KR20180027375A - Compositions for Moisturizing Skin Containing Vegetable Vernix Ingredients - Google Patents

Abstract

The present invention relates to a make-up composition for skin moisturizing including a plant vernix caseosa component and, more specifically, to a make-up composition for skin moisturizing including a natural moisturizing facture, a horny layer similar component, and vegetable oil as effective components. According to the present invention, the make-up composition is effective for a skin moisturizing increase through skin barrier enhancement and is effective especially for infants.

Description

[0001] The present invention relates to a composition for moisturizing a skin,

The present invention relates to a cosmetic composition for moisturizing, which comprises a botanical ingredient as an active ingredient, and more particularly to a cosmetic composition for skin moisturizing which contains a natural moisturizing factor mixture, a stratum corneum-like component and a vegetable oil as an active ingredient.

Skin is the largest organ of the body. It is always in direct contact with the external environment. It protects the body from various stimuli or dry environment. It protects the inside of the body from moisture loss and protects it from harmful ultraviolet rays from the sun. And it plays a very important role in controlling the body temperature.

In normal adult skin, the skin is fully developed and can sufficiently serve as a protective film against external harmful factors. However, since the main accessory organs such as the skin and the sweat glands are still immature in the baby, the transdermal water loss is high, It is easy to damage the skin due to it becoming resistant and resistant.

Also, a newborn baby has the same skin structure as an adult but is less functional. Skin protection is a basic requirement and important for babies because it is about the thickness of adult skin after 12 months. Such baby skin is thin, soft, sensitive to external stimuli and bacterial infections, and easy to penetrate. As a result, perspiration or germs remaining on the skin may penetrate into the skin again, which can cause skin irritation and skin trouble. In other words, baby skin is not only fragile, delicate, but sensitive, so it is prone to skin troubles even with a small external stimulus. Therefore, it is also important to select and use stimulant-free ingredients while enhancing skin immunity.

Vernix Caseosa is a viscous white creamy skin cream covering the whole body of a newborn during childbirth. The fetus is a remnant of the fetal epidermis and is formed at the end of pregnancy. Despite the less developed skin structure of the fetus in the uterus before birth, the reason for protecting the skin in the amniotic environment is that the embryo protects the skin of the fetus, promotes the maturation of the stratum corneum, Because. In addition, it has been reported that it plays a role in regulating body temperature of fetus even after birth, antibacterial, washing, hydration, and maturation of the skin.

As a result of application to the experimental model in which the skin barrier was collapsed, the skin barrier recovery effect was superior to that of the control group such as appearance of corneocyte, reduction of epidermal thickness and scarring after 6 hours without erythema (Non-Patent Document 1 . Reference). This suggests that Taeji can be used for cosmetics and medicines as well as promoting the formation of stratum corneum in the skin under 12 months, normalizing epidermal thickness, strengthening skin barrier.

Although there is an example applied to cosmetics using existing precious ingredients, safety and quality of raw materials are difficult to obtain because of using animal originated ingredients. In addition, in the case of a cosmetic composition prepared from a sea-like component, this is merely about an underwater type composition, and does not show a sufficient moisturizing effect.

1. Korean Registered Patent No. 10-1495679

1. Oudshoorn, M.H.M. Development of a murine model to evaluate the effect of vernix caseosa on skin barrier recovery. Amsterdam: Experimental Dermatology, volume 18, issue 2, pp. 178 - 184 (2009)

Accordingly, in order to provide a cosmetic composition having a more stable and moisturizing effect, the present inventors selected plant-derived ingredients similar to the moisturizing lip balm and prepared the liposomal formulation in an optimum ratio. The inventor of the present invention has confirmed that the cosmetic composition containing the wild-type component of the present invention has sufficient moisturizing power and skin barrier strengthening effect, and completed the present invention.

Accordingly, a problem to be solved by the present invention is to provide a cosmetic composition for skin moisturizing which comprises a natural moisturizing factor mixture, a stratum corneum-like component and a vegetable oil as an active ingredient.

Another object of the present invention is to provide a method for producing a cosmetic composition for skin moisturizing which comprises a natural moisturizing factor mixture, a stratum corneum-like component and a vegetable oil as an active ingredient.

Another object of the present invention is to provide a method for enhancing skin moisturizing power by using a cosmetic composition for skin moisturizing comprising the natural moisturizing factor mixture, stratum corneum-like component and vegetable oil as an active ingredient of the present invention.

