KR20210062986A - Artificial feed composition of Protaetia brevitarsis - Google Patents

Abstract

The present invention relates to an artificial feed composition for breeding Protaetia brevitarsis, and more particularly, to an artificial feed composition capable of significantly increasing the number of spawning, a spawning period, and a survival period of the Protaetia brevitarsis. The artificial feed composition for breeding the Protaetia brevitarsis according to the present invention significantly increases the number of spawning, and the spawning period and the survival period of the Protaetia brevitarsis. The artificial feed composition according to the present invention can mass-breed the Protaetia brevitarsis throughout the year, thereby being utilized in various fields such as insect breeding, and furthermore in the pharmaceutical and food fields.

Description

Artificial feed composition for breeding white-spotted flower radish {Artificial feed composition of Protaetia brevitarsis}

The present invention relates to an artificial feed composition for breeding white-spotted radish, and more particularly, to an artificial feed composition capable of significantly increasing the number of eggs, spawning period and survival period of white-spotted radish.

White-spotted flower radish ( Protaetia brevitarsis ) is distributed in Korea, Japan, Taiwan, China, and Europe. Although there is a difference depending on the region, the white-spotted flower radish occurs twice a year from May to October and overwinters as 3rd instar mature larvae. In addition, the adult larvae are active during the day and are clustered, and the larvae live in humus soil and do not harm live plants. At 25°C, one generation survives for 120 to 150 days, and the period of each developmental stage is 10 days for eggs, 55 days for larvae, 30 days for pupa and 45 days for adults, and the average number of eggs is 68.

In oriental medicine, slugs, the larvae of scarabs, are called ‘manufacture’ and have been used for liver disease, thrush, tetanus, and diuretic effects. Recently, it is known that an antibiotic active peptide isolated from white spot flower radish has strong antibiotic activity against gram-positive and gram-negative bacteria. In addition, it has been found that it has antioxidant activity and the demand is increasing significantly.

Accordingly, the present inventors have completed the present invention by developing an artificial feed composition for increasing the breeding efficiency of white-spotted flower radish, and confirming the effect of increasing the number of white-spotted flower radish spawning, spawning period and survival period of the composition.

Accordingly, an object of the present invention is to provide an artificial feed composition for breeding white spot flower radish, comprising 3 to 15 parts by weight of casein, based on 100 parts by weight of the composition.

Another object of the present invention is to prepare a mixture by adding 0.5 to 3 parts by weight of agar and 3 to 15 parts by weight of casein with respect to 100 parts by weight of the mixture to provide a method for producing artificial feed for breeding white spot flower radish, including a will do

Another object of the present invention is to provide an artificial breeding method of white-spotted flower radish, comprising the step of feeding the artificial feed composition for white-spotted flower radish breeding.

In order to achieve the above object, the present invention provides an artificial feed composition for breeding white spot flower radish, comprising 3 to 15 parts by weight of casein, based on 100 parts by weight of the composition.

In addition, the present invention provides a method for producing artificial feed for breeding white-spotted radish, comprising the step of preparing a mixture by adding 0.5 to 3 parts by weight of agar and 3 to 15 parts by weight of casein with respect to 100 parts by weight of the mixture.

In addition, the present invention provides an artificial breeding method of white-spotted flower radish, comprising the step of feeding the artificial feed composition for white-spotted flower radish breeding.

It was confirmed that the artificial feed composition for breeding white-spotted radish according to the present invention remarkably increased the number of eggs, spawning period and survival period of white-spotted radish. This means that the artificial feed composition according to the present invention can be mass-bred throughout the year, and it can be used in various fields in insect breeding fields, as well as in pharmaceuticals and food fields.

1 is a diagram showing the results of confirming the number of spawning of white-spotted beetles according to the content of casein contained in an artificial feed composition.
2 and 3 are diagrams showing the results of confirming the spawning period of the white spot beetle adult according to the casein content contained in the artificial feed composition.
4 is a view showing the results of confirming the survival period of the white spot larvae according to the casein content contained in the artificial feed composition.

Hereinafter, the present invention will be described in detail.

