KR20210031926A

KR20210031926A - 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체 효소

Info

Publication number: KR20210031926A
Application number: KR1020217003700A
Authority: KR
Inventors: 스콧 제이 노빅; 니키 델라스; 베스나 미첼; 다 두안; 요바나 나조르; 오스카 알비조; 오릭 앤서니 소웰-칸츠; 제프리 씨 무어; 마크 허프만; 아구스티나 로드리게즈-그라닐로; 딥탁 베르마; 니콜라스 엠 마샬; 제이 러쎌; 키이스 에이 카나다
Original assignee: 코덱시스, 인코포레이티드
Priority date: 2018-07-09
Filing date: 2019-07-02
Publication date: 2021-03-23
Also published as: JP2023126615A; MX2021000327A; CN112601543A; US20200010834A1; JP2021530979A; US11162105B2; JP7461658B2; EP3820502A4; CA3103718A1; SG11202012111UA; EP3820502A1; US12110493B2; WO2020014047A1; IL279886A; AU2019302423A1; US20220010316A1

Abstract

본 발명은 조작된 퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소, PNP 활성을 갖는 폴리펩타이드, 및 이들 효소를 코딩하는 폴리뉴클레오타이드뿐만 아니라, 이들 폴리뉴클레오타이드 및 폴리펩타이드를 포함하는 벡터 및 숙주 세포를 제공한다. PNP 효소를 생산하는 방법도 또한 제공된다. 본 발명은 추가로 PNP 효소를 포함하는 조성물 및 조작된 PNP 효소를 사용하는 방법을 제공한다. 본 발명은 약제학적 화합물의 생산에서 특별한 용도를 제시한다.

Description

조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체 효소

본 출원은 2018년 7월 9일 출원된 미국 특허 가출원 제62/695,507호 및 2019년 3월 22일 출원된 미국 특허 가출원 제62/822,263호에 기초한 우선권을 주장하고, 상기 두 출원은 모든 목적을 위해 그 전체가 참조로 포함된다.

발명의 분야

서열 목록, 표 또는 컴퓨터 프로그램에 대한 언급

서열 목록의 공식 사본은 EFS-Web을 통해 ASCII 형식의 텍스트 파일로 본 명세서와 동시에 제출되고, 파일 이름은 "CX2-172WO1_ST25.txt"이고, 그 작성일은 2019년 6월 25일이며, 파일 크기는 1,547 킬로바이트이다. EFS-Web을 통해 제출된 서열 목록은 명세서의 일부이며, 그 전체가 본 명세서에 참조로 포함된다.

인간 면역 결핍 바이러스(HIV)로 지정된 레트로바이러스는 이환된 개체의 면역계의 점진적인 파괴 및 중추 및 말초 신경계의 퇴화를 수반하는 복합 질환인 후천성 면역 결핍 증후군(AIDS)의 병인체이다. 레트로바이러스 복제의 일반적인 특징은 바이러스 복제에 필요한 HIV 서열의 DNA 사본을 생성하기 위해 바이러스에 의해 코딩되는 역전사 효소에 의한 바이러스 RNA 게놈의 역전사이다. MK-8591과 같은 일부 화합물은 역전사 효소 억제제로 알려져 있으며, AIDS 및 유사한 질환의 치료에 유용한 것으로 밝혀졌다. HIV 역전사 효소를 억제하는 것으로 알려진 일부 화합물이 있지만, 상기 효소를 억제하는데 보다 효과적이고 따라서 AIDS의 효과를 개선하는 추가의 화합물에 대한 필요성이 관련 기술 분야에 계속 존재한다.

MK-8591(Merck)과 같은 뉴클레오사이드 유사체는 DNA 합성에 사용되는 천연 뉴클레오사이드와의 유사성 때문에 HIV의 역전사 효소에 대한 효과적인 억제제이다. 역전사 효소에 의한 이러한 유사체의 결합은 역전사 효소의 점진적 특성을 억제함으로써 DNA 합성을 정지시킨다. 효소의 정지는 DNA 분자의 조기 종결을 초래하여 상기 분자를 효과적이지 못하도록 만든다. 그러나, 표준 화학 합성 기술에 의한 뉴클레오사이드 유사체의 생산은 이들의 화학적 복잡성으로 인해 문제를 제기할 수 있다.

발명의 요약

본 발명은 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제를 제공하고, 여기서 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 참조하여 넘버링된다. 일부 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는다. 일부 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

207, 및 212로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 참조하여 넘버링된다.

본 발명은 서열 번호 6에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제를 제공하고, 여기서 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 6을 참조하여 넘버링된다. 일부 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 6에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 6을 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 6을 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 6을 참조하여 넘버링된다.

본 발명은 서열 번호 126에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제를 제공하고, 여기서 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 126을 참조하여 넘버링된다. 일부 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 126에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 126을 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

216Y, 및 220F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 126을 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

및 T220F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 126을 참조하여 넘버링된다.

본 발명은 서열 번호 242에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제를 제공하고, 여기서 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 242를 참조하여 넘버링된다. 일부 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 242에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 242를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 242를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 242를 참조하여 넘버링된다.

본 발명은 서열 번호 684에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제를 제공하고, 여기서 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 684를 참조하여 넘버링된다. 일부 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 684에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 10, 10/133/227, 10/145, 10/145/227, 18, 20, 26, 29, 39/98, 39/133/227, 60, 62, 63, 74, 89, 94, 97, 97/98, 108, 126, 133, 135, 145, 161, 162, 165, 167, 168, 177, 199, 208, 210, 212, 224, 및 227로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 684를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 10P,

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 684를 참조하여 넘버링된다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 M10P,

로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 684를 참조하여 넘버링된다.

일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 표 4.1, 5.1, 6.1, 7.1 및/또는 8.1에 제시된 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 4-1002의 짝수 번호 서열에 제시된 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 서열 번호 2-1002의 짝수 번호 서열 중 적어도 하나에 제시된 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 야생형 이. 콜라이(E. coli) 퓨린 뉴클레오사이드 포스포릴라제에 비해 적어도 하나의 개선된 특성을 포함한다. 일부 추가의 실시양태에서, 개선된 특성은 기질에 대한 개선된 활성을 포함한다. 일부 실시양태에서, 기질은 화합물 4를 포함한다. 일부 실시양태에서, 기질은 화합물 3을 포함한다. 일부 실시양태에서, 기질은 화합물 8을 포함한다. 일부 추가의 실시양태에서, 개선된 특성은 화합물 1의 개선된 생산을 포함한다. 일부 추가의 실시양태에서, 개선된 특성은 화합물 10의 개선된 생산을 포함한다. 또 다른 일부 추가의 실시양태에서, 조작된 퓨린 뉴클레오사이드 포스포릴라제는 정제된다. 본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 포함하는 조성물을 제공한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 포함한다.

본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 코딩하는 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드 서열은 서열 번호 1, 5, 125, 241, 및/또는 683에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 포함한다. 일부 추가의 실시양태에서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리뉴클레오타이드 서열은 하나 이상의 위치에 적어도 하나의 치환을 포함한다. 일부 추가의 실시양태에서, 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 코딩하는 폴리뉴클레오타이드 서열은 서열 번호 1, 5, 125, 241, 및/또는 683, 또는 그의 기능적 단편에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리뉴클레오타이드 서열을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 제어 서열에 작동 가능하게 연결된다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 코돈 최적화된다. 일부 추가의 실시양태에서, 폴리뉴클레오타이드 서열은 서열 번호 3-1001의 홀수 번호 서열에 제시된 폴리뉴클레오타이드 서열을 포함한다.

본 발명은 또한 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 발현 벡터를 제공한다. 본 발명은 추가로 본원에서 제공되는 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 숙주 세포는 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열을 포함한다. 본 발명은 또한 숙주 세포에서 조작된 퓨린 뉴클레오사이드 포스포릴라제를 생산하는 방법을 제공하며, 이 방법은 본원에서 제공되는 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제가 생산되도록 적합한 조건 하에서 숙주 세포를 배양하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 배양액 및/또는 숙주 세포로부터 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 회수하는 단계를 추가로 포함한다. 일부 추가의 실시양태에서, 상기 방법은 상기 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 정제하는 단계를 추가로 포함한다.

발명의 설명

달리 정의되지 않는 한, 본원에서 사용되는 모든 기술 및 과학 용어는 일반적으로 본 발명이 속하는 기술 분야에서 통상의 기술을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 갖는다. 일반적으로, 본원에서 사용 명명법 및 아래에서 설명되는 세포 배양, 분자 유전학, 미생물학, 유기 화학, 분석 화학 및 핵산 화학의 실험 절차는 관련 기술 분야에 널리 공지되고 일반적으로 사용되는 것이다. 이러한 기술은 널리 공지되어 있고 관련 기술 분야에서 통상의 기술을 가진 자에게 널리 알려진 많은 교과서 및 참고서에 기재되어 있다. 표준 기술, 또는 이의 변형이 화학적 합성 및 화학적 분석에 사용된다. 상기 및 아래에서 언급되는 모든 특허, 특허 출원, 논문 및 간행물은 본원에서 참고로 명시적으로 포함된다.

본원에서 설명되는 것과 유사하거나 대등한 임의의 적합한 방법 및 물질이 본 발명의 실시에 사용되지만, 일부 방법 및 물질이 본원에서 설명된다. 본 발명은 기재된 특정 방법론, 프로토콜, 및 시약으로 한정되지 않는 것으로 이해되어야 하는데, 이들은 이들이 관련 기술 분야에서 통상의 기술을 가진 자에 의해 사용되는 맥락에 따라 달라질 수 있기 때문이다. 따라서, 바로 아래에 정의된 용어는 본 발명을 전체로서 참조하여 보다 완전하게 설명된다.

전술한 일반적인 설명과 하기의 상세한 설명은 모두 단지 예시적이고 설명하기 위한 것으로서, 본 발명을 제한하는 것이 아닌 것으로 이해되어야 한다. 본원에서 사용되는 섹션 제목은 단지 그 구성을 조직적으로 제시하기 위한 것이며 기술된 주제를 제한하는 것으로 해석되지 않아야 한다. 수치 범위는 범위를 한정하는 수를 포함한다. 따라서, 본원에 개시되는 모든 수치 범위는, 마치 더 좁은 수치 범위가 모두 본원에 명시적으로 기재된 것처럼, 이러한 더 넓은 수치 범위 내에 속하는 모든 더 좁은 수치 범위를 포함하는 것으로 의도된다. 또한, 본원에서 개시되는 모든 최대(또는 최소) 수치 제한은, 마치 더 낮은(또는 더 높은) 수치 제한이 본원에 명시적으로 기재된 것처럼, 모든 더 낮은(또는 더 높은) 수치 한정을 포함하는 것으로 의도된다.

약어 및 정의

유전자에 의해 코딩되는 아미노산에 대해 사용되는 약어는 통상적이고, 다음과 같다: 알라닌(Ala 또는 A), 아르기닌(Are 또는 R), 아스파라긴(Asn 또는 N), 아스파르테이트(Asp 또는 D), 시스테인(Cys 또는 C), 글루타메이트(Glu 또는 E), 글루타민(Gln 또는 Q), 히스티딘(His 또는 H), 이소류신(Ile 또는 I), 류신(Leu 또는 L), 라이신(Lys 또는 K), 메티오닌(Met 또는 M), 페닐알라닌(Phe 또는 F), 프롤린(Pro 또는 P), 세린(Ser 또는 S), 트레오닌(Thr 또는 T), 트립토판(Trp 또는 W), 티로신(Tyr 또는 Y) 및 발린(Val 또는 V).

세 글자 약어가 사용되는 경우, 명시적으로 "L" 또는 "D"가 앞에 나오지 않거나 또는 약어가 사용된 문맥으로부터 명확하지 않는 한, 아미노산은 α-탄소(Cα)에 대해 L- 또는 D-배열일 수 있다. 예를 들어, "Ala"는 α-탄소에 대한 배열을 명시하지 않은 알라닌을 나타내고, "D-Ala" 및 "L-Ala"는 각각 D-알라닌 및 L-알라닌을 나타낸다. 한 글자 약어가 사용되는 경우, 대문자는 α-탄소에 대한 L-배열의 아미노산을 나타내고, 소문자는 α-탄소에 대한 D-배열의 아미노산을 나타낸다. 예를 들어, "A"는 L-알라닌을 나타내고, "a"는 D-알라닌을 나타낸다. 폴리펩타이드 서열이 한 줄의 한 글자 또는 세 글자 약어(또는 이들의 혼합)로 제시될 때, 서열은 통상적인 관례에 따라 아미노(N)에서 카르복시(C) 방향으로 제시된다.

유전자에 의해 코딩되는 뉴클레오사이드에 대해 사용된 약어는 통상적이며, 다음과 같다: 아데노신(A); 구아노신(G); 시티딘(C); 티미딘(T); 및 우리딘(U). 명시적으로 서술되지 않는 한, 약칭된 뉴클레오사이드는 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드일 수 있다. 뉴클레오사이드는 개별적인 기준으로 또는 종합적인 기준으로 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드인 것으로 특정될 수 있다. 핵산 서열이 한 줄의 한 글자 약어로 제시될 때, 서열은 통상적인 관례에 따라 5'에서 3' 방향으로 제시되고, 포스페이트는 표시되지 않는다.

본 발명과 관련하여, 본원의 설명에 사용되는 기술 및 과학 용어는, 달리 명시적으로 정의되지 않는 한, 관련 기술 분야에서 통상의 기술을 가진 자에 의해 통상적으로 이해되는 의미를 가질 것이다. 따라서, 하기 용어는 하기 의미를 갖는 것으로 의도된다.

본원에 사용되는 바와 같이, 단수 형태 "a", "an" 및 "the"는 문맥이 달리 명확히 나타내지 않는 한, 복수의 대상을 포함한다. 따라서, 예를 들어, "폴리펩타이드"에 대한 언급은 둘 이상의 폴리펩타이드를 포함한다.

이와 유사하게, "포함하다", "포함하는", "포괄하다", 및 " 포괄하는"은 상호 교환 가능하며, 제한하고자 하는 것은 아니다. 따라서, 본원에서 사용되는 바와 같이, 용어 "포함하는" 및 그의 동족어는 포괄적인 의미로 사용된다(즉, 용어 "포괄하는" 및 그의 상응하는 동족어와 동등함).

다양한 실시양태의 설명이 용어 "포함하는"을 사용하는 경우, 관련 기술 분야에서 통상의 기술을 가진 자는 일부 특정 경우에, 실시양태가 표현 "본질적으로 이루어지는" 또는 "이루어지는"을 사용하여 대안적으로 설명될 수 있음을 이해할 것임이 추가로 이해되어야 한다.

본원에서 사용되는 바와 같이, 용어 "약"은 특정 값에 대한 허용 가능한 오차를 의미한다. 일부 경우에, "약"은 주어진 값 범위의 0.05%, 0.5%, 1.0%, 또는 2.0% 이내를 의미한다. 일부 경우에, "약"은 주어진 값의 1, 2, 3, 또는 4 표준 편차 이내를 의미한다.

본원에서 사용되는 바와 같이, "EC" 번호는 생화학 및 분자 생물학의 국제 연합의 명명 위원회(NC-IUBMB)의 효소 명명법을 지칭한다. IUBMB 생화학 분류는 효소가 촉매작용을 하는 화학적 반응에 기초한, 효소에 대한 수치적 분류 시스템이다.

본원에서 사용되는 바와 같이, "ATCC"는 그의 생체 시료 저장(biorepository) 수집물이 유전자 및 균주를 포함하는 아메리칸 타입 컬쳐 컬렉션(American Type Culture Collection)을 지칭한다.

본원에서 사용되는 바와 같이, "NCBI"는 미국 국립 생물 정보 센터(National Center for Biological Information) 및 그 안에 제공된 서열 데이터베이스를 지칭한다.

본원에서 사용되는 바와 같이, "포스포펜토뮤타제"("PPM") 효소는 리보스 1-포스페이트의 리보스 5-포스페이트 및 관련 화합물, 예를 들어 데옥시리보스 포스페이트 및 리보스 포스페이트 및 데옥시리보스 포스페이트의 유사체로의 가역적 이성질체화를 촉매하는 효소이다.

본원에서 사용되는 바와 같이, "퓨린 뉴클레오사이드 포스포릴라제"("PNP") 효소는 퓨린 리보뉴클레오사이드 및 관련 화합물(예를 들어, 데옥시리보뉴클레오사이드 및 리보뉴클레오사이드 및 데옥시리보뉴클레오사이드의 유사체)의 유리 퓨린 염기 및 리보스-1-포스페이트(및 이의 유사체)로의 가역적 인산화를 촉매하는 효소이다. "단백질", "폴리펩타이드" 및 "펩타이드"는, 길이 또는 번역후 변형(예를 들어, 글리코실화 또는 인산화)과 관계없이, 아미드 결합에 의해 공유 결합된 적어도 2개의 아미노산의 중합체를 지칭하기 위해 본원에서 상호 교환 가능하게 사용된다. 이 정의에는 D- 및 L-아미노산, 및 D-아미노산과 L-아미노산의 혼합물뿐만 아니라, D- 및 L-아미노산을 포함하는 중합체, 및 D-아미노산과 L-아미노산의 혼합물이 포함된다.

"아미노산"은 이들의 통상적으로 공지된 세 글자 기호에 의해 또는 IUPAC-IUB 생화학적 명명 심의회(Biochemical Nomenclature Commission)에 의해 권고된 한 글자 기호에 의해 본원에서 언급된다. 마찬가지로, 뉴클레오타이드는 이들의 일반적으로 허용되는 단일 글자 코드에 의해 언급될 수 있다.

본원에서 사용되는 바와 같이, "친수성 아미노산 또는 잔기"는 문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]]의 표준화된 합의된 소수성 척도에 따라 0 미만의 소수성을 나타내는 측쇄를 갖는 아미노산 또는 잔기를 의미한다. 유전자에 의해 코딩되는 친수성 아미노산에는 L-Thr(T), L-Ser(S), L-His(H), L-Glu(E), L-Asn(N), L-Gln(Q), L-Asp(D), L-Lys(K) 및 L-Arg(R)이 포함된다.

본원에서 사용되는 바와 같이, "산성 아미노산 또는 잔기"는 아미노산이 펩타이드 또는 폴리펩타이드에 포함될 때 약 6 미만의 pK 값을 나타내는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 산성 아미노산은 통상적으로 수소 이온의 손실로 인해 생리학적 pH에서 음 하전된 측쇄를 갖는다. 유전자에 의해 코딩되는 산성 아미노산에는 L-Glu(E) 및 L-Asp(D)가 포함된다.

본원에서 사용되는 바와 같이, "염기성 아미노산 또는 잔기"는 아미노산이 펩타이드 또는 폴리펩타이드에 포함될 때 약 6 초과의 pK 값을 나타내는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 염기성 아미노산은 통상적으로 히드로늄 이온과의 회합으로 인해 생리학적 pH에서 양 하전된 측쇄를 갖는다. 유전자에 의해 코딩되는 염기성 아미노산에는 L-Arg(R) 및 L-Lys(K)가 포함된다.

본원에서 사용되는 바와 같이, "극성 아미노산 또는 잔기"는 생리학적 pH에서 하전되어 있지 않지만 2개의 원자에 의해 공유된 한 쌍의 전자가 원자 중 1개에 의해 더욱 가까이 유지된 적어도 1개의 결합을 갖는 측쇄를 갖는 친수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 극성 아미노산에는 L-Asn(N), L-Gln(Q), L-Ser(S) 및 L-Thr(T)이 포함된다.

본원에서 사용되는 바와 같이, "소수성 아미노산 또는 잔기"는 문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]]의 표준화된 합의된 소수성 척도에 따라 0 초과의 소수성을 나타내는 측쇄를 갖는 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 소수성 아미노산에는 L-Pro(P), L-Ile(I), L-Phe(F), L-Val(V), L-Leu(L), L-Trp(W), L-Met(M), L-Ala(A) 및 L-Tyr(Y)이 포함된다.

본원에서 사용되는 바와 같이, "방향족 아미노산 또는 잔기"는 적어도 1개의 방향족 또는 헤테로방향족 고리를 포함하는 측쇄를 갖는 친수성 또는 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 방향족 아미노산은 L-Phe(F), L-Tyr(Y) 및 L-Trp(W)를 포함한다. 그의 헤테로방향족 질소 원자 L-His(H)의 pKa로 인해, 이는 때때로 염기성 잔기로서 분류되거나, 이의 측쇄가 헤테로방향족 고리를 포함하기 때문에 방향족 잔기로서 분류되지만, 본원에서는 히스티딘을 친수성 잔기 또는 "구속형(constrained) 잔기"로서 분류한다(하기 참조).

본원에서 사용되는 바와 같이, "구속형 아미노산 또는 잔기"는 구속형 기하형태를 갖는 아미노산 또는 잔기를 지칭한다. 본원에서, 구속형 잔기는 L-pro(P) 및 L-His(H)을 포함한다. 히스티딘은 구속형 기하형태를 갖는데, 이는 이것이 비교적 작은 이미다졸 고리를 갖기 때문이다. 프롤린은 구속형 기하형태를 가지는데, 이는 그것이 또한 5원 고리를 갖기 때문이다.

본원에서 사용되는 바와 같이, "비극성 아미노산 또는 잔기"는 생리학적 pH에서 하전되어 있지 않고 2개 원자에 의해 공유된 한 쌍의 전자가 일반적으로 2개 원자 각각에 의해 동일하게 유지된 결합을 갖는 측쇄를 갖는(즉, 측쇄는 극성이 아님) 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 비극성 아미노산에는 L-Gly(G), L-Leu(L), L-Val(V), L-Ile(I), L-Met(M) 및 L-Ala(A)이 포함된다.

본원에서 사용되는 바와 같이, "지방족 아미노산 또는 잔기"는 지방족 탄화수소 측쇄를 갖는 소수성 아미노산 또는 잔기를 지칭한다. 유전자에 의해 코딩되는 지방족 아미노산에는 L-Ala(A), L-Val(V), L-Leu(L) 및 L-Ile(I)이 포함된다. 시스테인(또는 "L-Cys" 또는 "[C]")은 이것이 다른 L-Cys(C) 아미노산 또는 다른 술파닐- 또는 술프히드릴 함유 아미노산과 디술파이드 다리를 형성할 수 있다는 점에서 특이하다는 사실에 유의해야 한다. "시스테인 유사 잔기"는 시스테인, 및 디술파이드 다리의 형성에 이용할 수 있는 술프히드릴 모이어티를 함유하는 다른 아미노산을 포함한다. L-Cys(C)(및 -SH 함유 측쇄를 갖는 다른 아미노산)이 환원된 유리 -SH 또는 산화된 디술파이드 다리 형태의 펩타이드에 존재하는 능력은, L-Cys(C)가 펩타이드에 대한 전체적인 소수성 또는 친수성 특성에 기여하는지의 여부에 영향을 미친다. L-Cys(C)이 아이젠베르크(Eisenberg)의 표준화된 합의된 소수성 척도(Eisenberg et al., 1984, 상기 문헌)에 따라 0.29의 소수도를 나타내긴 하지만, 본 개시내용의 목적을 위해 L-Cys(C)은 그의 자체의 특유한 기로 분류됨을 이해하여야 한다.

본원에서 사용되는 바와 같이, "소형 아미노산 또는 잔기"는 총 3개 이하의 탄소 및/또는 헤테로원자(α-탄소 및 수소 제외)로 이루어지는 측쇄를 갖는 아미노산 또는 잔기를 지칭한다. 소형 아미노산 또는 잔기는 상기 정의에 따라 지방족, 비극성, 극성 또는 산성 소형 아미노산 또는 잔기로 추가로 분류될 수 있다. 유전자에 의해 코딩되는 소형 아미노산은 L-Ala(A), L-Val(V), L-Cys(C), L-Asn(N), L-Ser(S), L-Thr(T) 및 L-Asp(D)을 포함한다.

본원에서 사용되는 바와 같이, "하이드록실 함유 아미노산 또는 잔기"는 하이드록실(-OH) 모이어티를 함유하는 아미노산을 지칭한다. 유전자에 의해 코딩되는 하이드록실 함유 아미노산에는 L-Ser(S), L-Thr(T) 및 L-Tyr(Y)이 포함된다.

본원에서 사용되는 바와 같이, "폴리뉴클레오타이드" 및 "핵산"은 서로 공유 결합된 2개 이상의 뉴클레오타이드를 지칭한다. 폴리뉴클레오타이드는 전적으로 리보뉴클레오타이드(즉, RNA)로 이루어지거나, 전적으로 2' 데옥시리보뉴클레오타이드(즉, DNA)로 이루어지거나, 또는 리보- 및 2' 데옥시리보뉴클레오타이드의 혼합물로 이루어질 수 있다. 뉴클레오사이드는 전형적으로 표준 포스포디에스테르 결합을 통해 서로 연결될 것이지만, 폴리뉴클레오타이드는 하나 이상의 비표준 결합을 포함할 수 있다. 폴리뉴클레오타이드는 단일 가닥 또는 이중 가닥일 수 있거나, 또는 단일 가닥 영역 및 이중 가닥 영역 둘 모두를 포함할 수 있다. 또한, 폴리뉴클레오타이드는 전형적으로 자연 발생 코딩 핵염기(즉, 아데닌, 구아닌, 우라실, 티민 및 시토신)로 이루어질 것이지만, 그것은 하나 이상의 변형된 핵염기 및/또는 합성 핵염기, 예를 들어, 이노신, 잔틴, 히포잔틴 등을 포함할 수 있다. 일부 실시양태에서, 이러한 변형된 핵염기 또는 합성 핵염기는 아미노산 서열을 코딩하는 핵염기이다.

본원에서 사용되는 바와 같이, "뉴클레오사이드"는 핵염기(즉, 질소 염기) 및 5-탄소 당(예를 들어, 리보스 또는 데옥시리보스)을 포함하는 글리코실아민을 지칭한다. 뉴클레오사이드의 비제한적인 예는 시티딘, 우리딘, 아데노신, 구아노신, 티미딘 및 이노신을 포함한다. 이와 대조적으로, 용어 "뉴클레오타이드"는 핵염기, 5-탄소 당 및 하나 이상의 포스페이트 기를 포함하는 글리코실아민을 지칭한다. 일부 실시양태에서, 뉴클레오사이드는 뉴클레오타이드를 생성하기 위해 키나제에 의해 인산화될 수 있다.

본원에서 사용되는 바와 같이, "뉴클레오사이드 디포스페이트"는 핵염기(즉, 질소 염기), 5-탄소 당(예를 들어, 리보스 또는 데옥시리보스), 및 디포스페이트(즉, 피로포스페이트) 모이어티를 포함하는 글리코실아민을 지칭한다. 본원의 일부 실시양태에서, "뉴클레오사이드 디포스페이트"는 "NDP"로 약칭된다. 뉴클레오사이드 디포스페이트의 비제한적인 예는 시티딘 디포스페이트(CDP), 우리딘 디포스페이트(UDP), 아데노신 디포스페이트(ADP), 구아노신 디포스페이트(GDP), 티미딘 디포스페이트(TDP) 및 이노신 디포스페이트(IDP)를 포함한다. 용어 "뉴클레오사이드" 및 "뉴클레오타이드"는 일부 문맥에서 상호 교환 가능하게 사용될 수 있다.

