KR20190039396A - Natural feed additive composition and its preventive and therapeutic effect on Myxozoa - Google Patents

Abstract

The present invention provides a marine feed adding composition for preventing and treating Kudoa which comprises a hot water extract of Rehmanniae Radix, Reynoutria elliptica, Cinnamomum cassia, Terminalia chebula, and Tussilago farfara, an ethanol extract, and an organic solvent extract having any one number of carbons between one to four or a combination of the same as a composition. Therefore, the composition can represent a Kudoa worm killing activation effect for an infection by a Kudoa worm, and can be widely utilized in increasing stability of fishery by increasing a survival rate of fish species and increasing productivity in fishery using the Kudoa worm killing activation effect.

Description

Natural feed additive composition and its preventive and therapeutic effect on Myxozoa for preventing or treating mucus sp.

The present invention relates to a composition for preventing or treating flounder-style parasitic infectious diseases, and more particularly, the present invention relates to a pharmaceutical composition for aquaculture animal including natural extract.

Myxozoa is a mite spore mite. Multivalvulido is a genus Kudoa parasite that is parasitic to seafood mainly and is very broadly infected with various fish species such as flounder, anchovy, sardine, mackerel, salmon and trout. Host specificity. Occurrence areas are also wide in North America, Australia, South Africa, Japan, and the UK, and infect various organs of the fish (gills, muscles, brain,

Recently, Kudoa, a kind of mucus spore mite, is known as a fish parasite that causes economic damage by lowering the product value of fish stocks by causing fish to melt the fish muscle after causing infection.

Kudoa thyrates with four polar capsules were first reported by Yokoyama et al. In Japan in 2004 to cause muscle dissolution in flounder, and recently parasitism of Kudoa septempunctata in the muscles of cultured flounder in Korea has been reported (Matsukane et al., 2010).

Although the exact pathway of human infection to Kudoa is not clear, it is presumed that the infected flounder is infected by feeding the infected flounder with human flounder.

Recently, in Japan, it has been reported that the cause of vomiting and diarrhea within 2 to 20 hours after ingesting the syrup from 2003 is the main symptom, and more than 100 cases of unexplained food poisoning In 2010, 158 cases were generated. 130 out of 158 food poisoning cases occurred in patients who ingested flounder, and the epidemiologic investigation revealed that K. septempunctata was detected in the flounder.

In addition, as a result of food poisoning induction experiment using experimental animals, a food poisoning symptom of diarrhea and vomiting was induced by a Kudo achi, and it was reported as a new food poisoning causative agent. More than 10 ⁶ spores per gram of flounder were identified, and this was defined as a positive criterion (Kawai et al., 2012).

It is also known as a cause of allergy to patients with Allergic Gastrointestinal Syndrome, and the Japanese government is strengthening the test for parasitism of Kudo achung from imported flounder as well as its own flounder.

Currently, the prevention or treatment of Kudo achi infection depends on the repetitive bathing of the external parasiticidal agent, but the treatment efficiency is extremely low due to the little effect of the remedial action, and thus it is necessary to develop more safe and effective drugs.

In Korean Patent No. 10-1096314, not only the effect of reducing the behavioral activity, which is a behavioral sensitization reaction used as an index of drug addiction, but also the c-Fos expression as a neuron activity indicator in the lateral nucleus and striatum of the brain The composition can be used as a pharmaceutical composition for the prevention and treatment of drug addiction and withdrawal symptoms, as well as to prevent drug addiction and withdrawal symptoms containing an extract of guanosyl extract, which can be usefully used as a health functional food, Lt; / RTI > Korean Patent Laid-Open No. 10-2017-0090396 discloses a method for promoting mucus synthesis and secretion, protecting ulcer lesions, promoting tissue regeneration, increasing gastric mucosal blood flow, increasing endogenous prostaglandins, promoting tissue regeneration, inhibiting gastric mucosal damage, inhibiting oxidative stress, Discloses a composition for preventing or treating gastritis and gastric ulcer, which comprises, as an active ingredient, a callus root extract effective for the prevention and treatment of gastritis and gastric ulcer. Korean Patent Laid-Open No. 10-2004-0065376 discloses a natural antimicrobial agent exhibiting excellent antibacterial activity against various bacteria and fungi that cause human skin diseases and exhibiting a wide range of antibacterial spectrum. It is a natural antimicrobial agent which is used in human health products such as daily necessities and cosmetics, Discloses a natural antimicrobial agent containing an extract of anchovy which can be provided as a natural antimicrobial agent raw material. In Korean Patent No. 10-1628407, an electrolytic ozone generator is installed in the vicinity of a farm, electrolytic ozone water is generated by electrolyzing the raw water by the electrolyzed ozone generator, and the generated electrolytic ozone water is stored in an electrolytic ozone water tank And a method of remedying parasitic external parasites in aquaculture is provided, characterized in that a cultured fish is put into the electrolytic ozonated water tank to rescue an external parasite parasitic on aquaculture fish. However, the above-mentioned inventions differ from the present invention, which is effective for prevention and treatment of infection of Kudoa sp. In fish culture using natural extract including hot water and ethanol extract of guinea purpurea, .

