CN106561831A - Shrimp-Chinese caterpillar fungus fermented live bacteria beverage and preparation method thereof - Google Patents
Shrimp-Chinese caterpillar fungus fermented live bacteria beverage and preparation method thereof Download PDFInfo
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- CN106561831A CN106561831A CN201610913730.XA CN201610913730A CN106561831A CN 106561831 A CN106561831 A CN 106561831A CN 201610913730 A CN201610913730 A CN 201610913730A CN 106561831 A CN106561831 A CN 106561831A
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- bacteria beverage
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/06—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing non-milk proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a shrimp-Chinese caterpillar fungus fermented live bacteria beverage and a preparation method thereof. The method comprises the following steps that Chinese caterpillar funguses are inoculated to a slope culture medium for cultivation, then the Chinese caterpillar funguses are inoculated to a liquid PDA enriched culture medium for two times of scale-up cultivation, and finally the Chinese caterpillar funguses are inoculated to a liquid fermentation culture medium for deep fermentation, and the shrimp-Chinese caterpillar fungus fermented live bacteria beverage is obtained. The liquid fermentation culture medium is composed of 10-20% of fresh shrimps, 0.5-3.0% of glucose, 0.5-3.0% of saccharose, 1-5% of fresh ginger, and the balance water. The pH value is natural. The fresh shrimps serve as the major component of the liquid fermentation culture medium and are rich in nutrition, excellent nutrient substance and environment conditions are provided for the Chinese caterpillar funguses, and besides, the health care effect and taste as well as flavor of the beverage are improved.
Description
Technical field
The invention belongs to field of food, and in particular to a kind of health drink, more particularly to one seed shrimp-Cordyceps Militaris fermentation viable bacteria
Beverage and its production method.
Background technology
Shrimp, also known as " long hair is public ", " Hu Tougong ", " bent body boy " etc., it is different by source is produced, it is divided into seawater shrimps and fresh water
2 kinds of shrimp.Sea shrimp is called red shrimp, including lobster, prawn etc..But all nutritious regardless of shrimp, reasonably eating can be with benefit
Beneficial health.
Shrimp have delicious flavour, it is nutritious the characteristics of.According to analysis, 77 grams of moisture content in the fresh shrimp of every hectogram, albumen
20.6 grams of matter (being one of protein content highest food), 0.7 gram of fat, 35 milligrams of calcium, 150 milligrams of phosphorus, 0.1 milligram of iron,
The international unit of 3-Hydroxyretinol 60, also containing vitamin B1, vitamin B2, vitamin E, niacin etc..The nutritive value of dried small shrimp is more
Height, 39.3 grams of protein is contained per hectogram, and 2000 milligrams of calcium, 1005 milligrams of phosphorus, wherein 5.6 milligrams of iron, the content of calcium are moved for various
The hat of vegetable food product, is particularly suitable for the elderly and children are edible.
The meat of shrimp is soft, easy to digest, and with high nutritive value, can strengthen human body immunity and sexual function,
Tonifying kidney and strengthening yang, premature senescence resistance, antitoxin, the blood-nourishing controlling nocturnal emission with astringent drugs of logical breast, change removing toxic substances of becoming silted up, beneficial gas grow sun, remove obstruction in channels to relieve pain, resolving sputum of whetting the appetite etc..Suitably
In impotence due to deficiency of the kidney, premature ejaculation of passing out semen, alactation, arthralgia and myalgia, tetany, whole body itch, skin ulcer, in poor health and god
Jing is weak to wait patients.
At present, by Cordyceps Militaris fermented and cultured mycelium or production health drink, it is egg that the composition of used medium is most
White peptone, milk powder, analysis for soybean powder, carbohydrate and mineral element, there is not yet be formulated as primary raw material using aquatic products such as fresh shrimps
Nutrient solution.
Used as a kind of food materials, to eat raw, two is freezen protective to majority to shrimp, and three are machined to dry product.Shrimp deep processing is into other
The product such as product, mainly peeled shrimp, dried shrimps and astaxanthin class, and be processed into beverage and be particularly fermented beverage, commercially not yet
It was found that.With the class aquatic products of shrimp one as raw material, using fermentable, health food is produced into, is a kind of bold trial,
A Tiao Xin roads are opened up in deep processing for aquatic products.
