KR20190023916A - Cosmetic compositions for anti-aging or anti-winkle, and method of preparing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging or ameliorating skin wrinkles. More particularly, the present invention provides a cosmetic composition for preventing skin aging or ameliorating skin wrinkles, which comprises extracellular matrix (ECM) containing a purified signal transduction prompt material (STPM) isolated from a bladder of a pig and a fermentation liquid of a tree sap extract as pharmaceutical agents; and a method for producing the same.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for preventing skin aging or improving skin wrinkles and a method for producing the same,

The present invention relates to a cosmetic composition for preventing skin aging or improving skin wrinkles.

Extracts extracted from plants, animals, minerals and the like are widely used as regeneration, prevention and remedy for damaged skin cells and aging cells in order to regenerate cells of skin damaged by ultraviolet rays and physiological aging. However, existing cell regenerants show only a partial response to the regeneration of ultraviolet and aging skin and the effect is very localized.

In addition, an external preparation containing collagen fibers extracted from proteins derived from animal tissues and plant tissues is prepared, and fibroblasts (collagen, proteoglycan, glycosaminoglycan, GAG)) (which is considered to be very likely to occur due to the activation of MMP (matrix metalloproteinase)), thereby regenerating damaged cells.

However, when the collagen fibers of the animal tissue-derived protein and the plant tissue-derived protein are introduced into the subcutaneous tissues, the cell membranes of the tissue cells are closed and the glycoprotein entry is very limited.

In this case, MSM (Methyl Sulfonyl Methane) or IPM (Isopropyl Myristate), which is a sulfur compound, is used as a local absorbent to force collagen into the subcutaneous tissue. However, the collagen of the external influent does not sufficiently exhibit the collagen original function as compared with the collagen derived from the tissue specific cell. The fundamental reason is the lack of intercellular communication media and the resulting mechanisms. That is, it is a result of the absence of signal material for information transmission.

Therefore, the conventional external preparations for skin regeneration of collagen alone have very little effect on the proportion involved in cell regeneration.

Regarding the regeneration of tissue cells, IST (Intracellular Signal Transduction) is performed by STM (Signal Transduction Material), and representative signaling substances are hormones secreted from endocrine cells and acting on target cells through blood. More than a kind of hormones are produced in the endocrine glands, including the pituitary gland, thyroid gland, parathyroid gland, adrenal glands and gonads.

In addition, unlike hormones, neurotransmitters act on neighboring target cells in the secretory signaling stage that affects adjacent cells. Signaling between synaptically linked neurons is transmitted by neurotransmitters. In this case, when a signaling substance occurs in one cell, it acts on a receptor in another cell membrane to transmit the signal.

Therefore, when a mechanism of transmission and reception interaction is established in the tissue signal, various reactions occur such that specific DNA base sequences are rearranged and specific proteins are copied.

The mechanisms that regulate cell renewal, such as mitosis or apoptosis, programmed cell death, and necrosis, all stem from the mechanisms of these signaling molecules. Therefore, since IST is a physiological phenomenon that is involved in all life phenomena, abnormalities in the signal transduction mechanism cause various diseases.

The IST mechanism involves STPM (Signal Transduction Prompt Material). In other words, when the substance that facilitates signal transduction is lacking, the cells constituting the tissue have problems in the ST (signal transduction) process, intercellular mutual information exchange is cut off, and it becomes impossible to control the physiological phenomenon. The disease occurs.

The inventors of the present invention focused on the fact that the bladder of an animal is composed of collagen and is not damaged even when a certain air pressure is applied. Therefore, it has been found that pigs have similar conditions to human body in the study on bladder tissue components of animals .

The present inventors carried out an evolutionary analysis of 9,000 genes common to six mammals including human and pig in porcine genetic information. As a result, mammals whose function to determine tissue and organ shape are similar are mammals, Dogs, and pigs. That is, the inventors of the present invention were able to confirm the possibility of a pig as a model animal for a long-term bio-use.