본원에서 사용되는 바와 같이, "코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예를 들어, 유전자) 부분을 지칭한다.

본원에서 사용되는 바와 같이, 용어 "생촉매반응," "생촉매성," "생체변환," 및 "생합성"은 유기 화합물에 대한 화학 반응을 수행하는 효소의 사용을 지칭한다.

본원에서 사용되는 바와 같이, "야생형" 및 "자연 발생"은 자연계에서 발견되는 형태를 지칭한다. 예를 들어, 야생형 폴리펩타이드 또는 폴리뉴클레오타이드 서열은 자연계의 공급원으로부터 단리될 수 있으며 인간 조작에 의해 의도적으로 변형되지 않은 유기체 내에 존재하는 서열이다.

본원에서 사용되는 바와 같이, 세포, 핵산, 또는 폴리펩타이드와 함께 사용될 때 "재조합", "조작된" 및 "비자연 발생"은 자연계에 존재하지 않을 방식으로 변형된 물질, 또는 상기 물질의 자연 또는 천연 형태에 상응하는 물질을 지칭한다. 일부 실시양태에서, 세포, 핵산 또는 폴리펩타이드는 자연 발생 세포, 핵산 또는 폴리펩타이드와 동일하지만, 합성 물질로부터 및/또는 재조합 기술을 사용한 조작에 의해 생산되거나 유래된다. 비제한적인 예는 특히 세포의 천연(비-재조합)) 형태 내에서 발견되지 않는 유전자를 발현하는 재조합 세포 또는 상이한 수준으로 발현되는 천연 유전자를 발현하는 재조합 세포를 포함한다.

용어 "서열 동일성 비율(%)"은 폴리뉴클레오타이드 또는 폴리펩타이드 사이의 비교를 지칭하기 위해 본원에서 사용되며, 비교 윈도우에서 최적으로 정렬된 2개의 서열을 비교함으로써 결정되고, 여기서 비교 윈도우 내의 폴리뉴클레오타이드 또는 폴리펩타이드 서열의 부분은 2개의 서열의 최적 정렬을 위해 참조 서열과 비교하여 부가 또는 결실(즉, 갭)을 포함할 수 있다. 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열에 존재하는 위치의 수를 결정하여 매치된 위치의 수를 산출하는 단계, 매치된 위치의 수를 비교 윈도우 내의 위치의 총 수로 나누고 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출하는 단계에 의해 계산될 수 있다. 대안적으로, 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열에 존재하거나 핵산 염기 또는 아미노산 잔기가 갭과 정렬되는 위치의 수를 결정하여 매치된 위치의 수를 산출하는 단계, 매치된 위치의 수를 비교 윈도우 내의 위치의 총 수로 나누고 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출하는 단계에 의해 계산될 수 있다. 관련 기술 분야에서 통상의 기술을 가진 자는 2개의 서열을 정렬하는데 이용가능한 많은 확립된 알고리즘이 있다는 것을 이해한다. 비교를 위한 서열의 최적 정렬은, 관련 기술 분야에 공지된 바와 같이, 스미스(Smith) 및 워터만(Waterman)의 국소 상동성 알고리즘(Smith and Waterman, Adv. Appl. Math., 2:482 [1981]), 니들만(Needleman) 및 분쉬(Wunsch)의 상동성 정렬 알고리즘(Needleman and Wunsch, J. Mol., Biol., 48:443 [1970]), 피어슨(Pearson) 및 립만(Lipman)의 유사성 검색 방법(Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]), 이들 알고리즘의 컴퓨터에 의한 실행(예를 들어, GCG 위스콘신 소프트웨어 패키지 내의 GAP, BESTFIT, FASTA, 및 TFASTA), 또는 육안 검사를 포함하고 이로 제한되지 않는 임의의 적합한 방법에 의해 수행될 수 있다. 서열 동일성 및 서열 유사성 비율을 결정하는데 적합한 알고리즘의 예는 BLAST 및 BLAST 20 알고리즘을 포함하지만 이로 제한되지 않으며, 이는 알츌(Altschul) 등에 의해 설명되어 있다(각각, 문헌 [Altschul et al., J. Mol. Biol., 215: 403-410 [1990]; 및 [Altschul et al., Nucl. Acids Res., 3389-3402 [1977] 참조). BLAST 분석을 수행하기 위한 소프트웨어는 미국 국립 생물 정보 센터 웹사이트를 통해 공개적으로 이용가능하다. 이 알고리즘은 데이터베이스 서열 내의 동일한 길이의 워드와 정렬될 때 일부 양의 값의 역치 스코어 T와 일치하거나 이를 충족하는, 질의 서열 내의 길이 W의 짧은 워드를 확인함으로써 높은 스코어링 서열 쌍(HSP)을 먼저 확인하는 것을 포함한다. T는 이웃 워드 스코어 역치로도 불린다(상기 문헌 [Altschul et al.] 참조). 이들 초기 이웃 워드 히트(hit)는 이들을 함유하는 더 긴 HSP를 찾기 위한 검색을 개시하는 씨드(seed)로서 작용한다. 이후, 워드 히트는 누적 정렬 스코어가 증가될 수 있을 때까지 각각의 서열을 따라 양 방향으로 연장된다. 누적 스코어는 뉴클레오타이드 서열에 대해, 파라미터 M(매칭 잔기 쌍에 대한 보상 스코어; 항상 >0) 및 N(미스매칭 잔기에 대한 페널티 스코어; 항상 <0)을 사용하여 계산된다. 아미노산 서열의 경우, 누적 스코어를 계산하기 위해 스코어링 매트릭스(scoring matrix)가 사용된다. 각각의 방향에서 워드 히트의 연장은 누적 정렬 스코어가 그의 최대 달성 값으로부터 양 X만큼 떨어질 때; 누적 스코어가 하나 이상의 음성 스코어링 잔기 정렬의 누적으로 인해 0 이하가 될 때; 또는 하나의 서열의 말단에 도달할 때 중지된다. BLAST 알고리즘 파라미터 W, T, 및 X는 정렬의 민감성 및 속도를 결정한다. BLASTN 프로그램(뉴클레오타이드 서열용)은 디폴트로서 워드 길이(W) 11, 기대값(E) 10, M=5, N=-4, 및 두 가닥의 비교를 사용한다. 아미노산 서열의 경우, BLASTP 프로그램은 디폴트로서 워드 길이(W) 3, 기대값(E) 10, 및 BLOSUM62 스코어링 매트릭스를 사용한다(문헌 [Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]] 참조). 서열 정렬 및 서열 동일성 %의 예시적인 결정은 제공된 디폴트 파라미터를 사용하여, GCG 위스콘신 소프트웨어 패키지(Accelrys, 미국 위스콘신주 매디슨 소재) 내의 BESTFIT 또는 GAP 프로그램을 사용할 수 있다.

"참조 서열"은 서열 및/또는 활성 비교를 위한 기준으로서 사용되는 정의된 서열을 지칭한다. 참조 서열은 더 큰 서열의 서브세트, 예를 들어, 전장 유전자 또는 폴리펩타이드 서열의 세그먼트일 수 있다. 일반적으로, 참조 서열은 핵산 또는 폴리펩타이드의 적어도 20개 뉴클레오타이드 또는 아미노산 잔기 길이, 적어도 25개 잔기 길이, 적어도 50개 잔기 길이, 적어도 100개 잔기 길이 또는 전장이다. 2개의 폴리뉴클레오타이드 또는 폴리펩타이드는 각각 (1) 2개의 서열 사이에 유사한 서열(즉, 완전한 서열의 일부)을 포함할 수 있고, 및 (2) 2개의 서열 사이에 상이한 서열을 추가로 포함할 수 있으므로, 2개(또는 그 초과)의 폴리뉴클레오타이드 또는 폴리펩타이드 사이의 서열 비교는 전형적으로 "비교 윈도우"에서 2개의 폴리뉴클레오타이드 또는 폴리펩타이드의 서열을 비교하여 서열 유사성의 국소 영역을 확인하고 비교함으로써 수행된다. 일부 실시양태에서, "참조 서열"은 일차 아미노산 서열에 기초할 수 있으며, 여기서 참조 서열은 일차 서열에서 하나 이상의 변화를 가질 수 있는 서열이다.

본원에서 사용되는 바와 같이, "비교 윈도우"는 적어도 약 20개의 인접한 뉴클레오타이드 위치 또는 아미노산 잔기의 개념적 세그먼트를 지칭하며, 여기서 서열은 적어도 20개의 인접한 뉴클레오타이드 또는 아미노산의 참조 서열과 비교될 수 있고, 여기서 비교 윈도우 내의 서열의 일부는 2개의 서열의 최적 정렬을 위한 참조 서열(부가 또는 결실을 포함하지 않음)과 비교하여 20% 이하의 부가 또는 결실(즉, 갭)을 포함할 수 있다. 비교 윈도우는 20개 초과의 인접한 잔기일 수 있고, 선택적으로 30, 40, 50, 100개 또는 그 초과의 윈도우를 포함한다.

본원에서 사용되는 바와 같이, 주어진 아미노산 또는 폴리뉴클레오타이드 서열의 넘버링의 측면에서 사용될 때, "에 상응하는", "에 대한" 및 "와 비교하여"는 주어진 아미노산 또는 폴리뉴클레오타이드 서열이 참조 서열과 비교될 때 특정 참조 서열의 잔기의 넘버링을 지칭한다. 즉, 주어진 중합체의 잔기 번호 또는 잔기 위치는 주어진 아미노산 또는 폴리뉴클레오타이드 서열 내의 잔기의 실제 수치적 위치에 의해서가 아니라 참조 서열에 대해 지정된다. 예를 들어, 조작된 퓨린 뉴클레오사이드 포스포릴라제의 아미노산 서열과 같은 주어진 아미노산 서열은 2개의 서열 사이의 잔기 매치를 최적화하기 위해 갭을 도입함으로써 참조 서열에 정렬될 수 있다. 이 경우, 비록 갭이 존재하지만, 주어진 아미노산 또는 폴리뉴클레오타이드 서열에서의 잔기의 넘버링은 그것이 정렬된 참조 서열에 대해 이루어진다.

본원에서 사용되는 바와 같이, "실질적인 동일성"은 적어도 20개 잔기 위치의 비교 윈도우에서, 흔히 적어도 30-50개 잔기의 윈도우에서 참조 서열과 비교하여 적어도 80 퍼센트의 서열 동일성, 적어도 85 퍼센트의 동일성, 적어도 89 내지 95 퍼센트의 서열 동일성, 또는 보다 일반적으로, 적어도 99 퍼센트의 서열 동일성을 갖는 폴리뉴클레오타이드 또는 폴리펩타이드 서열을 지칭하며, 여기서 서열 동일성의 백분율은 비교 윈도우에서 참조 서열을 참조 서열의 총 20 퍼센트 이하의 결실 또는 부가를 포함하는 서열과 비교함으로써 계산된다. 폴리펩타이드에 적용된 일부 특정 실시양태에서, 용어 "실질적인 동일성"은 2개의 폴리펩타이드 서열이 예를 들어 디폴트 갭 가중치를 사용하여 프로그램 GAP 또는 BESTFIT에 의해 최적으로 정렬될 때, 적어도 80 퍼센트의 서열 동일성, 바람직하게는 적어도 89 퍼센트의 서열 동일성, 적어도 95 퍼센트의 서열 동일성 또는 그 초과의 서열 동일성(예를 들어, 99 퍼센트의 서열 동일성)을 공유하는 것을 의미한다. 일부 실시양태에서, 비교되는 서열에서 동일하지 않은 잔기 위치는 보존적 아미노산 치환에 의해 달라진다.

본원에서 사용되는 바와 같이, "아미노산 차이" 및 "잔기 차이"는 참조 서열 내의 상응하는 위치에 있는 아미노산 잔기와 비교하여 폴리펩타이드 서열의 위치에서의 아미노산 잔기의 차이를 지칭한다. 일부 경우에, 참조 서열에는 히스티딘 태그가 있지만, 넘버링은 히스티딘 태그가 없는 동등한 참조 서열에서와 같이 유지된다. 아미노산 차이의 위치는 일반적으로 본원에서 "Xn"으로 지칭되며, 여기서 n은 잔기 차이가 기초로 하는 참조 서열 내의 상응하는 위치를 지칭한다. 예를 들어, "서열 번호 4와 비교하여 위치 X93에서의 잔기 차이"는 서열 번호 4의 위치 93에 상응하는 폴리펩타이드 위치에서의 아미노산 잔기의 차이를 지칭한다. 따라서, 서열 번호 4의 참고 폴리펩타이드가 위치 93에 세린을 가지면, "서열 번호 4와 비교하여 위치 X93에서의 잔기 차이"는 서열 번호 4의 위치 93에 상응하는 폴리펩타이드의 위치에서의 세린 이외의 임의의 다른 잔기의 아미노산 치환을 지칭한다. 본원에서 대부분의 경우, 위치에서의 특정 아미노산 잔기 차이는 "XnY"로 표시되며, 여기서 "Xn"은 전술한 바와 같이 상응하는 위치를 특정하고, "Y"는 조작된 폴리펩타이드에서 발견되는 아미노산(즉, 참고 폴리펩타이드와 상이한 잔기)의 단일 글자 식별자이다. 일부 경우에(예를 들어, 실시예에서 제시되는 표에서), 본 발명은 또한 종래의 표기법 "AnB"에 의해 표시된 특정 아미노산 차이를 제공하며, 여기서 A는 참조 서열에서의 잔기의 단일 글자 식별자이고, "n"은 참조 서열에서의 잔기 위치의 번호이며, B는 조작된 폴리펩타이드의 서열에서의 잔기 치환의 단일 글자 식별자이다. 일부 경우에, 본 발명의 폴리펩타이드는 참조 서열과 비교하여 하나 이상의 아미노산 잔기 차이를 포함할 수 있고, 이는 참조 서열과 비교하여 잔기 차이가 존재하는 특정 위치의 목록에 의해 표시된다. 일부 실시양태에서, 둘 이상의 아미노산이 폴리펩타이드의 특정 잔기 위치에서 사용될 수 있는 경우, 사용될 수 있는 다양한 아미노산 잔기는 "/"에 의해 분리된다(예를 들어, X307H/X307P 또는 X307H/P). 슬래시는 또한 주어진 변이체 내의 다수의 치환을 나타내기 위해 사용될 수 있다(즉, 조합 변이체에서와 같이, 주어진 서열에 둘 이상의 치환이 존재함). 일부 실시양태에서, 본 발명은 보존적 또는 비보존적 아미노산 치환을 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩타이드 서열을 포함한다. 일부 추가의 실시양태에서, 본 발명은 보존적 및 비보존적 아미노산 치환을 모두 포함하는 조작된 폴리펩타이드 서열을 제공한다.

본원에서 사용되는 바와 같이, "보존적 아미노산 치환"은 잔기를 유사한 측쇄를 갖는 상이한 잔기로 치환하는 것을 지칭하며, 따라서 전형적으로 폴리펩타이드 내의 아미노산을 동일하거나 유사한 정의된 아미노산 클래스 내의 아미노산으로 치환하는 것을 포함한다. 비제한적인 예를 들면, 일부 실시양태에서, 지방족 측쇄를 갖는 아미노산은 또 다른 지방족 아미노산(예를 들어, 알라닌, 발린, 류신, 및 이소류신)으로 치환되고/되거나; 하이드록실 측쇄를 갖는 아미노산은 하이드록실 측쇄를 갖는 또 다른 아미노산(예를 들어, 세린 및 트레오닌)으로 치환되고/되거나; 방향족 측쇄를 갖는 아미노산은 방향족 측쇄를 갖는 또 다른 아미노산(예를 들어, 페닐알라닌, 티로신, 트립토판, 및 히스티딘)으로 치환되고/되거나; 염기성 측쇄를 갖는 아미노산은 염기성 측쇄를 갖는 또 다른 아미노산(예를 들어, 리신 및 아르기닌)으로 치환되고/되거나; 산성 측쇄를 갖는 아미노산은 산성 측쇄를 갖는 또 다른 아미노산(예를 들어, 아스파르트산 또는 글루탐산)으로 치환되고/되거나; 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다.

본원에서 사용되는 바와 같이, "비보존적 치환"은 폴리펩타이드 내의 아미노산을 유의하게 상이한 측쇄 특성을 갖는 아미노산으로 치환하는 것을 지칭한다. 비보존적 치환은 정의된 기 내에서보다는 정의된 기 사이에서 아미노산을 사용할 수 있고, (a) 치환 영역 내의 펩타이드 백본의 구조(예를 들어, 글리신을 프롤린으로), (b) 전하 또는 소수성, 또는 (c) 측쇄의 부피에 영향을 미친다. 비제한적인 예를 들어, 예시적인 비보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산; 작은 아미노산으로 치환된 방향족 아미노산; 및 소수성 아미노산으로 치환된 친수성 아미노산일 수 있다.

본원에서 사용되는 바와 같이, "결실"은 참고 폴리펩타이드로부터 하나 이상의 아미노산의 제거에 의한 폴리펩타이드의 변형을 지칭한다. 결실은 조작된 퓨린 뉴클레오사이드 포스포릴라제 효소의 효소 활성을 유지하고/하거나 개선된 특성을 유지하면서 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산, 또는 20개 이상의 아미노산, 참고 효소를 구성하는 아미노산의 총 수의 최대 10%, 또는 아미노산의 총 수의 최대 20%의 제거를 포함할 수 있다. 결실은 폴리펩타이드의 내부 부위 및/또는 말단 부위에 관한 것일 수 있다. 다양한 실시양태에서, 결실은 연속적인 세그먼트을 포함할 수 있거나 불연속적일 수 있다. 결실은 일반적으로 아미노산 서열에 "-"로 표시된다.

본원에서 사용되는 바와 같이, "삽입"은 참고 폴리펩타이드로부터 하나 이상의 아미노산의 부가에 의한 폴리펩타이드에 대한 변형을 지칭한다. 삽입은 폴리펩타이드의 내부 부분, 또는 카르복시 또는 아미노 말단에 있을 수 있다. 본원에서 사용되는 바와 같이 삽입은 관련 기술 분야에 공지된 바와 같은 융합 단백질을 포함한다. 삽입은 아미노산의 인접한 세그먼트일 수 있거나 또는 자연 발생 폴리펩타이드 내의 아미노산 중 하나 이상에 의해 분리될 수 있다.

용어 "아미노산 치환 세트" 또는 "치환 세트"는 참조 서열과 비교하여 폴리펩타이드 서열 내의 아미노산 치환의 군을 지칭한다. 치환 세트는 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15개 또는 그 초과의 아미노산 치환을 가질 수 있다. 일부 실시양태에서, 치환 세트는 실시예에서 제공되는 표에 나열된 임의의 변이체 퓨린 뉴클레오사이드 포스포릴라제에 존재하는 아미노산 치환 세트를 지칭한다.

"기능적 단편" 및 "생물학적 활성 단편"은 아미노 말단 및/또는 카르복시 말단 결실(들) 및/또는 내부 결실을 갖지만, 나머지 아미노산 서열은 비교되는 서열(예를 들어, 본 발명의 조작된 전장 퓨린 뉴클레오사이드 포스포릴라제) 내의 상응하는 위치와 동일하고 전장 폴리펩타이드의 활성을 실질적으로 모두 보유하는 폴리펩타이드를 지칭하기 위해 본원에서 상호 교환 가능하게 사용된다.

본원에서 사용되는 바와 같이, "단리된 폴리펩타이드"는 자연적으로 동반되는 다른 오염물질(예를 들어, 단백질, 지질, 및 폴리뉴클레오타이드)로부터 실질적으로 분리된 폴리펩타이드를 지칭한다. 이 용어는 이들의 자연 발생 환경 또는 발현 시스템(예를 들어, 숙주 세포 내에서 또는 시험관내 합성을 통해)으로부터 제거되거나 정제된 폴리펩타이드를 포함한다. 재조합 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드는 세포 내에 존재하거나, 세포 배지에 존재하거나, 또는 용해물 또는 단리된 제제와 같은 다양한 형태로 제조될 수 있다. 따라서, 일부 실시양태에서, 재조합 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드는 단리된 폴리펩타이드일 수 있다.

본원에서 사용되는 바와 같이, "실질적으로 순수한 폴리펩타이드"는 폴리펩타이드 종이 존재하는 우세한 종(즉, 몰 또는 중량 기준으로, 조성물 내의 임의의 다른 개별적인 거대분자 종보다 더 풍부함)인 조성물을 지칭하며, 일반적으로 대상 종이 존재하는 거대분자 종의 몰 또는 중량% 기준으로 적어도 약 50 퍼센트를 포함할 때 실질적으로 정제된 조성물이다. 그러나, 일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제를 포함하는 조성물은 50% 미만으로 순수한(예를 들어, 약 10%, 약 20%, 약 30%, 약 40%, 또는 약 50%) 퓨린 뉴클레오사이드 포스포릴라제를 포함한다. 일반적으로, 실질적으로 순수한 퓨린 뉴클레오사이드 포스포릴라제 조성물은 조성물에 존재하는 모든 거대분자 종의 몰 또는 중량% 기준으로 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 95% 이상, 및 약 98% 또는 그 초과를 포함한다. 일부 실시양태에서, 대상 종은 본질적인 균질성(즉, 오염 종은 종래의 검출 방법에 의해 조성물에서 검출될 수 없음)까지 정제되며, 여기서 조성물은 본질적으로 단일 거대분자 종으로 이루어진다. 용매 종, 작은 분자(<500 달톤), 및 원소 이온 종은 거대분자 종으로 간주되지 않는다. 일부 실시양태에서, 단리된 재조합 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드는 실질적으로 순수한 폴리펩타이드 조성물이다.

본원에서 사용되는 바와 같이, "개선된 효소 특성"은 효소의 적어도 하나의 개선된 특성을 지칭한다. 일부 실시양태에서, 본 발명은 참조 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 및/또는 야생형 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드, 및/또는 또 다른 조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드와 비교하여 임의의 효소 특성의 개선을 나타내는 조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 제공한다. 따라서, "개선"의 수준은 야생형뿐만 아니라 조작된 퓨린 뉴클레오사이드 포스포릴라제를 포함하는, 다양한 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 사이에서 결정되고 비교될 수 있다. 개선된 특성은 단백질 발현 증가, 열활성 증가, 열안정성 증가, pH 활성 증가, 안정성 증가, 효소 활성 증가, 기질 특이성 또는 친화성 증가, 특이적 활성 증가, 기질 또는 최종 생성물 억제에 대한 내성 증가, 화학적 안정성 증가, 화학적 선택성 개선, 용매 안정성 개선, 산성 pH에 대한 내성 증가, 단백질 분해 활성에 대한 내성 증가(즉, 단백질 분해에 대한 민감성 감소), 응집 감소, 용해도 증가, 및 온도 프로파일 변경과 같은 특성을 포함하지만, 이로 제한되지 않는다. 추가의 실시양태에서, 상기 용어는 퓨린 뉴클레오사이드 포스포릴라제 효소의 적어도 하나의 개선된 특성과 관련하여 사용된다. 일부 실시양태에서, 본 발명은 참조 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 및/또는 야생형 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 및/또는 다른 조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드와 비교하여 임의의 효소 특성에서 개선을 나타내는 조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 제공한다. 따라서, "개선" 수준은 야생형을 포함한 다양한 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 및 조작된 퓨린 뉴클레오사이드 포스포릴라제 사이에서 결정되고 비교할 수 있다.

본원에서 사용되는 바와 같이, "증가된 효소적 활성" 및 "향상된 촉매 활성"은 조작된 폴리펩타이드의 개선된 특성을 지칭하며, 이는 참조 효소와 비교하여 특이적 활성(예를 들어, 생성된 생성물/시간/중량 단백질)의 증가 또는 기질로부터 생성물로의 전환 비율(예를 들어, 효소의 특정 양을 사용하여 특정 시간 내에 기질의 출발 양에서 생성물로의 전환 비율)의 증가에 의해 표시될 수 있다. 일부 실시양태에서, 상기 용어는 본원에서 제공되는 조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 개선된 특성을 지칭하며, 이는 참조 퓨린 뉴클레오사이드 포스포릴라제 효소와 비교하여 특이적 활성(예를 들어, 생성된 생성물/시간/중량 단백질)의 증가 또는 기질로부터 생성물로의 전환 비율(예를 들어, 퓨린 뉴클레오사이드 포스포릴라제의 특정 양을 사용하여 특정 시간 내에 기질의 출발 양에서 생성물로의 전환 비율)의 증가에 의해 표시될 수 있다. 일부 실시양태에서, 상기 용어는 본원에서 제공되는 개선된 퓨린 뉴클레오사이드 포스포릴라제 효소와 관련하여 사용된다. 본 발명의 조작된 퓨린 뉴클레오사이드 포스포릴라제의 효소 활성을 결정하는 예시적인 방법이 실시예에 제공되어 있다. K _m , V _max 또는 k _cat 의 고전적인 효소 특성을 포함하는 효소 활성과 관련된 임의의 특성은 영향을 받을 수 있고, 이들의 변화는 효소적 활성을 증가시킬 수 있다. 예를 들어, 효소 활성의 개선은 상응하는 야생형 효소의 효소 활성의 약 1.1배로부터, 자연 발생 퓨린 뉴클레오사이드 포스포릴라제 또는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드가 그로부터 유래되는 또 다른 조작된 퓨린 뉴클레오사이드 포스포릴라제보다 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배, 150배, 200배 또는 그 초과의 효소 활성만큼 클 수 있다.

본원에서 사용되는 바와 같이, "전환"은 기질(들)에서 상응하는 생성물(들)로의 효소에 의한 전환(또는 생체변환)을 지칭한다. "전환 비율"은 특정 조건 하에 일정 기간 내에 생성물로 전환되는 기질의 비율을 지칭한다. 따라서, 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 "효소 활성" 또는 "활성"은 특정 시간 내에 기질의 생성물로의 "전환 비율"로 표현될 수 있다.

"전반적인(generalist) 특성"을 갖는 효소(또는 "전반적인(generalist) 효소")는 모 서열과 비교하여 광범위한 기질에 대해 개선된 활성을 나타내는 효소를 지칭한다. 전반적인 효소는 반드시 모든 가능한 기질에 대해 개선된 활성을 나타내는 것은 아니다. 일부 실시양태에서, 본 발명은 광범위한 입체적으로 및 전자적으로 다양한 기질에 대해서 모 유전자와 비교하여 유사하거나 개선된 활성을 나타낸다는 점에서 전반적인 특성을 갖는 퓨린 뉴클레오사이드 포스포릴라제 변이체를 제공한다. 또한, 본원에서 제공되는 전반적인 효소는 대사산물/생성물의 생산을 증가시키기 위해 광범위한 다양한 분자에 대해 개선되도록 조작되었다.