In order to solve the above-mentioned problems, the present invention provides a cosmetic composition for skin moisturizing which comprises a natural moisturizing factor mixture, a stratum corneum-like component and a vegetable oil as an active ingredient.

The present invention also provides a method for preparing a cosmetic composition for moisturizing skin, which comprises a natural moisturizing factor mixture, a stratum corneum-like component and a vegetable oil as an active ingredient.

The present invention also provides a method for enhancing skin moisturizing power or skin elasticity using the cosmetic composition.

Hereinafter, the present invention will be described more specifically.

The present invention relates to a liposome-type composition comprising a plant-derived component and having a lipid function, wherein the lipid composition comprises a natural moisturizing factor mixture and a stratum corneum-like component, and the oil phase comprises a vegetable oil, .

As used herein, the term " plant and physeal component " refers to a component obtained by replacing a natural component of natural origin composed of 80% water, 10% protein and 10% lipid component with a plant component. Specifically, ≪ / RTI > and a vegetable oil.

The weight% of the natural moisturizing factor mixture, stratum corneum-like component and vegetable oil comprises 5 to 15% by weight of a natural moisturizing factor mixture, 0.01 to 3% by weight of a stratum corneum-like component and 0.01 to 10% by weight of a vegetable oil, based on the total weight of the cosmetic composition . More preferably 7 to 13% by weight of the natural moisturizing factor mixture, 0.1 to 2% by weight of the stratum corneum-like component and 0.1 to 5% by weight of the vegetable oil.

The natural moisturizing factor mixture refers to a water-soluble component present in the stratum corneum and serves to capture moisture from the stratum corneum. The natural moisturizing factor mixture may be sodium pyrolidonecarboxylate, sodium lactate, urea or amino acid.

The cosmetic composition of the present invention contains a natural moisturizing factor mixture as an active ingredient. Preferably, the natural moisturizing factor mixture may comprise at least one member selected from the group consisting of sodium pyrrolidone carboxylate, sodium lactate, urea and amino acid.

The amino acid may be one or more selected from the group consisting of serine, glycine, arginine, glutamic acid, tyrosine and alanine, but is not limited thereto. no.

The specific content of the components constituting the natural moisturizing factor mixture is 1 to 50% by weight of sodium pyrrolidonecarboxylate, 1 to 50% by weight of sodium lactate, 1 to 20% by weight of urea, 1 to 20% by weight of amino acid, Preferably from 3 to 40% by weight of sodium pyrrolidonecarboxylate, from 1 to 40% by weight of sodium lactate, from 1 to 15% by weight of urea, from 10 to 30% by weight of amino acids, 90% by weight of purified water and 0.01 to 40% by weight of purified water, more preferably 5 to 35% by weight of sodium pyrrolidonecarboxylate, 1 to 35% by weight of sodium lactate, 1 to 12% by weight of urea, To 85% by weight of water and 0.1 to 35% by weight of purified water.

The content of the natural moisturizing factor mixture is preferably 5 to 15% by weight based on the total weight of the cosmetic composition. When the content of the natural moisturizing factor mixture is less than 5% by weight, the moisturizing effect is insufficient. When the content of the natural moisturizing factor mixture exceeds 15% by weight, unpleasant raw material odor peculiar to the natural moisturizing factor mixture is generated and the formation of the structure is inhibited. And thus adversely affect the stability of the device. Most preferably, the natural moisturizing factor mixture may comprise 7 to 13% by weight.

The normal stratum corneum has several adaptive mechanisms for maintaining and regenerating the skin in a structurally and functionally intact condition, but normal development of the baby skin, which is not fully developed, such as increased transdermal water loss and increased skin pH, is difficult . In the present invention, citric acid, lactic acid, and the like, which can act as an acidic membrane of the prey, can be contained so that the stratum corneum can maintain a normal pH range and function as a physiological barrier.

The stratum corneum acts as a primary skin barrier that protects the skin from the skin and external stimuli. However, infants less than 12 months of age are not fully functioning because the structure of the skin is not fully formed. Therefore, the stratum corneum-like component contained in the cosmetic composition of the present invention includes all the components that function as the stratum corneum layer attached to the stratum corneum surface.

Specifically, the stratum corneum-like component of the present invention can be composed of bentonite, olive oil decyl ester, citric acid, lactic acid and squalene, and additionally from the group consisting of glycerin, hydrated silica, chlorella bulguris and sea salt And may include any one or more selected.

