KR20210033137A - Manufacturing Method of Chlorella with the effect Preventing Cold Weather Damage and Root Promoting - Google Patents

Abstract

The present invention relates to a method for preparing Chlorella with an excellent effect of preventing cold weather damage and promoting rooting. The method comprises the following steps: pre-treating groundwater (step 1); preparing a chlorella culture fluid by mixing potassium dihydrogen phosphate and vinegar in the pretreated groundwater (step 2); proliferating chlorella seeds after introducing the chlorella culture fluid into a water storage tank (step 3); collecting a chlorella stock solution through a centrifuge at 5,000 to 20,000 RPM upon the end of the proliferation of the chlorella seeds, and then removing moisture at a predetermined degree or more to prepare chlorella green spots (step 4); and diluting the chlorella green spots with the chlorella stock solution to prepare a chlorella concentrate having a concentration of 1 × 10^7 to 1 × 10^10 pcs/ml (step 5). The method of the present invention improves an effect of preventing cold weather damage and promoting rooting and provides an advantage of shortening a culture period.

Description

Manufacturing Method of Chlorella with the effect Preventing Cold Weather Damage and Root Promoting}

The invention to be coming from a method of manufacturing a cold weather protection and root rooting effect is excellent chlorella, and more particularly, can improve the cold weather protection and root rooting effect, protection that can shorten the culture period, cold weather and roots rooted It relates to a method for producing chlorella excellent in effect.

Chlorella refers to an organism that survives in various ecosystems such as freshwater, seawater, air, and soil, and has chlorophyll, so that it obtains energy necessary for photosynthesis from sunlight and can grow independently by obtaining a carbon source from inorganic substances such as carbon dioxide.

Chlorella has no flagella, so there is no motility, and the cells consist of one nucleus and cup-shaped chloroplast, and the grown cells proliferate rapidly through unusual cell division, and usually four daughter cells form endospores, and Pyrenoidosa ( Pyrenoidosa), Bulgaris, Ellipsoidea, etc. About 10 species are currently known.

This chlorella not only contains all 40 nutrients, including the five major nutrients, but also has excellent photosynthetic ability, so it has 10 times more chlorophyll than ordinary vegetables, and contains a large amount of minerals and vitamins, which are lacking in modern people. It balances the nutrition of the body, and has excellent effects on strengthening immunity, releasing heavy metals, and inhibiting the absorption of dioxin. In addition, as an alkaline food, chlorella has a useful function of maintaining the balance of ionic balance in the body by changing the acidic constitution due to excessive intake of meat or grains into an alkaline constitution.

Such chlorella contains various influencing ingredients depending on the type and culture method, and chlorella with different functions can be produced by different culture methods. In addition, chlorella can be produced in an artificial medium by varying the components of the medium, and recently, chlorella having many growth promoting factors has been produced as a characteristic of the culture method.

Conventionally, in the case of manufacturing chlorella, chlorella seeds were added to a culture medium based on meticulously managed sterile fresh water to proliferate. As a result, the process of managing Clerella seeds and fresh water was inevitably difficult, and it was difficult to supply various nutrients including carbon dioxide, which is the carbon nutrient of chlorella, and to mix them quickly. This acted as a major obstacle to mass production of high-quality chlorella.

In order to solve the above problems, the present applicant has applied for and registered a chlorella manufacturing apparatus. (Korean Registered Patent Publication No. 10-1963415 (2019.03.29))

The chlorella manufacturing apparatus maximizes the contact between the culture medium and the air in a state in which the amount of sunlight is abundantly secured in the greenhouse, thereby inducing hybridization with the chlorella seeds floating in the air, and at the same time actively capturing carbon dioxide, which is a nutrient source in the air. There is an advantage of mass production of chlorella, which has high nutritional value, but there is a disadvantage that the effect of preventing cold damage and rooting is insufficient, and it takes a long period of time to cultivate.

KR 10-1963415 B1 2019.03.29.

It is an object of the present invention to provide a method for producing chlorella excellent in preventing cold damage and rooting effect, which can improve the effect of preventing cold damage and rooting, and shortening the cultivation period.