The inventors of the present invention found that the extracellular matrix (ECM) derived from a pig bladder is in the shape of a tissue, and the physical properties of the tissue, that is, , Compressive strength, elasticity, etc., and it is confirmed that there is a strong tendency to maintain the cell environment while controlling osmotic pressure and ion permeability.

 In addition, the extracellular matrix derived from swine bladder has many growth factors and cytokines and plays a role in determining the function of cells. Especially, it regulates differentiation of cells, increases or decreases cell adhesion, Suggests structural guidance and morphogenesis.

The extracellular matrix derived from porcine bladder is a protein such as collagen and elastin that determines the physical properties of tissue and glycoproteins such as fibronectin and laminin bind to cells and cells It acts as an adhesive to attach the outer substrate, and there are many proteoglycan such as chondroitin sulfate, which tends to maintain the shape and volume of the tissue.

The extracellular matrix derived from porcine bladder contains a large amount of signal transduction promoting material (STPM), which transmits information for smooth cell regeneration between cells and the surrounding environment, so that they actively interact with cells in the tissue , And serves as a support to keep the volume so that it can maintain and perform the function inherent in tissues or organs. For reference, cells are best adapted to an extracellular matrix environment similar to their own, and physiological activity is most active.

Accordingly, it is an object of the present invention to provide a method for the treatment of fibroblast cell glycoproteins (collagen, elastin, hyaluronic acid, chondroitin), which are components of extracellular matrix (ECM) And a method for separation.

Another object of the present invention is to provide a cosmetic composition for preventing skin aging or improving skin wrinkles comprising a fermentation broth of a glycoprotein and a tree sap extract containing a porcine bladder-derived cell regeneration signal transduction promoter.

The cosmetic composition for preventing skin aging or improving skin wrinkles according to the present invention contains an extracellular matrix (ECM) containing a signal transduction promoting material (STPM) extracted from a pig bladder as a medicament.

The extracellular matrix (ECM) may comprise from 5.0 W% to 99 W% of the glycoprotein.

The cosmetic composition for preventing skin aging or improving skin wrinkles according to the present invention further contains 25% to 99% by weight of a sap from the tree sap as a sorbent of glycoprotein in order to absorb the extracellular matrix (ECM) into skin cells can do.

The method for preparing a cosmetic composition for preventing skin aging or improving skin wrinkles according to the present invention is characterized in that swine bladder is washed with saline (NaCl) and 0.65% saline solution, Pulverizing the primary pulverized powder to a size of 300 mesh to 1 nm through freeze-vacuum drying, collecting the secondary pulverized powder, fermenting the pulverized powder in the extracted tree sap, To produce a highly concentrated sap.

The cosmetic composition of the present invention has an effect of preventing skin aging or improving skin wrinkles.

In addition, according to the method for producing a cosmetic composition of the present invention, there is an effect of contributing to development of the functional cosmetic industry.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 shows constituents and contents of a cosmetic composition according to an embodiment of the present invention. Fig.
2 shows the wrinkle change (N = 30) according to the use of an ECM-containing cosmetic product according to an embodiment of the present invention.
Fig. 3 shows an evaluation of wrinkle improvement according to the use of an ECM-containing cosmetic product according to an embodiment of the present invention.
Figure 4 shows the skin density assessment according to the use of an ECM-containing cosmetic product according to an embodiment of the present invention.
5 shows melanin changes (N = 30) due to use of an ECM-containing cosmetic product according to an embodiment of the present invention.
FIG. 6 shows the moisture change (N = 30) according to the use of an ECM-containing cosmetic product according to an embodiment of the present invention.
FIG. 7 shows evaluation of skin adverse reaction according to the use of an ECM-containing cosmetic product according to an embodiment of the present invention.
Figure 8 shows an ECM containing cosmetic product made according to an embodiment of the present invention.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may unnecessarily obscure the subject matter of the present invention. In addition, the terms described below are established in consideration of the functions of the present invention, and these may vary depending on the intention of the manufacturer or custom in the industry, and the definition should be based on the contents throughout the specification.