According to an aspect of the present invention, the present invention provides an artificial feed composition for breeding white spot flower radish, comprising 3 to 15 parts by weight of casein, based on 100 parts by weight of the composition.

In the present invention, “casein” is a protein contained in milk and is an additive having many nutritional values, emulsifying power and water holding power. In addition, the casein, as a protein, not only contains the amino acids evenly for each type, but also improves the adhesion, viscosity, and emulsion stability of food. Accordingly, casein is used as an emulsifier, thickener, and stabilizer as an additive to improve the physical properties and feel of food.

In a preferred embodiment of the present invention, the composition contains casein in an amount of 5 to 10 parts by weight, more preferably 5 to 7 parts by weight, based on 100 parts by weight of the composition. When the content of the casein exceeds 15 parts by weight based on 100 parts by weight of the composition, there is a fear that the number of eggs, the duration of spawning and the survival period of the white-spotted radish are significantly reduced.

In one embodiment of the present invention, the artificial feed composition comprises sucrose, maltose, lactose, lactulose, trehalose, cellobiose and chitobiose ( chitobiose), it is preferable to further include at least one sugar member selected from the group consisting of chitobiose), and more preferably include sucrose.

The sucrose is a disaccharide in which one molecule of fructose and one molecule of glucose are linked by a glycosidic bond, and is a major carbon source in artificial feed. Sucrose contained in artificial feed is used for synthesizing glycogen present in all cells of insects, especially fat bodies, flying muscles, and epidermal cells of the digestive tract and body wall.

In a preferred embodiment of the present invention, the composition further comprises sucrose in an amount of 3 to 15 parts by weight, more preferably in an amount of 10 to 15 parts by weight, and more preferably in an amount of 13 to 15 parts by weight, based on 100 parts by weight of the composition. It is more preferable to include, but is not limited thereto. When the content of the sucrose is less than 3 parts by weight, there is a fear that a deficiency of nutrients required for the growth of white spot flower radish may occur.

In one embodiment of the present invention, the weight ratio of casein and sugar source is preferably 7:3 to 3:7, more preferably 7:3, and still more preferably, the weight ratio of casein and sucrose is 7:3. However, the present invention is not limited thereto.

In one embodiment of the present invention, the artificial feed composition further comprises at least one selected from the group consisting of sorbic acid, methyl-p-hydroxybenzoate and agar. can do.

The sorbic acid is an additive used to increase the preservation of processed foods by inhibiting the growth of microorganisms.

The methyl-p-hydroxybenzoic acid is a sterilization preservative, and prevents contamination and deterioration by microorganisms when artificial feed is exposed to the air or foreign substances are mixed.

The agar is one of the gelling agents (coagulant), in which dried agar agar is put in water, boiled and coagulated, and then freeze-dried and freeze-dried. The coagulation power of agar is 10 times that of gelatin, and the higher the concentration of agar, the easier it is to coagulate. Agarose contains 80% of indigestible polysaccharides called agarose and agaropectin, and also contains various minerals and vitamins.

In a preferred embodiment of the present invention, the composition preferably contains 0.5 to 3 parts by weight of agar, 0.05 to 1.5 parts by weight of sorbic acid, and 0.05 to 1.5 parts by weight of methyl-p-hydroxybenzoic acid, based on 100 parts by weight of the composition, It is not limited thereto.

In another embodiment of the present invention, the artificial feed composition may further include a vitamin complex or complex inorganic salts, but is not limited thereto. In addition, the artificial feed composition of the present invention may further include other nutrients within the range that those skilled in the art can expect.

The artificial feed composition according to the present invention was optimized for artificial breeding of white-spotted daisy, and it was confirmed that the number of eggs, spawning period, and survival period of the white-spotted daisy were significantly increased. Therefore, the artificial feed composition according to the present invention can increase the efficiency of breeding by increasing the number of spawning of white-spotted radish.

According to another aspect of the present invention, the present invention comprises the steps of preparing a mixture by adding 0.5 to 3 parts by weight of agar and 3 to 15 parts by weight of casein with respect to 100 parts by weight of the mixture; A manufacturing method is provided.

