KR20200057306A - Platycodon grandiflorum tea composition with increased the content of platycodin d or platycodin d3, and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a balloon flower tea composition for increasing a content of platycodin D or platycodin D3 and a manufacturing method thereof. The present invention converts platycoside E in triterpenoid saponins, which are active components included in a balloon flower, into platycodin D and platycodin D3 by irradiating microwaves to the balloon flower in a step of processing the balloon flower as a tea composition and activating β-glycosidase. Therefore, the present invention increases a content of saponins effective in treatments of respiratory diseases. The present invention can prevent reduction of the content of the platycodin D and the platycodin D3 depending on time elapsing by including a step of irradiating an electron beam after irradiating the microwaves, sterilizing microorganisms in the balloon flower, and inhibiting activity of the β-glycosidase.

Description

Platycodine D or Platycodin D3 high content of bellflower tea composition and its manufacturing method {PLATYCODON GRANDIFLORUM TEA COMPOSITION WITH INCREASED THE CONTENT OF PLATYCODIN D OR PLATYCODIN D3, AND MANUFACTURING METHOD THEREOF}

The present invention relates to a bellflower tea composition having a high content of platinum or D-platinine D3, and a method for manufacturing the same, more specifically, beta-glycosylation by irradiating microwaves to bellflower in the process of processing bellflower into a tea composition. Platycoside E and Platycodin D3 are replaced by Platycoside E among Triterpenoid saponins, which are active ingredients contained in bellflower by activating a β- Glycosidase. D3) to increase the content of saponins effective for the treatment of respiratory-related diseases, including microbial irradiation and sterilization of microorganisms in bellflower, including the step of irradiating electron beams, and inhibiting the activity of beta-glycosidase, thereby making platinum The present invention relates to a bellflower tea composition having a high content of platinum or D-platinine D3 and a method of manufacturing the same, characterized by preventing the reduction of the content of Dean D and platinum-Din.

The bellflower (platylcodon) is the only perennial herbaceous plant in East Asia belonging to the Campanulaceae family, and is said to be a path when the root of the bellflower is used as a medicine. The scientific name of the bellflower is Platycodon grandiflorum (Jacq.) A. DC, and the herbal name is Platycodon Radix (Sparg SG, Light ME, Staden J. Biological activities and distribution of plant sponins Journal of Ethnopharmacology, (2004), 94, 219 ~ 243 ). The root is used as a medicinal site.

18 types of triterpenoid saponin (platycodin A, C, D, D2, polygalacin D, D2, etc.) were isolated from bellflower roots (Tada A., Kaneiwa Y., Shoji J., Shibata S., Isolation and the structure of platycodin D. Chemical & Pharmaceutical Bulletin, (1975), 23, 2965-2972). It contains polysaccharides and 0.03% of steroids. As polysaccharides, inulin and platycodinin (polysaccharides of about 10 molecules of fructose) are separated and structured. As a Sapogenin, polygalacic acid, platycodigenin, platycogenic acid A, B, C, platycodogenin and prosapogenin are 3-O-β-glucosylplatycodigenin, and steroids are α-spinasterol, Δ7-stigmastenol, α-spinasteryl-β-D-glucoside, The betulin was isolated, and it was also found that anthocyanin and the like existed from the flower of Gil Gyeong (Kim YS, Kim JS, Choi SU, Kim JS, Lee HS, Roh SH, Jeong YC, Kim YK, Ruy SY, Isolation of a new saponin) and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines.Planta Medica, (2005), 71, 566 ~ 568)

Research on the major efficacy of bellflower has been conducted by scientists in Korea and Japan. Until 2000, it has been mainly focused on pharmacological research on anti-inflammatory effects and analysis of bellflower components in bellflower. Afterwards, the effectiveness of the bellflower, such as the protective effect of liver toxicity and the enhancement of immunity, became known, and the potential for cancer prevention and anticancer activity began to emerge strongly in the bellflower. In addition, in vitro and animal experiments, it was recently discovered that the increase in the expression of inflammatory disease genes was significantly suppressed by the bellflower extract and has strong antioxidant efficacy.

The bellflower, which has strong anti-inflammatory and anti-cancer effects, is used for edible and medicinal purposes, and in oriental medicine, white is associated with the lung, so it has a medicinal effect on the lungs and cools the eyes, and the treatment of lung diseases such as cough, cold, and asthma. Has become commercially available. It has the best effect of changing the phlegm and applying it to the symptoms of sea water, dadam, or discomfort to use it as a presentation drug and to treat phlegm and sea caused by external death. . In addition, it is effective in the case of sore throat (excellent effect for treating neck pain), swelling of the migraine and pain, and when the chest and flanks are sore, or when the belly is swelling and sounding. . It is also good for symptoms such as dying, dyspnea, diarrhea, and heavy weight. All of these actions are closely related to the effect of extinguishing the cold energy that remains on the surface of the body by scraping sputum through waste. In addition, the bellflower (桔梗) has a baenong (排膿) action, there is a prescription to treat lung onions (금) in the bellflower (금) Baeksan of Geumgwa summary, and there are also many prescriptions for surgical ong (저) 「健康 寶 鑑」, Bachelor of Education, 2003, 114).

