KR20200025834A - Red sea cucumber-derived low salt peptide powder and process for producing the same - Google Patents

Abstract

The present invention relates to a red sea cucumber-derived peptide having a reduced salt content and a method for producing the same. By a producing method according to the present invention, red sea cucumber-derived peptide powder is produced by optimizing a desalting condition, thereby having an effect of producing high purity red sea cucumber peptide having low salt concentration which can be used as a cosmetic raw material.

Description

Red sea cucumber-derived low salt peptide powder and process for producing the same

The present invention relates to a peptide powder derived from red sea ginseng with a reduced salt content and a method for preparing the same.

Sea cucumber is a general term of sea cucumber belonging to the echinoderm sea cucumber river, and there are more than 1,500 species in the world, and 14 species are known to live in Korea, and the high commercial value is Apostichopus japonicas species. This species is classified as red sea ginseng, blue sea ginseng and black sea ginseng according to color classification. Recent studies have revealed genetic diversity, and red sea ginseng has the best taste, aroma and size. Sea cucumber has the highest protein content with 47.43% carbohydrate 16.67% and 35.90% ash, and various studies have been conducted to use it as a food or beauty ingredient because it is rich in various nutrients.

Chondroitin of sea cucumber cartilage acts to increase elasticity by allowing fluid to stay in the cartilage to nourish the cartilage of joints and absorb physical shocks and stresses. Prevents discomfort caused by stress, stiff shoulders, headaches and other physical and mental disorders and skin aging and neutralizes poisoning. It can also be used for sexual dysfunction, constipation, gastric ulceration, tooth and skeletal formation, muscle paralysis, and excessive bleeding.

Fucose sugar-containing chondroitin sulfate in sea cucumber not only induces platelet aggregation, but gangriosides are also known to be active in neurogenesis. In addition, in the biological action on animal cells, fucose sugar-containing chondroitin sulfate inhibits sperm-egg interaction, inhibits viral infection, inhibits cell-cell binding mediated by P-selectin or L-selectin, anti- It is known to promote coagulation and inhibit the proliferation of fibroblasts.

In addition, fucose sugar-containing chondroitin sulfate has recently been known to strongly inhibit the bone destruction of osteoclasts.

On the other hand, the present inventors while studying red sea ginseng as a cosmetic raw material, it was confirmed that the formulation of the cosmetic formulation is unstable because a large amount of salt is contained when preparing the peptide powder from the red sea ginseng. Accordingly, the red sea ginseng-derived peptide manufacturing method with reduced salt content was developed, and the present invention was completed by checking the optimum freshwater temperature conditions.

Korea Patent Registration 10-1734331

It is an object of the present invention to provide a method for preparing red sea ginseng-derived peptide powder.

Another object of the present invention to provide a red sea ginseng-derived peptide powder prepared according to the present invention.

Still another object of the present invention is to provide a cosmetic composition comprising the red sea ginseng-derived peptide powder.

In order to achieve the above object,

In the present invention, red sea ginseng is put in fresh water at 15 to 25 ° C. for 2 to 4 hours. Storage to remove primary salts (step 1);

Removing red sea ginseng from the primary salt of step 1 removed and cutting the gut and washing it, and putting it in fresh water at 5 to 18 ° C. for 10 minutes to 1 hour to remove the secondary salt (step 2);

Fixing red sea ginseng from which the second salt of step 2 is removed in 95-105 ° C. boiling water to which 0.01% to 0.5% of vinegar is added for 1 to 3 minutes to fix the form (step 3);

Drying red sea ginseng fixed in the form of step 3 at 40 to 80 ° C. for 12 to 72 hours (step 4);

Grinding the dried red sea ginseng of step 4 into 20 to 100 mesh (step 5);

Mixing 50 to 1000 parts by weight of purified water to 50 parts by weight of the ground red sea ginseng powder of step 5 and then ripening at 80 to 140 ° C. for 5 to 30 minutes (step 6);