The Chlorella bulgaris is a vegetable component of seaweed, and the chlorella bulgaris in the present invention can be preferably chlorella bulgaris extract. Chlorella vulgaris extract is rich in microalgae booster amino acids and is effective in constituting stratum corneum-like components.

When the cosmetic composition containing the stratum corneum component is applied to infants whose keratin is not sufficiently thickened, it exhibits an effect similar to that formed with thick keratin so as to enhance skin barrier and enhance moisturizing power.

The vegetable oils contained in the cosmetic composition of the present invention may be selected from the group consisting of olive oil, blackcurrant seed oil, sunflower seed oil undeclared oil, balloon vine oil, plant derived tocopherol, sunflower seed oil, rosemary leaf oil, rice bran oil, phytosqualane, Grape seed oil, jojoba oil, and camomile seed oil, and may be used without any particular limitation. Preferably, the oil is selected from the group consisting of blackcurrant seed oil, sunflower seed oil unsubordifed oil, inflorescence oil, plant-derived tocopherol, sunflower seed Oil, and rosemary leaf oil.

The vegetable oil of the present invention may be contained in an amount of 0.01 to 10% by weight, preferably 0.1 to 8% by weight, more preferably 1 to 5% by weight based on the total weight of the vegetable oil. have.

The vegetable oil is contained in the vegetable liposomal oil phase portion, which additionally imparts a sedative effect on stimulation.

The term 'plant vegetable liposome' as used in the present invention means a liposome containing the plant vegetable component of the present invention. Preferably means the liposome made through Example 1.

Black current ( Ribes nigrum ) is a deciduous shrub of rosewood bumblebees and is also called 'Western currant bush.' It is native to Europe and Central Asia. It grows well in the shade, is strong against the cold, and has a height of 1 ~ 2 m. The blackcurrant oil obtained by the carbon dioxide extraction method contains Linoleic Acid, which plays an important role in ceramide-1 production, and γ-Linolenic Acid, which promotes the production of anti-inflammatory prostaglandin. It is a component that helps strengthen skin barrier.

Unsaponifiable material is a fraction of sunflower seed that has not been saponified during the purification process of sunflower seed oil fatty acid. Sunflower seed oil insolubles contain nutrients to the skin, including ingredients like phytosterol, plant-derived tocopherol, and squalane.

Inflatable vine is a perennial herbaceous perennial plant belonging to the perennial plant of the dicotyledonous plant. It mainly contains phytosterol, flavonoid, saponin, tannin, etc., and is used as the phospholipase A2 and the cyclooxygenase- ), It has the itching, tingling, inflammation relieving effect and protects the skin which is sensitive to the stimulus. It is a mild botanical ingredient that can replace hydrocortisone, one of the anti-inflammatory steroid ingredients.

In addition, phytosqualene, grape seed oil, jojoba oil, or camphor seed oil may be included in the vegetable oil, but are not limited thereto.

The cosmetic composition of the present invention may further comprise a lipid component of a skin barrier. More specifically, the lipid component of the skin barrier may be at least one selected from the group consisting of triglyceride, ceramide, and phytosterol, and may be contained in an amount of 0.1 to 2% by weight.

Triglyceride is an ester bond of three molecules of fatty acid to glycerol and is the most widely distributed fatty acid derivative found in nature. Triglycerides are also one of the skin-like ingredients. The triglyceride used in the present invention may more preferably be caprylic / capric triglyceride.

Ceramides have hydrophilic and lipophilic groups at the same time, so they inhibit water evaporation in the skin. In addition, it functions as a skin barrier that prevents the penetration of foreign substances and plays an important role in the control of inflammation and wound healing.

Phytosterol refers to plant sterols that are structurally similar to normal cholesterol. Phytosterol has the function of restoring and maintaining the skin barrier, and is safe because it is a plant ingredient.

The cosmetic composition of the present invention is characterized by enhancing the skin moisturizing effect by strengthening the skin barrier. In one concrete example, when the plant lipase and the co-liposome were respectively applied to the skin, it was confirmed that the water increase rate and the transdermal water loss were small in the group to which the plant lipase was applied. In addition, the expression level of the moisturizing evaluation index was measured, and it was confirmed that the expression of fila green and aquaporin-3, proteins constituting the cornified envelope, was increased. In other words, it was confirmed that the vegetative liposomes are effective in enhancing the moisture retention by strengthening the keratin membrane and enhancing the moisture retention.

