KR101815547B1 - Platycodon grandiflorum mixture tablet with increased antibacterial activity against respiratory disease-inducing bacteria - Google Patents

Abstract

The present invention relates to an antibacterial composition for respiratory disease-causing bacteria containing, as an active ingredient, a mixture of fermented Platycodon grandiflorum powder, Platycodon grandiflorum powder, Ganoderma Lucidum extract powder, persimmon extract powder, carrot extract powder, red ginseng concentrate powder, and fermented red ginseng concentrate powder; food including the antibacterial composition; a method for producing a Platycodon grandiflorum mixture tablet having an antibacterial effect against respiratory disease-causing bacteria; and a Platycodon grandiflorum tablet prepared by the method.

Description

[0002] Platycodon grandiflorum mixture tablets with increased antibacterial activity against respiratory disease-inducing bacteria

The present invention relates to an antimicrobial composition comprising as an active ingredient, a mixture of Rhizobia seed powder, Pedicola powder, Ganoderma extract powder, Sensitized powder, Carrot extract powder, Red ginseng concentrate powder and Fermented red ginseng concentrate powder as an active ingredient and the antimicrobial composition The present invention relates to a method for producing a blend of tablets having antimicrobial effect and a blend of tablets prepared by the above method. The composition of the present invention is useful for the prevention and improvement of respiratory diseases by enhancing the antibacterial activity against respiratory disease- Can be useful as a functional food.

Respiratory diseases include colds, bronchitis, pneumonia, tuberculosis, lung cancer, and chronic bronchitis. A cold is a sudden occurrence of runny nose and nasal obstruction in all age groups. Bronchitis is an infectious disease caused by infection with tuberculosis, when the virus is infected, the virus spreads and becomes acute bronchitis. . Chronic bronchitis is an inflammation of the bronchial bronchus, which causes narrowing of the bronchial mucosa to the fibrous inflammatory reaction, and the secretion of mucous from the mucosal cells is increased, resulting in obstruction of the airway.

A typical and common symptom of respiratory diseases is cough or sputum. Cough is a phenomenon in which the air in the alveoli bursts out through the airways. It is caused by the stimulation of the central nervous system, which is distributed in the airway mucosa of the neck, organs, bronchi, Cough is divided into dryness and dryness. Dryness is not accompanied by sputum. It is caused by stimulation by bronchial hyperemia and edema in bronchial inflammation, and by stimulation of vagal nerve area other than airway. Also called cough. The habit is accompanied by sputum, which is caused by the stimulation of the effusion caused by the inflammation of the bronchi and the lungs and helps to discharge the pathological secretions in the air. Coughing is considered to be an important defense against the intrusion of harmful substances into the lungs. However, when the airway is inflamed, the cough occurs as the disease itself continues to be stimulated. In this way, the original defensive meaning disappears and appears to be a symptom that afflicts the patient. Treatment should remove the cause of the cough, but to prevent frequent cough, take the stability, warm the room, and the nose is to avoid stimulation by breathing. Sputum is a secretion of the airway that is spit out by coughing, and it is also called as sputum or sputum. Originally, it is a protective substance for the airway mucosa, but it can cause pain due to increased secretion due to external environment or disease, or thickening of asthma. Sputum can be divided into two types: water in appearance, mucous, pus-shaped, and blood-mixed. When lung congestion occurs like lung adenocarcinoma, a lot of dilute serous sputum emerges. In the early days of bronchitis, pulmonary tuberculosis, and pneumonia, mucus, bronchiectasis, lung abscess, and lung gangrene are pus-shaped sputum.

A wide range of antibiotics such as penicillin and β-lactam have been used to treat the pathogenic bacteria isolated from patients with respiratory diseases. However, as a result of indiscriminate use of antibiotics, β-lactamase It is known that an antibiotic resistant strain producing a resistant substance is produced.

Platycodon grandiflorum ) is widely grown in mountainous regions of Korea, Japan and China. It is widely used as a medicinal herb in oriental medicine, and also widely used for general food. It contains triterpenoid saponin, carbohydrate and fiber . These saponins have been shown to be useful for the prevention and treatment of various diseases such as diarrhea, gonadal function, central nervous system inhibitory action, acute chronic salt, anti-ulcer and gastric secretion inhibiting action, hypoglycemic action to lower blood pressure, And the number of herbal remedies containing bellflower as a herbal medicine material has various pharmacological activities such as 287 in Donguibogam and 49 in amalgamation. Recently, as a result of verifying various pharmacological effects of bellflower, the cultivation area of bellflower is increasing due to the increase of consumption of bellflower.

