KR20180063390A - Method for Preparing Mulberry Leaf Extracts - Google Patents

Abstract

A method for producing Morus alba extracts provides a materialization process in which functional materials contained in Morus alba are efficiently extracted to develop functional food materials for blood sugar control and blood circulation improvement, and there is an effect that the Morus alba extracts can be developed as health functional food materials for blood sugar control, blood circulation improvement, arteriosclerosis and the like by efficiently extracting the functional material contained in the Morus alba.

Description

Method for Preparing Mulberry Leaf Extracts [

The present invention relates to a method for preparing a leaf extract, and more particularly, to a method for producing a leaf extract, which provides a materialization process for efficiently extracting a functional material contained in a leaf, thereby developing a functional food material for controlling blood glucose and improving blood circulation.

Mulberry leaves are recorded as medicinal plants with effects such as angelic disease, edema, diabetes and decolorization. Recently, the effect of mulberry leaves to prevent and treat adult diseases such as diabetes and blood cholesterol lowering has been recognized as a material of functional foods in Korea, China, and Japan.

Mulberry contains 25 kinds of amino acids, and is known to eliminate hangover because of the high contents of alanine, aspartic acid and glutamic acid. It also contains serine and tyrosine to prevent blood circulation and senile dementia in the brain. Compared to green tea, the content of various minerals contained in mulberry leaves is 6 times that of calcium, 2 times of iron, 1.4 times of potassium It is reported to be high.

However, the products developed so far are those obtained by simply extracting the extract from the upper side and adding the alcohol to the extracted juice, or merely mixing the juice with the water and the other sweeteners, There is a problem that the demand is small due to the difference in flavor and richness.

In order to solve such problems, the present invention provides a method for preparing a leaf extract for providing a materializing process for efficiently extracting a functional material contained in a leaf, thereby developing a functional food material for controlling blood glucose and improving circulation, .

According to an aspect of the present invention,

Adding 70 liters of water to the extractor and heating to 50 캜;

A predetermined amount of dried top leaves are put into an extracting bag, put into an extractor, extracted at 50 ° C for 4 hours, and filtered through a 1 μm filter membrane;

Concentrated under reduced pressure in a vacuum concentrator at a temperature of 60 DEG C to reduce the volume by 6 times;

Sterilizing the leaf extract concentrate in a sterilizer at 80 DEG C for 30 minutes; And

And lyophilizing the extract of the extracted leaf extracts under vacuum at a predetermined condition or spray drying the extract at a predetermined temperature.

The step of freeze-drying under vacuum or spray-drying at a predetermined temperature may be carried out,

In the lyophilization step, maltodextrin content was adjusted to 8: 2 based on the solid content in the concentrate of the leaf extract, based on the solid content of the leaf extract, and the mixture was freeze-dried at -40 ° C. under a vacuum of 600 mTorr or less for 72 hours and,

In the spray drying step, the content of maltodextrin is adjusted to 8: 2 by weight based on the solid content in the concentrated liquid of the upper leaf extract, and spray dried at 240 ° C.

The method for producing the leaf extract of the present invention,

Mixing the dried and ground ginseng powder with the upper leaves at a weight ratio of 1: 1 to 3: 3, and extracting the mixture at 50 ° C for 3 hours and filtering through a 1 μm filter membrane;

Concentrated under reduced pressure in a vacuum concentrator at a temperature of 60 ° C to reduce the volume by 5 times; And

Sterilizing the extract concentrate in a sterilizer at 80 DEG C for 30 minutes.

After the sterilization step,

Lyophilizing the extract concentrate for 72 hours under a vacuum of -40 DEG C, 60 mTorr or less, and pulverizing the extract to obtain a pharmacological powder;

Drying and crushing the omiza to obtain an extender of the omija powder;

The method may further comprise the step of aggregating the mixture of the pharmacologically and omisina powders with a ring, encapsulating in a capsule for medicines or a ring, and applying a medicinal coating on the outer surface.