The present invention relates to a method for preventing infestation of mollusks, which is a parasite that causes abdominal pain in a consumer who ingests infected fish in a meeting form by easily infecting various fish species including flounder in aquatic culture of fish, And to provide a pharmaceutical composition for the production of aquatic animals using a natural extract which can safely exhibit an infection preventive and therapeutic effect.

In order to solve the above problems, the present invention provides a method for producing a mucilage containing mucilaginous extracts, ethanol extracts, organic solvent extracts of any one of carbon number 1 to 4, A pharmaceutical composition for aquatic animals for the prevention and treatment of spodoptera is provided.

In addition, the aquaculture feed for preventing or treating Kudo infection is composed of a) a plurality of combinations selected from a) 1 kg each of dried bamboo, dried bamboo, dried bamboo, clam, 1 kg and 20 L of purified water are mixed with each other, placed in a hot water extraction apparatus, extracted at 80-100 ° C for 12 hours, and filtered through a filter paper to prepare a hot water extract; B) 1 kg consisting of a plurality of combinations selected from dried persimmon, dried persimmon, persimmon, lettuce, Kwandong, 1 kg each or dried persimmon, persimmon, And the mixture was allowed to stand at room temperature for 7 days to be extracted. The mixture was filtered through a filter paper to obtain a liquid component, followed by concentration and drying to prepare an ethanol extract. And EP diet for aquaculture, and the extract of step (a) or step (b), at a ratio of 1 kg of EP diet to 10 g of EP diet, to prepare a pharmaceutical composition for aquatic animals.

The composition containing the natural medicinal plant extract of the present invention shows the effect of the mucus spp. Killing activity against the mucus spp. Infection, and can be widely utilized for enhancing the fishery stability by improving the survival rate of the species and improving the productivity of the fish.

Infection was detected in seven (4.9%) of four flounder farms in Jeju Island in the study of the mudflies of the mussels of the cultured flounder and wild fishes in Jeju, where the export of domestic flounder was active in Korea. In 2010, it was confirmed that the cultured flounder was infected with Kudo achi, and the number of cases in which cultured flounder was estimated to be the infectious source among the intestinal infectious diseases were as follows.

year 2010 2011 2012 2013 2014 Origin 113 473 407 244 382

Four out of the 11 cases (36.4%) occurred in May, and the higher the temperature, the better the environment is inhabited.

Recently, as the case of infecting Kudo aster, which is a mucus spp., And the case of human infection, interest in the prevention and treatment of parasite infection is increasing. It only relies on the repeated use of external parasitic remedies such as formalin, fresh water and loess.

However, this treatment is also extremely ineffective because it has little effect on remediation, and repetitive baths are likely to affect the cultivation and safety of consumers. Therefore, it is necessary to develop safe and effective drugs and feed additives. There is no development of an infectious agent against mucus spider mite, Kudo aster, and some researchers have been examining the therapeutic effect of amconium hydrochloride (toltrazuril).

Under these circumstances, the inventors of the present invention have made extensive efforts to develop a method for preventing or treating a mudberry spider mite infectious disease using a natural material extract. As a result, it has been found that several herbal medicine extracts are effective for the prevention and treatment of mud- And it was found that there is a synergistic effect on infectious diseases of Kudo aster, which is a parasitic disease, through the combination of them, and the present invention has been completed.

In detail, the natural material of the present invention is Rehmannia glutinosa , Polygonum cuspidatum , cinnamomum cassia , and terminalia chebula), and gwandonghwa (Tussilago farfara ), and their extracts were prepared to confirm the remedial effect against the mucus spp.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

Example 1: Preparation of extracts for natural medicinal materials

Rehmanniae glutinosa is a perennial plant belonging to the genus Scrophulariaceae, and is then stored in the sand and stored in the fresh jujube (生地 黄), its roots are dried in the dry (黄 地 黄, Rehmanniae Radix) (Rehmanniae Radix Preparata) (Jong Hee Park, Jung Kyu Lee (Commercial), Medicinal Plants Book, pp.385-388, 2000). The taste has sweetness, no poison, and has the effect of stopping the thirst by releasing fever, cooling the blood, cooling the blood, etc. (Wang Sun, Kim Bonghyun, Easy Reading Herbalism, Daegu Hanan University Press, pp.42-43 , 2005), and the effect of streptozotocin-induced hyperglycemia on hyperglycemic mice induced by streptozotocin Kim, Jeong-Sang (Rhemanniae Radix), J Korean Soc Food Sci Nutr 33 (7), pp.1133-1138, 2004), liver function improvement (Kim YS et al., Effects of Rehmanniae Radix on the rat Liver Damaged with CCl4, Kor J. Herbology.15 (1), pp.45-47, H et al., Immunosuppressive principles of Rehmanniaglutinosa var. Hueichongensis. Planta Med. 55 (5), pp. 458-462, 1989).

