KR101333007B1 - Extracting Method of Dendropanax Morbifera Leveille extract - Google Patents

Abstract

A method of extracting hwangchil wood extract is presented. Hwangchil wood extract extraction method according to the present invention, after washing and cutting the hwangchil wood saplings to prepare a mixture by adding vinegar, sugar components and water to the ground raw material and agitated for 20 to 40 minutes . Thereafter, the mixture used for acupuncture is removed, and only the raw materials are put in water in an pottery jar so that it is fermented at about 18 ° C. for 7 days to 10 days, and the fermented material is placed in an extractor and extracted at low temperature at about 60 ° C.

Description

Extracting Method of Dendropanax Morbifera Leveille extract

The present invention relates to a method of extracting hwangchil wood extract.

Generally, Hwangchil-tree has an international scientific name of Dendropanax, meaning panacea, and is an evergreen broad-leaved tree belonging to the family Ardaceae. Hwangchil trees are planted 8 to 10 years directly from the plant will be 15㎝ from this time, it is possible to harvest hwangchil, and more than 25 years old trees come out of the sap.

Most of the bark is cut in English letter I or V letter, and sap is collected. After 1-2 years, the wound should be healed and collected again. If you collect a large amount at a time without collecting as described above, if you collect about five years, the tree will die.

The sap collection of the imperial tree is limited to certain seasons such as summer from July to September, and the yellow lacquer is obtained in small quantities because it is possible to collect yellow lacquer only during the day from 12:00 to 3:00 pm, due to its scarcity. Only used in

Hwangchil has the function as a medicinal herb that has the effect of coating, aroma function as a scent, lowering heat and eliminating poison, and playing an effect on game, stroke and heat, but the pharmacological effects of each ingredient are as follows.

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α-Cubebene: epilepsy, neuropathy, insomnia, syncope, hysteria, migraine, depression treatment,

β-Elemene: Stimulates the central nervous system and hormone system, used to treat male hormones and depression,

β-Selinene: an effect on appetite stimulants, vomiting and diarrhea, vomiting during pregnancy,

α-Muurolene: used for digestive diseases, tonics, antiperspirants, anti-depressants and sedatives,

Germacrene D: used for neuralgia, dysmenorrhea, headache, hematuria and hemostasis,

β-Sitosterol: the main ingredient in the treatment of prostatic hypertrophy.

Such hwangchil has been attempted to be used as a functional drink, medicine or hangover drink.

The manufacturing method of the health beverage using the Hwangchil undiluted solution according to the prior art is to Korea Patent Application No. 10-2002-0009932, using a Hwangchil undiluted solution obtained by wounding the bark of the Hwangchil wood, 10 to 20 parts by weight of ethanol The mixture obtained by adding 0.2 to 1 parts by weight of the juvenile undiluted solution was added to 1000 parts by weight of water together with 5 to 15 parts by weight of the leaves, stems, and roots of the dried Hwangchil tree, and then heated at 70 to 100 ° C. for 1 to 3 hours. It is cooled to room temperature and filtered.

At this time, 1-5 weight part of licorice and 1-5 weight part of round roots can be added to water, and it can heat.

However, the manufacturing method of the health beverage using the hwangchil undiluted solution according to the prior art has a disadvantage in that it is difficult to be commercialized in the manufacturing economy due to the disadvantage of its scarcity of pickling.

In addition, the manufacturing method of the health beverage according to the prior art is inconvenient and difficult to manufacture, as the ethanol addition and dissolution process to further remove the strong aroma and bitter taste of the Hwangchil undiluted solution.

The manufacturing method of liquor using Hwangchil wood according to the other prior art is to the Republic of Korea Patent Application No. 10-2002-0072611, the first process of washing and completely drying the stem and / or bark of the Hwangchil wood and pulverized ; A second process of blending the undifferentiated hwangchil wood material and alcohol at a ratio of about 1: 2, and aging for about two to three months while maintaining a temperature of 12 to 15 degrees; The leaching stock solution of the second step and distilled water are mixed at a ratio of about 3: 2, and the third step of fermenting at room temperature for about two to three months is to allow the liquor to contain the active ingredient of hwangchil wood. In the manufacturing method of the conventional liquor, at least one of honey, yellow sugar, licorice, and wolfberry is contained within about 10% of the leaching solution.

However, the manufacturing method of the liquor using the hwangchil wood according to the other prior art is used to produce liquor using the stem or bark of the hwangchil wood, but it takes several months (for example, 2-3 months) when forming the leaching solution in the manufacturing process In addition, it takes several months for fermentation after the formation of the leaching liquor, which makes it very difficult to apply to drinking and various dishes, and it does not suggest any method for removing strong aroma and bitter taste that cannot be applied to food. There is a disadvantage that can not be applied to food additives.

The manufacturing method of the health drink or liquor mentioned in the prior art are all for using only the components thereof, there is a difficulty in the collection method or takes a long time during the refining technique.

