KR20180100854A - Production method of amino acids powder using hydrolysis of Tenebrio molitor and the amino acids powder by this method - Google Patents

Abstract

The present invention relates to a method for producing amino acid powder through hydrolysis of Tenebrio molitor, and amino acid powder produced thereby. More specifically, the method comprises: 1) sterilizing Tenebrio molitor for medical and food uses, selected from the group consisting of larva, pre-pupa, and pupa, and preprocessing the same via freeze-drying if needed; 2) adding ethanol to the Tenebrio molitor to extract proteins therefrom; 3) treating the extracted proteins with proteolytic enzymes to hydrolyze the proteins into amino acids; and 4) filtering the amino acids obtained through hydrolysis, and concentrating the amino acids into solids. The Tenebrio molitor amino acid powder produced according to the present invention is amino acid powder of good quality with an increased content of essential amino acids, and thus is suitable for medical and food uses.

Description

[0001] The present invention relates to a method for producing amino acid powder by hydrolysis of brown gruel and an amino acid powder prepared by the above method,

The present invention relates to a process for producing amino acid powders by hydrolysis of brown gorges and to amino acid powders prepared by the method. More particularly, the present invention relates to a process for preparing amino acids by hydrolyzing amino acids, The present invention relates to a method for producing an amino acid powder of brown gruel which is concentrated to a solid content and an amino acid powder prepared by the above method.

The brown goose (Tenebrio molitor) belongs to the beetle family. It is native to South America. It has a history of about 100 years of breeding in China. It began to be imported from the Soviet Union in the Beijing Zoo in the 1950s. .

In China, it is recognized as yellow insect, which is called yellow mealworm in English and can be used for edible, feed and industrial purposes, unlike other kinds of gourd commonly known as stored grain insects (eg, lesser mealworm) have. Studies on brown ducks have been carried out mainly on physiological studies related to immune function in Europe, and studies on the effects of adult life on hatching rate, sex pheromone on brown duck, study on oxygen concentration and survival rate, . In recent years, studies for industrialization have been carried out, and there have been studies such as mating behavior behavior of the first dwarf, the effect of immune function and body color on the life span and the number of eggs, and many studies on mass production in China are under way. It is known that natural brown ducks grow first in the year, but it is possible to develop multi-generations during the year depending on the temperature of rearing. It grows largely after it grows to the pupa of the next year. The adult life span is between 50 and 160 days, commonly known as 60 to 90 days, the spawning period is between 22 and 137 days, and the average egg scattering is between 400 and 500, with an egg duration of about 1 to 2 weeks. It is milky white with an egg length of 1.25㎜ and has long oval shape and milky white color. Egg shell is weak and weak, and secretes mucus when it is spawning and attaches food or excretion to protect it.

The larvae are completely transformed and change from milky white to yellowish brown when ripened. The larval period is about 4 ~ 10 months and it is known to peel out 14 ~ 15 times. The littered pupa is about 15-19 mm in size and is tawny buff. It can distinguish between male and female with the nipple (nipple) at the end of the abdomen. After 1 ~ 2 weeks, it is alienated. After 4 ~ 5 days after mating, mating and spawning are started. Brown ducks are mating several times throughout their lives, and the amount of spawning is known to be 5 to 15 (up to 30).

The body length of the brown goose is about 15mm, and the color of the body is dark brown and glossy like Kowloon Chowder. There are dots on the head but not densely. The tip of tactile sense is the same length and butterfly. The back of the breast is square, the new groove of the rear rim is distinct, and the middle is a pentagram with a wide bow. The wing wings are brown, with eight new grooves, the convex side of the liver, and small dots. Adult larvae lay eggs mainly in grains, eggs are so small that they can not be easily found by the eyes and hatch after 12 weeks. Extracts of brown goats are called mealworm, and they are largely reared in food insects and pets. The duration of the extract is about 10 weeks, but about two weeks when artificially raising. The reason that wheat worms are used for pets is that they are relatively clean insects because they have relatively short periods of transformation, and they are very clean insects that feed on grain. The extract is molted 920 times depending on the prey and temperature until it becomes a pupa. It becomes a pupa and becomes an adult after 23 weeks. When it first becomes allegory, it changes to light brown or gradually black. Adults are nocturnal, hiding in the hole during the daytime and active at night. It grows in pupa and adultery in spring when it is wintering with extract in the grain bag near the main shrine. It is famous for grain pests and has been growing in recent zoos as food for small animals such as birds and hedgehogs, and is distributed throughout Korea and around the world.

