total amount of collagen relative to the control group, and the results are summarized in Table 1 below.

sample Absorbance average Collagen increase rate (%) Beta-pinene (1 ppm) 510 113 Beta-pinene (10 ppm) 577 128 Control group (no additives) 451 - TGFb (10 ppm) 586 130

(number of repetitions=6)

As can be seen from the results in Table 1, beta-pinene (10 ppm) is a natural substance and has an excellent collagen synthesis effect on par with TGFb (10 ppm), which is known to have the ability to synthesize collagen. Therefore, it was confirmed that beta-pinene can be used as an effective active ingredient in cosmetics, pharmaceuticals, and external skin preparations for improving wrinkles and improving elasticity.

Example 2: Effect of increasing hyaluronic acid concentration in human-derived keratinocytes

Human-derived keratinocytes were cultured at 1x10 ⁵ cells/ml in DMEM medium containing 10% fetal bovin serum, distributed at 500 ㎕ each in a 24-well plate, and cultured for 18 hours. Then, washed twice with DMEM medium without bovine serum. Beta-pinene was added at different concentrations (1 ppm, 10 ppm). As a negative control, the same amount of ethanol was treated, and as a positive control, 100 ppm of glucosamine hydrochloride (Sigma-aldrich), which is known to promote hyaluronic acid production, was treated. After culturing for 24 hours, the cell culture medium was recovered and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), and the results are shown in Table 2 below.

sample Hyaluronic acid relative concentration (%) Beta-pinene (1 ppm) 118 Beta-pinene (10 ppm) 133 Glucosamine (100ppm) 136 Control group (no additives) 100

(number of repetitions=4)

The relative concentration of hyaluronic acid shown in Table 2 was quantified based on the hyaluronic acid concentration of the negative control group (100%) in the experimental group treated with beta-pinene.

Looking at the results shown in Table 2 above, it was confirmed that beta-pinene increases hyaluronic acid production in human-derived keratinocytes in a concentration-dependent manner. In particular, beta-pinene showed a similar relative concentration of hyaluronic acid even in an amount 10 times smaller than that of the positive control, showing that the hyaluronic acid promoting effect was significantly excellent.

Therefore, it was confirmed that beta-pinene can be used as an effective active ingredient in cosmetics, pharmaceuticals, and external skin preparations for skin moisturization, and that it can also help improve skin wrinkles and improve elasticity through the skin moisturizing effect. there is.

Example 3: Whitening effect - effect of reducing total melanin amount

Beta-pinene was added to the culture medium of B-16 mouse melanoma cells and the whitening effect was confirmed by measuring the total amount of melanin at the cellular level (Lotan R., Lotan D. Cancer Res. 40:3345- 3350, 1980). Before the experiment, toxicity was evaluated for rat melanoma cells, a non-toxic concentration was selected, and whitening evaluation was performed.

Beta-pinene was added to the culture medium to a final concentration of 1 ppm and 10 ppm, and arbutin as a control was added to the medium to a final concentration of 100 ppm, and then treated with B-16 melanoma cells and cultured for 3 days.

Afterwards, the cultured cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. To the removed cells, 1 ml of sodium hydroxide solution (1N concentration) was added and boiled for 10 minutes to dissolve the melanin, and the amount of melanin produced was measured by measuring the absorbance at 400 nm using a spectrophotometer.

The amount of melanin was measured by expressing the absorbance per unit cell (1×10 ⁶ cell). The total amount of melanin relative to the control group was calculated as the inhibition rate (%), and the results are summarized in Table 3 below.

sample Melanin production amount (abs) Inhibition rate (%) Control (DMSO, 10ppm) 0.31 - Positive control group (arbutin, 100ppm) 0.200 35.6 Beta-pinene (10ppm) 0.172 44.5 Beta-pinene (1ppm) 0.250 19.1

(number of repetitions=4)

As can be seen from the results in Table 3, compared to arbutin, a known whitening substance, beta-pinene produces a lower total amount of melanin even when applied at a much lower concentration to cultured rat melanoma cells, thereby promoting melanin production. It was confirmed that the inhibitory effect was remarkably excellent.

Therefore, it was confirmed that beta-pinene can be used as an effective active ingredient in cosmetics, pharmaceuticals, and external skin preparations for skin whitening.

Claims (7)

A composition for skin whitening containing beta-pinene as an active ingredient. According to claim 1,
The composition is a skin whitening composition for manufacturing skin whitening medicines, cosmetics, or external skin preparations. According to claim 1,
A composition for skin whitening wherein beta-pinene is contained in an amount of 0.00001 to 10% by weight based on the total weight of the composition. delete delete delete delete