The cosmetic composition of the present invention may be in liposome form. The water phase portion of the liposome contains a natural moisturizing factor mixture and an acidic component, and the skin barrier lipid component and the vegetable oil component are contained in the phospholipid layer of the liposome.

Capsules that can be captured within the phospholipid layer of the liposome may include ceramides, inflatable leaf oil, blackcurrant seed oil, sunflower seed oil, or phytosterol.

The liposome-type composition of the present invention can be easily absorbed through transdermal application, and can exhibit a more effective moisturizing effect.

In addition, the cosmetic composition of the present invention may contain components commonly used in cosmetic compositions, and examples thereof include surfactants, pH adjusting agents, perfumes, preservatives, thickeners, sequestering agents, emulsifiers, solubilizers, humectants, , Ultraviolet light scattering agents, vitamins, plant extracts, skin astringents, anti-inflammatory agents, cell activators, vasodilators, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, stabilizers, solubilizers, An antioxidant, an antioxidant, an antioxidant, a blood circulation enhancer, and a skin agitation agent, and a carrier, and the formulations and the additive components are not limited to the above contents. In addition, the components can be introduced in amounts commonly used in the dermatology field.

Examples of the surfactant include, but are not limited to, anionic surfactants such as higher fatty acid soaps, alkyl sulfate ester salts, polyoxyethylene alkyl ether sulfate, alkyl ether phosphate ester salts, N-acyl amino acid salts and acyl N- Surfactants; Cationic surfactants such as alkyltrimethylammonium chloride and dialkyldimethylammonium chloride; Amphoteric surfactants such as alkyldimethylaminoacetic acid betaine, alkylamido dimethylaminoacetic acid betaine and 2-alkyl-N-carboxy-N-hydroxyimidazolinium betaine; And nonionic surfactants such as polyoxyethylene type, polyhydric alcohol ester type, and ethylene oxide / propylene oxide block copolymer. Further, without limitation, surfactants belonging to polymeric surfactants or natural surfactants can also be used.

The pH adjusting agent may be selected from the group consisting of triethanolamine, tartaric acid, sodium silicate, sodium hydroxide, sodium citrate, sodium carbonate, sodium borate borate, sebacic acid, potassium hydroxide, phosphoric acid, malic acid, magnesium hydroxide, magnesium carbonate, lactobionate, Lactobionic acid, lactic acid, glycolic acid, gamma linolenic acid, fumaric acid, diethanolamine, citric acid, borate, 1 is selected from the group consisting of benzoic acid, azelaic acid, ascorbic acid, aminomethyl propanol, and acetic acid. Sangil be, but is not limited to this.

Examples of the fragrance include, but are not limited to, vegetable fragrances separated from flowers, leaves, branches, rinds, etc.; Animal spices such as musk and shibt; Hydrocarbons such as monoterpene; Alcohols such as aliphatic alcohols and aromatic alcohols; Aldehydes such as terpene aldehyde and aromatic aldehyde; Ketones such as alicyclic ketones; Esters such as terpene-based esters; Lactones; Phenols; Oxides; Nitrogen-containing compounds; Acetals and the like. Natural and synthetic fragrances other than those exemplified above may also be used without particular limitation.

As the preservative, ethyl p-oxybenzoate, salicylic acid, sorbic acid and the like can be mentioned.

Examples of the incrementing agent include hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxyvinylpolymer, alkyl-modified carboxyvinylpolymer, polyacrylamide, Sodium alginate, propylene glycol alginate, agar, sodium polyacrylate, succinoglucan, dextran, mannan, pectin, ), Gellan gum, carrageenan, hyaluronic acid, polyvinyl alcohol, bentonite, aluminum silicate, magnesium silicate, laponite Laponite, hectorite, and silicic acid anhydride.

Examples of the metal ion sequestering agent include edetate salts such as disodium ethylenediaminetetraacetate, edetate, and sodium edetate.

Examples of the emulsifier include phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, palmitic acid, hydrogenated phophatidylcholine (derived from soybean), phosphatidylserine, phosphatidyl At least one phospholipid selected from the group consisting of phosphatidylglycerol, phosphatidylinositol, and the like can be used.

Other emulsifiers may be used, such as Cetearylolivate, Sobitanolivate (from Olive).