The present invention relates to a cosmetic composition for preventing skin aging or improving skin wrinkles. More particularly, the present invention relates to an extracellular matrix containing an extracellular signal transduction promoter (STPM) (Collagen, elastin, hyaluronic acid, chondroitin), which is a constituent material of ECM, and a fermentation broth of a tree sap extract, as a pharmaceutical agent, and a method for producing the same.

The cosmetic composition of the present invention is characterized in that an extracellular matrix (ECM) containing a signal transduction promoter (STPM) separated and purified from a pig bladder is contained in a liquid form in an amount of 0.1 W% to 99 W% And the like.

The cosmetic composition of the present invention is prepared by the following process.

Pig's bladder collection - Wash (wash saline so. = 0.9%) - Crushing - Rapid freeze (-35 ° C) - Shredding (300 mesh) - Pressure vacuum air (PVA) - Vacuum drying - Shredding (300 mesh ~ 1 nm) - Powder capture

ECM powder - Agitation with tree sap - Fermentation - Filtering - ECM liquid collection - Extracellular matrix powder -

Extraction of a glycoprotein containing a porcine bladder-derived signaling enhancer (STPM) is accomplished by the following process.

Protein powder extraction, PPE )

Drying process is performed by cold / dry process because there is a risk of tissue destruction due to thermal drying.

(Pig's bladder) containing ice particles at -20 ° C to -35 ° C was subjected to a first shredding treatment with 100 mesh (mesh) to 300 mesh, followed by drying with vacuum pressure (PVA) Shredding (300 mesh ~ 1 nm) of the bladder tissue produces high-purity protein microparticle powder containing the signal transduction promoter (STPM).

The pig bladder may be lyophilized using another method as described above.

For example, the pig bladder is rapidly frozen in liquid nitrogen (liquid at -196 ° C under atmospheric pressure, the critical temperature is -147.21 ° C, the critical pressure is 33.5 atm), frozen pig bladder The protein powder can be extracted by the vacuum drying method.

Fermentation extraction

The protein powder extract is put into a fermentation tank and mixed with a tree sap and a fermentation medium to obtain a glycoprotein ECM liquid containing a signal transduction promoting material (STPM) through a fermentation process.

The fermentation medium is a yeast strain obtained by fermentation for a certain period of time by mixing sap, sugar and enzyme extracted from medicinal trees present in the wild. It has many advantages such as the generation of new organic acids and various decomposition products and active ingredients, the improvement of flavor and the reduction of toxicity through the decomposition action of yeast through fermentation process.

The extract can produce fibroblast collagen, elastin, glycosaminoglycan (GAG), and can produce a signal transduction promoter (STPM) according to a fermentation process.

Tree sap can be used to replace water through the fermentation extraction process.

Tree sap is rich in A. acid for human body and facilitates the production of keratin-binding protein Filaggrin (or Profilaggrin), which stimulates signal transduction promoter (STPM) It plays a big role in helping to build up a signal system between the skin cells and the surrounding environment and to carry out the tissue regeneration process.

Other reasons for the inclusion of tree sap in the extracellular matrix (ECM), including the signal transduction enhancer (STPM) extracted from the pig bladder are signal transduction promoter (STPM), extracellular matrix (ECM) And other functional groups required for cell regeneration to penetrate rapidly into the cells together with the sap.

Tree sap is a liquid that is raised up to the top through the space between the epidermis and the woody part of the tree. It can be collected by passing through the skin after perforating the epidermis and woody part.

Hereinafter, a cosmetic composition for preventing skin aging or improving skin wrinkles comprising an extracellular matrix (ECM) containing a signal transduction promoting material (STPM) isolated from a swine bladder according to the present invention and a fermentation liquid of a tree sap extract Materials to be added will be described.

Tree sap (Tree sap)

The type of tree sap used in the present invention is not limited to the sap collected from the specified tree but can be used by mixing many kinds of sap which are not toxic and have no effect cancellation due to the chemical reaction.

Trees that can extract a large amount of sap by perforating the tree shell and woody part are as follows.

Among the birch trees, the trees that can be used for sap are Betulaceae, Berula ermanii, Betula davurica, Carpinus, Betula schmidtii, etc., You can choose to use sap.

