KR20040065376A - Anti-bacterial Agent containing extract of Cinnamomi ramulus - Google Patents

Abstract

PURPOSE: Provided is a natural antibacterial preparation which involves excellent antibacterial capacity and wide range antibacterial spectrum with respect to various kinds of bacteria causing skin diseases, and is used as an antibacterial material in skin, dental and hair cleaning products, and residential cleaning and deodorizing sanitary wares. CONSTITUTION: The antibacterial preparation comprises an essential oil extracted from Cinnamomi ramulus. A cosmetic composition, an antibacterial deodorizing composition, or a residential cleaning composition is made with the content of 0.1-10wt.% of the antibacterial preparation.

Description

Anti-bacterial agent containing extract of Cinnamomi ramulus

The present invention relates to the use of the essential oil extract of Keji as an antimicrobial agent, and relates to a natural antimicrobial agent exhibiting a wide range of excellent antimicrobial effect against bacteria, fungi and microorganisms contaminating the human skin and oral cavity. In general, the human skin is a direct contact with the environment, bacterial, fungal infection easily occurs. Staphylococcus sp., Streptococcus sp., And fungi, which are Gram-positive bacteria, are commonly found on the skin. Staphylococcus epidermidis ( Staphylococcus spp . Staphylococcus epidermidis , Staphylococcus aureus and Propionibacterium acnes . These bacteria infect the skin and cause skin diseases such as acne, rashes, ringworm, swelling, and swelling. Conventional chemical fungicides such as Triclocarban and Triclosan have been used for the purpose of sterilizing these bacteria, but they are relatively ineffective against Gram-negative bacteria and fungi. Usage is limited. Fungi that cause skin disease are fungi called Trichophyton mentagrophyte and Epidermophyton floccosum , which cause ringworm on the skin. Regardless of the causative agent, the symptoms infected by these ringworms are called angiosis, which invades and develops on the toes of the ringworm, the ringworm, the ringworm, the ringworm, the ringworm, the foot ringworm, and the nail. In addition, yeast causes seborrheic dermatitis in the scalp, also called seborrheic eczema, the main symptom is the appearance of hair dandruff. The cause of seborrheic dermatitis is unclear, and as the name suggests, sebaceous oversecretion is likely to be the cause, but in practice, abnormal or qualitative sebaceous secretions have not been demonstrated. Only clinically blistering scales appear because sebum accumulates excessively during tearing. The role of Pityrosporum ovale , a type of resident fungus of the skin, plays an important role. The symptom is severe scald of the scalp and is called dandruff. Another disease caused by yeast is Candidiasis, which is caused by Candida albicans on the skin and mucous membranes. In adults, it occurs in areas of high humidity and moisture, such as between fingers, under breasts, and around armor, and in infants and young children, especially in obesity, the skin tends to rub against each other.

In order to treat diseases caused by the above fungi, it is treated with amphoterin B, ketoconazole, itraconazole, zinc pyrithidon, or zincpyrithion. Such chemical fungicides cause hepatotoxicity in long-term use and may cause resistant bacteria and adverse effects on the human body. Oral diseases include Steptococcus mutans , a common bacterium, and fluorine, triclosan, and cetylpyrium chloride are mainly used to suppress the growth of these bacteria. The present invention is an antibacterial activity against a wide range of bacteria and fungi that are essential oils extracted from ginseng for the purpose of preventing the emergence of resistant strains due to excessive use of these antibiotics and chemical synthetic bactericides. The invention was found to be excellent by completing the invention. Conventional techniques for reported cinnamon extracts include skin protection cosmetic compositions comprising Korean herbal extracts (Korean Patent Laid-Open Patent 2002-044266), anti-influenza virus agents (Japanese Laid-Open Patent 1994-199680), and cold treatments (Japanese Laid-Open Patent). 1996-208468), and a bathing agent for preventing hair aging (Japanese Patent Laid-Open No. 1999-116447). As confirmed in the present invention, the essential oil component of Gyeji shows high antibacterial activity against a wide range of bacteria and fungi. So far, Gyeji is used as a herbal medicine, but research on other uses is insignificant.