Inoculate 10 ⁴ CFU / ml to 10 ³ CFU / ml of the microorganism containing protease or peptidase in the red sea ginseng cooked product of step 6 and stirring it at 80 to 250 rpm at 30 to 40 ° C. for 24 to 72 hours. After the first fermentation, the step of killing the bacteria using a high-pressure sterilizer (step 7);

Proteolytic enzyme is mixed with the red sea ginseng fermentation product of step 7 in an amount of 0.01 to 1.5 parts by weight based on 1 part by weight of red sea ginseng powder of step 5, followed by secondary fermentation at 50 to 70 ° C. for 3 to 24 hours, and then 10 to 80 to 90 ° C. Inactivating the enzyme for 30 minutes (step 8); And

Centrifugation and filtration at 2,500 to 3,500rpm after the second fermentation of step 8, concentrating and lyophilizing to prepare a powder (step 9);

It provides a method for producing red sea ginseng-derived peptide powder comprising a.

The present invention also provides a red sea ginseng-derived peptide powder prepared according to the above method.

Furthermore, the present invention provides a cosmetic composition comprising the red sea ginseng-derived peptide powder.

The preparation method according to the present invention has the effect of optimizing the desalting conditions to produce red sea ginseng-derived peptide powder, thereby producing a high purity red sea ginseng peptide of low salt concentration which can be used as a cosmetic raw material.

1 is a manufacturing process of the red sea ginseng-derived peptide powder reduced salt content of the present invention.

Hereinafter, the present invention will be described in detail.

Manufacturing method

In the present invention, red sea ginseng is put in fresh water at 15 to 25 ° C. for 2 to 4 hours. Storage to remove primary salts (step 1);

Removing red sea ginseng from the primary salt of step 1 removed and cutting the gut and washing it, and putting it in fresh water at 5 to 18 ° C. for 10 minutes to 1 hour to remove the secondary salt (step 2);

Fixing red sea ginseng from which the second salt of step 2 is removed in 95-105 ° C. boiling water to which 0.01% to 0.5% of vinegar is added for 1 to 3 minutes to fix the form (step 3);

Drying red sea ginseng fixed in the form of step 3 at 40 to 80 ° C. for 12 to 72 hours (step 4);

Grinding the dried red sea ginseng of step 4 into 20 to 100 mesh (step 5);

Mixing 50 to 1000 parts by weight of purified water to 50 parts by weight of the ground red sea ginseng powder of step 5 and then ripening at 80 to 140 ° C. for 5 to 30 minutes (step 6);

Inoculate 10 ⁴ CFU / ml to 10 ³ CFU / ml of the microorganism containing protease or peptidase in the red sea ginseng cooked product of step 6 and stirring it at 80 to 250 rpm at 30 to 40 ° C. for 24 to 72 hours. After the first fermentation, the step of killing the bacteria using a high-pressure sterilizer (step 7);

Proteolytic enzyme is mixed with the red sea ginseng fermentation product of step 7 in an amount of 0.01 to 1.5 parts by weight based on 1 part by weight of red sea ginseng powder of step 5, followed by secondary fermentation at 50 to 70 ° C. for 3 to 24 hours, and then 10 to 80 to 90 ° C. Inactivating the enzyme for 30 minutes (step 8); And

Centrifugation and filtration at 2,500 to 3,500rpm after the second fermentation of step 8, concentrating and lyophilizing to prepare a powder (step 9);

It provides a method for producing red sea ginseng-derived peptide powder comprising a.

In the manufacturing method according to the present invention, before the step 1 may further include the step of removing the foreign material in the red sea ginseng, preferably, remove the foreign material by storing the red sea ginseng in the net so as not to be exposed to the water surface for 24 hours It may be.

In the manufacturing method according to the present invention, step 1 is a step of removing the primary salt (primary desalting) by putting red sea ginseng in fresh water at 15 to 25 ℃ for 2 to 4 hours. Preferably, the red sea ginseng may be added to fresh water at 15 to 21 ° C. to remove salts, and more preferably, the red sea ginseng may be added to fresh water at 17 to 19 ° C. to remove salts.