Korean Patent No. 1574003 discloses an antimicrobial composition for a respiratory disease-related bacteria containing a fir leaf essential oil, and Korean Patent No. 1291216 discloses an antimicrobial composition containing a grape root extract, a black beech tree and a flower seed extract, However, the present inventors have found that the present invention provides a method for producing respiratory disease-causing bacteria comprising a mixture of a platycodon fermentation powder, bellflower powder, ganoderma extract powder, sensory extract powder, carrot extract powder, red ginseng concentrate powder and fermented red ginseng concentrate powder as an active ingredient And is different from an antimicrobial composition.

Disclosure of the Invention The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method for producing a fermented red ginseng extract, which comprises mixing a mixture of a bellflower powder, a bellflower powder, a ganoderma extract powder, a sensory extract powder, a carrot extract powder, a red ginseng concentrate powder, Analysis of the antimicrobial activity against respiratory disease-causing bacteria revealed that the mixture contained Streptococcus pyrogenes , Klebsiella pneumonia , Staphylococcus aureus , Confirming that the antibacterial activity against Bacterium diphtheria ( Corynebacterium diphtheriae ) is excellent, thereby completing the present invention.

In order to solve the above-mentioned problems, the present invention provides a method for inducing a respiratory disease comprising an effective ingredient of a mixture of a bellflower powder, a bellflower powder, a ganoderma extract powder, a sensory extract powder, a carrot extract powder, a red ginseng concentrate powder and a fermented red ginseng concentrate powder There is provided an antimicrobial composition for bacteria.

In addition, the present invention provides a food comprising the above antimicrobial composition.

Also, the present invention provides a method for producing a granular starch powder, comprising the steps of: (1) adding water to a bellflower powder to sterilize it, adding lactic acid bacteria to the mixture, fermenting and lyophilizing to prepare a bellflower fermented powder; (2) concentrating the ginseng ethanol extract, spray drying and pulverizing to prepare ginseng extract powder; (3) concentrating the persimmon extract, spray-drying and pulverizing to prepare a persimmon extract powder; (4) concentrating the carrot juice extract, spray drying and pulverizing to prepare a carrot extract powder; (5) concentrating the ethanolic extract of red ginseng, spray drying and pulverizing to prepare a red ginseng concentrate powder; (6) adding an enzyme to a diluted red ginseng solution diluted with a red ginseng concentrate, heating the reacted reactant, adding lactic acid bacteria thereto, fermenting, sterilizing, concentrating, spray drying and pulverizing to prepare a fermented red ginseng concentrate powder; (7) A method for preparing a fermented red ginseng extract according to any one of (1) to (5), a ghoulngeum extract powder prepared in the step (2), a sensory powder obtained in the step ) And the fermented red ginseng concentrate powder prepared in the above step (6), and a mixture of the blooming powder, glucose, lactose, vitamin C, lactic acid bacteria powder, lemon concentrated powder, lemon flavor powder, yoghurt powder, hydroxypropylmethylcellulose, Mixing enzyme-treated stevia and menthol to prepare a mixture; And (8) drying the mixed mixture and then tableting the mixture with a tableting machine.

In addition, the present invention provides a bellflower tablet prepared by the above method.

The composition of the present invention is useful for the treatment and prevention of respiratory disease-causing bacteria such as Streptococcus pyrogenes , Klebsiella pneumonia , Staphylococcus aureus or Corynebacterium diphtheriae ), Which is advantageous as a food for prevention or improvement of respiratory diseases.

FIG. 1 is a graph comparing changes in absorbance with time after incubation of Streptococcus pyrogenes culture broth with NB medium supplemented with each sample.
FIG. 2 is a graph comparing changes in absorbance of the NB medium supplemented with Klebsiella pneumonia culture medium after incubation.
FIG. 3 is a graph comparing changes in absorbance of cultured Staphylococcus aureus cultures to NB medium to which each sample is added, according to culture time. FIG.
Fig. 4 is a graph showing the effect of the Corynebacterium < RTI ID = 0.0 > diphtheriae ) culture medium after incubation.

In order to accomplish the object of the present invention, the present invention provides a method for producing a fermented red ginseng extract, which comprises a mixture of Rhododendron berry powder, Platycodi powder, Ganoderma extract powder, Sensitized powder, Carrot extract powder, Red ginseng concentrate powder, There is provided an antimicrobial composition for a disease-causing bacterium.