In addition, in the previous patent application No. 10-2009-0118164 of the inventors of the present application, after washing the whole hwangchil wood, vinegar, sugar and water mixed and precipitated, and precipitated, the ratio of the precipitated mixture and water to 1: 3 Dilution to prepare a dilution, and the dilution is heated for 24 hours at a temperature of 100 ~ 120 ℃ and then cooled and filtered at room temperature to extract the extract using Hwangchil wood to produce a dedicated extract for cooking yellow hwangchil and cooking method using the same There is a bar.

However, since this method uses a method of extracting the yellow lacquer extract by heating after cooling at high temperature, there is a problem in mass extraction due to high energy consumption by maintaining nutrients at a high temperature and maintaining a temperature of 100 ° C. or higher for 24 hours. There was a need for an extraction method in which the nutrients of the yellow lacquer tree were not destroyed and energy was saved.

An object of the present invention devised to solve such a problem is to provide a method for extracting hwangchil wood extract which can have a unique pharmacological function of hwangchil wood without destroying nutrients.

According to the spirit of the present invention for achieving the object as described above, the method of extracting the hwangchil wood according to the present invention, by washing the hwangchil wood with water, washing step (S11) to remove the soil, dust, foreign matters, Step wood pulverization step (S12) to make the hwangchil wood wood into a pulverized raw material of 0.05cm ~ 3cm size, the pulverized raw material is added to the mixed liquid by adding vinegar, sugar and water for 20 minutes to 40 minutes Step S14, removing the mixed liquid used for the acupuncture and leaving only the ground raw material (S15), removing the mixed liquid and leaving the remaining raw material after the grinding soaked in water in a pot Put into fermentation at 15 ℃ ~ 25 ℃ for 7 days to 10 days (S16), put the fermented broth fermented together with the raw material and water after the grinding in an extractor to extract at low temperature 55 ℃ ~ 65 ℃ System (S17), and a step (S18) was concentrated to a low temperature between 60 ℃ ~ 70 ℃ the extracted Extract. However, Onggi used for Hwangchil fermentation is limited to jars made of highly breathable ocher and clay.

Preferably, the acupuncture step (S14) is the addition of the vinegar, sugar components and water to agitate the pulverized raw material for 28 minutes to 32 minutes to the mixed liquid, the fermentation step (S16) is a pulverized after the grinding Fermented raw materials to the pottery jar soaked in water for 7 days to 10 days at 17 ℃ ~ 19 ℃, the low temperature extraction step (S17) extracts the fermentation broth fermented together with the raw material and water after the grinding Put in and extract at 58 ℃ ~ 62 ℃.

In one embodiment, the method of extracting the hwangchil wood extract, after the low temperature concentration step, further comprises the step of product packaging the concentrated extract.

As described in detail above, the method of extracting the hwangchil wood of the present invention enables the general public to easily ingest and use the hwangchil component, and the nutrients of the hwangchil tree can be extracted as the extract as it is, so as to extract the extract through fermentation, and low temperature extraction. By extracting the extract through the process to extract the extract at a high temperature compared to the process of extracting the extract without extracting the nutrients, there is an aroma or pharmacological effect as a relaxing scent.

Thus extracted hwangchil wood extract may be used as a health food or food cooking.

1 is a block diagram showing a method of extracting hwangchil wood according to a preferred embodiment of the present invention.

The details of other embodiments are included in the detailed description and drawings.

Advantages, features, and methods of accomplishing the present invention will be apparent with reference to the embodiments described below in conjunction with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art. To fully disclose the scope of the invention to a person skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout.

Hereinafter, with reference to the accompanying Figure 1 will be described in detail with respect to the extract of Hwangchil wood according to a preferred embodiment of the present invention.

In the drawings, Figure 1 is a block diagram showing a method of extracting the hwangchil wood according to a preferred embodiment of the present invention.

Referring to Figure 1, the method of extracting the hwangchil wood extract of the present invention, the first step of preparing the hwangchil wood, the step of washing the hwangchil wood (S11), crushed hwangchik wood (S12), vinegar, sugar components, water mixing step (S13 ), Acupuncture step (S14), after the acupuncture mixed liquid removal step (S15), fermentation step (S16), low temperature extraction step (S17), concentration step (S18) and the product packaging step (S19).

In the step Hwangchil wood cleaning step (S11) to wash the cut hwangchik wood with water, to remove soil, dust, foreign matter.

Hwangchil wood crushing step (S12) is to crush the hwangchil wood to 0.05 ~ 3cm size to make a crushed Hwangchil wood raw material. This is a method for maximizing the extraction performance while striking the hwangchil wood itself in the following mixed liquid.

In the vinegar, sugar component, and water mixing step (S13), a mixed liquid is prepared by mixing at a ratio of 400 ml of pulverized raw material 10 kg vinegar, 2 kg of sugar component, and 30 L of water.

In the acupuncture step (S14), the liquid mixed with vinegar, a sugar component and water to the above raw materials is aerated for about 30 minutes. Preferably it may be acupuncture for 20 to 40 minutes, and may be acupuncture for 28 to 32 minutes. The reason why the raw materials are immersed in the mixed liquid containing vinegar, sugar components and the like is for non-heat treatment sterilization of the yellow lacquer material. In other conventional techniques, it is common to treat at high temperature during sterilization, but in this case, there is a problem of destroying not only the sterilization but also the yellow lacquer material itself. .