As the recent researches of bellflower have been actively conducted, the pharmacological effects of bellflower saponins include Jinhae, Gundam, and central nervous system inhibitory action, pain relief, antipyretic effect, anti-inflammatory action, anti-ulcer and gastric secretion inhibitory action, anticholinergic action, hypoglycemic action, cholesterol Various activities including metabolic improvement have been reported (Choi JH, Yoo KY, Park OK ,. Lee CH, Won MH, Hwang IK, Ryu SY, Kim YS, Yi JS, Bae YS, Kang IJ, Platycodin D and 2 ″ -O-acetyl -8 polygalacin D2 isolated from Platycodon grandiflorum protect ischemia / reperfusion injury in the gerbil hippocampus.Brain Research, (2009), 1279, 197 ~ 208; Xu BJ, Han LK, Zheng YN, Lee HJ, Sung CK, In vitro inhibitory effect of triterpenoidal saponins from platycodi radix on pancreatic lipase.Archives of Pharmacal Research, (2005), 28, 180-185). In particular, perennial pathogen hydrothermal extracts have been reported to protect against liver damage by carbon tetrachloride and acetaminophen, and have been reported to increase peritoneal macrophage activity and immune activity (Lee, KJ and Jeong, HG Protective effect of Platycodi Radix) on carbon tetrachloride-induced hepatotoxicity.Food Chem. Toxicol., (2002), 40, 517-525).

As a traditional herbal medicine and general food, bellflowers with various activities are increasing in demand for bellflower-related products every year, and technology development is actively underway.

Republic of Korea Patent No. 10-1815547 in the fermentation powder, bellflower powder, youngji extract powder, persimmon extract powder, carrot extract powder, red ginseng concentrate powder and a mixture of fermented red ginseng concentrate powder as an active ingredient causing respiratory disease-causing bacteria Disclosed is a method for manufacturing a bellflower mixed tablet having an antibacterial effect against an antibacterial composition and a food containing the antibacterial composition and respiratory disease-causing bacteria, and a technique related to the bellflower mixed tablet produced by the method. In addition, Republic of Korea Patent Publication No. 10-2011-0075593 contains a mixed extract of bellflower and garlic as an active ingredient, and asthma by further mixing one or more extracts selected from ginseng, radish, goji and sancho, other trace herbal ingredients , Bronchitis, pharyngitis and the like, a technique related to a health functional food composition for the prevention and improvement of respiratory diseases containing bellflower and garlic extract, which can prevent and improve symptoms of respiratory diseases.

However, the bellflower products classified as general foods are not recognized as functional foods such as ginseng, so they cannot increase the added value, and they are not systematically established for the production of products, and the clinical results are correct due to poor homogeneity of the products. It is not getting.

Bellflower is a food that is good for bronchial or lung since ancient times, and is used as a food for treatment of respiratory diseases.

In the case of processing bellflower by the conventional method, most of the processes of heating the bellflower in particular include a lot of heat, and in this process, the destruction of effective saponins becomes so severe that most of the originally contained saponins are destroyed. do. In particular, destruction by heat or fermentation of saponins Platycodin D, Platycodin D3 and Platycosid E, which are effective for respiratory diseases, has been proved by many papers. Therefore, in order to take the basic intake required for the prevention and treatment of respiratory diseases, there is a problem that more bellflowers should be consumed than the general recommended amount.

The intake of Platycodin D, Platycodin D3 and Platycosid E, which are saponins effective for respiratory diseases, is 30 to 60 mg per day. To consume this amount, 5 to 10 g or more of bellflower must be consumed per day. However, considering this amount, saponin is lost during the actual process.

That is, as the effectiveness of the bellflower processing and formulation is reduced by destroying the saponin contained in the bellflower, the effect of the bellflower is enhanced by enhancing the content of saponins effective for respiratory diseases contained in the bellflower. I developed a method to increase the maximum.

Republic of Korea Registered Patent No. 10-1815547 Republic of Korea Patent Publication No. 10-2011-0075593

The present invention has been devised to solve the problems as described above and to provide the necessary technology,

The present invention activates beta-glycosidase ( β- Glycosidase) by irradiating microwaves to bellflower in the process of processing bellflower into a tea composition to form platinum in the active ingredient contained in bellflower, Triterpenoid saponins. The purpose of the present invention is to provide a bellflower tea composition and a method for manufacturing a saponin effective in treating respiratory diseases by converting side E (Platycoside E) to Platycodin D and Platycodin D3. There is this.

In addition, the present invention increases the content of saponins effective for treating respiratory-related diseases by microwave irradiation on bellflower in the process of processing bellflower into a tea composition, and sterilizes microorganisms in bellflower and beta-glycosyl, including a process of irradiating electron beams. A bellflower tea composition having the advantage of preventing the decrease in the content of Platycodin D and Platycodin D3 over time by inhibiting the activity of an azease ( β -Glycosidase) and its manufacturing method There are other purposes to provide.

As one embodiment of the present invention for achieving the above object,

One embodiment of the present invention is a bellflower pre-treatment step of washing and drying the bellflower; A microwave irradiation step of irradiating microwaves to the bellflower pre-processed in the bellflower preprocessing step; An electron beam irradiation step of irradiating the bellflower irradiated with microwaves at least once in the microwave irradiation step; And a pulverization step of crushing the bellflower irradiated with the electron beam in the electron beam irradiation step; provides a method for producing a bellflower tea composition having a high content of platinum-D or platinum-D3.

In the present invention, the bellflower pre-treatment step is characterized in that the dried bellflower is dried at a temperature of 50 to 54 ° C for 70 to 74 hours.

In the present invention, in the microwave irradiation step, it is characterized in that microwaves of 300 MHz to 300 GHz are irradiated to the bellflower for 90 to 150 seconds.

In the present invention, the electron beam irradiation step is characterized in that the step of irradiating the bellflower with an electron beam of 4 to 10 kGy / kg for 50 to 70 minutes is performed twice or more.

In the present invention, the crushing step is characterized in that crushing the bellflower to a size of 2 to 10 mm.

Another embodiment of the present invention provides a bellflower tea composition having a high content of Platycodin D or Platycodin D3, which is produced by the above method.