In the manufacturing method according to the present invention, the step 2 is removed by removing the intestines of the red sea ginseng in which the primary salt of step 1 is removed, washed with water, put in fresh water at 5 to 18 ℃ and stored for 10 minutes to 1 hour 2 The secondary salt is removed (secondary desalting). Preferably, red sea ginseng may be added to remove fresh salt from 11 to 17 ° C. fresh water, and more preferably, red sea ginseng may be added to fresh water at 13 to 15 ° C. to remove salts.

In the manufacturing method according to the present invention, the step 3 is a step of fixing the deodorization and form of the red sea ginseng with the secondary salt of step 2 removed in boiling water added with vinegar. Using vinegar is a process for fixing and deodorizing the form, and the vinegar may use various vinegars prepared through fermentation such as fruit and grain fermentation vinegar. Preferably, vinegar fermented with fruit or cereals may be used, and more preferably, vinegar fermented with fruit may be used.

In the manufacturing method according to the present invention, step 4 is a step of drying the red sea ginseng fixed in the form of step 3 at 40 to 80 ℃ for 12 to 72 hours. Preferably it can dry for 20 to 30 hours at 60-80 degreeC, More preferably, it can dry for 24 hours at 70 degreeC.

In the manufacturing method according to the present invention, step 5 is a step of grinding the dried red sea ginseng of step 4 to 20 to 100 mesh size.

In the manufacturing method according to the present invention, step 6 is a step of ripening for 5 to 30 minutes at 80 to 140 ℃ after mixing purified water with 800 to 1000 parts by weight to 50 parts by weight of red sea ginseng powder of the step 5. Preferably, the cooking temperature may be 10-20 minutes at 115 to 125 ℃, and more preferably 15 to 20 minutes at 120 to 125 ℃. If the cooking temperature is less than 100 ℃ or more than 140 ℃ there may be a problem that the content of protein dissolved in purified water is lowered, and if the cooking time is less than 5 minutes or more than 30, the content of protein dissolved in purified water is low Losing can be a problem.

The red sea ginseng-derived protein may be dissolved in purified water at a high rate in the ripening conditions according to the present invention, and the more the protein is dissolved, the higher the content of the peptide is produced compared to the total protein content in the subsequent fermentation (hydrolysis) step.

In the manufacturing method according to the present invention, the step 7 is inoculated with microorganisms in the red sea ginseng self-contained in step 6 and the first fermentation for 24 to 72 hours while stirring at 30 to 40 ℃ 80 to 250rpm, autoclave This step is to kill the bacteria. The microorganism of step 4 is preferably a microorganism including a protease or a peptidase, and inoculates 10 ⁴ CFU / ml to 10 ³ CFU / ml with respect to 1 ml of red sea ginseng cooked water, more preferably. It can be inoculated with 10 ⁴ CFU / ml compared to 1 ml of red sea ginseng cooked water.

In addition, the microorganism including the protease or peptidase is preferably a Bacillus genus microorganism, and more preferably Bacillus subtilis .

In the manufacturing method according to the present invention, the step 8 is 50 to 3 to 24 hours by mixing the proteolytic enzyme in 0.01 to 1.5 parts by weight to 1 part by weight of the red sea ginseng powder of the step 5 to the red sea ginseng primary fermentation of the step 7 It is a step of inactivating the enzyme for 10 to 30 minutes at 80 to 90 ℃ after the secondary fermentation (enzyme hydrolysis) at ~ 70 ℃.

The proteolytic enzyme of step 8 may be preferably mixed with 0.5 parts by weight of protease relative to 1 part by weight of red sea ginseng powder of step 5, and the proteolytic enzyme may be used alone or in combination with metallopeptidase, metalloprotease, or the like. It may be used, and preferably, thymolysin (Thermolysin: microorganism derived protease produced by EC 3.4.24.27.Bacillus thermoproteolyticus) may be used. In addition, the step of inactivation may preferably deactivate the enzyme for 20 minutes at 90 ℃.