In addition, the other reason of the present invention of the Acupuncture is to remove the contaminants remaining in the raw material of the Hwangchil wood at the same time to include the vinegar, sugar components in the raw material, Hwangchil wood is affected by the vinegar and sugar components absorbed during acupuncture When fermentation removes bitter taste, it is easy to break down the unique ingredient (nutrition) of Hwangchil. The efficacy of the extract according to the addition of vinegar, sugar, etc. will be described in more detail below.

The necessity of acupuncture stage is the result of dozens of experiments over a long period of time, and without going through the acupuncture stage, the fermentation process does not remove the bitterness of Hwangchil-tree.

In the agitated mixture removal step (S15), the mixed liquid used for the acupuncture is removed and only the raw material is left.

In the fermentation step (S16) put the raw materials remaining after the acupuncture in the Onggi jar put the water so that the raw material is submerged in water and fermented at about 18 ℃ about 7 ~ 10 days. At this time, the temperature to be fermented can be set to 15 ° C ~ 30 ° C, preferably between 17 ° C and 19 ° C.

In the present invention, the fermentation is put in an Onggi jar so that the fermentation effect is faster and the fermentation speed is faster than the fermentation in a container without pores, thereby shortening the duration of fermentation. In addition, since only a small amount of vinegar, sugar components and water absorbed in the hwangchil during fermentation, the material can be minimized, and the fermentation period is 7 days to 10 days, so that mass production is possible.

The reason that Onggi is effective for fermentation of Hwangchil is that pores exist in Onggi's tissue. These pores may be permeable to air, but not so small as water to permeate, and adequate oxygen from outside may be continuously supplied into the pot. In contrast, other containers, such as glass bottles, do not have any air permeability, which prevents the acetobacter bacterium from vinegar from being the minimum required condition. Due to the presence of these pores, the inside of Onggi is equipped with suitable conditions for fermentation. Onggi has the effect of shortening fermentation period. However, separate management is needed to maintain these effects. As described in 'Study on Marketing Strategies for Cultural Commercialization of Onggi, 18p', the actual experiment was performed as the air inside the pot was circulated, exposing wastes in the bowl to the outside of the bowl, acting as a filter and conserving water for a long time. Even in the process, small amounts of invisible impurities are discharged to the outside through the pores of Onggi, which prevents the supply of oxygen by blocking the pores of Onggi. To solve this problem, the outside of pottery must be cleaned at least 2-3 times a day during fermentation to keep oxygen supply smooth.

  In addition, Onggi presents the best conditions for fermentation of Hwangchil with its moisture retention function, neutralization of odor, and heat storage effect to maintain proper temperature for Hwangchil fermentation.

In the low temperature extraction step (S17), the fermented material is placed in an extractor and extracted at low temperature at about 60 ° C. The low temperature extraction temperature is preferably extracted at 55 ° C to 65 ° C or 58 ° C to 62 ° C.

In the concentration step (S18), the extracted yellow lacquer tree extract is concentrated, and in the product packaging step (S19), the concentrated extract is packaged for sale, for example, to complete the extraction process.

When using the extract method of the hwangchil wood extract according to the present invention, the nutrients of the hwangchil wood can be extracted as the extract as it is to extract the extract through fermentation, compared to the process of extracting the extract at high temperature through a low temperature extraction process There is almost no nutrient destruction.

Thus extracted hwangchil wood extract may be used as a health food, fragrance raw material, beverages, etc., or may be used for food cooking.

The extracted Hwangchil wood extract is used for cooking

An example of the method is as follows.

After preparing the hwangchil wood extract, when the extracted extract is added to the cooking, it can be combined with other food ingredients to exhibit the unique color, aroma and taste of the food. Hwangchil wood extract according to the present invention uses the raw material prepared by crushing the wood as described above, using a high-priced Hwangchil resin solution (e.g. tens of thousands of won per kg) obtained by hurting the hwangchil wood in the conventional method Compared to the method, it is possible to universally utilize the active ingredient of Hwangchil wood as an food additive.

Hwangchil extract prepared or extracted as described above can satisfy both the aroma effect as a benzoin and the pharmacological effect as a medicinal herb so as to contain an effective hwangchil component and be widely used as a food additive for food. Can be.