The bellflower tea composition having a high content of platicodine D or platicodine D3 prepared according to one embodiment of the present invention is irradiated with microwaves to bellflower in the process of processing bellflower into a tea composition to beta-glycosidase ( β- Platycoside E is converted to Platycodin D and Platycodin D3 from Triterpenoid saponins, an active ingredient contained in bellflower by activating Glycosidase). Therefore, it has an advantage of increasing the content of saponins effective in treating respiratory diseases.

In addition, the bellflower tea composition having a high content of platicodine D or platicodine D3 prepared according to an embodiment of the present invention is saponin effective for treating respiratory-related diseases by microwave irradiation to bellflower in the process of processing bellflower into a tea composition. After increasing the content of, sterilizing microorganisms in bellflower, including the process of irradiating an electron beam, and inhibiting the activity of beta-glycosidase ( β- Glycosidase), Platycodin D (Platycodin D) and platinum over time There is an advantage that can prevent the reduction of the content of Dean D3 (Platycodin D3).

1 is a flowchart showing a method of manufacturing a bellflower tea composition having a high content of platicodine D or platicodine D3 according to an embodiment of the present invention for each process step.
FIG. 2 is a photograph showing a state in which a specimen is dissolved in acetonitrile for UPLC measurement and a preparation state for irradiating microwaves to a bellflower using a microwave oven.
Figure 3 is a graph showing the rate of change for the content of Platycodin D, Platycodin D3 and Platycosdide E in the specimen (bellflower tea composition).
4 is a mechanism of a chemical reaction that can occur naturally in the relationship between an organic substance and an acid.
5 is a graph showing the solubility of Platycoside E, Platycodin D and Platycodin D3.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains may easily practice. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part includes a certain component, this means that other components may be further included instead of excluding other components unless otherwise specified.

Throughout the specification of the present invention, when a step is said to be “on” or “before” another step, this is not only the case where one step is in direct time series relationship with the other step, but also with the mixing step after each step. Similarly, the two-step sequence can include the same rights as in an indirect time-series relationship where the time-series sequence can change.

The terms “about”, “substantially”, and the like used throughout the specification of the present invention are used in the sense or close to the value when manufacturing and material tolerances unique to the stated meaning are presented, and the present invention To help understand, accurate or absolute figures are used to prevent unscrupulous use of unintentional infringers by the disclosure. As used throughout this specification, the term “step (to)” or “step of” does not mean “step for”.

The present invention relates to a bellflower tea composition having a high content of platinum or D-platinine D3 and a method for manufacturing the same, and a method for manufacturing a bellflower tea composition having a high content of platinum-D or platinum-coin D3 according to the present invention Silver may include a pre-treatment step (S ₁₀₀ ), a microwave irradiation step (S ₂₀₀ ), an electron beam irradiation step (S ₃₀₀ ) and a crushing step (S ₄₀₀ ).

Hereinafter, a method for producing a bellflower tea composition having a high content of platicodine D or platicodine D3 according to an embodiment of the present invention will be described in detail. The bellflower tea composition (hereinafter, also referred to as a “bellflower tea composition”) in which the content of platicodine D or platicodine D3 according to an embodiment of the present invention is increased may be more clearly understood by a manufacturing method described below.

1 is a flowchart showing a method of manufacturing a bellflower tea composition having a high content of platicodine D or platicodine D3 according to an embodiment of the present invention for each process step.

First, a bellflower preprocessing step may be performed (S ₁₀₀ ).

A bellflower pre-treatment step (S ₁₀₀ ) of washing and drying the bellflower may be performed.

According to an embodiment of the present invention, the bellflower pre-treatment step (S ₁₀₀ ) may be characterized in that the washed bellflower is dried at a temperature of 50 to 54 ° C. for 70 to 74 hours.

In the bellflower pretreatment step (S ₁₀₀ ), it is preferable to dry the washed bellflower at a temperature of 50 to 54 ° C. for 70 to 74 hours, which is when the bellflower is dried at a temperature of less than 50 ° C. for less than 70 hours. This is because the quality of the bellflower tea composition itself may deteriorate because the bellflower is denatured or deteriorated during the subsequent process because it is not sufficiently dried, and when the bellflower is dried for a time exceeding 74 hours at a temperature exceeding 54 ° C. This is because the bellflower may be excessively dried, whereby β- Glycosidase may not be efficiently activated in a subsequent microwave irradiation process.

That is, in the present invention, by specifically limiting process conditions such as temperature and time for drying the washed bellflower in the process of pre-processing the bellflower, it is included in the bellflower by enhancing the activity efficiency of β- Glycosidase in a subsequent microwave irradiation step (S ₂₀₀ ). As it is possible to effectively convert Platycoside E among the active ingredients Triterpenoid saponins to Platycodin D and Platycodin D3, the bellflower tea composition has an advantage of increasing the content of saponins effective for the treatment of respiratory diseases.

Therefore, it is most preferable to dry the washed bellflower at a temperature of 50 to 54 ° C. for 70 to 74 hours in the bellflower pretreatment step (S ₁₀₀ ).

Next, a microwave irradiation step may be performed (S ₂₀₀ ).

The microwave irradiation step (S ₂₀₀ ) of irradiating microwaves to the bellflower pre-processed in the bellflower preprocessing step (S ₁₀₀ ) may be performed.

There are various enzymes in the plant, and under certain conditions such as the presence of temperature, humidity, and microorganisms, fermentation occurs naturally, and all components of the plant undergo hydrolysis, resulting in breakage of interatomic bonds or intermolecular bonds. In addition, pyrolysis in which thermal energy such as direct heat or electromagnetic waves is generated also causes secondary or primary bonds in all molecules of the plant to break. However, even these bonds between molecules or atoms vary depending on the type of bond or the type of bond, and the energy required to break and the type of atmosphere or catalyst. It is possible.