As the emulsifier, two or more emulsifiers may be used together. For example, phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, and hydrogenated phosphatidylcholine (derived from soybean) may be used as the first emulsifier and second emulsifier, respectively. In this case, the content of the first emulsifier may be 1 to 5 wt%, and the content of the second emulsifier may be 0.5 to 2 wt%.

Further, in addition to the first emulsifier and the second emulsifier, a composition of cetearylolivate and sorbitanolivate (oligo-derived) may be further used as the third emulsifier. In this case, 5% by weight, the second emulsifier component is 0.5 to 2% by weight, and the third emulsifier component is 0.5 to 2% by weight.

Examples of the solubilizing agent include propylene glycol fatty acid esters such as glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, and propylene glycol monostearate; glycerin, diglycerin, ethylene glycol, propylene glycol, And polyhydric alcohols such as phenol and phenol.

Examples of the moisturizing agent include hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate, polyoxyethylene methyl glucoside, polyoxypropylene methyl glucoside, lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, And the like.

As the solvent, distilled water, butylene glycol, propylene glycol, glycerin, and ethanol may be used, and distilled water, glycerin, and more preferably distilled water may be used. The content of the solvent is the residual amount of the content of the emulsifier, the collectable material and the preservative.

In addition to the above, it is also possible to use a dye, a pigment, an antioxidant, a stabilizer, a solubilizer, a refreshing agent, a deodorant, an ultraviolet absorber, an ultraviolet scattering agent, a vitamins, a plant extract, a skin astringent, And carriers and the like can be included in the cosmetic composition as long as they are commonly used in the art.

The cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art. In one embodiment, the cosmetic composition may be any one selected from the group consisting of a high viscosity emulsifier type, a low viscosity emulsifier type and a solubilized formulation, And may include a cosmetic composition composition component that is commonly used in the art to which the present invention belongs, depending on the formulation to be produced.

For example, as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays But is not limited thereto. More specifically, the present invention relates to a cosmetic lotion composition comprising: (A) at least one cosmetic composition selected from the group consisting of a lotion, a nutritional lotion, a nutritive cream, a massage cream, an essence, an eye cream, a sun lotion, a sunscreen, a makeup base, a bib cream, a cleansing cream, a cleansing foam, a cleansing water, Balm type product, spray or powder.

Preferably, the cosmetic composition of the present invention is used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, , A cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, and a body cleanser.

In the cosmetic composition of the present invention, when the formulation is a paste, a cream or a gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Zinc oxide and the like may be used.

In the cosmetic composition of the present invention, when the formulation is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Tartaric acid esters, fatty acid esters of glycerol aliphatic esters, polyethylene glycols or sorbitan can be used.

 In the cosmetic composition of the present invention, when the formulation is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester Suspending agents, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

In the cosmetic composition of the present invention, when the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

In the cosmetic composition of the present invention, when the formulation is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

In the cosmetic composition of the present invention, if it is a cleansing formulation containing a surfactant or a cleansing formulation without a surfactant or a soap, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-free cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

In the production method of the present invention, it can be illustrated in Fig. 1 of the present invention.

In a specific embodiment, the present inventors prepared each water or oil mixture by putting the exemplified oil component (B) or water component (A) into the dissolution tank and warming them to an appropriate temperature and dissolving them. After preparing each of the oil-based and water-based mixtures as described above, the oil-based mixture is added to the water-based mixture in the step (d) in the production method of the present invention to prepare a first emulsified composition. Preferably, a homogenizer is used in the emulsification.

Further, in the method of manufacturing a cosmetic composition according to the present invention, the additional ingredients are added to the first emulsified composition in step (e), and then the mixture is emulsified by using a microfluidizer (M / F) The emulsion is elevated so that the particles in the composition are uniformly and finely nanosized and stabilized to produce a plant-derived liposome. The pressure for the second emulsification is preferably 500 to 2,000 bar, more preferably 1,000 bar.

The nano-emulsified cosmetic composition produced by such a manufacturing method is composed of fine and uniform nanoparticles having an average particle size of preferably about 30 to 500 nm. A more preferred average particle size is comprised between 30 and 300 nanometers.