In order to achieve the above object, the present invention provides the following means.

The present invention, the step of pretreating groundwater (step 1); Mixing potassium dihydrogen phosphate and vinegar to the pretreated groundwater to prepare a chlorella culture solution (step 2); Adding the chlorella culture solution to a storage tank and then proliferating chlorella seeds (step 3); When the proliferation of the chlorella seeds is completed, the chlorella stock solution is collected at an RPM of 5,000 to 20,000 using a centrifuge, and then moisture is removed over a certain amount to prepare chlorella green spots (step 4); And diluting the chlorella green spot with a chlorella stock solution to prepare a chlorella concentrate having a concentration of ^{1×10 7} to 1×10 ^{10 pieces/ml (step 5).} It provides a method for producing chlorella excellent in preventing cold damage and rooting effect, including.

In the step 1, microwaves are irradiated to the mixed water at an output of 130 to 140 kw for 5 to 10 minutes.

In step 2, when preparing the chlorella culture solution, dipotassium hydrogen phosphate (K ₂ HPO ₄ ) 5 to 30 parts by weight, potassium silicate 5 to 30 parts by weight, glucose 10 to 20 parts by weight, sulfuric acid to 100 parts by weight of the pretreated groundwater Magnesium (MgSO ₄ ) 5 to 30 parts by weight and 0.1 to 0.3 parts by weight of raw makgeolli are additionally included.

In the step 2, when preparing the chlorella culture solution, 0.1 to 1 part by weight of lanulin, 0.1 to 1 part by weight of sea squirt extract and 0.1 to 1 part by weight of artichoke leaf extract are additionally included in 100 parts by weight of the pretreated groundwater, wherein Sea sea squirt extract is obtained by adding 300 to 400 parts by weight of water to 100 parts by weight of sea squirt skin, heating at 120 to 130°C for 3 to 4 hours, and then filtering, and the artichoke leaf extract is obtained by filtration with water 300 to 100 parts by weight of artichoke leaves. It is obtained by adding ~400 parts by weight, heating at 100 ~ 110 ℃ for 1 ~ 2 hours, and then filtering.

A carbon dioxide supply device for supplying carbon dioxide to the chlorella culture solution in the storage tank is additionally provided, and an LED light-emitting device for irradiating light to the chlorella culture solution in the storage tank at night is additionally provided.

The manufacturing method of chlorella having excellent anti-cooling effect and root rooting effect according to the present invention can improve the effect of preventing cold damage and root rooting, and has the advantage of shortening the cultivation period.

Hereinafter, the present invention will be described in detail.

The present applicant has applied for and registered a chlorella manufacturing apparatus. (KR 10-1963415 2019.03.29.)

The chlorella production apparatus is installed in a greenhouse and includes a growth unit for proliferating chlorella seeds while storing a chlorella culture solution, wherein the growth unit stores a chlorella culture solution for growing chlorella seeds, but the storage tank in the shape of a container with an open top; One end is connected to the storage tank and the other end is installed to face the open upper side of the storage tank so that a part of the culture solution stored in the storage tank is circulated and sprayed into the air inside the greenhouse so that a part of the culture solution can be recovered by contacting the air. One circulatory duct; And a pump installed in the circulation pipe to provide power for circulating a portion of the culture medium.

The present invention is characterized by providing a method for producing chlorella, which can improve the chlorella production apparatus to prevent cold damage and improve the root rooting effect, and shorten the cultivation period.

First, a method of manufacturing chlorella having excellent anti-cooling effect and root rooting effect according to the present invention will be described.

The manufacturing method of chlorella excellent in preventing cold damage and rooting effect according to the present invention,

Pretreating groundwater (step 1);

Mixing potassium dihydrogen phosphate and vinegar to the pretreated groundwater to prepare a chlorella culture solution (step 2);

Adding the chlorella culture solution to a storage tank and then proliferating chlorella seeds (step 3);

When the proliferation of the chlorella seeds is completed, the chlorella stock solution is collected at an RPM of 5,000 to 20,000 using a centrifuge, and then moisture is removed over a certain amount to prepare chlorella green spots (step 4); And

Diluting the chlorella green spot with a chlorella stock solution to prepare a chlorella concentrate having a concentration of ^{1×10 7} to 1×10 ^{10 pieces/ml (step 5);}

Includes.