According to the data presented in Gangseo civilian herbal herb, 'Gilberry root, Ho Jang-geun, Hong Chong-keun root (western tree root), Balyeo-geun (cheongmirae vine root) 600g each,木 通: Crushed vine stem) Soaked in 3600 grams of soju and left for 7 days, it becomes a custom wine (風濕 酒). It is taken one teaspoon per day. 'Patent publication 10-2003-0075999 (Hwangchil extract, Hwangchil fraction and pharmaceutical composition containing them), which has the effect of hard tissue regeneration and proliferation, Hard tissues are largely classified into bones and teeth, and typical diseases caused by problems with such hard tissues include osteoporosis and periodontal disease, where osteoporosis is caused by a decrease in the total bone mass and the formation of holes in the bones. Bones that break easily or bend, which are commonly found in older people. The most common one is due to the reduction of estrogen after menopause in women. On the other hand, periodontal disease is caused by bacterial inflammation and loss of gingival tissue and alveolar bone. This is a disease that results in tooth extraction as a result ... ideal healing of periodontal tissue In order to repair the periodontal ligament, which connects the tooth and the alveolar bone, surgical procedure is required The most important thing in the healing process is the proliferation of osteoblasts with the preferential attachment of the periodontal ligament to the tooth surface… Hwangchil extract having a hard tissue regeneration-promoting effect according to the present invention, as well as promoting the proliferation of osteoblasts and periodontal ligament cells and increasing the alkaline phosphatase activity of the cells to exhibit hard tissue regeneration and proliferation effect, hard tissue having a sufficient hardness Therefore, it can be used as a direct or adjuvant treatment for various diseases related to hard tissue regeneration or reduction of hard tissue proliferation, and applicable diseases include osteoporosis and periodontal disease. . " Efficacy of bones of high yellow lacquer is described.

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A study on the physiological activity of the specialty yellow lacquer (Baekwoonbong p37, p42 ~ 43) states that “Jilchil extract significantly promoted the proliferation of the periodontal ligament (connecting teeth and alveolar bone) (compared with the control without the yellow lacquer). 3 days on day 14), promoting the differentiation of periodontal ligament cells into osteoblasts, which promoted bone regeneration, and increased the bone strength by promoting the deposition of calcium on the bone surface. ” Studies on the Separation and Analysis of Hwangchil Ingredients (Kim, Han-Han, Hanbatdae, 2003, p13) stated, It is an effective ingredient for rheumatoid arthritis …… germacrene D is used for neuralgia, dysmenorrhea, headache, hematuria and hemostatics. ” Efficacy of rheumatism and arthritis of high yellow lacquer is described.

In addition, the Journal of Human & Experimental Toxicology (August 17, 2010) published an article on overseas health and experiments on the efficacy and effect of Hwangchil-tree, which helps clear blood and improve blood circulation, and helps with diabetes, hypertension, and blood circulation disorders. The study on the anti-diabetic effect of the yellow lacquer extract extracted from the yellow lacquer leaves in normal rats and diabetic rats showed that the effect of the yellow lacquer extract from the yellow lacquer leaf significantly lowered the blood sugar levels of the diabetic rats. Significantly reduced blood sugar and total cholesterol, triglycerides (triglycerides), urea, uric acid, creatinine (white crystals in the blood), AST, and ALT (both enzymes related to liver inflammation), while insulin (pancreas) And glycemic control), but not in normal rats. We compared the anti-diabetic effects of the hwangchil component more effectively than those observed with glybenclamide. ” For this paper, Dr. Sung-Won Kim, Ph.D., Seoul National University's advisory board, said, “The effect of Hwangchil-tree is more effective than the existing anti-diabetic drug (glybencamide) and the amount of input is increased. Glucose levels were statistically significant, and hypoglycemia did not occur even when administered to normal subjects, lowered blood sugar levels, promoted insulin secretion, lowered triglycerides levels, and normalized cholesterol levels. It is not only effective for diabetes, but also shows the possibility of developing new drugs, and because it is a herbal ingredient, it is a very meaningful result compared to the side effects caused by chemical synthetic drugs. Ginseng also did not show this result.

In addition, the overseas medical journal Pharmazie (2009. 8, 64 (8): 547-9) used hwangchil wood as a traditional medicine for various diseases in Korea, and found the efficacy of hwangchil in reducing blood lipids in rat experiments. Decreases total cholesterol, triglycerides (triglycerides; one of the blood fats that cause arteriosclerosis along with cholesterol), and low density cholesterol (LDL), which causes hyperlipidemia. Good cholesterol levels have been increased, and Hwangchil has been shown to be a promising material that has a significant lowering effect on blood lipids and should be considered when researching new, safe and effective natural heart protectants. In the <Anti-hyperlipidemic Activity of Hwangchil Essential Oil Components> (2009 Symposium of the Korean Society of Medicinal Crop Science) Dose proportionally decreases blood levels of total cholesterol low density cholesterol (LDL; bad cholesterol for hyperlipidemia), triglycerides (triglycerides; one of the components of blood fat that causes arteriosclerosis along with cholesterol), and Cholesterol condensation is associated with the risk of coronary artery disease, and the fact that yellowning significantly reduces total cholesterol, low-density cholesterol and triglyceride levels in animal experiments suggests that yellowing is a promising inhibitor of coronary artery disease. It has been shown to significantly increase cholesterol (HDL), which is a useful material for preventing atherosclerosis and managing coronary artery disease.