In particular, when the activation of β- Glycosidase is achieved, the temperature in the plant increases by irradiation of microwaves, and since it is possible to remove Glucose one by one, conversion to a desired substance is possible.

The present invention converts Platycoside E of Triterpenoid saponins, an active ingredient contained in bellflower, by activating β- Glycosidase by irradiating bellflower to microwaves, converting Platycoside E into Platycodin D and Platycodin D3, saponins effective for treating respiratory diseases, respiratory diseases contained in bellflower It aims to enhance the content of saponins effective in.

That is, by using a microwave (Microwave) β- Glycosidase enzyme to be smoothly activated by removing one or two of the Glucose present in the sugar portion of Platycoside E may be characterized by converting to Platycodin D and Platycodin D3. As part of a method that promotes the activation of β- Glycosidase enzyme, it is more effective to increase the rotational motion of ether bond using microwave rather than simply increasing the temperature. In other words, heating by general heat causes the entire molecule to be heated and the entire molecule vibrates or rotates, but heating by microwave occurs first in rotational motion in the same part as the ether bond, causing the glucose to fall, which is effective for respiratory diseases such as Platycodin D3 or Platycodin It has the advantage of being effectively converted to Saponins like D.

According to an embodiment of the present invention, in the microwave irradiation step (S ₂₀₀ ), it may be characterized in that the microwave is irradiated with 300 MHz to 300 kHz for 90 to 150 seconds.

In the microwave irradiation step (S ₂₀₀ ), it is preferable to irradiate microwaves of 300 MHz to 300 kHz to the pre-treated bellflower for 90 to 150 seconds, which is when irradiating microwaves of 300 MHz or less to the bellflower for less than 90 seconds. This is because it may cause a problem that cannot effectively activate β- Glycosidase, and when microwaves exceeding 300 에 are irradiated to the bellflower for a time exceeding 150 seconds, the transition from Platycoside E to Platycodin D and Platycodin D3 is complete even after the transition is completed. This is because there is a possibility that other active ingredients may be destroyed due to irradiation with microwaves.

That is, in the present invention, microwaves are irradiated to the pre-treated bellflower, but Platycoside E of Triterpenoid saponins, an active ingredient contained in the bellflower, is enhanced by enhancing the active efficiency of β- Glycosidase to Platycodin D and Platycodin D3, saponins effective for treating respiratory-related diseases. It is characterized by specifically limiting the intensity and irradiation time of the microwave so that it can be effectively converted.

Therefore, in the microwave irradiation step (S ₂₀₀ ), it is most preferable to irradiate microwaves of 300 MHz to 300 kHz to the bellflower pre-treated in the bellflower pre-processing step (S ₁₀₀ ) for 90 to 150 seconds.

In the process of manufacturing a bellflower tea composition according to an embodiment of the present invention, specifically limiting the intensity and irradiation time of microwaves irradiated to the bellflower in the microwave irradiation step (S ₂₀₀ ) [saponin in bellflower according to microwave and electron beam irradiation It is set based on the results of the chemical change observation test (Example 2)], which can be more clearly understood by Example 2 below.

Next, an electron beam irradiation step may be performed (S ₃₀₀ ).

In the microwave irradiation step (S ₂₀₀ ), an electron beam irradiation step (S ₃₀₀ ) of irradiating an electron beam to a bellflower irradiated with microwaves one or more times may be performed.

When the microwave is irradiated to the bellflower, the content of the specific saponin may be increased, but the content of the specific saponin gradually decreases over time due to the action of microorganisms and enzymes remaining inside the bellflower.

Therefore, there is a need to kill microorganisms present inside or outside the bellflower in order to maintain a constant content of a specific saponin with increased content in the bellflower for a long time. However, as a method for killing microorganisms, high heat or chemical treatment is required, but these methods reduce the content of saponin or cannot be used as food.

Accordingly, in the present invention, in the process of processing the bellflower into a tea composition, the microwave is irradiated to the bellflower to increase the content of saponins effective for treating respiratory-related diseases, and sterilizing microorganisms in the bellflower including β- Glycosidase By inhibiting the activity of the Platycodin D and Platycodin D3 content over time was prevented from being reduced.

According to an embodiment of the present invention, in the electron beam irradiation step (S ₃₀₀ ), it may be characterized in that the step of irradiating the bellflower with an electron beam of 4 to 10 kGy / kg for 50 to 70 minutes is performed two or more times.

Although not limited thereto, the electron beam irradiation step (S ₃₀₀ ) may be characterized in that the step of irradiating the bellflower with an electron beam of 4 to 10 kGy / kg for 60 minutes is performed 4 times.

In the electron beam irradiation step (S ₃₀₀ ), it is preferable to irradiate an electron beam of 4 to 10 kGy / kg to a bellflower irradiated with microwaves for 50 to 70 minutes, which irradiates a bellflower with an electron beam of less than 4 kGy / kg for a time of less than 50 minutes. This is because there is a concern that microorganisms in the bellflower cannot be sterilized and the activity of β- Glycosidase cannot be effectively suppressed. In the case of irradiating the bellflower with an electron beam exceeding 10 kGy / kg for a time exceeding 70 minutes, cost and process This is because the production efficiency is reduced due to a problem, and it cannot be used as a food composition.

That is, in the present invention, the activity of β- Glycosidase is inhibited for a considerable period of time by irradiating 4 to 10 kGy / kg of electron beams to the bellflower irradiated with microwaves in the electron beam irradiation step (S ₃₀₀ ) during the bellflower tea composition manufacturing process. It has the advantage of being able to store the finally produced bellflower tea composition for a long time, and also has the advantage of maintaining the content of the effective saponin contained in the bellflower tea composition for a long time.

