KR102386488B1 - Method for improving saponin content - Google Patents

Abstract

Disclosed is an agricultural product growing method using methylsulfonylmethane (MSM), which promotes growth of Panax ginseng and minimizes use of a chemical fertilizer by manufacturing a fertilizer containing the MSM and applying the fertilizer during production of Panax ginseng. The method for improving a saponin content of the Panax ginseng comprises the steps of: manufacturing the fertilizer containing MSM; spraying the fertilizer on soil; sowing Panax ginseng in the soil on which the spraying is completed; mixing water and the manufactured fertilizer and then fertilizing the soil at intervals of 10 to 30 days; and mixing water and the manufactured fertilizer and then spraying leaf surfaces of the Panax ginseng at intervals of 30 to 50 days.

Description

Method for improving saponin content

The present invention relates to a method for improving the saponin content, and more particularly, it can promote the growth of ginseng by preparing a fertilizer containing MSM and fertilizing it during the production of ginseng, and the saponin content that can improve the total amount of saponin It's about improvement.

MSM is a natural product produced during the oxidation process of DMSO (Dimethyl Sulfoxide). Based on its physiological action, it is formulated as food additives, pharmaceuticals, and cosmetics, including anticancer drugs, antidote, muscle pain treatment, pain reliever, antidepressant, anti-inflammatory, It is widely used as an external preparation for the skin.

The physiological actions of MSM known so far are 1) pain relief, 2) anti-inflammatory action, 3) use as a drug delivery carrier in an excellent drug delivery system, 4) vasodilation and improvement of blood flow, 5) anti-aging and intestinal peristalsis It works to improve constipation by its action and 6) to rapidly reduce scar tissue by changing the cross-linking process of collagen as a component of skin tissue.

In addition, the purified natural dietary sulfur component of MSM is a water-soluble, tasteless, odorless, white substance that is one of the most biologically safe substances.

Although MSM is a raw material that accounts for 85% of sulfur found in living organisms, it is not produced directly in the human body and can only be consumed as food. Specifically, it is found in significant amounts in mammalian milk, fresh fruits and vegetables, and the like. However, the amount of MSM contained in these natural products is too small (compared to the amount required by the human body), so it is difficult to expect the desired effect. For reference, MSM contains alfalfa 0.07ppm, corn 0.11ppm, beetroot 0.18ppm, tomato 0.2-0.86ppm, beer 0.14ppm, coffee 1.6ppm, milk 3.3ppm, tea 0.3ppm, other beets, cabbage, cucumber, oats, apples, 0.01ppm or less is detected in raspberries, etc.

To explain the effect of MSM, sulfur has an anti-inflammatory component, so it has an excellent effect on various pains of unknown cause, including arthritis, muscle and joint pain. In particular, it has a clear effect on suppression of pain caused by nerve fiber-related shock, alleviation of inflammation, activation of blood supply, alleviation of muscle spasms, and alleviation of scars.

In addition, MSM is a natural form of dietary sulfur, which forms a balance by composing joint cartilage, tendon, and ligament connective tissue, and is immediately absorbed into the body and used. That is, when supplemented with glucosamine and chondroitin, the functions of glucosamine and chondroitin can be strengthened. In addition, MSM contains sulfur, which is very important for body elements such as hormones, digestive enzymes, antibodies, and antioxidants in our cells and body proteins, and major amino acids are also affected by sulfur. In particular, there are studies showing that consuming natural dietary sulfur for knee arthritis patients can help relieve pain and improve knee function.

If the agricultural products such as vegetables we consume contain more organic sulfur, the amount of organic sulfur supplied to the human body from it will naturally increase. However, a method for increasing organic sulfur content in agricultural products has not yet been proposed.

On the other hand, the authorities that manage agriculture in Korea have been implementing policies centered on increased production based on the supply of chemical farming and cultivation technology for the past several decades. However, as a reward, it has resulted in severe ecosystem destruction due to excessive use of pesticides, soil acidification due to excessive fertilizer use, and soil degradation due to a decrease in organic matter content. In order to overcome this problem, organic farming has been promoted through the use of compost in recent years, but since the use of compost alone cannot adequately respond to pests and diseases, the proportion of organically grown crops is extremely low.

Moreover, with the inauguration of the WTO system, international competition for agricultural products is intensifying, and in particular, as a large number of cheap agricultural products are imported from neighboring countries, China, at low wages, Korean farmers will not come up with special measures such as upgrading and differentiating their products. It was difficult to survive. Due to the situation like this, the government is mobilizing all means to save our farmhouses, but so far it has not been able to come up with a sharp solution other than relying on each farmhouse's voluntary self-rescue efforts.

As seen above, our current agricultural environment has two problems, one is to overcome the degradation of rural ecosystems and soils by breaking away from chemical farming, and the other is to improve the quality and quantity of agricultural products. It is a matter of strengthening competitiveness. Although the problems of our rural areas are as clear as this, concrete proposals to solve these problems have not yet been presented. Therefore, there is a need for a new cultivation method for agricultural products to solve these problems.

(0001) Republic of Korea Patent Registration No. 10-1520296 (0002) Republic of Korea Patent No. 10-1828850

The present invention has been devised to solve the above problems, and an object of the present invention is to provide a method for improving the saponin content that can promote the growth of ginseng by applying a fertilizer containing MSM during the cultivation of ginseng. .

In addition, another object of the present invention is to provide a method for improving the saponin content by preparing a fertilizer capable of promoting the growth of ginseng, including fermented red crab by-product, seaweed extract, and silicon powder in a fertilizer containing MSM, and at the same time using the same. .

Another object of the present invention is to provide a saponin content improvement method capable of increasing the saponin content of ginseng by applying a fertilizer containing MSN during the production of ginseng.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The present invention in order to solve the above problems, MSM (MSM, Methylsulfonylmethane) comprising the steps of preparing a fertilizer; applying the fertilizer to the soil; sowing ginseng in the soil where the spraying has been completed; Mixing water and the prepared fertilizer, then adding to the soil at intervals of 10 to 30 days; And it provides a method of improving the saponin content, comprising the step of mixing water and the prepared fertilizer, and then spraying the leaf surface at intervals of 30 to 50 days.

In one embodiment, the manufacturing of the fertilizer comprises 20-50 parts by weight of chitosan extracted from red crab by-products relative to 100 parts by weight of water, 5-20 parts by weight of methylsulfonylmethane, 50-80 parts by weight of seaweed extract, and silicon preparing an MSM mixture by mixing 5 to 20 parts by weight; supplying ultrasonic waves to the MSM mixture, mixing and dispersing; After the mixing and dispersing is completed, the step of loading into a storage container and storing for 20 to 50 days; removing the solid content in the storage container after the storage is completed; and vacuum-concentrating and freeze-drying the liquid from which the solids have been removed to prepare a fertilizer.

In one embodiment, the chitosan extracted from the red crab by-product is washed with the red crab by-product, and then 200 to 300 parts by weight of water and 1 to 10 parts by weight of a proteolytic enzyme relative to 100 parts by weight of the washed red crab by-product is added. , protein removal step of reacting at a temperature of 50 ~ 70 ℃ for 10 ~ 20 hours; Inactivating the proteolytic enzyme by heating to 90 ~ 110 ℃ after the protein removal step; removing calcium by immersing in 100 to 300 parts by weight of an acidic material relative to 100 parts by weight of the red crab by-product from which the protein has been removed; Preparing chitin by immersing the red crab by-product from which the calcium has been removed in an aqueous solution of ethanol having a concentration of 3 to 5% by weight for 80 to 120 minutes; Deacetylation by mixing 100 to 300 parts by weight of the basic solution with respect to 100 parts by weight of the prepared chitin and applying ultrasonic waves; Washing with water after the deacetylation is completed to prepare chitosan; adsorbing and separating impurities by adding 200 to 300 parts by weight of water and 10 to 15 parts by weight of activated carbon relative to 100 parts by weight of the prepared chitosan; After removing the activated carbon and separating the chitosan, the separated chitosan is immersed in a solution in which 5-10 parts by weight of ascorbic acid and 5-10 parts by weight of sorbitol are mixed with 100 parts by weight of water for 5-10 days to prepare water-soluble chitosan to do; and vacuum concentration of the water-soluble chitosan, followed by freeze-drying to prepare a water-soluble chitosan powder, wherein the acidic solution is lemon juice, citron juice, sugar citron juice, orange juice and vinegar. It is a mixture of one or more selected from, the basic solution, separating the shells, and then washing; calcining the washed clam shells at a temperature of 800 to 1200 °C; pulverizing the calcined shellfish; mixing the pulverized clam shells with 1000 to 5000 parts by weight of water based on 100 parts by weight of the clam shells, and then leaving them to stand for 2 to 10 days; And it is a solution prepared by a method comprising the step of separating the solid content of the shellfish, the ultrasonic wave has a frequency of 20 ~ 130kHz, is supplied with an intensity of 300 ~ 800W, the shellfish solid content to be applied to the soil can

In one embodiment, the seaweed extract is prepared by mixing 5 to 20 parts by weight of seaweed, 20 to 40 parts by weight of seaweed, and 20 to 40 parts by weight of chlorella, based on 100 parts by weight of kelp; pulverizing the seaweed mixture and then drying; charging the dried seaweed mixture into a high-pressure chamber, and then supplying liquid nitrogen while maintaining 10 to 20° C. to pressurize the pressure inside the chamber to 5 to 10 atm and maintaining the pressure for 30 to 70 minutes; rapidly reducing the pressure in the chamber to expand the seaweed mixture in the chamber; mixing 100 to 200 parts by weight of water with respect to 100 parts by weight of the swollen seaweed mixture, then inoculating lactic acid bacteria and fermenting it for 10 to 50 days at a temperature of 20 to 30° C.; and preparing a seaweed extract by separating the seaweed solids that have subsided after the fermentation is completed.