In the manufacturing method according to the present invention, step 9 is centrifugation and filtration at 2,500 to 3,500rpm after the second fermentation of step 8 to remove the residue, and then concentrated and lyophilized to prepare a powder. Preferably, after the second fermentation of step 8 may be centrifuged at 3,000rpm.

Red sea ginseng-derived peptides prepared according to the method of the present invention are characterized in that the salinity is significantly lower than the red sea ginseng-derived peptides prepared according to the same method except that the salt removal (desalting) process of steps 1 and 2 is omitted. do.

Peptide Powder from Red Sea Ginseng

The present invention provides a red sea ginseng-derived peptide powder prepared according to the above method.

Red sea ginseng-derived peptide powder according to the present invention has an effect that the salt content is significantly reduced.

Cosmetic composition

The present invention provides a cosmetic composition comprising the red sea ginseng-derived peptide powder.

The formulation of the cosmetic composition of the present invention is not particularly limited, but is preferably a formulation of an external preparation for skin, and one preferred form of the external preparation for external preparation is softening cream, nourishing cosmetic, massage cream, nutrition cream, pack, essence, cleanser Cosmetic compositions having formulations of gel or skin adhesive cosmetics, and other preferred forms include transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays. In addition, in the external preparation composition of each formulation, other components than the essential components described above can be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other external preparations.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and / or biologies obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a warm vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or concealed stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of topically or systemically applicable auxiliaries commonly used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventionally used in the art. It may be prepared according to the method.

In addition, the cosmetic composition of the present invention is a fatty material, organic solvent, solubilizer, thickener and gelling agent, softener, antioxidant, suspending agent, stabilizer, blowing agent, fragrance, surfactant, water, ionic or nonionic emulsifier, In the field of cosmetics such as fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics It may contain a commonly used adjuvant. These adjuvants are introduced in amounts generally used in the cosmetic field.

The composition of the present invention can be formulated in the form of a liquid, aerosol, sterile injectable solution, and when formulating the composition, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. that are commonly used Can be used.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely to illustrate the present invention, the contents of the present invention is not limited by the following examples.

< Example  1> Red Sea Ginseng  origin Peptide  Preparation method of powder

Red sea ginseng was collected and put in a net and stored in seawater for 24 hours to remove sand and foreign substances inside the red sea ginseng, and then red sea ginseng-derived peptide powder was prepared through the following steps.

Step 1: Remove red sea ginseng in 15 ~ 21 ℃ fresh water for 3 hours to remove primary salt.

Step 2: Remove the intestines of the red sea ginseng with the primary salt removed, cut and wash once with purified water, and remove the secondary salt by putting it in fresh water at 11-17 ° C for 30 minutes.

Step 3: The red sea ginseng with the second salt removed is added to 100 ° C. boiling water containing 0.1% of vinegar to fix the form.

Step 4: Remove the water of the fixed red sea ginseng and dry for 24 hours at 70 ℃.

Step 5: The dried red sea ginseng is ground to 20 to 100mesh using a grinder.

Step 6: 50 g of ground red sea ginseng powder and 950 ml of purified water are mixed and warmed in a high-pressure (1.5 atm) high temperature container sealed at 120 ° C. for 15 minutes.

Step 7: After cooling the cooked red sea ginseng cooked water (50 g of red sea ginseng + 950 ml of purified water) to 35 ° C., maintaining the temperature, and inoculating Bacillus subtilis to 10 ⁴ CFU / ml per 1 ml of cooked red sea ginseng And then fermented for 48 hours while stirring at 160rpm at 37 ℃, using a high-pressure sterilizer to kill the bacteria to prepare a primary fermentation product.