Among the maple trees, the trees that can be used for sap are almond tree, mountain elm, sweet maple tree, and ash tree among the ash trees.

The properties and components of the wood and its sap used in the present invention will be described.

There are about 200 varieties of maple trees. Among them, Sap is a sap from trees that are more than one kind of tree, such as alder tree, mountain elm, maple tree, and mountain tree.

 For example, the physicochemical properties of mixed liquids such as nodule tree, pseudobacia tree, mulberry tree, mountain elk, and birch tree collected from Goesan - gun and Kwangwon - gun, Korea are as follows.

The mixed solution of the maple and the maple is 96.4% moisture, 2.5% solid, 0.07% C.Protein, 0.02% Ash, pH 6.3, specific gravity 1.007. The nucleic acid-related substances in the mixed solution are CMP, UMP, IMP, GMP, AMP, and Hypoxanthine, and the total amount is 0.1 μm / ml. The main free sugars of the maple are sucrose 23 g / L, fructose 0.3 g / L and glucose 0.5 g / L.

The birch is a genus of seven genera and 100 species, including birch, Manchuria, birch, psyllium, cirrus, and aquatic plants.

For example, the physicochemical properties of the birch sap collected from Goesan-gun, Chungcheongbuk-do and Cheolwon-gun, Gangwon-do were analyzed.

Sugar content is from 0.1 brix to 1.8 brix, from 0.1% to 0.3% of C. proteinase, from 50.5 mg / L to 206.0 mg / L of Ca, from 57 mg / L to 181.9 mg / L of K, from 6.6 mg / L of sodium, 0.1 mg / L of Na, 23.2 mg / L of Na, 0.0 mg / L of Fe to 0.1 mg / L of Fe, 2.5 to 42.1 g / L of Glucose, 4.5 to 36.9 g / L of Fructose, g / L to 5.7 g / L.

Aacid is selected from the group consisting of Glutamic acid 0.2-3.2 mg%, Tau 0.3-1.2, Asp 0.2-0.6, Thr 0.1-1.9, Ser 0.1-0.7, Gly 0.1-0.5, Ala 0.1-2.9, Cys 0.1-0.8, (Average) was calculated as 0.7, Met 0.1-0.3, Ile 0.1-0.6, Leu 0.1-0.6, Tyr 0.1-0.2, Phe 0.1-0.6, Lys 0.4-0.6, His 0.3-0.6, Arg 0.1-0.4, ) 2.3-14.2 mg%.

Nucleic acid related substances were CMP 0.2-0.3 ㎛ ㏖ / ml. UMP 0.5-1.2.

The cosmetic composition of the present invention may contain a phenol extract for enhancing the functionality and formulating the composition.

The kinds of Sanjia which can be used for improving the wrinkles of the skin include three kinds of leaves such as three kinds of leaves, rosemary, ginseng, seaweed, bokbunja, bokbunja, mistletoe, nori, pandemic, dandelion, saururus, bellflower, , Ash tree, thistle, hansikcho, odo tree, olivine, rhododendron, olfactory, willow tree, calendula, evening primrose, camellia, press grass,

The trees from which the cell regenerating functional extract can be obtained can be selected from the group consisting of Nettle tree, Noni tree, Spruce tree, Melon tree, Shiitake tree, Boxwood, Indian wood, Chrysanthemum tree, Oak tree, Lily tree, Peony tree, Mulberry tree, Gingko tree, birch, cedar, etc., and more than one kind or more of the roots, stems, leaves, bark and fruits of the above-mentioned trees can be used.

In addition, a liquid extracted from at least one of the stem, bark, leaf, and fruit of one or more kinds of trees selected from the group of horses can be used.

The cosmetic composition of the present invention may further comprise the following additives.

Vegetable Cell regenerator

Vegetable cell regenerants include asparagus extract, soybean extract, ginseng extract, beech tree extract, yeast extract, microbial fermentation metabolites, and ganoderma extract.