In addition, the Hwangchil-tree component has the functions of recovering from fatigue and hangover by improving liver function, and in <Physical Activity of Hwangchil, a special product of Korea> (Baikwoonbong, Kyung Hee University, 2003, p31, p37), "GOT (GPT and Together, these enzymes in hepatocytes were markedly reduced in the case of hepatic cell inflammation, and in the case of GPT, especially in the yellow-sea hexane fraction (separated from hexane as hexane). In the case of administration of GOT and GPT, both GOT and GPT were reduced to the normal value, indicating that the effect of protecting liver damage by ethanol (ethyl alcohol, the main ingredient of alcohol) was very excellent. As a result, when the value is high, the liver function, bone, and other diseases are suspected. Administration of Hwangchil extract and its fractions and measurement of the concentration of MDA (malondialdehyde: a type of harmful lipid peroxide) in hepatocytes showed a significant decrease in MDA, especially in the Bacterial butanol and chloroform fractions The separation of chloroform) inhibits the formation of more than 50% of lipid peroxide (unsaturated fatty acid absorbs oxygen and causes aging and various diseases) and shows hepatoprotective effect. " In the Patent Publication 10-2003-0079205 (hwangchil extract having a hepatocellular protective effect, a hwangchil fraction and a pharmaceutical composition containing them), as described above, the hwangchil extract of the present invention or a fraction thereof has an excellent hepatocyte protective effect Therefore, the hwangchil extract of the present invention or a fraction thereof and the pharmaceutical composition comprising the same Since it has no effect, it has an excellent hepatocyte protective effect, so that it can be used in place of or in combination with existing therapeutic agents for the purpose of preventing and treating diseases related to hepatocyte protection such as fatty liver, hepatitis, cirrhosis, etc. Hwangchil extract of the present invention and its fractions can also be used as a medicine, a beverage or a functional food for protecting the liver. ”

In addition, the hwangchil wood component has the function of inhibiting aging and cell damage by the antioxidant activity, which is described in "Study on the Antioxidant Functionality of Hwangchil Tree Extract" (Chang Chang-gon, p13, p17) The extracts have the ability to remove 85% and 75% of free radicals (harmful oxygen causing aging and cell damage), respectively, and Published Patent Application No. 10-2004-0107852 (Hwangchil extract preparation method having skin whitening effect and According to Hwangchil Extract), "the skin whitening effect of inhibiting the activity of tyrosinase forming melanin blackening the skin, kojic acid (kojic acid: representative whitening material used since the 1980s) 10μg / ml and 100μg 15% and 65% melanogenesis was inhibited at 35 ml / ml, while the yellowing fraction showed 35% and 98% melanin inhibition, respectively, with a superior whitening effect than kojic acid. Is there. "

In addition, the hwangchil wood component is effective in bone and teeth rejuvenation, it helps to osteoporosis, periodontal disease, arthritis, which is disclosed in Korean Patent Publication No. 10-2003-0075999 In the pharmaceutical composition), "hard tissue in the human body is largely classified into bones and teeth. Representative diseases caused by problems with such hard tissues include osteoporosis and periodontal disease. It is a disease that causes bones to break or bend easily due to a weak impact due to a decrease in the formation of holes in the bones, which are commonly found in the elderly, and most commonly due to a decrease in estrogen after menopause in women. Inflammation causes loss of gingival tissue and alveolar bone, resulting in teeth The ideal healing of periodontal tissue requires remodeling of the periodontal ligament that connects the teeth and alveolar bone, and surgical procedure is required. Hwangchil extract having a hard tissue regeneration promoting effect or a fraction thereof according to the present invention promotes the proliferation of osteoblasts and periodontal ligament cells and increases the alkaline phosphatase activity of the cells to increase the hard tissue regeneration and proliferation effect. In addition, it can be used as a direct or adjuvant treatment for various diseases related to hard tissue regeneration or reduction of hard tissue proliferation. And osteoporosis, periodontal disease, etc. " The study on the physiological activity of Korean special products, Hwangchil (Baik Woonbong; p37, p42 ~ 43), said, 3 days compared to the non-control group), promoting the differentiation of the periodontal ligament cells into osteoblasts showed an effect of promoting bone regeneration, and increased the bone strength by promoting the adhesion of calcium to the bone surface. " It explains.

In addition, the hwangchil wood components have the effect of strengthening immunity, about this, the overseas medical journal <J Ethnopharmacol. 2004 Feb; 90 (2-3: 403-8> "Hwangchil is used in various diseases such as migraines and menstrual disorders in Korea, and it separates six known components and one new polyacetylline component from Hwangchil. It has been shown to have anti-complement (related to biodefense / immunity) activity, and the newly isolated polyacetylline component may be particularly important anti-complement activity. " Exploration of Biologically Active Substances> (Chonnam National University Research Report (published by the Ministry of Agriculture and Forestry) 2003, p63, p82), "Promoting growth in immunostimulation experiments using human immune cells (T and B cells) of Hwangchil extract showed both T and B cells. … 1.27-fold and 1.21-fold growth-promoting activity… IL-6 (interleukin-6: immunostimulatory cytokines in blood) was found in T cells and B cells… 73.8 (pg / ml) and 64.8 (pg / ml ) In addition, TNF-α (immune-promoting cancer necrosis factor) also showed significant enhancement effects in B cells and T cells… 121 (pg / ml) and 102 (pg / ml) …… (Hwangchil extract In the immunoassay, the activity was higher than the reference substance retinoic acid (retinoic acid, the most well-known and well-known anti-cancer / immune drug), and the same result was obtained in the growth of immune cells. This suggests that (another useful) substance, which is not included in retinoic acid, which is a pure substance, is contained in the extract of yellow lacquer. " Lee Seo-Ho et al., Korean Society for Biotechnology and Biotechnology Trend X: 2002.4 p551), said, “Hwangchil… has a high value as a herbal medicine, among which has the characteristics of maintaining the homeostasis of living organisms, In particular, this study has significance in assigning new value using leaves, not bark or root, in order to add new value as a medicinal plant of Hwangchil-tree. "Increasing growth, growth and cytokines (IL-6, TNF-α) of human B and T cells play an important role."