Step 8: Mixing 0.5 parts by weight of thymolysine (Thermolysin: EC microorganism-derived metalloproteinase produced by Bacillus thermoproteolyticus) to 1 part by weight of red sea ginseng dry powder of step 5 to the primary fermentation product of red sea ginseng Secondary fermentation was carried out by stirring at 150 rpm at 60 ° C. for 4 hours, followed by inactivation of enzymes at 90 ° C. for 20 minutes to produce secondary fermented red sea ginseng. At this time, the red sea ginseng protein is hydrolyzed and peptideized during the secondary fermentation.

Step 9: The red sea ginseng secondary fermentation product is centrifuged and filtered at 3,000 rpm to remove red sea ginseng tissues and foreign substances that are not soluble in purified water. The recovered filtrate is concentrated under reduced pressure and freeze-dried to make peptides derived from red sea ginseng in powder form.

In this case, the red sea ginseng-derived peptide powder of Examples 1-1 to 1-1 was prepared by varying the temperature of fresh water used in the salt removal process of steps 1 and 2 as shown in Table 1 below.

Example 1-1 1-2 1-3 1-4 1-5 1-6 1-7 1-8 1-9 1-10 1-11 1-12 1-13 Freshwater temperature of step 1 (℃) 18 15 16 17 19 20 21 18 18 18 18 18 18 Freshwater temperature of step 2 (° C) 14 14 14 14 14 14 14 11 12 13 15 16 17

< Comparative example  1>

Red sea ginseng-derived peptide powder of Comparative Example 1 was prepared in the same manner except for the salt removal process of steps 1 and 2 of Example 1.

Example 2 Preparation of Red Sea Ginseng Cooked Liquid

The freshwater temperature of step 1 and step 2 of Example 1 was set to 18 ° C. and 14 ° C., respectively, in the same manner as in Steps 1 to 6 of Example 1 to prepare red sea ginseng ripened water, and then filtered. Red sea ginseng cooked liquid of 2 was prepared.

Comparative Example 2

The red sea ginseng cooked liquid of Comparative Example 2 was prepared in the same manner except for the salt removal process of steps 1 and 2 of Example 2.

<Experiment 1> Comparison of salinity according to the salt removal process

The red sea ginseng-derived peptide powder prepared according to Example 1 and Comparative Example 1 was dissolved at a concentration of 1,000 ppm using purified water, and then salinity analysis was performed.

In order to measure the salt content of each peptide powder, chlorine ions are quantitatively reacted with silver nitrate, and excess silver nitrate is reacted with chromic acid to determine the concentration of chlorine ions as the end point of titration. The salt content of the red sea ginseng-derived peptide powder samples of Example 1 and Comparative Example 1 was measured.

50 ml each of red sea ginseng-derived peptide powder samples of Example 1 and Comparative Example 1 was accurately taken into a Erlenmeyer flask, and 1 ml of potassium chromate solution was added and titrated with silver nitrate solution (0.01 N). The end point of the titration was when pale reddish yellow precipitate appeared. Separately, 50 ml of purified water was used as a blank test solution, and the test was performed according to the test method of the sample. The salinity was measured three times in total, and the average value thereof was compared.

Item salt(‰) Sample classification Primary Secondary 3rd Average Example 1-1 1.9 1.9 1.8 1.9 Comparative Example 1 4.2 4.1 4.2 4.1

As a result, the red sea ginseng-derived peptide powder of Example 1-1, which was subjected to the desalting process, contained 1.87 ‰ of salt. Was confirmed. Accordingly, it was confirmed that about 55.2% salt was removed by the salt removal process of steps 2 and 3 of Example 1.

< Experimental Example  2> Comparison of salinity according to freshwater temperature

The salt content of the red sea ginseng-derived peptide powder according to the fresh water temperature used in the salt removal process of steps 1 and 2 of Example 1 was compared. The salt content was analyzed in the same manner as in Experimental Example 1, the measurement results are shown in Table 3.