In one embodiment, the step of adding, installing a pipe for adding at regular intervals between the sown ginseng; Connecting the fertilizer supply for additional cost to one side of the pipe for adding; supplying a fertilizer in which 5-10 parts by weight is mixed with respect to 100 parts by weight of water to the fertilizer supply unit for supplementation; And it includes the step of pressurizing the fertilizer mixed with the water every predetermined time to add between the sown ginseng through the pipe for adding, the pipe for adding is formed with holes of 0.1 to 3 mm at intervals of 10 to 100 cm it may have been

According to an embodiment of the present invention, it is possible to provide a method for improving the saponin content that can promote the growth of ginseng without the use of additional chemical fertilizers by supplying MSM, which is dietary sulfur, to ginseng.

In addition, the present invention can provide a method for improving the saponin content that can enhance the active ingredient contained in ginseng while promoting the growth of ginseng by using a mixture of MSM, red crab by-product, seaweed extract and silicon.

The effect according to the present invention is not limited by the contents exemplified above, and more various effects are included in the present invention.

1 schematically shows a ginseng cultivation method using NSM according to an embodiment of the present invention.
2 shows the chemical formula of MSM according to an embodiment of the present invention.
Figure 3 shows the appearance of a red crab according to an embodiment of the present invention.
Figure 4 shows that the red crab shells are recovered according to an embodiment of the present invention.
5 shows kelp according to an embodiment of the present invention.
6 shows that kelp is dried and processed according to an embodiment of the present invention.
7 shows a green tea according to an embodiment of the present invention.
Figure 8 shows the seaweed according to an embodiment of the present invention.
Figure 9 shows that the seaweed is dried and processed according to an embodiment of the present invention.
10 shows chlorella according to an embodiment of the present invention.
11 shows a photobioreactor for culturing chlorella according to an embodiment of the present invention.
12 is an enlarged view of chlorellar according to an embodiment of the present invention.
13 shows a clam shell according to an embodiment of the present invention.
14 shows a fertilizer mixed with water according to an embodiment of the present invention.
15 is a view showing the cultivation of lettuce according to an embodiment of the present invention.
16 shows lettuce according to an embodiment of the present invention.
Figure 17 shows the growth of lettuce according to an embodiment of the present invention.
18 shows ginseng according to an embodiment of the present invention.
19 shows ginseng cultivated for one year according to an embodiment of the present invention.
20 is a view showing the cultivation of ginseng according to an embodiment of the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described herein may be variously modified. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the accompanying drawings are only provided to facilitate understanding of the various embodiments. Therefore, the technical idea is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and scope of the invention.

Terms including an ordinal number such as 1st, 2nd, etc. may be used to describe various components, but these components are not limited by the above-mentioned terms. The above terminology is used only for the purpose of distinguishing one component from another component.

In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof. When an element is referred to as being “connected” or “connected” to another element, it is understood that it may be directly connected or connected to the other element, but other elements may exist in between. it should be On the other hand, when it is said that a certain element is "directly connected" or "directly connected" to another element, it should be understood that the other element does not exist in the middle.

Meanwhile, as used herein, a “module” or “unit” for a component performs at least one function or operation. And “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. In addition, a plurality of “modules” or a plurality of “units” other than a “module” or “unit” to be performed in specific hardware or to be executed in at least one processor may be integrated into at least one module. The singular expression includes the plural expression unless the context clearly dictates otherwise.

In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.

The present invention, the step of preparing a fertilizer containing MSM (MSM, Methylsulfonylmethane); applying the fertilizer to the soil; sowing ginseng in the soil where the spraying has been completed; Mixing water and the prepared fertilizer, then adding to the soil at intervals of 10 to 30 days; And it relates to a method for improving the saponin content comprising the step of mixing water and the prepared fertilizer, and then spraying the leaf surface at intervals of 30 to 50 days.

The MSM is a substance called methylsulfonylmethane and is generally known as dietary sulfur. This refined natural dietary sulfur component is a water-soluble, tasteless, odorless, white substance that is one of the most biologically safe substances.

Although MSM is a raw material that accounts for 85% of sulfur found in living organisms, it is not produced directly in the human body and can only be consumed as food. Specifically, it is found in large amounts in mammalian milk and fresh fruits and vegetables. However, the amount of MSM contained in these natural products is too small (compared to the amount required by the human body), so it is difficult to expect the desired effect.

For reference, MSM contains alfalfa 0.07ppm, corn 0.11ppm, beetroot 0.18ppm, tomato 0.2-0.86ppm, beer 0.14ppm, coffee 1.6ppm, milk 3.3ppm, tea 0.3ppm, other beets, cabbage, cucumber, oats, apples, Less than 0.01 ppm is detected in raspberries.

MSM is commonly used as a source of supply of sulfur, which is a component of the human body, but when supplied to plants, it can supply the sulfur components needed by plants as well as enhance the growth of microorganisms symbiotic with plants or microorganisms around plants. As a result, it can accelerate the growth of plants.

In particular, when such MSM is supplied to medicinal plants including ginseng, the growth is accelerated and necessary nutrients can be supplied in large amounts, which has the advantage of increasing the effective ingredients of plants.

Ginseng is a medicinal plant belonging to the Araliaceae family, and the Korean law defines it as "a plant belonging to the genus Ginseng of the ginseng family" (Ginseng Industry Act Article 2, No. 1). The name ginseng (人蔘) was given because the medicinal root looked just like a human. In English, it is written as ginseng, and the scientific name is 　Panax ginseng. Based on the scientific name "Panax ginseng", the fossil record is the first to be found in strata deposited in southern China in the early Cretaceous period.

When we say ginseng, we often refer to cultivated ginseng, and wild ginseng that grows in deep mountains is especially called wild ginseng. However, as a crop that was not originally cultivated, 'ginseng' in the classic Oriental medicine 　 Sanghanron (傷寒論) refers to wild ginseng.

Since 1392 　 succeeded in artificial cultivation in the Goryeo Dynasty, it has been grown well with artificial cultivation. In the case of wild ginseng, the price of wild ginseng is several hundred times higher than that of cultivated ginseng, so there is a professional collector named 'Simmani' who travels around the country to collect only old wild ginseng.

Because it is a perennial plant, it can be stored for a long time (limited to natural products), and it is said that the longer it stays, the better the medicinal properties. According to the facts revealed as 'part of research', cultivated ginseng does not differ significantly from how old it is for several years, and the content of ingredients affects it depending on the harvest time. Ginseng harvested in May and June has a slightly higher ginsenoside content. Higher.　There is a lot of misunderstanding, but the lifespan of ginseng is not very long. Fast-growing ginseng cannot live more than 6 years, and it usually rots after 7 years. Wild ginseng or wild ginseng grows longer than cultivated ginseng because it grows slowly. 100 years Most of the photos taken with a large ginseng claiming to be root are Chinese ginseng, a scam that has disappeared a lot since the certification system was implemented.

Most of ginseng uses the root, and the fruits have been used only for seedlings and have been considered useless. The red berries look just like sansuyu, but they are a bit smaller and are as bitter as ginseng.

In the past, there is a record of drying and cutting leaves and stems and selling them as tea products for brewing. According to modern research, the content of saponin-based substances, the representative component of ginseng, is higher than that of the root.

Ginseng is one of the crops that consumes a lot of intellectual power. There is even a myth that 'Ginseng can never be grown again on a land where ginseng has been cultivated once.' However, in the process of growing ginseng, a lot of fertilizer and soil are used to solve this problem, so the nutrients of the soil itself are fertile to grow other than ginseng. In fact, the biggest reason for preventing the continuous cultivation of ginseng is the disease called "ginseng root rot" caused by the pathogen Cylindrocarpondestructans, which is caused by the decomposition of ginseng residues. Since this germ can survive in the ground for about 10 years without ginseng as a host, it is treated like discarded land, and has been rotated while growing peanuts and rice instead. In modern times, technology has advanced, so either steaming the ground at all to kill germs, or spraying antagonists first to prevent the causative bacteria of root rot from propagating are also used.