The moisturizing cosmetic composition comprising the botanical ingredient of the present invention as an active ingredient can be safely used on the skin of infants and young children by using a vegetable ingredient similar to natural prey and is capable of safely using both the aqueous component and the oily ingredient in the liposome, It is possible to provide excellent skin moisturizing effect and skin barrier strengthening effect.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 illustrates a method of making a composition comprising a plant-based ingredient.
Figure 2 is a schematic representation of a method of making a liposome comprising a pseudo-premix component.
FIG. 3 is a graph showing cytotoxicity of a liposome-type composition containing a plant-based ingredient.
FIG. 4 is a graph showing the moisture resistance of a liposome type composition containing a plant-based ingredient.
FIG. 5 is a graph showing the expression levels of Filaggrin (a) and Involucrin (b), which are indicators of moisture resistance, through RT-PCR.
FIG. 6 is a graph showing the expression levels of Aquaporin-3 (a) and HAS-1 (b), which are indicators of moisture resistance, through RT-PCR.
FIG. 7 is a graph showing the expression levels of HAS-2 (a) and HAS-3 (b), which are indicators of the moisture resistance, through RT-PCR.
FIG. 8 is a graph showing the amount of percutaneous water loss according to the stratum corneum-like component content.
9 is a graph showing the pH buffering effect of Example 1 and Comparative Example 1. Fig.
10 is a skin irritation relaxation graph according to vegetable oil content.
11 is a graph showing the amount of transdermal water loss in Formulation Example 1 and a control group measured in adults.
12 is a graph showing the amount of transdermal water loss in Formulation Example 2 and a control group measured in adults.
13 is a graph showing the skin moisture content of Formulation Example 2 and control group in infants.
14 is a graph showing changes in skin moisture content of Formulation Example 2 and a control group in infants.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the examples and comparative examples are provided only for the understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

Production of plant-derived liposomes

The composition of natural native fish is composed of 80% water, 10% protein, 10% lipid component, which is structurally similar to its ceramide, a lactic acid functionally similar to cholesterol, a natural moisturizing factor mixture, vegetable oil and squalene And so on.

The production method of Example 1 (plant-derived liposome) and the control (co-liposome) and the production method of the similar lipoic acid liposome are specifically shown in FIG. 1 and FIG. The differences in the components are shown in Table 1 below. (B) (specifically, a natural moisturizing factor mixture, glycerin, lactic acid, an emulsifying agent, and a preservative) are put into a dissolution tank and heated at 70 to 80 ° C to dissolve them. Was prepared. The composition of the natural moisturizing factor mixture in the water component is as shown in Table 2 below.

(Unit: wt%) shape ingredient Example 1
(Plant-derived liposome) Comparative Example 1
(Ball liposome) Comparative Example 2
(Pseudotyped liposomes) Award (B) Purified water To 100 To 100 To 100 Natural moisturizing factor mixture 10 - - Stratum-like components (bentonite, olive oil decyl ester, citric acid, lactic acid and squalene) 2 - - glycerin 25 25 - Hydrogenated lecithin 4 4 2 Remuneration (A) Caprylic / capric triglyceride 15 15 - Triglyceride - - 4 Cholesterol ester - - 10 Ceramide - - 5 Squalene - - One Addition (C) Free fatty acid - - 2 Ceramide 2 - 2 Phytosterol One - - Vegetable oils (blackcurrant seed oil, sunflower seed oil unsubstantiated, balloon vine oil) 2 - -

Raw material name Content (% by weight) Sodium pyrrolidone carboxylate 12.0 Sodium lactate 5.0 Urea 7.0 Serine 18.2 Glycine 9.1 Arginine 3.2 Glutamic acid 2.3 Tyrosine 0.5 Alanine 6.6 Purified water To 100

The oil mixture was added to the water mixture thus prepared to prepare a first emulsion composition. Thereafter, an additional component (C, specifically ceramide, vegetable oil, and phytosterol) was added to the primary emulsion composition, and the secondary emulsion composition was applied to the primary emulsion composition using a microfluidizer (M / F) The emulsion was applied to make the particles uniform and fine nano-sized. This kept the plant-derived liposomes stable.

[Experimental Example 1]

Cytotoxicity of vegetative liposomes

HaCaT (Human Skin Keratinocyte) cell line was cultured in the presence of 5% CO ₂ , 10% fetal bovine serum (FBS, Lonza), 50 μg / mL of streptomycin And cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with Sigma for 24 hours. After the culture, the medium was removed, and the plant-derived liposome prepared in Example 1 was diluted in a medium to a concentration of 0 to 2%, and the diluted solution was treated and cultured for 24 hours. After the medium was removed, a solution of 3 mg / ml of MTT (3- [4,5-dimethylthiazol-2yl] -2,5-diphenyltetrazolium bromide, Sigma, USA) was added and further cultured for 4 hours. Then, the supernatant was removed, and 150 μl of dimethylsulfoxide was added, followed by mixing for 30 minutes. The resulting formazan was dissolved and the absorbance was measured at 540 nm using a multi-microplate reader (Molecular device Spectra max 190). Cell viability was calculated according to the following equation.

