CN103845628B - One is used for the treatment of tetanic medicine of cat and preparation method thereof - Google Patents

Abstract

The invention discloses one and be used for the treatment of tetanic medicine of cat and preparation method thereof, wherein, medicine comprises following raw medicinal material: windproof, crawl earthworm, ching-chieh, dioscoreae septemlobae,rhizoma<b>,</b>Peppermint, cicada slough, fructus ulmi, the root of Dahurain angelica, arisaema cum bile, rhizoma Gastrodiae, rattletop, stiff silkworm, the root of kudzu vine, notopterygium root, scorpio, Ligusticum wallichii, the root of Chinese wild ginger, safflower, the tuber of pinellia, black snake, fern beanstalk, phellandium algae, grub, blue or green bone rattan, Longstamen Onion Bulb, redpunjab sumac root and Hedge Prinsepia Nut. The invention has the beneficial effects as follows: medicine of the present invention, aspect treatment cat lockjaw disease, has the advantage that cure rate is high, curative effect is short and Western medicine does not have, and by diagnosis and treatment, can strengthen cat immunity, reduces the death rate, and cost price is cheap.

Description

A kind of medicine for treating cat tetanus and preparation method thereof

technical field

The invention relates to the technical field of traditional Chinese medicine pharmacy, in particular to a medicine for treating cat tetanus and a preparation method thereof.

Background technique

Feline tetanus is a zoonotic acute infectious disease caused by Clostridium tetani invading wounds, growing and multiplying and secreting Clostridium tetani toxin, characterized by increased central nervous system irritability, muscle and joint stiffness.

Clostridium tetani is a Gram-positive large, periflagellated, non-capsulated, spore-forming bacterium. Clostridium tetani can produce two exotoxins, one is spastic toxin, which mainly acts on the nervous system, and the other is hemolytic toxin.

When the spores of Clostridium tetani enter the cat's wound and deep trauma, the anaerobic environment in the deep part of the wound is conducive to the growth and reproduction of Clostridium tetani, so they proliferate in large numbers and produce exogenous toxins, causing cats to become ill. Under natural circumstances, cats and dogs can be infected, and many other animals and humans can also be infected.

The muscles and joints of the affected cat are spastic and rigid, the cat's gait is stiff, and the tail is raised, showing a typical horn bow posture. The nictitating membrane is paralyzed and protruding, the pupil is enlarged, chewing and swallowing difficulties, and salivation. Enhanced reflectivity to touch, light and sound.

The principle of Western medicine in treating feline tetanus is mainly to ensure the smoothness of the respiratory tract, blood vessels, and urethra. Once a trauma occurs, the foreign body, necrotic tissue, and pus in the wound should be cleaned and removed in time, and rinsed and disinfected with 3% hydrogen peroxide; care should be taken for the sick cat to reduce irritation. The sick cat should be placed in a quieter dark room and fed with attention. Those who cannot eat should be rehydrated; tetanus antitoxin should be injected. Tetanus antiserum is a specific drug for the treatment of this disease. It can be injected intravenously at 100-1000 units/kg body weight for 2-3 consecutive times.

Chinese veterinarians believe that tetanus refers to a disease in which tetanus occurs first, and the evil of wind poison invades through the wound to cause convulsions. The name of tetanus first appeared in the "Taiping Shenghui Fang" in the Song Dynasty, which said: "The body is stiff, the mouth cannot be opened, the limbs are trembling, the bones are painful, and the face is slanted. All these injuries are caused by wind evil, so it is called tetanus. "The evil of wind-toxin exists widely, not only occurs in wounds of the skin and flesh, lax disinfection during surgery, or contamination of the umbilical cord of newborns, or improper handling of childbirth and abortion, or infection of bed sores, can also cause tetanus. Those caused by trauma are called Jinchuang convulsions; those that occur after delivery are called postpartum convulsions;

The cause of the disease is the injury of the skin and flesh, and the evil of wind and poison. The evil of wind-poison invades the inside of the human body through wounds of the skin and flesh. The wind is a yang evil, and good deeds change several times. The external wind triggers the internal movement of the liver-wind, and the wind-poison invades people for a long time, transforming heat and fire, causing the viscera to be out of balance, qi and blood Disharmony, yin damage and yang, and even death due to yin and yang separation. Wind poison on the surface has mild dysphagia, trismus, whole body jerk, mild convulsions, short convulsion period, and long intermission period. Convulsions of limbs due to wind poison, opisthotonus, high fever and chills, muscle spasms all over the body, short intervals, shortness of breath, excessive phlegm and salivation, constipation, difficulty urinating;

Western medicine treats feline tetanus, but the effect is unsatisfactory, the course of treatment is long, the condition develops rapidly, and is difficult to control. Traditional Chinese medicine not only has the advantages of western medicine, but also has a high cure rate in treating cat tetanus. Through syndrome differentiation and treatment, it can enhance cat immunity and reduce mortality. Therefore, Chinese medicine has advantages that western medicine does not have in the treatment of feline tetanus disease, and is also more and more subject to people's favor.

Contents of the invention

The technical problem to be solved by the present invention is to provide a medicine for treating cat tetanus and a preparation method thereof, which has a high cure rate for treating cat tetanus, can enhance cat immunity through syndrome differentiation and treatment, and has low cost and price. Drugs have advantages that western medicine does not have in the treatment of feline tetanus disease.

In order to achieve the above-mentioned purpose of the invention, the present invention provides a medicine for treating feline tetanus, said medicine comprising the following raw materials: Fangfeng, Bognenia, Schizonepetae, Mianzi, mint, cicada slough, turnip, Angelica dahurica , Dannanxing, gastrodia elata, cohosh, silkworm, kudzu root, notopterygium, scorpion, chuanxiong, asarum, safflower, pinellia, black snake, fern, water fennel, grub, green bone vine, scallion, red bran Yang and Ruiren.

Wherein, the parts by weight of each raw medicinal material in the medicine are preferably: 20-30 parts by weight of Radix Fangfeng, 10-20 parts by weight of Dilonga chinensis, 10-25 parts by weight of Schizonepeta fringe, 10-20 parts by weight of Scutellaria chinensis, 10-20 parts by weight of Peppermint. 25 parts by weight, 20-30 parts by weight of cicadas, 15-30 parts by weight of Chinese catkins, 10-30 parts by weight of Angelica dahurica, 15-30 parts by weight of Dannanxing, 10-25 parts by weight of Tianma, 10-30 parts by weight of Cimicifuga, 10-20 parts by weight of silkworm, 15-30 parts by weight of kudzu root, 10-30 parts by weight of notopterygium, 10-25 parts by weight of scorpion, 10-25 parts by weight of Chuanxiong, 10-25 parts by weight of Asarum, 10-25 parts by weight of safflower 20-30 parts by weight of pinellia, 20-30 parts by weight of black snake, 20-30 parts by weight of fern, 10-30 parts by weight of fennel algae, 15-30 parts by weight of grubs, 10-25 parts by weight of vine , 10-30 parts by weight of scallions, 15-30 parts by weight of red bran poplar and 20-30 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can be: 25-30 parts by weight of Fangfeng, 15-20 parts by weight of Dilonga, 15-25 parts by weight of Schizonepeta, 15-20 parts by weight of Mianzizi, 15-25 parts by weight of mint, 22-30 parts by weight of cicadas, 17-30 parts by weight of Chinese catkins, 15-30 parts by weight of Angelica dahurica, 17-30 parts by weight of Dannanxing, 15-25 parts by weight of Gastrodia elata, 15-30 parts by weight of Cimicifuga 15-20 parts by weight of silkworm, 17-30 parts by weight of kudzu root, 15-30 parts by weight of notopterygium, 15-25 parts by weight of scorpion, 15-25 parts by weight of Rhizoma Chuanxiong, 15-25 parts by weight of Asarum, 16 parts by weight of safflower -25 parts by weight, pinellia 25-30 parts by weight, black snake 22-30 parts by weight, fern 25-30 parts by weight, fennel algae 18-30 parts by weight, grub 17-30 parts by weight, vine 15- 25 parts by weight, 15-30 parts by weight of scallion, 17-30 parts by weight of red bran poplar and 25-30 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can also be: 27-30 parts by weight of Fangfeng, 18-20 parts by weight of Dilonga, 18-25 parts by weight of Schizonepeta, 17-20 parts by weight of Mianzizi, 18-25 parts by weight of mint, 27-30 parts by weight of cicada slough, 17-25 parts by weight of Chinese catkins, 17-25 parts by weight of Angelica dahurica, 18-28 parts by weight of Dannanxing, 15-22 parts by weight of Gastrodia elata, 17-25 parts by weight of Cimicifuga 17-20 parts by weight of silkworm, 17-25 parts by weight of kudzu root, 17-25 parts by weight of notopterygium, 18-22 parts by weight of scorpion, 15-20 parts by weight of Rhizoma Chuanxiong, 18-22 parts by weight of Asarum, 18 parts by weight of safflower -22 parts by weight, pinellia 27-30 parts by weight, black snake 22-28 parts by weight, fern 27-30 parts by weight, fennel algae 20-30 parts by weight, grubs 17-28 parts by weight, vine 15- 22 parts by weight, 18-25 parts by weight of scallion, 18-30 parts by weight of red bran poplar and 25-28 parts by weight of rutabaga.