In addition, in the case of the existing cultivation method, the MSM is directly fertilized in the soil or sprayed on the leaf surface of plants, so that the utilization rate of MSM is lowered. Therefore, in the present invention, in order to increase the utilization rate of MSM and to supply other types of nutrients, a fertilizer containing the MSM can be prepared and used.

To this end, the step of preparing the fertilizer includes 20-50 parts by weight of chitosan extracted from red crab by-products, 5-20 parts by weight of methylsulfonylmethane, 50-80 parts by weight of seaweed extract, and 5-20 parts by weight of silicon, relative to 100 parts by weight of water. mixing parts to prepare an MSM mixture; supplying ultrasonic waves to the MSM mixture, mixing and dispersing; After the mixing and dispersing is completed, the step of charging in a storage container and storing for 20 to 50 days; After the storage is completed, removing the solids in the storage container; and vacuum-concentrating and freeze-drying the liquid from which the solids have been removed to prepare fertilizer.

In the case of the chitosan, when fertilized during plant cultivation, the cells of the plant can be activated to promote growth, and accordingly, the harvest time of ginseng can be advanced, and when harvested at the same time as before, the size of the individual is large or effective Ginseng with a high content of ingredients can be harvested. In the case of commonly used chitosan, it is manufactured and sold as a solid that does not dissolve in water, but in the present invention, water-soluble chitosan is prepared by mixing ascorbic acid and sorbitol with chitosan prepared, and it is used for fertilizer. utilization can be increased.

In addition, the chitosan may be chitosan extracted from red crab by-products. In the case of conventional chitosan, it is generally manufactured using the shell of snow crab or blue crab, but the quantity of snow crab is limited. Therefore, in the case of the present invention, it is easy to process the shell, and it is preferable to use a by-product of red crab that is commercially available from crab meat extracted.

These red crabs belong to the long-clawed crab family, and are also called red snow crabs. It looks similar to the snow crab of the same genus, but the legs are rather short and the whole body of the red crab is crimson red.

Also, in the case of red crab, the length of the adult carapace is 6-7 cm, and the carapace forms a round triangle as a whole. The gill area is severely distended, and the back of the gills is sharply inclined. Small tubercles are scattered on the upper part of the carapace, and triangular thorns are lined up on the edge of the carapace. The forehead horn extends flat from the carapace and splits in two at the middle. Claws symmetrical and shorter than ambulatory legs. Ambulatory legs long and flat. It is morphologically similar to snow crab of the same genus, but it can be distinguished by the degree of swelling of the gill area and the shape of the forehead horn. In general, the red crab is known to inhabit at a depth of 480 to 2300 m.

In particular, in the case of red crab, compared to snow crab, it has a large number of individuals, and since it is industrially extracted and then packaged and sold, it is possible to industrially collect the by-products of the red crab. It is possible to obtain by-products with little mixing.

The red crab by-products collected as described above may be prepared and used as water-soluble chitosan. To this end, the chitosan extracted from the red crab by-product is washed with the red crab by-product, and then 200 to 300 parts by weight of water and 1 to 10 parts by weight of a proteolytic enzyme are added to 100 parts by weight of the washed red crab by-product, and then 50 to 70 ℃ Protein removal step of reacting for 10 to 20 hours at a temperature of; Inactivating the proteolytic enzyme by heating to 90 ~ 110 ℃ after the protein removal step; removing calcium by immersing in 100 to 300 parts by weight of an acidic material relative to 100 parts by weight of the red crab by-product from which the protein has been removed; preparing chitin by immersing the red crab by-product from which the calcium has been removed in an aqueous solution of ethanol having a concentration of 3 to 5% by weight for 80 to 120 minutes; Deacetylation by mixing 100 to 300 parts by weight of the basic solution with respect to 100 parts by weight of the prepared chitin and applying ultrasonic waves; Washing with water after the deacetylation is completed to prepare chitosan; adsorbing and separating impurities by adding 200 to 300 parts by weight of water and 10 to 15 parts by weight of activated carbon relative to 100 parts by weight of the prepared chitosan; After removing the activated carbon and separating the chitosan, the separated chitosan is immersed in a solution in which 5-10 parts by weight of ascorbic acid and 5-10 parts by weight of sorbitol are mixed with 100 parts by weight of water for 5-10 days to prepare water-soluble chitosan to do; and vacuum concentration of the water-soluble chitosan, and then freeze-drying to prepare a water-soluble chitosan powder.

Although the red crab by-product is commercialized by recovering the internal tissue, that is, flesh, some flesh and intestines may remain in the shell recovered as a by-product. Therefore, the red crab by-product is preferably washed with water after it is recovered and used. In addition, in this process, the red crab by-product may be cut to a predetermined size for the convenience of processing. In particular, when compression is performed to remove the leg meat sold commercially, the leg skin of the red crab maintains an elongated shape, so it not only facilitates processing by cutting it to an appropriate size, but also opens the inside of the shell for cleaning. You can do it more easily.

After the washing is completed as described above, the red crab by-product may be subjected to a protein removal step. In the case of the protein, if it is incorporated in the subsequent process as it is, decay or unwanted microorganisms may breed as a dominant species. Therefore, it is possible to remove the protein of the red crab by-product by using a proteolytic enzyme to remove the protein. In this protein removal step, it is preferable to add 200 to 300 parts by weight of water and 1 to 10 parts by weight of a protease to 100 parts by weight of red crab by-product, and then react at a temperature of 50 to 70° C. for 10 to 20 hours. If less than the above ratio of water or degrading enzyme is used, or if the reaction is performed for less than the above time, the degradation of the protein may not be complete. Costs may rise. In addition, in the case of the degrading enzyme used in the present invention, since it is an enzyme that acts in an organism, it can have maximum activity at a temperature of 20 to 40 ° C. However, in this temperature range, the reaction rate decreases and the overall reaction time is required, so 50 ~ It is preferable to make it react at the temperature of 70 degreeC. If the reaction temperature is less than 50 ℃, the reaction rate may be reduced, and a lot of time may be required for protein degradation. In addition, as the proteolytic enzyme used at this time, it is preferable to use an endo-type protease (Alcalase).

After the protein separation is completed as described above, a process of removing calcium from the red crab by-product may be performed. In the case of the calcium, it can act as a raw material included in the fertilizer, but by reacting with an acid or a base during the preparation of chitosan to decrease the reactivity, it can be difficult to produce high-purity chitosan. Therefore, in the case of the present invention, it is possible to increase the reactivity of the chitosan by removing a portion of the calcium, and thus, it is possible to manufacture high-purity chitosan.

In addition, in the case of the existing chitosan manufacturing method, the calcium is removed using hydrochloric acid, a strong acid, but when hydrochloric acid is adsorbed to chitosan and used as a fertilizer, the land may be contaminated by hydrochloric acid. can be used As the acidic solution, one or more mixtures selected from the group consisting of lemon juice, citron juice, sugar citron juice, orange juice and vinegar may be used, but since the reactivity may be lower than that of hydrochloric acid, compared to 100 parts by weight of the red crab by-product It is preferable to sufficiently dissolve the calcium present in the red crab by-product by immersing it in an acidic substance of 100 to 300, but the reaction time being 5 to 20 hours. When an acidic material is used less than the above range or when immersed for less than the above time, the elution of calcium may be incomplete, and when reacting for a time exceeding the above range or exceeding the above time, chitosan may be denatured by acid.

After the calcium is removed by the acid treatment as described above, the red crab by-product is immersed in ethanol to prepare chitin. The chitin is a major component constituting the shell of red crab, and impurities are removed through the protein separation and calcium removal, so that only chitin remains. However, even when protein and calcium are removed as described above, a carotenoid-based red pigment called astaxanthin, which is the outer pigment of red crab, may be contained. Since this pigment corresponds to a kind of polyphenol, it may have beneficial effects on the human body, but it may be difficult to absorb in plants, and may act as an impurity during chitosan production. Chitin can be prepared by removing the pigment. Specifically, when the chitin obtained by the decalcification and deproteinization treatment is immersed in an aqueous solution of 3-5 wt% ethanol at 45-55° C. for 50-70 minutes, the astaxanthin in the chitin is extracted into the solvent, and it is washed thoroughly with water Tarxanthin can be removed.

In addition, since the astaxanthin can be more easily extracted into ethanol in a basic atmosphere, it is preferable to immerse the chitin after adjusting the pH to 9 to 11 by adding a basic solution to be used in the process to be described later to the ethanol.