Step 1 is a step of irradiating the groundwater with a microwave output of 130 to 140 kw for 5 to 10 minutes.

The present invention is characterized in that the cultivation period is shortened by irradiating the groundwater with microwaves to activate chlorella seeds in the groundwater.

Since most of the groundwater obtained in nature itself contains chlorella seeds, even without using chlorella seeds, a greater number of chlorella seeds are produced by hybridization between the chlorella seeds contained in the groundwater and the chlorella seeds contained in the air. It is possible to multiply this to produce chlorella in large quantities.

The step 2 is a step of preparing a chlorella culture solution by mixing 5 to 30 parts by weight of _{potassium dihydrogen phosphate (KH 2} PO ₄ ) and 0.1 to 0.3 parts by weight of vinegar to 100 parts by weight of the pretreated groundwater.

The potassium dihydrogen phosphate refers to a water-soluble salt frequently used as a fertilizer, food additive, buffer, etc. as a compound made starting with phosphorus and potassium, and is called monobasic potassium phosphate. In addition, it is preferable to use brown rice vinegar naturally fermented by adding yeast and water to rice cooked with brown rice.

When preparing the chlorella culture, 100 parts by weight of the pretreated groundwater dipotassium hydrogen phosphate (K ₂ HPO ₄ ) 5 to 30 parts by weight, potassium silicate 5 to 30 parts by weight, glucose 10 to 20 parts by weight, magnesium sulfate (MgSO ₄ ) 5 to 30 parts by weight and 0.1 to 0.3 parts by weight of raw makgeolli may be additionally included.

The present invention has the advantage of improving the anti-cooling effect by including dipotassium hydrogen phosphate, potassium silicate, glucose, magnesium sulfate, and raw makgeolli containing yeast in the pretreated groundwater.

The potassium hydrogen phosphate is called dibasic potassium phosphate. The potassium hydrogen phosphate serves to increase the protein content in chlorella.

The potassium silicate is a colorless or slightly colored viscous alkaline liquid, used as a soap blending agent, bleaching agent, detergent, etc., in solubility, insoluble in alcohol, but soluble in water. It's easy.

When preparing the chlorella culture solution, 0.1 to 1 part by weight of ranulin, 0.1 to 1 part by weight of sea squirrel extract, and 0.1 to 1 part by weight of artichoke leaf extract may additionally be included in 100 parts by weight of the pretreated groundwater.

Lanolin is an animal oil extracted from sheep's fur.

The sea squirt ( Halocynthia roretzi ) is a marine organism belonging to the seaweed river of the chordal fauna, and inhabits by attaching to rocks and seaweed in sea areas around the world. In particular, in East Asian regions such as Korea and Japan, sea squirt farming has developed and is actively consumed as aquatic food. Sea squirt skin is composed of 43% cellulose and 40% protein.

The sea squirt extract is obtained by adding 300 to 400 parts by weight of water to 100 parts by weight of sea squirt, heating at 120 to 130°C for 3 to 4 hours, and filtering.

The artichoke ( Cynara scolymus ) is a thistle-like perennial flower belonging to the Asteraceae family and is said to have been cultivated since ancient Greece and Roman times and used for food. It is native to the Mediterranean coast and inhabits near the seashore. Unlike other vegetables, large buds are used for food. The artichoke is highly regarded for medicinal use because it contains saccharides that act like insulin, and it is known that leaves or roots have a cholesterol-lowering effect. When used as tea, it helps in diuresis and liver improvement. In addition, it has been known for its strong antioxidant activity, the reporting function for hepatocytes, the effect of preventing adult diseases such as blood sugar control and the effect of promoting digestion. The flower buds of the artichoke are not only soft in flesh and protein in taste, but also have a lot of nutritional value, and are widely used for medicinal purposes as they contain protein, vitamin A and vitamin C, calcium, iron, phosphorus, sugars, and inurine.