In addition, the hwangchil wood component has a neurostable effect, which is described in the Jeonnam University Research Report (Ministry of Science and Technology) <Diversification of Hwangchil tree resource utilization technology and utilization methods: 1st year, p110> It has a unique fragrance, which stabilizes people's nerves, so it is essential for mental hygiene. ”The herbaceous tree (Lee Si-jin, Chinese Ming Dynasty) said," This scent defeats musical instruments and calms all morale. Because it is called benzoin. "

In addition, the hwangchil wood components have antibacterial and anti-inflammatory effects, and the <Hwangchil components from hwangchil through chloroquinone susceptible protozoa> (Jil Il Min et al., 2009, overseas journal <Phytoher Res. 2009 4.14> Quinone (synthetic antimalarial agent) has been investigated as having antimalarial efficacy against susceptible tropical pyrophytes (human malaria species), and the Jeonnam National University Report (Published by the Ministry of Science and Technology; Diversification of Hwangchil-tree resource utilization technology and utilization 2) In the next year, p156), "Anti-microbial activity was recognized against bacteria in terms of pathogens or food rot." The Chosun Ilbo, April 10, 2009, quoted the findings from the Jeonnam Institute of Health and Environment. "The effect of inhibiting the growth of staphylococcus aureus, Clostridium perfringens, Vibrio bacteria that cause food poisoning has been confirmed." And says.

In addition, the hwangchil wood component has an anticancer effect, and in this regard, the Chonnam National University research report (published by the Ministry of Agriculture, <Stable production technology development of Hwangchil and the search for bioactive substances of the origin of hwangchil wood> p63 ~ 65, p81), "Hep 3B ( The growth inhibition rate of liver cancer cells), MCF7 (breast cancer cells), A549 (lung cancer cells) and AGS (gastric cancer cells) were 73, 63, 69 and 71%, respectively, and selectivity (selectivity: growth against cancer cells) was high. Inhibitory activity / toxicity to normal cells) was also high in liver cancer cells 5.5 and breast cancer cells 4.3. "Patent Publication 10-2006-0131360 (seed extract of Hwangchil-tree with high physiological activity) showed" HL-60 and The proliferation inhibitory effect was prominent in all Jurkat cells (leukemia cells), and morphological observations of leukemia cells treated with yellow lacquer showed that the cells were reduced in size, irregular in shape of the nucleus, and partial nucleation of the nucleus. prefecture It had to be observed, which cell death: it was confirmed that (Apoptosis is the mechanism that cells destroy themselves) tumor growth inhibition induced by, "he explains.

On the other hand, about the components of Hwangchil, <The Study on the Separation and Analysis of Hwangchil Components> (Cho Yong-hwan (Hanbatdae), 2003, p13), the composition and efficacy have been found as follows.

A. alpha-cubebene

-Effective against epilepsy, neuropathy, insomnia, hysteria, migraine, dizziness.

-In addition to diseases of the small intestine such as stomach pain and flatulence, it also works on the digestive system and is used for hyperthermia and blood circulation.

B. β-elemene

-Controls diuretics, headache relief, bed-wetting.

-It stimulates the central nervous system and hormonal system, which is good for the male hormone system and also used to treat depression.

C. alpha-muurolene

-It is effective for digestive diseases, tonics, antiperspirants, emollients and sedatives, and rheumatoid arthritis.

D. germacrene D

-Neuralgia, dysmenorrhea, headache, hematuria and hemostatic use.

E. Beta-sitosterol

-Prostatic hyperplasia (<cytonyl>) used as a therapeutic agent for the treatment of enlarged prostate gland, prostatitis, neuroprostate disease, bladder dysfunction (urination disorder, nocturnal enuresis, urinary incontinence, anemia, etc.) before and after prostate surgery.

In addition, various media have been introduced to the nerve stabilizing effect of hwangchil trees, for example, in the quote quoted by President Han Hyung-hee of <153 World Medical Clinic> in eDaily News (October 26, 2009), Balancing nerve autonomic hormones: Hwangchil is excellent for balancing broken brains and catching fever caused by excessive energy consumption, and toxic substances released from nerves, blood, waste products, free radicals, inflammation, and soaking. It has been regarded as the best in Korea since ancient times as an excellent medicine that quickly removes unnecessary elements and balances the brain and books. " MBC TV <6 o'clock News Magazine> Dr. Kim Soo-beom, a doctor of Korean medicine (November 18, 2010), said, “Hwang Chil-tree is good for people with low limbs or pain in their limbs and blood circulation disorders or menstrual disorders. It is also excellent for the recovery of liver function. "

On the other hand, in the method of extracting the hwangchil wood extract according to the present invention includes a step of mixing the vinegar, sugar components, water to the crushed hwangchil wood raw material, the effect of the added vinegar is as follows.