용어 "엄격한 혼성화 조건"은 핵산 혼성체가 안정한 조건을 지칭하기 위해 본원에서 사용된다. 관련 기술 분야에서 통상의 기술을 가진 자에게 공지된 바와 같이, 혼성체의 안정성은 혼성체의 융점(T _m )에 반영된다. 일반적으로, 혼성체의 안정성은 이온 강도, 온도, G/C 함량, 및 카오트로픽제(chaotropic agent)의 존재의 함수이다. 폴리뉴클레오타이드의 T _m 값은 융점을 예측하는 공지된 방법을 사용하여 계산될 수 있다(예를 들어, 문헌 [Baldino et al., Meth. Enzymol., 168:761-777 [1989]; Bolton et al., Proc. Natl. Acad. Sci. USA 48:1390 [1962]; Bresslauer et al., Proc. Natl. Acad. Sci. USA 83:8893-8897 [1986]; Freier et al., Proc. Natl. Acad. Sci. USA 83:9373-9377 [1986]; Kierzek et al., Biochem., 25:7840-7846 [1986]; Rychlik et al., Nucl. Acids Res., 18:6409-6412 [1990](erratum, Nucl. Acids Res., 19:698 [1991]); Sambrook et al., supra); Suggs et al., 1981, in Developmental Biology Using Purified Genes, Brown et al. [eds.], pp. 683-693, Academic Press, Cambridge, MA [1981]; 및 Wetmur, Crit. Rev. Biochem. Mol. Biol. 26:227-259 [1991] 참조). 일부 실시양태에서, 폴리뉴클레오타이드는 본원에서 개시되는 폴리펩타이드를 코딩하며, 중간 정도로 엄격한 또는 매우 엄격한 조건과 같은 정의된 조건 하에 본 발명의 조작된 퓨린 뉴클레오사이드 포스포릴라제 효소를 코딩하는 서열의 보체에 혼성화한다.

본원에서 사용되는 바와 같이, "혼성화 엄격성"은 핵산의 혼성화에서 세척 조건과 같은 혼성화 조건에 관한 것이다. 일반적으로, 혼성화 반응은 더 낮은 엄격성의 조건 하에서 수행된 다음, 다양하지만 더 높은 엄격성의 세척이 수행된다. 용어 "중간 정도의 엄격한 혼성화"는 표적-DNA가 표적 DNA에 대해 약 60%의 동일성, 바람직하게는 약 75%의 동일성, 약 85%의 동일성, 표적-폴리뉴클레오타이드에 대해 약 90% 초과의 동일성을 갖는 상보적인 핵산에 결합하게 하는 조건을 지칭한다. 예시적인 중간 정도의 엄격한 조건은 42℃에서 50% 포름아미드, 5x 덴하르트 용액, 5x SSPE, 0.2% SDS에서의 혼성화 후, 42℃에서 0.2xSSPE, 0.2% SDS에서의 세척과 동등한 조건이다. "높은 엄격성 혼성화"는 일반적으로 정의된 폴리뉴클레오타이드 서열에 대해 용액 조건 하에서 결정된 바와 같은 열 융점 T _m 으로부터 약 10℃ 이하인 조건을 지칭한다. 일부 실시양태에서, 높은 엄격성 조건은 65℃에서 0.018 M NaCl에서 안정한 혼성체를 형성하는 핵산 서열만의 혼성화를 허용하는 조건을 지칭한다(즉, 혼성체가 65℃에서 0.018 M NaCl에서 안정하지 않으면, 이것은 본원에서 고려되는 바와 같은 높은 엄격성 조건 하에서 안정하지 않을 것임). 높은 엄격성 조건은 예를 들어 42℃에서 50% 포름아미드, 5x 덴하르트 용액, 5x SSPE, 0.2% SDS와 동등한 조건에서의 혼성화 후, 65℃에서 0.1x SSPE, 및 0.1% SDS에서의 세척에 의해 제공될 수 있다. 또 다른 높은 엄격성 조건은 65℃에서 0.1%(w/v) SDS를 함유하는 5x SSC에서의 혼성화와 동등한 조건에서의 혼성화 및 65℃에서 0.1% SDS를 함유하는 0.1x SSC에서의 세척이다. 다른 높은 엄격성 혼성화 조건뿐만 아니라 중간 정도의 엄격한 조건은 상기 인용된 참고문헌에 기재되어 있다.

본원에서 사용되는 바와 같이, "코돈 최적화된"은 단백질을 코딩하는 폴리뉴클레오타이드의 코돈이 코딩된 단백질이 관심있는 유기체에서 효율적으로 발현되도록 특정 유기체에서 우선적으로 사용되는 것으로 변화하는 것을 지칭한다. 유전자 코드는 대부분의 아미노산이 "동의어" 또는 "동의" 코돈으로 불리는 몇 개의 코돈에 의해 표시된다는 점에서 축퇴성이지만, 특정 유기체에 의한 코돈 사용 빈도는 무작위적이 아니며, 특정 코돈 삼중자(triplet)로 편향된다는 것이 널리 알려져 있다. 이 코돈 사용 빈도 편향은 주어진 유전자, 공통 기능 또는 조상 기원의 유전자, 고도로 발현된 단백질 대 낮은 카피수 단백질, 및 유기체 게놈의 전체적인 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 효소를 코딩하는 폴리뉴클레오타이드는 발현을 위해 선택된 숙주 유기체에서 최적 생산을 위해 코돈 최적화될 수 있다.

본원에서 사용되는 바와 같이, "바람직한", "최적" 및 "높은 코돈 사용 빈도 편향" 코돈은 단독으로 또는 조합하여 사용될 때 동일한 아미노산을 코딩하는 다른 코돈보다 단백질 코딩 영역에서 더 높은 빈도로 사용되는 코돈을 상호 교환 가능하게 지칭한다. 바람직한 코돈은 단일 유전자, 공통 기능 또는 기원의 유전자 세트, 고도로 발현된 유전자에서의 코돈 사용 빈도, 전체 유기체의 전체적인 단백질 코딩 영역에서의 코돈 빈도, 관련 유기체의 전체적인 단백질 코딩 영역에서의 코돈 빈도, 또는 이들의 조합과 관련하여 결정될 수 있다. 유전자 발현 수준에 따라 그 빈도가 증가하는 코돈은 전형적으로 발현을 위한 최적 코돈이다. 특정 유기체에서 코돈 빈도(예를 들어, 코돈 사용 빈도, 상대적인 동의 코돈 사용 빈도) 및 코돈 선호도를 결정하기 위한 다양한 방법이 알려져 있으며, 이는 예를 들어 클러스터 분석 또는 대응 분석(correspondence analysis), 및 유전자에 사용된 코돈의 유효 수를 사용하는 다변량 분석을 포함한다(예를 들어, GCG CodonPreference, Genetics Computer Group Wisconsin Package; CodonW, Peden, University of Nottingham; McInerney, Bioinform., 14:372-73 [1998]; Stenico et al., Nucl. Acids Res., 222437-46 [1994]; 및 Wright, Gene 87:23-29 [1990] 참조). 많은 상이한 유기체에 대해 코돈 사용 빈도 표가 이용 가능하다(예를 들어, Wada et al., Nucl. Acids Res., 20:2111-2118 [1992]; Nakamura et al., Nucl. Acids Res., 28:292 [2000]; Duret, et al., supra; Henaut and Danchin, in Escherichia coli and Salmonella , Neidhardt, et al. (eds.), ASM Press, Washington D.C., p. 2047-2066 [1996] 참조). 코돈 사용 빈도를 얻기 위한 데이터 소스는 단백질을 코딩할 수 있는 임의의 이용 가능한 뉴클레오타이드 서열에 의존할 수 있다. 이들 데이터 세트는 발현된 단백질(예를 들어, 완전한 단백질 코딩 서열-CDS), 발현된 서열 태그(ESTS), 또는 게놈 서열의 예측된 코딩 영역을 코딩하는 것으로 실제 알려진 핵산 서열을 포함한다(예를 들어, Mount, Bioinformatics: Sequence and Genome Analysis, Chapter 8, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. [ 2001]; Uberbacher, Meth. Enzymol., 266:259-281 [1996]; 및 Tiwari et al., Comput. Appl. Biosci., 13:263-270 [1997] 참조).

본원에서 사용되는 바와 같이, "제어 서열"은 본 발명의 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현에 필요하거나 유리한 모든 성분을 포함한다. 각각의 제어 서열은 폴리펩타이드를 코딩하는 핵산 서열에 대해 천연 서열이거나 외래 서열일 수 있다. 이러한 제어 서열은 리더, 폴리아데닐화 서열, 프로펩타이드 서열, 프로모터 서열, 신호 펩타이드 서열, 개시 서열 및 전사 종결자를 포함하지만, 이로 제한되지 않는다. 최소한, 제어 서열은 프로모터, 및 전사 및 번역 정지 신호를 포함한다. 제어 서열은 폴리펩타이드를 코딩하는 핵산 서열의 코딩 영역과 제어 서열의 라이게이션을 용이하게 하는 특정 제한 부위를 도입하기 위한 목적으로 링커와 함께 제공될 수 있다.

"작동가능하게 연결된"은 제어 서열이 관심있는 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 유도하거나 조절하도록 제어 서열이 관심있는 폴리뉴클레오타이드와 관련된 위치에 적절하게 위치되는(즉, 기능적 관계로) 배열로서 본원에서 정의된다.

"프로모터 서열"은 코딩 서열과 같은 관심있는 폴리뉴클레오타이드의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 프로모터 서열은 관심있는 폴리뉴클레오타이드의 발현을 매개하는 전사 제어 서열을 함유한다. 프로모터는 돌연변이체 프로모터, 말단 절단된 프로모터, 및 혼성체 프로모터를 포함하는, 선택된 숙주 세포에서 전사 활성을 나타내는 임의의 핵산 서열일 수 있고, 이는 숙주 세포에 대해 동종 또는 이종인 세포외 또는 세포내 폴리펩타이드를 코딩하는 유전자로부터 수득될 수 있다.

문구 "적합한 반응 조건"은 본 발명의 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드가 기질을 원하는 생성물 화합물로 전환할 수 있는 효소 전환 반응 용액의 조건(예를 들어, 효소 로딩, 기질 로딩, 온도, pH, 완충제, 공용매 등의 범위)을 지칭한다. 일부 예시적인 "적합한 반응 조건"이 본원에서 제공된다.

본원에서 사용되는 바와 같이, "화합물 로딩" 또는 "효소 로딩"에서와 같은 "로딩"은 반응 시작시 반응 혼합물 내의 성분의 농도 또는 양을 지칭한다.

본원에서 사용되는 바와 같이, "효소에 의한 전환 반응 과정의 맥락에서 "기질"은 본원에서 제공되는 조작된 효소가 그에 대해 작용하는 화합물 또는 분자를 지칭한다.

본원에서 사용되는 바와 같이, 반응으로부터 생성물(예를 들어, 데옥시리보스 포스페이트 유사체)의 수율을 "증가시키는 것"은 동일한 기질 및 다른 치환체를 갖지만 관심있는 성분이 존재하지 않는 동일한 조건 하에서 수행된 반응과 비교하여, 반응 동안 존재하는 특정 성분(예를 들어, 퓨린 뉴클레오사이드 포스포릴라제 효소)이 더 많은 생성물이 생산되게 할 때 발생한다.

특정 효소의 양이 반응을 촉매하는데 참여하는 다른 효소와 비교하여 약 2%, 약 1%, 또는 약 0.1%(wt/wt) 미만이면, 반응은 상기 특정 효소가 "실질적으로 없다"고 언급된다.

본원에서 사용되는 바와 같이, 액체(예를 들어, 배양 브로쓰)를 "분획화하는 것"은 분리 과정(예를 들어, 염 침전, 컬럼 크로마토그래피, 크기 배제, 및 여과) 또는 이러한 과정의 조합을 적용하여 원하는 단백질이 초기 액체 생성물보다 용액에서 더 큰 백분율의 총 단백질을 포함하는 용액을 제공하는 것을 의미한다.

본원에서 사용되는 바와 같이, "출발 조성물"은 적어도 하나의 기질을 포함하는 임의의 조성물을 지칭한다. 일부 실시양태에서, 출발 조성물은 임의의 기질을 포함한다.

본원에서 사용되는 바와 같이, 효소 전환 과정과 관련하여 "생성물"은 기질에 대한 효소 폴리펩타이드의 작용으로부터 생성되는 화합물 또는 분자를 의미한다.

본원에서 사용되는 바와 같이, "평형화"는 화학 반응 또는 효소에 의한 반응(예를 들어, 두 종 A 및 B의 상호전환)에서 화학물질 종의 정상 상태 농도를 초래하는 과정을 의미하며, 여기에는 화학 반응 또는 효소에 의한 반응의 순방향 속도 상수 및 역방향 속도 상수에 의해 결정되는 바와 같은 입체 이성질체의 상호전환이 포함된다.

본원에서 사용되는 바와 같이, "알킬"은 직쇄 또는 분지쇄의 1 내지 18개의 탄소 원자, 보다 바람직하게는 1 내지 8개의 탄소 원자, 가장 바람직하게는 1 내지 6개의 탄소 원자의 포화 탄화수소기를 지칭한다. 특정 수의 탄소 원자를 갖는 알킬은 괄호 안에 표시된다(예를 들어, (C1-C4)알킬은 1 내지 4개의 탄소 원자의 알킬을 의미함).

본원에서 사용되는 바와 같이, "알케닐"은 적어도 하나의 이중 결합을 함유하지만 선택적으로 하나 초과의 이중 결합을 함유하는 직쇄 또는 분지쇄의 2 내지 12개의 탄소 원자의 기를 지칭한다.

본원에서 사용되는 바와 같이, "알키닐"은 적어도 하나의 삼중 결합을 함유하지만 선택적으로 하나 초과의 삼중 결합을 함유하고 추가로 선택적으로 하나 이상의 이중 결합 모이어티를 함유하는 직쇄 또는 분지쇄의 2 내지 12개의 탄소 원자의 기를 지칭한다.

본원에서 사용되는 바와 같이, "헤테로알킬", "헤테로알케닐" 및 "헤테로알키닐"은 하나 이상의 탄소 원자가 동일하거나 상이한 헤테로원자 또는 헤테로원자성 기로 각각 독립적으로 대체된, 본원에서 정의된 바와 같은 알킬, 알케닐 및 알키닐을 지칭한다. 탄소 원자를 대체할 수 있는 헤테로원자 및/또는 헤테로원자성 기는 -O-, -S-, -S-O-, -NRα-, -PH-, -S(O)-, -S(O)₂-, -S(O)NRα-, -S(O)₂NRα- 등, 및 이들의 조합을 포함하지만 이로 제한되지 않고, 여기서 각각의 Rα는 수소, 알킬, 헤테로알킬, 시클로알킬, 헤테로시클로알킬, 아릴, 및 헤테로아릴로부터 독립적으로 선택된다.

본원에서 사용되는 바와 같이, "알콕시"는 기 -ORβ을 지칭하며, 여기서 Rβ는 본원에서 또한 정의된 바와 같은 선택적으로 치환된 알킬기를 포함하는 상기 정의된 바와 같은 알킬기이다.

본원에서 사용되는 바와 같이, "아릴"은 단일 고리(예를 들어, 페닐) 또는 다중 축합 고리(예를 들어, 나프틸 또는 안트릴)를 갖는 6 내지 12개의 탄소 원자의 불포화 방향족 카르보시클릭기를 지칭한다. 예시적인 아릴은 페닐, 피리딜, 나프틸 등을 포함한다.

본원에서 사용되는 바와 같이, "아미노"는 기 -NH₂를 지칭한다. 치환된 아미노는 기 -NHRδ, NRδRδ 및 NRδRδRδ를 지칭하며, 여기서 각각의 Rδ는 치환 또는 비치환된 알킬, 시클로알킬, 시클로헤테로알킬, 알콕시, 아릴, 헤테로아릴, 헤테로아릴알킬, 아실, 알콕시카르보닐, 술파닐, 술피닐, 설포닐 등으로부터 독립적으로 선택된다. 전형적인 아미노기는 디메틸아미노, 디에틸아미노, 트리메틸암모늄, 트리에틸암모늄, 메틸설포닐아미노, 푸라닐-옥시-설파미노 등을 포함하지만, 이로 제한되지 않는다.

본원에서 사용되는 바와 같이, "옥소"는 =0을 지칭한다.

본원에서 사용되는 바와 같이, "옥시"는 에테르 및 에스테르를 포함하는 상이한 옥시기를 형성하기 위해 다양한 치환체를 가질 수 있는 2가 기 -O-를 지칭한다.

본원에서 사용되는 바와 같이, "카르복시"는 -COOH를 의미한다.

본원에서 사용되는 바와 같이, "카르보닐"은 산, 산 할라이드, 알데하이드, 아미드, 에스테르 및 케톤을 포함하는 상이한 카르보닐기를 형성하기 위해 다양한 치환체를 가질 수 있는 -C(O)-를 지칭한다.

본원에서 사용되는 바와 같이, "알킬옥시카르보닐"은 -C(O)ORε를 지칭하고, 여기서 Rε는 선택적으로 치환될 수 있는 본원에서 정의된 바와 같은 알킬기이다.

본원에서 사용되는 바와 같이, "아미노카르보닐"은 -C(0)NH₂를 지칭한다. 치환된 아미노카르보닐은 -C(O)NRδRδ를 지칭하며, 여기서 아미노기 NRδRδ는 본원에서 정의된 바와 같다.

본원에서 사용되는 바와 같이, "할로겐" 및 "할로"는 플루오로, 클로로, 브로 모 및 요오도를 지칭한다.

본원에서 사용되는 바와 같이, "하이드록시"는 -OH를 지칭한다.

본원에서 사용되는 바와 같이, "시아노"는 -CN을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로아릴"은 1 내지 10개의 탄소 원자 및 고리 내의 산소, 질소 및 황으로부터 선택되는 1 내지 4개의 헤테로원자의 방향족 헤테로사이클릭 기를 지칭한다. 이러한 헤테로아릴 기는 단일 고리(예를 들어, 피리딜 또는 푸릴) 또는 다중 축합 고리(예를 들어, 인돌리지닐 또는 벤조티에닐)를 가질 수 있다.

본원에서 사용되는 바와 같이, "헤테로아릴알킬"은 바람직하게는 알킬 모이어티에 1 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알킬(즉, 헤테로아릴-알킬 기)을 지칭한다. 이러한 헤테로아릴알킬 기는 피리딜메틸 등으로 예시된다.

본원에서 사용되는 바와 같이, "헤테로아릴알케닐"은 바람직하게는 알케닐 모이어티에 2 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알케닐(즉, 헤테로아릴-알케닐 기)을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로아릴알키닐"은 바람직하게는 알키닐 모이어티에 2 내지 6개의 탄소 원자 및 헤테로아릴 모이어티에 5 내지 12개의 고리 원자를 포함하는, 헤테로아릴로 치환된 알키닐(즉, 헤테로아릴-알키닐 기)을 지칭한다.

본원에서 사용되는 바와 같이, "헤테로사이클", "헤테로사이클릭" 및 상호 교환 가능하게 "헤테로사이클로알킬"은 단일 고리 또는 다중 축합 고리, 2 내지 10개의 탄소 고리 원자 및 고리 내의 질소, 황 또는 산소로부터 선택되는 1 내지 4개의 헤테로 고리 원자를 포함하는 포화 또는 불포화 기를 지칭한다. 이러한 헤테로사이클릭 기는 단일 고리(예를 들어, 피페리디닐 또는 테트라하이드로푸릴) 또는 다중 축합 고리(예를 들어, 인돌리닐, 디하이드로벤조푸란 또는 퀴누클리디닐)를 가질 수 있다. 헤테로사이클의 예는 푸란, 티오펜, 티아졸, 옥사졸, 피롤, 이미다졸, 피라졸, 피리딘, 피라진, 피리미딘, 피리다진, 인돌리진, 이소인돌, 인돌, 인다졸, 퓨린, 퀴놀리진, 이소퀴놀린, 퀴놀린, 프탈라진, 나프틸피리딘, 퀴녹살린, 퀴나졸린, 신놀린, 프테리딘, 카르바졸, 카르볼린, 페난트리딘, 아크리딘, 페난트롤린, 이소티아졸, 페나진, 이속사졸, 페녹사진, 페노티아진, 이미다졸리딘, 이미다졸린, 피페리딘, 피페라진, 피롤리딘, 인돌린을 포함하지만, 이로 제한되지 않는다.

본원에서 사용되는 바와 같이, "원 고리(membered ring)"는 임의의 시클릭 구조를 포함하는 것을 의미한다. 용어 "원" 앞의 숫자는 고리를 구성하는 백본 원자의 수를 나타낸다. 따라서, 예를 들어 시클로헥실, 피리딘, 피란 및 티오피란은 6원 고리이고, 시클로펜틸, 피롤, 푸란 및 티오펜은 5원 고리이다.

달리 명시되지 않는 한, 상기한 기에서 수소가 차지하는 위치는 하이드록시, 옥소, 니트로, 메톡시, 에톡시, 알콕시, 치환된 알콕시, 트리플루오로메톡시, 할로알콕시, 플루오로, 클로로, 브로모, 요오도, 할로, 메틸, 에틸, 프로필, 부틸, 알킬, 알케닐, 알키닐, 치환된 알킬, 트리플루오로메틸, 할로알킬, 하이드록시알킬, 알콕시알킬, 티오, 알킬티오, 아실, 카르복시, 알콕시카르보닐, 카르복사미도, 치환된 카르복사미도, 알킬설포닐, 알킬설피닐, 알킬설포닐아미노, 설폰아미도, 치환된 설폰아미도, 시아노, 아미노, 치환된 아미노, 알킬아미노, 디알킬아미노, 아미노알킬, 아실아미노, 아미디노, 아미독시모, 하이드록사모일, 페닐, 아릴, 치환된 아릴, 아릴옥시, 아릴알킬, 아릴알케닐, 아릴알키닐, 피리딜, 이미다졸릴, 헤테로아릴, 치환된 헤테로아릴, 헤테로아릴옥시, 헤테로아릴알킬, 헤테로아릴알케닐, 헤테로아릴알키닐, 사이클로프로필, 사이클로부틸, 사이클로펜틸, 사이클로헥실, 사이클로알킬, 시클로알케닐, 사이클로알킬알킬, 치환된 사이클로알킬, 사이클로알킬옥시, 피롤리디닐, 피페리디닐, 모르폴리노, 헤테로사이클, (헤테로사이클)옥시, 및 (헤테로사이클)알킬을 포함하고 이로 제한되지 않는 치환체로 추가로 치환될 수 있고; 바람직한 헤테로원자는 산소, 질소 및 황이다. 개방 원자가(open valence)가 이들 치환체에 존재하는 경우, 알킬, 사이클로알킬, 아릴, 헤테로아릴 및/또는 헤테로사이클 기로 추가로 치환될 수 있고, 이러한 개방 원자가가 탄소에 존재하는 경우 할로겐 및 산소-, 질소- 또는 황-결합 치환체에 의해 추가로 치환될 수 있음이 이해되고, 그러한 개방 원자가가 여러 개 존재하는 경우, 이들 기는 결합의 직접 형성 또는 새로운 헤테로 원자, 바람직하게는 산소, 질소 또는 황에 대한 결합의 형성에 의해 결합되어 고리를 형성할 수 있다. 수소를 치환체로 대체하는 것이 본 발명의 분자에 허용되지 않는 불안정성을 도입하지 않고, 화학적으로 합리적이라면 상기 치환이 이루어질 수 있음이 추가로 이해된다.

본원에서 사용되는 바와 같이, 용어 "배양"은 임의의 적합한 조건(예를 들어, 액체, 겔 또는 고체 배지의 사용) 하에서 미생물 세포 집단의 성장을 지칭한다.

재조합 폴리펩타이드는 관련 기술 분야에 공지된 임의의 적합한 방법을 사용하여 생산될 수 있다. 관심있는 야생형 폴리펩타이드를 코딩하는 유전자는 플라스미드와 같은 벡터에서 클로닝될 수 있고, 이. 콜라이 등과 같은 원하는 숙주에서 발현될 수 있다. 재조합 폴리펩타이드의 변이체는 관련 기술 분야에 공지된 다양한 방법에 의해 생성될 수 있다. 실제로, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려진 매우 다양한 상이한 돌연변이 유발 기술이 존재한다. 또한, 돌연변이 유발 키트는 많은 상업용 분자 생물학 공급업체에서 입수할 수 있다. 정의된 아미노산에서 특이적 치환(부위 지정), 유전자의 국소 영역에서 특이적 또는 무작위 돌연변이(영역 특이적) 또는 전체 유전자에 대한 무작위 돌연변이(예를 들어, 포화 돌연변이 유발)를 만드는 방법이 있다. PCR, 카세트 돌연변이 유발, 유전자 합성, 오류 빈발 PCR, 셔플링을 사용한 단일 가닥 DNA 또는 이중 가닥 DNA의 부위 지정 돌연변이 유발, 및 화학적 포화 돌연변이 유발, 또는 관련 기술 분야에 공지된 임의의 다른 적합한 방법을 포함하지만 이로 제한되지 않는, 효소 변이체를 생성하기 위한 많은 적합한 방법이 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다. 돌연변이 유발 및 유도 진화 방법은 발현, 스크리닝 및 분석할 수 있는 변이체 라이브러리를 생성하기 위해 효소 코딩 폴리뉴클레오타이드에 쉽게 적용될 수 있다. 임의의 적합한 돌연변이 유발 및 유도 진화 방법은 본 발명에서 유용하며, 관련 기술 분야에 잘 알려져 있다(예를 들어, 미국 특허 5,605,793, 5,811,238, 5,830,721, 5,834,252, 5,837,458, 5,928,905, 6,096,548, 6,117,679, 6,132,970, 6,165,793, 6,180,406, 6,251,674, 6,265,201, 6,277,638, 6,287,861, 6,287,862, 6,291,242, 6,297,053, 6,303,344, 6,309,883, 6,319,713, 6,319,714, 6,323,030, 6,326,204, 6,335,160, 6,335,198, 6,344,356, 6,352,859, 6,355,484, 6,358,740, 6,358,742, 6,365,377, 6,365,408, 6,368,861, 6,372,497, 6,337,186, 6,376,246, 6,379,964, 6,387,702, 6,391,552, 6,391,640, 6,395,547, 6,406,855, 6,406,910, 6,413,745, 6,413,774, 6,420,175, 6,423,542, 6,426,224, 6,436,675, 6,444,468, 6,455,253, 6,479,652, 6,482,647, 6,483,011, 6,484,105, 6,489,146, 6,500,617, 6,500,639, 6,506,602, 6,506,603, 6,518,065, 6,519,065, 6,521,453, 6,528,311, 6,537,746, 6,573,098, 6,576,467, 6,579,678, 6,586,182, 6,602,986, 6,605,430, 6,613,514, 6,653,072, 6,686,515, 6,703,240, 6,716,631, 6,825,001, 6,902,922, 6,917,882, 6,946,296, 6,961,664, 6,995,017, 7,024,312, 7,058,515, 7,105,297, 7,148,054, 7,220,566, 7,288,375, 7,384,387, 7,421,347, 7,430,477, 7,462,469, 7,534,564, 7,620,500, 7,620,502, 7,629,170, 7,702,464, 7,747,391, 7,747,393, 7,751,986, 7,776,598, 7,783,428, 7,795,030, 7,853,410, 7,868,138, 7,783,428, 7,873,477, 7,873,499, 7,904,249, 7,957,912, 7,981,614, 8,014,961, 8,029,988, 8,048,674, 8,058,001, 8,076,138, 8,108,150, 8,170,806, 8,224,580, 8,377,681, 8,383,346, 8,457,903, 8,504,498, 8,589,085, 8,762,066, 8,768,871, 9,593,326, 및 모든 관련 미국, PCT 및 미국 이외의 대응 특허; Ling et al., Anal. Biochem., 254(2):157-78 [1997]; Dale et al., Meth. Mol. Biol., 57:369-74 [1996]; Smith, Ann. Rev. Genet., 19:423-462 [1985]; Botstein et al., Science, 229:1193-1201 [1985]; Carter, Biochem. J., 237:1-7 [1986]; Kramer et al., Cell, 38:879-887 [1984]; Wells et al., Gene, 34:315-323 [1985]; Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]; Christians et al., Nat. Biotechnol., 17:259-264 [1999]; Crameri et al., Nature, 391:288-291 [1998]; Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]; Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]; Crameri et al., Nat. Biotechnol., 14:315-319 [1996]; Stemmer, Nature, 370:389-391 [1994]; Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767; 및 WO 2009/152336 참조; 이들 모두는 본원에 참조로 포함됨).