Item Freshwater temperature salt(‰) Sample classification Freshwater temperature of step 1 (℃) Freshwater temperature of step 1 (℃) Primary Secondary 3rd Average Example 1-1 18 14 1.9 1.9 1.8 1.87 Example 1-2 15 14 3.1 3.0 3.1 3.07 Example 1-3 16 14 2.9 2.9 3.0 2.93 Example 1-4 17 14 2.0 2.1 2.0 2.03 Example 1-5 19 14 2.1 2.0 2.1 2.07 Example 1-6 20 14 2.9 3.0 2.9 2.93 Example 1-7 21 14 3.2 3.1 3.1 3.13 Example 1-8 18 11 3.1 3.2 3.1 3.13 Example 1-9 18 12 3.0 3.0 2.9 2.96 Example 1-10 18 13 2.1 2.0 2.0 2.03 Example 1-11 18 15 2.1 2.1 2.0 2.07 Example 1-12 18 16 3.1 3.1 3.2 3.13 Example 1-13 18 17 3.3 3.2 3.2 3.23 Comparative Example 1 - - 4.2 4.1 4.2 4.17

As a result, as shown in Table 3, when the freshwater temperature of step 1 was 17-19 ° C. and the fresh water temperature of step 2 was 13-15 ° C., the salinity of the red sea ginseng-derived peptide powder was removed. In particular, step 1 It was confirmed that the salt removal effect is the best when the fresh water temperature of 2 and 2 is 18 ℃ and 14 ℃, respectively.

< Experimental Example  3> Comparison of electrical conductivity with and without desalination

The red sea ginseng-derived peptide powder prepared in accordance with Examples 1-1 and 2 and Comparative Examples 1 and 2 and the red sea ginseng cooked liquid were measured for electrical conductivity using an electrical conductivity measuring device which is an analyzer manufactured in mettler toledo.

Item Conductivity measurement value (mS) Sample classification Purified water Red Sea Ginseng Peptide Powder from Red Sea Ginseng Example 2 Comparative Example 2 Example 1-1 Comparative Example 1 Primary 0.351 357 1516 3.83 531 Secondary 0.353 358 1515 3.86 534 3rd 0.350 358 1517 3.88 536 Average 0.35 357.67 1,516.00 3.86 533.67

As a result, when the salt removal process (step 2 and step 3) of red sea ginseng was carried out, the self-contained liquor had an electrical conductivity lowered by about 1 / 4.2, and the electrical conductivity was lowered by 1 / 138.25 when the final peptide powder was prepared. Confirmed losing.

Production example of cosmetics

The red sea ginseng-derived peptides according to the present invention can be prepared in various forms of cosmetics according to the purpose. The following are some examples of the preparation of cosmetics containing red sea ginseng-derived peptides according to the present invention as active ingredients, but the present invention is not limited thereto.

Cosmetic Production Example 1 Preparation of Flexible Lotion

Peptides from Red Sea Ginseng 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium ID 0.05 parts by weight

Allantoin 0.10 parts by weight

Dipotassium glycylizate 0.05 parts by weight

Citric acid 0.01 parts by weight

Sodium citrate 0.02 parts by weight

Glyceres-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated Castor Oil 1.00 parts by weight

ethanol 30.0 parts by weight

Preservative a very small amount

coloring agent a very small amount

Flavor a very small amount

Purified water Remaining amount

Cosmetic Production Example 2 Preparation of Nutritious Cream

Peptides from Red Sea Ginseng 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

glycerin 1.00 parts by weight

D-panthenol 0.10 parts by weight

Plant extracts 3.20 parts by weight

Magnesium Aluminum Silicate 0.30 parts by weight

PEG-40 Stearate 1.20 parts by weight

Stearic acid 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Lipophilic glyceryl stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl Alcohol 3.00 parts by weight

Mineral oil 4.00 parts by weight

Squalane 3.80 parts by weight

Carlylic / Capric Triglycerides 2.80 parts by weight

vegetable oil 1.80 parts by weight

Dimethicone 0.40 parts by weight

Diphosphium glycylizate a very small amount

Allantoin a very small amount

Sodium hyaluronate a very small amount

Tocopheryl Acetate Quantity

Triethanolamine Quantity

Preservative Quantity

Flavor Quantity

Purified water Remaining amount

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the invention is shown in the claims rather than the foregoing description, and all differences within the scope shall be construed as being included in the invention.