Cordyceps sinensis scientific name is Cordyceps sinensis (Berk aley) Saacardo, also known as Cordyceps sinensis, winter worm
Grass, Chinese caterpillar fungus, it is a kind of extremely famous and precious rare Mycophyta Chinese medicine, with high medicine, health care and economic worth, with people
Ginseng, pilose antler and referred to as three big tonics.Wild Cordyceps sinensis has strict parasitics, and will be in special high-cold steppe
Could growth and breeding under ecological environment.Thus, yield is considerably less.The particularly severe exacerbation of ecological environment and artificial in recent years
Excessively excavation, causes yield degradation.Plus the market demand greatly, supply falls short of demand, thus results in the valency of wild cordyceps
Lattice are very expensive.Per kilogram of low tens of thousands of unit of Cordyceps sinensis market price, high wants ten thousand yuan of twenty or thirty.
Increasingly reduce to solve wild cordyceps yield, the contradiction that the market demand increasingly increases again, from last century 70
Age, many R＆D institutions of China and scientific research personnel have carried out the test of artificial cultivation of cordyceps.Up to the present, though
Have and successfully reported in laboratory test, but produce not yet have breakthrough progress in batch.A main difficult problem is：1. bacterial classification
Cultivate difficult；2. bat moth egg hatching, the raising of larva and breeding are difficult；3. artificial challenge is difficult；4. high-cold steppe ecological environment is simulated
It is difficult.
Chinese caterpillar fungus is sweet, mild-natured, enters lung kidney two warp.Beneficial lung kidney, benefit marrow, hemostasis and phlegm.With modern medicine and biology
Development, the continuous research to the medicinal function of Chinese caterpillar fungus, find Chinese caterpillar fungus have improve body immunity, antitumor, antibacterial anti-inflammatory,
Shock the effect such as anticonvulsion, anti-aging, anti-senile dementia, reducing blood lipid and hormonelike.
The main component of Cordyceps sinensis is by more than 30 kinds of inorganic elements and protein, amino acid, carbohydrate, fat, alkaloid
The organic substance institutes such as enzyme, the ucleotides such as volatile oil, cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, superoxide dismutase (SOD enzymes)
Composition.
Cordyceps sinensis main active substances have：(1) selenium element：With antitumaous effect；(2) cordycepin：With antiviral, suppression
Bacterium, anticancer, shield kidney, Dingchuan, anti-ageing various effects of waiting for a long time；(3) Cordyceps sinensis polysaccharide：With mechanism tumour, the removing toxic substances work(of liver is improved
Can, particularly there is remarkable effect to senile chronic bronchitis, cor pulmonale；(4) cordycepic acid：With reducing blood lipid, drop cranium
Pressure effect, plays the role of good to cerebral thrombus, cerebral hemorrhage；(5) SOD enzymes：Have anti-aging, it is antitumor, resist various difficult and complicated illness and
The physiological functions such as beauty；(6) containing multivitamin and mankind's indispensable element, such as：Iron, copper, zinc, molybdenum, chromium, cobalt and with urging
Dormancy, P elements that are calm, promoting nervous system metabolic function.
The mankind are nontoxic, safe healthy food by Chinese caterpillar fungus Jing toxicity tests.Long-term consumption, there is regulation body new old generation
Thank to function, strengthen the ability of resist the disease, also promote longevity, the effect of anti-aging, be a kind of preferable health beverages.
Cordyceps sinensis from natural surroundings in addition to obtaining, it is also possible to aweto strain is isolated from wild Chinese caterpillar fungus,
Again the submerged fermentation of Jing artificial eye-liquids obtains a large amount of cordyceps mycelias to aweto strain.The nutrient content of cordyceps mycelia, effectively
Composition and effect are similar to wild Chinese caterpillar fungus.
Worm winter, mycelium summer of wild cordyceps or artificial culture, by various methods, can be processed into of all kinds
Health food, nutraceutical.It is nutritious in Chinese caterpillar fungus health product, nutraceutical, containing several physiological active substances, to human body
With plurality of health care functions.
The content of the invention
In order to improve nutrition and health care effect and abundant local flavor and mouthfeel, the primary and foremost purpose of the present invention is to provide a kind of
The characteristics of production method of shrimp-Cordyceps Militaris fermentation viable bacteria beverage, the method is to prepare culture medium by raw material of fresh shrimp, inoculates worm
Careless bacterium, using liquid deep layer fermenting Chinese caterpillar fungus bacterium solution is turned out, and is subsequently processed into shrimp-Cordyceps Militaris fermentation viable bacteria beverage.
Another object of the present invention is to provide shrimp-Cordyceps Militaris fermentation viable bacteria beverage, the beverage by obtained in said method
In nutrient content, active material, enzymatic activity etc. all do not lose or be destroyed, be kept substantially ecosystem, it is ensured that product
Quality and improve the constitutional health-care effect of body immunity.
