KR20200098288A - A cosmetic composition for skin regeneration containing a natural extract compound - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin regeneration containing a natural extract composite, which has a skin cell regeneration effect, atopic dermatitis suppression effect, wrinkle ameliorating and whitening effect by containing the natural extract composite consisting of an Abeliophyllum distichum leaf extract, a Codium fragile extract, and a perilla leaf extract. To this end, the present invention provides a cosmetic composition for skin regeneration containing a natural extract composite consisting of 75.0-94.5 wt% of the Abeliophyllum distichum leaf extract 0.5-5.0 wt% of the Codium fragile extract, and 5.0-20.0 wt% of the perilla leaf extract.

Description

A cosmetic composition for skin regeneration containing a natural extract compound}

The present invention is for skin regeneration containing a natural extract complex to have a skin cell regeneration effect, atopic dermatitis suppression effect, wrinkle improvement and whitening effect by containing a natural extract complex consisting of a serrata leaf extract, a hearing extract, and a perilla leaf extract It relates to a cosmetic composition.

Among the major matrix proteins produced by fibroblasts in the skin, collagen is an important protein that accounts for about 30% of the total weight of biological proteins, and forms most of the organic substances contained in skin, bones and teeth, especially in the dermis of the skin. exist. The main functions of collagen include skin mechanical firmness, connective tissue resistance and bonding, support cell adhesion, and induce cell differentiation. Therefore, collagen plays an important role in wound healing, and by promoting the synthesis of collagen in the damaged epithelium, the wound can be quickly recovered without scarring.

Recently, research to regenerate the skin by promoting the production of collagen is being actively conducted, and as part of the research,'a composition for skin regeneration containing pork fat and bamboo salt' is disclosed in Korean Patent No. 10-1337392. .

The above technology relates to a composition containing pork fat and bamboo salt as active ingredients to be effective against skin-related diseases, and to have skin regeneration ability by promoting collagen production.

However, the above technology was very likely to have side effects when applied to the human body as the safety verification for the human body was not properly secured.In particular, there are no operational factors in terms of cosmetic such as skin wrinkle improvement or skin whitening. As a result, not only the related mechanism is not performed, but also the skin regeneration function through collagen synthesis is found to be a problem in that the treatment-related efficacy itself does not work for users suffering from loss of skin tissue at the treatment site or a specific allergic disease.

Korean Patent Registration No. 10-1337392 (2013.11.29. Composition for skin regeneration containing pork fat and bamboo salt) Korean Patent Registration No. 10-1355385 (2014.01.20. Peptide for skin regeneration, cosmetic composition containing the same, and skin regeneration method using the same) Korean Patent Application Publication No. 10-2017-0025367 (2017.03.08. Skin improvement composition)

Accordingly, the present invention is to solve the above problems, and an object of the present invention is to effectively regenerate skin tissues that are lost due to wounds or cell death due to aging, and at the same time relieve itching associated with atopic dermatitis, antioxidants, and anti-oxidants. The aim is to provide a cosmetic composition for skin regeneration containing a natural extract compound that exhibits excellent efficacy in inflammatory action, wrinkle improvement and skin whitening.

The configuration of the problem solving means of the present invention for achieving the above object,

It is characterized in that it consists of 75.0-94.5% by weight of the extract of Misun tree leaves, 0.5-5.0% by weight of the auditory extract and 5.0-20.0% by weight of the perilla leaf extract.

At this time, the Miseon tree leaf extract is pulverized after shading the leaves of Miseon tree to form a powder body, and the powder body is added to 70% ethanol in a weight ratio of 18:1 to 22:1 at 70-80°C. -Prepared by lyophilizing after extracting for 5 hours and filtering the extracted solution with a filter paper, and then concentrating the filtered filtrate at 45-55°C under reduced pressure until it reaches 2-4% of the total volume of the filtrate. desirable.

In addition, the auditory extract is pulverized after the hearing is shaded to form a powder, and the powder is added to 50% ethanol in a weight ratio of 22:1 to 26:1, and extracted for 3 to 5 hours at 80-90°C. The obtained extract solution is filtered through a filter paper, and then the filtered filtrate is concentrated under reduced pressure at 45 to 55° C. until it reaches 2 to 4% of the total volume of the filtrate, and then freeze-dried.