The artichoke leaf extract is obtained by adding 300 to 400 parts by weight of water to 100 parts by weight of artichoke leaves, heating at 100 to 110° C. for 1 to 2 hours, and filtering.

The present invention has the advantage of improving the root rooting effect by including lanulin, sea squirt bark extract and artichoke leaf extract in the pretreated groundwater.

Step 3 is a step of proliferating chlorella seeds for 3 days at 25 to 35° C. after the chlorella culture solution is added to a storage tank.

The storage tank stores chlorella culture solution in a greenhouse through which sunlight passes, and is formed in the shape of a container with an open top. Here, the storage tank must be provided with an open top, which is to allow the stored culture solution to be exposed as it is to the air in the greenhouse.

A chlorella culture solution supply pipe required for supplying the chlorella culture solution is connected to the storage tank, and a circulation pipe that circulates the power of the pump is also provided for some of the stored chlorella culture solution. Here, the circulation pipe is installed so that one end is connected to the lower end of the reservoir and the other end faces the open upper side of the reservoir. As a result, a part of the culture solution stored in the storage tank is circulated and sprayed into the air inside the greenhouse, so that a part of the chlorella culture solution can be recovered by contacting the air. When some of the stored chlorella culture liquid is configured to be recovered after contacting with air, chlorella seeds and carbon dioxide present in the air can be captured and joined to the chlorella culture liquid stored in the storage tank. Then, hybridization between the chlorella seeds captured in the air and the chlorella seeds present in the culture medium is achieved, and the carbon dioxide captured in the air serves as an essential carbon nutrient source for the production and proliferation of chlorella seeds.

Further, the other end of the circulation pipe is bent at the upper side of the storage tank so that the culture solution circulating toward the circumferential portion of the culture solution stored in the storage tank rather than the center portion can be sprayed. According to this configuration, a wave occurs when the culture solution injected in the air first collides with the culture solution stored in the storage tank, and secondly, the culture solution stored in the storage tank rotates in a spiral and vortex. In both, stirring between the culture medium and the chlorella seeds mixed therein is very smooth, and active growth is possible through the uniform distribution of the chlorella seeds. Here, if you simply think about the stirring operation, you can also think of a very general configuration of installing the stirring blades inside the storage tank, but rather to save the cost required for the stirring blades through the above configuration, as well as to maximize the advantages required by the present invention. It has an important meaning in that it was done. In other words, if a part of the culture medium is sprayed into the air and has a stirring function, not only the stirring function but also the contact between the culture medium and the air is induced, so that the chlorella seeds present in the air and carbon dioxide, which is a carbon nutrient source, can be actively captured from the air. It has a technical significance.

A portion of the culture medium is injected into the air using the circulation pipe and the pump and then circulated in a manner of recovering it to maximize the contact between the air and the culture medium. In this way, the culture medium is sprayed into the air so that the mixed water and air contact with each other actively is to induce the hybridization of the chlorella present in the air and the chlorella present in the mixed water, but to capture carbon dioxide, which is a carbon nutrient source, in the air. It is also.

A carbon dioxide supply device for supplying carbon dioxide to the chlorella culture solution in the storage tank may be additionally provided.

The present invention has the advantage of further increasing the chlorella culture rate by additionally providing a carbon dioxide supply device.

An LED light emitting device for irradiating light at night to the chlorella culture solution in the storage tank may be additionally provided.

The present invention has the advantage of further increasing the chlorella culture rate by irradiating light even at night by additionally providing an LED light emitting device.

It is preferable that the pulse period of the LED is 400 to 800 µs.

Step 4 is a step of preparing chlorella green spot by collecting chlorella stock solution at an RPM of 5,000 to 20,000 using a centrifuge when the chlorella seeds are grown, and then removing moisture over a certain amount.