[Equation]

Cell survival rate (%) = absorbance of the sample-added group / absorbance of the control group X 100

As a result, as shown in FIG. 3, it was confirmed that there was no cytotoxicity up to a concentration of 1-2% of plant-derived liposome.

[Experimental Example 2]

To check the moisture of plant native liposomes

(1) Moisture resistance measurement immediately after application

The skin moisturizing power was measured using a Corneometer CM825 (Courage-Khazaka eletronic GmbH, Germany). Measurements were taken before, immediately after, and 120 minutes after use of the test product. Corneometer probe was contacted to the skin 3 times through the sensor, and the average value was used as the evaluation value of skin moisture. The unit is expressed as a unit constant, A.U, the amount of skin moisturizing is proportional to the measured value.

As a result, as shown in FIG. 4, the moisturizing growth rate of 2.99% in the uncoated group, the moisture increase rate of 95.24% in the co-liposome (Comparative Example 1), and the moisturizing growth rate of 134.8% in the similar enchanting composition (Comparative Example 2) , And vegetative liposomes (Example 1) showed a moisture increase rate of 178.53. That is, the plant-derived liposome (Example 1) was compared with the co-liposome (Comparative Example 1) and the similar anticancer composition (Comparative Example 2) compared to the co-liposome (Comparative Example 1) Example 1) showed excellent moisture resistance after 120 minutes of application.

(2) Measurement of expression amount of moisture index

In order to confirm the moisturizing effect of plant-derived liposomes, the following parameters were determined for the production of moisturizing factors: Filaggrin, Involucrin, Aquaporin-3, HAS-1, HAS-2 and HAS-3 I examined the effect.

The cells used in the experiment were HaCaT (Human Skin Keratinocyte cell line). After treatment with 1%, 2% or 4% of the control group or Example 1, respectively, the expression level of the moisture- Were measured. The HaCaT cell line was cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with 5% CO ₂ , 10% fetal bovine serum (FBS, Lonza) and 50 μg / mL streptomycin Respectively. For RNA analysis, total RNA in cells was extracted from cell culture using Trizol reagent (Invitrogen, USA). The purity and integrity of the RNA was determined by measuring the A260 nm / A280 nm ratio, and the RNA yield was measured by absorbance at 260 nm.

For cDNA synthesis, 3 ㎍ of total RNA was added at 25 ° C at 25 ° C with addition of Oligo dT 15 (500 ng / ㎕) primer, dNTP (10 mM), RTase inhibitor (40 U / ㎕) and Powerscript Ⅱ RTase (Clontech, USA) CDNA was synthesized by primer annealing at 42 ° C for 60 min and RTase denaturation was performed at 95 ° C for 5 min. PCR was performed using 3 cDNA of cDNA, 10 ta taq (SEQ ID NO: 2) to amplify Filaggrin, Involucrin, Aquaporin-3, HAS-1, HAS-2, HAS-3 and GAPDH from cDNA. 5 μl of polymerase buffer, 2 μl of 10 mM dNTP, 2 μl of each 10 pmol primer, and 0.5 μl of taq polymerase were mixed and distilled water was added to adjust to 50 μl and amplified. The fryer sequences are shown in Table 3. The PCR product was electrophoresed on 1% agarose gel and analyzed with an image analyzer (UGEN, U: Genius). The density of each band was measured using a density measurement program (Gene Tools from Syngene) Respectively.