In one embodiment of the present invention, it may further include a step of heating until reaching 70 to 90 ℃ after mixing the agar and distilled water prior to the step, but is not limited thereto.

In one embodiment of the present invention, in the above step, one or more sugar sources selected from the group consisting of cross, maltose, lactose, lactulose, trehalose, cellobiose and chitobiose may be further added to the mixture, but is not limited thereto. does not Preferably, based on 100 parts by weight of the composition, 3 to 15 parts by weight of the sugar source, more preferably 13 to 15 parts by weight, may be included, but is not limited thereto. When the content of the sugar source is less than 3 parts by weight, there is a fear that a deficiency of nutrients required for the growth of white spot flower radish may occur.

In a preferred embodiment of the present invention, in the above step, the weight ratio of casein and sugar source is preferably 7:3 to 3:7, more preferably 7:3, and still more preferably, the weight ratio of casein and sucrose is 7 :3, but not limited thereto.

In one embodiment of the present invention, the step may be to further add one or more selected from the group consisting of sorbic acid or methyl-p-hydroxybenzoic acid to the mixture. Preferably, based on 100 parts by weight of the mixture, 0.05 to 1.5 parts by weight of sorbic acid and 0.05 to 1.5 parts by weight of methyl-p-hydroxybenzoic acid may be further added to the mixture, but is not limited thereto.

In another embodiment of the present invention, the step may further add a vitamin complex or complex inorganic salts to the mixture, but is not limited thereto. In addition, other nutrients within the range expected by those skilled in the art may be added to the mixture.

According to another aspect of the present invention, the present invention provides an artificial breeding method of white-spotted flower radish, comprising the step of feeding the artificial feed composition for rearing white-spotted flower radish.

The artificial breeding method according to the present invention has the advantage of being able to significantly increase the number of eggs laid, the duration of spawning, and the survival period of white-spotted radish, and can be mass-bred throughout the year.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples.

Example 1. Preparation of jelly-type artificial feed for breeding white-spotted flower radish

For the breeding of white spot flower radish, a jelly-type artificial feed with different ratios of casein and sucrose was prepared. The mixing ratio of the jelly-type artificial feed is shown in Table 1.

Unit: g comparison group Experimental group 1 Experimental group 2 Experimental group 3 Experimental group 4 incense 0.2 0.2 0.2 0.2 0.2 sucrose 19.5 13.7 9.8 5.9 0.0 casein 0.0 5.9 9.8 13.7 19.5 sorbic acid 0.1 0.1 0.1 0.1 0.1 Methyl-p-hydroxybenzoic acid 0.1 0.1 0.1 0.1 0.1 Baby 2.0 2.0 2.0 2.0 2.0 Distilled water 78.1 78.1 78.1 78.1 78.1 all 100.0 100.0 100.0 100.0 100.0

Specifically, after mixing agar and distilled water, the mixture was heated to 80°C. Then, casein, sucrose, sorbic acid and methyl-p-hydroxybenzoate were mixed in the mixture according to the mixing ratio of Table 1 to prepare a jelly-type artificial feed. The prepared jelly-type artificial feed was stored at 5 ℃.

As the control jelly, Megabucks brown sugar jelly purchased from the Chungwoo Insect Museum was used.

Example 2. Breeding of white-spotted flower radish using artificial jelly-type feed

Adult female and male white-spotted beetles were prepared in equal proportions. Adult white-spotted flower radish was reared in a cage (Neobox 30, 207 x 140 x 124 cm), and the temperature in the cage was maintained at 25℃. In addition, jelly-type artificial feeds (Example 1) having different casein contents were provided to adults of white spot flower radish, respectively, and were replaced every 2-3 days.

Example 3. Comparison of egg-laying numbers of white-spotted beetles according to casein content in artificial feed

The egg-laying numbers of the white-spotted flower larvae fed the jelly-type artificial feed prepared in Example 1 were compared. Specifically, the number of spawning of the white-spotted genus oleifera adults reared in Example 2 was measured weekly, and the results are shown in FIG. 1 .