일부 실시양태에서, 돌연변이 유발 처리 후 수득된 효소 클론은 효소 제제를 정의된 온도(또는 다른 분석 조건)에 적용하는 단계 및 열 처리 또는 다른 적합한 분석 조건 후 남아있는 효소 활성의 양을 측정하는 단계에 의해 스크리닝된다. 이후, 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 함유하는 클론을 유전자로부터 단리하고, 시퀀싱하여 뉴클레오타이드 서열 변화(존재하는 경우)를 확인하고, 숙주 세포에서 효소를 발현하는데 사용한다. 발현 라이브러리로부터 효소 활성을 측정하는 것은 관련 기술 분야에 공지된 임의의 적합한 방법(예를 들어, 표준 생화학 기술, 예를 들어 HPLC 분석)을 사용하여 수행될 수 있다.

변이체가 생성된 후, 이들은 임의의 원하는 특성(예를 들어, 높거나 증가된 활성, 또는 낮거나 감소된 활성, 증가된 열 활성, 증가된 열 안정성 및/또는 산성 pH 안정성 등)에 대해 스크리닝될 수 있다. 일부 실시양태에서, "재조합 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드"(본원에서 "조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드", "변이체 퓨린 뉴클레오사이드 포스포릴라제 효소", "퓨린 뉴클레오사이드 포스포릴라제 변이체" 및 "퓨린 뉴클레오사이드 포스포릴라제 조합 변이체"로도 지칭됨)가 유용하다. 일부 실시양태에서, "재조합 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드"("조작된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드", "변이체 퓨린 뉴클레오사이드 포스포릴라제 효소", "퓨린 뉴클레오사이드 포스포릴라제 변이체" 및 "퓨린 뉴클레오사이드 포스포릴라제 조합 변이체"로도 지칭됨)가 유용하다.

본원에서 사용되는 바와 같이, "벡터"는 DNA 서열을 세포에 도입하기 위한 DNA 구축물이다. 일부 실시양태에서, 벡터는 DNA 서열에서 코딩되는 폴리펩타이드의 적합한 숙주에서 발현을 수행할 수 있는 적합한 제어 서열에 작동 가능하게 연결된 발현 벡터이다. 일부 실시양태에서, "발현 벡터"는 숙주 세포에서 발현을 유도하기 위해 DNA 서열(예를 들어, 트랜스진)에 작동 가능하게 연결된 프로모터 서열을 갖고, 일부 실시양태에서 전사 종결자 서열도 포함한다.

본원에서 사용되는 바와 같이, 용어 "발현"은 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이로 제한되지 않는, 폴리펩타이드의 생산에 관련된 임의의 단계를 포함한다. 일부 실시양태에서, 상기 용어는 또한 세포로부터 폴리펩타이드의 분비를 포함한다.

본원에서 사용되는 바와 같이, 용어 "생산하다"는 세포에 의한 단백질 및/또는 다른 화합물의 생산을 지칭한다. 이 용어는 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이로 제한되지 않는, 폴리펩타이드의 생산에 관련된 임의의 단계를 포함하는 것으로 의도된다. 일부 실시양태에서, 상기 용어는 또한 세포로부터 폴리펩타이드의 분비를 포함한다.

본원에서 사용되는 바와 같이, 아미노산 또는 뉴클레오타이드 서열(예를 들어, 프로모터 서열, 신호 펩타이드, 종결자 서열 등)은 2개의 서열이 자연에서 회합되지 않는 경우, 그 서열이 작동 가능하게 연결된 또 다른 서열에 대해 "이종성"이다. 예를 들어, "이종성 폴리뉴클레오타이드"는 실험 기술에 의해 숙주 세포로 도입된 임의의 폴리뉴클레오타이드이며, 숙주 세포에서 제거되고 실험 조작을 거친 다음, 숙주 세포로 재도입되는 폴리뉴클레오타이드를 포함한다.

본원에서 사용되는 바와 같이, 용어 "숙주 세포" 및 "숙주 균주"는 본원에서 제공되는 DNA(예를 들어, 퓨린 뉴클레오사이드 포스포릴라제 변이체를 코딩하는 폴리뉴클레오타이드)를 포함하는 발현 벡터에 적합한 숙주를 지칭한다. 일부 실시양태에서, 숙주 세포는 관련 기술 분야에 공지된 바와 같은 재조합 DNA 기술을 사용하여 구축된 벡터로 형질전환 또는 형질감염된 원핵 또는 진핵 세포이다.

용어 "유사체"는 참조 폴리펩타이드와 70% 초과의 서열 동일성 내지 100% 미만의 서열 동일성(예를 들어, 75%, 78%, 80%, 83%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과의 서열 동일성)을 갖는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 호모아르기닌, 오르니틴 및 노르발린을 포함하지만 이로 제한되지 않는 하나 이상의 비자연 발생 아미노산 잔기뿐만 아니라, 자연 발생 아미노산을 함유하는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 또한 하나 이상의 D-아미노산 잔기 및 2개 이상의 아미노산 잔기 사이에 비펩타이드 결합을 포함한다.

용어 "유효량"은 원하는 결과를 생성하는데 충분한 양을 의미한다. 관련 기술 분야에서 통상의 기술을 가진 자는 통상적인 실험을 사용하여 유효량을 결정할 수 있다.

용어 "단리된" 및 "정제된"은 자연적으로 회합되는 적어도 하나의 다른 성분으로부터 제거된 분자(예를 들어, 단리된 핵산, 폴리펩타이드 등) 또는 다른 성분을 지칭하기 위해 사용된다. 용어 "정제된"은 절대적인 순도를 요구하지 않으며, 오히려 상대적인 정의로서 의도된다.

본원에서 사용되는 바와 같이, "입체선택성"은 하나의 입체이성질체가 또 다른 입체이성질체보다 화학적 또는 효소에 의한 반응에서 우선적으로 형성되는 것을 지칭한다. 입체선택성은 하나의 입체이성질체의 형성이 다른 입체이성질체보다 선호되는 경우 부분적일 수 있거나, 또는 오직 하나의 입체이성질체가 형성되는 경우 완전할 수 있다. 입체이성질체가 거울상이성질체인 경우, 입체선택성은 두 거울상이성질체의 합에서 하나의 거울상이성질체의 분율(전형적으로 백분율로서 보고됨)인 거울상이성질체 선택성으로 지칭된다. 이것은 식 [주요 거울상이성질체 - 소수 거울상이성질체]/[주요 거울상이성질체 + 소수 거울상이성질체]에 따라 계산된 거울상이성질체 과량("e.e.")으로서 일반적으로 대안적으로 관련 기술 분야에 보고된다(전형적으로 백분율로서). 입체이성질체가 부분입체이성질체인 경우, 입체선택성은 일반적으로 대안적으로 부분입체이성질체 과량("d.e.")으로 보고되는, 2개의 부분입체이성질체의 혼합물에서 하나의 부분입체이성질체의 분율(전형적으로 백분율로서 보고됨)인 부분입체선택성으로 지칭된다. 거울상이성질체 과량 및 부분입체이성질체 과량은 입체이성질체 과량의 유형이다.

본원에서 사용되는 바와 같이, "위치선택성" 및 "위치선택적 반응"은 결합 형성 또는 절단에 대한 하나의 방향이 다른 모든 가능한 방향보다 우선적으로 발생하는 반응을 지칭한다. 반응은 구별이 완전하면 완전히(100%) 위치선택적일 수 있고, 하나의 부위에서의 반응 생성물이 다른 부위에서의 반응 생성물보다 우세한 경우 실질적으로 위치선택적(적어도 75%)이거나 부분적으로 위치선택적(X%, 여기서 백분율은 관심 반응에 의존적으로 설정됨)일 수 있다.

본원에서 사용되는 바와 같이, "화학선택성"은 화학적 또는 효소에 의한 반응에서 한 생성물이 다른 생성물에 비해 우선적으로 형성됨을 의미한다.

본원에서 사용되는 바와 같이, "pH 안정성"은 처리되지 않은 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 높은 또는 낮은 pH(예를 들어, 4.5-6 또는 8 내지 12)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "열안정성"은 동일한 승온에 노출된 야생형 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 승온(예를 들어, 40-80℃)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "용매 안정성"은 동일한 농도의 동일한 용매에 노출된 야생형 효소와 비교하여 일정 시간(예를 들어, 0.5-24시간) 동안 다양한 농도(예를 들어, 5-99%)의 용매(에탄올, 이소프로필 알코올, 디메틸설폭사이드 [DMSO], 테트라하이드로푸란, 2-메틸테트라하이드로푸란, 아세톤, 톨루엔, 부틸 아세테이트, 메틸 tert- 부틸 에테르 등)에 노출된 후, 유사한 활성(예를 들어, 60% 내지 80% 초과)을 유지하는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 지칭한다.

본원에서 사용되는 바와 같이, "열 및 용매 안정성"은 열안정성이면서 용매 안정성인 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 지칭하는 것이다.

본원에서 사용되는 바와 같이, "선택적" 및 "선택적으로"는 추후 설명되는 사건 또는 상황이 발생하거나 발생하지 않을 수 있다는 것과, 상기 설명이 사건 또는 상황이 발생하는 경우 및 그것이 발생하지 않는 경우를 포함한다는 것을 의미한다. 관련 기술 분야에서 통상의 기술을 가진 자는 하나 이상의 선택적인 치환체를 함유하는 것으로 기재된 임의의 분자와 관련하여, 입체적으로 실제적이고/이거나 합성적으로 가능한 화합물만이 포함되는 것을 의미한다는 것을 이해할 것이다.

본원에서 사용되는 바와 같이, "선택적으로 치환된"은 화학적 기의 용어 또는 시리즈에서 모든 후속 변형자를 지칭한다. 예를 들어, "선택적으로 치환된 아릴알킬"이라는 용어에서, 분자의 "알킬" 부분 및 "아릴" 부분은 치환되거나 치환되지 않을 수 있으며, 일련의 "선택적으로 치환된 알킬, 사이클로알킬, 아릴 및 헤테로아릴"의 경우, 알킬, 사이클로알킬, 아릴 및 헤테로아릴 기는 다른 기와 독립적으로 치환되거나 치환되지 않을 수 있다.

발명의 상세한 설명

일부 실시양태에서, 본 발명은 MK-8591(Merck)과 같은 뉴클레오사이드 유사체의 생산에 적합한 효소를 제공한다. 본 발명은 이러한 뉴클레오사이드 유사체를 생산하기 위한 효소의 잠재적인 사용을 다루기 위해 개발되었다. 그러나, 이 접근법에 대한 한 가지 도전은 필요한 모든 중간체의 생산에 필요한 필수 기질 유사체에 대해 야생형 효소가 최적일 가능성이 없다는 것이다. 또한, 합성 경로에서 각각의 효소는 원하는 뉴클레오사이드 유사체의 합성에 사용되는 방법 및 대리 기질에 적합하도록 하기 위해 일부 조작을 필요로 한다.

일부 실시양태에서, 본 발명은 화합물 (1)로 나타낸 비-천연 뉴클레오사이드 유사체의 시험관내 효소 합성 방법을 궁극적으로 유도하는 화합물의 생산에 유용한 효소를 제공한다.

화합물 (1)

비-천연 뉴클레오사이드는 암 및 바이러스 감염의 치료를 위한 약물을 비롯한 많은 중요한 약물 클래스의 필수 구성 요소이다. 적어도 12개의 뉴클레오사이 유사 약물이 시판 중이거나 임상 시험 중에 있다(Jordheim et al., Nat. Rev. Drug Discovery 12:447-464 [2013]). 화합물 (1)을 제조하는 한 가지 방법은 반응식 I에 나타낸 바와 같이 에티닐 리보스-1-포스페이트, 화합물 (3), 및 플루오로아데닌, 화합물 (2)의 퓨린 뉴클레오사이드 포스포릴라제(PNP)에 의해 촉매된 커플링에 의한 것이다.

반응식 I. 퓨린 뉴클레오사이드 포스포릴라제(PNP)에 의해 촉매된 반응

화합물 (3)과 같은 데옥시리보스-1-포스페이트 화합물은 제조하기 어려울 수 있다. 그러나, 상응하는 데옥시리보스-5-포스페이트 화합물은 효소 2-데옥시리보스-5-포스페이트 알돌라제(DERA)에 의해 촉매된 아세트알데하이드 및 D-글리세르알데하이드-3-포스페이트(또는 이들의 유사체)의 커플링을 통해 제조될 수 있다(Barbas et al., J. Am. Chem. Soc. 112:2013-2014 [1990]). 일단 데옥시리보스-1-포스페이트 유사체가 형성되면, 이것은 반응식 II에 제시된 바와 같이 효소 포스포펜토뮤타제(PPM)의 작용에 의해 반응식 I에 필요한 상응하는 데옥시리보스-5-포스페이트 유사체로 전환되거나 이성질화될 수 있다.

반응식 II

반응식 I에 제시된 PNP 및 PPM 반응의 평형 위치는 일반적으로 생성물(화합물 1 및 무기 포스페이트)이 아닌 반응물(화합물 2 및 4)에 유리하다. 반응을 더 높은 전환으로 유도하는 한 가지 방법은 커플링 단계에서 형성된 무기 포스페이트를 제거하는 것이다. 이것은 효소 수크로스 포스포릴라제(SP)에 의해 촉매된, 수크로스와 같은 디사카라이드와 무기 포스페이트를 반응시킴으로써 달성될 수 있다(예를 들어, 미국 특허 제7,229,797호 참조). 글루코스-1-포스페이트 및 프럭토스를 생성하는 상기 반응은 매우 유리하며, 아래의 반응식 III에 제시된 바와 같이 전체 반응을 유도할 수 있다.

반응식 III. 화합물 (1)을 생성하기 위한 전체 반응식

퓨린 뉴클레오사이드 포스포릴라제(PNP) 효소는 특히 이. 콜라이(Xie, Xixian et al., Biotechnol Lett 33: 1107-1112 [2011], Lee et al., Protein Expr. Purif. 22:180-188 [2001]), 바실러스 서브틸리스(Bacillus subtilis) 168, 슈도모알테로모나스(Pseudoalteromonas) 종 XM2107(Xie, Xixian et al., Biotechnol Lett 33: 1107-1112 [2011]), 바실러스 할로두란스(Bacillus halodurans) Alk36(Visser et al., Extremophiles 14:185-192 [2010]), 플라스모디움 팔시파룸(Plasmodium falciparum)(Schnick et al., Acta Cryst. D 61:1245-1254 [2005]), 및 인간(Silva et al., Protein Expr. Purif. 27:158-164 [2003])을 비롯한 여러 공급원으로부터 단리되고/되거나, 재조합 방식으로 발현되었다. 또한, 이. 콜라이(Mao et al., Structure 5:1373-1383 [1997]) 및 인간(Canduri et al., Biochem. Biophys. Res. Commun. 26:335-338 [2005])으로부터 얻은 것과 같은 여러 PNP의 결정 구조도 사용할 수 있다. 이들 효소는 (2-데옥시)퓨린 뉴클레오사이드의 유리 염기 및 (2'-데옥시)리보스-1-포스페이트로의 가역적인 인산화를 촉매한다. 6-옥소퓨린 뉴클레오사이드에 특이적인 삼량체 형태는 고급 유기체 및 원핵생물에서 발견되는 반면, 6-옥소 및 6-아미노 퓨린 뉴클레오사이드 둘 모두에 대해 활성인 육량체 형태는 저급 유기체에서만 발견된다(Bennett et al., J Biol Chem 278:47110-47118 [2003]). 야생형 PNP 효소의 활성은 비-천연 뉴클레오사이드에 대해 입증되었지만(Schnick et al., Acta Cryst. D 61:1245-1254 [2005], Visser et al., Extremophiles 14:185-192 [2010], Birmingham et al., Nat. Chem. Biol. 10:392-399 [2014]), 일반적으로 화합물 (1)과 같은 많은 비-천연 뉴클레오사이드를 상업적인 양으로 생산하기에는 충분하지 않다.

비-천연 및 치료상 유용한 뉴클레오사이드를 제조하기 위한 비-천연 기질에 대한 PNP의 불량한 활성으로 인해, 개선된 활성을 갖고 전형적인 산업 조건 하에서 작동할 수 있는 조작된 PNP에 대한 필요성이 존재한다. 본 발명은 이러한 요구를 해결하고, 산업 조건 하에서 이러한 반응에 사용하기 적합한 조작된 PNP를 제공한다.

조작된 PNP 폴리펩타이드

본 발명은 조작된 PNP 폴리펩타이드, 상기 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 상기 폴리펩타이드의 제조 방법 및 상기 폴리펩타이드의 사용 방법을 제공한다. 설명이 폴리펩타이드에 관한 경우, 그 설명은 폴리펩타이드를 코딩하는 폴리뉴클레오타이드도 설명하는 것으로 이해되어야 한다. 일부 실시양태에서, 본 발명은 야생형 PNP 효소에 비해 개선된 특성을 갖는 조작된 비-자연 발생 PNP 효소를 제공한다. 임의의 적합한 반응 조건이 본 발명에서 유용하다. 일부 실시양태에서, 이성질화 반응을 수행하기 위해 조작된 폴리펩타이드의 개선된 특성을 분석하기 위한 방법이 사용된다. 일부 실시양태에서, 반응 조건은 아래에서 및 실시에서 추가로 설명되는 바와 같이 조작된 PNP의 농도 또는 양, 기질(들), 완충제(들), 용매(들), pH, 온도 및 반응 시간을 포함하는 조건, 및/또는 고체 지지체 상에 고정된 조작된 PNP 폴리펩타이드에 대한 조건과 관련하여 변경된다.

일부 실시양태에서, 추가의 반응 성분 또는 추가의 기술이 반응 조건을 보충하기 위해 사용된다. 일부 실시양태에서, 이들은 효소를 안정화하거나 불활성화를 방지하고, 생성물 억제를 감소시키고, 반응 평형을 원하는 생성물 형성으로 이동시키기 위한 조치를 취하는 것을 포함한다.

일부 추가의 실시양태에서, 기질 화합물을 생성물 화합물로 전환하기 위한 상기 기재된 임의의 공정은 다음으로부터 선택된 하나 이상의 단계를 추가로 포함할 수 있다: 생성물 화합물(들)의 추출, 단리, 정제, 결정화, 여과 및/또는 동결건조. 본원에서 제공되는 공정에 의해 생산된 생촉매 반응 혼합물로부터 생성물(들)을 추출, 단리, 정제 및/또는 결정화하기 위한 방법, 기술 및 프로토콜은 통상의 기술자에게 공지되어 있고/있거나, 통상적인 실험을 통해 실시된다. 추가로, 예시적인 방법은 하기 실시예에서 제공된다.

조작된 폴리펩타이드를 코딩하는 조작된 PNP 폴리뉴클레오타이드, 발현 벡터 및 숙주 세포

본 발명은 본원에서 설명되는 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 폴리펩타이드를 발현할 수 있는 재조합 폴리뉴클레오타이드를 생성하기 위해 유전자 발현을 제어하는 하나 이상의 이종성 조절 서열에 작동 가능하게 연결될 수 있다. 일부 실시양태에서, 조작된 효소 폴리펩타이드(들)를 코딩하는 적어도 하나의 이종성 폴리뉴클레오타이드를 함유하는 발현 구축물은 상응하는 효소 폴리펩타이드(들)를 발현하기 위해 적절한 숙주 세포 내로 도입된다.

통상의 기술자에게 명백할 바와 같이, 단백질 서열의 이용 가능성 및 다양한 아미노산에 상응하는 코돈에 대한 지식은 대상 폴리펩타이드를 코딩할 수 있는 모든 폴리뉴클레오타이드에 대한 설명을 제공한다. 동일한 아미노산이 대체 또는 동의 코돈에 의해 코딩되는 유전자 코드의 축퇴성은 극히 많은 수의 핵산이 제조될 수 있도록 하며, 이들 모두는 조작된 효소(예를 들어, PNP) 폴리펩타이드를 코딩한다. 따라서, 본 발명은 가능한 코돈 선택에 기초하여 조합을 선택함으로써 본원에서 설명되는 효소 폴리펩타이드를 코딩하는 효소 폴리뉴클레오타이드의 각각의 및 모든 가능한 변이를 생성하기 위한 방법 및 조성물을 제공하고, 모든 상기 변이는 실시예에서(예를 들어, 다양한 표에서) 제시되는 아미노산 서열을 포함하는, 본원에서 설명되는 임의의 폴리펩타이드에 대해 구체적으로 개시된 것으로 간주될 것이다.

일부 실시양태에서, 코돈은 바람직하게는 단백질 생산을 위해 선택된 숙주 세포에 의한 이용을 위해 최적화된다. 예를 들어, 박테리아에서 사용되는 선호 코돈은 일반적으로 박테리아에서의 발현을 위해 사용된다. 결과적으로, 조작된 효소 폴리펩타이드를 코딩하는 코돈 최적화된 폴리뉴클레오타이드는 전장 코딩 영역에서 코돈 위치의 약 40%, 50%, 60%, 70%, 80%, 90% 또는 90% 초과의 위치에 바람직한 코돈을 포함한다.

일부 실시양태에서, 효소 폴리뉴클레오타이드는 본원에서 개시되는 특성을 갖는 효소 활성을 갖는 조작된 폴리펩타이드를 코딩하고, 여기서 폴리펩타이드는 본원에서 제공되는 서열 번호로부터 선택되는 참조 서열에 대해 적어도 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 동일성을 갖는 아미노산 서열, 또는 임의의 변이체의 아미노산 서열(예를 들어, 실시예에서 제공되는 변이체), 및 참조 폴리뉴클레오타이드(들)과 비교할 때 하나 이상의 잔기 차이, 또는 실시예에서 개시되는 임의의 변이체의 아미노산 서열(예를 들어, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10개 또는 그 초과의 아미노산 잔기 위치)을 포함한다. 일부 실시양태에서, 참조 폴리펩타이드 서열은 서열 번호 2, 6, 126, 242, 및 684로부터 선택된다.

일부 실시양태에서, 폴리뉴클레오타이드는 본원에서 제공되는 임의의 폴리뉴클레오타이드 서열, 또는 그의 상보체로부터 선택된 참조 폴리뉴클레오타이드 서열, 또는 본원에서 제공되는 임의의 변이체 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열에 매우 엄격한 조건 하에서 혼성화할 수 있다. 일부 실시양태에서, 매우 엄격한 조건 하에서 혼성화할 수 있는 폴리뉴클레오타이드는 참조 서열과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함하는 효소 폴리펩타이드를 코딩한다.

일부 실시양태에서, 본원에서 제공되는 임의의 조작된 효소 폴리펩타이드를 코딩하는 단리된 폴리뉴클레오타이드는 효소 폴리펩타이드의 발현을 촉진하기 위해 다양한 방식으로 조작된다. 일부 실시양태에서, 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 하나 이상의 제어 서열이 효소 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 조절하기 위해 존재하는 발현 벡터를 포함한다. 단리된 폴리뉴클레오타이드를 벡터 내로 삽입하기 전에 조작하는 것은 이용되는 발현 벡터에 따라 바람직하거나 필요할 수 있다. 재조합 DNA 방법을 이용하여 폴리뉴클레오타이드 및 핵산 서열을 변형시키기 위한 기술은 관련 기술 분야에 널리 공지되어 있다. 일부 실시양태에서, 제어 서열은 다른 서열 중에서, 프로모터, 리더 서열, 폴리아데닐화 서열, 프로펩타이드 서열, 신호 펩타이드 서열, 및 전사 종결자를 포함한다. 일부 실시양태에서, 적합한 프로모터는 선택된 숙주 세포에 기초하여 선택될 수 있다. 박테리아 숙주 세포의 경우, 본 발명의 핵산 구축물의 전사를 지시하기 위한 적합한 프로모터는 이. 콜라이 lac 오페론, 스트렙토마이세스 코엘리컬러(Streptomyces coelicolor) 아가라아제 유전자(dagA), 바실러스 서브틸리스(Bacillus subtilis) 레반수크라아제 유전자(sacB), 바실러스 리케니포르미스(Bacillus licheniformis) 알파-아밀라아제 유전자(amyL), 바실러스 스테아로써모필러스(Bacillus stearothermophilus) 말토제닉(maltogenic) 아밀라아제 유전자(amyM), 바실러스 아밀로리퀘파시엔스(Bacillus amyloliquefaciens) 알파-아밀라아제 유전자(amyQ), 바실러스 리케니포르미스 페니실리나아제 유전자(penP), 바실러스 서브틸리스 xylA 및 xylB 유전자, 및 원핵 베타-락타마아제 유전자로부터 수득된 프로모터(예를 들어, 문헌 [Villa-Kamaroff et al., Proc. Natl Acad. Sci .USA 75: 3727-3731 [1978]] 참조)뿐만 아니라, tac 프로모터(예를 들어, 문헌 [DeBoer et al., Proc. Natl Acad. Sci. USA 80: 21-25 [1983] 참조)를 포함하지만, 이로 제한되지 않는다. 사상형 진균 숙주 세포를 위한 예시적인 프로모터는 아스퍼질러스 오리재(Aspergillus oryzae) TAKA 아밀라아제, 리조무코르 미에헤이(Rhizomucor miehei) 아스파르틱 프로테이나아제, 아스퍼질러스 니거(Aspergillus niger) 중성 알파-아밀라아제, 아스퍼질러스 니거 산 안정성 알파-아밀라아제, 아스퍼질러스 니거 또는 아스퍼질러스 아와모리(Aspergillus awamori) 글루코 아밀라아제(glaA), 리조무코르 미에헤이 리파아제, 아스퍼질러스 오리재 알칼리성 프로테아제, 아스퍼질러스 오리재 트리오스 포스페이트 이소머라아제, 아스퍼질러스 니둘란스(Aspergillus nidulans) 아세트아미다아제, 및 푸사리움 옥시스포룸(Fusarium oxysporum) 트립신-유사 프로테아제(예를 들어, WO 96/00787 참조)에 대한 유전자로부터 수득된 프로모터뿐만 아니라, NA2-tpi 프로모터(아스퍼질러스 니거 중성 알파-아밀라아제 및 아스퍼질러스 오리재 트리오스 포스페이트 이소머라아제에 대한 유전자로부터의 프로모터의 혼성체), 및 이의 돌연변이체 프로모터, 말단 절단된 프로모터, 및 혼성체 프로모터를 포함하지만, 이로 제한되지 않는다. 예시적인 효모 세포 프로모터는 사카로마이세스 세레비지애 에놀라아제(ENO-1), 사카로마이세스 세레비지애 갈락토키나아제(GAL1), 사카로마이세스 세레비지애 알코올 데히드로게나제/글리세르알데하이드-3-포스페이트 데히드로게나제(ADH2/GAP), 및 사카로마이세스 세레비지애 3-포스포글리세레이트 키나아제에 대한 유전자로부터 유래될 수 있다. 효모 숙주 세포를 위한 다른 유용한 프로모터는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., Yeast 8:423-488 [1992]] 참조).