Vegetable moisturizer

The vegetable moisturizing agent is selected from the group consisting of avocado extract, althea extract, aloe extract, Mallow extract, oat extract, licorice extract containing gabbden, isocyanuric acid and liquiritin, gossam extract, Extracts of mushroom, extracts of burdock, extracts of cocoon, extracts of cactus, extracts of soap, extracts of chrysanthemum, extracts of japanese japonica, extracts of mulberry bark, extracts of thyme, extracts of thyme, extracts of Cordyceps sinensis, extracts of sorrel, extracts of marigold, extracts of Melissa, extracts of lime, Extracts and the like.

Additional moisturizer

The moisturizing agent may further include glycerin, propylene glycol, 1,3-butylene glycol, pyrrolidonecarboxylic acid or its salt, sodium lactate, lead acid metabolites, white birch, pine shampoo and the like.

fatty acid

Fatty acids include, but are not limited to, lauric, myristic, palmitic, stearic, oleic, linoleic, linoleic, docosahexaenoic, polyunsaturated, 12- And synthetic fatty acids such as acetic acid, isopentanoic acid and the like.

Emulsifier Wax

Examples of the emulsifying wax include mitt, carnauba wax, whale wax, lanolin (liquid acid, reduced, hard), candelilla wax, Montanub wax, emulsifying wax and the like. have.

Esters

The ester may be selected from the group consisting of isopropyl myristate, isopropyl palmitate, butyl stearate, diacyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, Propylene glycol, and the like, and one or more of these may be selected and added.

Vitamins

The vitamins include the following vitamin groups, and one or more of these vitamins can be selected and added.

Vitamin A group: Retinol, Lecinar, Dehydroretinar, Carotene, Lycopene (Pro-Vit.).

Vitamin B group: thiamine, riboflavin, nicotinic acid, pantothenic acid, biotin, corin, anorthositol.

Vitamin C group: Vit C. and derivatives.

Vitamin D group: ergocarciferol, cholecalciferol, and giardactacysterol.

Vitamin E group: Vit.E or derivatives thereof, ubiquinone derivatives.

Vitamin K group: Pete Nazion, Menakin, Menagion, Menagiore.

Other essential fatty acids, carnitine, ferulic acid, Orizanol, Vit.U and others.

Whitening agent

Examples of the whitening agent include paramino, salicylic acid mast, anthranilic acid derivatives, amino acid compounds, benzotriazol derivatives, filimidine derivatives, dioxane derivatives, franc derivatives, nucleic acid derivatives, nicotinic acid derivatives, ester magnesium, and albutine. Can be selected and added.

Promotion of turnover / cell-activating substance

Promoting action of turnover / Cell viability substances include hydroquinone, lactic acid bacterium extract, manure extract, Vit. A, E, Aranthin, Spleen Extract, Thymus Extract, Yeast Extract, Fermented Milk Extract, Plant Extract (Aloe, Golden, Sugar, Burdock, Carrot, Hope, Yorkin, And one or more of these can be selected and added.

Surfactants

Surfactants include surfactants such as anionic surfactants (alkylcarboxylates, alkylsulfonates, alkylsulfates, alkylphosphates), cationic surfactants (alkylamine salts, alkyl quaternary ammonium salts), amphoteric surfactants: Acid type amphoteric surfactants (amino salts, betaine salts), sulfuric ester type amphoteric surfactants, sulfonate type amphoteric surfactants, phosphate ester amphoteric surfactants, nonionic surfactants, natural surfactants, and derivatives of protein hydrolyzate And one or more of these can be selected and added.

Melanin pigment reduction / degradation substance

Examples of the melanin dye reducing / decomposing substance include hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether), and one or more of these can be selected and added.

Astringent agent

The astringent agent may be selected from the group consisting of Pharmacia, Aranthin, Zinc chloride, Caramines, Resorcin, Ferric Chloride and Tannic acid (including Cadene compounds).

pH adjuster

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, and shell calcium.

Hereinafter, embodiments of the present invention will be described in more detail.

Example  1: Manufacture of ECM raw materials

Ten pork bladders were purchased from the slaughterhouse of the Ministry of Agriculture, Forestry and Fisheries, Korea.