Among them, the parts by weight of each raw medicinal material in the medicine are: 28 parts by weight of Fangfeng, 19 parts by weight of Crawford, 19 parts by weight of Schizonepeta, 18 parts by weight of Scorpio, 20 parts by weight of mint, 28 parts by weight of cicada slough, 28 parts by weight of Chinese turnip 20 parts by weight, 22 parts by weight of Angelica dahurica, 20 parts by weight of Dannanxing, 18 parts by weight of Gastrodia elata, 19 parts by weight of Cimicifuga, 18 parts by weight of silkworm, 19 parts by weight of kudzu root, 20 parts by weight of notopterygium, 20 parts by weight of scorpion, 18 parts by weight of chuanxiong Parts by weight, 20 parts by weight of Asarum, 19 parts by weight of safflower, 28 parts by weight of pinellia, 25 parts by weight of black snake, 28 parts by weight of fern, 25 parts by weight of fennel algae, 18 parts by weight of grub, and 18 parts by weight of vine 19 parts by weight, 19 parts by weight of scallions, 20 parts by weight of red bran poplar and 26 parts by weight of rutabagas.

Wherein, the parts by weight of each raw medicinal material in the medicine can also be: 20 parts by weight of Radix Fangfeng, 18 parts by weight of Phyllostachys chinensis, 12 parts by weight of Schizonepetae, 17 parts by weight of Scutellaria chinensis, 15 parts by weight of Peppermint, and 25 parts by weight of cicada slough 17 parts by weight of catkin, 15 parts by weight of Angelica dahurica, 25 parts by weight of Dannanxing, 19 parts by weight of Gastrodia elata, 17 parts by weight of Cimicifuga, 16 parts by weight of silkworm, 15 parts by weight of kudzu root, 17 parts by weight of notopterygium, 19 parts by weight of scorpion 15 parts by weight of Chuanxiong, 20 parts by weight of Asarum, 20 parts by weight of safflower, 25 parts by weight of Pinellia, 28 parts by weight of black snake, 25 parts by weight of fern, 17 parts by weight of fennel algae, 19 parts by weight of grubs, 22 parts by weight of Guteng, 20 parts by weight of Allium, 17 parts by weight of poplar and 25 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can be again: 22 parts by weight of Fangfeng, 19 parts by weight of Phyllostachys chinensis, 15 parts by weight of Schizonepeta fringe, 19 parts by weight of Scutellaria chinensis, 17 parts by weight of Peppermint, 20 parts by weight of cicada slough 18 parts by weight of catkins, 17 parts by weight of Angelica dahurica, 22 parts by weight of Dannanxing, 20 parts by weight of Gastrodia elata, 22 parts by weight of Cimicifuga, 18 parts by weight of silkworm, 17 parts by weight of kudzu root, 19 parts by weight of notopterygium, 20 parts by weight of scorpion 17 parts by weight of Chuanxiong, 19 parts by weight of Asarum, 18 parts by weight of safflower, 20 parts by weight of pinellia, 25 parts by weight of black snake, 22 parts by weight of fern, 18 parts by weight of fennel algae, 20 parts by weight of grub, 20 parts by weight of Guteng, 22 parts by weight of Scallops, 18 parts by weight of poplar red bran and 20 parts by weight of rutabaga.

In order to solve the above-mentioned technical problems, the present invention provides a preparation method of a medicine for treating cat tetanus. The preparation method comprises the following steps: put all raw medicinal materials into a pulverizer and pulverize them into fine powder, put them into acid and alkali-resistant dipping pot, pour ethanol with an alcohol concentration of 80%-90% relative to the mass of the fine powder into the pot, and soak it for 20-30 days at normal temperature and pressure, and then put the soaked liquid and dregs Squeeze and filter, and concentrate the filtrate under reduced pressure to obtain a paste. Put the dregs obtained by filtration into an oven for drying. The temperature of the oven is 110-115°C, and the drying time is 10-20 minutes. After drying, it is ground into a 150-250 Mesh fine powder, make its fine powder amount reach more than 95% of total material, obtain described medicine.

The beneficial effects brought by the technical solutions provided by the embodiments of the present invention are: the medicament of the present invention has the advantages of high cure rate and short curative effect in the treatment of cat tetanus, and through syndrome differentiation and treatment, cat immunity can be enhanced, mortality rate can be reduced, and the cost price can be reduced. low. Drugs have advantages that western medicine does not have in the treatment of feline tetanus disease.

detailed description

Chinese veterinarians believe that the cause is due to injury of the skin and flesh, and the evil of wind and poison. The evil of wind poison invades the inside by the wounded skin and flesh. The wind is a yang evil, and the good deeds change several times. The external wind triggers the internal movement of the liver wind, and the wind poison invades for a long time, transforming heat and fire, causing the viscera to be out of balance, and the Qi and blood to be out of harmony. , Yin damage and Yang, and even death due to the separation of Yin and Yang. Therefore, the general treatment principle is to suppress wind and relieve convulsions, clear away heat and detoxify; nourish blood and nourish yin, and dredge the meridians.

Therefore, the present invention provides a kind of preparation method of the medicine that is used for the treatment of feline tetanus, and it comprises basic feed ingredient Saccharomyces cerevisiae, lactic acid enterococcus, manganese oxide, ferrous sulfate and medicine; Said medicine comprises following raw materials: , creeping dragon, nepeta ear, cotton scorpion, mint, cicada slough, turnip, angelica, dannanxing, gastrodia elata, silkworm, kudzu root, notopterygium, scorpion, cohosh, chuanxiong, asarum, safflower, pinellia, Black snake, fern, fennel algae, grub, green bone vine, scallion white, red bran poplar, rutabaga.

The pharmacology of each component in the described medicine is as follows:

Fangfeng: pungent in taste, sweet, slightly warm in nature; enters bladder meridian, lung meridian, spleen meridian, liver meridian. "Rihuazi Materia Medica": "Treatment of thirty-six winds, men's all kinds of labor, nourishing the middle and nourishing the spirit, wind red eyes, stopping tears and paralysis, benefiting the five internal organs and channels, five labors and seven injuries, laxity and night sweats, Disturbed weight, can soothe the mind, calm the mind, and even the Qi pulse." "Changsha Yaojie": "Progress the meridians, expel dampness, clear the joints, relieve pain, relax the muscles and veins, stretch the acute twins, activate the limb joints, cause paralysis, and suppress spontaneous sweating , night sweats, broken leaks, and collapse.” Functions expelling wind and relieving the exterior, eliminating dampness and relieving pain, relieving spasm, and relieving itching. For exogenous wind-cold, headache and body pain, rheumatic arthralgia, joint pain, abdominal pain and diarrhea, intestinal wind and blood, tetanus, rubella pruritus, and sores. It should not be used by those who are deficient in yin and blood, or those who suffer from febrile disease and wind.