Reynoutria elliptica ) is a perennial herbaceous perennial plant native to Korea. Its leaves are called 虎 杖 葉, and its roots are called 虎 根 根, and it is used for ornamental, mulberry, edible and medicinal purposes. (Acute hepatitis), cold, dryness, osteomyelitis, pain, constipation, women's diseases, swelling, urinary bladder, malaria, cancer (prevention of cancer / cancer / malignant tumor prevention) ), Nocturnal enuresis, gastroenteritis, poisoning, hemorrhoids, bruises, swelling, wind, customs, seawater and jaundice.

The glue is the young branches of Cinnamomum cassia (Camphoraceae) and the branches of camphor. It is used mainly for the sweaty tag, not for cold and menstruation, and for the stomachache.

Gaza is a large-bodied tree, which is native to India, Myanmar, Malaysia, and Sichuan province, China. It also extracts a yellow pigment called myrovalan from juice and is also used as furniture wood. It is said that it is effective for the relieving of the ki and the old fence and the cough, the breathing, the old diarrhea, the dysentery, the sickness, the exfoliation, the digestion and the appetite, It also works.

Kantouhwa has a unique flavor, its taste is spicy and sweet and its quality is warm. In Oriental medicine, it is used in Jinhae, Gadam, Sputum, Pulmonary tuberculosis, and lung abscess. Pharmacological actions have been reported to be beneficial in the treatment of acne, bronchial asthma, and sore throat due to increased secretion of respiratory secretions, respiratory excitement and light anxiety, vasoconstriction, pediatric acute bronchitis.

In the present invention, the dried and dried persimmon, persimmon, persimmon, gargoyle, and persimmon can be easily collected from domestic nature or can be purchased from medicinal herbs. In order to obtain the extract efficiently,

1 Kg of each dried guinea purpurea, guanggeun, gujee, gojae, and kwantong were mixed with purified water (20 L) and put into a hot water extraction apparatus. The resulting mixture was extracted at 80-100 ° C. for 12 hours, filtered through filter paper, , Gamji, Gaza, and Kwandong hwa were prepared.

In addition, 20 L of 95% ethanol was added to 1 Kg of each dried guinea purpur, hoof roe, buckwheat, goat, and kotohwae respectively, and the mixture was allowed to stand at room temperature for 7 days to be filtered. , 8.2 g, 12.6 g, 9.4 g, 11.8 g, and 8.7 g of ethanol extracts of dried persimmon, horseshoe crab, lettuce, lettuce, and gouta were prepared by concentrating and drying.

The solvent of this embodiment is not limited to ethanol and can be extracted as a solvent having 1 to 4 carbon atoms as an organic solvent.

≪ Example 2 > Salcoda activity test for the extract prepared

After extracting each of the extracts of T. japonica, T. japonica, G. japonica, G. japonica, and K. kuwaiticae in DMSO, diluted with PBS and then purified and purified by the extraction method (heat, Ethanol) so as to have respective concentrations.

After inoculation, the cells were incubated at 25 ° C for 6 hours and 24 hours, and the inactivated Kudo spores were counted. The inactivation rate was 20%, 21-40%, 41-60% ++, 61-80% , And 81 to 100% are represented by ++++.

The results of Example 2 for the samples prepared in Example 1 were as shown in Table 2 below.

sample density Incubation time 6 h 12 h Dry
Hot water extract 1000 vg / mL ++ +++ 200 vg / mL + +++ 100 vg / mL - + Dry
Ethanol extract 50 vg / mL +++ +++ 20? G / mL ++ +++ 10? G / mL + ++ Hojo Chang
Hot water extract 1000 vg / mL ++ +++ 200 vg / mL + ++ 100 vg / mL - + Hojo Chang
Ethanol extract 50 vg / mL +++ ++++ 20? G / mL +++ +++ 10? G / mL ++ ++ Anchorage
Hot water extract 1000 vg / mL +++ +++ 200 vg / mL ++ +++ 100 vg / mL ++ +++ Anchorage
Ethanol extract 50 vg / mL +++ +++ 20? G / mL ++ +++ 10? G / mL ++ ++ Go
Hot water extract 1000 vg / mL ++ ++++ 500 vg / mL ++ +++ 100 vg / mL - ++ Go
Ethanol extract 50 vg / mL ++++ ++++ 10? G / mL +++ +++ 1? G / mL + ++ Kandonghwa
Hot water extract 50 vg / mL ++++ ++++ 10? G / mL +++ +++ 1? G / mL + ++ Kandonghwa
Ethanol extract 50 vg / mL ++++ ++++ 5? G / mL + ++++ 1? G / mL + +++ Symbol Display 20% or less: -, 21 to 40%: +, 41 to 60%: ++, 61 to 80%: +++, 81 to 100%: +++