Claims (13)

Red sea ginseng is put into fresh water at 15-25 ℃ for 2-4 hours Storage to remove primary salts (step 1);
Removing red sea ginseng from which the primary salt of step 1 was removed and cutting the gut and washing it, and putting it in a fresh water at 5 to 18 ° C. for 10 minutes to 1 hour to remove the secondary salt (step 2);
Fixing red sea ginseng with the second salt removed in step 2 in 95-105 ° C. boiling water added with 0.01% to 0.5% vinegar for 1 to 3 minutes (step 3);
Drying red sea ginseng fixed in the form of step 3 at 40 to 80 ° C. for 12 to 72 hours (step 4);
Grinding the dried red sea ginseng of step 4 into 20 to 100 mesh (step 5);
Mixing purified water with 800 to 1000 parts by weight to 50 parts by weight of the pulverized red sea ginseng powder of step 5 and then ripening at 80 to 140 ° C. for 5 to 30 minutes (step 6);
Inoculate 10 ⁴ CFU / ml to 10 ³ CFU / ml of the microorganism containing protease or peptidase in the red sea ginseng cooked product of step 6 and stirring it at 80 to 250 rpm at 30 to 40 ° C. for 24 to 72 hours. After the first fermentation, killing the bacteria using a autoclave (step 7);
Proteolytic enzyme is mixed in the red sea ginseng fermentation product of step 7 to 0.01 to 1.5 parts by weight relative to 1 part by weight of red sea ginseng powder of step 5, and then 10 to 80 to 90 ° C. after the second fermentation at 50 to 70 ° C. for 3 to 24 hours. Inactivating the enzyme for 30 minutes (step 8); And
Preparing a powder by centrifugation and filtration at 2,500 to 3,500 rpm after the secondary fermentation of step 8, and then concentrating and lyophilizing (step 9);
Method of producing red sea ginseng-derived peptide powder comprising a.
The method of claim 1,
Step 1 is a red sea ginseng in 15 to 21 ℃ fresh water to remove the salt manufacturing method characterized in that.
The method of claim 2,
Step 1 is a red sea ginseng in 17 to 19 ℃ fresh water to remove the salt, characterized in that the manufacturing method.
The method of claim 1,
Step 2 is a manufacturing method characterized in that the red sea ginseng to remove salt by putting in 11 to 17 ℃ fresh water.
The method of claim 4, wherein
Step 2 is a manufacturing method, characterized in that the red sea ginseng to remove salt in 13 to 15 ℃ fresh water.
The method of claim 1,
The vinegar of step 3 is characterized by using vinegar fermented using fruits or cereals.
The method of claim 1,
Step 6 is characterized in that for 10 to 20 minutes to cook at 115 to 125 ℃.
The method of claim 1,
The microorganism comprising the protease (peteidase) or peptidase (peptidase) is a manufacturing method, characterized in that the bacillus subtilis ( bacillus subtilis ).
The method of claim 1,
The proteolytic enzyme of step 8 is characterized in that at least one selected from the group consisting of metallopeptidase and metalloprotease.
The method of claim 9,
The protease is a production method characterized in that the thymolysin (Thermolysin: EC 3.4.24.27. Microorganism-derived protease produced by Bacillus thermoproteolyticus).
Red sea ginseng-derived peptide powder prepared according to any one of claims 1 to 10.
Cosmetic composition comprising a red sea ginseng-derived peptide powder of claim 11.
The method of claim 12,
The composition is an external skin ointment, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion , Moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, nourishing essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser A composition characterized in that it is prepared in one formulation.

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