In addition, the perilla leaf extract was pulverized after shading the perilla leaf to form a powder, and the powder was added to 70% ethanol in a weight ratio of 16:1 to 20:1 at 70-80°C for 3-5 It is preferable that the extraction solution obtained by extraction over time is filtered through a filter paper, and then the filtered filtrate is concentrated under reduced pressure until it reaches 2 to 4% of the total volume of the filtrate at 45-55°C, and then freeze-dried. .

On the other hand, the cosmetic composition for skin regeneration containing the natural extract complex according to the present invention consisting of 75.0-94.5% by weight of the serrata leaf extract, 0.5-5.0% by weight of the auditory extract, and 5.0-20.0% by weight of the perilla leaf extract, emollient, antioxidant Agent, suspending agent, foaming agent, surfactant, purified water, ionic or nonionic emulsifier, filler, preservative, vitamin, blocker, wetting agent, dye, pigment, hydrophilic or lipophilic active agent. I can.

In addition, the cosmetic composition for skin regeneration containing the natural extract complex according to the present invention may be made of any one or more formulations selected from lotion, water-soluble liquid, cream, lotion, essence, foundation, shampoo, cosmetic ointment, gel, and pack. .

The cosmetic composition for skin regeneration containing the natural extract complex according to the present invention,

First, it effectively regenerates skin tissues that have been lost due to wounds or died from aging,

Second, it shows excellent efficacy in relieving itching associated with atopic dermatitis, antioxidant, anti-inflammatory action, wrinkle improvement and skin whitening.

Third, there is an effect that the composition composed of the extract of Misun tree leaves, the hearing extract, and the perilla leaf extract can be used in various formulations such as creams, essences, and packs.

1 is a graph showing the effect of inhibiting elastase activity of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
2 is a graph showing the inhibitory effect of collagenase activity of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
Figure 3 is a graph showing the regeneration effect on skin epidermal cells of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
Figure 4 is a graph showing the regeneration effect on the skin dermal cells of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
Figure 5 is a graph showing the secretion inhibitory effect of beta-hexosaminidase (β-hexosaminidase) secretion of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
6 is a graph showing the antioxidant effect of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
7 is a graph showing the ability to inhibit tyrosinase activity for showing the whitening effect of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.
Figure 8 is a graph showing the NO production inhibitory ability to show the inflammation inhibitory effect of a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention.

Hereinafter, a cosmetic composition for skin regeneration containing a natural extract complex according to a preferred embodiment of the present invention will be described in detail.

The cosmetic composition for skin regeneration containing the natural extract complex of the present invention is composed of 75.0-94.5% by weight of misun tree leaf extract, 0.5-5.0% by weight of auditory extract, and 5.0-20.0% by weight of perilla leaf extract.

Abeliophyllum distichum Nakai, which is an object for obtaining the Misun leaf extract, is a plant of the Ashenaceae family and named as Miseon tree because its fruit resembles a round fan, and is the only shrub in the world that lives only in Korea. It is a natural monument of the genus. It contains about 10 times more polyphenols than green tea, and is known to have a large amount of ingredients that have a significant effect on anti-inflammatory, anti-cancer, and antioxidant properties.

According to a preferred embodiment of the present invention, it is preferable that the leaf extract of Misun tree is contained in an amount of 75.0 to 94.5% by weight based on the total weight of the composition. The desired functional action, such as anti-inflammatory, antioxidant action, etc., is sufficiently achieved when the extract of Misun tree leaves exceeds 94.5% by weight, but is uneconomical because it is added more than necessary, and when it is less than 75.0% by weight, the content is insignificant and desired. It is difficult to plan a functional action.