In the antimicrobial composition of the present invention, the mixture may contain one selected from the group consisting of glucose, lactose, vitamin C, lactic acid bacterial powder, lemon concentrated powder, lemon flavor powder, yogurt powder, hydroxypropyl methylcellulose, The above materials may be further mixed,

More specifically, the mixture comprises 16 to 19% by weight of a bellflower powder, 16 to 19% by weight of a bellflower powder, 1.6 to 2.4% by weight of a ganoderma extract powder, 1.6 to 2.4% % Of red ginseng concentrate, 0.8-1.2 wt% of red ginseng concentrate powder, 0.8-1.2 wt% of fermented red ginseng concentrate powder, 17.3-21.3 wt% of glucose, 18-22 wt% of lactose, 2.5-3.5 wt% of vitamin C, 2.5-3.5 wt% 1.6 to 2.4% by weight of lemon-concentrated powder, 1.6 to 2.4% by weight of lemon fragrant powder, 4 to 6% by weight of yogurt powder, 1.6 to 2.4 parts by weight of hydroxypropyl methylcellulose, 0.1 to 0.3% by weight of enzyme- 0.6 wt%, and more specifically, 17.5 wt.% Of the bellflower powder, 17.5 wt.% Of the bellflower powder, 2 wt.% Of the ginseng extract powder, 2 wt.% Of the ginseng extract powder, 2 wt. 1% by weight of concentrated liquid powder, 1% by weight of fermented red ginseng concentrate powder, 19.3% by weight of glucose, 20% by weight of lactose 3% by weight of vitamin C, 3% by weight of lactic acid bacteria powder, 2% by weight of lemon concentrated powder, 2% by weight of lemon flavor powder, 5% by weight of yogurt powder, 2 parts by weight of hydroxypropylmethylcellulose, 0.2% And 0.5% by weight of menthol.

In the antimicrobial composition of the present invention, the respiratory disease-causing bacteria may be Streptococcus pyrogenes , Klebsiella pneumonia), Staphylococcus aureus (Staphylococcus aureus) or Corynebacterium diphtheria (Corynebacterium diphtheriae ). < / RTI >

In addition, in the antimicrobial composition of the present invention, the platycodon fermentation powder is preferably prepared by adding 8 to 12 times (v / w) water to the platycodon powder, sterilizing the mixture at 110 to 130 ° C for 10 to 20 minutes, (V / w) is added to the bellflower powder and the mixture is sterilized at 121 ° C for 15 minutes. Thereafter, the lactic acid bacteria are added, And fermented at 37 ° C for 72 to 96 hours, and freeze-dried, but the present invention is not limited thereto.

In addition, in the antimicrobial composition of the present invention, the gonad extract powder is preferably extracted with 1 to 3 times at 60 to 80 ° C for 7 to 9 hours by adding 40 to 60% (v / v) The ganoderma ethanol extract may be concentrated to 20 to 30 brix, spray-dried and then pulverized. More preferably, 50% (v / v) ethanol is added to the cut manure, The extract may be concentrated to 25 brix, spray dried and then ground, but is not limited thereto.

In addition, in the antimicrobial composition of the present invention, the sensory powder or carrot extract powder is preferably prepared by adding 8 to 12 times (v / w) water to the cut persimmon or carrot and heating at 70 to 90 ° C for 7 to 9 hours (V / w) is added to the cut persimmon or carrot, and the extract is extracted for 8 hours at 80 ° C. The extracted extract is then concentrated to 20 to 30 brix, spray dried and then pulverized. 25 brix, spray dried and then ground, but is not limited thereto.

In the antimicrobial composition of the present invention, the red ginseng concentrate powder is preferably prepared by adding 50 to 70% (v / v) ethanol to the cut red ginseng and extracting it 1 to 3 times at 70 to 90 ° C for 7 to 9 hours The red ginseng ethanol extract may be concentrated to 20 to 30 brix, spray dried and then pulverized. More preferably, 60% (v / v) ethanol is added to the cut red ginseng and red ginseng ethanol extracted twice at 80 ° C for 8 hours The extract may be concentrated to 25 brix, spray dried and then ground, but is not limited thereto.

Also, in the antimicrobial composition of the present invention, the fermented red ginseng concentrate powder is preferably prepared by adding an enzyme to a diluted red ginseng solution of 8-12 brix diluted with a red ginseng concentrate, reacting the mixture at 45-55 ° C for 66-78 hours And fermented at 34 to 40 ° C for 44 to 52 hours, sterilized at 85 to 95 ° C for 50 to 70 minutes, concentrated to 65 to 75 brix, spray dried and pulverized The enzyme is added to a dilution of 10 brix of red ginseng diluted with red ginseng concentrate, and the reaction product is reacted at 50 ° C for 72 hours. The reaction product is heated, lactic acid bacteria are added thereto, the mixture is fermented at 37 ° C for 48 hours, It may be sterilized at 90 DEG C for 60 minutes, concentrated to 70 brix, spray dried and then pulverized, but is not limited thereto. The enzyme may be Sumizyme, which is a complex enzyme of cellulase, beta-glucanase, and hemicellulase.