Schizonepetae: pungent in taste, slightly warm in nature, enters the lung and liver meridian, has the effects of sweating and relieving the surface, purifying toxins, clearing rashes, and blood to stop bleeding. It is mainly used for fever and headache caused by exogenous wind-cold wind-heat, whether sweating or not. Sweat, all can be applied; headache, dizziness, and impermeable rash due to wind-heat attack; postpartum hemorrhage, stroke, intestinal wind and blood, etc. Both Schizonepeta and Fangfeng are pungent, warm and detoxifying products, expelling wind and dispersing cold. Fangfeng is the emollient in wind medicine, which can relieve wind and dampness, raise yang and invigorate temper, but the sweating power is not as good as Schizonepeta; It is also peaceful, with a light temperament, which can dispel wind-cold and wind-heat. What are the uses of the two medicines, and the power of relieving the exterior is enhanced.

Peppermint: pungent in nature and flavor, cool. Go into lung meridian, liver meridian. For anemopyretic cold, the beginning of febrile disease. This product is pungent to disperse, cool to clear away heat, light and cool, and is commonly used to evacuate wind-heat, so it can be used to treat wind-heat cold or early febrile disease, evil in Weifen, headache, fever, slight aversion to wind-cold, often It can be used with silver flower, forsythia, burdock seed, nepeta, etc. For headache and conjunctival congestion, sore throat. This product lightly lifts up and floats, has a fragrant fragrance and clears the orifices, and is good at evacuating wind-heat from the upper burner, clearing the head and throat. It is used to treat wind-heat attack, headache and red eyes, and it is often used together with mulberry leaves, chrysanthemums, and Vitex; it is used to treat wind-heat congestion, sore throat, often with platycodon grandiflorum, raw licorice, silkworm, Nepeta, and Fangfeng. use. Modern research shows that peppermint has antiviral effect; analgesic, antipruritic effect; anti-irritation, cough-relieving effect; bactericidal effect; Freshens breath. Gargle with peppermint tea juice to prevent bad breath. Steaming noodles with mint tea mist also has the effect of shrinking pores. Applying the leaves soaked in tea on the eyes will feel cool and relieve eye fatigue. It is said that peppermint also has another name of "eye grass" and can be used to treat eye diseases. Skin effect: Shrink capillaries, remove toxins from the body, improve eczema, ringworm, relieve itching, inflammation and burns, soften skin, eliminate blackheads, and benefit oily hair and skin. Physiological effects: Peppermint is the best essential oil for treating colds, it can suppress fever and mucous membrane inflammation, and promote perspiration; it has a good effect on clearing throat and eliminating bad breath; the effect of cooling and analgesia can relieve headaches, migraines and Toothache; In addition, it has a good effect on the respiratory system, digestive system, and endocrine system.

Angelica dahurica: expelling wind and cold, clearing the orifices and relieving pain, promoting blood circulation and expulsion of pus, promoting granulation and relieving pain, drying dampness and stopping belt. For breast pain, swelling and pain, headache, toothache, nasal sinusitis, intestinal wind and hemorrhoids, red and white vaginal discharge, carbuncle sores, skin itching. Modern pharmacological studies have proved that Angelica dahurica also has anti-inflammatory, antipyretic and analgesic effects, activating collaterals and dispelling stagnation, and can be used to treat mammary gland agglomeration in combination with other traditional Chinese medicines. External use of Angelica dahurica is an important medicine for beauty. "Compendium of Materia Medica" states that Angelica dahurica "grows skin, moisturizes color, and can be used as facial fat", and it is often used in ancient beauty prescriptions.

Dannanxing: bitter cold, slightly pungent, cool. Return lung, liver, spleen channel. Clear fire and resolve phlegm, calm convulsions and settle chronic diseases. Control apoplectic phlegm obsession, convulsion epilepsy, cough due to phlegm-fire, dizziness due to head wind. "Compendium of Materia Medica": "Treatment of convulsions in children with convulsions and phlegm, convulsions of limbs, qi deficiency and internal heat in adults, heat stagnation and phlegm."

Gastrodia elata: sweet in taste, flat in nature, enters the liver meridian, has the effects of calming the liver and relieving wind, relieving spasm, dredging collaterals and relieving pain, mainly used for dizziness and headache caused by inflammation of the liver, hyperactivity of liver yang, and vigor caused by internal movement of liver wind Wind convulsions, epilepsy and other symptoms.

Cimicifuga: acrid in taste, sweet, slightly cold in nature; returns lung meridian, spleen meridian, large intestine meridian, stomach meridian. "On the Properties of Medicine": "Treating wind in children, convulsions, epilepsy, and heat illness during the season. It can cure phlegm, wind, NI, swelling and pain, floating and rotten roots of teeth, heat, toxin and pus and blood. In addition to heart, lung, wind, toxin, heat, blockage, aphtha, and depression. Carbuncles, pea sores; decocted cotton and wipe the sores." "Compendium of Materia Medica": "eliminate macule rash, promote blood stasis, control yang depression, dizziness, pain in the chest and hypochondriac, heavy diarrhea after prolonged diarrhea, turbidity, belt Down, metrorrhagia, stranguria with blood, bleeding, impotence and foot cold." Function clearing away heat and detoxification, clearing rash. For measles poor penetration, heat-toxin macule, exogenous pathogenic syndrome, seasonal epidemic fire poison, aphtha, sore throat, headache, cold and heat, carbuncle and sore virus, depression of middle-qi, spleen-deficiency diarrhea, chronic dysentery and heavy diarrhea, women take it off , collapsed.

Bombyx mori: salty, pungent, flat. Return liver, lung, stomach warp. Dispels wind and calms convulsions, resolves phlegm and resolves stagnation. (Xifengzhispasm, dispelling wind, relieving pain, resolving phlegm and resolving stagnation) is used for convulsions, convulsions, sore throat, submandibular lymphadenitis, facial paralysis, and itchy skin.

Pueraria: also known as Gange. Sweet and pungent, it has various functions such as relieving exterior and reducing fever, promoting body fluid, promoting rash, promoting yang and relieving diarrhea, etc. It can cure exogenous fever, headache, high blood pressure, neck pain, thirst, thirst, opaque measles, heat rash, and diarrhea and many other diseases. The general pueraria is tough and fibrous.

Notopterygium: pungent in taste, bitter, warm in nature; enters bladder and kidney channels. "On the Nature of Medicine": "Treatment of thieves' wind, aphonia, speechlessness, itching and blood leprosy, paralysis of hands and feet, crooked mouth and face, and stubborn numbness all over the body." The eight winds and thieves can eliminate new and old rheumatism, and expel rotten flesh and sores." "Lei Gong Pao Medicinal Solution": "Qianghuo Qi is clear and belongs to Yang, and it is good at moving Qi, relaxing but not restraining, ascending and sinking, male and good at dispersing, and can be published. Evil, so start with the small intestine of the Taiyang. Foot the bladder of the Taiyang to control the wind, although its function is different from that of the independent living, it is actually exposing and interiorizing each other." The function is to dispel cold from the exterior, dispel rheumatism, benefit joints, and relieve pain. Main exogenous wind-cold, headache without sweating, Feng Shui edema, sore swollen toxin. For impotence and nocturnal emission, enuresis and frequent urination, cold pain in the waist and knees, asthma due to kidney deficiency, diarrhea at dawn; external use for treating vitiligo and alopecia areata. Relieve the exterior and dispel cold, expelling wind and dampness, relieving pain. Notopterygium volatile oil has anti-inflammatory and anti-allergic effects. After intragastric administration, it can inhibit xylene ear edema, carrageenan and dextran foot swelling.