Currently, the patent using brown gurus includes a brown gurr-containing feed (Patent Publication No. 10-2011-0054692), an antifungal protein isolated from a brown gurr extract (Korean Patent No. 10-0230959) .

Accordingly, the inventors of the present invention have completed the present invention by confirming that it is possible to produce a high-solids amino acid powder by hydrolysis using a protein hydrolyzing enzyme while obtaining a method for obtaining an amino acid from a brown gourd having a high essential amino acid content .

It is an object of the present invention to provide a process for producing an amino acid powder having a high content of essential amino acids and solids with good quality by hydrolysis of a brown gruel with a protein hydrolyzing enzyme and an amino acid powder prepared by the above method.

In order to accomplish the above object, the present invention provides a method for preparing a medicament for the purpose of: 1) eliminating intestinal odor by fasting a brown duck selected from the group consisting of a larva, a pre-pupa and a pupa, A pretreatment step of sterilizing for use and lyophilizing if necessary; 2) extracting the protein by adding alcohol to the brown gourd; 3) hydrolyzing the extracted protein by treatment with a proteolytic enzyme to an amino acid; And 4) filtering the amino acid obtained by the hydrolysis and concentrating the amino acid by solidification.

In addition, the present invention provides a brownish rough amino acid powder prepared by the above method.

Hereinafter, the present invention will be described in detail.

The present invention relates to: 1) a method for removing intestinal odor by fasting a brown duck selected from the group consisting of a larva, a pre-pupa, and a pupa, sterilizing it for use in a food medical application, A pretreatment step of freeze-drying according to 2) extracting the protein by adding alcohol to the brown gourd; 3) hydrolyzing the extracted protein by treatment with a proteolytic enzyme to an amino acid; And 4) filtering the amino acid obtained by the hydrolysis and concentrating the amino acid by solidification.

Preferably, the pretreatment step of step 1) further comprises a step of removing the intestinal odorant by fasting the brown gorilla. In the method of the first step, It is preferable that the sterilization is performed at 110 ° C for 5 minutes at a pressure of 1.0 kgf / cm ^{2 and at} a pressure of 1.3 kgf / cm ² at 120 ° C for 10 minutes. The lyophilization in the above step 1) It is preferable to dry the mixture for 48 hours to remove moisture and pulverize it into powder.

In addition, in the method for preparing the brownish rough amino acid powder of the present invention, the protein extraction in the step 2) is preferably performed by adding 10 times the weight of the brown gruel of 30% alcohol, and extracting twice at 90 ° C for 3 hours , Wherein the extraction comprises removing the residues with a Wattman filter (No. 2) or centrifugation (5,000 rpm, 30 minutes) and recovering the extract.

In addition, in the method for producing the brownish-tinged amino acid powder of the present invention, Protamex 0.5% (w / w) is preferably used as the protease of step 3) and hydrolysis is preferably performed for 4 hours.

In addition, in the method for producing the brownish-tinged amino acid powder of the present invention, the concentration to the solid content in the step 4) may be carried out at a temperature of 70 ° C or lower under reduced pressure rotary evaporation using a rotary vacuum evaporator to a concentration of 15-50% Solid content.

In addition, in the method for producing the brownish-tinged amino acid powder of the present invention, it is preferable to further include a step of filtration, spray drying and granule seed preparation after the step 4) (200 占 폚), lyophilized, and then sieved to 50 mesh by adding 1,000 ml (10 times, v / w) of distilled water to the solid. mL, and the mixture is granulated with an oscillator to prepare fine granules having a particle size of 0.1-0.5 mm by hot air drying at 50 ° C or lower for 4 hours.

In addition, in the method for producing the brownish rough amino acid powder of the present invention, after the step 4), it is preferable to further include a step of producing a filtrate and a concentrate for spraying, wherein the concentrate for spraying comprises 15 to 40% By mixing and stirring the volatile concentrate extracted from the brown shoemaker with an excipient.

In addition, in the method for producing the brownish rough amino acid powder of the present invention, it is more preferable to include a step of further drying in a moisture content of 5% or less and bulk packing using an aluminum pouch or a fiber drum.