The present invention also provides a food comprising the above antimicrobial composition. There is no particular limitation on the kind of the food. Examples of the foods to which the antimicrobial composition can be added include dairy products such as meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen and other noodles, gums, ice cream, Drinks, alcoholic beverages, and vitamin complexes, all of which are processed foods in a conventional sense.

The present invention also relates to

(1) adding water to the bellflower powder to sterilize the mixture, adding lactic acid bacteria to the mixture, fermenting the mixture, and lyophilizing the mixture to prepare a bellflower powder;

(2) concentrating the ginseng ethanol extract, spray drying and pulverizing to prepare ginseng extract powder;

(3) concentrating the persimmon extract, spray-drying and pulverizing to prepare a persimmon extract powder;

(4) concentrating the carrot juice extract, spray drying and pulverizing to prepare a carrot extract powder;

(5) concentrating the ethanolic extract of red ginseng, spray drying and pulverizing to prepare a red ginseng concentrate powder;

(6) adding an enzyme to a diluted red ginseng solution diluted with a red ginseng concentrate, heating the reacted reactant, adding lactic acid bacteria thereto, fermenting, sterilizing, concentrating, spray drying and pulverizing to prepare a fermented red ginseng concentrate powder;

(7) A method for preparing a fermented red ginseng extract according to any one of (1) to (5), a ghoulngeum extract powder prepared in the step (2), a sensory powder obtained in the step ) And the fermented red ginseng concentrate powder prepared in the above step (6), and a mixture of the blooming powder, glucose, lactose, vitamin C, lactic acid bacteria powder, lemon concentrated powder, lemon flavor powder, yoghurt powder, hydroxypropylmethylcellulose, Mixing enzyme-treated stevia and menthol to prepare a mixture; And

(8) drying the mixed mixture, and then tableting the mixture with a tableting machine. The method for producing a blossoming tablet having antibacterial effect against respiratory disease-causing bacteria is provided.

The method for producing a trombiculite mixed tablet of the present invention, more specifically,

(1) Water is added to the bellflower powder at a rate of 8 to 12 times (v / w), sterilized at 110 to 130 ° C for 10 to 20 minutes, added with lactic acid bacteria, fermented at 34 to 40 ° C for 72 to 96 hours, Lyophilization to prepare a bellflower fermented powder;

(2) 40-60% (v / v) ethanol was added to the cut soil and the ethanol extract of the ginseng was extracted 1 ~ 3 times at 60 ~ 80 ℃ for 7 ~ 9 hours, concentrated to 20 ~ 30 brix, spray dried, Preparing a gingival extract powder;

(3) Water was added 8 to 12 times (v / w) to the cut sensation, and the sensory extract extracted at 70 to 90 ° C for 7 to 9 hours was concentrated to 20 to 30 brix, spray dried and pulverized, ;

(4) The carrot juice extracted at 70 to 90 ° C for 7 to 9 hours was added to the cut carrots at 8 to 12 times (v / w), concentrated to 20 to 30 brix, spray dried and pulverized to obtain carrot extract powder ;

(5) 50 ~ 70% (v / v) ethanol was added to the cut red ginseng, and the red ginseng ethanol extracts, which were extracted 1-3 times at 70 ~ 90 ℃ for 7 ~ 9 hours, were concentrated to 20 ~ 30 brix, spray dried, Preparing a red ginseng concentrate powder;

(6) The enzyme was added to 8-12 brix diluted red ginseng diluted with red ginseng concentrate and heated at 45 ~ 55 ℃ for 66 ~ 78 hours. Lactic acid bacteria were added to the reaction mixture and 44 ~ 40 ℃ Fermenting for ~ 52 hours, sterilizing at 85 ~ 95 ° C for 50 ~ 70 minutes, concentrating to 65 ~ 75 brix, spray drying and pulverizing to prepare fermented red ginseng concentrate powder;

(7) 16 to 19% by weight of the bellflowy fermented powder prepared in the step (1), 1.6 to 2.4% by weight of the gingival extract powder prepared in the step (2) 1.6 to 2.4% by weight of the powder, 1.6 to 2.4% by weight of the carrot extract powder prepared in the step (4), 0.8 to 1.2% by weight of the red ginseng concentrate powder prepared in the step (5), and the fermented red ginseng concentrate powder prepared in the step 0.8-3.0 wt.% Of lactic acid bacteria powder, 1.6-3.5 wt.% Of lactic acid bacteria powder, 1.6-2.4 wt.% Of lemon balm powder, 16-19 wt.% Of platelet powder, 17.3-21.3 wt.% Of glucose, 18-22 wt. By weight, 1.6 to 2.4% by weight of lemon fragrant powder, 4 to 6% by weight of yogurt powder, 1.6 to 2.4 parts by weight of hydroxypropylmethyl cellulose, 0.1 to 0.3% by weight of enzyme-treated stevia and 0.4 to 0.6% by weight of menthol, Lt; / RTI > And