After the step of concentration under reduced pressure,

Mixing the extract concentrate with the reduced-pressure concentrated extract and Omija powder at a weight ratio of 1: 0.5 to 1 to produce a mixture;

Mixing the mixture with the sugar at a weight ratio of 1: 0.5 to 1, then extracting the acid with stirring for 24 hours, mixing the mixture for 1 to 10 times, and then filtering the mixture;

Sterilizing the extract concentrate in a sterilizer at 60 to 70 DEG C for 30 minutes by soaking.

According to the above-described constitution, the present invention has the effect of efficiently extracting the functional substance contained in the upper leaves and developing it as a health functional food material such as blood glucose control, blood circulation improvement, arteriosclerosis and the like.

The present invention has the effect of providing a healthy food and a health-oriented food by efficiently extracting the functional material contained in the upper leaves to realize the commercialization of the functional food.

FIG. 1 is a view illustrating a method for preparing a leaf extract according to a first embodiment of the present invention.
FIG. 2 is a view showing a method for manufacturing a leaf extract according to a second embodiment of the present invention.

Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise.

FIG. 1 is a view illustrating a method for preparing a leaf extract according to a first embodiment of the present invention.

The method for preparing a leaf extract according to the first embodiment of the present invention comprises the steps of extracting a predetermined amount of dried leaf and concentrating to produce a leaf extract concentrate (S100), sterilizing the leaf extract concentrate and freeze- And spray drying (S110) at a constant temperature.

More specifically, the step (S100) of producing a leaf extract concentrate is a step (S102) of placing 70 liters of water in an extractor and heating to 50 DEG C, and 3.5 kg of the dried top leaves are put in an extractor, (S104), a step (S104) of filtering through a 1 mu m filtration membrane, a step (S106) of concentrating the filtrate under reduced pressure at a temperature of 60 DEG C at a temperature of 60 DEG C by reducing the volume by 6 times, 0.0 > 80 C < / RTI > for 30 minutes (S108).

Specifically, the step (S110) of lyophilizing under vacuum or spray drying at a constant temperature may comprise, in the lyophilization step, the maltodextrin content to the solid content of the leaf extract in a concentration of 8 : 2, and lyophilized at -40 ° C. under a vacuum of 600 mTorr or less for 72 hours. In the spray drying step, the content of maltodextrin relative to the solid content in the leaf extract concentrate was determined on the basis of the solid content in the concentrate of the leaf extract 8: 2, and spray-dried at 240 ° C.

Mulberry leaves (Mori folium) is a dried leaf of Moraceae (Moraceae) which is a deciduous tree belonging to the upper part of the family Moris alba L., , The name of a fierce wind (to remove wind energy and clear the eyes), and to tell the effect (such as taking the powder sweating).

It is known that the leaflet suppresses the activity of α-glucosidase, an enzyme involved in the digestion of carbohydrates in the small intestine, and its hypoglycemic activity is known to be related to postprandial glucose control.

In addition to having diverse physiological functions such as antidiabetic, antioxidant, anti-inflammatory and anti-hyperlipidemic effects, the upper leaves are also effective in the treatment of various diseases such as Flavonoids, Stilbenes, Prenylflavonoids, Coumarin and Deoxynojirimycin (Deoxynoijirimycine) and the like. Also, it contains antioxidant, antitumor toxin and antidiabetic biologically active substance, and it has been widely attracted as a material of functional food.

Among these, 1-deoxynoijirimycin, which is a substance that inhibits postprandial increase of blood glucose, is analyzed as follows.

1-Deoxynojirimycin content was analyzed by HPLC.

10 μL of 0.4 M Potassium Borate Buffer (pH 8.5) and 20 μL of 10 mM 9-fluorenylmethyl chloroformate (FMOC-Cl) were added to 10 μL of the test solution and reacted at 20 ° C. for 20 minutes. Then, 10 μL of 0.1 M glycine was added to terminate the reaction. 950 μL of 0.1% acetic acid was added thereto, followed by filtration with a 0.22 μm syringe filter and analyzed by HPLC.