In addition, the present invention provides a brownish rough amino acid powder prepared by the above method.

The present invention having the above-described structure is suitable for use in food and medicine by providing a high-quality amino acid powder having an increased quality of essential amino acids.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings. However, it should be understood that the following embodiments are provided so that those skilled in the art may understand the present invention without departing from the scope and spirit of the present invention. It is not.

≪ Example 1 > Preparation of brownish rough amino acid powder 1

The present inventors breed brown goose larvae by allowing the brown egg-laying adults to lay eggs at about 26 ° C and a relative humidity of 45% for about 10 days. Bran and cabbage leaves were fed for feeding while brown goat larvae were raised.

The brown duck larvae raised in this way were fasted for three days before collecting and removing the foreign body odor. The larvae were sterilized at 110 ° C. and 1.0 kgf / cm ² at 120 ° C. and 1.3 kgf / cm ² for 5 and 10 minutes, respectively, using a sterilizer, and then subjected to moisture in a freeze dryer (PVTFD30R) Dried and crushed until removed.

The extract was subjected to filtration through a Wattman filter (No. 2) or centrifugation at 5,000 rpm for 30 minutes to remove residues. The extract was washed twice with 30% ethanol for 3 hours at 90 ° C .

The extract was hydrolyzed with 0.5% (w / w) Protamex protease for 4 hours, and then filtered with a Wattman filter (No. 2).

The extract was concentrated under reduced pressure using a rotary vacuum evaporator at a temperature of 70 ° C or less to a solid content of 15 to 50%. The solid content was measured with a refractometer and then 20 mL was recovered and measured by freeze drying.

The extract was filtered again with a Wattman filter (No. 2), spray-dried (200 ° C) with 1,000 mL (10 times, v / w) of distilled water, and lyophilized Respectively. 50 g of 85.5% alcohol was added to 250 g of the powder, mixed well, and microgranules were prepared with an oscillator. Then, the granules were hot-air dried at 50 ^° C or less for 4 hours, To prepare granular seed particles having a particle size of 0.1-0.5 mm. The prepared particles were dried to adjust the moisture content within the fluidized bed granulator to within 5%. Finally, bulk packing was carried out using an aluminum pouch, and a fiber drum was used if necessary.

≪ Example 2 > Preparation of brown gummy amino acid powder 2

The same as Example 1, except that the volatile concentrate and the excipient extracted from the same raw material were mixed in a concentrate having a solid content of 15 to 40% instead of the granular seed particles according to the test ratio and stirred to prepare a spray concentrate, . The rest of the manufacturing process is the same.

<Experimental Example 1> Determination of quality characteristics according to an enzyme treatment method

After Protamex protease treatment in Example 1, the pH was measured using a pH meter and the Brix was measured using a refractometer. As a result, a pH of 7.0 and a Brix value of 13 to 15 were confirmed.

The solids content was determined by evaporating the dried sample at 2OmL at 105 ℃ and measuring the weight (%) of the sample solution. As a result, 35.4% of dry matter was confirmed.

를 사용하여 계산된다.Crude protein content was determined by semimicro-Kjedahl method. Semimicro-Kjedahl (mg / g) method

&Lt; / RTI &gt;

At this time, S = sample weight (g)

T = shape of beef in 0.1 N HCl (mL)

As a result, the crude protein content of the brown gruel powder of Example 1 was found to have a protein content of 96.5% or more.

<Experimental Example 2> Confirmation of quality characteristics of enzyme hydrolyzate

Amino acid composition of the hydrolyzate treated with enzyme was confirmed by amino acid analyzer. As a result, all of the essential amino acids were contained in the brownish brown amino acid powder of the present invention, and it was confirmed that the yield of amino acid production was very high.

<Experimental Example 3> Effectiveness of enzyme hydrolyzate-related antioxidation

&Lt; 3-1 > ACE inhibitory activity

The ACE (angioensin I converting enzyme) inhibitory activity of edible insect hydrolysates was determined by reacting 5 mM Hippuryl-L-His-L-Leu, which is a substrate, with ACE enzyme solution and reacting the supernatant of hydrolyzate at 37 ° C. for 30 minutes . The reaction was stopped by adding 1 N HCl, and the mixture was stirred for 15 minutes with ethyl acetate. The supernatant was then dried at 120 ° C for 30 minutes, and dissolved in distilled water. The absorbance was measured at 228 nm to measure ACE inhibitory activity .