(8) drying the mixed mixture and then tableting the mixed mixture by a tableting machine,

More specifically,

(1) Water was added 10 times (v / w) to the bellflower powder and sterilized at 121 ° C for 15 minutes, followed by addition of lactic acid bacteria, fermentation at 37 ° C for 72 to 96 hours, lyophilization, ;

(2) preparing 50% (v / v) ethanol by adding 50% (v / v) ethanol to the cut ground, concentrating the ethanol extract twice at 70 ° C for 8 hours at 25 brix, spray drying and pulverizing to obtain gyoza extract powder;

(3) adding water 10 times (v / w) to the cut sensation, concentrating the extract at 80 ° C for 8 hours to 25 brix, spray drying and pulverizing to prepare a sensory-extract powder;

(4) adding water 10 times (v / w) to the cut carrots, concentrating the extract of carrot root at 80 DEG C for 8 hours at 25 brix, spray drying and pulverizing to prepare carrot extract powder;

(5) preparing a red ginseng concentrate powder by adding 60% (v / v) ethanol to the cut red ginseng, concentrating the ethanol extract twice at 80 ° C for 8 hours at a concentration of 25 brix, spray drying and pulverizing;

(6) The enzyme was added to a 10-brix dilution of red ginseng diluted with red ginseng concentrate, and the reaction product was heated at 50 ° C for 72 hours. The reaction mixture was added with lactic acid bacteria, fermented at 37 ° C for 48 hours, Sterilized for 60 minutes, concentrated to 70 brix, spray dried, and pulverized to prepare a fermented red ginseng concentrate powder;

(7) 17.5% by weight of the bellflowy fermented powder prepared in the above step (1), 2% by weight of the gingival extract powder prepared in the step (2), 2% by weight of the sensuous extract powder prepared in the step (3) 2 wt% of carrot extract powder prepared in step (4), 1 wt% of red ginseng concentrate powder prepared in step (5), 1 wt% of prepared fermented red ginseng concentrate powder and 17.5 wt% A mixture of 3% by weight of glucose, 3% by weight of lactic acid bacteria powder, 2% by weight of lemon concentrated powder, 2% by weight of lemon flavor powder, 5% by weight of yogurt powder and 2 parts by weight of yogurt powder, , 0.2% by weight of enzyme-treated stevia and 0.5% by weight of menthol to prepare a mixture; And

(8) drying the mixed mixture, and then tableting the mixed mixture by a tableting machine.

The present invention also provides a bellflower tablet having an antibacterial effect against respiratory disease-causing bacteria produced by the above method.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example  1. Bellflower blend Tablet  Produce

(1) hot air drying bellflower at 40 ℃ and 10 of water at a Doraji powder pulverized to 70 mesh-fold (v / w) was added and then at 121 ℃ sterilized for 15 minutes, lactic acid bacteria (Lactobacillus plantarum) culture medium (10 ⁶ CFU / mL) was added to the sterilized broth by 3% (v / v), and the mixture was fermented at 37 ° C for 4 days and lyophilized.

(2) 50% (v / v) ethanol was added to the cut manure and the supernatant was centrifuged at 6,000 rpm for 8 hours at 70 ° C. The ethanol extract was centrifuged at 25 brix. Dextrin was added to the concentrated concentrate in an amount of 50% (w / v) relative to the concentrate, spray-dried at 150 to 200 ° C, and pulverized to 40 mesh to prepare ganoderma extract powder.

(3) Water was added 10 times (v / w) to the cut sensation, and the supernatant, which was centrifuged at 6,000 rpm at 80 ° C for 8 hours, was concentrated to 25 brix. 50% (w / v) of dextrin to the concentrate was added to the concentrated concentrate, followed by spray drying at 150 to 200 ° C, followed by pulverization to 40 mesh to prepare a sensate-extracted powder.

(4) Water was added 10 times (v / w) to the cut carrots and centrifuged at 6,000 rpm for 8 hours at 80 ° C. The supernatant was concentrated to 25 brix. To the concentrated concentrate was added 50% (w / v) of dextrin to the concentrate, spray dried at 150-200 ° C, and pulverized to 40 mesh to prepare carrot extract powder.