Cicada slough: salty, sweet, cold, non-toxic, "Compendium": "salty sweet, cold, non-toxic." Return to the lung, liver meridian "Materia Medica Zaixin": "enter the liver, spleen, and lung meridians." Indications: Dispersing wind and removing heat, expelling rash, removing nebula. "Compendium": "Cicadas, the main treatment is all wind-heat syndromes. The ancients used the body, and the later generations used the slough. Generally, the body of the viscera is treated, and the body of the cicada is used; the skin sores are treated with wind-heat, and the slough of the cicada is used." For impermeable measles, rubella itching, convulsions and convulsions. Cicada slough has the effect of dispelling wind-heat, and it is often used in conjunction with mint for wind-heat symptoms. It also has the function of dispelling wind and relieving itching for rubella and itching. The cicada sloughs through the hair and has a heat-clearing effect.

Scorpion: taste pungent, neutral in nature, returns to the Liver Meridian, has the effects of calming wind and relieving spasm, attacking toxins and dispelling stagnation, dredging collaterals and relieving pain, mainly treating infantile convulsions, convulsions and convulsions, apoplexy, hemiplegia, tetanus, rheumatism , migraine headache, sores, scrofula and other syndromes.

Chuanxiong: pungent in taste, warm in nature. Return liver, gallbladder, pericardium channel. "Rihuazi Materia Medica" records it: "cures all wind, all qi, all strains, all blood, replenishes five fatigues, strengthens muscles and bones, regulates all channels, breaks persistent blood, nourishes new blood, grows flesh, has a flooded nose, and vomits blood. And blood drowning, hemorrhoids, cerebral carbuncle hair back, scrofula galls, scabies, and drainage of pus and blood stasis "Modern pharmacological experiments on antibacterial effects and other in vitro tests: Chuanxiong on large intestine, dysentery (Song Nei's), deformity, green Pus, typhoid, paratyphoid bacillus and Vibrio cholerae have inhibitory effect.

Herba Asarum: acrid in taste, warm in nature, returns to the lung and kidney meridian, "On the Properties of Medicine": "cure cough and upper qi, aversion to wind, limelight, urgency in hands and feet, soothe the internal organs, add courage, peel off the rheumatism, can stop Eye wind and tears, bright eyes, open chest stagnation, remove toothache, control blood stagnation, women's blood drips and lumbago." "Nihuazi Materia Medica": "Cure cough, eliminate dead muscle sores, and gather in the chest." It has the functions of expelling wind, dispersing cold and relieving pain, warming the lungs and transforming drink to clear the orifices. It is mainly used to treat exogenous wind-cold, headache, toothache, wind-cold and warm arthralgia, exogenous wind-cold, internal stop-cold drink caused by lung-cold cough and asthma, nasal congestion, nasal sinusitis, cloudy eyes and ears. Sore throat, aphthous halitosis and other symptoms, Asarum has obvious inhibitory effect on cellular immunity and humoral immunity.

Safflower: warm in nature, pungent in taste, enters, heart and liver meridian, has the effects of promoting blood circulation and promoting menstruation, dispelling stasis and relieving pain, used for amenorrhea, dysmenorrhea, lochia, lumps in the abdomen, lumps, injuries from falls, sores, swelling and pain, etc. disease, "Materia Medica Classics": red and blue flowers are the key medicine for promoting blood circulation. It mainly refers to postpartum blood dizziness and mouth silence, because the bad blood does not flow down, and it rushes upwards to the heart, so the dizziness, dizziness and mouth silence, enter the heart and liver, and make the bad blood flow downward, and the dizziness and mouth silence will stop on their own. Intra-abdominal colic, due to the incessant bad blood, stillbirth in the abdomen, non-promoting and promoting blood circulation will not conceive; blood stasis will activate blood, so it can stop colic and stillbirth. Red and blue flowers are also blood-flowing medicines, blood halos are relieved, stagnant, and stop immediately. Excessive use can cause blood to flow continuously and cause death. Safflower can slightly excite the heart, reduce coronary artery resistance, increase coronary artery flow and myocardial nutrition. The role of blood flow.

Pinellia: acrid in taste, warm in nature, poisonous, enters the spleen and stomach meridians, has the effects of drying dampness and resolving phlegm, reducing adverse flow and relieving vomiting, dispelling lumps and resolving stagnation, this product is pungent and warm, dry and toxic, mainly enters the spleen and stomach and enters the lungs, It can promote water dampness and reduce adverse qi, and is good at dispelling dampness and phlegm in the spleen and stomach. When water dampness is removed, the spleen will be strengthened and phlegm and salivation will disappear. Anti-vomiting, good medicine for dispelling swelling and dissipating stagnation, used for cough with excessive phlegm, fullness in the chest and abdomen, chest obstruction, chest knot, goiter, scrofula, sore swelling and pain, hysteroid qi, stomach-qi upward reversal, nausea and vomiting, etc. .

Black Snake: sweet, flat, returns Liver Meridian. Function cures mainly: expelling wind, dredging collaterals, relieving spasm. For leprosy and scabies, scrofula malignant sore.

Osmunda fern: taste, bitter, astringent, cool. Indications: heat-clearing and toxic substances removing, reducing swelling and dissipating stagnation. For lung abscess, conjunctivitis; external use for bruises, snake bites, cervical lymph node tuberculosis.

Fennel algae: nature and flavor, pungent; flat. Meridian return: lung meridian. Indications: dispelling wind and clearing heat; relieving cough. Main wind-heat cold; cough; burns. Grub: nature and flavor: 1. "The Classic": "taste is salty, slightly warm." 2. "Bie Lu": "slightly cold, poisonous." Meridian return: "Materia Medica Huiyan": "Enter the Liver Meridian of Foot Jueyin." Indications : Broken blood, promoting blood stasis, dispelling stagnation, clearing milk. Control broken blood stasis, gout, tetanus, sore throat, nebula, erysipelas, carbuncle, hemorrhoids. ① "The Classic": "The main evil blood stasis numbness Qi, broken blood under the side of firm full pain, closed moon, eyes lustful skin, blue nebula albuginea." ② "Bie Lu": "Treatment of vomiting blood in the chest Abdomen does not go away, broken bones and broken blood knots, golden sores are stuffed inside, postpartum cold, and breast milk." ③ "On the Properties of Medicine": "The juice drops in the eyes, removes nebula, governs blood, and relieves pain." ④ "Supplements to Materia Medica" : "main red and white rash, the rash is scratched, and the broken grub gets the juice and applies it." 5. "Rihuazi Materia Medica": "it can be applied to malignant sores."

Catkin: nature and flavor: bitter, warm. ① "The Classic": "Pungent, flat." ② "On the Nature of Medicine": "Bitter and pungent." ③ "Haiyao Materia Medica": "Pungent, warm, non-toxic." Meridians: enter the spleen and stomach . ① "Lei Gong Pao Medicinal Properties Solution": "Enters the lung and spleen meridians." ② "Jade Catalpa Drug Solution": "Enters the Jueyin Liver Meridian." ④ "Materia Medica Zaixin": "Enter the heart and spleen two meridians." Indications: killing parasites, eliminating accumulation. Treatment of abdominal pain caused by worms. Infantile malnutrition, cold dysentery, scabies, malignant sores. ① "The Classic": "Maintain the five internal evils, dissipate the lewdness and warmth in the skin and joints, remove the three worms, and transform food." "On the Properties of Medicine": "It can control the accumulation of cold air, the pain of trusted subordinates, and eliminate the wind and prostitution of the skin joints like insects." ④Meng Shen: "To treat hot sores, smash and apply pig fat. And white honey to treat damp ringworm. Wagyu cheese Treat all sores. Long-term food controls five hemorrhoids." ⑤ "Diet Therapy Materia Medica": "Dissipating gas pain in the abdomen. It can be used to treat ringworm with horse cheese. Kill the poisonous insects in the middle." ⑥ "Haiyao Materia Medica": "Cure cold Dysentery heart qi, killing parasites and relieving pain. Control woman's uterine wind deficiency again, child malnutrition and diarrhea. Xiefei method rheumatism, dry spleen and eliminate cold food, control jaundice, kill insects and remove roundworm." ⑨ "Chinese Medicine Plant Illustrated Book": "Expelling phlegm and diuresis." Qingguteng: nature and flavor, bitter, pungent, cool. It is slightly poisonous. Indications: analgesia, hemostasis, heat clearing, diuresis. For bruises, rheumatic bone pain, stomach and abdomen pain, bronchitis, urinary tract infection; external use to treat traumatic bleeding, sores, furuncles, and swelling.