The concentration of 1-Deoxynojirimycin used was 200 ppm, 100 ppm, 50 ppm, 25 ppm, and 12.5 ppm.

Conditions for analyzing 1-deoxyenojirimycin are shown in the following [Table 1].

The analysis of the characteristics of the leaf extracts showed that the sugar content was 1.9 ° Brix and the sugar content of the leaf extract was 10.4 ° Brix.

The concentration of 1-Deoxynojirimycin (DNJ) was 95.39mg / 100g, and the content of 1-Deoxynojirimycin (DNJ) was 86.03mg / 100g in the spray-dried powder samples.

FIG. 2 is a view illustrating a method for manufacturing a leaf extract according to a second embodiment of the present invention.

The method for preparing the topical extract according to the second embodiment of the present invention comprises the step of injecting a mixture obtained by mixing the dried and ground ginseng powder and the topsheet at a weight ratio of 1: 1 to 3: 3 into the extractor and extracting the mixture at 50 ° C for 3 hours (S200) (S202), a step (S204) of reducing the volume by 5 times at a temperature of 60 ° C in a reduced pressure concentrator (S204), sterilizing the extract concentrate in a sterilizer for 30 minutes at 80 ° C S206).

In another embodiment, after the step of sterilizing (S206), the concentrate is lyophilized for 72 hours under vacuum at -40 DEG C, 60 mTorr or lower and then pulverized to obtain pharmacological powder; and the step of drying and pulverizing the omiza powder A step of obtaining an extender, and a step of clumping a mixture of the pharmacological powder and the omega powder into a ring, encapsulating the same in a medicinal capsule or a ring, and applying a medicinal coating on the outer surface of the medicinal powder.

Schizandrae Fructus is a deciduous broad-leaved vine, with alternate leaves, ovate or obovate, with sharp tips, sharp teeth, and slightly hairs on the back side. The flower is reddish white, bloomed in June-July, and it is migratory.

Fruit is used for food, hangs in the shape of a spike at the end of the branch, and ripens red from August to September. The fruit is called Schizandrae Fructus and Schizandrae Fructus. 200 ~ 1,600 m above sea level. It is the main province of Jirisan, Jeonbuk and Gangwon provinces. It is wild throughout the country except Chungnam and Chungbuk, and is distributed geographically in Japan, Sakhalin, Manchuria and China.

Fruits include Schizandrol, Schizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, deoxyschizandrin ) And the like. Omija has long been used as a raw material for medicinal herbs because of its medicinal properties such as astringency, nourishment, tenderness, astringency, ginger poison, thirst, astringency, Human stability due to ingestion has already been confirmed.

As another embodiment, there is provided a method for producing a mixture, comprising the steps of: (S204) a step of mixing and concentrating a concentrate under reduced pressure and an omija powder at a weight ratio of 1: 0.5 to 1; Mixing the mixture at a weight ratio, stirring the mixture for 24 hours, extracting the acid, mixing the mixture for 1 to 10 times for a certain period of time, filtering the mixture, and sterilizing the mixture by stirring at 60 to 70 캜 for 30 minutes.

Ginseng is a perennial herbaceous ginseng belonging to the ginseng group of ginseng in the food classification system. It has been known to have activities such as cholesterol lowering, blood pressure lowering, blood flow increase, antioxidant action, anti-aging action and anticancer drug.

The mixing ratio of ginseng and upturned leaves can be maximized with 1-Deoxynojirimycin (DNJ) content of 100mg / 100g or more when mixed at a one-to-one ratio and can be a functional food for hypoglycemia.