(5) 60% (v / v) ethanol was added to the cut red ginseng (body: Mitsam = 70:30) and the supernatant obtained by centrifuging 6,000 rpm of the red ginseng ethanol extract twice at 80 ° C for 8 hours was added to 25 brix , Spray-dried at 150 to 200 DEG C, and pulverized to prepare a red ginseng concentrate powder.

(6) Addition of 5% of enzyme (Sumizyme) to 10-brix diluted red ginseng diluted by adding purified water to red ginseng concentrate and reaction at 50 ° C for 72 hours was heated at 90 ° C for 30 minutes, Lactobacillus plantarum (10 ⁶ CFU / mL) was added at 1% (v / v), fermented at 37 ° C for 48 hours, sterilized at 90 ° C for 60 minutes, concentrated to 70 brix, Dried and then pulverized to prepare a fermented red ginseng concentrate powder.

(7) 17.5% by weight of the bellflowy fermented powder prepared in the above step (1), 2% by weight of the gingival extract powder prepared in the step (2), 2% by weight of the sensuous extract powder prepared in the step (3) 2% by weight of the carrot extract powder prepared in the step (4), 1% by weight of the red ginseng concentrate powder prepared in the step (5) and 1% by weight of the fermented red ginseng concentrate powder prepared in the step (6) 17.5 wt% of glucose, 19.3 wt% of glucose, 20 wt% of lactose, 3 wt% of vitamin C, Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus rhamnosus , Streptococcus thermophilus , Bifidobacterium longum, Bifidobacterium lactis mixture of lactic acid bacteria, 10 ⁷ cfu / g or higher), 3 wt%, 2 wt% lemon concentrate powder, 2% by weight of lemon flavor powder, 5% by weight of yogurt powder, hydroxypropyl methyl cellulose, 2 weight parts of enzyme-treated stevia 0.2 weight% and menthol 0.5 weight% were mixed to prepare a mixture.

(8) The mixed mixture was dried at 60 ° C or lower for 5 hours, dried to a moisture content of 11% or less, and compressed into tablets.

Tablet material mixing ratio (% by weight) material Production Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Bellflower fermented powder 17.5 14.5 20.5 35 43 17.5 Bellflower powder 17.5 20.5 14.5 - - 17.5 Ganoderma extract powder 2 One 3 3 - 2 Sensitive extract powder 2 3 One 3 - One Carrot extract powder 2 One 3 - - One Red ginseng concentrate powder One 1.5 0.5 2 - One Fermented red ginseng concentrate powder One 1.5 0.5 - - One Raw extract powder - - - - - One Licorice extract powder - - - - - One glucose 19.3 19.3 19.3 19.3 19.3 19.3 Lactose 20 20 20 20 20 20 Vitamin C 3 3 3 3 3 3 Lactic acid bacteria powder 3 3 3 3 3 3 Lemon concentrated powder 2 2 2 2 2 2 Lemon fragrance powder 2 2 2 2 2 2 Yogurt powder 5 5 5 5 5 5 Hydroxypropylmethylcellulose 2 2 2 2 2 2 Enzyme treatment Stevia 0.2 0.2 0.2 0.2 0.2 0.2 menthol 0.5 0.5 0.5 0.5 0.5 0.5 Sum 100 100 100 100 100 100

1. Antimicrobial effect measurement

We measured an antibacterial effect against bacteria causing respiratory infections, using strain is Streptococcus fatigue to Ness (Streptococcus pyrogenes KCTC 19615), keurep when Ella pneumoniae (Klebsiella pneumonia KCTC 2246), Staphylococcus aureus (Staphylococcus aureus KCTC 1621), Corynebacterium diphtheriae KCTC 3075) was used, and the strain was distributed at the microbiological resource center of the Korea Research Institute of Bioscience and Biotechnology. Each strain was inoculated into NB (nutrient broth) medium, cultured at 35 ° C, and then diluted with sterilized water to a concentration of 10 ² CFU / ml.

To 10 g of sample, add 100 mL of water, extract at 35 ° C for 5 hours, add 10 mL of the filtered supernatant to 90 mL of medium, and add 0.2 mL of each of the prepared 10 ² CFU / mL Followed by incubation at 35 ° C with shaking at 100 rpm. After incubation with time, the absorbance was measured at 660 ㎚ to confirm the degree of mycelial growth. When the absorbance of the culture broth was more than 0.2 at 660 ㎚, it was confirmed that the number of bacterial colonies was more than 10 ⁶ CFU / mL.