The purpose of the present invention is achieved through the following technical solutions：
The production method of one seed shrimp-Cordyceps Militaris fermentation viable bacteria beverage, comprises the following steps：
(1) slant strains culture：Under aseptic condition, Cordyceps Militaris are inoculated on slant medium, 15-22 DEG C in the dark
Lower culture a couple of days, treat that inclined-plane covers with mycelia, be used as next step Liquid Culture；
Described slant medium is PDA enriched mediums, and the formula of the culture medium is：Potato 20% (W/W), grape
1% (W/W) of sugar, peptone 1% (W/W), K2HPO40.1% (W/W), MgSO40.1% (W/W), agar 15-20% (W/W), it is remaining
Measure as water, pH value 5.5-6.5；
(2) level liquid Spawn incubation：Under aseptic condition, the bacterium of 3-5 block mung bean sizes is taken out from the inclined-plane of step (1)
Kind, in accessing level liquid culture medium, it is placed on shaking table, 15-25 DEG C of shaken cultivation 4-8d, treat to grow thick bacterium in blake bottle
Liquid or dense mycelium pellet, are used as secondary liquid culture；
Level liquid culture medium is the PDA enriched mediums for being not added with agar；
(3) secondary liquid Spawn incubation：Under aseptic condition, the level liquid nutrient solution for completing to cultivate is inoculated with into two grades of liquid
In body culture medium, inoculum concentration is 5-15% (W/W), 15-25 DEG C of shaken cultivation 4-8d, treat to be grown in blake bottle thick bacterium solution or
Dense mycelium pellet, is used as liquid fermentation and culture；
Secondary liquid culture medium is identical with level liquid culture medium；
(4) liquid fermentation and culture：Under aseptic condition, the secondary liquid nutrient solution for completing to cultivate is inoculated with into liquid fermentation training
In foster base, inoculum concentration is 5-15% (W/W), and bacterium solution is dispensed, obtained by 15-25 DEG C of oscillation and fermentation cultivation 4-8d after bacterium solution is thick
To shrimp-Cordyceps Militaris fermentation viable bacteria beverage；
The composition of described liquid fermentation medium is：Fresh shrimp 10-20% (W/W), glucose 0.5-3.0% (W/W), sugarcane
Sugared 0.5-3.0% (W/W), ginger 1-5% (W/W), balance of water, pH value natural (without the need for adjusting)；Ginger is pungent, tepor, returns
Lung taste Jing, containing pungent and fragrance ingredient, play the role of neutralize beverage it is partially cold, another ginger contains the nutrients such as flavonoids
Matter, increases the health value and flavor taste of beverage；
Preferably, described liquid fermentation medium also contains 1-3% (W/W) milk powder, and this can increase beverage nutrients
Matter and milk fragrance, mouthfeel is more preferable；
Preferably, also containing the big soya-bean milk of 5-20% (W/W), this can increase beverage nutrition to described liquid fermentation medium
Material and beany flavour, mouthfeel is more preferable；
Described fresh shrimp can be fresh water shrimp or seawater shrimps, preferred Penaeus Vannmei (Penaeus vannamei), ring
Shrimp (Penaeus Japonica), shrimp (Metapenaeus ensis), Chinese prawn (Fenneropenaeus
Chinensis), Penaeus monodon (Penaeus monodon), penaeus penicillatus (penaeus Penicillatus) or banana prawn
(Fenneropenaeus merguiensis)；Fresh shrimp will first cleaned using before, decaptitate, shell, remove enteron aisle；
Shaken cultivation of the present invention, its rotating speed is 140-180rpm.
Obtained shrimp-Chinese caterpillar fungus fermentation viable bacteria beverage appearance of the invention is in rufous, thick, there is shrimp fragrance, ginger fragrance and worm
The fragrant of the fermentation of careless bacterium, micro- sour, micro- sweet, slight bitter is in good taste.
The inventive method has carried out significant optimization to several culture mediums, achieves prominent effect：
Peptone is added to provide more rich nitrogen source, the more preferable land productivity of Cordyceps Militaris than PDA culture medium in PDA enriched mediums
With absorption, be conducive to the growth of bacterial classification.
Liquid fermentation medium main component is fresh shrimp (fresh water shrimp or seawater shrimps), and this is natural materials, nutritious, no
But good nutriment and environmental condition are provided for Cordyceps Militaris, and also improves the health-care effect and taste flavor of beverage.
Ginger is added in liquid fermentation medium, ginger is the seasoned food commonly used in daily life, containing many special
Nutriment, particularly containing pungent and fragrance ingredient.Its property is pungent, tepor, return lung taste Jing, and to the cold of insufficiency type, physique crowd is one
Plant good health food.Because Chinese caterpillar fungus bacteria beverage is in itself cold, add ginger to have cold in neutralization beverage, make Worm grass oral
Material is more suitable for various crowds and drinks, and improves its health-care efficacy.