Codium fragile , which is an object for obtaining the auditory extract, is distributed around the world as a mild seaweed, and grows from directly under the low tidal line of the Mangubu to the seabed about 30 cm deep. Recently, research on the efficacy of hearing has been actively conducted, and its usefulness and effectiveness have been proven through several papers. In addition, it has been used as an anthelmintic in folk remedies, promoting bowel activity and improving blood circulation, and treating urinary diseases and dropsy. Hearing contains acrylic acid with antibiotic action, anticoagulant active substances, etc. It has been found to have mutant effect and immune activity. In addition, the hearing uniquely contains hydroxyproline, which is essential for collagen formation, and collagen is an important protein distributed in each connective tissue of the human body, and it is a variable of tissue aging, osteoarthritis, and osteoporosis due to damage to collagen in the tissue. It is known to be the cause of. Therefore, research results showing that hearing improves cartilage regeneration has been published, further supporting the usefulness of hearing.

According to a preferred embodiment of the present invention, the auditory extract is preferably contained in an amount of 0.5 to 5.0% by weight based on the total weight of the composition. If the auditory extract exceeds 5.0% by weight, it is uneconomical because it is added more than necessary, and if it is less than 0.5% by weight, the content of hydroxyrtoline required for collagen formation is insignificant, and skin regeneration is not performed smoothly.

Subject to obtaining the perilla leaf extract Perilla chain (Perilla frutescens var. japonica HARA ) is an annual herbaceous plant cultivated in Asian regions such as Korea, China, and Japan, and contains a large amount of useful components for the human body. Perilla seeds contain a large amount of omega-3 fatty acid, linolenic acid, and are excellent in inhibiting the synthesis of eicosanoids, which cause adult diseases such as high blood pressure and allergic diseases. In addition, perilla leaves contain perillylaldehyde, limonene, and perillaketon as vegetable essential oils.

According to a preferred embodiment of the present invention, the perilla leaf extract is preferably contained in an amount of 5.0 to 20.0% by weight based on the total weight of the composition. When the content of the perilla leaf extract exceeds 20.0% by weight, the composition is not preferable due to the large amount of oil and fat, and when the content is less than 5.0% by weight, the functional action of linolenic acid contained in the perilla leaf does not occur.

According to a further embodiment of the present invention, the Miseon tree leaf extract is pulverized after shading the Miseon tree leaves to form a powder, and the powder is added to 70% ethanol in a weight ratio of 18:1 to 22:1 Then, the extracted solution was extracted at 70-80°C for 3 to 5 hours and filtered through a filter paper, and then the filtered filtrate was concentrated under reduced pressure at 45 to 55°C until it reached 2 to 4% of the total volume of the filtrate. After lyophilization can be prepared.

At this time, extracting for 3 to 5 hours at 70 to 80° C. by adding the powder obtained by crushing the leaves of Misun trees in 70% ethanol in a weight ratio of 18:1 to 22:1 does not destroy the active ingredients from the leaves of Misun trees. It is to maximize the extraction of the active ingredient within the range of the extract. In addition, when the temperature exceeds 80℃ during extraction, a large amount of substances not related to anti-inflammatory and antioxidant activities are extracted, resulting in weakening of the functional function of the leaves There is a concern that the extraction yield is extremely low when it is less than 70 ℃ occurs.

As for the extraction method for extracting the active ingredient from the leaves of the Misen tree, it is preferable to apply a solvent extraction method using 70% ethanol as a solvent, but it is also possible to apply various known extraction methods such as hot water extraction method and supercritical extraction method. Do.

According to a further embodiment of the present invention, the auditory extract is pulverized after shading the hearing to form a powder, and the powder is added to 50% ethanol in a weight ratio of 22:1 to 26:1 to 80- After extracting at 90°C for 3 to 5 hours, the extracted solution was filtered through a filter paper, and then the filtered filtrate was concentrated under reduced pressure until it reached 2 to 4% of the total volume of the filtrate at 45 to 55°C, and then freeze-dried. It can be manufactured.

In another further embodiment, extracting for 3 to 5 hours at 80 to 90° C. by adding the powder obtained by pulverizing the hearing in 50% ethanol at a weight ratio of 22:1 to 26:1 is extracted from the hearing. This is to maximize the extraction of the active ingredient within the range where the active ingredient is not destroyed, and if the temperature for extracting the extract from the hearing exceeds 90°C, there is a risk that the active ingredient contained in the hearing may be lost by high temperature, and 80°C. If it is less than, there may be a problem that the extraction yield is extremely low.