Example  1. Mixed platycodon according to the ratio of ingredients Tablet  Antimicrobial activity

Tablets (Comparative Examples 1 to 5), which were prepared by preparing the tablet blend tablet of Preparation Example 1 and the tablet preparation method of Preparation Example 1, and blending the ingredients in the compounding ratios in the preparation of the mixture of the step (7) The antimicrobial activity was measured.

As a result, compared with the control (NB medium, control), the treatment of Preparation Example 1 was about 2.5 times (Fig. 1) for Streptococcus pyrogenes , and for Klebsiella pneumonia about 1.5-fold (Fig. 2), Staphylococcus aureus (Staphylococcus aureus) is about 10 times for the bacteria (Fig. 3), Corynebacterium diphtheria (Corynebacterium diphtheriae bacteria (FIG. 4), and the tablet of Production Example 1 exhibited an excellent antimicrobial effect against the respiratory disease-causing bacteria. In addition, it was confirmed that Preparation Example 1 exhibited the most excellent antibacterial activity as compared with the tablet of the Comparative Examples in which the materials and compounding ratios were different (Figs. 1 to 4).

Example  2. Platycodon mixture according to the ratio of ingredients Tablet  Sensory test

In order to prepare a highly-preferred blossom blotter tablet, the blotch blend tablet prepared by the method of Preparation Example 1 and Comparative Examples was chewed to 20 sensory test agents, and the flavor, taste, Respectively. The scoring criteria are very good; 7 points, good; 6 points, good; 5 points, average; 4 points, a little bad; 3 points, bad; 2 points, very bad; 1 point. The number of samples was changed every 2 hours and the same panel was repeated 3 times. The average score was obtained except for the highest score and the lowest score at each iteration.

Sensory Evaluation of Bellflower Mixed Tablet according to Ingredients Ratio Kinds incense flavor Overall likelihood Production Example 1 5.8 6.4 6.2 Comparative Example 1 5.6 6.0 5.8 Comparative Example 2 5.5 6.1 5.7 Comparative Example 3 5.4 5.8 5.6 Comparative Example 4 5.2 5.4 5.3 Comparative Example 5 5.4 5.3 5.3

As a result, platycodonous mixed tablets prepared with the ingredient ratio of Preparation Example 1 showed the highest preference in flavor, taste and overall acceptability, and it was confirmed that the compounding ratio of Preparation Example 1 was most suitable for tablet preparation using the platycodon fermentation powder there was.

Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. It will be understood that the invention may be modified and varied without departing from the scope of the invention.

Claims (9)