Therefore, in the electron beam irradiation step (S ₃₀₀ ), it is most preferable to perform the process of irradiating the electron beam of 4 to 10 kGy / kg for 50 to 70 minutes in the microwave irradiated step (S ₂₀₀ ) at least twice. desirable.

In the process of manufacturing a bellflower tea composition according to an embodiment of the present invention, specifically limiting the intensity, irradiation period and number of electron beams irradiated to the bellflower in the electron beam irradiation step (S ₃₀₀ ) [in the bellflower according to the electron beam irradiation] Saponin content observation experiment (Example 3)] was set based on the results, which can be more clearly understood by Example 3 below.

Next, a grinding step may be performed (S ₄₀₀ ).

In the electron beam irradiation step (S ₃₀₀ ), a grinding step (S ₄₀₀ ) of crushing the bellflower irradiated with the electron beam may be performed.

In the present invention, by crushing the bellflower irradiated with microwaves and electron beams to an appropriate size, it can be prepared into a leached bellflower tea composition.

According to an embodiment of the present invention, in the crushing step (S ₄₀₀ ) it may be characterized in that the crushing to be a size of 2 to 10 mm bellflower.

In the crushing step (S ₄₀₀ ), it is preferable to crush the bellflower irradiated with microwaves and electron beams to a size of 2 to 10 mm, which is to prepare a leached bellflower tea when crushing the bellflower to a size of less than 2 mm. Even if the tea composition is packaged in a tea bag container in order to escape from the tea bag container during the leaching process, there is a risk that the tea may not be enjoyed neatly due to the presence of debris in the tea water brewed with the leachable bellflower tea composition. Because it can be difficult. In addition, in the case of crushing the bellflower to a size exceeding 10 mm, the leaching time of the finally prepared leached bellflower tea composition takes a long time, and accordingly, the flavor and flavor of bellflower expressed in the leached bellflower tea composition are reduced. Because this can happen.

Therefore, in the crushing step (S ₄₀₀ ), it is most preferable to prepare a bellflower tea composition by pulverizing the bellflower irradiated with the electron beam in the electron beam irradiation step (S ₃₀₀ ) to a size of 2 to 10 mm.

Although not limited thereto, after the crushing step (S ₄₀₀ ), a packaging step (S ₅₀₀ ) of sealing the bellflower tea composition by crushing the bellflower in the packaging paper may be performed.

That is, the bellflower tea composition prepared in the crushing step (S ₄₀₀ ) may be sealed and packaged as a tea bag, and the bellflower tea composition may be sealed in a tea bag container in a certain amount.

The bellflower tea composition may be packaged in a tea bag container by 1.5 to 2g, the tea bag container may have a pouch shape, and a material capable of communicating with water, such as a synthetic resin material net or paper non-woven fabric so that the bellflower tea composition can be poured into water Can be made of.

In addition, the tea bag container in which the bellflower tea composition is sealed and packaged may be additionally packaged in an outer packaging. The outer wrapper may be provided in a variety of different materials and forms within the scope of protecting the bellflower tea composition in the tea bag container during the distribution process such as paper or synthetic resin sheet.

The leachable bellflower tea composition prepared by the above method can be brewed by immersing it in hot or cold water for a certain period of time, and drinking the concerned tea water. This type of drinking method can enjoy the tea neatly because there is no debris in the brewed tea water, and the convenience of use, storage, and portability can be enhanced to increase the consumer's freshness.

Hereinafter, after preparing a bellflower tea composition according to various forms, an experiment for observing chemical changes of saponins in bellflower according to microwave and electron beam irradiation, an experiment for observing saponin content in bellflower according to electron beam irradiation, and effective saponins contained in teas concerned with bellflower tea composition The content observation experiment was conducted, and the bellflower tea composition according to an embodiment of the present invention can be more clearly understood by the experiment described below.

Preparation of bellflower tea composition

1. Bellflower, reagents and devices

Bellflower

The bellflower specimens were collected at the end of October 2017, washed in a tumbler washer, and then dried and dried at 52 ℃ / 72hr. In addition, for comparison of the content of Saponins, Gangwon-do and Chinese-made products were also purchased among commercially available medicinal bellflowers. To reduce the difference in the amount of saponins eluted due to the difference in size due to physical or growth, each collected bellflower is weighed 2 kg of bellflower each, and then the roots and fines are removed using sex roots, and a scale with an error of 1/100 g is used. , Packed 150g each, using a vacuum packaging machine to make a total of 16 specimens of 8 each.

reagent

Acetic acid: Vinegar, concentration 6 to 7%

Citric acid: Jungbunzlauer (Austria) Manufacture Citric acid anhydride 1Kg

Succinic acid: 99.0% purity

L. Bacillus: Provided by fermentation companies

L. Bacillus natto: Provided by the Microbial Fermentation Promotion Agency

Device

Ultrasonic water heater: Seongdong ultrasonic (4,000ml) production

Incubator: JEIO Tech (IB-15G)

Drying oven: JS Research

Electron beam: Korea Institute of Industrial Technology Yeongcheon, EBILU-10-10, E-beam sterilization system

Microwave oven: LG Electronics MR-305S (750w, 2.45GHz)

2. Composition of bellflower tea prepared by physical method

Physical 1

Manufactured by minimizing the influence of the external environment by immediately vacuum-packing the act of irradiating a certain amount (4.5kGy / kg) of an electron beam using an E-beam device to bellflower up to 4 times every 1 hour Did. Hereinafter, it is referred to as ‘Phy1’.

Physical 2

Each 150g of bellflower was spread evenly on a glass plate, rotated, and then irradiated for 120 seconds or 150 seconds using a microwave oven. To minimize the effect of heat generated in each process, the glass plate was cooled with cold water after each process. It was prepared by returning to room temperature while washing and then executing the following procedure. Hereinafter, it is referred to as ‘Phy2’.