After the extraction of the pigment is completed as described above, the chitin may be immersed in a basic solution to undergo a deacetylation process to remove the acetyl group. Through this deacetylation process, the chitin can be converted into chitosan. This deacetylation process can be performed by mixing 100 to 300 parts by weight of a basic solution with respect to 100 parts by weight of chitin and applying ultrasonic waves. At this time, the ultrasonic wave can be used for the purpose of promoting deacetylation by generating resonance with the chitin, and through this, it is possible to lower the temperature at which the existing deacetylation process is performed from 100 to 120 °C to 80 to 100 °C, and a strong base Calcium hydroxide prepared by using clam shells can be used instead of sodium hydroxide used as

In the case of the present invention, a solution prepared using clam shells may be used as the basic solution. In the case of the deacetylation, conventionally, it was generally performed using sodium hydroxide, which is a strong base. However, a problem of soil contamination through the residual sodium hydroxide may occur, and the growth of plants may be hindered by salt generated when the sodium hydroxide is neutralized. In the present invention, calcium carbonate produced using shellfish and a solution containing calcium hydroxide produced by dissolving the calcium carbonate in water.

This basic solution is separated from the clam shells, followed by washing; calcining the washed clam shells at a temperature of 800 to 1200 °C; pulverizing the calcined shellfish; mixing the pulverized clam shells with 1000 to 5000 parts by weight of water based on 100 parts by weight of the clam shells, and then leaving them to stand for 2 to 10 days; And it may be a solution prepared by a method comprising the step of separating the solid content of the shellfish.

The shell of the clam is composed of a large amount of calcium carbonate as a main component, and most of it is treated as waste. Therefore, when calcium carbonate is extracted from these shells and used, desired effects can be exhibited while contributing to resource recycling and environmental protection. The clam shells may be washed and organic matter removed, and then calcined at a temperature of 800 to 1200 °C. Through this, organic matter present in the shell may be oxidized and removed, and only calcium carbonate may remain. At this time, if the firing temperature is less than 800 ℃, smooth firing may not be performed, and if it exceeds 1200° C., energy consumption may increase as well as calcium carbonate may be decomposed by calcination, thereby reducing the yield.

In the present invention, various shells may be used as the shells, but it is more preferable to use oysters that produce a large amount of shells.

It is a type of mollusk that belongs to the bivalve family 　 oyster family and is nutrient-rich and is consumed in various regions in both East and West.

Oysters live close to shore on rocks. The oysters that are widely consumed worldwide are 　Crassostrea and 　Ostrea.

When we think of oysters, what we usually think of is Crassostrea gigas (pacific oyster), which is widespread in the East Asian coast, and is the most consumed type worldwide.

On the other hand, oysters in Ostrea are characterized by their round and flat appearance. There are 'European flat oysters' (O. edulis) traditionally consumed in the Atlantic region, 'crypt oysters' (O. denselamellosa) and native oysters (O. circumpicta) found in the Yellow Coast of East Asia.

Wild oysters and farmed oysters are easy to distinguish by their appearance. The natural mountain has a thin and wavy shell so that it is not washed away by waves while repeating the process of being submerged in seawater and exposed to air. Because cultured oysters stay in the sea, they grow round and large. It is easy to eat because of its large size, but it is slightly wrong to say that the natural taste is thicker, and it depends on the region where it is grown. This is because aquaculture on the west coast is raised in tidal flats or in places with severe tidal differences, so it is immersed in seawater and exposed to the air.

Oyster season is from mid-September to April of the following year, and the spawning season is from May to August. Since ancient times, it has been said in Asia that 'you must not eat oysters when the barley is blooming', and in Europe and the United States, it is said that you should not eat oysters during the four months of May to August, when the Latin letter R is not included. If the water temperature is higher than a certain temperature, it will taste like a potato that has sprouted from paralytic shellfish poison, and if consumed in excess, it may cause breathing difficulties or even death. Typical shellfish poisons include saxitoxin and benelupine, of which oysters have a higher content of benelupine.

Also, in years with frequent typhoons, a lot of floating matter on land comes down to the sea through heavy rain and wind, which can be a good thing for oyster and aquaculture.

These oysters are called the milk of the sea, and are considered as one of the delicacies with high nutritional value and rich taste, as well as high-quality seafood.

Oysters are rich in zinc, and the zinc component promotes the secretion of testosterone, the male hormone, and helps with sperm production and activity. However, it is said that there is a recovery effect when the level of 　hormone and 　 is below the normal range, and it has no meaning for people who are already normal. This is because the body suppresses excessive secretion in order to maintain homeostasis. One of the most misconceptions about energy related foods. As will be described later, it seems that the famous 　Casanova enjoyed eating oysters as one of the secrets of maintaining energy even in old age, so it seems to have become a food symbolizing energy.

Also, as the saying goes, 'The face of a fisherman's daughter is black and the face of a fisherman's daughter is white', it has the effect of decomposing melanin pigment and is good for skin care.

There are also claims that it is a good food to prevent hair loss. It seems that the zinc contained in oysters not only promotes spermatogenesis but also inhibits the production of DHT hormone, which is the cause of hair loss. In addition, there are studies that it is good for curing colds.

These oysters lose microscopic levels of nutrients when heated. Only a small amount of vitamin is lost, but protein and minerals, which are the main components of oysters, are not damaged. Therefore, even when heated, there is no significant nutritional loss. In addition, raw food has a low digestibility and absorption rate, so it is an inefficient intake method, so it may be better to heat it up and eat it.

After the calcination is completed as described above, it can be pulverized, and mixed with 1000 to 5000 parts by weight of water based on 100 parts by weight, and then left for 2 to 10 days to prepare an aqueous calcium carbonate solution. At this time, when water is used in less than 1000 parts by weight, the amount of the aqueous solution to be prepared is reduced and the efficiency may be reduced. .

In addition, since the residue generated in the above process, that is, the shell residue, contains a large amount of calcium and minerals, it may be applied to the soil and used as an auxiliary material for the fertilizer of the present invention.

In addition, the ultrasonic wave used in the deacetylation process has a frequency of 20 to 130 kHz, and may be supplied at an intensity of 300 to 800 W. When the ultrasonic wave is out of the frequency range, it cannot cause a resonance phenomenon with chitin, and thus deacetylation efficiency may be reduced. In addition, when supplied with an intensity exceeding 800W, the decomposition strength of chitin becomes strong, so that the molecular structure of chitin can be decomposed, and thus the extraction efficiency may be reduced.

In addition, it is usually defined as chitosan when 70% or more of the acetyl group of chitin has been removed. Factors that influence the physical properties and functionality of chitosan are viscosity and degree of deacetylation, and the viscosity of chitosan depends on the molecular weight of chitosan.

Chitosan produced through the deacetylation process as described above may be prepared in a solid form. Therefore, the prepared chitosan can be immersed in water containing 5 to 10% by weight of ascorbic acid and 5 to 10% by weight of sorbitol for 5 to 10 days so that the chitosan has water solubility.

Ascorbic acid is dissociated in water and separated into a cation and anion. The cation of ascorbic acid interacts with the amine group of chitosan to charge the amine group to a cation. By forming ionic bonds with ions, it has a stable structure and chitosan has water solubility.

The water-soluble chitosan prepared as described above may be used after being vacuum-concentrated for convenience of storage and then freeze-dried to prepare a water-soluble chitosan powder.

In the case of the present invention, in addition to the red crab chitosan powder, a seaweed extract may be used. The seaweed contains a large amount of trace elements including minerals necessary for plant growth, and contains various metal salts, so it can assist in normal plant growth despite acidification of the soil.

To this end, the seaweed extract is prepared by mixing 5-20 parts by weight of seaweed, 20-40 parts by weight of seaweed, and 20-40 parts by weight of chlorella, based on 100 parts by weight of kelp; pulverizing the seaweed mixture and then drying; charging the dried seaweed mixture into a high-pressure chamber, and then supplying liquid nitrogen while maintaining 10 to 20° C. to pressurize the pressure inside the chamber to 5 to 10 atm and maintaining the pressure for 30 to 70 minutes; rapidly reducing the pressure in the chamber to expand the seaweed mixture in the chamber; mixing 100 to 200 parts by weight of water with respect to 100 parts by weight of the swollen seaweed mixture, then inoculating lactic acid bacteria and fermenting it for 10 to 50 days at a temperature of 20 to 30° C.; and preparing a seaweed extract by separating the seaweed solids that have subsided after the fermentation is completed.

The seaweed used in the present invention is mixed with 100 parts by weight of kelp, 5-20 parts by weight of seaweed, 20-40 parts by weight of seaweed, and 20-40 parts by weight of chlorella in order to supply various minerals and nutrients and at the same time reduce the manufacturing cost. can be used by Of these, in the case of kelp and laver, the remains of processing kelp and laver collected for food can be used. In addition, in the case of chlorella, it is possible to use chlorella that grows wild in the sea, but it is also possible to use chlorella that is harvested from a photobioreactor that has been widely used recently. In addition, in the case of such seaweed, it is preferable to be mixed in a ratio within the above range. It can be manufactured at a low cost within the above range, and can have optimal efficiency for plant growth, but when it is out of the above range, the manufacturing cost may increase or the effect of increasing the growth of the plant may be reduced.