일부 실시양태에서, 제어 서열은 적합한 전사 종결자 서열(즉, 전사를 종결시키기 위해 숙주 세포에 의해 인식되는 서열)이다. 일부 실시양태에서, 종결자 서열은 효소 폴리펩타이드를 코딩하는 핵산 서열의 3' 말단에 작동 가능하게 연결된다. 선택되는 숙주 세포에서 기능적인 임의의 적어도 종결자가 본 발명에서 사용된다. 사상형 진균 숙주 세포를 위한 예시적인 전사 종결자는 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 아스퍼질러스 니둘란스 안트라닐레이트 합성효소, 아스퍼질러스 니거 알파-글루코시다아제, 및 푸사리움 옥시스포룸 트립신-유사 프로테아제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 예시적인 종결자는 사카로마이세스 세레비지애 에놀라아제, 사카로마이세스 세레비지애 시토크롬 C(CYC1), 및 사카로마이세스 세레비지애 글리세르알데하이드-3-포스페이트 데히드로게나제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 다른 유용한 종결자는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., 상기 문헌] 참조).

일부 실시양태에서, 제어 서열은 적합한 리더 서열(즉, 숙주 세포에 의한 번역에 중요한 mRNA의 비번역된 영역)이다. 일부 실시양태에서, 리더 서열은 효소 폴리펩타이드를 코딩하는 핵산 서열의 5' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능적인 임의의 적합한 리더 서열이 본 발명에서 사용될 수 있다. 사상형 진균 숙주 세포에 대한 예시적인 리더는 아스퍼질러스 오리재 TAKA 아밀라아제 및 아스퍼질러스 니둘란스 트리오스 포스페이트 이소머라아제에 대한 유전자로부터 수득된다. 효모 숙주 세포에 대한 적합한 리더는 사카로마이세스 세레비지애 에놀라아제(ENO-1), 사카로마이세스 세레비지애 3-포스포글리세레이트 키나아제, 사카로마이세스 세레비지애 알파-인자, 및 사카로마이세스 세레비지애 알코올 데히드로게나제/글리세르알데하이드-3-포스페이트 데히드로게나제(ADH2/GAP)에 대한 유전자로부터 수득된다.

일부 실시양태에서, 제어 서열은 폴리아데닐화 서열(즉, 핵산 서열의 3' 말단에 작동 가능하게 연결되고, 전사될 때 전사되는 mRNA에 폴리아데노신 잔기를 부가하기 위한 신호로서 숙주 세포에 의해 인식되는 서열)이다. 선택된 숙주 세포에서 기능적인 임의의 적합한 폴리아데닐화 서열이 본 발명에 사용될 수 있다. 사상형 진균 숙주 세포에 대한 예시적인 폴리아데닐화 서열은 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 아스퍼질러스 니둘란스 안트라닐레이트 합성효소, 푸사리움 옥시스포룸 트립신-유사 프로테아제, 및 아스퍼질러스 니거 알파-글루코시다아제에 대한 유전자를 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 대한 유용한 폴리아데닐화 서열은 공지되어 있다(예를 들어, 문헌 [Guo and Sherman, Mol. Cell. Bio., 15:5983-5990 [1995] 참조).

일부 실시양태에서, 제어 서열은 또한 신호 펩타이드(즉, 폴리펩타이드의 아미노 말단에 연결된 아미노산 서열을 코딩하고 코딩되는 폴리펩타이드를 세포의 분비 경로로 유도하는 코딩 영역)이다. 핵산 서열의 코딩 서열의 5' 말단은 분비된 폴리펩타이드를 코딩하는 코딩 영역의 세그먼트에 번역 리딩 프레임으로 자연적으로 연결된 신호 펩타이드 코딩 영역을 본질적으로 함유할 수 있다. 대안적으로, 일부 실시양태에서, 코딩 서열의 5' 말단은 코딩 서열에 대해 외래 물질인 신호 펩타이드 코딩 영역을 함유한다. 발현된 폴리펩타이드를 선택된 숙주 세포의 분비 경로 내로 유도하는 임의의 적합한 신호 펩타이드 코딩 영역이 조작된 폴리펩타이드(들)의 발현에 사용될 수 있다. 박테리아 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 바실러스 NClB 11837 말토제닉 아밀라아제, 바실러스 스테아로써모필러스 알파-아밀라아제, 바실러스 리케니포르미스 서브틸리신, 바실러스 리케니포르미스 베타-락타마아제, 바실러스 스테아로써모필러스 중성 프로테아제(nprT, nprS, nprM), 및 바실러스 서브틸리스 prsA에 대한 유전자로부터 수득된 것을 포함하지만 이로 제한되지 않는 신호 펩타이드 코딩 영역이다. 추가의 신호 펩타이드가 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Simonen and Palva, Microbiol. Rev., 57:109-137 [1993] 참조). 일부 실시양태에서, 사상형 진균 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 아스퍼질러스 오리재 TAKA 아밀라아제, 아스퍼질러스 니거 중성 아밀라아제, 아스퍼질러스 니거 글루코아밀라아제, 리조무코르 미에헤이 아스파르틱 프로테이나아제, 후미콜라 인솔렌스(Humicola insolens) 셀룰라아제, 및 후미콜라 라누기노사(Humicola lanuginosa) 리파아제에 대한 유전자로부터 수득된 신호 펩타이드 코딩 영역을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 대한 유용한 신호 펩타이드는 사카로마이세스 세레비지애 알파-인자 및 사카로마이세스 세레비지애 인버타아제에 대한 유전자로부터의 것을 포함하지만, 이로 제한되지 않는다.

일부 실시양태에서, 제어 서열은 폴리펩타이드의 아미노 말단에 위치한 아미노산 서열을 코딩하는 프로펩타이드 코딩 영역이다. 생성된 폴리펩타이드는 "프로효소", "프로폴리펩타이드" 또는 "자이모겐(zymogen)"으로 지칭된다. 프로폴리펩타이드는 프로폴리펩타이드로부터 프로펩타이드의 촉매에 의한 또는 자가촉매에 의한 절단에 의해 성숙한 활성 폴리펩타이드로 전환될 수 있다. 프로펩타이드 코딩 영역은 바실러스 서브틸리스 알칼리성 프로테아제(aprE), 바실러스 서브틸리스 중성 프로테아제(nprT), 사카로마이세스 세레비지애 알파-인자, 리조무코르 미에헤이 아스파르틱 프로티나아제, 및 미셀리오프토라 써모필라(Myceliophthora thermophila) 락타아제에 대한 유전자를 포함하지만, 이로 제한되지 않는다(예를 들어, WO 95/33836 참조). 신호 펩타이드 및 프로펩타이드 영역 모두가 폴리펩타이드의 아미노 말단에 존재하는 경우, 프로펩타이드 영역은 폴리펩타이드의 아미노 말단 다음에 위치하고, 신호 펩타이드 영역은 프로펩타이드 영역의 아미노 말단 다음에 위치한다.

일부 실시양태에서, 조절 서열이 또한 이용된다. 이들 서열은 숙주 세포의 성장과 관련하여 폴리펩타이드의 발현의 조절을 용이하게 한다. 조절 시스템의 예는 유전자의 발현이 조절 화합물의 존재를 포함하는 화학적 또는 물리적 자극에 반응하여 켜지거나 꺼지게 하는 것이다. 원핵 숙주 세포에서, 적합한 조절 서열은 lac, tac, 및 trp 오퍼레이터(operator) 시스템을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에서, 적합한 조절 시스템은 ADH2 시스템 또는 GAL1 시스템을 포함하지만, 이로 제한되지 않는다. 사상형 진균에서, 적합한 조절 서열은 TAKA 알파-아밀라아제 프로모터, 아스퍼질러스 니거 글루코아밀라아제 프로모터, 및 아스퍼질러스 오리재 글루코아밀라아제 프로모터를 포함하지만, 이로 제한되지 않는다.

또 다른 측면에서, 본 발명은 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 및 이들이 도입되는 숙주의 유형에 따라 하나 이상의 발현 조절 영역, 예를 들어 프로모터 및 종결자, 복제 기점 등을 포함하는 재조합 발현 벡터에 관한 것이다. 일부 실시양태에서, 본원에서 설명되는 다양한 핵산 및 제어 서열은 함께 연결되어, 하나 이상의 편리한 제한 부위에 효소 폴리펩타이드를 코딩하는 핵산 서열의 삽입 또는 치환을 허용하는 상기 제한 부위를 포함하는 재조합 발현 벡터를 생산한다. 대안적으로, 일부 실시양태에서, 본 발명의 핵산 서열은 핵산 서열 또는 상기 서열을 포함하는 핵산 구축물을 발현을 위한 적절한 벡터 내로 삽입함으로써 발현된다. 발현 벡터의 생성을 수반하는 일부 실시양태에서, 코딩 서열은 발현을 위한 적절한 제어 서열과 작동 가능하게 연결되도록 벡터에 위치한다.

재조합 발현 벡터는 재조합 DNA 절차를 편리하게 거칠 수 있고 효소 폴리뉴클레오타이드 서열의 발현을 유도할 수 있는 임의의 적합한 벡터(예를 들어, 플라스미드 또는 바이러스)일 수 있다. 벡터의 선택은 전형적으로 벡터가 도입되는 숙주 세포에 대한 벡터의 적합성에 따라 결정될 것이다. 벡터는 선형 또는 폐쇄 원형 플라스미드일 수 있다.

일부 실시양태에서, 발현 벡터는 자율 복제 벡터(즉, 플라스미드, 염색체외 요소, 미니염색체, 또는 인공 염색체와 같이, 그의 복제가 염색체 복제와는 무관한 염색체외 실체로서 존재하는 벡터)이다. 벡터는 자가 복제를 보장하기 위한 임의의 수단을 함유할 수 있다. 일부 대안적인 실시양태에서, 벡터는 숙주 세포 내로 도입될 때 게놈 내로 통합되고 그것이 통합된 염색체(들)와 함께 복제되는 벡터이다. 또한, 일부 실시양태에서, 숙주 세포의 게놈 내로 도입되는 총 DNA를 함께 함유하는 단일 벡터 또는 플라스미드 또는 2개 이상의 벡터 또는 플라스미드, 또는 트랜스포존이 사용될 수 있다.

일부 실시양태에서, 발현 벡터는 형질전환된 세포의 쉬운 선별을 허용하는 하나 이상의 선별 가능 마커를 함유한다. "선별 가능 마커"는 그의 생성물이 살생물제 또는 바이러스 내성, 중금속에 대한 내성, 영양요구성(auxotroph)에 대한 원영양성(prototrophy) 등을 제공하는 유전자이다. 박테리아 선별 가능 마커의 예는 바실러스 서브틸리스 또는 바실러스 리케니포르미스로부터의 dal 유전자, 또는 암피실린, 카나마이신, 클로람페니콜 또는 테트라사이클린 내성과 같은 항생제 내성을 부여하는 마커를 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포를 위한 적합한 마커는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1, 및 URA3을 포함한다. 사상형 진균 숙주 세포에서 사용하기 위한 선별 가능 마커는 amdS(아세트아미다아제; 예를 들어 에이. 니둘란스 또는 에이. 오리재로부터의 것), argB(오르니틴 카르바모일트랜스퍼라제), bar(포스피노트리신 아세틸트랜스퍼라제; 예를 들어 에스. 하이그로스코피쿠스(S. hygroscopicus)), hph(하이그로마이신 포스포트랜스퍼라제), niaD(니트레이트 리덕타제), pyrG(오로티딘-5'-포스페이트 데카르복실라아제; 예를 들어 에이. 니둘란스 또는 에이. 오리재로부터의 것), sC(설페이트 아데닐트랜스퍼라제), 및 trpC(안트라닐레이트 합성효소)뿐만 아니라, 이들의 등가물을 포함하지만, 이로 제한되지 않는다.

또 다른 측면에서, 본 발명은 본 발명의 적어도 하나의 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하며, 상기 폴리뉴클레오타이드(들)는 숙주 세포에서 조작된 효소(들)의 발현을 위해 하나 이상의 제어 서열에 작동 가능하게 결합된다. 본 발명의 발현 벡터에 의해 코딩되는 폴리펩타이드를 발현하는데 사용하기 위한 적합한 숙주 세포는 관련 기술 분야에 널리 알려져 있으며, 박테리아 세포, 예를 들어 이. 콜라이, 비브리오 플루비알리스(Vibrio fluvialis), 스트렙토마이세스 및 살모넬라 티피무리움(Salmonella typhimurium) 세포; 진균 세포, 예를 들어 효모 세포(예를 들어, 사카로마이세스 세레비지애 및 피키아 파스토리스(Pichia pastoris) [ATCC 수탁 번호 201178]); 곤충 세포, 예를 들어 초파리(Drosophila) S2 및 스포도프테라(Spodoptera) Sf9 세포; 동물 세포, 예를 들어 CHO, COS, BHK, 293, 및 보우스 흑색종(Bowes melanoma) 세포; 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 예시적인 숙주 세포는 에셔리키아 콜라이 균주(예를 들어, W3110(△fhuA) 및 BL21)이다. 박테리아 선별 가능 마커의 예는 바실러스 서브틸리스 또는 바실러스 리케니포르미스로부터의 dal 유전자, 또는 암피실린, 카나마이신, 클로람페니콜 및/또는 테트라사이클린 내성과 같은 항생제 내성을 부여하는 마커를 포함하지만, 이로 제한되지 않는다.

일부 실시양태에서, 본 발명의 발현 벡터는 벡터를 숙주 세포의 게놈 내로의 통합 또는 게놈과 무관한 세포 내에서의 벡터의 자율 복제를 허용하는 요소(들)를 함유한다. 숙주 세포 게놈 내로의 통합을 포함하는 일부 실시양태에서, 벡터는 폴리펩타이드를 코딩하는 핵산 서열 또는 상동성 또는 비상동성 재조합에 의해 벡터를 게놈 내로 통합하기 위한 벡터의 임의의 다른 요소에 의존한다.

일부 대안적 실시양태에서, 발현 벡터는 상동성 재조합에 의해 숙주 세포를 게놈 내로 통합하도록 지시하기 위한 추가의 핵산 서열을 포함한다. 추가의 핵산 서열은 벡터가 염색체(들) 내의 정확한 위치(들)에서 숙주 세포 게놈 내로 통합될 수 있게 한다. 정확한 위치에서 통합 가능성을 높이기 위해, 통합 요소는 바람직하게는 100 내지 10,000개의 염기쌍, 바람직하게는 400 내지 10,000개의 염기쌍, 가장 바람직하게는 800 내지 10,000개의 염기쌍과 같은 충분한 수의 뉴클레오타이드를 포함하며, 이는 상동성 재조합 확률을 높이기 위해 상응하는 표적 서열에 대한 상동성이 높다. 통합 요소는 숙주 세포의 게놈 내의 표적 서열에 대해 상동성인 임의의 서열일 수 있다. 더욱이, 통합 요소는 핵산 서열을 코딩하지 않거나 코딩할 수 있다. 다른 한편으로, 벡터는 비-상동성 재조합에 의해 숙주 세포의 게놈 내에 통합될 수 있다.

자율 복제의 경우, 벡터는 벡터가 해당 숙주 세포에서 자율적으로 복제할 수 있도록 하는 복제 기점을 추가로 포함할 수 있다. 박테리아 복제 기점의 예는 P15A ori 또는 플라스미드 pBR322, pUC19, pACYC177(플라스미드가 P15A ori를 가짐) 또는 이. 콜라이에서 복제를 허용하는 pACYC184 및 바실러스에서 복제를 허용하는 pUB110, pE194 또는 pTA1060의 복제 기점이다. 효모 숙주 세포에서 사용하기 위한 복제 기점의 예는 2 미크론 복제 기점, ARS1, ARS4, ARS1과 CEN3의 조합, ARS4와 CEN6의 조합이다. 복제 기점은 그 기능을 숙주 세포에서 온도에 민감하게 만드는 돌연변이를 갖는 것일 수 있다(예를 들어, 문헌 [Ehrlich, Proc. Natl. Acad. Sci. USA 75:1433 [1978] 참조).

일부 실시양태에서, 본 발명의 핵산 서열의 하나 초과의 카피가 숙주 세포에 삽입되어 유전자 산물의 생산을 증가시킨다. 핵산 서열의 카피 수의 증가는 서열의 적어도 하나의 추가의 카피를 숙주 세포 게놈에 통합하거나 또는 증폭 가능한 선별 가능 마커 유전자를 핵산 서열과 함께 포함시킴으로써 얻을 수 있으며, 여기서 선별 가능한 마커 유전자의 증폭된 카피, 및 이에 따라 핵산 서열의 추가의 카피를 함유하는 세포는 적절한 선별 가능 제제의 존재 하에 세포를 배양함으로써 선별될 수 있다.

본 발명에서 사용하기 위한 많은 발현 벡터는 상업적으로 이용 가능하다. 적합한 상업적 발현 벡터는 포유동물 숙주 세포에서의 발현을 위한 CMV 프로모터 및 hGH 폴리아데닐화 부위 및 이. 콜라이에서의 증폭을 위한 pBR322 복제 기점 및 암피실린 내성 마커를 포함하는 p3xFLAGTM™ 발현 벡터(Sigma-Aldrich Chemicals)를 포함하지만, 이로 제한되지 않는다. 다른 적합한 발현 벡터는 pBluescriptII SK(-) 및 pBK-CMV(Stratagene), 및 pBR322(Gibco BRL), pUC(Gibco BRL), pREP4, pCEP4(Invitrogen) 또는 pPoly에서 유래된 플라스미드를 포함하지만, 이로 제한되지 않는다(예를 들어, 문헌 [Lathe et al., Gene 57:193-201 [1987]] 참조).

따라서, 일부 실시양태에서, 적어도 하나의 변이체 퓨린 뉴클레오사이드 포스포릴라제를 코딩하는 서열을 포함하는 벡터는 벡터의 증식 및 변이체 퓨린 뉴클레오사이드 포스포릴라제(들)의 발현을 허용하기 위해 숙주 세포로 형질전환된다. 일부 실시양태에서, 변이체 퓨린 뉴클레오사이드 포스포릴라제는 번역 후에 변형되어 신호 펩타이드를 제거하고, 일부 경우에 분비 후에 절단될 수 있다. 일부 실시양태에서, 상기 설명된 형질전환된 숙주 세포는 변이체 퓨린 뉴클레오사이드 포스포릴라제(들)의 발현을 허용하는 조건 하에 적합한 영양 배지에서 배양된다. 적절한 보충제를 함유하는 최소 또는 복합 배지를 포함하지만 이로 제한되지 않는, 숙주 세포 배양에 유용한 임의의 적합한 배지가 본 발명에서 유용하다. 일부 실시양태에서, 숙주 세포는 HTP 배지에서 성장된다. 적절한 배지는 다양한 상업적 공급 업체에서 구할 수 있거나, 발표된 제조 방법(예를 들어, American Type Culture Collection의 카탈로그)에 따라 준비할 수 있다.

또 다른 측면에서, 본 발명은 본원에서 제공되는 개선된 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하며, 상기 폴리뉴클레오타이드는 숙주 세포에서 퓨린 뉴클레오사이드 포스포릴라제 효소의 발현을 위해 하나 이상의 제어 서열에 작동 가능하게 연결된다. 본 발명의 발현 벡터에 의해 코딩되는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 발현하는데 사용하기 위한 숙주 세포는 관련 기술 분야에 잘 알려져 있으며, 박테리아 세포, 예를 들어 이. 콜라이, 바실러스 메가테리움(Bacillus megaterium), 락토바실러스 케피르( Lactobacillus kefir), 스트렙토마이세스 및 살모넬라 티피무리움 세포; 진균 세포, 예를 들어, 효모 세포(예를 들어, 사카로마이세스 세레비지애 또는 피키아 파스토리스(ATCC 수탁 번호 201178)); 곤충 세포, 예를 들어 초파리 S2 및 스포도프테라 Sf9 세포; 동물 세포, 예를 들어 CHO, COS, BHK, 293 및 보우스 흑색종 세포; 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 상기 설명한 숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 관련 기술 분야에 잘 알려져 있다.

퓨린 뉴클레오사이드 포스포릴라제의 발현을 위한 폴리뉴클레오타이드는 관련 기술 분야에 공지된 다양한 방법에 의해 세포 내로 도입될 수 있다. 기술에는 전기천공, 바이올리스틱(biolistic) 입자 충격, 리포좀 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합이 포함된다. 폴리뉴클레오타이드를 세포 내로 도입하기 위한 다양한 방법이 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다.

일부 실시양태에서, 숙주 세포는 진핵 세포이다. 적합한 진핵 숙주 세포는 진균 세포, 조류 세포, 곤충 세포 및 식물 세포를 포함하지만, 이로 제한되지 않는다. 적합한 진균 숙주 세포는 아스코미코타(Ascomycota), 바시디오미코타(Basidiomycota), 듀테로미코타(Deuteromycota), 자이고미코타(Zygomycota), 펑가이 임페르펙티(Fungi imperfecti)를 포함하지만, 이로 제한되지 않는다. 일부 실시양태에서, 진균 숙주 세포는 효모 세포 및 사상형 진균 세포이다. 본 발명의 사상형 진균 숙주 세포는 아문(subdivision) 유미코티나(Eumycotina) 및 오오미코타(Oomycota)의 모든 사상형 형태를 포함한다. 사상형 진균은 키틴, 셀룰로스 및 기타 복잡한 폴리사카라이드로 이루어진 세포벽을 가진 영양 균사체를 특징으로 한다. 본 발명의 사상형 진균 숙주 세포는 형태학적으로 효모와 구별된다.

본 발명의 일부 실시양태에서, 사상형 숙주 세포는 아클리아(Achlya), 아크레모늄(Acremonium), 아스퍼질러스, 아우레오바시디움(Aureobasidium), 브저르칸데라(Bjerkandera), 세리포리옵시스(Ceriporiopsis), 세팔로스포리움(Cephalosporium), 크리소스포리움(Chrysosporium), 코클리오볼루스(Cochliobolus), 코리나스쿠스(Corynascus), 크로포넥트리아(Cryphonectria), 크립토코커스(Cryptococcus), 코프리누스(Coprinus), 콜리올루스(Coriolus), 디플로디아(Diplodia), 엔도티스(Endothis), 푸사리움(Fusarium), 지베렐라(Gibberella), 글리오클라디움(Gliocladium), 휴미콜라(Humicola), 하이포크레아(Hypocrea), 마이셀리오프토라(Myceliophthora), 무코르(Mucor), 뉴로스포라(Neurospora), 페니실리움(Penicillium), 포도스포라(Podospora), 플레비아(Phlebia), 피로마이세스(Piromyces), 피리쿨라리아(Pyricularia), 리조무코르(Rhizomucor), 리조푸스(Rhizopus), 쉬조필룸(Schizophyllum), 스키탈리디움(Scytalidium), 스포로트리쿰(Sporotrichum), 탈라로마이세스(Talaromyces), 써모아스쿠스(Thermoascus), 티엘라비아(Thielavia), 트라메테스(Trametes), 톨리포클라디움(Tolypocladium), 트리코데르마(Trichoderma), 베르티실리움(Verticillium), 및/또는 볼바리엘라(Volvariella), 및/또는 이들의 유성생식형, 또는 무성생식형 및 동의어, 기본명(basionym) 또는 분류학적 동등물을 포함하지만 이로 제한되지 않는 임의의 적합한 속 및 종의 숙주 세포이다.

본 발명의 일부 실시양태에서, 숙주 세포는 칸디다(Candida), 한세눌라(Hansenula), 사카로마이세스, 쉬조사카로마이세스(Schizosaccharomyces), 피키아, 클루이베로마이세스(Kluyveromyces), 또는 야로위아(Yarrowia) 종의 세포를 포함하지만 이로 제한되지 않는 효모 세포이다. 본 발명의 일부 실시양태에서, 효모 세포는 한세눌라 폴리모르파(Hansenula polymorpha), 사카로마이세스 세레비지애, 사카로마이세스 카를스페르겐시스(Saccharomyces carlsbergensis), 사카로마이세스 디아스타티쿠스(Saccharomyces diastaticus), 사카로마이세스 노르벤시스(Saccharomyces norbensis), 사카로마이세스 클루이베리(Saccharomyces kluyveri), 쉬조사카로마이세스 폼베(Schizosaccharomyces pombe), 피키아 파스토리스, 피키아 핀란디카(Pichia finlandica), 피키아 트레할로필라(Pichia trehalophila), 피키아코다마에(Pichia kodamae), 피키아 멤브라나에파시엔스(Pichia membranaefaciens), 피키아 오푼티아에(Pichia opuntiae), 피키아 써모톨레란스(Pichia thermotolerans), 피키아 살릭타리아(Pichia salictaria), 피키아 퀘르쿠움(Pichia quercuum), 피키아 피이페리(Pichia pijperi), 피키아 스티피티스(Pichia stipitis), 피키아 메타놀리카(Pichia methanolica), 피키아 안구스타(Pichia angusta), 클루이베로마이세스 락티스(Kluyveromyces lactis), 칸디다 알비칸스(Candida albicans), 또는 야로위아 리폴리티카(Yarrowia lipolytica)이다.