A ginseng extract powder of 1.6 to 2.4 wt%, a ginseng extract powder of 1.6 to 2.4 wt%, a carrot extract powder of 1.6 to 2.4 wt%, a red ginseng concentrate powder of 0.8 to 1.2 wt%, a red ginseng extract powder of 16 to 19 wt% Lactic acid bacteria powder 2.5-3.5 wt.%, Lemon concentrated powder 1.6-2.4 wt.%, Fermented red ginseng concentrate powder 0.8-1.2 wt.% Glucose 17.3-21.3 wt.% Lactose 18-22 wt.% Vitamin C 2.5-3.5 wt. , 1.6 to 2.4% by weight of lemon fragrant powder, 4 to 6% by weight of yogurt powder, 1.6 to 2.4% by weight of hydroxypropylmethyl cellulose, 0.1 to 0.3% by weight of enzyme-treated stevia and 0.4 to 0.6% by weight of menthol A composition for antibacterial activity against respiratory disease-causing bacteria containing an active ingredient. delete delete The method according to claim 1, wherein the respiratory disease-causing bacteria are Streptococcus pyrogenes , Klebsiella pneumonia), Staphylococcus aureus (Staphylococcus aureus) or Corynebacterium diphtheria (Corynebacterium diphtheriae . < / RTI > A food comprising the antimicrobial composition for respiratory disease-causing bacteria according to any one of claims 1 to 4. (1) adding water to the bellflower powder to sterilize the mixture, adding lactic acid bacteria to the mixture, fermenting the mixture, and lyophilizing the mixture to prepare a bellflower powder;
(2) concentrating the ginseng ethanol extract, spray drying and pulverizing to prepare ginseng extract powder;
(3) concentrating the persimmon extract, spray-drying and pulverizing to prepare a persimmon extract powder;
(4) concentrating the carrot juice extract, spray drying and pulverizing to prepare a carrot extract powder;
(5) concentrating the ethanolic extract of red ginseng, spray drying and pulverizing to prepare a red ginseng concentrate powder;
(6) adding an enzyme to a diluted red ginseng solution diluted with a red ginseng concentrate, heating the reacted reactant, adding lactic acid bacteria thereto, fermenting, sterilizing, concentrating, spray drying and pulverizing to prepare a fermented red ginseng concentrate powder;
(7) A method for preparing a fermented red ginseng extract according to any one of (1) to (5), a ghoulngeum extract powder prepared in the step (2), a sensory powder obtained in the step ) And the fermented red ginseng concentrate powder prepared in the above step (6), and a mixture of a blend of a blend of a blotter powder, glucose, lactose, vitamin C, a lactic acid bacterium powder, a lemon concentrated powder, a lemon flavor powder, a yogurt powder, a hydroxypropylmethylcellulose, Mixing enzyme-treated stevia and menthol to prepare a mixture; And
(8) drying the mixed mixture, and then tableting the mixture with a tableting machine, wherein the antibacterial effect of the antibacterial agent on respiratory disease-causing bacteria is produced. [7] The method of claim 6, wherein the mixture of step (7) comprises 16 to 19% by weight of borage fermentation powder, 1.6 to 2.4% by weight of ganoderma extract powder, 1.6 to 2.4% A red ginseng concentrate powder of 0.8-1.2 wt%, a red ginseng powder of 16-19 wt%, a glucose 17.3-21.3 wt%, a lactose 18-22 wt%, a vitamin C 2.5 Of lemon balm powder, 1.6 to 2.4 wt.% Of lemon fragrant powder, 4 to 6 wt.% Of yogurt powder, 1.6 to 2.4 wt. Parts of hydroxypropyl methylcellulose, 2.5 to 3.5 wt. 0.1 to 0.3% by weight of enzyme-treated Stevia and 0.4 to 0.6% by weight of menthol. The method according to claim 6,
(1) Water is added to the bellflower powder and sterilized at 110 to 130 ° C for 10 to 20 minutes, followed by addition of lactic acid bacteria, fermentation at 34 to 40 ° C for 72 to 96 hours, lyophilization to prepare bellflower- step;
(2) preparing ganoderma extract powder by concentrating, spray-drying and pulverizing the ganoderma ethanol extract which has been extracted 1-3 times at 60 to 80 ° C for 7 to 9 hours by adding ethanol to the cut ganoderma;
(3) adding water to the cut sensation, concentrating the sensory extract extracted at 70 to 90 ° C for 7 to 9 hours, spray-drying and pulverizing to prepare a sensate-extracted powder;
(4) adding water to the cut carrots and concentrating the extract of the carrots extracted at 70 to 90 ° C for 7 to 9 hours, spray-drying and pulverizing to prepare carrot extract powder;
(5) preparing a red ginseng concentrate powder by adding ethanol to the cut red ginseng, concentrating the extract of the red ginseng extracted at 1 to 3 times at 70 to 90 ° C for 7 to 9 hours, spray-drying and pulverizing the extract;
(6) The red ginseng diluted red ginseng diluted solution was added with enzyme and heated at 45 ~ 55 ℃ for 66 ~ 78 hours. The lactic acid bacteria were added to the reaction mixture and fermentation was carried out at 34 ~ 40 ℃ for 44 ~ 52 hours Sterilized at 85 to 95 ° C for 50 to 70 minutes, concentrated, spray dried and pulverized to prepare a fermented red ginseng concentrate powder;
(7) 16 to 19% by weight of the bellflowy fermented powder prepared in the step (1), 1.6 to 2.4% by weight of the gingival extract powder prepared in the step (2) 1.6 to 2.4% by weight of the powder, 1.6 to 2.4% by weight of the carrot extract powder prepared in the step (4), 0.8 to 1.2% by weight of the red ginseng concentrate powder prepared in the step (5), and the fermented red ginseng concentrate powder prepared in the step 0.8-3.0 wt.% Of lactic acid bacteria powder, 1.6-3.5 wt.% Of lactic acid bacteria powder, 1.6-2.4 wt.% Of lemon balm powder, 16-19 wt.% Of platelet powder, 17.3-21.3 wt.% Of glucose, 18-22 wt. By weight, 1.6 to 2.4% by weight of lemon fragrant powder, 4 to 6% by weight of yogurt powder, 1.6 to 2.4 parts by weight of hydroxypropylmethyl cellulose, 0.1 to 0.3% by weight of enzyme-treated stevia and 0.4 to 0.6% by weight of menthol, Lt; / RTI > And
(8) drying the mixed mixture, and then tableting the mixture with a tableting machine, wherein the antibacterial effect of the antibacterial agent on respiratory disease-causing bacteria is produced. 9. A bellflower tablet having antimicrobial activity against respiratory disease-causing bacteria produced by the method according to any one of claims 6 to 8.

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