The kelp is a seaweed belonging to the kelp family of the kelp family, and it attaches to rocks and reproduces as spores. It is characterized by having thick leaves with a thickness of 2-3 mm, and inhabits only in boreal and boreal coasts. It is said that it inhabits mainly in the northern part of the east coast, 　Hokkaido, 　Tohoku, 　Coast, 　Kamchatka Peninsula, 　Sakhalin and the islands. Originally, it did not inhabit in the central and southern seas of Korea, but it is produced in large quantities from the east coast to the south coast, and Jeollanam-do, centered on Wando, accounts for most of the total production. Geographical indication system / In Korea, 　Gijang-gun, 　Wando-gun, 　Goheung-gun 　kelp are registered.

In particular, in 　 Korea and 　 Japan, 　 Sakhalin, where the surrounding area is all over the sea, 　 seaweed and seaweed are eaten as much as 　 seaweed, 　 seaweed, and it is cheap and easy to obtain. In particular, because of its unique 　 umami taste, it is often dried and used for soup stock. In addition, they are eaten raw, or fried kelp dipped in sugar, and there are various types of it, which are seasoned and then pressed and semi-dried to make jelly. In the United States and the United States, it seems that seaweed was originally a fairly unfamiliar food, but these days, recipes for adding it to salads are being developed. In France and other countries, various recipes are being tried, including butter with seaweed such as kelp.

The green tea is an annual plant that grows on the beach. As can be seen from the scientific name, it is mainly distributed and used in Europe, and even the scientific name was given by Linne, who started taxonomy. It is mainly distributed in the cold regions, and in addition to Europe, it also inhabits North America and Northeast Asia. In Korea, it also occurs in 　 North Jeolla Province, 　 Gyeonggi-do, 　 Pyeongan-do, 　 Hamgyeong-do 　 Coast. Hamcho (鹹草) is often used because it tastes salty. The flower blooms from August to September, and it is said to grow up to 35cm when it grows well. Originally, it was green, but in autumn, it is dyed red. It turns pink in early autumn and turns reddish brown in late autumn.

Among plants, green tea is uniquely found mainly in swampy areas with high salinity, that is, in tidal flats. The reason it is rarely found on the east coast of Korea is that it cannot grow on sandy soil. Also, since it is a plant that needs salt for physiological phenomena, it has abundant salt throughout the individual. Taking advantage of these characteristics 　 in Korea, it is collected and ground 　 as a substitute for salt 　, and by squeezing the juice 　 as a substitute for soy sauce. It's salty anyway, so it can be used without too much trouble for seasoning, and the main base is salty and the other flavors are not too big, so it doesn't spoil the taste. One of the most popular dishes is the seasoned pork ribs called Hamcho Galbi.

The British simply eat this. In fact, if you think about how precious salt was in the pre-modern era, it seems that this salt-rich plant was consumed as a substitute for salt. Although it is eaten raw in Europe outside the UK, it is also used for cooking on the same ship as asparagus. In the English-speaking world, the popular name is 'Glassvort'. It seems that it was because the sodium carbonate needed to make glass was obtained from burnt ash until chemically mass-produced.

Strictly speaking, the above seaweed is a generic term for seaweed belonging to the genus Purple and laver. It has been known for a long time that seaweed belongs to the supergroup Archaeaplastida Rhodophyte, but according to recent research results, the protochromophyll system forms a paraphyletic group rather than a monophyletic group, and seaweed It has become orthodox that the red algae plants, including Glaucocystophyte, belong to the plant kingdom along with Chlorophyte. Evidence　 However, the journal was published in 2010.

According to more recent classifications such as 2012, 2014, and 2016, green plants (Viridiplantae), Rhodophyta, Glaucophyta, etc. are subordinate to Archaeplastida. There is a plant kingdom under Viridiplantae, and only Chlorophyta, Chaophyta, Embryophyta, etc. belong to Plantae. That is, as the plant kingdom itself entered the subgroup of the Chlorophyte of the Supergroup Archaeaplastida, seaweed still belongs to the Rhodophyte of the Supergroup Archaeaplastida.

For reference, the protochromozoites were differentiated approximately 1.5 billion years ago, and the posterior flagellums were differentiated approximately 1.3 billion years ago, so there is a greater biological difference between the green algae green algae and the red algae seaweed, the fungus, the animal type, and humans. .

There are various species of red algae, about 6,000 species are known. Red algae include phycoerythrin, which is one of the pigments that efficiently collects light necessary for photosynthesis. This pigment mainly absorbs blue and green light among the wavelengths of light. Not all reddish plants have a red color, and the color varies from species to species, even for individuals of the same species. It has to do with the fact that the wavelengths of light are different. Because blue light reaches the deepest depths of water, red plants that live in very shallow places are greenish-red, those that live a little deeper are bright red, and those that live deeper than this are almost black. takes on Furthermore, some species lose their photosynthetic ability and live as a parasite on other organisms.

It has been confirmed that those living in the deepest depths live at a depth of 260 m. Many red-flowered plants are multicellular, but none have been found as long as kelps in the sea. Reddish plants also disseminate their offspring through a unique and unique reproductive method called diplohaplontic or triphasic. do.

Such seaweed is known to be rich in iodine. Also, it is the only plant food that is very rich in cyanocobalamine (vitamin B12). Among plant foods, it belongs to the axis with high protein content.

The chlorella is an oval, single-celled, and green alga of a fine size. They are not motile at all, and reproduce through the primitive method of individual division. Because it is a single cell, it is impossible to identify an individual with the naked eye, and it can only be identified using an optical microscope among microscopes. (The existence of chlorophyll can be determined by the presence or absence of chlorophyll due to the characteristics of microalgae.) So far, about 10 species have been discovered, and their photosynthetic ability is excellent, so their growth is fast and proliferation is fast. Under the right conditions, it can multiply 10 times a day.

Compared with the same amount of rice, the amount of organic matter that can be produced in one year is 8 times. In addition, it is a complete food that contains all three major energy sources, 　 carbohydrates, 　 proteins, and 　 fats, as well as 　 vitamins and fiber 　 in cells. Since the content can be adjusted according to the culture conditions, it is recognized as a substitute food for the future. If there is a problem, it is too small, the digestibility and absorption rate is low, and above all, it has the disadvantage that it is tasteless. Most of them are so tasteless that they cannot be eaten while savoring the taste, so they simply swallow the concentrated capsule with water.

Chlorella, which improved digestion and absorption through aseptic culture technology and cancer culture technology, appeared, and it was often sold as a health food. In Korea, the chlorella released by Daesang Group is famous for its health food. The advertisement is talking about amino acids, multi-vitamins, antioxidants, skin beauty ingredients, etc. In fact, it is close to exaggerated advertising. It is not without it, but it is because the amount is too small and the absorption rate is small. In any case, thanks to the effectiveness of advertising, it boasts the same status as a comprehensive nutritional supplement.

In addition, it is used as a nutritional food, and is used in various fields such as 　 microbial 　 growth promotion, coloring, and water purification.

As the development progressed gradually, chlorella, which tasted good enough to eat, was also developed. When you chew it, you can feel the texture like moist laver and the tasteless smell, but this was developed to a level that is edible. The whole mouth is stained green, and even if you brush your teeth, the water doesn't drain well. According to an experienced person, the water does not drain out even after brushing 5 times, and the toothbrush has turned green.

If eaten raw, most of the granules or pills are swallowed without chewing. Otherwise, it is more often used as an additive.

Thanks to these strong dyeing properties, it is playing an active role as a natural food colorant. Most of the food that contains green is due to chlorella. It was green tea food that benefited the most. Because it can show the green color clearly, I made sure to make the image that green tea is in it. Most foods such as green tea noodles are also colored with chlorella rather than green tea. Chlorella itself is not good or bad if it is good for the body, but the problem is that the vivid color is falsely attributed to green tea.

The seaweed mixed in the above ratio may be pulverized and then dried for a swelling process to be described later. At this time, it is preferable that the pulverization be pulverized so that the average particle diameter is 1 to 5 mm, and the pulverized seaweed is placed on a porous tray and dried for 3 to 10 days so that the moisture content is less than 10% by weight.

The dried seaweed mixture as described above may be subjected to a swelling process. In the case of the conventional expansion process, it is heated to a high temperature to form a high-pressure condition, and then, the moisture in the expansion object is vaporized through rapid pressure reduction. However, in the case of swelling through such heating, active ingredients included in the seaweed mixture may be thermally decomposed, and the surface of the seaweed may be hardened when heated, making the extraction process to be described later more difficult.