본 발명의 일부 실시양태에서, 숙주 세포는 조류 세포, 예를 들어 클라미도모나스(Chlamydomonas)(예를 들어, 씨. 레인하르드티이(C. reinhardtii)) 및 포르미디움(Phormidium)(피. 속의 종 ATCC29409)이다.

일부 다른 실시양태에서, 숙주 세포는 원핵 세포이다. 적합한 원핵 세포는 그람 양성, 그람 음성 및 그람 염색 가변성(Gram-variable) 박테리아 세포를 포함하지만, 이로 제한되지 않는다. 다음을 포함하지만 이로 제한되지 않는 임의의 적합한 박테리아 유기체가 본 발명에서 유용하다: 아그로박테리움(Agrobacterium), 알리시클로바실러스(Alicyclobacillus), 아나바에나(Anabaena), 아나시스티스(Anacystis), 아시네토박터(Acinetobacter), 아시도써무스(Acidothermus), 아르트로박터(Arthrobacter), 아조박터(Azobacter), 바실러스, 비피도박테리움(Bifidobacterium), 브레비박테리움(Brevibacterium), 부티리비브릭(Butyrivibrio), 부크네라(Buchnera), 캄페스트리스(Campestris), 캄플리오박터(Camplyobacter), 클로스트리디움(Clostridium), 코리네박테리움(Corynebacterium), 크로마티움(Chromatium), 코프로코쿠스(Coprococcus), 에셔리키아, 엔테로코쿠스(Enterococcus), 엔테로박터(Enterobacter), 에르위니아(Erwinia), 푸소박테리움(Fusobacterium), 파에칼리박테리움(Faecalibacterium), 프란시셀라(Francisella), 플라보박테리움(Flavobacterium), 게오바실러스(Geobacillus), 해모필루스(Haemophilus), 헬리코박터(Helicobacter), 클렙시엘라(Klebsiella), 락토바실러스(Lactobacillus), 락토코쿠스(Lactococcus), 일리오박터(Ilyobacter), 미크로코쿠스(Micrococcus), 미크로박테리움(Microbacterium), 메소리조븀(Mesorhizobium), 메틸로박테리움(Methylobacterium), 메틸로박테리움, 미코박테리움(Mycobacterium), 네이세리아(Neisseria), 판토에아(Pantoea), 슈도모나스(Pseudomonas), 프로클로로코쿠스(Prochlorococcus), 도도박터(Rhodobacter), 로도슈도모나스(Rhodopseudomonas), 로도슈도모나스, 로세부리아(Roseburia), 로도스피릴룸(Rhodospirillum), 로도코쿠스(Rhodococcus), 세네데스무스(Scenedesmus), 스트렙토마이세스, 스트렙토코쿠스(Streptococcus), 시네코쿠스(Synecoccus), 사카로모노스포라(Saccharomonospora), 스타필로코쿠스(Staphylococcus), 세라티아(Serratia), 살모넬라(Salmonella), 쉬겔라(Shigella), 써모아나에로박테리움(Thermoanaerobacterium), 트로페리마(Tropheryma), 툴라렌시스(Tularensis), 테메쿨라(Temecula), 써모시네코코쿠스(Thermosynechococcus), 써모코쿠스(Thermococcus), 우레아플라스마(Ureaplasma),잔토모나스(Xanthomonas), 자일렐라(Xylella), 예르시니아(Yersinia) 및 자이모모나스(Zymomonas). 일부 실시양태에서, 숙주 세포는 아그로박테리움, 아시네토박터, 아조박터, 바실러스, 비피도박테리움, 부크네라, 게오바실러스, 캄필로박터, 클로스트리듐, 코리네박테리움, 에셔리키아, 엔테로코쿠스, 에르위니아, 플라보박테리움, 락토바실러스, 락토코쿠스, 판토에아, 슈도모나스, 스타필로코쿠스, 살모넬라, 스트렙토코쿠스, 스트렙토마이세스, 또는 자이모모나스의 종이다. 일부 실시양태에서, 박테리아 숙주 균주는 인간에게 비병원성이다. 일부 실시양태에서, 박테리아 숙주 균주는 산업용 균주이다. 많은 박테리아 산업용 균주가 알려져 있고, 본 발명에 적합하다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 아그로박테리움 종(예를 들어, 에이. 라디오박터(A. radiobacter), 에이. 리조게네스(A. rhizogenes), 및 에이. 루비(A. rubi))이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 아르트로박터(Arthrobacter) 종(예를 들어, 에이. 아우레센스(A. aurescens), 에이. 시트레우스(A. citreus), 에이. 글로비포르미스(A. globiformis), 에이. 히드로카르보글루타미쿠스(A. hydrocarboglutamicus), 에이. 미소렌스(A. mysorens), 에이. 니코티아나에(A. nicotianae), 에이. 파라피네우스(A. paraffineus), 에이. 프로토포니아에(A. protophonniae), 에이. 로세오파르크피누스(A. roseoparqffinus), 에이. 술푸레우스(A. sulfureus), 및 에이. 우레아파시엔스(A. ureafaciens)이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 바실러스 종(예를 들어, 비. 투링겐시스(B. thuringensis), 비. 안트라시스(B. anthracis), 비. 메가테리움(B. megaterium), 비. 서브틸리스(B. subtilis), 비. 렌투스(B. lentus), 비. 시르쿨란스(B. circulans), 비. 푸밀루스(B. pumilus), 비. 라우투스(B. lautus), 비. 코아굴란스(B.coagulans), 비. 브레비스(B. brevis), 비. 피르무스(B. firmus), 비. 알카오피우스(B. alkaophius), 비. 리케니포르무스(B. licheniformis), 비. 클라우시이(B. clausii), 비. 스테아로써모필루스(B. stearothermophilus), 비. 할로두란스(B. halodurans), 및 비. 아밀로리퀘파시엔스(B. amyloliquefaciens)이다. 일부 실시양태에서, 숙주 세포는 비. 서브틸리스, 비. 푸밀루스, 비. 리케니포르무스, 비. 메가테리움, 비. 클라우시이, 비. 스테아로써모필루스 또는 비. 아밀로리퀘파시엔스를 포함하지만 이로 제한되지 않는 산업용 바실러스 균주이다. 일부 실시양태에서, 바실러스 숙주 세포는 비. 서브틸리스, 비. 리케니포르무스, 비. 메가테리움, 비. 스테아로써모필루스 및/또는 비. 아밀로리퀘파시엔스이다. 일부 실시양태에서, 박테리아 숙주 세포는 클로스트리디움 종(예를 들어, 씨. 아세토부틸리쿰(C. acetobutylicum), 씨. 테타니(C. tetani E88), 씨. 리투세부렌세(C. lituseburense), 씨. 사카로부틸리쿰(C. saccharobutylicum), 씨. 페르프링겐스(C. perfringens), 및 씨. 베이제링키이(C. beijerinckii)이다. 일부 실시양태에서, 박테리아 숙주 세포는 코리네박테리움 종(예를 들어, 씨. 글루타미쿰(C. glutamicum) 및 씨. 아세토아시도필룸(C. acetoacidophilum)이다. 일부 실시양태에서, 박테리아 숙주 세포는 에셔리키아 종(예를 들어, 이. 콜라이)이다. 일부 실시양태에서, 숙주 세포는 에셔리키아 콜라이 W3110이다. 일부 실시양태에서, 박테리아 숙주 세포는 에르위니아 종(예를 들어, 이. 우레도보라(E. uredovora), 이. 카로토보라(E. carotovora), 이. 아나나스(E. ananas), 이. 헤르비콜라(E. herbicola), 이. 푼크타타(E. punctata), 및 이. 테레우스(E. terreus)이다. 일부 실시양태에서, 박테리아 숙주 세포는 판토에아 종(예를 들어, 피. 시트레아(P. citrea) 및 피. 아글로메란스(P. agglomerans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 슈도모나스 종(예를 들어, 피. 푸티다(P. putida), 피. 아에루기노사(P. aeruginosa), 피. 메발로니이이(P. mevalonii), 및 피. 종 D-01 10)이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토코쿠스 종(예를 들어, 에스. 에퀴시밀레스(S. equisimiles), 에스. 피오게네스(S. pyogenes), 및 에스. 우베리스(S. uberis))이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토마이세스 종(예를 들어, 에스. 암보파시엔스(S. ambofaciens), 에스. 아크로모게네스(S. achromogenes), 에스. 아베르미틸리스(S. avermitilis), 에스. 코엘리콜로르(S. coelicolor), 에스. 아우레오파시엔스(S. aureofaciens), 에스. 아우레우스(S. aureus), 에스. 푼기시디쿠스(S. fungicidicus), 에스. 그리세우스(S. griseus), 및 에스. 리비단스(S. lividans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 자이모모나스 종(예를 들어, 지. 모빌리스(Z. mobilis), 및 지. 리폴리티카(Z. lipolytica))이다.

본 발명에서 유용한 많은 원핵 및 진핵 균주는 아메리칸 타입 컬쳐 컬렉션(ATCC), 도이체 잠룽 폰 미크로오르가니스멘 운트 젤쿨투렌 게엠베하(DSM: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), 센트랄뷰로 포르 쉼멜쿨투레스(CBS: Centraalbureau Voor Schimmelcultures) 및 애그리컬쳐럴 리써치 서비스 패턴트 컬쳐 컬렉션(Agricultural Research Service Patent Culture Collection), 노던 리져널 리써치 센터(NRRL: Northern Regional Research Center)와 같은 다수의 배양물 기탁 기관으로부터 공개적으로 쉽게 이용할 수 있다.

일부 실시양태에서, 숙주 세포는 단백질 분비, 단백질 안정성 및/또는 단백질의 발현 및/또는 분비에 바람직한 다른 특성을 개선하는 특징을 갖도록 유전적으로 변형된다. 유전자 변형은 유전공학 기술 및/또는 고전적인 미생물 기술(예를 들어, 화학적 또는 UV 돌연변이 유발 및 후속 선별)에 의해 달성될 수 있다. 실제로, 일부 실시양태에서, 재조합 변형 및 고전적인 선별 기술의 조합이 숙주 세포를 생산하기 위해 사용된다. 재조합 기술을 사용하여, 핵산 분자는 숙주 세포 내에서 및/또는 배양 배지에서 퓨린 뉴클레오사이드 포스포릴라제 변이체(들)의 수율을 증가시키는 방식으로 도입, 결실, 억제 또는 변형될 수 있다. 예를 들어, Alp1 기능의 녹아웃(knockout)은 프로테아제가 결핍된 세포를 생성하고, pyr5 기능의 녹아웃은 피리미딘 결핍 표현형을 가진 세포를 생성한다. 하나의 유전공학 방법에서, 상동성 재조합은 코딩된 단백질의 발현을 억제하기 위해 생체 내에서 유전자를 특이적으로 표적화함으로써 표적화된 유전자 변형을 유도하기 위해 사용된다. 대안적 접근법에서, siRNA, 안티센스 및/또는 리보자임 기술이 유전자 발현 억제에 유용하다. 단백질을 코딩하는 유전자의 전부 또는 일부의 결실 및 유전자 생성물의 발현 또는 활성을 파괴하기 위한 부위-특이적 돌연변이 유발을 포함하지만 이로 제한되지 않는 세포에서 단백질의 발현을 감소시키는 다양한 방법이 관련 기술 분야에 공지되어 있다(예를 들어, 그 전체가 본원에 참고로 포함된 문헌 [Chaveroche et al., Nucl. Acids Res., 28:22 e97 [2000]; Cho et al., Molec. Plant Microbe Interact., 19:7-15 [2006]; Maruyama and Kitamoto, Biotechnol Lett., 30:1811-1817 [2008]; Takahashi et al., Mol. Gen. Genom., 272: 344-352 [2004]; 및 You et al., Arch. Microbiol.,191:615-622 [2009] 참조). 무작위 돌연변이 유발, 이어서 원하는 돌연변이에 대한 스크리닝도 유용하다(예를 들어, 둘 모두 참고로 포함된 문헌 [Combier et al., FEMS Microbiol. Lett., 220:141-8 [2003]; 및 Firon et al., Eukary. Cell 2:247-55 [2003]] 참조)).

벡터 또는 DNA 구축물을 숙주 세포에 도입하는 것은 인산칼슘 형질감염, DEAE-덱스트란 매개 형질감염, PEG 매개 형질전환, 전기천공 또는 관련 기술 분야에 공지된 다른 통상적인 기술을 포함하지만 이로 제한되지 않는, 관련 기술 분야에 공지된 임의의 적합한 방법을 사용하여 달성될 수 있다. 일부 실시양태에서, 에셔리키아 콜라이 발현 벡터 pCK100900i(본 명세서에 참조로 포함된 미국 특허 제9,714,437호 참조)가 유용하다.

일부 실시양태에서, 본 발명의 조작된 숙주 세포(즉, "재조합 숙주 세포")는 프로모터를 활성화하거나, 형질전환체를 선별하거나, 퓨린 뉴클레오사이드 포스포릴라제 폴리뉴클레오타이드를 증폭하기 위해 적절하게 변형된 통상적인 영양 배지에서 배양된다. 배양 조건, 예를 들어 온도, pH 등은 발현을 위해 선택된 숙주 세포와 함께 이전에 사용된 것이며, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려져 있다. 언급한 바와 같이, 박테리아, 식물, 동물(특히 포유동물) 및 고세균 기원의 세포를 포함하는 많은 세포의 배양 및 생산에 대한 많은 표준 참고문헌 및 텍스트를 사용할 수 있다.

일부 실시양태에서, 본 발명의 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 발현하는 세포는 배치 또는 연속 발효 조건 하에서 성장된다. 고전적인 "배치 발효"는 폐쇄 시스템으로서, 배지의 조성은 발효 초기에 설정되고, 발효 동안 인공적으로 변경되지 않는다. 배치 시스템의 변형은 본 발명에서도 사용되는 "유가식 발효"이다. 이 변형에서, 기질은 발효가 진행됨에 따라 증분으로 첨가된다. 유가식 시스템은 이화대사물 억제가 세포의 대사를 억제할 가능성이 있고 배지에 제한된 양의 기질이 존재하는 것이 바람직한 경우에 유용하다. 배치 및 유가식 발효는 일반적이며, 관련 기술 분야에 잘 알려져 있다. "연속 발효"는 개방 시스템으로서, 규정된 발효 배지가 생물반응기에 연속적으로 첨가되고 등량의 조건화된 배지가 처리를 위해 동시에 제거된다. 일반적으로 연속 발효는 배양액을 세포를 주로 로그 단계 성장에서 일정한 고밀도로 유지한다. 연속 발효 시스템은 항정 상태의 성장 조건을 유지하기 위해 노력한다. 연속 발효 공정을 위한 영양소 및 성장 인자를 조절하는 방법 및 생성물 형성 속도를 최대화하는 기술은 산업 미생물학 분야에서 잘 알려져 있다.

본 발명의 일부 실시양태에서, 무세포 전사/번역 시스템이 변이체 퓨린 뉴클레오사이드 포스포릴라제(들)를 생산하기 위해 사용된다. 여러 시스템이 상업적으로 이용 가능하며, 그 방법은 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려져 있다.

본 발명은 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드 또는 이의 생물학적 활성 단편을 제조하는 방법을 제공한다. 일부 실시양태에서, 상기 방법은 서열 번호 2, 6, 126, 242, 684에 대한 적어도 약 70%(또는 적어도 약 75%, 적어도 약 80%, 적어도 약 85%, 적어도 약 90%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%)의 서열 동일성을 포함하고 본원에서 제공되는 바와 같은 적어도 하나의 돌연변이를 포함하는 아미노산 서열을 코딩하는 폴리뉴클레오타이드로 형질전환된 숙주 세포를 제공하는 단계, 숙주 세포가 코딩되는 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 발현하는 조건 하에서 형질전환된 숙주 세포를 배양 배지에서 배양하는 단계; 및 선택적으로, 발현된 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 회수 또는 단리하고/하거나, 발현된 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 함유하는 배양 배지를 회수 또는 단리하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 선택적으로, 코딩되는 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 발현한 후에 형질전환된 숙주 세포를 용해시키고, 선택적으로, 발현된 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 세포 용해물로부터 회수 및/또는 단리하는 것을 추가로 제공한다. 본 발명은 또한 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 생산에 적합한 조건 하에서 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드로 형질전환된 숙주 세포를 배양하고, 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 회수하는 것을 포함하는, 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 제조 방법을 추가로 제공한다. 전형적으로, 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 회수 또는 단리는 본원에서 설명되는 것을 포함하여 관련 기술 분야에 잘 알려진 단백질 회수 기술을 사용하여 숙주 세포 배양 배지, 숙주 세포 또는 둘 모두로부터 이루어진다. 일부 실시양태에서, 숙주 세포는 원심분리에 의해 수거되고, 물리적 또는 화학적 수단에 의해 파괴되고, 생성된 조질 추출물은 추가의 정제를 위해 보유된다. 단백질 발현에 사용되는 미생물 세포는 동결-해동 사이클, 초음파 처리, 기계적 파괴 및/또는 세포 용해제의 사용을 포함하지만 이로 제한되지 않는 임의의 편리한 방법뿐만 아니라, 관련 기술 분야에서 통상의 기술을 가진 자에게 잘 알려진 많은 다른 적합한 방법에 의해 파괴될 수 있다.

숙주 세포에서 발현되는 조작된 퓨린 뉴클레오사이드 포스포릴라제 효소는 특히 리소자임 처리, 초음파 처리, 여과, 염석, 초원심분리 및 크로마토그래피를 포함하는, 단백질 정제를 위해 관련 기술 분야에 공지된 임의의 하나 이상의 기술을 사용하여 세포 및/또는 배양 배지로부터 회수될 수 있다. 이. 콜라이와 같은 박테리아로부터 단백질을 용해하고 고효율로 추출하기에 적합한 용액은 상표명 CelLytic B™(Sigma-Aldrich)로 시판된다. 따라서, 일부 실시양태에서, 생성된 폴리펩타이드는 관련 기술 분야에 공지된 다수의 방법 중 임의의 것에 의해 회수/단리되고, 선택적으로 정제된다. 예를 들어, 일부 실시양태에서, 폴리펩타이드는 원심분리, 여과, 추출, 분무 건조, 증발, 크로마토그래피(예를 들어, 이온 교환, 친화도, 소수성 상호작용, 크로마토포커싱 및 크기 배제) 또는 침전을 포함하지만 이로 제한되지 않는 통상적인 절차에 의해 영양 배지로부터 단리된다. 일부 실시양태에서, 단백질 재폴딩 단계가 성숙 단백질의 구성을 완료할 때 필요에 따라 사용된다. 또한, 일부 실시양태에서, 고성능 액체 크로마토그래피(HPLC)가 최종 정제 단계에서 사용된다. 예를 들어, 일부 실시양태에서, 관련 기술 분야에 공지된 방법이 본 발명에서 유용하다(예를 들어, 둘 모두 본원에 참조로 포함된 문헌 [Parry et al., Biochem. J., 353:117 [2001]; 및 Hong et al., Appl. Microbiol. Biotechnol., 73:1331 [2007]] 참조). 실제로, 관련 기술 분야에 알려진 임의의 적합한 정제 방법이 본 발명에서 유용하다.

퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드의 단리를 위한 크로마토그래피 기술은 역상 크로마토그래피, 고성능 액체 크로마토그래피, 이온 교환 크로마토그래피, 겔 전기영동 및 친화도 크로마토그래피를 포함하지만 이로 제한되지 않는다. 특정 효소를 정제하기 위한 조건은 부분적으로는, 순 전하, 소수성, 친수성, 분자량, 분자 형태 등과 같은 인자에 따라 달라질 것이며, 이는 관련 기술 분야에서 통상의 기술을 가진 자에게 공지되어 있다.

일부 실시양태에서, 친화도 기술은 개선된 퓨린 뉴클레오사이드 포스포릴라제 효소를 단리하는데 유용하다. 친화도 크로마토그래피 정제를 위해, 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드에 특이적으로 결합하는 임의의 항체가 사용될 수 있다. 항체 생산을 위해, 토끼, 마우스, 래트 등을 포함하지만 이로 제한되지 않는 다양한 숙주 동물이 퓨린 뉴클레오사이드 포스포릴라제 주사에 의해 면역화될 수 있다. 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드는 측쇄 작용기 또는 측쇄 작용기에 부착된 링커에 의해 BSA와 같은 적합한 담체에 부착될 수 있다. 프로인트(Freund)(완전 및 불완전), 수산화알루미늄과 같은 미네랄 겔, 리솔레시틴과 같은 표면 활성 물질, 플루로닉 폴리올, 다가음이온, 펩타이드, 오일 에멀젼, 키홀 림펫 헤모시아닌, 디니트로페놀, 및 잠재적으로 유용한 인간 보조제, 예를 들어 BCG(바실러스 칼메테 구에린(Bacillus Calmette Guerin)) 및 코리네박테리움 피르붐(Corynebacterium parvum)을 포함하지만 이로 제한되지 않는 숙주 종에 따라 면역 반응을 증가시키기 위해 다양한 보조제가 사용될 수 있다.

일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 변이체는 효소를 발현하는 세포의 형태로, 조질 추출물로서, 또는 단리되거나 정제된 제제로서 제조되고 사용된다. 일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 변이체는 동결건조물, 분말 형태(예를 들어, 아세톤 분말)로 제조되거나, 효소 용액으로 제조된다. 일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 변이체는 실질적으로 순수한 제제의 형태로 존재한다.

일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드는 임의의 적합한 고체 기재에 부착된다. 고체 기재는 고체상, 표면 및/또는 막을 포함하지만, 이로 제한되지 않는다. 고체 지지체는 폴리스티렌, 폴리에틸렌, 폴리프로필렌, 폴리플루오로에틸렌, 폴리에틸렌옥시 및 폴리아크릴아미드뿐만 아니라, 이들의 공중합체 및 그라프트를 포함하지만, 이로 제한되지 않는다. 고체 지지체는 또한 무기물, 예를 들어 유리, 실리카, 제어된 기공 유리(CPG), 역상 실리카 또는 금 또는 백금과 같은 금속일 수 있다. 기재의 배열은 비드, 구체, 입자, 과립, 겔, 막 또는 표면의 형태일 수 있다. 표면은 평면형, 실질적으로 평면형 또는 비평면형일 수 있다. 고체 지지체는 다공성이거나 비다공성일 수 있으며, 팽윤 또는 비팽윤 특성을 가질 수 있다. 고체 지지체는 웰, 침하부 또는 다른 컨테이너, 용기, 특징체(feature) 또는 구획(location)의 형태로 구성될 수 있다. 시약의 로봇에 의한 전달을 위해 또는 검출 방법 및/또는 기기에 의해 주소 지정 가능한 다양한 위치의 어레이에 복수의 지지체를 구성할 수 있다.

일부 실시양태에서, 퓨린 뉴클레오사이드 포스포릴라제 변이체를 정제하기 위해 면역학적 방법이 사용된다. 한 접근법에서, 통상적인 방법을 사용하여 야생형 또는 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드에 대해(예를 들어, 서열 번호 2, 6, 126, 242, 및/또는 684 중 어느 하나를 포함하는 폴리펩타이드, 및/또는 이의 면역원성 단편에 대해) 생성된 항체를 비드에 고정시키고, 변이체 퓨린 뉴클레오사이드 포스포릴라제가 결합되어 침전되는 조건 하에서 세포 배양 배지와 혼합한다. 관련 접근 방식에서, 면역 크로마토그래피가 유용하다.

일부 실시양태에서, 변이체 퓨린 뉴클레오사이드 포스포릴라제는 비-효소 부분을 포함하는 융합 단백질로서 발현된다. 일부 실시양태에서, 변이체 퓨린 뉴클레오사이드 포스포릴라제 서열은 정제 촉진 도메인에 융합된다. 본원에서 사용되는 바와 같이, 용어 "정제 촉진 도메인"은 그것이 융합되는 폴리펩타이드의 정제를 매개하는 도메인을 지칭한다. 적합한 정제 도메인은 금속 킬레이팅 펩타이드, 고정된 금속 상에서의 정제를 허용하는 히스티딘-트립토판 모듈, 글루타티온(예를 들어, GST)에 결합하는 서열, 헤마글루티닌(HA) 태그(인플루엔자 헤마글루티닌 단백질로부터 유래된 에피토프에 상응함; 예를 들어, 문헌 [Wilson et al., Cell 37:767 [1984]] 참조), 말토오스 결합 단백질 서열, FLAGS 연장/친화도 정제 시스템(예를 들어, Immunex Corp에서 입수 가능한 시스템)에서 사용되는 FLAG 에피토프 등을 포함하지만, 이로 제한되지 않는다. 본원에서 설명되는 조성물 및 방법에서 사용하기 위해 고려되는 하나의 발현 벡터는 엔테로키나제 절단 부위에 의해 분리된 폴리히스티딘 영역에 융합된 본 발명의 폴리펩타이드를 포함하는 융합 단백질의 발현을 제공한다. 히스티딘 잔기는 IMIAC(고정된 금속 이온 친화도 크로마토그래피; 문헌 [Porath el al., Prot. Exp. Purif., 3:263-281 [1992]] 참조)에서 정제를 촉진하는 반면, 엔테로키나제 절단 부위는 융합 단백질로부터 변이체 퓨린 뉴클레오사이드 포스포릴라제 폴리펩타이드를 분리하기 위한 수단을 제공한다. pGEX 벡터(Promega)는 또한 글루타티온 S-트랜스퍼라제(GST)와의 융합 단백질로서 외래 폴리펩타이드를 발현하기 위해 사용될 수 있다. 일반적으로, 이러한 융합 단백질은 가용성이며, 리간드-아가로스 비드(예를 들어, GST-융합의 경우 글루타티온-아가로스)에 흡착한 다음, 유리 리간드의 존재 하에 용리함으로써 용해된 세포로부터 쉽게 정제될 수 있다.

따라서, 또 다른 측면에서, 본 발명은 조작된 효소 폴리펩타이드를 생산하는 방법을 제공하며, 상기 방법은 폴리펩타이드의 발현에 적합한 조건 하에서 조작된 효소 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 발현할 수 있는 숙주 세포를 배양하는 단계를 포함한다. 일부 실시양태에서, 상기 방법은 본원에서 설명되는 바와 같이 효소 폴리펩타이드를 단리 및/또는 정제하는 단계를 추가로 포함한다.

숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 관련 기술 분야에 잘 알려져 있다. 효소 폴리펩타이드의 발현을 위한 폴리뉴클레오타이드를 세포 내로 도입하는 임의의 적합한 방법이 본 발명에서 유용할 것으로 고려된다. 적합한 기술은 전기천공, 바이올리스틱 입자 충격, 리포좀 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합을 포함하지만, 이로 제한되지 않는다.