3. Composition of bellflower tea prepared by chemical method

Chemical

Three weak acids that can be used for food were used, but succinic acid with low water solubility was excluded from the test. The three weak acids are shown in Table 1 below. Hereinafter, it is referred to as ‘Chem’.

Acetic acid was purchased from a commercially available vinegar, so it was used as it was, and citric acid was prepared at a concentration of 8% and 10%. 400 g of the prepared acetic acid or citric acid solution was put in an ultrasonic hot water heater and preheated for 20 minutes to maintain a constant concentration and temperature (60 ° C.) of the entire test solution. The bellflower specimens (150g) prepared in advance and vacuum-packed were placed on the prepared trays, and dipping and steaming were performed for a necessary time (30 minutes, 60 minutes, 240 minutes). The specimen was placed in a drying oven to remove odor (residual organic acid) and heated for 45 ° C / 4hr. After completing all the processes, the vacuum packaging was performed again using a vacuum packaging machine to minimize further progress.

4. Composition of bellflower tea prepared by biological method

Biological

Fermentation using microorganisms was made by adding 2 g of L. Bacillus stock solution to 200 g of distilled water to adjust the concentration to prepare a specimen, and proceeded for 24 hours in an incubator adjusted to 42 ° C. The fermented specimen after 24 hours left the slippery substance on the surface, put it in an oven, heated it for 100 ℃ / 3 minutes, killed the microorganism, and then dried it for 45 ℃ / 4hr (42 ℃ for most harmful microorganisms of E. coli. If it is over, it is reported to be killed). For all the finished specimens, vacuum packaging was performed to minimize changes in the Triterpenoid saponins component due to the external environment, and then stored and measured. Hereinafter, it is referred to as 'Bio'.

Test to observe chemical change of saponin in bellflower according to microwave and electron beam irradiation

1. Experimental method

The specimen prepared in Example 1 (bellflower tea composition) was prepared with a solvent of 80% H ₂ O 80% and 20% by weight EtOH (C ₂ H ₅ OH) so that Triterpenoid saponins were most soluble, and the impregnated sample was at room temperature. After allowing to stand for 48 hours, triterpenoid saponins were sufficiently eluted and then measured. The state in which the specimen was dissolved in Acetonitrile for UPLC measurement and the preparation state for irradiating microwaves to bellflower using a microwave oven are shown in the photo of FIG. 2. Measurement of the eluted liquid was performed by UPLC-UV (210 nm), and in order to find out the degree of cleavage between Glucose and Apiose, a secondary measurement was also performed on the Refractive Index (RI).

2. Experimental results

In order to observe the chemical change of saponin in bellflower according to microwave and electron beam irradiation, the results of UPLC measurement for the specimen (bellflower tea composition) prepared in Example 1 are shown in Tables 2 and 3 below.

* Crude: Raw bellflower (1 for Jeju 2 years, 2 for Jeju 3 years),

* AA: Acetic Acid, CA: Citric Acid

* BF: Biological Fermentation

* MO: Microwave Oven

* Platycoside G1 = DeapioPlatycoside E

* PDE: Platycoside E, PD: Platycodin D, PD3: Platycodin D3

As shown in Tables 2 and 3, the measured content of Triterpenoid Saponins was found to be 27% higher than that of the 2-year-old muscle, while the content of Saponins with the Deapio structure was high, whereas Bio, Phy1, Chem In the conversion by, all but the partial increase decreased. In addition, it was confirmed by the Phy2 method that both the total amount of Saponins measured and the amount of Saponins effective in the respiratory system increased. In addition, Platycodin D was found to have little change even when converted using physical and chemical biological methods.

Based on the results of the UPLC experiment, the rates of change for the contents of Platycodin D, Platycodin D3 and Platycosdide E in the specimens (bellflower tea composition) prepared in Example 1 are shown in Tables 4 and 3 below.

In general, 1.8 to 2% of dried bellflower weight is considered to be the content of Crude saponins, and about 30% of the investigated saponins are Platycodin D, Platycodin D3, and Platycosdide E.

As shown in Table 4 above, the reliability of the measured amount of effective saponins is considered to be safe without much difference from the general results of measuring bellflower saponins, but the content of effective saponins in conversion by Ph2 is very high. You can see it appears high.

In other words, the saponins of bellflower are predicted to undergo hydrolysis in which one or two sugars fall from the end of the sugar moiety due to the influence of temperature and time, and the organic acid or residual microorganisms present in the bellflower, and to the microwave (2.45GHz). It was confirmed that the sugar can be converted into Triterpenoid saponins, which are effective for respiratory diseases, and the efficiency can be more than 60%.

3. Analysis of experimental results

Why Crude 1 and Crude 2's Platycosides E detection levels are so low

The prepared specimens were stored for a long time (more than 6 months), so that natural hydrolysis occurred by moisture (H ₂ O) and etherase inside the bellflower or hydrolysis using an organic acid as a catalyst, which was attached to C3 of Platycoside E. Glucose falls out of sugar moiety one by one. When one sugar falls out of the original structure, it is recognized as a different material due to the difference in the reflectance and absorption of UV. If the same standard material is not added in advance, it is not detected by the UV detector.

The types and contents of organic acids contained in bellflower are shown in Table 5 below.

The bellflower contains a small amount of organic acid, and hydrolysis occurs in which the destruction of the Ether bond or the Ester bond is caused by these organic acids. In general chemistry theory, the destruction of Ether bonds is known to be decomposed by strong acids (Hi, HBr, etc.) with halogens, but the hydrolysis of ether bonds occurs very slowly on a small scale even in the presence of weakly acidic organic acids.