Manufacturing Process 1 (MP1): Wash 3 pig's bladder - Rapid freezing - Shredding - Freeze-dried - Filtering - Powder capture

Manufacturing Process 2 (MP2): Wash 2 pig's bladder - Shredding - Alcohol impregnation - Stirring - Filtering - Freeze-dried - Smash - Filtering - Powder capture (Residual alcohol vaporization)

Manufacturing Process 3 (MP3): Wash 3 pig's bladder - Shredding - Fermenter input - Addition of tree sap and microorganisms - Fermentation - Fermentation liquid collection (Containing STPM)

Manufacturing Process 4 (MP4): Wash 2 pig's bladder - Shredding - Fermenter input - Addition of tree sap and microorganisms - Fermentation - Filtering - Freeze-dried - Powder capture (Containing STPM)

In this process, 115 g of ECM powder in MP1, 72 g of ECM powder in MP2, 89 g of ECM, 0.12 g of STPM, 69 g of ECM and 0.11 g of STPM in MP4 were obtained.

After filtering in MP3 or MP4, the ECM containing STPM can be used as a liquid.

pH was measured between pH 5.5 and pH 6.5, and amino acid analysis, SAD-PAGE electrophoresis analysis, gel strength measurement, viscoelastic analysis, denaturation temperature measurement, measurement of change in gel strength according to fermentation time, It was confirmed that ECM was an excellent glycoprotein by measurement of intensity change, measurement of gel formation point and melting point, and viscoelastic analysis.

Example  2: ECM wrinkle improvement Cosmetic formulation Formula

A basic composition of a wrinkle-improving functional cosmetic comprising a mixture of wood sap and ECM containing STPM is prepared in liquid form and its constituent components and content are shown in Fig.

Example  3: Wrinkle improvement clinical test

Table 1 shows the protocol of the clinical test included in the clinical plan of ECM anti-wrinkle cosmetics.

Title of clinical trial Clinical trial (Replica) for improving facial wrinkles and safety evaluation of anti-wrinkle cosmetics containing ECM Step and design Single-organ, double-blind, sham device comparison, split-face comparison study Purpose of clinical trial We would like to check the efficacy and safety of facial wrinkles in cosmetics by applying to adult womens 30 ~ 65 years old (ECM containing cosmetics) with facial wrinkles more than hardness for 3 months every day. Target disease Female 35 to 65 years of age or older with grade 3 Selection Criteria 1) A person who wrote and signed a voluntary agreement to participate in the clinical trial after hearing enough explanation about the matters to be reported to the subject by the trial person
2) Adult women from 30 to 65 years of age
3) Physical examination of dermatologist, grade 3 or higher (grade)
4) Those who have agreed to cease all dermatological treatment or treatment, including improvement of facial wrinkles during the period of the trial
5) Healthy person who does not have grade, chronic physical disease including skin disease Exclusion criteria 1) If you have a systemic or local infection of activity
2) If there is local skin disease that may affect wound healing
3) If there is a skin abnormality such as acne, erythema, microvascular expansion, scarring in test area
4) If you are diagnosed with mental illness
5) If you are a smoker
6) Fractional laser and soft tissue strengthening (eg, filler, fat graft) for skin elasticity enhancement within 6 months before clinical trial
7) If you use external preparations and cosmetics (containing retinolide) to increase skin elasticity within 3 months before clinical test
8) Pregnant, lactating women
9) Those who are judged to be difficult to carry out clinical trials to evaluate other clinicians Period of clinical trial 12 months from the date of approval of the IRB clinical trial plan. However, it may be extended depending on the speed of subject registration. Number of subjects Efficacy evaluation: Test group - 25 subjects, control group - 25 subjects, total - 25 subjects
Jesus including drop-out (25%): 33 in test group, 33 in control group, 33 in total
In the same subject, the face was divided in half and split face was split, so the actual number of subjects was 33 Test Methods This study was designed to evaluate the efficacy and safety of a 12 week (ECM cosmetic) application to wrinkled skin once daily for adult women aged 30 to 65 years with a tress of Grade 3 or greater .
This is a clinical trial to confirm whether the wrinkle improvement effect is confirmed by the wrinkle replica analysis and the visual evaluation of the dermatologist and to approve the safety of the product. The split face was designed as a comparative study.
Applicants should be informed in writing that they will participate in clinical trials on a voluntary basis and randomly assign both cornified wrinkles to the test site and the control site for subjects eligible for selection / exclusion criteria.
Actually (ECM cosmetics) is used for randomly assigned test sites and no treatment is applied to the control sites.
At the 4th, 8th, and 12th weeks of the test, the replica of the skin, the visual evaluation of the dermatologist, the clinical photographing, the subject satisfaction survey, and the abnormal reaction questionnaire / visual evaluation are performed.
The expected adverse reactions are most likely due to the absence of specific adverse reactions when reference is made to the clinical research literature, etc., which is irradiated on the skin. However, as it may cause itching, scurvy, pain, swelling, erythema, pigmentation, etc., which are the adverse reactions of general skin aesthetic treatment, Validity and safety evaluation [Evaluation of effectiveness]
1. Primary efficacy end point
(1) Determination of wrinkles by image analysis of eye tuck wrinkle
(2) Visual evaluation of improvement of wrinkle grade of two dermatologists
2. Secondary Validity Variables
(1) Evaluation of treatment satisfaction of subjects
[Safety evaluation]
(1) Skin reaction: erythema, pain, itching, burning, pigmentation, etc.