본 발명의 다양한 특징 및 실시양태는 하기 대표적인 실시예에서 예시되고, 이것은 제한하는 것이 아니라 예시적인 것으로 의도된다.

실험

달성된 실험 및 결과를 포함하는 하기 실시예는 단지 예시 목적으로만 제공되는 것으로서, 본 발명을 제한하는 것으로 해석되지 않아야 한다. 실제로, 아래에서 설명되는 많은 시약 및 장비에 적합한 다양한 공급처가 존재한다. 본 발명은 임의의 시약 또는 장비 품목에 대한 임의의 특정 공급처로 제한되도록 의도되지 않는다. 일부 실시양태에서, C-말단의 히스티딘 태그(즉, 6개의 히스티딘 잔기)가 폴리펩타이드 서열에 포함된다. 본원과 함께 제출된 서열 목록은 상기 히스티딘 태그가 없는 폴리펩타이드 서열을 포함한다.

아래에서 개시되는 실험에서, 다음 약어가 적용된다: M (몰); mM (밀리몰), uM 및 μM (마이크로몰); nM (나노몰); mol (몰); gm 및 g(그램); mg (밀리그램); ug 및 μg (마이크로그램); L 및 l (리터); ml 및 mL (밀리리터); cm (센티미터); mm (밀리미터); um 및 μm (마이크로미터); sec. (초); min(s) (분); h(s) 및 hr(s) (시간); U (단위); MW (분자량); rpm (분당 회전); psi 및 PSI (평방인치당 파운드); ℃(섭씨 온도); RT 및 rt(실온); CV(변동 계수); CAM 및 cam (클로람페니콜); PMBS (폴리믹신 B 설페이트); IPTG (이소프로필 β-D-1-티오갈락토피라노사이드); LB (용원성 브로쓰); TB (테리픽 브로쓰); SFP (진탕 플라스크 분말); CDS (코딩 서열); DNA (데옥시리보핵산); RNA (리보핵산); nt (뉴클레오타이드; 폴리뉴클레오타이드); aa (아미노산; 폴리펩타이드); 이. 콜라이 W3110(일반적으로 사용되는 실험용 이. 콜라이 균주, Coli Genetic Stock Center [CGSC] (미국 코네티컷주 뉴 헤이븐 소재)로부터 이용가능함); HTP (고처리량); HPLC (고압 액체 크로마토그래피); HPLC-UV (HPLC- 자외선 가시광선 검출기); 1H NMR (양성자 핵자기 공명 분광법); FIOPC (양성 대조군 대비 개선 배수); 시그마-알드리치 (Sigma-Aldrich, 미국 미주리주 세인트루이스 소재); 디프코 (Difco Laboratories, BD Diagnostic Systems, 미국 미시건주 디트로이트 소재); 마이크로플루이딕스 (Microfluidics, 미국 매사추세츠주 웨스트우드 소재); 라이프 테크놀로지스 (Life Technologies, Fisher Scientific의 자회사, 미국 매사추세츠주 월담 소재); 암레스코 (Amresco, LLC, 미국 오하이오주 솔론 소재); 카르보신트 (Carbosynth, Ltd., 영국 버크셔 소재); 바리안 (Varian Medical Systems, 미국 캘리포니아주 팔로 알토 소재); 애질런트 (Agilent Technologies, Inc., 미국 캘리포니아주 산타 클라라 소재); 인포르스 (Infors USA Inc., 미국 메릴랜드주 아나폴리스 정션 소재); 및 써모트론 (Thermotron, Inc., 미국 미시건주 홀란드 소재).

실시예 1

습식 세포 펠렛을 포함하는 HTP PNP의 제조

본 발명의 변이체를 생산하기 위해 사용된 PNP(서열 번호 2) 효소의 모 유전자는 이. 콜라이 게놈으로부터 수득하고, pCK110900 벡터에 클로닝하였다. W3110 이. 콜라이 세포를 PNP 코딩 유전자를 포함하는 각각의 플라스미드로 형질전환하고, 1% 글루코스 및 30 ㎍/ml 클로람페니콜(CAM)을 포함하는 LB 한천 플레이트에 플레이팅하고, 37℃에서 밤새 성장시켰다. 모노클로날 콜로니를 골라서 1% 글루코스 및 30 ㎍/mL 클로람페니콜을 함유하는 180 ㎕ LB에 접종하고, 96웰의 얕은 웰 미량역가 플레이트의 웰에 위치시켰다. 플레이트를 O₂ 투과성 밀봉재로 밀봉하고, 배양물을 30℃, 200 rpm 및 85% 습도에서 밤새 성장시켰다. 그런 다음, 10 ㎕의 각각의 세포 배양물을 390 ㎕ TB 및 30 ㎍/mL CAM을 포함하는 96웰의 깊은 웰 플레이트의 웰로 옮겼다. 깊은 웰 플레이트를 O₂ 투과성 밀봉재로 밀봉하고, OD₆₀₀ 0.6-0.8에 도달할 때까지 30℃, 250 rpm 및 85% 습도에서 인큐베이팅하였다. 그런 다음, 이소프로필 티오글리코사이드(IPTG)를 1 mM의 최종 농도로 첨가하여 세포 배양을 유도하고, 250 rpm으로 진탕하면서 30℃에서 밤새 인큐베이팅하였다. 이어서, 세포를 10분 동안 4,000 rpm에서의 원심분리를 사용하여 세포를 펠렛화하였다. 상청액을 버리고, 펠렛을 용해 전에 -80℃에서 냉동시켰다.

실시예 2

HTP PNP 함유 세포 용해물의 제조

실시예 1에 기재된 바와 같이 제조된 동결 펠렛을 100 mM 트리에탄올아민 완충제, pH 7.5, 1 mg/mL 리소자임, 0.5 mg/mL PMBS 및 5 mM MnCl₂를 함유하는 200 ㎕ 용해 완충제로 용해시켰다. 용해 혼합물을 실온에서 2시간 동안 진탕하였다. 그런 다음, 플레이트를 4000 rpm 및 4℃에서 15분 동안 원심분리하였다. 이어서, 상청액은 활성 수준을 결정하기 위해 정화된 용해물로서 생촉매 반응에 사용되었다.

실시예 3

진탕 플라스크(SF) 배양물로부터 동결건조된 용해물의 제조

1% 글루코스 및 30 ㎍/ml CAM이 있는 LB 한천 플레이트로부터 원하는 유전자를 포함하고 37℃에서 밤새 인큐베이팅된 단일 콜로니를 1% 글루코스 및 30 ㎍/ml CAM이 있는 6 ml의 LB로 옮겼다. 배양물을 30℃, 250 rpm에서 18시간 동안 성장시키고, 30 ㎍/ml CAM을 포함하는 250 ml의 TB에 약 1:50으로 약 0.05의 최종 OD₆₀₀까지 계대배양하였다. 30℃, 250 rpm에서 약 195분 동안 0.6-0.8의 OD₆₀₀까지 계대배양물을 성장시키고, 1 mM IPTG로 유도하였다. 그런 다음, 계대배양물을 30℃, 250 rpm에서 20시간 동안 성장시켰다. 계대 배양물은 4000 rpm에서 20분 동안 원심분리하였다. 상청액을 버리고, 펠렛을 5 mM MnCl₂와 함께 35 ml의 25 mM 트리에탄올아민 완충제(pH 7.5)에 재현탁시켰다. 세포를 18,000 psi에서 마이크로플루이다이저(Microfluidizer)® 프로세서 시스템(Microfluidics)을 사용하여 용해시켰다. 용해물을 펠렛화하고(10,000 rpm x 60분), 상청액을 동결하고, 동결 건조하여 진탕 플레이크(SF) 효소 분말을 생성하였다.

실시예 4

화합물 1의 생산을 위한 개선된 퓨린 뉴클레오사이드 포스포릴라제 변이체

상기 실험을 위해, 서열 번호 2가 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 50 mM TEoA, 5 mM MnCl₂(pH 7.5)에 8배 희석하였다. 각각 100 ㎕의 반응은 pH 7.5에서 50%(v/v) 희석된 용해물, 30 mM 화합물 4, 36 mM 화합물 2, 5 g/L PPM(서열 번호 1004), 100 mM TEoA 완충제 및 5.0 mM MnCl₂를 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 열 밀봉하고, 45℃에서 인큐베이팅하고, 인포르스 써모트론(Infors Thermotron)^® 진탕기에서 500 rpm으로 밤새 교반하였다. 플레이트를 제거하고, 1 부피의 1:1의 DMSO:1 M KOH를 첨가하여 급냉시키고, 화합물이 용해될 때까지 잘 혼합한 다음, 분석 전에 25:75 v:v의 아세토니트릴:0.1 M TEoA(pH 7)로 10배 희석하였다.

서열 번호 2와 비교한 활성은 서열 번호 2에 의해 생성된 전환 비율과 비교하여 변이체 효소에 의해 형성된 생성물의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 HPLC 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 5

화합물 10의 생산을 위한 개선된 퓨린 뉴클레오사이드 포스포릴라제 변이체

분석 완건성(analytical robustness)을 개선하기 위해 대안적인 염기 교환 고처리량 스크리닝 방법이 개발되었다. 스크리닝 조건에 500 mM 브롬화칼륨을 포함시킴으로써, PNP 변이체도 브롬화칼륨 내성의 개선을 위해 스크리닝되었다. 상기 실험을 위해, 서열 번호 6이 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 200 ㎕ 대신에 400 ㎕의 용해 완충제 및 추가의 400 ㎕의 용해 완충제를 사용하여 실시예 2에 기술된 바와 같이 생성되었다.

각각 100 ㎕의 반응은 pH 7.5에서 100 mM TEoA 완충제 내의 5 ㎕의 용해물, 13 mM 화합물 (8), 15 mM 화합물 (9), 500 mM 브롬화칼륨 및 5 mM 인산암모늄을 사용하여 96웰 깊은 웰 미량역가 플레이트(2 mL 부피)에서 수행하였다. 플레이트를 열 밀봉하고, 40℃에서 인큐베이팅하고, 인포르스 써모트론^® 진탕기에서 600 rpm으로 밤새 교반하였다. 플레이트를 제거하고, 1 부피의 아세토니트릴을 첨가하여 급냉시키고, 화합물이 용해될 때까지 잘 혼합한 다음, pH 7.5의 800 ㎕의 100 mM TEoA 완충제를 첨가하여 5배 희석하였다. 40 ㎕의 희석된 급냉 샘플을 pH 7.5의 160 ㎕의 100 mM TEoA 완충제로 미리 채워진 96웰 밀리포어(Millipore) 필터 플레이트(0.45 ㎛ 기공 크기)로 옮기고, 혼합하고, 4℃에서 5분 동안 4000 rpm에서 원심분리한 후, HPLC에 의해 용리액을 분석하였다.

서열 번호 6과 비교한 활성은 서열 번호 6에 의해 생성된 전환 비율과 비교하여 변이체 효소에 의해 형성된 화합물 10의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 표 9.3에 기재된 바와 같이 HPLC 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 6

분석 완건성을 개선하기 위해 대안적인 염기 교환 고처리량 스크리닝 방법이 개발되었다. 스크리닝 조건에 500 mM 브롬화칼륨을 포함시킴으로써, PNP 변이체도 브롬화칼륨 내성의 개선을 위해 스크리닝되었다. 상기 실험을 위해, 서열 번호 6이 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 200 ㎕의 용해 완충제 대신에 400 ㎕의 용해 완충제를 사용하여 실시예 2에 기술된 바와 같이 생성되었다.

서열 번호 126과 비교한 활성은 서열 번호 126에 의해 생성된 전환 비율과 비교하여 변이체 효소에 의해 형성된 화합물 10의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 표 9.3에 기재된 바와 같이 HPLC 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 7

상기 실험을 위해, 서열 번호 242가 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 200 ㎕의 용해 완충제 대신에 400 ㎕의 용해 완충제를 사용하여 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 100 mM TEoA, 5 mM MnCl₂(pH 7.5)에 128배 희석하였다. 각각 100 ㎕의 반응은 pH 7.5에서 20 ㎕의 희석된 용해물, 98 mM 화합물 4, 196 mM 화합물 2, 10 g/L PPM 발효 분말(서열 번호 1006), 0.25 g/L 수크로스 포스포릴라제 SUP001(EC 2.4.1.7, 알로스카르도비아 옴니콜렌스(Alloscardovia omnicolens) SP154, GenBank 수탁 번호 WP_021617468.1), 196 mM 수크로스, 100 mM 황산칼륨, 100 mM TEoA 완충제 및 5.0 mM MnCl₂를 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 열 밀봉하고, 40℃에서 인큐베이팅하고, 인포르스 써모트론^® 진탕기에서 800 rpm으로 밤새 교반하였다. 플레이트를 제거하고, 200 ㎕의 1:1의 DMSO:1 M KOH를 첨가하여 급냉시키고, 화합물이 용해될 때까지 잘 혼합하였다. 10 ㎕의 희석된 급냉 샘플을 pH 7.5의 100 mM TEoA 완충제:아세토니트릴의 75:25 혼합물 190 ㎕로 미리 채워진 96웰 밀리포어 필터 플레이트(0.45 ㎛ 기공 크기)로 옮기고, 혼합하고, 4℃에서 5분 동안 4000 rpm에서 원심분리한 후, HPLC에 의해 용리액을 분석하였다.

서열 번호 242와 비교한 활성은 서열 번호 242에 의해 생성된 전환 비율과 비교하여 변이체 효소에 의해 형성된 화합물 1의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 표 9.2에 기재된 바와 같이 HPLC 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 8

상기 실험을 위해, 서열 번호 684가 모 효소로서 선택되었다. 잘 확립된 기술(예를 들어, 포화 돌연변이 유발 및 이전에 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자의 라이브러리를 생성하였다. 각각의 유전자에 의해 코딩된 폴리펩타이드는 실시예 1에 기술된 바와 같이 HTP에서 생산되었고, 정화된 용해물은 200 ㎕의 용해 완충제 대신에 400 ㎕의 용해 완충제를 사용하여 실시예 2에 기술된 바와 같이 생성되었다.

각각의 효소에 대해, 정화된 세포 용해물을 100 mM TEoA, 5 mM MnCl₂(pH 7.5)에 32배 희석하였다. 각각 100 ㎕의 반응은 pH 7.5에서 20 ㎕의 희석된 용해물, 98 mM 화합물 4, 196 mM 화합물 2, 10 g/L PPM46 발효 분말(서열 번호 514), 0.25 g/L 수크로스 포스포릴라제 SUP001, 196 mM 수크로스, 100 mM 황산칼륨, 100 mM TEoA 완충제 및 5.0 mM MnCl₂를 사용하여 96웰 얕은 웰 미량역가 플레이트에서 수행하였다. 플레이트를 열 밀봉하고, 40℃에서 인큐베이팅하고, 인포르스 써모트론^® 진탕기에서 800 rpm으로 밤새 교반하였다. 플레이트를 제거하고, 300 ㎕의 1:1의 DMSO:1 M KOH를 첨가하여 급냉시키고, 화합물이 용해될 때까지 잘 혼합하였다. 10 ㎕의 희석된 급냉 샘플을 pH 7.5의 100 mM TEoA 완충제:아세토니트릴의 75:25 혼합물 190 ㎕로 미리 채워진 96웰 밀리포어 필터 플레이트(0.45 ㎛ 기공 크기)로 옮기고, 혼합하고, 4℃에서 5분 동안 4000 rpm에서 원심분리한 후, HPLC에 의해 용리액을 분석하였다.

서열 번호 684와 비교한 활성은 서열 번호 684에 의해 생성된 전환 비율과 비교하여 변이체 효소에 의해 형성된 화합물 1의 전환 비율로서 계산되었다. 전환 비율은 생성물 피크의 면적을 표 9.2에 기재된 바와 같이 HPLC 분석에 의해 결정된 생성물 피크 및 기질의 면적의 총합으로 나눔으로써 정량하였다.

실시예 9

분석 방법

본 실시예는 상기 실시예들에서 제공된 데이터를 수집하기 위해 사용되는 방법을 제공한다. 실시예 4에 기재된 바와 같이 수득된 데이터는 표 9.1의 분석 방법을 사용하여 수집되었다. 실시예 6에 기재된 바와 같이 수득된 데이터는 표 9.2의 분석 방법을 사용하여 수집되었다. 실시예 5에 기재된 바와 같이 수득된 데이터는 표 9.3의 분석 방법을 사용하여 수집되었다. 본 실시예에서 제공되는 방법은 본 발명을 사용하여 생산된 변이체를 분석하는 데 유용하다. 그러나, 다른 적절한 방법이 관련 기술 분야에서 통상의 기술을 가진자에게 공지되어 있으므로, 본 발명이 본원에 기재된 방법으로 제한되는 것으로 의도되지 않는다.

본 출원에서 언급되는 모든 간행물, 특허, 특허 출원 및 기타 문서는 마치 각각의 개별 간행물, 특허, 특허 출원 또는 기타 문서가 모든 목적을 위해 참조로 통합된다고 개별적으로 표시되는 것과 동일한 정도로 모든 목적을 위해 그 전체가 본원에 참조로 통합된다.

다양한 특정 실시양태가 예시되고 설명되었지만, 본 발명(들)의 사상 및 범위를 벗어나지 않으면서 다양한 변경이 이루어질 수 있음을 이해할 것이다.