An object of the present invention is to provide a natural antimicrobial agent having a broad range of excellent antibacterial activity against bacteria and fungi, and containing a safe essential oil component safe for human body.

In addition, the present invention can be used as an antimicrobial component in skin cleansing and cosmetic compositions for the prevention of symptoms and relief of symptoms of bacteria and fungi associated with human skin diseases.

Gage refers to the young branches of the camphor tree Cinnamomum cassia . In Latin, it is called Cinnamomi ramulus. Its shape is long columnar, and there are many branches. The length is 30㎝ ~ 70㎝ and the thick side is 3 ~ 7㎜. The color of the outside is reddish brown with an unusual aroma, sweet taste and a little hot. Known efficacy in oriental medicine to radiate abundant, and let the blood and blood. It is mainly used for sweaty signs, cold and menstruation, and painful stomach.

<Gage extract preparation>

Gyeji is cut in spring and dried in the shade, cut into thin pieces, or cut into small pieces and packed in 500g units and purchased on the market, using essential oils or distillation, a method of producing essential oils. The component was extracted. That is, 500 g fragmented gages into 3 to 5 mm (mm) are put in a 5 liter container that can blow steam, and then steam is injected using a steam generator. At this time, the boiling point is distilled together with the essential oil components and water vapor below 100 ℃, which is condensed through the cooling process through the cooling device and divided into floral water in the lower layer and essential oil in the upper layer to selectively recover only the essential oil components.

<Sterilization effect of cabbage extract against bacteria and fungi>

Minimal inhibition concentration (MIC) measurement was used to evaluate the bactericidal activity of the essential oil extract. Soybean essential oil extract has a poor solubility in water, so it is dissolved in DMSO.DimethylSulfoxide in organic solvent at 10% (w / v), followed by BHIA brain heart infusion agar. And added to the medium of the potato medium (PDA potato dextrose agar) at a predetermined concentration interval to solidify in the petri dish (petridish) to prepare a solid medium, and then take the cultured bacteria, the cells of the fungus one by one platinum After inoculating on the surface of the medium, bacteria were incubated at 37 ° C. for 24 to 48 hours, and fungi were incubated at 30 ° C. for 48 to 72 hours, and then the minimum concentrations were inhibited by observing the growth of the bacteria.

(Unit% W / V) Classification Test strain name 0.1 0.05 0.01 0.005 0.001 Germ Staphylococcus aureus － － + + + Eccoli － － + + + Hudo Monas Eruginosa － + + + + Klapsila pneumoniae － － + + + Propionibacterium Acnes － － － + + Staphylococcus mutans － － + + + Fungus Tricophyton Mentagrophyte － － － － + Epidermophyton Floco Island － － － － + Aspergillus Niger － － + + + Candida albicans － － + + + Phytirosporium obale － － － + +

As shown in the results of the antimicrobial activity test of Table 1, Gyeji essential oil extract has a wide range of antibacterial activity against bacteria, fungi. The bacteria to be tested include Staphylococcus aureus, which infects the pores and induces inflammation, Humonas aeruginosa, which infects the wound, causing green tissue, Klapsila pneumoniae, which causes pneumonia, and sebum in the pores. Acne-producing bacteria, Propionibacterium Acnes, Straptococcus mutans, which causes oral diseases, and fungi include Epicotactone mentagrophyte, which causes athlete's foot, tinea peony, and leprosy. Dermophyton Flocosome, Pytyrosporium Obale, which causes dandruff on the scalp, and Candida Albicans, which causes candidiasis on the skin.

Limonene, lemongrass, and thyme, which are known to have antimicrobial activity among natural essential oil extracts, exhibit antimicrobial activity against mold, but antimicrobial activity against bacteria is 5 ~ 10 times higher than that of MIC. It can be said to have.