Therefore, in the case of the present invention, heat damage and curing due to heating can be minimized by pressurizing using an inert gas and then rapidly reducing the pressure in place of the heating-type swelling process.

To this end, the dried seaweed mixture may be charged into the high-pressure chamber, and after the charging is completed, the chamber may be closed and liquid nitrogen may be injected. In this process, in order to pressurize the inside of the chamber, the inside of the chamber may be maintained at 10-20°C. Through this, the inside of the chamber can be pressurized to 5 to 10 atmospheres. When the temperature and pressure are less than the three ranges, it is difficult to perform normal expansion, and when it exceeds the above ranges, the manufacturing cost and operating cost of the chamber may increase due to excessive high pressure. In addition, in this process, a portion of the supplied liquid nitrogen may be introduced into the seaweed mixture, and the liquid nitrogen introduced in this way is vaporized in a swelling process to be described later to expand the seaweed mixture.

The pressurization may be maintained for 30 to 70 minutes. In the pressurization process, a certain time is required to increase the pressure inside the seaweed mixture. In particular, since the seaweed has a cell wall similar to that of a plant, the expansion of the seaweed can be smoothly performed by maintaining the same time as described above.

After maintaining the pressure for a predetermined time as described above, the chamber may be rapidly depressurized to expand the seaweed mixture. In this process, the liquid nitrogen introduced into the seaweed mixture and the gas and moisture inside the seaweed mixture may be vaporized and expanded, and through this, it is possible to expand the seaweed mixture similarly to the conventional heating and expansion method.

As described above, the seaweed mixture, whose swelling has been slowed down, becomes porous in the swelling process, which can smoothly perform the extraction process to be described later. In particular, part of the cell wall of the seaweed is damaged during the expansion process, so that the extraction of the active ingredient can be performed more easily.

As described above, the seaweed extract that has been completely expanded can be extracted by contacting the lactic acid bacteria. In the case of the lactic acid bacteria, acidic substances may be discharged while being cultured. Therefore, it is possible to decompose the surface of the seaweed mixture to extract the active ingredient contained therein. At this time, the active ingredient can be extracted even when the lactic acid bacteria are not inoculated, but in this case, the microorganisms prosper and deteriorate or decay. can be easily extracted.

The lactic acid bacteria are gram-positive bacteria that mainly produce lactic acid as a result of fermentation, and are also called lactic acid bacteria. Broadly speaking, it is a generic term for bacteria of the genus Lactobacillus and Lactobacillus of the order Lactobacillus, and in a narrow sense, it refers to beneficial bacteria that coexist with humans in the intestines, oral cavity, and genitals of the person. Its existence and efficacy were first known by Russian scientist Ilya Mechnikov.

These lactic acid bacteria are beneficial to the human body and health. They prevent the growth of harmful bacteria in the intestine and regulate immunity to normal levels, improve autoimmune skin diseases such as atopy and psoriasis, suppress high blood pressure and metabolic syndrome, lower insulin resistance, etc. It is known that there is Also, there are research results showing that while in the intestine, it is involved in neurological substances in the brain, helping to some extent in depression. A study was also published that secretes a polysaccharide called 'Rhamnose' and secretes cytokines and chemokines, substances that induce an immune response in the body against it.

Lactobacillus is generally known to be beneficial to gut health. This is due to the characteristics of lactic acid bacteria, which have the characteristic of secreting lactic acid (lactic acid) to kill other bacteria, hence the name 'lactic acid bacteria'. But the interesting thing is that if this state continues and the acidity becomes too high, they die from the lactic acid they secrete. What you can see in real life is fresh kimchi, which means that when the kimchi becomes sour, a lot of lactic acid bacteria proliferate. means So, it is said that kimchi is the best for the body right before the military smell. However, there is a saying that there are more lactic acid bacteria in the kimchi broth than the kimchi itself.

In the past, there was a theory that the lactic acid bacteria in yogurt could be the cause of tooth decay, but the current consensus is that it has nothing to do with tooth decay. The key cause of tooth decay is lactic acid (lactic acid) secreted by S. Mutans. However, mutans is not a lactic acid bacteria, but a type of streptococci. The mutans group and lactic acid bacteria (Lactobacillus) belong to the order Lactobacillus, both of which secrete lactic acid.

Today, microbes in the body are considered to be as important as being found to affect health as much as genes. Our bodies are bacteria that reside in our gut (mostly in the small and large intestine), and our bodies are It is affected by the products secreted by those bacteria. Therefore, lactic acid bacteria have an effect on our health, especially on immune regulation and infection. Breast milk, organic, preservative-free, antibiotic-free, fermented food, fresh food, slow food, any food, anti-inflammatory food, vegetarian, raw food, appropriate spices, warm water, sour food, Actions such as fresh food, oligosaccharides, non-stress, and periodic replacement of toothbrushes actually affect the composition of bacteria in the body until they have an effect on our health, and as a result, health is improved by securing beneficial bacteria. In fact, human beings can sustain life with only water, vitamins, proteins, carbohydrates, fats, minerals and adequate fiber nutritionally, but bacteria cannot be overlooked. The world is full of germs, and our bodies are also home to germs. In terms of bacteria, they are greatly affected by the environment and the number of individuals is also controlled in real time. Independently, intestinal bacteria have favorable conditions for the environment in our body, and when the number of individuals increases to a certain level, they are also involved in the metabolism and health of our body. Moreover, some cells block external pathogens and prevent infection. In the case of lactic acid bacteria settled in a healthy state together with various beneficial bacteria, it maintains the health of the whole body.

These lactic acid bacteria are abundantly contained in fermented foods, such as yogurt and kimchi. It is easy to think that yogurt is made from milk and eaten, but if you know it, you can make any number of vegetable yogurts. It is often made with soy milk, almonds, and milk. Powder/capsule type lactic acid bacteria products are also sold at pharmacies, supermarkets, and online. In addition, in order to increase the effectiveness of the lactobacilli taken, FOS (fructooligosaccharide) supplements, which are the energy of intestinal lactic acid bacteria, are sometimes taken together, but they are usually included in lactic acid bacteria products.

The lactic acid bacteria refers to only Lactobacillus among probiotics, which are living non-pathogenic microorganisms beneficial to the human body, but in Korea, all probiotics including Bifidobacterium are called lactic acid bacteria. In the past, it was thought to be a type of Bifidobacterium dolactobacillus.

Representative lactic acid bacteria include Lactobacillus casei (L.casei), Lactobacillus acidophilus (L.acidophilus), Lactobacillus bulgaricus (L.bulgaricus), Bifidobacterium longum (B.longum), Bifidobacterium Bifidum (B. bifidum), Actiregularis (Actiregularis), Lactobacillus rhamnosus (L.rhamnosus) [18] 　 and the like. In addition to this, various types of lactic acid bacteria exist.

The lactic acid bacteria used at this time can be used without limitation as long as they are commercially available lactic acid bacteria, but preferably Lactobacillus plantarum, Lactobacillus Sake, Lactobacillus reuteri, Lactobacillus rhamnosus or mixtures thereof may be used.

After mixing with 100 to 200 parts by weight of water based on 100 parts by weight of the swollen seaweed mixture, lactic acid bacteria can be inoculated and fermented at a temperature of 20 to 30° C. for 10 to 50 days. Under the conditions within the above range, fermentation by mammary gland bacteria is easy, but if it exceeds the above range, it is not fermented and the extraction efficiency is reduced, or other types of microorganisms may grow and cause off-flavor.

In addition, in the extraction process, an oligosaccharide may be mixed to further promote the culture of the lactic acid bacteria. The oligosaccharide is a material serving as food for lactic acid bacteria to be described later. In the present invention, lactic acid bacteria are used for extraction, but when used in a mixture in the above ratio, sugar components for culturing lactic acid bacteria may be insufficient. Therefore, it is preferable to mix the oligosaccharides to create an environment suitable for culturing lactic acid bacteria. In this case, when the oligosaccharide is included in less than 1 part by weight, it cannot have a sugar concentration for culturing lactic acid bacteria, and when it is added in excess of 5 parts by weight, it may be difficult to prepare a liquid fertilizer when used due to excessive oligosaccharides.

After the fermentation by lactic acid bacteria is completed as described above, the seaweed extract can be prepared by separating the solids that have settled to the bottom of the container.

An MSM mixture can be prepared by mixing 20-50 parts by weight of chitosan extracted from red crab by-products relative to 100 parts by weight of water, 5-20 parts by weight of methylsulfonylmethane, 50-80 parts by weight of seaweed extract, and 5-20 parts by weight of silicon. there is. The chitosan and seaweed extracts used at this time are as described above, and in the case of methylsulfonylmethane and silicon, it is preferable to use those sold for fertilizers or those sold for reagents. In addition, within the above range, the fertilizer may exert a normal effect, but when used in a ratio outside the above range, the manufacturing cost of the fertilizer may increase or the growth rate of ginseng may decrease.