First of all, Langzhou, the city of Chinese vinegar, has a long tradition of saying, "Eat less salt and eat more vinegar for health." And Professor Lee, Deok-hwan of Sogang University, said, "We have developed a unique technology that converts glucose into alcohol using a microorganism called yeast. An acetic acid that produces a sour taste can be made by adding acetobacter bacteria to such alcohol. Our favorite 'natural' liquor and vinegar are actually by-products of bacteria. " He admired that the vinegar was born from the amazing chemical knowledge of mankind.

Vinegar is the best chemical produced by mankind. Vinegar is the only substance produced through acetic acid effectiveness. Vinegar has a tremendous power hidden in it, and it is a renewable material that is being studied in depth to find out. He has won three Nobel Prizes in Physiology for vinegar. The vinegar produced by acetic acid effectiveness has sterilization power to sterilize to super bacteria and oxidation power to oxidize copper or iron. Both of these functions have a surprising impact on humanity and the environment. The efficacy of vinegar comes from the first two functions, the ability of which is arranged as follows.

1. The ability to remove radioactive pollutants

Dr. Gil-Soon Ko, Ph.D. Professor of Medicine at Seoul National University, said, "Vinegar is directly involved in human metabolism, and vinegar is directly involved in human metabolism. It plays an important role in promoting absorption, so it can be used in medicine.

The most prominent of these is by far the removal of radioactive contaminants. In general, there are no known medicines that can remove radiation exposure or radioactive contaminants that enter the body and accumulate in cells or muscles. Therefore, the fact that among the efficacy of natural vinegar is the ability to remove radioactive material has great significance.

Dr. Gil-Soon Shim's theory of radioactive contaminant removal and Dr. F. Lippman's sulfoamide elimination theory at the Institute of Biochemistry at the Massachusetts General Hospital, Nobel Prize in Physiology, say the same. "The acetic acid combines toxic substances with other molecules to form new compounds," said Lifman. "Sulfonamide, when combined with acetate, makes it biologically inactive and produces compounds that are easy to excrete in vitro." It creates new compounds for toxic substances that cleanse the inside and outside of the body. ” Dr. Gil-Soon Shim and Dr. Ripman's theory conclude that 'the radioactive material introduced into the body is combined with acetate, which is easily removed by drinking vinegar because it is a compound that is easy to excrete in vitro.'

2. Ability to remove carcinogens

Carcinogen, which is considered to cause cancer, is a chemical that causes cancer in the living body. Physical factors such as radioactive substances, biological factors such as viruses, hormones, etc. also mean carcinogens in a broad sense, but generally refer to substances causing cancer by chemical stimulation. Many chemical carcinogenesis such as aromatic hydrocarbons such as methyl cholanster, benzopyrene and dibenzoanthracene, azo dyes such as orthoamide azotoluene and butter yellow, aromatic amines such as naphthylamine, alkylating agents such as nitroquinoline and nitrogen mustard, and nitroso compounds The substance is being discovered.

The accumulation of chemical carcinogens in the body is the first cause of cancer. Acetic acid bacteria of natural vinegar are capable of synthesizing chemical toxic substances such as sulfonamides, which are accumulated in the body, into compounds with different traits and excreting them out of the body. Sulfonamide is a sulfaicide, an antibiotic, a source of saccharin, and used as a dye. In addition, primary carcinogenic heavy metals, such as dioxins and cadmium, are simply oxidized and released into the body. There is no drug that can break down and release toxic substances accumulated in the body on Earth. Only natural vinegar has the power. Therefore, there is a need to drink natural vinegar to prevent the occurrence of one-dimensional cancer and two-dimensional cancer.

Humans have been fighting cancer until the present day. Numerous investments and studies have been made to conquer cancer and have developed surgical, antibiotic and radiotherapy. In addition, for example, the development of alternative therapies combining physical fitness has been tried in various ways. As far as cancer is concerned, there is no separate crystal.

In recent years, the concept of a five-year cure is breaking down due to two-dimensional cancer. In the past, surviving five years after cancer treatment was considered a cure. However, the concept of 5-year cure must be reserved because the proportion of 2D cancer that occurs in 5 to 10 years is on the rise.

Many approaches have been taken to determine the identity of cancer, but the identity of the cancer, which may be a clue to a breakthrough treatment, is still heavy. Perhaps the approach to finding groundbreaking treatments will eventually fail. This is because cancer is a naturally occurring disease.

3. Ability to remove antibiotic toxic substances

Antibiotic toxicity is the main culprit that accumulates in the body and weakens immunity. When antibiotics accumulate in the body, they become toxic and cause rejection of the same antibiotics. To specify this as an antibody of antibiotics, to be precise, it means it is toxic.