Allium: Nature and flavor: hard, warm. ① "Wood Classic": "Pungent, warm." ② "Bie Lu": "Bitter, warm, non-toxic." ③ "Qian Jin Shi Zhi": "Bitter, warm, slippery, non-toxic."④ "Yilin Zhuanyao": "Sweet, sour, pungent, warm." Return to the meridian: ① "Decoction Materia Medica": "Start with Yangming Jing." , Hand Shaoyin Heart Sutra." Indications: Regulating qi, broadening the chest, unblocking yang, dispelling stagnation. Control thoracic obstruction, heartache and back pain, abdominal pain and discomfort, retching, diarrhea and dysentery, sores and furuncles. ① "The Classic": "The main gold sores are defeated." ② "Bie Lu": "Return to the bones. Remove cold and heat, remove water vapor, warm the middle and dissipate stagnation. All sores have apoplexy, cold and edema, and apply it." ③ "Qian Jin · "Food Treatment": "It can produce muscles and benefit the puerpera. If the bone can't be swallowed, eat it." ④Tang Materia Medica: "The white one is nourishing and beautiful, and the red one controls golden sores and phoenixes." ⑤ "Diet Therapy Materia Medica": " Control women's red and white leukorrhea." ⑥ "Supplements to Materia Medica": "Tune the middle, the main long-term benefit is not painful, and those who are often evil in the big abdomen, but cook it more." ⑦ "Materia Medica Tujing": "Tonic, detoxification. "" Main beriberi; boil and drink it for the puerpera, easy to give birth." ⑧ "Materia Medica Yanyi": "Pound with honey, apply soup to burn." Stagnant." ⑩ "Compendium": "Treatment of Shaoyin disease Jue Ni diarrhea, dysentery, chest obstruction and tingling pain, lower Qi to disperse blood, anti-abortion." Treat acute lung and asthma." ⑿ "Hen Jing Feng Yuan": "Pounded juice raw drink, can spit out phlegm in the stomach and eat insects." ⒀ "Lingnan Herbal Records": "Pound raw salt and apply sores; iron needles Injury, leaving rust in the muscles, applying it can be sucked out. It can diverge and relieve the surface, invigorate the stomach, and open the diaphragm." ⒁ "Nanjing Folk Herbs": "Small it and apply it externally to treat various boils. In addition, take wild chrysanthemum decoction for oral administration. "

Red Bran Poplar: Indications: ① "Hunan Medicines": "Treating dysentery." ② "Guizhou Medicine Plant Catalogue": "Astringent intestines. Treating dysentery and diarrhea."

Herba Sinensis: Nature and flavor: bitter, flat. Return through: return kidney, stomach meridian. Indications: promoting dampness and removing turbidity, expelling wind and removing obstruction. For gonorrhea cloudy, excessive leucorrhea, damp-heat sores, lumbago and knee arthralgia.

Rhizoma chinensis: nature and flavor: slightly bitter, cool. Indications: inducing diuresis to expel stones, clearing heat and detoxifying, regulating spleen and eliminating stagnation. For urinary calculus, biliary tract calculus, icteric infectious hepatitis, rabies, fungus poisoning, malnutrition in children, swollen gums, carbuncle and furuncle.

Ruiren: nature and flavor: sweet, cold. ① "Bie Lu": "slightly cold, non-toxic." ② "Yi Lin Zhuan Yao": "sweet and salty, cold." Meridian return: enter the liver, heart channel. ① "Lei Gong Pao Medicinal Properties Solution": "Enter the heart, liver, and spleen meridians." ② "Yu Catalpa Medicine Solution": "Start with Taiyin Lung and Foot Jueyin Liver Meridian." Indications: dispelling wind, dissipating heat, nourishing the liver, brightening head. Control conjunctival congestion, swelling and pain, dimness and embarrassment, rotten canthus with tears, epistaxis. ① "Ben Jing": "The main confidant's evil knot qi, eyesight, red eyes, pain, tears." The canthus is rotten, the nose is rotten, the heart is broken, and the phlegm is formed." ④ "On the Nature of Medicine": "Treating epistaxis." : "Specialized in ophthalmology, eliminates swelling and rotten strings of upper and lower cells, removes heat barrier pterygium in left and right canthus, anneals and stops tears, benefits water and generates light."

Wherein, the parts by weight of each raw medicinal material in the medicine are preferably: 20-30 parts by weight of Radix Fangfeng, 10-20 parts by weight of Dilonga chinensis, 10-25 parts by weight of Schizonepeta fringe, 10-20 parts by weight of Scutellaria chinensis, 10-20 parts by weight of Peppermint. 25 parts by weight, 20-30 parts by weight of cicadas, 15-30 parts by weight of Chinese catkins, 10-30 parts by weight of Angelica dahurica, 15-30 parts by weight of Dannanxing, 10-25 parts by weight of Tianma, 10-30 parts by weight of Cimicifuga, 10-20 parts by weight of silkworm, 15-30 parts by weight of kudzu root, 10-30 parts by weight of notopterygium, 10-25 parts by weight of scorpion, 10-25 parts by weight of Chuanxiong, 10-25 parts by weight of Asarum, 10-25 parts by weight of safflower 20-30 parts by weight of pinellia, 20-30 parts by weight of black snake, 20-30 parts by weight of fern, 10-30 parts by weight of fennel algae, 15-30 parts by weight of grubs, 10-25 parts by weight of vine , 10-30 parts by weight of scallions, 15-30 parts by weight of red bran poplar and 20-30 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can be: 25-30 parts by weight of Fangfeng, 15-20 parts by weight of Dilonga, 15-25 parts by weight of Schizonepeta, 15-20 parts by weight of Mianzizi, 15-25 parts by weight of mint, 22-30 parts by weight of cicadas, 17-30 parts by weight of Chinese catkins, 15-30 parts by weight of Angelica dahurica, 17-30 parts by weight of Dannanxing, 15-25 parts by weight of Gastrodia elata, 15-30 parts by weight of Cimicifuga 15-20 parts by weight of silkworm, 17-30 parts by weight of kudzu root, 15-30 parts by weight of notopterygium, 15-25 parts by weight of scorpion, 15-25 parts by weight of Rhizoma Chuanxiong, 15-25 parts by weight of Asarum, 16 parts by weight of safflower -25 parts by weight, pinellia 25-30 parts by weight, black snake 22-30 parts by weight, fern 25-30 parts by weight, fennel algae 18-30 parts by weight, grub 17-30 parts by weight, vine 15- 25 parts by weight, 15-30 parts by weight of scallion, 17-30 parts by weight of red bran poplar and 25-30 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can also be: 27-30 parts by weight of Fangfeng, 18-20 parts by weight of Dilonga, 18-25 parts by weight of Schizonepeta, 17-20 parts by weight of Mianzizi, 18-25 parts by weight of mint, 27-30 parts by weight of cicada slough, 17-25 parts by weight of Chinese catkins, 17-25 parts by weight of Angelica dahurica, 18-28 parts by weight of Dannanxing, 15-22 parts by weight of Gastrodia elata, 17-25 parts by weight of Cimicifuga 17-20 parts by weight of silkworm, 17-25 parts by weight of kudzu root, 17-25 parts by weight of notopterygium, 18-22 parts by weight of scorpion, 15-20 parts by weight of Rhizoma Chuanxiong, 18-22 parts by weight of Asarum, 18 parts by weight of safflower -22 parts by weight, pinellia 27-30 parts by weight, black snake 22-28 parts by weight, fern 27-30 parts by weight, fennel algae 20-30 parts by weight, grubs 17-28 parts by weight, vine 15- 22 parts by weight, 18-25 parts by weight of scallion, 18-30 parts by weight of red bran poplar and 25-28 parts by weight of rutabaga.