It is preferable to supply ultrasonic waves to the MSM mixture to mix and disperse. In the case of the raw material used in the present invention, it can be easily mixed with water, but by applying ultrasonic vibration during mixing, the mixing of the above components can be formed more uniformly. It facilitates mixing at a high speed.

After the mixing is completed as described above, it can be charged into a storage container and stored for 20 to 50 days. In this storage process, unmixed solids may sink to the bottom, and precipitates generated by side reactions occurring between each component may also be separated. After the storage is completed as described above, the solids are removed, and the fertilizer can be prepared by vacuum concentration and freeze-drying.

The fertilizer prepared as described above may be applied to the soil and ginseng in three steps. First, before sowing ginseng in the soil, it is possible to increase the active ingredients of the soil by applying the fertilizer. At this time, the amount of fertilizer applied is 100 to 200 g per 1000 kg of topsoil or 10 to 20 g per 1 ㎡ of farmland, which can be adjusted appropriately depending on the type of ginseng and the surrounding environment. When fertilizing less than the above range, it is difficult to expect the effect of the fertilizer, and when fertilizing in an amount exceeding the above range, the growth of ginseng is accelerated too much, and the marketability may be deteriorated or vulnerable to pests and diseases.

In addition, after the ginseng is sown, it may be additionally fertilized or fertilized. The fertilization of the fertilizer before sowing of the ginseng serves to increase the active ingredients in the soil, but these active ingredients are absorbed and consumed by the ginseng and may be lost by the rain at the same time. Therefore, in order to properly maintain the concentration of these active ingredients, it is possible to increase the effectiveness of the fertilizer by appropriately fertilizing even after sowing of ginseng.

To this end, the step of fertilizing may include: installing pipes for adding at regular intervals between the sown ginseng; Connecting the fertilizer supply for additional cost to one side of the pipe for adding; supplying a fertilizer in which 5-10 parts by weight is mixed with respect to 100 parts by weight of water to the fertilizer supply unit for supplementation; And pressurizing the fertilizer mixed with the water every predetermined time to add to the planted ginseng through the pipe for adding, wherein the pipe for adding is formed with holes of 0.1 to 3 mm at intervals of 10 to 100 cm it may have been

In the case of the additional fertilization, it is also possible to fertilize using manpower, but it has the disadvantage that it is difficult to fertilize uniformly as well as require a lot of manpower. Therefore, it is possible to evenly fertilize while reducing the use of manpower by using a pipe for additional fertilization so that such fertilization can be carried out automatically. At this time, the supplementary tube may be installed at regular intervals between the ginseng, and preferably may be installed to have an interval of 30 to 200 cm. When the supplementary tube has an interval of less than 30cm, the space required for the growth of ginseng may be reduced, and if it has a spacing exceeding 200cm, it may be difficult to uniformly supply fertilizer for supplementation.

In addition, holes of 0.1 to 3mm may be formed in the pipe for adding at intervals of 10 to 100cm. The hole is a part through which the fertilizer supplied through the pipe for addition is ejected, and a hole may be simply installed in the pipe, but a nozzle may be installed in the hole for more uniform addition. In addition, if the distance between the holes is less than the above range or exceeds the diameter, excessive fertilizer may be supplied, and if the hole exceeds the above range or less than the diameter, the supply of fertilizer may not be smooth.

In addition, the supplementary fertilizer may be supplied by mixing in a ratio of 5 to 10 parts by weight compared to 100 parts by weight of water. Through this, moisture and fertilizer can be supplied to ginseng at the same time, and the fertilizer for supplementation can be uniformly supplied to a longer distance by using the flow of water supplied at the same time. When mixed in less than the above ratio, excessive water may be mixed to reduce the fertilization effect, and if mixed in a ratio exceeding the above range, the concentration of the fertilizer may be increased and the effect may be decreased.

In addition, the pipe for adding is generally used for the cost of adding fertilizer as described above, but it can also be used for supply of water, or supply of additional fertilizer.

One side of the pipe for adding may be connected to a fertilizer supply for additional cost. The supplementary fertilizer supply unit may be configured with a storage tank and a pressurization unit that can store the supplementary fertilizer to be installed to supply the supplementary fertilizer to the supplementary pipe. That is, the fertilizer and water are mixed in an appropriate ratio and injected into the storage tank, and then the pressurizing unit is operated to pressurize the supplementary fertilizer in the storage tank and supply it to the supplementary pipe. It may be sprayed to the outside through a hole formed in the pipe for adding. In addition, a controller may be connected to the pressing unit to automatically perform this additional fertilization, and the controller may perform the additional fertilization at appropriate time intervals by operating and stopping the pressing part at a specified time. In this case, the fertilization may be performed at intervals of 10 to 30 days. When the additional fertilization is carried out at intervals of less than 10 days, excessive fertilizer may be supplied.

In addition, the fertilizer may be applied to the leaf surface of ginseng separately from the initial fertilization and additional fertilization. In the case of the fertilization and fertilization, as the fertilizer is supplied to the soil, the fertilizer can be absorbed through the roots and simultaneously absorb the components of the soil, but the disadvantage is that the amount absorbed is small compared to the amount of fertilizer used. Have. Therefore, in order to compensate for this, it is possible to increase the effect of the fertilizer by spraying the fertilizer on the leaf surface at intervals of 30 to 50 days. In this case, in the case of fertilizer applied to the leaf surface, 20 to 40 parts by weight of the fertilizer can be mixed with respect to 100 parts by weight of water. If the spraying interval is less than the above period or the mixing ratio exceeds 40 parts by weight, an excessive amount of fertilizer may be supplied, and when the interval is exceeded or mixed in a ratio of less than 20 parts by weight, the effectiveness of the fertilizer may be reduced.

Hereinafter, preferred embodiments of the present invention will be described so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. In addition, in the description of the present invention, when it is determined that a detailed description of a related known function or a known configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, certain features presented in the drawings are enlarged, reduced, or simplified for ease of explanation, and the drawings and components thereof are not necessarily drawn to scale. However, those skilled in the art will readily appreciate these details.

Example 1

The red crab by-product collected in Gangneung-si, Gangwon-do was cut to a size of 3 cm, washed, mixed in a ratio of 250 parts by weight of water and 5 parts by weight of protease to 100 parts by weight of the red crab by-product, and reacted at a temperature of 60° C. for 12 hours to make protein was removed. After the protein removal step, the protease was inactivated by heating at 100° C. for 20 minutes.

An acidic substance was prepared by mixing 100 parts by weight of water in a ratio of 50 parts by weight of lemon juice, and calcium was removed by immersion in 200 parts by weight of an acidic substance compared to 100 parts by weight of the red crab by-product from which the protein was removed for 12 hours.

Chitin was prepared by immersing the red crab by-product from which the calcium was removed in an aqueous solution of ethanol having a concentration of 3 to 5% by weight for 100 minutes.

After washing the oyster shells collected in Namhae, the washed oyster shells were calcined at a temperature of 1000°C. The calcined oyster shells were pulverized and mixed with 2000 parts by weight of water based on 100 parts by weight of the pulverized oyster shells, and then left to stand for 7 days. After the standing was completed, the oyster shell solid was removed to prepare a basic solution.

200 parts by weight of the basic solution with respect to 100 parts by weight of the prepared chitin was mixed and deacetylated by applying ultrasonic waves. At this time, ultrasonic waves had a frequency of 90 kHz and were supplied at an intensity of 600 W for 10 minutes. After the deacetylation was completed, chitosan was prepared by washing with water.

Impurities were adsorbed and separated by adding 200 to 300 parts by weight of water and 10 to 15 parts by weight of activated carbon relative to 100 parts by weight of the chitosan, removing the activated carbon and separating chitosan, and then adding the separated chitosan to 100 parts by weight of water Water-soluble chitosan was prepared by immersion in a mixed solution of 7 parts by weight of ascorbic acid and 6 parts by weight of sorbitol for 7 days. The water-soluble chitosan prepared as described above was prepared as chitosan powder through vacuum concentration and freeze-drying process.

A seaweed mixture was prepared by mixing 100 parts by weight of kelp, 10 parts by weight of seaweed, 30 parts by weight of seaweed, and 30 parts by weight of chlorella, followed by pulverization and drying. The dried seaweed mixture was charged into a high-pressure chamber, and then liquid nitrogen was supplied while maintaining 15° C. to pressurize the pressure inside the chamber to 8 atmospheres and maintained for 60 minutes. After 60 minutes had elapsed, the pressure in the chamber was rapidly reduced to expand the seaweed mixture in the chamber. After mixing with 150 parts by weight of water based on 100 parts by weight of the swollen seaweed mixture, lactic acid bacteria were inoculated and fermented at a temperature of 25° C. for 20 days. After the fermentation was completed, the seaweed extract was prepared by separating the sunken seaweed solids.