The extraction method for extracting the active ingredient from the hearing is preferably a solvent extraction method using 50% ethanol as a solvent, but it is also possible to apply various known extraction methods such as hot water extraction method and supercritical extraction method.

According to another further embodiment of the present invention, the perilla leaf extract is pulverized after drying the perilla leaf to form a powder, and the powder is added to 70% ethanol in a weight ratio of 16:1 to 20:1 Then, the extracted solution was extracted at 70-80°C for 3 to 5 hours and filtered through a filter paper, and then the filtered filtrate was concentrated under reduced pressure at 45 to 55°C until it reached 2 to 4% of the total volume of the filtrate. After lyophilization can be prepared.

In another further embodiment, the powder obtained by pulverizing perilla leaves in 70% ethanol is added in a weight ratio of 16:1 to 20:1 and extracted for 3 to 5 hours at 70 to 80°C, perilla leaves This is to maximize the extraction of active ingredients such as linolenic acid contained in, and when the temperature for extracting extract from perilla leaves exceeds 80℃, vegetable essential oils such as perilla aldehyde, limonene, and perilla ketone are extracted. It is difficult to achieve the object of the invention, and if it is less than 70°C, the extraction yield is extremely low, and it may be difficult to exhibit the functionality of perilla leaves.

The extraction method for extracting the active ingredient from the perilla leaves is preferably a solvent extraction method using 70% ethanol as a solvent, but it is also possible to apply various known extraction methods such as hot water extraction method and supercritical extraction method. .

On the other hand, the cosmetic composition for skin regeneration containing the natural extract complex according to the present invention consisting of 75.0-94.5% by weight of the extract of Misun tree leaves, 0.5-5.0% by weight of the auditory extract, and 5.0-20.0% by weight of perilla leaf extract, if necessary Emollients, antioxidants, suspending agents, foaming agents, surfactants, purified water, ionic or nonionic emulsifiers, fillers, preservatives, vitamins, blockers, wetting agents, dyes, pigments, hydrophilic or lipophilic actives. It may contain more.

In addition, the cosmetic composition for skin regeneration containing the natural extract complex according to the present invention can be prepared in various formulations depending on the application such as lotion, water-soluble liquid, cream, lotion, essence, foundation, shampoo, cosmetic ointment, gel, pack, etc. Do.

Hereinafter, the present invention will be described in more detail through examples and experimental examples.

[Example 1] Extraction of active substances from leaves of Misun trees

1. Shade-dried and finely crushed Misun tree leaves 2kg put 70% ethanol 40ℓ and extracted for 4 hours at 75 ℃.

2. The extract was filtered through filter paper and then concentrated to 2 L under reduced pressure at 50°C.

3. The concentrate was dried with a lyophilizer to finally obtain 1.02 kilograms of misun leaf extract.

[Example 2] Extraction of effective substances from hearing

1. Put 24 ℓ of 50% ethanol in 1 kg of crushed hearing in the shade and extracted at 85° C. for 4 hours.

2. The extract was filtered through filter paper, and then the extract was concentrated to 1 L under reduced pressure at 50°C.

3. The concentrate was dried with a freeze dryer to finally obtain 27 g of auditory extract.

[Example 3] Extraction of active substances from perilla leaves

1. Shade-dried and finely crushed perilla leaves were added to 2 kg of 70% ethanol 36ℓ and extracted at 75℃ for 4 hours.

2. The extract was filtered through filter paper, and then the extract was concentrated to 2 L under reduced pressure at 50°C.

3. The concentrate was dried with a freeze dryer to finally obtain 430 g of perilla leaf extract.

[Example 4] Preparation of natural extract complex

A cosmetic composition for skin regeneration containing the natural extract complex according to the present invention as an active ingredient by blending the Misun tree leaf extract, hearing extract, and perilla leaf extract obtained in Examples 1 to 3 in the ratio shown in Table 1 below (hereinafter 'Natural extract complex') was prepared and set as a sample to be used in the experiment.