Among them, the parts by weight of each raw medicinal material in the medicine are: 28 parts by weight of Fangfeng, 19 parts by weight of Crawford, 19 parts by weight of Schizonepeta, 18 parts by weight of Scorpio, 20 parts by weight of mint, 28 parts by weight of cicada slough, 28 parts by weight of Chinese turnip 20 parts by weight, 22 parts by weight of Angelica dahurica, 20 parts by weight of Dannanxing, 18 parts by weight of Gastrodia elata, 19 parts by weight of Cimicifuga, 18 parts by weight of silkworm, 19 parts by weight of kudzu root, 20 parts by weight of notopterygium, 20 parts by weight of scorpion, 18 parts by weight of chuanxiong Parts by weight, 20 parts by weight of Asarum, 19 parts by weight of safflower, 28 parts by weight of pinellia, 25 parts by weight of black snake, 28 parts by weight of fern, 25 parts by weight of fennel algae, 18 parts by weight of grub, and 18 parts by weight of vine 19 parts by weight, 19 parts by weight of scallions, 20 parts by weight of red bran poplar and 26 parts by weight of rutabagas.

Wherein, the parts by weight of each raw medicinal material in the medicine can also be: 20 parts by weight of Radix Fangfeng, 18 parts by weight of Phyllostachys chinensis, 12 parts by weight of Schizonepetae, 17 parts by weight of Scutellaria chinensis, 15 parts by weight of Peppermint, and 25 parts by weight of cicada slough 17 parts by weight of catkin, 15 parts by weight of Angelica dahurica, 25 parts by weight of Dannanxing, 19 parts by weight of Gastrodia elata, 17 parts by weight of Cimicifuga, 16 parts by weight of silkworm, 15 parts by weight of kudzu root, 17 parts by weight of notopterygium, 19 parts by weight of scorpion 15 parts by weight of Chuanxiong, 20 parts by weight of Asarum, 20 parts by weight of safflower, 25 parts by weight of Pinellia, 28 parts by weight of black snake, 25 parts by weight of fern, 17 parts by weight of fennel algae, 19 parts by weight of grubs, 22 parts by weight of Guteng, 20 parts by weight of Allium, 17 parts by weight of poplar and 25 parts by weight of rutabaga.

Wherein, the parts by weight of each raw medicinal material in the medicine can be again: 22 parts by weight of Fangfeng, 19 parts by weight of Phyllostachys chinensis, 15 parts by weight of Schizonepeta fringe, 19 parts by weight of Scutellaria chinensis, 17 parts by weight of Peppermint, 20 parts by weight of cicada slough 18 parts by weight of catkins, 17 parts by weight of Angelica dahurica, 22 parts by weight of Dannanxing, 20 parts by weight of Gastrodia elata, 22 parts by weight of Cimicifuga, 18 parts by weight of silkworm, 17 parts by weight of kudzu root, 19 parts by weight of notopterygium, 20 parts by weight of scorpion 17 parts by weight of Chuanxiong, 19 parts by weight of Asarum, 18 parts by weight of safflower, 20 parts by weight of pinellia, 25 parts by weight of black snake, 22 parts by weight of fern, 18 parts by weight of fennel algae, 20 parts by weight of grub, 20 parts by weight of Guteng, 22 parts by weight of Scallops, 18 parts by weight of poplar red bran and 20 parts by weight of rutabaga.

In order to solve the above-mentioned technical problems, the present invention provides a preparation method of a medicine for treating cat tetanus. The preparation method comprises the following steps: put all raw medicinal materials into a pulverizer and pulverize them into fine powder, put them into acid and alkali-resistant dipping pot, pour ethanol with an alcohol concentration of 80%-90% relative to the mass of the fine powder into the pot, and soak it for 20-30 days at normal temperature and pressure, and then put the soaked liquid and dregs Squeeze and filter, and concentrate the filtrate under reduced pressure to obtain a paste. Put the dregs obtained by filtration into an oven for drying. The temperature of the oven is 110-115°C, and the drying time is 10-20 minutes. After drying, it is ground into a 150-250 Mesh fine powder, make its fine powder amount reach more than 95% of total material, obtain described medicine.

Example 1 Drug 1

The embodiment of the present invention provides a medicine for treating feline tetanus, which is made of the following raw materials in parts by weight: 28 parts by weight of Fangfeng, 19 parts by weight of Dilonga, 19 parts by weight of Nepeta, and 18 parts by weight Parts by weight, 20 parts by weight of peppermint, 28 parts by weight of cicada slough, 20 parts by weight of Chinese catkins, 22 parts by weight of Angelica dahurica, 20 parts by weight of Dannanxing, 18 parts by weight of Gastrodia elata, 19 parts by weight of cohosh, 18 parts by weight of silkworm, 19 parts by weight of kudzu root 20 parts by weight of notopterygium, 20 parts by weight of scorpion, 18 parts by weight of Chuanxiong, 20 parts by weight of Asarum, 19 parts by weight of safflower, 28 parts by weight of pinellia, 25 parts by weight of black snake, 28 parts by weight of fern, fennel 25 parts by weight of algae, 18 parts by weight of grubs, 18 parts by weight of Ivy vine, 19 parts by weight of Allium alba, 20 parts by weight of poplar red bran, and 26 parts by weight of rutabaga.

The above-mentioned medicine is prepared by the following preparation method: according to the above-mentioned parts by weight, put all the raw medicinal materials into a pulverizer and pulverize them into fine powder, put them into an acid and alkali resistant dipping pot, and set the alcohol concentration of 4 times the mass of the fine powder to 85% Pour ethanol into the pot, soak for 25 days at normal temperature and pressure, then squeeze and filter the soaked liquid and medicinal residues, and concentrate the filtrate under reduced pressure to obtain a paste, and put the filtered medicinal residues into an oven for drying , the drying temperature in an oven is 110°C, the drying time is 15 minutes, and after drying, it is ground into a 200-mesh fine powder so that the amount of the fine powder reaches more than 95% of the total material to obtain the medicine.

Example 2 Drug 2

The embodiment of the present invention provides a medicine for treating cat tetanus, which can also be made from the following raw materials in parts by weight: 20 parts by weight of Fangfeng, 18 parts by weight of Crawford, 12 parts by weight of Schizonepeta, cotton 17 parts by weight of 萆薢, 15 parts by weight of peppermint, 25 parts by weight of cicada slough, 17 parts by weight of Chinese catkins, 15 parts by weight of Angelica dahurica, 25 parts by weight of Dannanxing, 19 parts by weight of Gastrodia elata, 17 parts by weight of cohosh, 16 parts by weight of silkworm, kudzu root 15 parts by weight, notopterygium 17 parts by weight, scorpion 19 parts by weight, chuanxiong 15 parts by weight, asarum 20 parts by weight, safflower 20 parts by weight, pinellia 25 parts by weight, black snake 28 parts by weight, fern 25 parts by weight, 17 parts by weight of fennel algae, 19 parts by weight of grubs, 22 parts by weight of Ivy vine, 20 parts by weight of Allium alba, 17 parts by weight of poplar red bran, and 25 parts by weight of rutabaga.

The above-mentioned medicine is prepared by the following preparation method: according to the above-mentioned parts by weight, put all the raw medicinal materials into a pulverizer and pulverize them into fine powder, put them into an acid-alkali-resistant dipping pot, and set the alcohol concentration 5 times the mass of the fine powder to 90% Pour ethanol into the pot, soak for 20 days at normal temperature and pressure, then squeeze and filter the soaked liquid and medicinal residues, and concentrate the filtrate under reduced pressure to obtain a paste, and put the filtered medicinal residues into an oven for drying , the oven drying temperature is 110 DEG C, the drying time is 10 minutes, and after drying, it is ground into a 150-mesh fine powder, so that the amount of the fine powder reaches more than 95% of the total material to obtain the medicine.