Step 5 is a step of diluting the chlorella green spot with a chlorella stock solution to prepare a chlorella concentrate having a concentration of ^{1×10 7} to 1×10 ^{10 pieces/ml.}

The manufacturing method of chlorella having excellent anti-cooling effect and root rooting effect according to the present invention can improve the effect of preventing cold damage and root rooting, and has the advantage of shortening the cultivation period.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

The groundwater was irradiated with a microwave output of 140 kw for 10 minutes. A chlorella culture solution was prepared by mixing 10 parts by weight of _{potassium dihydrogen phosphate (KH 2} PO ₄ ) and 0.3 parts by weight of vinegar to 100 parts by weight of the pretreated groundwater. After the chlorella culture solution was added to a storage tank, chlorella seeds were grown at 25° C. for 3 days. The storage tank stores the chlorella culture solution in a greenhouse through which sunlight passes, and is formed in a container shape with an open top. The chlorella culture solution was supplied to the storage tank through a chlorella culture solution supply tube. A circulation pipe for circulating some of the stored chlorella culture broth using the power of a pump was installed in the storage tank. The circulation pipe was installed so that one end was connected to the lower end of the water storage tank and the other end faced the open upper side of the water storage tank. The other end of the circulation pipe was bent at the upper side of the storage tank to spray the chlorella culture solution circulating toward the circumferential portion of the culture solution stored in the storage tank rather than the center. When the culture solution injected from the air first collides with the culture solution stored in the storage tank, a wave occurs, and secondly, the culture solution stored in the storage tank rotates in a spiral and vortex. The stirring between the chlorella seeds mixed therein was made very smoothly, and active growth occurred through the uniform distribution of the chlorella seeds. A portion of the culture solution was injected into the air using a circulation pipe and a pump, and then circulated in a manner of recovering it to maximize the contact between the air and the culture solution. By spraying the culture solution into the air, the contact between the groundwater and the air was made active, thereby inducing a hybrid of chlorella existing in the air and chlorella existing in the groundwater, and capturing carbon dioxide, a carbon nutrient source, in the air. Carbon dioxide was additionally supplied to the chlorella culture solution in the storage tank using a carbon dioxide supply device. Light was irradiated at night to the chlorella culture solution in the storage tank using an LED light emitting device. When the proliferation of the chlorella seeds was completed, a chlorella stock solution was collected at RPM of 20,000 using a centrifuge, and then moisture was removed over a certain amount to prepare chlorella green spots. The chlorella green spot was diluted with chlorella stock solution to prepare a chlorella concentrate having a concentration of ^{1×10 9 pieces/ml.}

In Example 1, when preparing the chlorella culture solution, dipotassium hydrogen phosphate (K ₂ HPO ₄ ) 5 parts by weight, potassium silicate 5 parts by weight, glucose 10 parts by weight, magnesium sulfate (MgSO ₄ ) to 100 parts by weight of the pretreated groundwater The rest were prepared in the same manner, except that 5 parts by weight and 0.1 parts by weight of raw makgeolli were additionally included.

[Comparative Example 1]

In Example 1, except for omitting the process of pretreatment of groundwater, omitting additional supply of carbon dioxide to the chlorella culture solution, and omitting light irradiation using an LED light-emitting device at night in the chlorella stock solution. In the same manner, a chlorella concentrate was prepared.

[Experimental Example 1]

Apples were grown in an orchard in Danyang-gun, Chungcheongbuk-do, where apple cold damage occurs. Around the beginning of May 2019, the low temperature lasted for more than 5 days, resulting in short apple blossoms and severe obstacles to falling flowers. The chlorella of Examples 1 and 2 and Comparative Example 1 was diluted 1000 times, and on May 10, 2019, 1 bottle per 200 pyeong was first treated as a drench, and then a total of 4 times were drenched at 15-day intervals, and the results were obtained. Was observed, and the results are shown in Table 1.