In yet another embodiment, the method for preparing a topical extract comprises the steps of immersing a mixture obtained by mixing a dried top-ground powder and an omija powder at a predetermined ratio in hot water at 50 to 60 ° C for a predetermined time to obtain a mixed extract of hot- Preparing a ginseng solution by adding purified water to ginseng extract powder; breaking the prepared ginseng solution at a temperature of 100 ° C or higher for a predetermined time; lowering the temperature of the ginseng solution to 80 to 90 ° C; Mixing the solution with the upper leaves and the omija mixed extract, heating the mixture to 80 to 90 캜, and concentrating the mixture under reduced pressure to reduce the volume by 5 times at a temperature of 60 캜 in a vacuum concentrator.

Condensation under reduced pressure, freeze-drying under a predetermined condition of vacuum or spray-drying at a constant temperature.

In the present invention, the method of adding the top leaves and the omija to the ginseng solution which has been terminated for a predetermined time and temperature is used, and it is necessary to maintain 80 to 90 ° C until sufficient decompression and concentration of the decompression- .

The embodiments of the present invention described above are not implemented only by the apparatus and / or method, but may be implemented through a program for realizing functions corresponding to the configuration of the embodiment of the present invention, a recording medium on which the program is recorded And such an embodiment can be easily implemented by those skilled in the art from the description of the embodiments described above.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It belongs to the scope of right.

Claims (6)

Adding 70 liters of water to the extractor and heating to 50 캜;
A predetermined amount of dried top leaves are put into an extracting bag, put into an extractor, extracted at 50 ° C for 4 hours, and filtered through a 1 μm filter membrane;
Concentrated under reduced pressure in a vacuum concentrator at a temperature of 60 DEG C to reduce the volume by 6 times;
Sterilizing the leaf extract concentrate in a sterilizer at 80 DEG C for 30 minutes; And
Drying the concentrated extract of the leaf extract of the present invention under freeze-drying under a predetermined condition or by spray-drying at a predetermined temperature. The method according to claim 1,
The step of freeze-drying under vacuum or spray-drying at a predetermined temperature may be carried out,
In the lyophilization step, maltodextrin content was adjusted to 8: 2 based on the solid content in the concentrate of the leaf extract, based on the solid content of the leaf extract, and the mixture was freeze-dried at -40 ° C. under a vacuum of 600 mTorr or less for 72 hours and,
Wherein the maltodextrin content is 8: 2 by weight based on the solid content in the concentrated liquid of the leaf extract, and spray drying is performed at 240 캜. The method according to claim 1,
The concentration of 1-Deoxynojirimycin (DNJ) in the lyophilized powder sample was 95.39 mg / 100 g, and the content of 1-Deoxynojirimycin (DNJ) in the spray dried powder sample was 86.03 mg / 100 g Wherein the method comprises the steps of: Mixing the dried and ground ginseng powder with the upper leaves at a weight ratio of 1: 1 to 3: 3, and extracting the mixture at 50 ° C for 3 hours and filtering through a 1 μm filter membrane;
Concentrated under reduced pressure in a vacuum concentrator at a temperature of 60 ° C to reduce the volume by 5 times; And
And sterilizing the extract concentrate in a sterilizer at 80 DEG C for 30 minutes. 5. The method of claim 4,
After the sterilization step,
Lyophilizing the extract concentrate for 72 hours under a vacuum of -40 DEG C, 60 mTorr or less, and pulverizing the extract to obtain a pharmacological powder;
Drying and crushing the omiza to obtain an extender of the omija powder;
Further comprising the step of aggregating the mixture of the pharmacological powder and the omija powder into a ring, encapsulating the mixture in a capsule for medicines or a ring, and applying a medicinal coating on the outer surface of the capsule. 5. The method of claim 4,
After the step of concentration under reduced pressure,
Mixing the extract concentrate with the reduced-pressure concentrated extract and Omija powder at a weight ratio of 1: 0.5 to 1 to produce a mixture;
Mixing the mixture with the sugar at a weight ratio of 1: 0.5 to 1, then extracting the acid with stirring for 24 hours, mixing the mixture for 1 to 10 times, and then filtering the mixture;
Further comprising the step of sterilizing the extract concentrate in a sterilizer at 60 to 70 DEG C for 30 minutes with stirring.

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