Embodiment 3 Drug 3

The embodiment of the present invention provides a medicine for treating cat tetanus, which can be made from the following raw materials in parts by weight: 22 parts by weight of Radix Fangfeng, 19 parts by weight of Phyllostachys chinensis, 15 parts by weight of Nepeta panicle, cotton 19 parts by weight of 萆薢, 17 parts by weight of mint, 20 parts by weight of cicada slough, 18 parts by weight of Chinese catkins, 17 parts by weight of Angelica dahurica, 22 parts by weight of Dannanxing, 20 parts by weight of Gastrodia elata, 22 parts by weight of cohosh, 18 parts by weight of silkworm, kudzu root 17 parts by weight, notopterygium 19 parts by weight, scorpion 20 parts by weight, chuanxiong 17 parts by weight, asarum 19 parts by weight, safflower 18 parts by weight, Pinellia 20 parts by weight, black snake 25 parts by weight, fern 22 parts by weight, 18 parts by weight of fennel algae, 20 parts by weight of grubs, 20 parts by weight of Ivy vine, 22 parts by weight of Allium alba, 18 parts by weight of poplar red bran, and 20 parts by weight of rutabaga.

The above-mentioned medicine is prepared by the following preparation method: according to the above-mentioned parts by weight, put all the raw medicinal materials into a pulverizer and pulverize them into fine powder, put them into an acid and alkali resistant dipping pot, and set the alcohol concentration of 3 times the mass of the fine powder to 80% Pour ethanol into the pot, and soak for 30 days at normal temperature and pressure, then squeeze and filter the soaked liquid and medicinal residues, and concentrate the filtrate under reduced pressure to obtain a paste, and put the filtered medicinal residues into an oven for drying , the drying temperature in an oven is 115° C., the drying time is 20 minutes, and after drying, it is ground into a 250-mesh fine powder so that the amount of the fine powder reaches more than 95% of the total material to obtain the medicine.

Toxicity test

1. Cumulative toxicity test report

Select 30 healthy mice, 10 females and 10 males, and take the medicine of Example 1 of the present invention as an object. The dosage design is a phase every 7 days, and the doses of each phase are respectively 0.10LD50, 0.20LD50, 0.25LD50, 0.30LD50 , 0.40LD50, the LD50 of the female and male mice of this sample is 4000m part/k part BW (five doses are 500, 750, 1100, 1800, 2400m part/k part BW). The body weight was weighed once every 7 days, the dosage was adjusted, and 0.05ml/10 parts of BW was orally gavaged. After 28 days, no animal death occurred, and the test ended, and after 28 days of increasing doses of the test substance, neither female nor male animals died. With the medicine of embodiment 2, embodiment 3 as object, carry out toxicity test according to above-mentioned method, the result is the same as embodiment 1.

2. Acute toxicity test

40 NIH mice, SPF grade, half male and half male, weighing 15-20 g, were used for acute toxicity test. The mice were divided into two groups at random, 20 in every group, i.e. a control group and an administration group, fasted for 12 hours before the experiment; the medicine prepared in Example 1 of the present invention was dissolved in water, (concentration was 6.75g crude drug/ml , the highest concentration) by gavage, the volume of gavage is 7ml/kg (that is, the single dose is 28.7 crude drug/kg), the control group is given the same amount of normal saline, 3 times a day, and the interval between administration is 8 hours. Continuously observed for 14 days after administration, and recorded the number of toxic reactions and deaths of the mice. The results of the experiment showed that compared with the control group, there was no significant difference in the mice after administration. The experiment continued for 21 days, and the general condition, diet, drinking water, and weight gain of the mice were all normal. Therefore, the acute toxicity of the medicine of Example 1 of the present invention is low, and it is safe for clinical use. Taking the medicines of Example 2 and Example 3 as objects respectively, the toxicity test was carried out according to the above method, and the results were the same as in Example 1.

Clinical Trials

The animal experiment adopts a single-factor comparative experimental design. Take 100 healthy domestic cats at the age of 45 weeks and weighing about 2 kg. Deworming and vaccinations.

During the trial period, they were randomly divided into a test group (the test groups were Example Group 1, Example Group 2 and Example Group 3) and a control group, with 25 animals in each group. The experiment lasted for 60 days, during which they had free access to food and water, and were routinely immunized and dewormed.

Feed as follows:

Control group: basal diet;

test group:

Example group 1: basal diet + 1.5% drug; the above drug is the drug 1 prepared in Example 1 of the present invention;

Example group 2: basal diet + 1.0% drug; the above drug is the drug 2 prepared in Example 2 of the present invention;

Example group 3: basal diet + 1.8% drug; the above drug is drug 3 prepared in Example 3 of the present invention.

The test time is 60 days, prepare to record the amount of feed and remaining material during the test, and calculate the average daily feed intake of each cat per week and the average daily feed intake of the whole period; the test starts and ends at 7:30 in the morning on an empty stomach Weigh and calculate the average daily weight gain of each cat; make daily work records and cat health records. All data were analyzed by variance analysis and Duncan's multiple comparisons using SPSS13.0 software package. Results are expressed as mean ± standard deviation.

For the cats in Example Group 1, Example Group 2, Example Group 3 and the control group, 10 mL of empty venous blood was collected, and 2 mL of heparin anticoagulated blood was used for the number of T lymphocyte subsets (CD3, CD4, CD8), lymphocyte Determination of cell positive rate. The separated serum was used to determine the content of interleukin (IL-2) and γ-interferon. The positive rate of lymphocytes was determined by acid α-naphthyl acetate staining. The contents of IL-2 and γ-interferon were detected by ELISA method, and the kits of IL-2 and γ-interferon were provided by MARKET, an American company. The number of T lymphocyte subsets in peripheral blood was detected by EPICS-XL flow cytometer from Coulter Company, USA.

Table 1 Effects of drugs on tetanus immune function in cats

It can be seen from Table 1 that the levels of IL-2, γ-interferon, positive rate of lymphocytes, CD4, and CD4/CD8 in the serum of the cats in the drug treatment group were all increased compared with those in the control group. It can be seen that the medicine of the present invention improves the ratio of CD4 (T helper cells) and CD4 (T helper cells) to CD8 (T suppressor cells) in cat immune cells, increases serum complement, IL-2, and γ-interferon And other ingredients, to a certain extent, improve the level of non-specific immunity in cats, and enhance the immunity of cats.

Treatment comparison trials:

From May, 2010 to May, 2012, 400 domestic cats were suffering from tetanus, and the 400 cats were randomly divided into 4 groups, respectively Example Group 1, Example Group 2, Example Group 3 and Western medicine treatment group, Use the medicine 1 that embodiment 1 makes, the medicine 2 that embodiment 2 makes, the medicine 3 that embodiment 3 makes respectively to treat and traditional medicine to treat, and wherein, the dosage of embodiment group 1-3 is each Only 0.3g per day, the treatment period is 10-20 days; the traditional medicine in the western medicine treatment group is injection of tetanus antitoxin 10mg/mL and tetanus immune globulin 15mg/mL, once a day, for 15-28 days ; The blank control group did not receive any treatment. Three months were observed after the treatment, and the cure rate and death rate of the cats in each group were recorded. The results are shown in Table 2.

Table 2 Comparison Table of Treatment Effects of Each Experimental Group

As can be seen from the data of Table 2: the therapeutic effect of medicine of the present invention to feline tetanus is obviously better than western medicine treatment group, reduces mortality rate, improves cure rate.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the present invention. within range.