NO Raw material name Mixing ratio (%) One Sunflower Leaf Extract 90 2 Hearing extract One 3 Perilla leaf extract 9

[Experimental Example 1] Wrinkle improvement effect according to natural extract complex treatment

1. Evaluation of the ability to inhibit the Elastase activity of natural extract complexes

In order to evaluate the anti-wrinkle effect of the natural extract complex, the ability to inhibit Elastase activity was confirmed. To 220 µl of a sample diluted in 0.1M Tris-HCl buffer (pH 8.0), 20 µl of 2 mg/ml N-succinyl-Ala-Ala-Ala-p-nitroanilide was added and reacted at 25° C. for 10 minutes. After 20 µl of 1 µg/ml porcine pancreatic elastase (PPE) was added and reacted for 20 minutes at 25° C., the reaction was terminated on ice, and absorbance was measured at 405 nm using a microplate reader. Adenosine was used as a positive control, and the Elastase inhibitory ability was expressed by the decrease in absorbance of the natural extract complex treated and untreated groups.

Referring to Figure 1 to analyze the results of the evaluation of the ability to inhibit the Elastase activity of the natural extract complex, when compared to the treatment of individual materials, the treatment of the natural extract complex exhibited a high inhibitory ability to inhibit the Elastase activity, and the concentration-dependent ability to inhibit the Elastase activity was observed. In addition, when compared with the positive control, Adenosine, the ability to inhibit Elastase activity was highly observed when the natural extract complex was treated, so it can be confirmed that the natural extract complex according to the present invention has a significant effect on wrinkle improvement.

2. Evaluation of the ability to inhibit collagenase activity of natural extract complexes

In order to evaluate the wrinkle improvement effect of the natural extract complex, the ability to inhibit collagenase activity was confirmed. After diluting the sample by concentration, it was measured using a Collagenase Activity Assay kit (Abcam). After adding 2 µl of sample diluted by concentration and 10 µl of Collagenase to 88 µl of the assay buffer, 100 µl of Collagenase Reaction Mix was added to react, and absorbance was measured at 345 nm using a microplate reader. Adenosine was used as a positive control, and the collagenase inhibitory ability was expressed by the decrease in absorbance of the natural extract complex treated and untreated groups.

When analyzing the results of the evaluation of the ability to inhibit Collagenase activity of the natural extract complex with reference to FIG. 2, the treatment of the natural extract complex has a high inhibitory ability to inhibit Collagenase activity when compared to the treatment of individual materials, and a concentration-dependent ability to inhibit Collagenase activity was observed. In addition, compared with Adenosine, which is a positive control, the ability to inhibit Collagenase activity is highly observed when the natural extract complex is treated, so it can be confirmed that the natural extract complex according to the present invention has a significant effect on wrinkle improvement.

[Experimental Example 2] Skin cell regeneration effect according to natural extract complex treatment

1. Evaluation of skin epidermal cell regeneration ability of natural extract complex

The in vitro scratch assay was used to evaluate the skin epidermal cell regeneration ability of the natural extract complex. HaCaT cells (Keratinocytes) were cultured in a DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ and then dispensed into a 6 well plate so that 1×10 ⁶ cells per well, and then cultured for 12 hours. Was completely attached. After scraping the center of the plate using Scratcher (SPL) and treating the sample for 24 hours, the reduction in the scratched area was expressed in %.

When analyzing the results of evaluation of skin epidermal cell regeneration ability of the natural extract complex with reference to FIG. 3, it is observed that the treatment of the natural extract complex has a high effect on the regeneration of the skin epidermal cells when compared with the treatment of individual materials, and concentration-dependent The effect of regeneration of human skin epidermal cells was observed. In addition, it can be seen that the natural extract complex according to the present invention has a significant effect on the regeneration ability of skin epidermal cells, as it is highly observed compared to Adenosine, which is a positive control.

2. Evaluation of skin dermal cell regeneration ability of natural extract complex

The in vitro scratch assay was used to evaluate the skin dermal cell regeneration ability of the natural extract complex. HDF cells (Human Dermal Fibroblast) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then dispensed into 6 well plates to become 5×10 ⁵ cells per well, and cultured for 12 hours. While the cells were completely attached. After scraping the center of the plate using Scratcher (SPL) and treating the sample for 7 hours, the reduction in the scratched area was expressed in %.