<Evaluation of bactericidal effect on the composition containing cinnamon extract>

In the present invention, the body lotion, body cleaner, mouthwash, athlete's foot care products and antibacterial air fresheners for sterilizing fungi floating in the air by using a broad range of bactericidal power of the essential oil extract of Keji It can be provided as an antimicrobial component constituting various sterilizing product compositions. The present invention is described in detail as follows.

A) Systemic cleaners Examples 1 to 3 and Comparative Example 1

The base and detergent extracts were added by weight according to the examples and comparative examples shown in Table 2 to prepare a whole body cleaner composition so as to be uniformly mixed through a process such as stirring, heating, degassing, and cooling in a mixer.

Raw material name Example 1 Example 2 Example 3 Comparative Example 1 One N-lauryl-L-glutamic acid triethanolamine (30% aqueous solution) 20.0 20.0 20.0 20.0 2 N-laurylmethyltaurine sodium (30% aqueous solution) 10.0 10.0 10.0 10.0 3 Sodium lauryl ether sulfate 20.0 20.0 20.0 20.0 4 Polyglyceryl laurate 5.0 5.0 5.0 5.0 5 Lauroyl diethanolamide 5.0 5.0 5.0 5.0 6 Propylene glycol 7.0 7.0 7.0 7.0 7 Keji Essential Oil Extract 0.1 1.0 10.0 - 8 Spices Quantity Quantity Quantity Quantity 9 dyes Quantity Quantity Quantity Quantity 10 Metal ion sealant, antiseptic Quantity Quantity Quantity Quantity 11 Purified water To 100 To 100 To 100 To 100

Experimental Effects of Staphylococcus aureus and Candida Albicans on Skin Cleansing

Experimental results for confirming the bactericidal effect of Staphylococcus aureus and Candida albicans of the essential oil extract of the cultivated essential oil extract when washed with the systemic cleaners prepared in Examples and Comparative Examples are as follows. Five test subjects were selected for each Example and Comparative Example, and then the subjects were marked with an area of width x length and 3x3 cm on the inner side of the lower arm. 0.3 g of the whole body cleaners prepared in each of Examples and Comparative Examples were applied to the indicated areas, and then evenly applied, and after 10 minutes, rinsed with running tap water and dried naturally. Then, incubated in BHI broth, a sterilized liquid medium named after the pre-cultured Staphylococcus aureus and Candida albicans colonies, respectively, and in the area of the left arm marked 3x3 cm inside the underarm of the arm 50ul of Filocus aureus suspension was applied and 50ul of Candida albicans suspension was applied to the area of the right arm and dried. After 6 hours, the number of bacteria was measured in the display area inoculated with the bacteria. After 6 hours, 1 ml of phosphate-buffered solution with 0.1% v / v Triton X-100 was added to each region to fix a plastic ring with a diameter of 2.7 cm. The area in contact with the ring was rubbed evenly for 1 minute, and then the wash solution was extracted to measure the number of bacteria contained in the wash solution. Table 3 shows the average number of bacteria in each test subject after 6 hours for the Staphylococcus aureus inoculated after treatment with the systemic cleaners prepared in the Examples and Comparative Examples. Table 4 shows the values averaged in Examples or Comparative Examples for Candida albicans. As a result, the total number of Staphylococcus aureus and Candida albicans after 6 hours was reduced compared to the number of bacteria inoculated in all examples except Comparative Example 1, which was first applied to the skin and rinsed off. As the amount of Gyeji essential oil extract increases, the bactericidal effect was further increased.