For the first time, Dr. Ripman found that acetic acid in vinegar eliminates the toxicity of antibiotics. Sulfoamide, which is a raw material of saccharin and dye, is used as a sulfa agent and has high chemical and coloring affinity. Sulfonamide-based antibiotics, which are sulfa drugs, have very high accumulation rates and side effects and are extremely limited. Dr. Lifman, along with Dr. Crebbs of England, co-winned the Nobel Prize in Physiology or Medicine for his research on the effects of acetic acid on humans.

4. Detoxification ability

Natural vinegar is detoxifying. Not long ago, according to Australian journalist Townsville News, a 10-year-old girl shot by a box jellyfish was temporarily blinded by the poison of the jellyfish, and when she could not breathe properly, a camper's vinegar was poured into the girl's legs. Here is an example of how he detoxified his life and saved his life.

Vinegar's ability to neutralize and detox jellyfish's deadly poison is due to the oxidative ability of the acetobacter bacteria.

5. Pesticide removal ability

Soak vegetables or fruits in a vinegar diluted 1:10 of natural vinegar for 30 minutes to completely remove residual pesticides. This is due to the oxidative power of acetobacter bacteria of natural vinegar. Because pesticides are formulated with chemicals, they are decomposed by the oxidative power of acetobacter bacteria, which transforms and removes them.

Acetobacter bacteria, which produce acetic acid by secondary fermentation of alcohol, have the ability to break down alcohol to less than molecular units and completely change its traits into acetic acid. The alcohol decomposed to the molecular unit or less forms various nutrients in the process of dissociation and recombination as the raw rice component is decomposed to the molecular unit or less. More than 60 kinds of rice vinegar made from white rice and more than 80 kinds of nutrients are contained in black vinegar made from brown rice. This exceptional decomposition ability is due to the oxidative power of acetobacter bacteria. Acetobacter bacteria are aerobic bacteria that inhale oxygen and exhale carbon dioxide and water.

When an object is put into the active area of acetobacter living bacteria, the acetobacter bacteria inhales oxygen, decomposes the object, and releases carbon dioxide and water. From the oxidation of heavy metals and nonferrous metals, to the oxidation of toxic substances in antibiotics, to the oxidation of radioactive substances, to the oxidation of pesticides, it works specifically to oxidize and sterilize fungi, including decayed bacteria.

All toxins and bacteria that enter the active zone of acetobacter bacteria are degraded or disinfected within 30 minutes. No matter how toxic and viable microorganisms are, there are no microorganisms on the planet that can cope with live acetobacter bacteria. Acetic acid bacteria of vinegar completed by acetobacter bacteria retain all the properties of acetobacter bacteria.

Vinegar, which has both oxidizing and bactericidal powers, reacts differently depending on each application or at the same time and completely removes harmful substances to benefit mankind. The quality of life can be improved by the ability of vinegar to show oxidizing power in applications requiring oxidizing power and sterilization in applications requiring sterilizing power. In addition, vinegar is acidic on the surface, but it is a mysterious substance that retains the alkalinity of alcohol.

Therefore, you can eat food contaminated with radioactive materials or pesticides if you eat it after dipping all ingredients, including meat, fish, vegetables, and fruits, for 30 minutes in water diluted 10 times with natural vinegar.

In addition, the efficacy of the sugar component used in the extract of the Hwangchil wood extract according to the present invention serves to provide a sugar component to the yeast. That is, the sugar component serves as an additive for fermentation, and also serves to reduce the bitter taste.

Although the present invention has been described with reference to the embodiments illustrated in the accompanying drawings, it is merely an example, and a person having ordinary knowledge in the art related to the present invention may have various modifications or equivalents thereto. I will understand that it is possible.

Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (3)

Washing hwangchil wood with water, washing step to remove dirt, dust, foreign matter (S11);
Sacred tree crushing step (S12) for making the sulfur wood wood into a pulverized raw material of 0.05cm ~ 3cm size;
Adding a vinegar, a sugar component, and water to agitate the ground raw material for 20 to 40 minutes in a mixed liquid (S14);
Removing the mixed liquid used for the acupuncture and leaving only the ground raw material (S15);
Removing the mixed liquid and putting the remaining raw material, which was then pulverized, into the Onggi jar soaked in water to ferment at 15 ° C. to 25 ° C. for 7 days to 10 days (S16);
Putting the fermented broth fermented with the raw materials and water after the grinding into an extractor and extracting at low temperature at 55 ° C. to 65 ° C. (S17); And
Concentrating the extracted extract at low temperature between 60 ℃ ~ 70 ℃ (S18)
Hwangchil wood extract extraction method comprising a.
The method of claim 1,
The acupuncture step (S14) is to immerse the pulverized raw material for 28 minutes to 32 minutes to the liquid mixed by adding the vinegar, sugar components and water,
The fermentation step (S16) is to put the raw material pulverized after the grinding soaked in water in the Onggi jar 7 to 10 days at 17 ℃ ~ 19 ℃ fermentation,
The low temperature extraction step (S17) is put into the extractor fermentation broth fermented with the raw material and water pulverized after the grinding, characterized in that the extraction at 58 ℃ ~ 62 ℃
Hwangchil wood extract extraction method.
The method of claim 1,
After the low temperature concentration step,
Hwangchil wood extract extraction method further comprises the step of product packaging the concentrated extract (S19).

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