The basic decomposition reaction to acetic acid, which is a weak acid (acetic acid dissociates only about 0.1% at a concentration of 0.1 mol), is performed as follows, but only 2.5% of 1 mole of acetic acid actually reacts. Proton thus produced participates in the hydrolysis reaction. Of course, not only acetic acid, Citric Acid or Succinic Acid also plays a role.

[Formula]

과 같은 ether bond에 존재하는 산소의 비공유 전자쌍은 산(Acid)이 수분과 만날 때 발생하는 수소이온(H⁺⁾에 의해 공격을 받아 R`를 떼어내는 대신에 H⁺를 수용하여

로 변하게 된다. 즉 R`이 떨어지는 과정에서 Hydrolysis라 불리는 화학적 변화가 발생한다.

The non-covalent electron pair of oxygen present in the ether bond, such as, is attacked by hydrogen ions (H ⁺⁾ generated when the acid meets moisture, instead of removing R` by accepting H ⁺ ,

Will change to In other words, a chemical change called hydrolysis occurs in the process of R` falling.

에서도 일어난다. 이 반응의 상세한 과정은 화학적으로 충분히 설명될 수 있는 과정이며, 유기물과 산의 관계에서 자연적으로 발생하는 Mechanism의 일환으로 하기 도 4와 같다. 이와 같이, Platycoside E에 Hydrolysis(가수분해)가 일어나면서 Apiose 또는 Glucose의 탈락이 일어나면서 DeapioPlatycoside E(Platycoside G1)로 또는 Platycodin D3로 변하게 되는 것이다.This hydrolysis is an Ester bond

Also happens in The detailed process of this reaction is a process that can be sufficiently described chemically, and is shown in FIG. 4 as part of a mechanism that occurs naturally in the relationship between organics and acids. As described above, when hydrolysis (hydrolysis) occurs in Platycoside E, the dropping of Apiose or Glucose occurs, and it is changed to DeapioPlatycoside E (Platycoside G1) or Platycodin D3.

In general, compared to the detected amount of each Triterpnoid saponins contained in bellflower, it can be concluded that the detection amount was lowered due to the conversion of Platycoside E to Platycoside G1 by Hydrolysis.

Why DeapioPlatycoside E detects high

The increased detection level of Deapioplatycoside E (Platycoside G1) is related to the reason for the very low detection rate of Platycosides E of Crude 1 and Crude 2 above.When the Apiose hanging from the sugar moiety (sugar) of C28 of Platycoside E falls to the end, Deapioplatycoside E Becomes. In other words, Ether Cleavage occurs. The amount of Platycoside G1 (DeapioPlatycoside E) increased as the Glucose of Platycoside E fell due to naturally occurring hydrolysis even for Crude material stored in natural state.

Why Platycoside E detection is particularly low

Platycoside E has the largest molecular weight of 1,548 of the Triterpenoid saponins in bellflowers known to date. In general, the higher the molecular weight, the higher the possibility of degradation. In other words, although the side chain located at the edge of the structure of the molecule is covalently bonded to the main chain, it is moved by translation / rotation / vibration / electron motion, and the electrostatic or physical effects of the molecules other than the molecule itself are affected. Will receive. Of course, it can be considered that the influence of vibration is the greatest in the solid phase. Therefore, it can be inferred that decomposition first occurred in the sugar moiety of Platycoside E, the largest molecule. This is because Platycoside E, as shown in FIG. 5, is a material that is easily soluble in a polar solvent unless it is pure water or EtOH or MeOH.

(a) Biological Conversion

In terms of the amount of each Saponin released, the specimen that was left in the natural state and fermented by the reaction of microorganisms in nature and the enzyme inside the bellflower and the specimen that caused the fermentation reaction by Lacto Bacillus (artificially extracted microorganisms) Although there are differences, it is difficult to give great significance. In addition, the amount of changes in Triterpenoid saponins compared to Crude showed a decrease except for Platycodin D, and the total amount of saponins effective for respiratory diseases also decreased by 27% and 21%. In addition, when fermentation is performed separately from the measurement of the content of triterpenoid saponins, there is a problem in that a sense of rejection due to odor occurs.

(b) Physical Conversion (Electron beam bombarding)

In the method excluding PC3, a decrease in Triterpenoid Saponins was observed overall, and PC3 was found to have a 2% increase in total content compared to Crude.

In the method using PC3, PD3 was increased by 17% and PD by 9%. It is inferred that the amount of electron beam bombardment applied to the process of reaching PC1 to PC4 is greater than that required for the Ether bond cleavage.

(c) Physical conversion (Microwave Oven)

Overall, a significant increase (over 70%) of Triterpenoid saponins occurred. It is presumed that the total energy of the microwaves generated by MO was the closest to the energy that could increase the triterpenoid saponins.

In the MO2 method, it was confirmed that the effective Triterpenoid saponins in the respiratory system increased by 58%. It is also estimated that the total amount of microwave energy applied is close to the appropriate amount for conversion of bellflower triterpenoid saponins.

In the MO2 method, the increase of Platycodin D3 by 173% and the increase of 6% by Platycodin D increased by 27% and 19%, respectively, in MO1. .

(d) Chemical Conversion (with Acetic acid or Citric acid)

It was confirmed that the detection amount of Platycodin D and D3 slightly increased or decreased respectively, and the detection amount of Triterpenoid saponins with a Deapi structure also decreased.

In addition, it was confirmed that the total amount of Saponins measured by the CC5 method increased by 3.2% Platycodin D and Platycodin D3 by 33.8%.