Claims (7)

1. A medicine for the treatment of feline tetanus, characterized in that, the medicine is made of the following raw materials: Radix Fangfeng, Lychee chinensis, Schizonepeta fringe, Scutellaria chinensis, peppermint, cicada slough, turnip, Angelica dahurica, gallbladder Southern Star, Gastrodia elata, Cimicifuga, Bombyx mori, Pueraria root, notopterygium, scorpion, chuanxiong, asarum, safflower, pinellia, black snake, fern, fennel algae, grub, green bone vine, scallion white, red bran poplar and rutabaga ; The parts by weight of each raw medicinal material in the medicine are: 20-30 parts by weight of Fangfeng, 10-20 parts by weight of Dilonga chinensis, 10-25 parts by weight of Schizonepeta, 10-20 parts by weight of Mianzi Parts by weight, 20-30 parts by weight of cicadas, 15-30 parts by weight of Chinese catkins, 10-30 parts by weight of Angelica dahurica, 15-30 parts by weight of Dannanxing, 10-25 parts by weight of Gastrodia elata, 10-30 parts by weight of Cimicifuga, Bombyx mori 10-20 parts by weight, Pueraria 15-30 parts by weight, Notopterygium 10-30 parts by weight, Scorpion 10-25 parts by weight, Rhizoma Chuanxiong 10-25 parts by weight, Asarum 10-25 parts by weight, Safflower 10-25 parts by weight , 20-30 parts by weight of pinellia, 20-30 parts by weight of black snake, 20-30 parts by weight of fern, 10-30 parts by weight of fennel algae, 15-30 parts by weight of grub, 10-25 parts by weight of vine, 10-30 parts by weight of scallions, 15-30 parts by weight of red bran poplar and 20-30 parts by weight of rutabaga. 2. the medicine for the treatment of feline tetanus according to claim 1, is characterized in that, the parts by weight of each raw material medicinal material in the described medicine is: Fangfeng 25-30 weight part, creeping dragon 15-20 weight part , 15-25 parts by weight of Nepeta panicle, 15-20 parts by weight of Scutellaria chinensis, 15-25 parts by weight of peppermint, 22-30 parts by weight of cicada slough, 17-30 parts by weight of Chinese catkins, 15-30 parts by weight of Angelica dahurica, 17 parts by weight of Dannanxing -30 parts by weight, 15-25 parts by weight of Tianma, 15-30 parts by weight of Cimicifuga, 15-20 parts by weight of Bombyx mori, 17-30 parts by weight of Pueraria lobata, 15-30 parts by weight of notopterygia, 15-25 parts by weight of scorpion, 15-25 parts by weight of Rhizoma Chuanxiong, 15-25 parts by weight of Asarum, 16-25 parts by weight of Safflower, 25-30 parts by weight of Pinellia, 22-30 parts by weight of Black Snake, 25-30 parts by weight of Osmanthus fern, fennel algae 18-30 parts by weight, 17-30 parts by weight of grub, 15-25 parts by weight of Ivy vine, 15-30 parts by weight of scallion, 17-30 parts by weight of poplar and 25-30 parts by weight of rutabaga. 3. the medicine for the treatment of feline tetanus according to claim 1, is characterized in that, the parts by weight of each raw medicinal material in the medicine is: Fangfeng 27-30 weight parts, Pontus 18-20 weight parts 18-25 parts by weight of Nepeta panicle, 17-20 parts by weight of Scutellaria chinensis, 18-25 parts by weight of peppermint, 27-30 parts by weight of cicada slough, 17-25 parts by weight of Chinese catkins, 17-25 parts by weight of Angelica dahurica, 18 parts by weight of Dannanxing -28 parts by weight, 15-22 parts by weight of Tianma, 17-25 parts by weight of Cimicifuga, 17-20 parts by weight of Bombyx mori, 17-25 parts by weight of Pueraria lobata, 17-25 parts by weight of Notopterygium, 18-22 parts by weight of Scorpion, 15-20 parts by weight of Rhizoma Chuanxiong, 18-22 parts by weight of Asarum, 18-22 parts by weight of Safflower, 27-30 parts by weight of Pinellia, 22-28 parts by weight of Black Snake, 27-30 parts by weight of Osmanthus fern, fennel algae 20-30 parts by weight, 17-28 parts by weight of grub, 15-22 parts by weight of Ivy vine, 18-25 parts by weight of Scallops, 18-30 parts by weight of poplar and 25-28 parts by weight of rutabaga. 4. the medicine for the treatment of feline tetanus according to claim 1, is characterized in that, the parts by weight of each raw medicinal material in the medicine is: 28 parts by weight of Radix Fangfeng, 19 parts by weight of Craniosaurus, Schizonepetae 19 parts by weight, 18 parts by weight of Scutellaria chinensis, 20 parts by weight of peppermint, 28 parts by weight of cicada slough, 20 parts by weight of Chinese catkins, 22 parts by weight of Angelica dahurica, 20 parts by weight of Dannanxing, 18 parts by weight of Gastrodia elata, 19 parts by weight of Cimicifuga, Bombyx mori 18 parts by weight, 19 parts by weight of kudzu root, 20 parts by weight of notopterygium, 20 parts by weight of scorpion, 18 parts by weight of Chuanxiong, 20 parts by weight of Asarum, 19 parts by weight of safflower, 28 parts by weight of pinellia, 25 parts by weight of black snake, fern 28 parts by weight of 萁, 25 parts by weight of fennel algae, 18 parts by weight of grubs, 18 parts by weight of Chinese vine, 19 parts by weight of Scallops, 20 parts by weight of poplar and 26 parts by weight of rutabaga. 5. the medicine for the treatment of feline tetanus according to claim 1, is characterized in that, the parts by weight of each crude drug in the medicine is: 20 parts by weight of Radix Fangfeng, 18 parts by weight of Proteolonga chinensis, Schizonepeta paniculae 12 parts by weight, 17 parts by weight of Scutellaria chinensis, 15 parts by weight of peppermint, 25 parts by weight of cicada slough, 17 parts by weight of Chinese turnip, 15 parts by weight of Angelica dahurica, 25 parts by weight of Dannanxing, 19 parts by weight of Gastrodia elata, 17 parts by weight of Cimicifuga, Bombyx mori 16 parts by weight, 15 parts by weight of kudzu root, 17 parts by weight of notopterygium, 19 parts by weight of scorpion, 15 parts by weight of Chuanxiong, 20 parts by weight of Asarum, 20 parts by weight of safflower, 25 parts by weight of pinellia, 28 parts by weight of black snake, fern 25 parts by weight of 萁, 17 parts by weight of fennel algae, 19 parts by weight of grubs, 22 parts by weight of Ivy vine, 20 parts by weight of Allium alba, 17 parts by weight of poplar and 25 parts by weight of rutabaga. 6. the medicine for the treatment of feline tetanus according to claim 1, is characterized in that, the parts by weight of each crude medicinal material in the medicine is: 22 parts by weight of Radix Fangfeng, 19 parts by weight of Prognostica chinensis, Schizonepetae 15 parts by weight, 19 parts by weight of Scutellaria chinensis, 17 parts by weight of mint, 20 parts by weight of cicada slough, 18 parts by weight of Chinese catkins, 17 parts by weight of Angelica dahurica, 22 parts by weight of Dannanxing, 20 parts by weight of Gastrodia elata, 22 parts by weight of Cimicifuga, Bombyx mori 18 parts by weight, 17 parts by weight of kudzu root, 19 parts by weight of notopterygium, 20 parts by weight of scorpion, 17 parts by weight of Chuanxiong, 19 parts by weight of Asarum, 18 parts by weight of safflower, 20 parts by weight of pinellia, 25 parts by weight of black snake, fern 22 parts by weight of 萁, 18 parts by weight of fennel algae, 20 parts by weight of grubs, 20 parts by weight of Ivy vine, 22 parts by weight of scallions, 18 parts by weight of red bran poplar and 20 parts by weight of rutabaga. 7. the preparation method of the medicine that is used for the treatment of feline tetanus according to claim 1, described preparation method comprises the following steps: put into pulverizer and be pulverized into fine powder with all crude medicinal materials, put into acid and alkali resistant dipping pot, Pour ethanol with an alcohol concentration of 80%-90% 3-5 times the mass of the fine powder into the pot, soak for 20-30 days at normal temperature and pressure, and then squeeze and filter the soaked liquid and medicinal residues , the filtrate is concentrated under reduced pressure to obtain a paste, and the dregs obtained by filtering are put into an oven for drying. The temperature of the oven is 110-115°C, and the drying time is 10-20 minutes. Powder, make its fine powder amount reach more than 95% of total material, obtain described medicine.