Fall rate (%) Fruiting rate (%) Fruit weight after harvest (g) Example 1 7.1 92.9 441.6 Example 2 8.4 91.6 434.5 1 in comparison 10.5 89.5 427.8

According to Table 1, it can be seen that the chlorella of Example 1 and Example 2 is superior to the chlorella of Comparative Example 1 to prevent cold damage.

In Example 1, when preparing the chlorella culture solution, the rest were prepared in the same manner, except that 1 part by weight of lanulin, 1 part by weight of sea squirt extract and 1 part by weight of artichoke leaf extract were additionally included in 100 parts by weight of the pretreated groundwater. . The sea squirt extract was obtained by adding 400 parts by weight of water to 100 parts by weight of sea squirt, heating at 130° C. for 4 hours, and filtering. The artichoke leaf extract was obtained by adding 400 parts by weight of water to 100 parts by weight of artichoke leaves, heating at 100° C. for 1 hour, and filtering.

[Experimental Example 2]

The chlorella of Examples 1, 3 and Comparative Example 1 was diluted 200 times to fertilize tomatoes three times at two-week intervals after planting, and the average values of height, root length, leaf number, and weight of tomato plants grown for 7 weeks were measured. It is shown in 2.

Height(cm) Root length (cm) Number of leaves (pcs) Weight(g) Example 1 56.1 18.3 35 11.5 Example 3 58.9 19.4 42 11.9 Comparative Example 1 52.3 14.8 31 8.3

According to Table 2, it can be seen that the chlorella of Example 1 and Example 3 has superior root rooting effect compared to the chlorella of Comparative Example 1.

Claims (5)

Pretreating groundwater (step 1);
Mixing potassium dihydrogen phosphate and vinegar to the pretreated groundwater to prepare a chlorella culture solution (step 2);
Adding the chlorella culture solution to a storage tank and then proliferating chlorella seeds (step 3);
When the proliferation of the chlorella seeds is completed, the chlorella stock solution is collected at an RPM of 5,000 to 20,000 using a centrifuge, and then moisture is removed over a certain amount to prepare chlorella green spot (step 4); And
Diluting the chlorella green spot with a chlorella stock solution to prepare a chlorella concentrate having a concentration of ^{1×10 7} to 1×10 ^{10 pieces/ml (step 5);}
Containing,
Method for producing chlorella excellent in preventing cold damage and rooting effect.
The method of claim 1,
The step 1 is to irradiate the mixed water with a microwave output of 130 to 140 kw for 5 to 10 minutes,
Method for producing chlorella excellent in preventing cold damage and rooting effect.
The method of claim 1, wherein in step 2,
When preparing the chlorella culture, 100 parts by weight of the pretreated groundwater dipotassium hydrogen phosphate (K ₂ HPO ₄ ) 5 to 30 parts by weight, potassium silicate 5 to 30 parts by weight, glucose 10 to 20 parts by weight, magnesium sulfate (MgSO ₄ ) 5 to 30 parts by weight and 0.1 to 0.3 parts by weight of raw makgeolli additionally,
Method for producing chlorella excellent in preventing cold damage and rooting effect.
The method of claim 1, wherein in step 2,
When preparing the chlorella culture solution, 0.1 to 1 part by weight of ranulin, 0.1 to 1 part by weight of sea squirt extract and 0.1 to 1 part by weight of artichoke leaf extract are additionally included in 100 parts by weight of the pretreated groundwater,
The sea squirt extract is obtained by adding 300 to 400 parts by weight of water to 100 parts by weight of sea squirt, heating at 120 to 130°C for 3 to 4 hours, and then filtering,
The artichoke leaf extract is obtained by filtration after adding 300 to 400 parts by weight of water to 100 parts by weight of artichoke leaves, heating at 100 to 110° C. for 1 to 2 hours,
Method for producing chlorella excellent in preventing cold damage and rooting effect.
The method of claim 1,
A carbon dioxide supply device for supplying carbon dioxide to the chlorella culture solution in the storage tank is additionally provided,
An LED light emitting device for irradiating light at night to the chlorella culture solution in the storage tank is additionally provided,
Method for producing chlorella excellent in preventing cold damage and rooting effect.