When analyzing the results of evaluation of skin dermal cell regeneration ability of the natural extract complex with reference to FIG. 4, it is observed that the treatment of the natural extract complex has a high effect on the regeneration of the skin dermal cells when compared with the treatment of individual materials. A dependent skin dermal cell regeneration effect was observed. In addition, as compared with Adenosine, which is a positive control, it is observed that the natural extract complex according to the present invention has a significant effect on the regeneration ability of skin dermal cells.

[Experimental Example 3] Atopic Dermatitis Inhibitory Effect According to Natural Extract Complex Treatment

The β-hexosaminidase release assay was used to evaluate the degranulation inhibitory effect of the natural extract complex. RBL-2H3 cells (rat basophilic leukemia cells) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then dispensed into 96 well plates so that 2×10 ⁴ cells per well. Cells were completely attached while incubating for hours. Cells in each well were washed twice with Siraganian buffer (biosolution), and then treated with Siraganian buffer and samples in each well, and cultured for 1 hour. Subsequently, the culture was further cultured for 3 hours under stimulation of PMA (50nM) and A23187 (1 μM). After the reaction was terminated on ice, 50 µl of the supernatant was transferred to a new 96 well plate, and 200 µl of 0.1% Triton X-100 was added to Pellet, dissolved, and 50 µl was transferred. Add 50 µl of substate buffer (1mM 4-p-Nitrophenyl-N-acetyl-β-D-glucosaminide, 0.05M sodium citrate, pH 4.5) to a 96 well plate containing the supernatant and pellet, and react at 37°C for 1 hour. Then, 100 µl of Stop solution (0.05M sodium carbonate buffer, pH 10) was added to terminate the reaction, and absorbance was measured at 405 nm using a microplate reader. The secretion rate of β-hexosaminidase was expressed as a percentage after dividing the value of the supernatant by the value of the supernatant and the value of Pellet.

Analyzing the results of the atopic dermatitis inhibitory effect of the natural extract complex with reference to FIG. 5, the treatment of the natural extract complex exhibits a high inhibitory effect on the secretion of β-hexosaminidase as compared to the treatment with individual materials, and a concentration-dependent effect is observed. Became. In addition, as compared to the positive control, Dexamethasone, it was found to be highly observed, and thus it can be seen that the natural extract complex according to the present invention has a significant effect on the inhibitory action of atopic dermatitis.

[Experimental Example 4] Antioxidant Effect of Natural Extract Complex Treatment

In order to evaluate the antioxidant effect of the natural extract complex, the free radical scavenging ability of DPPH was confirmed. 50 µl of a sample diluted in 200 µM DPPH (1,1-diphenyl-2-picryl hydrazyl) 200 µl dissolved in methanol was added and reacted at 37° C. for 30 minutes, and the absorbance was measured at 405 nm using a microplate reader. Ascrobic acid was used as a positive control, and the absorbance reduction rate of the natural extract complex treated and untreated groups was shown.

When analyzing the results of the antioxidant effect of the natural extract complex with reference to FIG. 6, the treatment of the natural extract complex increases the free radical scavenging ability of DPPH and a concentration-dependent effect is observed as compared to the treatment of individual materials. It can be seen that the natural extract complex according to the invention has a significant effect on antioxidant activity.

[Experimental Example 5] Whitening effect according to natural extract complex treatment

In order to evaluate the whitening effect of the natural extract complex, the ability to inhibit Tyrosinase activity was confirmed. 50 µl of a sample and 50 µl of 100 unit/ml Tyrosinase were added to 50 µl of 0.1M phosphate buffer (pH 6.5), and reacted at room temperature for 5 minutes. 100 µl of 1 mM L-DOPA was added and reacted at 37° C. for 30 minutes, and absorbance was measured at 475 nm using a microplate reader. Arbutin was used as a positive control, and the tyrosinase inhibitory activity was expressed by the decrease in absorbance of the natural extract complex treated and untreated groups.