Staphylococcus aureus Example 1 Example 2 Example 3 Comparative Example 1 Number of inoculated bacteria (cfu / ㎠) ^* 5.6 × 10 ⁵ 5.6 × 10 ⁵ 5.6 × 10 ⁵ 5.6 × 10 ⁵ Number of bacteria after 6 hours (cfu / ㎠) 7.2 × 10 ⁴ 1.0 × 10 ³ 1.1 × 10 ¹ 1.1 × 10 ⁶

* cfu / ㎠: unit showing the number of bacteria per unit area, cfu: colony forming unit,

Smaller value means greater sterilization effect.

Candida albicans Example 1 Example 2 Example 3 Comparative Example 1 Inoculated Gunshu (cfu / ㎠) ^* 3.2 × 10 ⁵ 3.2 × 10 ⁵ 3.1 × 10 ⁵ 3.2 × 10 ⁵ Number of bacteria after 6 hours (cfu / ㎠) 1.0 × 10 ⁴ 3.0 × 10 ³ 2.0 × 10 ² 1.1 × 10 ⁵

* cfu / ㎠: unit showing the number of bacteria per unit area, cfu: colony forming unit,

Smaller value means greater sterilization effect.

In addition to showing the bactericidal power in the medium, Gyeji essential oil extract can also be provided as an antimicrobial fragrance composition because it shows the bactericidal power by volatilization.

(B) Antibacterial fragrance Example 1 and Comparative Example 1

Cinnamon extract was naturally volatilized as an essential oil component and could be used as a fragrance to explore antifungal activity by volatilization.

Raw material name Example 1 Comparative Example 1 One Keiji Essential Oil Extract 100 - 2 Rosemary Essential Oil Extract - 100

The test mold is Aspergillus niger, a mold that frequently occurs and contaminates bathroom walls and interior wallpaper when the residential environment is humid. Antimicrobial test method installation of Aspergillus and this Aspergillus or is a filter membrane of this spore is attached to the spores on the acrylic box inside the following 2m ³ size adhered to about 10 ⁴ to the membrane is a filter with a diameter of 5cm and hooking After extracting the essential oil extract at a volume of 0.1 g / day, the Membran filter was suspended in 10 ml of a diluent containing 0.1% of Tween 80 at 12-hour intervals to extract Aspergillus niger spores attached to the Membran filter. dextose agar) plated and then incubated for at least 48 hours in a 30 ℃ incubator. When spores germinate, count the number of germinations to determine the mortality of Aspergillus Niger spores. As shown in [Table 6], even though the rosemary essential oil extract was volatilized, the fungal spores were not killed in the air, but the spores contacted with the volatilized Keji essential oil extract were all killed after 48 hours.

0 hours 12 hours 24 hours 36 hours 48 hours Gyeji Essential Oil Extract 1.2 × 10 ⁴ Spores 9.3 × 10 ³ spores 3.6 × 10 ³ Spores 10 spores 0 spores Rosemary Essential Oil Extract 1.3 × 10 ⁴ Spores 1.2 × 10 ⁴ Spores 1.2 × 10 ⁴ Spores 1.0 × 10 ⁴ spores 1.1 × 10 ⁴ spores

The present invention relates to the use of essential oil (Essential oil) extracted from gage as an antimicrobial agent, showing excellent antimicrobial activity against various bacteria and fungi that cause human skin diseases, and exhibits a broad spectrum of antibacterial spectrum for skin, mouth, hair-related cleaning products and housing It can be used as an antibacterial ingredient in hygiene-related cleaning and antibacterial fragrance-related antibacterial products.

Claims (6)

An antibacterial agent containing an essential oil component extracted from gages. A composition comprising 0.1 to 10% by weight of the antimicrobial agent containing the gage extract of claim 1, based on the total weight of the composition. According to claim 2, wherein the composition is a composition, characterized in that the hygiene for human hygiene. 3. A composition according to claim 2, wherein said composition is a cosmetic composition. The composition of claim 2, wherein the composition is an antibacterial perfume composition for air hygiene. According to claim 2, wherein the composition is a composition, characterized in that the cleaning composition for residential hygiene.