Shrimp-Chinese caterpillar fungus fermentation viable bacteria the beverage of the present invention is the fluid nutrient medium for adopting fresh shrimp to prepare for main component, is accessed
Chinese caterpillar fungus liquid spawn, by the thick shape mycelium beverage of liquid deep layer fermenting, its advantage relative to prior art is：1、
Fresh shrimp provides native protein, various vitamins and mineral element for culture medium, output quality of making a living is higher, health-care effect more
Good, mouthfeel more preferably, the more rich beverage of local flavor provide material guarantee；2nd, thalline is living；3rd, keep ecosystem, Normal juice former
Taste, without any preservative, antistaling agent, antioxidant, essence, thickener and other additives；4th, containing abundant nutrition
Material and active ingredient, with improving body immunity and the health-care efficacy such as build up health；5th, with special shrimp fragrance and worm
It is the fragrant of careless beverage, in good taste；6th, it is safe and nontoxic.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
Embodiment 1
The production method of one seed shrimp-Cordyceps Militaris fermentation viable bacteria beverage, comprises the following steps：
(1) slant strains culture：Under aseptic condition, Cordyceps Militaris (Cordyceps sinensis (Berkaley)
Saacardo) original strain (being purchased from Guangdong Microbes Inst) is inoculated on slant medium, in the dark at 15-22 DEG C
Culture a couple of days, treat that inclined-plane covers with mycelia, be used as next step Liquid Culture.
Described slant medium is PDA enriched mediums, and the formula of the culture medium is：Potato 20% (W/W), grape
1% (W/W) of sugar, peptone 1% (W/W), K2HPO40.1% (W/W), MgSO40.1% (W/W), agar 15-20% (W/W), it is remaining
Measure as water, pH value 5.5-6.5.
(2) level liquid Spawn incubation：Under aseptic condition, the bacterium of 3-5 block mung bean sizes is taken out from the inclined-plane of step (1)
Kind, in accessing level liquid culture medium, it is placed on shaking table, 15-25 DEG C of shaken cultivation 4-8d, treat to grow thick bacterium in blake bottle
Liquid or dense mycelium pellet, are used as secondary liquid culture.
Level liquid culture medium is the PDA enriched mediums for being not added with agar；
(3) secondary liquid Spawn incubation：The level liquid nutrient solution for completing to cultivate is inoculated with secondary liquid culture medium,
Inoculum concentration is 5-15% (W/W), 15-25 DEG C of shaken cultivation 4-8d, treats to be grown in blake bottle thick bacterium solution or dense mycelia
Ball, is used as liquid fermentation and culture；
Secondary liquid culture medium is identical with level liquid culture medium；
(4) liquid fermentation and culture：The secondary liquid nutrient solution for completing to cultivate is inoculated with liquid fermentation medium, is inoculated with
Measure as 5-15% (W/W), 15-25 DEG C of oscillation and fermentation cultivation 3-8d, dispense bacterium solution after bacterium solution is thick, obtain shrimp-Cordyceps Militaris
Fermentation viable bacteria beverage；
The composition of described liquid fermentation medium is：Penaeus Vannmei 10-20% (W/W), glucose 0.5-3.0%
(W/W), sucrose 0.5-3.0% (W/W), ginger 1-5% (W/W), balance of water, pH value natural (without the need for adjusting).
Obtained shrimp-Chinese caterpillar fungus fermentation viable bacteria beverage appearance of the invention is in rufous, thick, there is shrimp fragrance, ginger fragrance and worm
The fragrant of the fermentation of careless bacterium, micro- sour, micro- sweet, slight bitter is in good taste, genuine without any food additives, bacterium be it is living,
Ecosystem, safety, containing abundant nutriment and health substance, especially polysaccharide material and various organized enzymes, they are to carrying
High body immunity and premunition, raising human body constitution are played an important role.
Embodiment 2
One seed shrimp-Cordyceps Militaris is fermented the production method of viable bacteria beverage, except also containing in the liquid fermentation medium of step (4)
Have outside 1-3% (W/W) milk powder, other raw materials and step are with embodiment 1.
The made beverage of the present embodiment increased nutriment and milk fragrance, and mouthfeel is more preferable.
Embodiment 3
One seed shrimp-Cordyceps Militaris is fermented the production method of viable bacteria beverage, except also containing in the liquid fermentation medium of step (4)
Have outside the big soya-bean milk of 5-20% (W/W), other raw materials and step are with embodiment 1.