Analysis reason synthesis

Among the triterpenoid saponins contained in bellflower, there are three types of substances that have been found to be effective for respiratory diseases: Platycodin D, Platycodin D3, and Platycoside E. However, the three substances contained in commonly known bellflowers are present in roughly the same proportion, regardless of region, and about 25 to 28% of the content of crude saponins between about 1.8 and 2.0% by weight of dried (less than 7%) bellflower. Shows the share between.

Therefore, as part of a method for increasing the content of Triterpenoid saponins effective for the prevention and treatment of respiratory diseases contained in bellflower, all physical and chemical and biological experiments were conducted to develop a method that can be grafted into the actual industry and has high effectiveness. , When microwave (2.45GHz: 12.2cm wavelength) was investigated for a certain period of time, it was confirmed that conversion was made to the most efficient and high level of Platycodin D and Platycosid D3 and Platycoside E effective for respiratory diseases.

Saponin content observation experiment in bellflower according to electron beam irradiation

Experiment content

Microwaves were added to the dried bellflower to activate β- Glycosidase, and 24 hours later, 10 kGy / kg of an electron beam was irradiated to sterilize the microorganisms inside the bellflower to sterilize β- Glycosidase. After blocking the catalytic role of, an experiment was performed to measure the content of effective saponins.

In the preliminary test, it was confirmed that when an electron beam was applied to a food at an intensity exceeding 10 kGy / kg, it could not be used as a food.

Experimental process

The bellflower raw material is dried at 50 ° C. for 8 hours to maintain a water content of 7% or less, and then left at room temperature for 24 hours. After that, after irradiating the bellflower with an electron beam 4.5kGy / kg twice, the effective saponin content was measured, and the effective saponin content was re-measured in the 1st, 2nd, and 4th weeks to change the effective saponin content in the bellflower. Was observed.

Experiment result

The results of experiments to observe the saponin content in bellflower according to electron beam irradiation are shown in Tables 7 to 10 below.

As shown in Tables 7 to 10, it was confirmed that the contents of Platycoside E, Platycodin D3, and Platycodin D did not change enough to give meaning to changes over time (from 24 hours to 4 weeks (Week)). Accordingly, in the process of processing the bellflower into a tea composition, the microwave is irradiated to the bellflower to increase the content of saponins effective for the treatment of respiratory diseases, and then sterilizing microorganisms in the bellflower, including the step of irradiating electron beams, and beta-glycosidase ( By inhibiting the activity of β -Glycosidase), it was confirmed that the decrease in the content of Platycodin D and Platycodin D3 over time can be prevented.

Experiment for observing the content of effective saponins in tea water in which the bellflower tea composition is brewed

The bellflower tea composition prepared according to one embodiment of the present invention and commercially available three bellflower teas were tested to observe the content of effective saponins contained in tea, and the results are shown in Table 11 below.

As shown in Table 11, after the four types of bellflower tea composition was impregnated into 100 ml of water at 70 ° C., and then left to stand, the content of effective saponin was observed over time, resulting in a bellflower prepared according to one embodiment of the present invention. In the case of the tea composition, it was confirmed that the effective saponin content was not only high, but was effectively brought into the tea water in a short time.

In conclusion, through the results of the experiments of Examples 1 to 4, the bellflower tea composition prepared according to one embodiment of the present invention was irradiated with microwaves to the bellflower in the process of processing bellflower into a tea composition, β − By activating Glycosidase, it converts Platycoside E into Platycodin D and Platycodin D3 among Triterpenoid saponins, which are active ingredients contained in bellflower, and has the advantage of increasing the content of saponins effective for treating respiratory diseases, and irradiating microwaves to treat respiratory diseases. After increasing the effective content of saponin, sterilizing microorganisms in bellflower, including the process of irradiating electron beams, and inhibiting the activity of β- Glycosidase, it has the advantage of preventing the reduction of content of Platycodin D and Platycodin D3 over time. Was confirmed.

As described above, the present invention has been described in detail with reference to examples, but the present invention is not limited to the above embodiments, and may be modified in various ways, and the general knowledge in the art within the technical spirit of the present invention. It is clear that many variations are possible by the possessor. In addition, various forms of substitutions, modifications and changes will be possible by those skilled in the art without departing from the technical spirit of the present invention as set forth in the claims, and this also belongs to the scope of the present invention. something to do.

Claims (6)

A bellflower pre-treatment step of washing and drying the bellflower;
A microwave irradiation step of irradiating microwaves to the bellflower pre-processed in the bellflower preprocessing step;
An electron beam irradiation step of irradiating the bellflower irradiated with microwaves at least once in the microwave irradiation step; And
Method for producing a bellflower tea composition having a high content of Platycodin D or Platycodin D3, characterized in that it comprises; a grinding step of crushing the bellflower irradiated with the electron beam in the electron beam irradiation step. The method according to claim 1,
In the bellflower pre-treatment step,
Method of producing a bellflower tea composition having a high content of Platycodin D or Platycodine D3, characterized in that the washed bellflower is dried at a temperature of 50 to 54 ° C for 70 to 74 hours. The method according to claim 1,
In the microwave irradiation step,
Method of producing a bellflower tea composition having a high content of Platycodin D or Platycodine D3, characterized in that microwaves of 300 MHz to 300 Hz are irradiated to the bellflower for 90 to 150 seconds. The method according to claim 1,
In the electron beam irradiation step,
Method of producing a bellflower tea composition having a high content of Platycodin D or Platycodine D3, characterized in that the process of irradiating a bellflower with an electron beam of 4 to 10 kGy / kg for 50 to 70 minutes is performed twice or more. The method according to claim 1,
In the grinding step,
Method of producing a bellflower tea composition having a high content of Platycodin D or Platycodin D3, characterized in that the bellflower is ground to a size of 2 to 10 mm. Claim 1 to claim 5, characterized in that produced by the method of any one of Platycodin D or Platycodine D3 content of bellflower tea composition.

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