An MSM mixture was prepared by mixing 40 parts by weight of chitosan, 15 parts by weight of methylsulfonylmethane, 60 parts by weight of seaweed extract and 10 parts by weight of silicon, relative to 100 parts by weight of water. Ultrasound was supplied to the MSM mixture, mixed and dispersed, and after the mixing and dispersion was completed, it was charged into a storage container and stored for 30 days. After the storage was completed, the solid content in the storage container was removed, and the liquid was vacuum-concentrated and freeze-dried to prepare a fertilizer.

Example 2

The same procedure was performed except that MSM was not used in Example 1.

Example 3

The same procedure was performed except that water-soluble chitosan prepared using red crab in Example 1 was not used.

Example 4

In the preparation of the water-soluble chitosan, hydrochloric acid as an acidic solution and sodium hydroxide aqueous solution as a basic solution were used in the same manner as in the conventional method, except that the water-soluble chitosan solution was used.

Example 5

The same procedure was performed except that the seaweed extract was not used in Example 1.

Example 6

The same procedure was performed in Example 1, except that the conventional heating-type swelling method was used instead of the swelling method using an inert gas when the seaweed extract was prepared.

Experimental Example 1

An experiment was conducted to confirm the plant growth effect by the fertilizer and the cultivation method according to the present invention.

15 g of the fertilizer prepared by the method of Examples 1 to 6 was fertilized in a field partitioned to a size of 1 m 2 , and lettuce was sown at an interval of 10 cm. After the sowing was completed, cultivation was performed while maintaining appropriate humidity, and fertilizer solution was sprayed on the leaf surface at intervals of 15 days and 40 days, followed by cultivation for 100 days.

After the completion of 100 days, the average height of the whole herb, the number of leaves, and the length of the leaves were measured and averaged and shown in Table 1 below.

Height (cm) Number of leaves (pieces) Leaf size (cm) Example 1 38 13 18 Example 2 22 8 8 Example 3 26 10 11 Example 4 24 10 8 Example 5 29 11 9 Example 6 31 11 15

As shown in Table 1, Example 1 of the present invention showed the highest growth rate. Example 2 in which MSM was not used showed the most unfamiliar growth rate, and showed a lower growth rate compared to Example 1 of the present invention even when the remaining ingredients were not used or prepared by the conventional method.

Experimental Example 2

An experiment was conducted to confirm the effect of the fertilizer prepared by the method of the present invention on the active ingredient content of the plant.

Plants were grown in the same manner as in the above experimental example, but using ginseng instead of lettuce, cultivated under the same conditions for one year, and the content of saponin, a major active ingredient, was measured. In addition, to measure the growth rate of ginseng, the weight of the root was also measured.

Saponin content (mg/kg) Weight (g) Example 1 128.4 1.08 Example 2 28.5 0.67 Example 3 84.8 0.84 Example 4 95.4 0.91 Example 5 67.5 0.85 Example 6 88.1 0.94

As shown in Table 2, in the case of Example 1 of the present invention, the weight was larger than that of the other examples, and the saponin content was also significantly increased. In particular, the difference from Example 2 in which MSM was not used was very high, and in the case of Example 1 of the present invention, it was confirmed that the effect of promoting growth and increasing the active ingredient was excellent. Although shown and described, the present invention is not limited to the specific embodiments described above, and various modifications can be made by those skilled in the art without departing from the gist of the present invention as claimed in the claims. Of course, these modifications should not be individually understood from the spirit or perspective of the present invention.

Claims (5)

Preparing a fertilizer containing MSM (MSM, Methylsulfonylmethane);
applying the fertilizer to the soil;
sowing ginseng in the soil where the spraying has been completed;
Mixing water and the prepared fertilizer, then adding to the soil at intervals of 10 to 30 days; and
Mixing water and the prepared fertilizer, then spraying the leaf surface at intervals of 30 to 50 days;
In a saponin content improvement method comprising a,
The step of preparing the fertilizer,
Preparing an MSM mixture by mixing 20-50 parts by weight of chitosan extracted from red crab by-products relative to 100 parts by weight of water, 5-20 parts by weight of methylsulfonylmethane, 50-80 parts by weight of seaweed extract, and 5-20 parts by weight of silicon ;
supplying ultrasonic waves to the MSM mixture, mixing and dispersing;
After the mixing and dispersing is completed, the step of loading into a storage container and storing for 20 to 50 days;
removing the solid content in the storage container after the storage is completed; and
manufacturing a fertilizer by vacuum-concentrating and freeze-drying the liquid from which the solids have been removed;
includes,
Chitosan extracted from the red crab by-product,
After washing the red crab by-product, 200 to 300 parts by weight of water and 1 to 10 parts by weight of a proteolytic enzyme relative to 100 parts by weight of the washed red crab by-product are added, and then at a temperature of 50 to 70° C. for 10 to 20 hours. a step of removing the reacted protein;
Inactivating the proteolytic enzyme by heating to 90 ~ 110 ℃ after the protein removal step;
removing calcium by immersing in 100 to 300 parts by weight of an acidic material relative to 100 parts by weight of the red crab by-product from which the protein has been removed;
Preparing chitin by immersing the red crab by-product from which the calcium has been removed in an aqueous solution of ethanol having a concentration of 3 to 5% by weight for 80 to 120 minutes;
Deacetylation by mixing 100 to 300 parts by weight of the basic solution with respect to 100 parts by weight of the prepared chitin and applying ultrasonic waves;
Washing with water after the deacetylation is completed to prepare chitosan;
adsorbing and separating impurities by adding 200 to 300 parts by weight of water and 10 to 15 parts by weight of activated carbon relative to 100 parts by weight of the prepared chitosan;
After removing the activated carbon and separating the chitosan, the separated chitosan is immersed in a solution in which 5-10 parts by weight of ascorbic acid and 5-10 parts by weight of sorbitol are mixed with 100 parts by weight of water for 5-10 days to prepare water-soluble chitosan to do; and
Concentrating the water-soluble chitosan in vacuum, then freeze-drying to prepare a water-soluble chitosan powder;
It is prepared by a method comprising
The acidic substance is at least one mixture selected from the group consisting of lemon juice, citron juice, sugar citron juice, orange juice and vinegar,
The basic solution is
separating the shells and then washing them;
calcining the washed clam shells at a temperature of 800 to 1200 °C;
pulverizing the calcined shellfish;
mixing the pulverized clam shells with 1000 to 5000 parts by weight of water based on 100 parts by weight of the clam shells, and then leaving them to stand for 2 to 10 days; and
separating the solid content of the clam shell;
It is a solution prepared by a method comprising
The ultrasonic wave has a frequency of 20 to 130 kHz and is supplied with an intensity of 300 to 800 W,
The saponin content improvement method, characterized in that the clam shell solids are fertilized in the soil.
According to claim 1,
The proteolytic enzyme is an endo-type proteolytic enzyme (Alcalase), characterized in that the saponin content improvement method.
According to claim 1,
The step of preparing the chitin is a saponin content improvement method, characterized in that the step of extracting astaxanthin from the chitin using ethanol.
3. The method of claim 2,
The seaweed extract is
preparing a seaweed mixture by mixing 5-20 parts by weight of seaweed, 20-40 parts by weight of seaweed, and 20-40 parts by weight of chlorella, relative to 100 parts by weight of kelp;
pulverizing the seaweed mixture and then drying;
loading the dried seaweed mixture into a high-pressure chamber, then supplying liquid nitrogen while maintaining 10 to 20° C. to pressurize the pressure inside the chamber to 5 to 10 atm, and maintaining it for 30 to 70 minutes;
rapidly reducing the pressure in the chamber to expand the seaweed mixture in the chamber;
mixing 100 to 200 parts by weight of water with respect to 100 parts by weight of the swollen seaweed mixture, then inoculating lactic acid bacteria and fermenting it for 10 to 50 days at a temperature of 20 to 30°C; and
preparing a seaweed extract by separating the seaweed solids that have subsided after the fermentation is completed;
Saponin content improvement method, characterized in that prepared by a method comprising a.
According to claim 1,
The adding step is,
Installing a pipe for adding at regular intervals between the sown ginseng;
Connecting the fertilizer supply for additional cost to one side of the pipe for adding;
supplying a fertilizer in which 5-10 parts by weight is mixed with respect to 100 parts by weight of water to the fertilizer supply unit for supplementation; and
Pressurizing the fertilizer mixed with the water every predetermined time to add between the planted ginseng through the pipe for adding;
includes,
The saponin content improvement method, characterized in that the tube for adding 0.1 ~ 3mm holes are formed at intervals of 10 ~ 100cm.

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