As a result, the ACE inhibition rate of the brown gruel powder prepared in Example 1 was 56.7%, which was much higher than the ACE inhibition rate (9.91%) of the general protein hydrolyzate.

Hydrolysis of brown goats using Protamex was performed at 1 hour intervals from 0 to 8 hours. The samples were heated in a water bath at 100 ° C for 15 minutes to stop the hydrolysis, and the ACE inhibition effect was measured .

When hydrolysis was carried out, ACE inhibition effect was high, and ACE inhibition effect according to hydrolysis time was confirmed. After addition of Protamex, the ACE inhibitory effect was increased rapidly. The ACE inhibitory effect was the highest at 55.5% when the hydrolysis time was 5 hours, and it was confirmed to be maintained thereafter

<3-2> Measurement of electro-donating ability (EDA)

The hydrolyzate was mixed well with 1,1-diphenyl-2-picrylhydrazy (DPPH) solution, left at room temperature for 30 minutes and measured at 520 nm.

The electron donating ability of the hydrolyzate was determined as the percentage difference between the absorbance of the sample and the non-adhered sample, and the IC ₅₀ (inhibition concentration) was used to reduce the EDA (%) of the hydrolyzate by 50%.

As a result, it was confirmed that the IC ₅₀ of DPPH radical of the brownish rough amino acid powder of the present invention was 2.63 ± 0.74, which is higher than that of ascorbic acid of 8.39 ± 0.50.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, This is possible.

Claims (15)

1) Pre-treatment step in which the intestinal odor is removed by pausing the larvae, a pre-pupa and a pupa, and sterilized;
2) extracting the protein by adding alcohol to the brown gourd;
3) hydrolyzing the extracted protein by treatment with a proteolytic enzyme to an amino acid; And
4) The amino acid obtained by the hydrolysis is filtered and concentrated to a solid content.
The method according to claim 1, further comprising a step of sterilizing the brown gill in step 1), followed by lyophilization and pulverization.
The method according to claim 1, wherein the sterilization in the step (1) is a high-temperature high-pressure sterilization for 10 minutes at a pressure of 1.0 kgf / cm ² at 110 ° C for 5 minutes and a pressure of 1.3 kgf / cm ² at 120 ° C A method for producing an amino acid powder of brownish dough.
The method according to claim 2, wherein the freeze-drying is carried out using a freeze drier for 48 hours until the moisture is removed, and the powder is pulverized into powder.
The method according to claim 1, wherein the protein extraction in step 2) is performed by adding 10 times the weight of the brown germ of the 30% alcohol, and extracting twice at 90 ° C for 3 hours.
6. The method according to claim 5, wherein the extraction comprises removing residues with a Wattman filter (No. 2) or centrifugation (5,000 rpm, 30 minutes) and recovering the extract. A method for producing an amino acid powder.
The method according to claim 1, wherein Protamex 0.5% (w / w) is used as the protease in step 3), and the enzyme is treated for 4 hours.
The method according to claim 1, wherein the concentration of the solid in the step 4) is a solid content of 15 to 50% at a temperature of 70 ° C or less under reduced pressure rotary evaporation using a rotary vacuum evaporator. A method for producing an amino acid powder.
The method according to claim 1, further comprising, after step (4), filtering, spray drying and granulating a seed.
10. The method according to claim 9, wherein the spray drying is performed by spray drying (200 DEG C) by adding 1,000 mL (10 times, v / w) of distilled water to 100 g of the solid, A method for producing an amino acid powder.
10. The method of claim 9, wherein the granule seed is prepared by mixing 50 g of 85.5% alcohol in a solid content of 250 g, mixing the granules with an oscillator to prepare fine granules, -0.5 &lt; RTI ID = 0.0 &gt; mm. &Lt; / RTI &gt;
The method according to claim 1, further comprising, after the step (4), a step of producing a filtration and spray concentrate.
[14] The method of claim 12, wherein the spray concentrate is prepared by mixing and agitating a volatile concentrate and an excipient extracted from a brown gruel to a concentrate having a solid content of 15 to 40%.
The method according to claim 9 or 12, further comprising, after the step, further drying in a moisture content of 5% or less and bulk packing using an aluminum pouch or a fiber drum.
14. A brown gross amino acid powder prepared by the method of any one of claims 1 to 14.