When analyzing the results of the whitening effect of the natural extract complex with reference to FIG. 7, the treatment of the natural extract complex has a high ability to inhibit tyrosinase activity when compared to the treatment of individual materials, and a concentration-dependent ability to inhibit Tyrosinase activity was observed. In addition, as compared to the positive control Arbutin, the ability to inhibit Tyrosinase activity was highly observed when the natural extract complex was treated, so it can be confirmed that the natural extract complex according to the present invention has a significant effect on the whitening action.

[Experimental Example 6] Anti-inflammatory Effect of Natural Extract Complex Treatment

In order to evaluate the anti-inflammatory effect of the natural extract complex, the ability to inhibit NO production was confirmed. After dispensing RAW 264.7 cells (murine macrophages) into a 96 well plate at 2×10 ⁴ cells/well, incubate for 12 hours in a 37°C, 5% CO ₂ incubator to completely attach the cells. Phosphorus LPS was treated in combination at a concentration of 1 μg/ml for 24 hours, and then the supernatant was separated. The content of NO in the separated culture supernatant was measured. For the content of NO, 100 μl of the separated culture supernatant was treated with the same amount of Griess (Sigma) reagent and reacted for 10 minutes, and then absorbance was measured at 517 nm using a microplate reader. The concentration of nitric oxide was calculated by comparing with a standard straight line obtained using sodium nitrite (NaNO ₂ , Sigma).

When analyzing the results of the anti-inflammatory effect of the natural extract complex with reference to FIG. 8, it was observed that when the individual materials and the natural extract complex were treated, NO production was suppressed. In addition, compared with the treatment of individual materials, the treatment of the natural extract complex was observed to increase the NO production inhibitory ability, and it was observed that the concentration-dependent NO production inhibitory ability was observed, so that the natural extract complex according to the present invention has an anti-inflammatory effect. It can be seen that there is a significant effect.

Claims (6)

Misun tree leaf extract 75.0-94.5% by weight, hearing extract 0.5-5.0% by weight, and perilla leaf extract 5.0-20.0% by weight, comprising a natural extract complex comprising a cosmetic composition for skin regeneration. The method of claim 1,
The misun tree leaf extract,
After drying the leaves in the shade to form a powder, the powder was added to 70% ethanol in a weight ratio of 18:1 to 22:1 and extracted for 3 to 5 hours at 70-80°C. After filtration with filter paper, the filtered filtrate is concentrated under reduced pressure until it reaches 2 to 4% of the total volume of the filtrate at 45-55° C., and then freeze-dried to contain a natural extract complex. Cosmetic composition for skin regeneration. The method of claim 1,
The auditory extract,
After the hearing is shaded and pulverized to form a powder, the powder was added to 50% ethanol in a weight ratio of 22:1 to 26:1, and extracted for 3 to 5 hours at 80-90°C. After filtration, the filtered filtrate is concentrated under reduced pressure at 45 to 55°C until it reaches 2 to 4% of the total volume of the filtrate, and then freeze-dried to regenerate skin containing a natural extract complex. Cosmetic composition. The method of claim 1,
The perilla leaf extract,
Perilla leaves are shaded and pulverized to form a powder, and the powder was added to 70% ethanol in a weight ratio of 16:1 to 20:1, and the extract was extracted for 3 to 5 hours at 70-80°C. After filtration, the filtered filtrate is concentrated under reduced pressure until it reaches 2 to 4% of the total volume of the filtrate at 45-55°C, and then freeze-dried to contain skin containing natural extracts. Cosmetic composition for regeneration. The method of claim 1,
The cosmetic composition for skin regeneration,
Emollients, antioxidants, suspending agents, foaming agents, surfactants, purified water, ionic or nonionic emulsifiers, fillers, preservatives, vitamins, blockers, wetting agents, dyes, pigments, hydrophilic or lipophilic actives. A cosmetic composition for skin regeneration containing a natural extract complex, characterized in that it further comprises. The method of claim 1,
The cosmetic composition for skin regeneration,
A cosmetic composition for skin regeneration containing a natural extract complex, characterized in that it has one or more formulations selected from lotion, water-soluble liquid, cream, lotion, essence, foundation, shampoo, cosmetic ointment, gel, and pack.

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