SEQUENCE LISTING <110> CODEXIS, INC. <120> ENGINEERED PURINE NUCLEOSIDE PHOSPHORYLASE VARIANT ENZYMES <130> CX2-172WO1 <140> <141> 2019-07-02 <150> 62/695,507 <151> 2018-07-09 <150> 62/822,263 <151> 2019-03-22 <160> 1006 <210> 1 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Codon optimized Purine Nucleoside Phosphorylase from E. Coli <400> 1 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 2 <211> 239 <212> PRT <213> Escherichia coli <220> <223> Purine Nucleoside Phosphorylase from E. Coli <400> 2 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 3 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Phosphopentomutase from Escherichia coli <400> 3 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 4 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> Phosphopentomutase from Escherichia coli <400> 4 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 5 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 5 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 6 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 6 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 7 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 7 atggctaccc ctcacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 8 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 8 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 9 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 9 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccgcgtaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 10 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 10 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ala Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 11 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 11 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccacgtaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 12 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 12 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Thr Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 13 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 13 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgtca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgctg tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 14 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 14 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Ser Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Leu Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 15 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 15 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtacgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 16 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 16 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Thr Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 17 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 17 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcacggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 18 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 18 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Thr Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 19 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 19 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcttgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 20 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 20 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Leu Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 21 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 21 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 22 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 22 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 23 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 23 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccgtttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 24 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 24 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Val Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 25 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 25 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtag ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 26 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 26 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ala Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 27 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 27 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtccgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 28 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 28 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Pro Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 29 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 29 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgaagtgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 30 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 30 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Lys Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 31 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 31 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtagtggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 32 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 32 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ser Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 33 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 33 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacgcgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 34 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 34 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ala Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 35 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 35 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 36 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 36 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 37 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 37 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttcagcgc ggacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 38 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 38 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 39 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 39 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca cctcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 40 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 40 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 41 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 41 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 taccggccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 42 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 42 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Arg Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 43 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 43 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccggggttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 44 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 44 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Gly Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 45 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 45 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccactg tcgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 46 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 46 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Cys Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 47 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 47 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttcagcgc ggacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 48 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 48 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 49 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 49 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcgttct cgccgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 50 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 50 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Val 165 170 175 Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 51 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 51 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcgttct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 52 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 52 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Val 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 53 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 53 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc atgaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 54 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 54 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Met Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 55 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 55 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 56 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 56 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 57 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 57 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg tgggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 58 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 58 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Val Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 59 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 59 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaacttttc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcactatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 60 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 60 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Thr Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 61 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 61 atgaataccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 62 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 62 Met Asn Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 63 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 63 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 64 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 64 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 65 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 65 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccctttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 66 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 66 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Leu Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 67 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 67 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 68 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 68 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 69 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 69 atggctaccc cacacattaa tgcagaaatg ggcaagttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcca cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 70 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 70 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Lys Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile His Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 71 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 71 atggctaccc cacacattaa tgcagaaatg ggctcgttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 72 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 72 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ser Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 73 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 73 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 74 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 74 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 75 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 75 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg ctggaaaaat acggcgttct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600 tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 76 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 76 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Leu Glu Lys Tyr Gly Val 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 77 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 77 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 ttggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 78 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 78 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Leu Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 79 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 79 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 80 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 80 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 81 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 81 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg agggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 82 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 82 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Glu Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 83 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 83 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccagggttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 84 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 84 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 85 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 85 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gctggatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 86 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 86 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Trp Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 87 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 87 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcttgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 88 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 88 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 89 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 89 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcgacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 90 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 90 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 91 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 91 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatgtaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctaggcga taaagag 717 <210> 92 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 92 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Met Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 93 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 93 atgtctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 94 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 94 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 95 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 95 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccgtggttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 96 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 96 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Val Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 97 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 97 atggctaccc cacacattaa tgcagaaatg ggctttttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 98 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 98 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Phe Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 99 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 99 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 cgggacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 100 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 100 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Arg Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 101 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 101 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcgttgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 102 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 102 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Val Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 103 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 103 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acgtgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 104 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 104 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 105 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 105 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 106 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 106 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 107 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 107 atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 108 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 108 Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 109 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 109 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 110 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 110 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 111 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 111 atggctaccc cacacattaa tgcagaaatg ggcctgttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 112 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 112 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Leu Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 113 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 113 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 114 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 114 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 115 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 115 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 116 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 116 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 117 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 117 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcat gagcagacca gctcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 118 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 118 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Ser Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 119 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 119 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acccagaaat gttcgacgtg atggaaaact acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca gcgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 120 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 120 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Asn Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Ser Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 121 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 121 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 122 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 122 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 123 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 123 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgttattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 124 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 124 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 125 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 125 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 126 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 126 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 127 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 127 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg cgggtatcca cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 128 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 128 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile His Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 129 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 129 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300 ctgcgcgacg tcctgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 130 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 130 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 131 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 131 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 132 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 132 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 133 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 133 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat cggaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 134 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 134 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 135 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 135 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 136 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 136 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 137 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 137 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggtattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 138 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 138 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 139 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 139 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 140 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 140 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 141 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 141 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg atggcgaaat gttcgacgtg atggaaaaat acgttattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 142 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 142 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 143 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 143 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 144 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 144 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 145 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 145 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 146 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 146 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 147 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 147 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggtattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 148 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 148 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 149 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 149 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 150 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 150 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 151 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 151 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 152 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 152 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 153 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 153 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 154 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 154 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 155 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 155 atgagcaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 156 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 156 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 157 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 157 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 158 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 158 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 159 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 159 atggctaccc cacacattaa tgcagaaatg ggcgacttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacacgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggcgatc 600 tgcacggtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 160 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 160 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 161 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 161 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggcgatc 600 tgcacggtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 162 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 162 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 163 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 163 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 164 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 164 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 165 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 165 atggctaccc cacacattaa tgcagaaatg ggcgacttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt cccgtgttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 166 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 166 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 167 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 167 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 168 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 168 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 169 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 169 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 170 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 170 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 171 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 171 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 172 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 172 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 173 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 173 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtag cggaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 174 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 174 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ala Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 175 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 175 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 176 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 176 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 177 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 177 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 178 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 178 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 179 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 179 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 180 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 180 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 181 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 181 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tataggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgttgaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 182 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 182 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ile Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 183 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 183 atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 184 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 184 Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 185 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 185 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcctgatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 186 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 186 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 187 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 187 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 188 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 188 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 189 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 189 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg atggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 190 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 190 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 191 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 191 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 192 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 192 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 193 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 193 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc cgaagaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca agggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaggaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 194 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 194 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Glu Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 195 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 195 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgttgaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 196 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 196 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 197 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 197 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtgg aaaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 198 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 198 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Glu Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 199 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 199 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgcgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttacg atggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga aaaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 200 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 200 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Asp Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 201 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 201 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccagggttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 202 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 202 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 203 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 203 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gctggatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 204 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 204 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Trp Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 205 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 205 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcttgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 206 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 206 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 207 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 207 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcgacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 208 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 208 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 209 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 209 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatgtaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctaggcga taaagag 717 <210> 210 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 210 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Met Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 211 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 211 atgtctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 212 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 212 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 213 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 213 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccgtggttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 214 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 214 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Val Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 215 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 215 atggctaccc cacacattaa tgcagaaatg ggctttttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 216 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 216 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Phe Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 217 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 217 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 cgggacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 218 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 218 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Arg Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 219 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 219 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcgttgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 220 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 220 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Val Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 221 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 221 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acgtgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 222 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 222 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 223 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 223 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacgacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 224 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 224 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 225 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 225 atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 226 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 226 Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 227 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 227 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 228 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 228 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 229 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 229 atggctaccc cacacattaa tgcagaaatg ggcctgttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 230 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 230 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Leu Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 231 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 231 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 232 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 232 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 233 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 233 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 234 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 234 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 235 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 235 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcat gagcagacca gctcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 236 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 236 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Ser Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 237 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 237 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tacagcccgg acccagaaat gttcgacgtg atggaaaact acggcattct cggcgtggaa 540 atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca gcgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 238 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 238 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Asn Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Ser Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 239 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 239 atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 240 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 240 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 241 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 241 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 242 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 242 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 243 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 243 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 244 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 244 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 245 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 245 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 246 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 246 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 247 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 247 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 248 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 248 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 249 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 249 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 250 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 250 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 251 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 251 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 252 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 252 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 253 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 253 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 254 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 254 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 255 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 255 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 256 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 256 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 257 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 257 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 258 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 258 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 259 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 259 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtattc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 260 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 260 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Ile Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 261 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 261 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 262 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 262 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 263 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 263 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggttatc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 264 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 264 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Tyr Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 265 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 265 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 266 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 266 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 267 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 267 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 268 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 268 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 269 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 269 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 270 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 270 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 271 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 271 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 272 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 272 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 273 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 273 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 274 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 274 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 275 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 275 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtacgc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 276 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 276 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Thr Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 277 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 277 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 278 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 278 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 279 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 279 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 280 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 280 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 281 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 281 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 282 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 282 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 283 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 283 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 284 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 284 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 285 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 285 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 286 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 286 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 287 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 287 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 288 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 288 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 289 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 289 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 290 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 290 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 291 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 291 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 292 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 292 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 293 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 293 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 294 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 294 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 295 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 295 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 296 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 296 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 297 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 297 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctggga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 298 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 298 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Trp Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 299 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 299 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 300 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 300 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 301 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 301 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 302 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 302 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 303 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 303 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 304 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 304 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 305 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 305 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 306 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 306 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 307 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 307 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 308 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 308 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 309 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 309 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 310 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 310 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 311 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 311 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtcctc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 312 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 312 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Pro Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 313 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 313 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 314 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 314 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 315 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 315 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 316 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 316 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 317 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 317 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 318 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 318 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 319 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 319 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 320 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 320 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 321 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 321 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtcgtc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 322 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 322 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Arg Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 323 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 323 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggttgtc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 324 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 324 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Cys Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 325 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 325 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gtttgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 326 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 326 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 327 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 327 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgcaggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 328 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 328 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 329 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 329 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 330 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 330 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 331 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 331 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccctgga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 332 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 332 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Leu Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 333 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 333 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 334 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 334 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 335 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 335 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 336 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 336 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 337 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 337 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 338 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 338 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 339 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 339 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 340 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 340 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 341 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 341 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct cgtttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 342 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 342 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Arg Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 343 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 343 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 344 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 344 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 345 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 345 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 346 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 346 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 347 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 347 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct atgttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 348 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 348 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Met Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 349 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 349 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 350 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 350 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 351 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 351 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatccct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 352 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 352 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Pro Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 353 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 353 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gcatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 354 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 354 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys His Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 355 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 355 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggttctc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 356 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 356 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Ser Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 357 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 357 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctatga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 358 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 358 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Tyr Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 359 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 359 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtttgc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 360 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 360 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Leu Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 361 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 361 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 362 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 362 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 363 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 363 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 364 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 364 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 365 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 365 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 366 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 366 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 367 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 367 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatggct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 368 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 368 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Met Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 369 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 369 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 370 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 370 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 371 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 371 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgattaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 372 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 372 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Ile Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 373 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 373 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctttga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 374 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 374 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Phe Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 375 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 375 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 376 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 376 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 377 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 377 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 378 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 378 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 379 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 379 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 380 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 380 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 381 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 381 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 382 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 382 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 383 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 383 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtggctaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 384 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 384 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Ala Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 385 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 385 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 386 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 386 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 387 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 387 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 388 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 388 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 389 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 389 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactggt gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 390 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 390 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 391 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 391 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttccacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 392 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 392 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe His Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 393 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 393 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgttgaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 394 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 394 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Leu Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 395 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 395 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 396 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 396 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 397 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 397 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctgag gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 398 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 398 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Glu Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 399 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 399 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 400 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 400 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 401 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 401 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 402 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 402 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 403 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 403 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct ggtgaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 404 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 404 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Gly Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 405 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 405 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgagtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 406 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 406 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Ser Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 407 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 407 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc aatggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 408 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 408 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Asn Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 409 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 409 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaagagt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 410 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 410 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Glu Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 411 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 411 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 412 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 412 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 413 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 413 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 414 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 414 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 415 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 415 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 416 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 416 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 417 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 417 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct tatgaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 418 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 418 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Tyr Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 419 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 419 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc ctgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 420 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 420 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Leu Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 421 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 421 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcact gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 422 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 422 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Thr Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 423 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 423 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggttaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 424 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 424 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Val Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 425 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 425 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 426 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 426 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 427 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 427 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 428 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 428 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 429 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 429 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 430 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 430 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 431 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 431 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgcttctgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 432 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 432 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ser Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 433 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 433 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaatggt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 434 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 434 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Trp Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 435 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 435 atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 436 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 436 Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 437 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 437 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 438 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 438 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 439 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 439 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat tcgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcggttgc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 440 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 440 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Val Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 441 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 441 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 442 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 442 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 443 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 443 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 444 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 444 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 445 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 445 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 446 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 446 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 447 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 447 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 448 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 448 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 449 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 449 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct tgggaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 450 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 450 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Trp Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 451 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 451 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 452 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 452 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 453 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 453 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgtgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 454 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 454 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Cys Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 455 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 455 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 456 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 456 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 457 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 457 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtggtc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 458 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 458 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Gly Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 459 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 459 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc tttggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 460 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 460 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Phe Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 461 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 461 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctgtga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 462 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 462 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Cys Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 463 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 463 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttg 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 464 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 464 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Leu 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 465 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 465 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc cagggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 466 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 466 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Gln Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 467 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 467 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 468 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 468 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 469 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 469 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatgcgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 470 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 470 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Met Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 471 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 471 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggagaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 472 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 472 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Glu Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 473 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 473 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct tttgaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 474 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 474 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Phe Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 475 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 475 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 476 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 476 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 477 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 477 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgcttat gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 478 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 478 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Tyr Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 479 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 479 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 480 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 480 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 481 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 481 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tagggctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 482 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 482 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Arg Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 483 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 483 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct cctgaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 484 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 484 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Pro Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 485 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 485 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcgc tatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 486 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 486 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Ala Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 487 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 487 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaaactc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 488 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 488 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Thr His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 489 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 489 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaacatt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 490 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 490 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu His Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 491 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 491 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gaccttgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 492 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 492 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Leu Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 493 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 493 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gactttgaca tggtgcgtaa cgcagtagat 420 gcagctgaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 494 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 494 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Glu Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 495 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 495 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagtggacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 496 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 496 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Trp Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 497 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 497 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct actgaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcaa taaagag 717 <210> 498 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 498 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Thr Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asn Lys Glu 225 230 235 <210> 499 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 499 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaatcgt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 500 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 500 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Ser Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 501 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 501 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagcttgtg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 502 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 502 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Cys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 503 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 503 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 504 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 504 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 505 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 505 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgaataacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 506 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 506 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Asn Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 507 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 507 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgaccgg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccatat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 508 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 508 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Arg Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Ile Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 509 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 509 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 510 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 510 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 511 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 511 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat tccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaatttt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 512 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 512 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Phe Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 513 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 513 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaagggt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 514 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 514 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gly Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 515 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 515 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggataa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 516 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 516 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Asp Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 517 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 517 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 518 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 518 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 519 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 519 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagttt 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 520 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 520 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Phe Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 521 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 521 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agactattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 522 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 522 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Thr Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 523 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 523 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctggggct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 524 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 524 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Gly Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 525 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 525 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccac tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacgcggaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 526 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 526 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Ala Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 527 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 527 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcccggga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 528 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 528 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Arg Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 529 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 529 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc catggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 530 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 530 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg His Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 531 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 531 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gactgtgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 532 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 532 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Cys Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 533 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 533 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gtggaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 534 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 534 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Val Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 535 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 535 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagatgat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 536 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 536 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Asp Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 537 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 537 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcgcgatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 538 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 538 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Ala Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 539 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 539 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgttg ggcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 540 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 540 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Trp Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 541 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 541 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 542 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 542 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 543 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 543 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 544 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 544 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 545 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 545 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaacagt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 546 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 546 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gln Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 547 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 547 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gctgtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 548 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 548 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Cys Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 549 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 549 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tatggctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 550 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 550 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Met Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 551 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 551 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctgtgg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 552 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 552 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Val Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 553 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 553 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctcggg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 554 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 554 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Arg Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 555 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 555 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 556 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 556 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 557 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 557 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tattgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 558 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 558 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Ile Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 559 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 559 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgagtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 560 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 560 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Ser Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 561 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 561 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaacggat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 562 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 562 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Thr Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 563 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 563 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aaccatgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 564 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 564 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn His Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 565 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 565 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaat atctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 566 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 566 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Tyr 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 567 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 567 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaagttt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 568 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 568 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Val Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 569 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 569 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gactctgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 570 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 570 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Ser Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 571 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 571 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 572 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 572 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 573 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 573 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggcgaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 574 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 574 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Ala Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 575 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 575 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 576 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 576 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 577 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 577 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 578 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 578 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 579 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 579 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca cctcggaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 580 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 580 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Ser Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 581 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 581 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagaca gggactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 582 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 582 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp Arg Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 583 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 583 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactagg gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 584 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 584 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Arg Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 585 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 585 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaaa ctctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 586 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 586 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Thr 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 587 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 587 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat tccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaat tgctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 588 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 588 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Leu 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 589 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 589 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctatg gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 590 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 590 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Met Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 591 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 591 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaat ttctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 592 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 592 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Phe 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 593 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 593 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 594 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 594 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 595 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 595 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaagtgat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 596 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 596 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Ser Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 597 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 597 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtga ggcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 598 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 598 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Glu Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 599 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 599 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 attcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 600 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 600 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Ile Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 601 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 601 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tggtgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 602 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 602 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Gly Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 603 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 603 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtactctga aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 604 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 604 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Leu Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 605 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 605 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca atggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaggtt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 606 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 606 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Asn Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gly Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 607 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 607 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 608 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 608 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 609 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 609 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagggc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 610 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 610 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Gly His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 611 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 611 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgc gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 612 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 612 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Ala Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 613 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 613 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaaatgc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 614 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 614 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Met His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 615 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 615 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaac ggctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 616 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 616 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Arg 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 617 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 617 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagcggat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 618 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 618 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Ala Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 619 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 619 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctggtg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 620 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 620 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Gly Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 621 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 621 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaaagtc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 622 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 622 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Ser His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 623 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 623 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 624 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 624 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 625 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 625 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 626 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 626 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 627 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 627 atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300 gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 628 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 628 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 85 90 95 Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 629 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 629 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggggaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 630 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 630 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Gly Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 631 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 631 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcgtgcgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 632 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 632 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Val Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 633 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 633 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacagtgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 634 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 634 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Ser Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 635 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 635 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gtatgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 636 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 636 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Tyr Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 637 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 637 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga aggcgattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 638 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 638 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Ala Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 639 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 639 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaatgtt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 640 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 640 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Cys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 641 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 641 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaatgt acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 642 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 642 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Met Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 643 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 643 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattac gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 644 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 644 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Thr Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 645 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 645 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtca ggcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 646 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 646 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Gln Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 647 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 647 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcacgcgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 648 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 648 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Thr Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 649 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 649 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtaggg 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 650 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 650 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Gly Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 651 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 651 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagcttatg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 652 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 652 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Tyr Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 653 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 653 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacaatgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 654 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 654 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Asn Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 655 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 655 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaggggat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 656 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 656 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Gly Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 657 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 657 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaac atctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 658 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 658 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys His 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 659 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 659 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat ttttgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 660 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 660 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Phe Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 661 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 661 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtctggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 662 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 662 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Leu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 663 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 663 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgg tggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 664 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 664 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Gly Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 665 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 665 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtgg agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcacccac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 666 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 666 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Glu Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 667 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 667 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggatgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 668 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 668 Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 669 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 669 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcaggct 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 670 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 670 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Ala Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 671 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 671 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gactattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 672 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 672 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Thr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 673 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 673 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcgcgg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 674 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 674 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Ala Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 675 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 675 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gccgaatttg gctcgaaggc cctggccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 676 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 676 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ser Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 677 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 677 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac aatgctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 678 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 678 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Asn Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 679 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 679 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctccggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 680 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 680 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Pro Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 681 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 681 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggcttcta ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 682 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 682 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Ser Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 683 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 683 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 684 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 684 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 685 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 685 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cagcgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 686 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 686 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Ala Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 687 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 687 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat caagactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 688 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 688 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Lys 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 689 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 689 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctat gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 690 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 690 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Met Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 691 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 691 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg gtatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 692 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 692 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Gly 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 693 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 693 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagggg 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 694 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 694 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Gly Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 695 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 695 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg ctatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 696 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 696 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Ala 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 697 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 697 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgccatcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 698 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 698 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 His His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 699 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 699 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagcggacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 700 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 700 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Arg Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 701 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 701 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cacagggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 702 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 702 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Gln Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 703 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 703 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcac tatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 704 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 704 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Thr Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 705 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 705 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 agtttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 706 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 706 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Ser Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 707 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 707 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgcaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggcaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gccgaatttg gcgcgaaggc cctggccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggccga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 708 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 708 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 709 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 709 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctaccc atgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 710 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 710 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr His Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 711 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 711 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tacatgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 712 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 712 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Met Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 713 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 713 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag catttggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 714 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 714 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Phe Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 715 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 715 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctaccg ttgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 716 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 716 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Val Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 717 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 717 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atgcgtaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 718 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 718 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Arg Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 719 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 719 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagtcg 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 720 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 720 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Ser Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 721 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 721 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcctgg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 722 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 722 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Leu Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 723 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 723 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat cactactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 724 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 724 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Thr 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 725 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 725 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcgggcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 726 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 726 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Gly His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 727 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 727 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaaa cgctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 728 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 728 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Thr 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 729 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 729 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga tcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 730 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 730 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Asp Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 731 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 731 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat ttcggctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 732 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 732 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Ser Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 733 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 733 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccacactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga tccggag 717 <210> 734 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 734 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile His 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Pro Glu 225 230 235 <210> 735 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 735 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac actgctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 736 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 736 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Thr Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 737 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 737 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaggtttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 738 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 738 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Gly 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 739 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 739 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtgcgact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 740 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 740 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Ala Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 741 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 741 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa ggctattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 742 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 742 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Ala Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 743 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 743 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctcgtg cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 744 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 744 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Arg Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 745 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 745 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttccatgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 746 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 746 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe His Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 747 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 747 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gcttattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 748 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 748 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Leu Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 749 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 749 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcttgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 750 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 750 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Leu Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 751 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 751 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag caagtggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 752 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 752 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Ser Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 753 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 753 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac caggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 754 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 754 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Gln Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 755 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 755 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggca cggttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 756 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 756 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Thr Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 757 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 757 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggttacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 758 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 758 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Val Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 759 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 759 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtagct 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 760 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 760 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Ala Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 761 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 761 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccatactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 762 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 762 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile His 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 763 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 763 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaacagttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 764 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 764 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Gln 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 765 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 765 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct ctgactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 766 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 766 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Leu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 767 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 767 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagcgt 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 768 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 768 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Arg Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 769 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 769 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac agtgctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 770 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 770 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Ser Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 771 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 771 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcctgcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 772 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 772 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Leu His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 773 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 773 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaaa ggctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 774 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 774 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Arg 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 775 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 775 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tattgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 776 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 776 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Ile Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 777 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 777 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgatgggcga taaagag 717 <210> 778 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 778 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Met Gly Asp Lys Glu 225 230 235 <210> 779 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 779 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctggggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 780 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 780 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Gly Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 781 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 781 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaaca agatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 782 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 782 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Lys 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 783 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 783 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttccgggtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 784 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 784 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Arg Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 785 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 785 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgctctcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 786 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 786 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Ser His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 787 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 787 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgactgt atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 788 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 788 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Cys Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 789 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 789 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttctata tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 790 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 790 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Tyr Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 791 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 791 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gggtattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 792 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 792 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Gly Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 793 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 793 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaaca atatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 794 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 794 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asn 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 795 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 795 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaacgtt acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 796 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 796 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Arg Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 797 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 797 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgcta tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 798 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 798 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Ala Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 799 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 799 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctcatgctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 800 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 800 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr His Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 801 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 801 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct tggactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 802 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 802 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Trp Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 803 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 803 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcggcggc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 804 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 804 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Ala Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 805 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 805 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcagtgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 806 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 806 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Ser Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 807 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 807 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctca gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 808 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 808 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Gln Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 809 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 809 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatct gcgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 810 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 810 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Leu Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 811 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 811 atgacgaccc cacacattta tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 812 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 812 Met Thr Thr Pro His Ile Tyr Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 813 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 813 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gaatgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 814 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 814 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Asn Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 815 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 815 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctggc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 816 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 816 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Ala His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 817 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 817 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ctttactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 818 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 818 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Phe 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 819 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 819 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac tatgctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 820 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 820 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Tyr Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 821 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 821 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctgtt gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 822 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 822 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Val Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 823 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 823 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactttt gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 824 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 824 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Phe Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 825 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 825 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga tccggag 717 <210> 826 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 826 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Pro Glu 225 230 235 <210> 827 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 827 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgctggcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 828 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 828 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Trp His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 829 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 829 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctgc tcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 830 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 830 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Ala Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 831 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 831 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgaagaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 832 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 832 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Lys Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 833 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 833 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gtgtgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 834 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 834 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Cys Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 835 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 835 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggctgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 836 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 836 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Ala Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 837 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 837 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 838 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 838 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 839 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 839 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagggtacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 840 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 840 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Gly Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 841 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 841 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttagggatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 842 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 842 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Arg Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 843 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 843 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggatgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 844 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 844 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 845 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 845 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacct gcgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 846 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 846 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Leu Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 847 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 847 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgctt gatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 848 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 848 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Leu Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 849 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 849 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgtt ttatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 850 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 850 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Phe Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 851 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 851 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgctttcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 852 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 852 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Phe His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 853 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 853 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaac atctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 854 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 854 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys His 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 855 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 855 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaagtgttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 856 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 856 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Val 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 857 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 857 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtgtgtaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 858 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 858 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Val Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 859 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 859 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggttattaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 860 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 860 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Tyr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 861 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 861 atgacgaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 862 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 862 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 863 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 863 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cacatggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 864 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 864 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 His Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 865 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 865 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat cagtactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 866 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 866 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Ser 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 867 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 867 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcat gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 868 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 868 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala His Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 869 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 869 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctaccg ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 870 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 870 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Ala Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 871 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 871 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct actactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 872 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 872 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Thr Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 873 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 873 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccttgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 874 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 874 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Leu Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 875 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 875 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga aggcgattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 876 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 876 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Ala Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 877 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 877 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcta tatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 878 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 878 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Tyr Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 879 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 879 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgag ttatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 880 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 880 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Ser Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 881 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 881 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttctgga tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 882 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 882 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Trp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 883 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 883 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ctatgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 884 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 884 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Tyr Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 885 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 885 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtatat 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 886 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 886 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Tyr Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 887 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 887 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcgttg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 888 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 888 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Val Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 889 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 889 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgtc tcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 890 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 890 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Ser His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 891 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 891 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtcaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc tgacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 892 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 892 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Gln Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 893 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 893 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc tcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 894 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 894 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 895 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 895 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg ttcgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 896 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 896 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ser Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 897 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 897 atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 898 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 898 Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 899 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 899 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 cactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 900 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 900 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 His Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 901 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 901 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcacgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 902 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 902 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Thr Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 903 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 903 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acacggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 904 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 904 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Thr Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 905 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 905 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgacctggc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 906 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 906 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Leu Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 907 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 907 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgga tgacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 908 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 908 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Asp Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 909 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 909 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acagggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 910 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 910 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Arg Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 911 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 911 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatggcg 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 912 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 912 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Ala Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 913 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 913 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tcagggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 914 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 914 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Gln Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 915 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 915 atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag caaagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 916 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 916 Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Lys Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 917 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 917 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atggcgcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 918 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 918 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Ala His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 919 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 919 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccact 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 920 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 920 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Thr Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 921 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 921 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cacagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg atatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 922 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 922 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Gln Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 923 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 923 atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 924 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 924 Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 925 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 925 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat caagactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 926 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 926 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Lys 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 927 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 927 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 928 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 928 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 929 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 929 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagtctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 930 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 930 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ser Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 931 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 931 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctacg ttaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 932 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 932 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Val Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 933 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 933 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggca cggttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 934 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 934 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Thr Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 935 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 935 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat cctgactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 936 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 936 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Leu 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 937 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 937 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acagtgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 938 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 938 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Ser Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 939 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 939 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tgctgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 940 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 940 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Leu Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 941 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 941 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgagtatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 942 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 942 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Ser Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 943 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 943 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg accttgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 944 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 944 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Leu Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 945 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 945 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtcaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 946 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 946 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Gln Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 947 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 947 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggca gtgttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 948 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 948 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ser Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 949 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 949 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcattggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 950 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 950 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Ile Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 951 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 951 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca gtaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 952 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 952 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Ser Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 953 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 953 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acgcggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 954 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 954 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Ala Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 955 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 955 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tatgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 956 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 956 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Met Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 957 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 957 atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag caaagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 958 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 958 Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Lys Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 959 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 959 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg actgtgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 960 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 960 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Cys Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 961 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 961 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggttctg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 962 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 962 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly Ser Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 963 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 963 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttggacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 964 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 964 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Leu Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 965 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 965 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat cgttactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 966 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 966 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Val 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 967 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 967 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ctttactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 968 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 968 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Phe 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 969 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 969 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg accatgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 970 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 970 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp His Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 971 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 971 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggttacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 972 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 972 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Val Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 973 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 973 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg accctgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 974 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 974 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 975 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 975 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gagatgacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 976 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 976 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Met Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 977 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 977 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgga tcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 978 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 978 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Asp His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 979 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 979 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgggt 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 980 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 980 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Gly Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 981 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 981 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaact gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 982 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 982 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Leu Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 983 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 983 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactttccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 984 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 984 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Phe Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 985 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 985 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tgcgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 986 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 986 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ala Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 987 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 987 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattat gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 988 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 988 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Met Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 989 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 989 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactatg gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 990 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 990 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr Met Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 991 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 991 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatacggct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 992 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 992 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Thr Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 993 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 993 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatcttgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 994 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 994 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Leu Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 995 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 995 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg gtatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 996 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 996 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Gly 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 997 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 997 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tgtgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 998 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 998 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Val Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 999 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 999 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gacggacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 1000 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 1000 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Thr Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 1001 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 1001 atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60 ggcgacccgc tgcgttcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180 atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240 ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360 tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420 gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600 tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag 717 <210> 1002 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Variant of Purine Nucleoside Phosphorylase from E. Coli <400> 1002 Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 1 5 10 15 Val Leu Met Pro Gly Asp Pro Leu Arg Ser Lys Tyr Ile Ala Glu Thr 20 25 30 Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 35 40 45 Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 50 55 60 Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 65 70 75 80 Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 85 90 95 Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 100 105 110 Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 115 120 125 Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 130 135 140 Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 145 150 155 160 Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 165 170 175 Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 180 185 190 Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 195 200 205 Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 210 215 220 Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu 225 230 235 <210> 1003 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Phosphopentomutase from Escherichia coli <400> 1003 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540 catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780 gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1004 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> Variant of Phosphopentomutase from Escherichia coli <400> 1004 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405 <210> 1005 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Phosphopentomutase from Escherichia coli <400> 1005 atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60 gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120 ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180 ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240 gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300 ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360 gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420 tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480 atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540 catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600 ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660 gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720 accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780 gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840 ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900 aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960 ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020 atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080 cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140 cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200 gaatatggca aagccatgtt c 1221 <210> 1006 <211> 407 <212> PRT <213> Artificial Sequence <220> <223> Variant of Phosphopentomutase from Escherichia coli <400> 1006 Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 1 5 10 15 Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 20 25 30 His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 35 40 45 Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 50 55 60 His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 65 70 75 80 Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 85 90 95 Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 100 105 110 Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 115 120 125 Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 130 135 140 Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 145 150 155 160 Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 165 170 175 Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 180 185 190 Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 195 200 205 Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 210 215 220 Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 225 230 235 240 Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 245 250 255 Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 260 265 270 Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 275 280 285 Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 290 295 300 Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 305 310 315 320 Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 325 330 335 Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 340 345 350 Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 355 360 365 Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 370 375 380 Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 385 390 395 400 Glu Tyr Gly Lys Ala Met Phe 405

Claims

서열 번호 2, 6, 126, 242, 및/또는 684에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리펩타이드 서열, 또는 이의 기능적 단편을 포함하는 조작된 퓨린 뉴클레오사이드 포스포릴라제로서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2, 6, 126, 242, 및/또는 684를 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 2를 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 6에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 6을 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 126에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은

으로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 126을 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 242에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 7, 12, 27, 28, 31, 32, 35, 37, 38, 52, 57, 85, 94, 97, 98, 100, 102, 133, 136, 143, 144, 145, 148, 149, 150, 162, 169, 170, 172, 173, 195/199, 196, 199, 207, 208, 208/238, 209, 210, 211, 212, 213, 214, 215, 217, 219, 220, 221, 223, 224, 227, 235, 및 238로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 242를 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 684에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리펩타이드 서열은 10, 10/133/227, 10/145, 10/145/227, 18, 20, 26, 29, 39/98, 39/133/227, 60, 62, 63, 74, 89, 94, 97, 97/98, 108, 126, 133, 135, 145, 161, 162, 165, 167, 168, 177, 199, 208, 210, 212, 224, 및 227로부터 선택되는 상기 폴리펩타이드 서열 내의 하나 이상의 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열 번호 684를 참조하여 넘버링되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 표 4.1, 5.1, 6.1, 7.1 및/또는 8.1에 제시된 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 서열 번호 4-1002의 짝수 번호 서열에 제시된 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과 동일한 폴리펩타이드 서열을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 서열 번호 2-1002의 짝수 번호 서열 중 적어도 하나에 제시된 폴리펩타이드 서열을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항 내지 제10항 중 어느 한 항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 야생형 이. 콜라이(E. coli) 퓨린 뉴클레오사이드 포스포릴라제에 비해 적어도 하나의 개선된 특성을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제11항에 있어서, 상기 개선된 특성이 기질에 대한 개선된 활성을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제12항에 있어서, 상기 기질이 화합물 3을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제12항에 있어서, 상기 기질이 화합물 4를 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제12항에 있어서, 상기 기질이 화합물 8을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제11항 내지 제15항 중 어느 한 항에 있어서, 상기 개선된 특성이 화합물 1의 개선된 생산을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항 내지 제12항 및 제15항 중 어느 한 항에 있어서, 상기 개선된 특성이 화합물 10의 개선된 생산을 포함하는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항 내지 제17항 중 어느 한 항에 있어서, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제가 정제되는 것인 조작된 퓨린 뉴클레오사이드 포스포릴라제.
제1항 내지 제18항 중 어느 한 항의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 포함하는 조성물.
제1항 내지 제18항 중 어느 한 항의 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 코딩하는 폴리뉴클레오타이드 서열.
적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 코딩하는 폴리뉴클레오타이드 서열로서, 상기 폴리뉴클레오타이드 서열이 서열 번호 1, 5, 125, 241, 및/또는 683에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 포함하고, 상기 조작된 퓨린 뉴클레오사이드 포스포릴라제의 폴리뉴클레오타이드 서열이 하나 이상의 위치에 적어도 하나의 치환을 포함하는 것인 폴리뉴클레오타이드 서열.
서열 번호 1, 5, 125, 241, 및/또는 683에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 포함하는, 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제, 또는 이의 기능적 단편을 코딩하는 폴리뉴클레오타이드 서열.
제20항 내지 제22항 중 어느 한 항에 있어서, 상기 폴리뉴클레오타이드 서열이 제어 서열에 작동 가능하게 연결되는 것인 폴리뉴클레오타이드 서열.
제20항 내지 제23항 중 어느 한 항에 있어서, 상기 폴리뉴클레오타이드 서열이 코돈 최적화되는 것인 폴리뉴클레오타이드 서열.
제20항 내지 제24항 중 어느 한 항에 있어서, 상기 폴리뉴클레오타이드 서열이 서열 번호 3-1001의 홀수 번호 서열에 제시된 폴리뉴클레오타이드 서열을 포함하는 것인 폴리뉴클레오타이드 서열.
제20항 내지 제25항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 발현 벡터.
제26항의 적어도 하나의 발현 벡터를 포함하는 숙주 세포.
제20항 내지 제25항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 숙주 세포.
숙주 세포에서 조작된 퓨린 뉴클레오사이드 포스포릴라제를 생산하는 방법으로서, 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제가 생산되도록 적절한 조건 하에, 제27항 및/또는 제28항의 숙주 세포를 배양하는 단계를 포함하는 생산 방법.
제29항에 있어서, 배양액 및/또는 숙주 세포로부터 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 회수하는 단계를 추가로 포함하는 생산 방법.
제29항 및/또는 제30항에 있어서, 상기 적어도 하나의 조작된 퓨린 뉴클레오사이드 포스포릴라제를 정제하는 단계를 추가로 포함하는 생산 방법.