As can be seen from the above results, the extracts of Kwanghwanghae, ethanol extract and Ganja ethanol were most effective for the inactivation of Kudo aquatum during 6 hours treatment, and the extracts of Ganoderma lucidum, , And hot - water extract of Hojangun showed the inactivating effect of mudflies. At 12 hours treatment, hot water and ethanol extracts of dried mung bean, mung bean, mackerel, Gaza, and Kantong showed the inactivating effect of mucilaginous mushroom, which is the most common mushroom.

In addition, the compound extract was tested at the ratio of 10%, 20%, 30%, 30%, 30%, 30%, 30% and 30%, respectively, of the dried mushroom Respectively.

Each of the extracts of the present invention used in the present invention exhibited a remedial effect against the mushroom anchovy, which is an effective mucilaginous insecticidal insecticide, at the effective concentration, and the prevention of aquatic cultured Kudo parasite using these extracts And therapeutic efficacy can be expected. That is, it is possible to achieve the effect of preventing or treating the infection of the Kudoa sp. By preparing feed additives of the aquaculture fish using the hot water or the ethanol extract of Guanxi, Hujangun, Guji, Gaza, Respectively.

& Lt; Example 3 > Preparation of feed and feeding experiment for administration of pharmaceutical composition for fish animal

The composition and general composition of the experimental diets for the administration of the pharmaceutical composition for aquatic animals used in the present invention is such that the protein content is 53 to 56% by weight and the lipid content is 10 to 16% by weight Commercial EP feed was used. Feed size was 9 ~ 11mm, and it was stored at room temperature after purchase. 10% by weight of dried ginger, 30% by weight of dried ginger, 10% by weight of ginger, 30% by weight of Gaza, 20% by weight of Ganoderma lucidum and 20% by weight of Ganoderma lucidum % Of the combined extracts were mixed in a ratio of 1% by weight and then adsorbed on the feed, which was supplied once a day in proportion to the weight of the cultured flounder infected with Kudoa.

In the experimental example of the present invention, a feeding test was carried out on a cultured flounder having an average weight of 500 g infected by Kudoashii over 87% by PCR test. 10% by weight of dried ginger, 30% by weight of dried ginger, 10% by weight of ginger, 30% by weight of Gaza, 1% of Ginger, , And 20% by weight of Kwanghwanghae were adsorbed on commercially purchased EP feed at a ratio of 10 ml, and the contents were fed for 2 weeks. As a result, it was confirmed that the infection rate of Kudoa spp. Was reduced to 50%.

From the results, it was confirmed that the hot water and ethanol extracts of each of the present invention had a ratio of 10% by weight of dried ginger, 30% by weight of ginger root, 10% by weight of ginger, 30% by weight of Gaza sp. Was administered as a feed additive to aquaculture fish as an active ingredient, and thus it was confirmed that the effect of preventing or treating infection of Kudoa can be attained.

The composition comprising the natural medicinal plant extract of the present invention exhibits the kudo anthracnosis killing activity against Kudo achi infection and can be widely utilized for improving the survival rate of fish species and improving the fishery productivity due to improvement of fishery productivity, .

Claims (3)

A pharmaceutical composition for the prevention or treatment of mucus spores infection, which comprises an extract of any one or a combination of two or more selected from the group consisting of hot-water extracts of Guanxin, Guang Geun, Gui Ji,
A pharmaceutical composition for the prevention or treatment of mucus spores infection, which comprises an extract of any one or a combination of ethanol extract of guinea purpura, guangapine, guangdong, and kantong.
A) 20 kg of purified water was mixed with 1 kg of dried red gruel, 1 kg of dried gruel, 1 g of glues and 1 g of dried gruel, dried gruel, dried gruel, After extraction at -100 ° C. for 12 hours, filtration through a filter paper to prepare a hot water extract
B) 20 kg of 95% ethanol was mixed with 1 kg each of a plurality of combinations selected from dried persimmon, dried persimmon, persimmon, and persimmon 1 kg or dried persimmon, persimmon, For 7 days, and then filtered with a filter paper to obtain a liquid component, followed by concentration and drying to prepare an ethanol extract
A method for preventing or treating a Kudo infection in a fish animal characterized in that an EP feed for aquaculture and an extract of step (a) or step (b) are mixed at a ratio of 1 kg: 10 g of an EP diet