The made beverage of the present embodiment increased nutriment and beany flavour, and mouthfeel is more preferable.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment
Limit, other any Spirit Essences without departing from the present invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (7)
1. one seed shrimp-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that comprised the following steps：
(1) slant strains culture：Under aseptic condition, Cordyceps Militaris are inoculated on slant medium, are trained at 15-22 DEG C in the dark
Foster a couple of days, treat that inclined-plane covers with mycelia, be used as next step Liquid Culture；
Described slant medium is PDA enriched mediums；
(2) level liquid Spawn incubation：Under aseptic condition, the bacterial classification of 3-5 block mung bean sizes is taken out from the inclined-plane of step (1),
In accessing level liquid culture medium, it is placed on shaking table, 15-25 DEG C of shaken cultivation 4-8d, treats to grow thick bacterium solution in blake bottle
Or dense mycelium pellet, it is used as secondary liquid culture；
Described level liquid culture medium is the PDA enriched mediums for being not added with agar；
(3) secondary liquid Spawn incubation：Under aseptic condition, the level liquid nutrient solution for completing to cultivate is inoculated with into secondary liquid training
In foster base, inoculum concentration is 5-15% (W/W), and 15-25 DEG C of shaken cultivation 4-8d is treated to grow thick bacterium solution or dense in blake bottle
Mycelium pellet, be used as liquid fermentation and culture；
Described secondary liquid culture medium is identical with level liquid culture medium；
(4) liquid fermentation and culture：Under aseptic condition, the secondary liquid nutrient solution for completing to cultivate is inoculated with into liquid fermentation medium
In, inoculum concentration is 5-15% (W/W), and 15-25 DEG C of oscillation and fermentation cultivation 4-8d dispense bacterium solution after bacterium solution is thick, obtain shrimp-
Cordyceps Militaris fermentation viable bacteria beverage；
The composition of described liquid fermentation medium is：Fresh shrimp 10-20% (W/W), glucose 0.5-3.0% (W/W), sucrose
0.5-3.0% (W/W), ginger 1-5% (W/W), balance of water, pH value nature.
2. shrimp according to claim 1-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that：The PDA adds
The formula of rich culture medium is：Potato 20% (W/W), glucose 1% (W/W), peptone 1% (W/W), K2HPO40.1% (W/
W)、MgSO40.1% (W/W), agar 15-20% (W/W), balance of water, pH value 5.5-6.5.
3. shrimp according to claim 1-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that：Described liquid
Body fermentation medium also contains 1-3% (W/W) milk powder.
4. shrimp according to claim 1-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that：Described liquid
Body fermentation medium is also containing the big soya-bean milk of 5-20% (W/W).
5. shrimp according to claim 1-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that：Described is fresh
Shrimp is fresh water shrimp or seawater shrimps.
6. shrimp according to claim 1-Cordyceps Militaris is fermented the production method of viable bacteria beverage, it is characterised in that：Described is fresh
Shrimp is Penaeus Vannmei, ring shrimp, shrimp, Chinese prawn, Penaeus monodon, penaeus penicillatus or banana prawn.
7. one seed shrimp-Chinese caterpillar fungus fermentation viable bacteria beverage, it is characterised in that：It is the method preparation by described in any one of claim 1-6
Obtain.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107190040A (en) * | 2017-06-05 | 2017-09-22 | 浙江国际海运职业技术学院 | A kind of ring shrimp anti-oxidation peptide and preparation method thereof |
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| CN104522798A (en) * | 2014-12-17 | 2015-04-22 | 中国海洋大学 | Fermented beverage with krill and preparation method of fermented beverage with krill |
| CN104522812A (en) * | 2014-12-30 | 2015-04-22 | 华南师范大学 | Cordyceps active lactic acid bacteria beverage and production method thereof |
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- 2016-10-20 CN CN201610913730.XA patent/CN106561831A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104522798A (en) * | 2014-12-17 | 2015-04-22 | 中国海洋大学 | Fermented beverage with krill and preparation method of fermented beverage with krill |
| CN104522812A (en) * | 2014-12-30 | 2015-04-22 | 华南师范大学 | Cordyceps active lactic acid bacteria beverage and production method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107190040A (en) * | 2017-06-05 | 2017-09-22 | 浙江国际海运职业技术学院 | A kind of ring shrimp anti-oxidation peptide and preparation method thereof |
| CN107190040B (en) * | 2017-06-05 | 2021-02-02 | 浙江国际海运职业技术学院 | Antioxidant peptide of penaeus japonicus and preparation method thereof |
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