The clinical test flow chart is shown in Table 2.

Screening & Treatment Follow-up Visit One 2 3 4 Week O W4 ± 11) W8 ± 11) W12 ± 11) Screening numbering O Written consent O Demographic survey O Examination of history and drug dosage O Subject conformity assessment O Clinical photography O O O O Visual evaluation O O O O Replica production O O O O Evaluation of validity / safety satisfaction questionnaire O O O Device usage history evaluation O O O (number of times) Adverse reaction confirmation O O O O Delivery of test equipment O Recovery of test equipment O

* Visiting 2, Visiting 3, Visiting 4 allow visits within ± 7 days.

Clinical test results of ECM cosmetics containing STPM showed the results of wrinkle change, result of melanin change, result of moisture change, and evaluation of skin adverse reaction according to cosmetic use as follows.

Clinical Trial Results

Test results 1. Wrinkle change (N = 30) due to use of ECM-containing cosmetics is shown in Fig.

FIG. 3 shows clinical trial results - Evaluation of wrinkle improvement, and FIG. 4 shows clinical trial results - Dermal density evaluation.

Test results 2. Melanin changes (N = 30) due to use of ECM-containing cosmetics are shown in FIG.

Test results 3. Water change (N = 30) due to use of ECM-containing cosmetics is shown in FIG.

Test results 4. Evaluation of skin adverse reaction according to use of ECM-containing cosmetics is shown in FIG.

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. This is possible. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined by the equivalents of the claims, as well as the claims.

Claims (4)

Comprising an extracellular matrix (ECM) containing a signal transduction enhancing substance (STPM) extracted from a porcine bladder as an active agent,
A cosmetic composition for preventing skin aging or improving skin wrinkles.
The method according to claim 1,
The extracellular matrix (ECM)
Characterized in that it comprises from 5.0 W to 99 W% of the glycoprotein.
A cosmetic composition for preventing skin aging or improving skin wrinkles.
3. The method according to claim 1 or 2,
Characterized in that it further comprises 25% to 99% by weight of the extract of the tree sap as an absorbent of the glycoprotein in order to absorb the extracellular matrix (ECM) into skin cells.
A cosmetic composition for preventing skin aging or improving skin wrinkles.
Washing the swine bladder with a chelating agent and a 0.65% saline solution (NaCl), and firstly pulverizing the swine bladder from 100 mesh to 1,000 mesh through rapid freezing and freezing;
A step of secondarily pulverizing the primary pulverized powder to a size of 300 mesh to 1 nm through freeze-vacuum drying, and collecting the second pulverized powder; And
Fermenting the collected powder in the extracted tree sap, filtering, and preparing a highly concentrated sap solution
Wherein the cosmetic composition is a composition for preventing skin aging or improving skin wrinkles.

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