As shown in FIG. 1 , in experimental groups 1 to 3, it was confirmed that the number of eggs laid by adults of white-spotted chrysanthemum was higher than that of the control group. In particular, in Experimental Group 1, it was confirmed that the number of spawning of the white-spotted larvae fed the artificial jelly-type food was increased by about 2.2 times compared to the control group.

Example 4. Comparison of spawning period of white-spotted beetles according to casein content in artificial feed

The egg-laying period of the white-spotted flower larvae fed the jelly-type artificial feed prepared in Example 1 was compared. Specifically, the spawning period of the white-spotted beetle larvae reared in Example 2 was confirmed by counting the number of eggs laid at weekly intervals until the adults died. The results of confirming the spawning period of the adult white-spotted chrysanthemum are shown in FIGS. 2 and 3 .

As shown in FIGS. 2 and 3 , when the jelly-type artificial feed of experimental groups 1 and 2 was provided, it was confirmed that the spawning period and the number of spawning were increased in the number of spawning of the white-spotted beetles compared to the control group. In particular, it can be seen that the spawning period of the white-spotted larvae fed the jelly-type artificial feed of Experimental Group 1 was 10.3 weeks, which was increased by about 1.5 times compared to the control group.

Example 5. Comparison of survival period of white-spotted white spot beetles according to casein content in artificial feed

The survival period of the white-spotted flower larvae fed the jelly-type artificial feed prepared in Example 1 was compared. Specifically, survival was investigated every week until the adult larvae bred in Example 2 died, and the results are shown in FIG. 4 .

As shown in FIG. 4 , it was confirmed that the survival period of the adult white-spotted chrysanthemum larvae fed the jelly-type artificial feed of Experimental Group 1 was 229.6 days for males and 245.0 days for females. The above results mean that the jelly-type artificial feed of Experimental Group 1 significantly increased the survival period of the white-spotted flower beetle than the jelly-type artificial feed of other mixing ratios.

Overall, the present inventors prepared a jelly-type artificial feed optimized for white spot flower radish, and confirmed that the number of spawning, spawning period and survival period of white spot flower radish were significantly increased when the prepared artificial feed was fed. This means that the jelly-type artificial feed according to the present invention can be usefully used in breeding white-spotted flower radish, and it can be used in various ways in the field of insect breeding, furthermore, in the pharmaceutical and food fields.

As mentioned above, a specific part of the present invention has been described in detail, and for those of ordinary skill in the art, it is obvious that this specific technique is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

With respect to 100 parts by weight of the composition,
An artificial feed composition for breeding white spot flower radish, comprising 3 to 15 parts by weight of casein. The method of claim 1,
The composition further comprises at least one sugar source selected from the group consisting of sucrose, maltose, lactose, lactulose, trehalose, cellobiose and chitobiose, an artificial feed composition for breeding white spot flower radish. The method of claim 2,
The composition has a weight ratio of casein and sugar source of 7:3 to 3:7, an artificial feed composition for breeding white spot flower radish. The method of claim 1,
The composition further comprises at least one selected from the group consisting of sorbic acid, methyl-p-hydroxybenzoic acid, and agar. Preparing a mixture by adding 0.5 to 3 parts by weight of agar and 3 to 15 parts by weight of casein with respect to 100 parts by weight of the mixture; Containing, a method for producing artificial feed for breeding white spot flower radish. The method of claim 5,
In the above step, one or more sugar sources selected from the group consisting of sucrose, maltose, lactose, lactulose, trehalose, cellobiose and chitobiose are further added to the mixture. The method of claim 6,
In the above step, the weight ratio of casein and sugar source is 7:3 to 3:7, the method for producing artificial feed for breeding white spot flower radish. The method of claim 5,
In the above step, sorbic acid or methyl-p-hydroxybenzoic acid is further added to the mixture. The method of claim 8,
The step is based on 100 parts by weight of the mixture,
A method for producing artificial feed for breeding white spot flower radish, further adding 0.05 to 1.5 parts by weight of sorbic acid and 0.05 to 1.5 parts by weight of methyl-p-hydroxybenzoic acid. [Claim 5] An artificial breeding method of white-spotted flower radish, comprising the step of feeding the white-spotted flower radish according to any one of claims 1 to 4.

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