Gene Primer GAPDH F: 5'-AAC GAA TTT GGT CGA ACA GC -3 ' R: 5'-TGA GGA GGG ATT CAG TG -3 ' HAS-1 F: 5'-GAA TCC TTC GAC TGG GTC CTC AG -3 ' R: 5'-GAC ACG ACG CAG ACA AGA TG -3 ' HAS-2 F: 5'-GCA AAC CTA TTT GAC CAT CG -3 ' R: 5'-GCC ACT TCT GTC TAC TCA GTG -3 ' HAS-3 F: 5'-GAG GAC TGG TAC CAT CAG AA-3 ' R: 5'-CGA TGG TAG TTG TTT AGA CCG -3 ' Filaggrin F: 5'-GTT ACA ATT CCA ATC CTG TTG TTT TC -3 ' R: 5'-CGT TGC ATA ATA CCT TGG ATG ATC -3 ' Involucrin F: 5'-GTG GGG GAG AGA GGG AAT TA -3 ' R: 5'- CTC ACC TGA GGT TGG GAT TG -3 ' Aquaporin-3 F: 5'-TCA TTA TGC TGA GGA TTT -3 ' R: 5'-GGC CTC CCA TCT CCT TCA -3 '

As a result, the results of Figs. 5 to 7 were derived. In both experimental groups, the expression of HAS-1, HAS-2, HAS-3 and inbolucrin was not affected, while the expression of pilgarin and aquaporin-3 was dose dependent in Example 1 , But the expression level of the control group was not changed. These results suggest that there is no effect of moisturizing by increasing the synthesis of hyaluronic acid which affects the moisturizing effect but it is effective in increasing the expression of fila green and aquaporin-3, proteins forming the cornified envelope . In Vitro Results Plant lipase was found to be effective in moisturizing by strengthening keratin membranes and enhancing water retention.

[Experimental Example 3]

Determination of moisture content according to the content of natural moisturizing factor mixture

In order to confirm the effect of improving the moisturizing power of the plant-derived liposome according to the content of the natural moisturizing factor mixture, plant-derived liposomes were prepared at the composition ratios shown in Table 4 below. The moisture resistance was measured by the method of Experimental Example 2-1. The results are summarized in Table 5 below.

The skin moisture content was measured before and immediately after use to confirm the persistence of skin moisturization by using each test product. Changes in skin moisture content were determined by statistical methods.

  (Unit: wt%) shape ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Awards
(A) Purified water To 100 To 100 To 100 To 100 To 100 To 100 Natural moisturizing factor mixture 10 0 3 5 15 20 Stratum-like components (bentonite, olive oil decyl ester, citric acid, lactic acid and squalene) 2 2 2 2 2 2 glycerin 25 25 25 25 25 25 Hydrogenated lecithin 4 4 4 4 4 4 Paid
(B) Caprylic / capric triglyceride 15 15 15 15 15 15 adding
(C) Ceramide 2 2 2 2 2 2 Phytosterol One One One One One One Vegetable oils (blackcurrant seed oil, sunflower seed oil unsubstantiated, balloon vine oil) 2 2 2 2 2 2

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Moisture improvement rate (%) 137% 88% 107% 120% 138% 133%

The results of repeated measures ANOVA (Bonferroni correction) and Independent samples t-test were used to determine the skin moisture content, which was significantly increased immediately after use (p <0.05) , And there was a significant difference immediately after use compared to the non - implanted area. In addition, when the natural moisturizing factor mixture was less than 5 wt% (Examples 2 to 3), no significant difference was observed in moisture resistance, and when 5 to 15 wt% (Examples 1 and 4 to 5) I could.

However, when it is more than 15% by weight, there is a moisturizing effect, but it is confirmed that there is a problem of deteriorating product stability when it is applied to a cosmetic product by interfering with the formation of unpleasant raw materials and formation of a structure.

[Experimental Example 4]

Strengthening of skin barrier according to content of stratum corneum

(1) Percutaneous water loss measurement for adults

In order to confirm the skin barrier strengthening effect of the plant-based liposome according to the content of the stratum corneum-like component, the plant-derived liposome was prepared at the composition ratios shown in Table 6 below.

Experiments were conducted on the effects of skin barrier improvement of Examples 1, 7, 8 and Comparative Examples 1 and 2 on the amount of percutaneous water loss in adults. The results are summarized in Table 7 below. (However, in the case of Example 9, the formulations were united and unstable in the prescription containing 3% or more of the stratum corneum component, so that it was excluded from the experiment.)

In the experimental method, 1% SLS (Sodium Lauryl Sulfate) was applied for 24 hours by dividing the unused area (control group) and the used area in the whole area, and temporarily weakened the skin barrier, After 4 hours of application, the whole bark area was measured. The transdermal water loss (TEWL) was measured using a vapometer. The measurement was performed once per site, and the unit of measurement was g / m ² h. Since the amount of transdermal water loss and the degree of skin barrier damage are proportional, the lower the measured value, the better the skin barrier improvement effect.

 (Unit: wt%) shape ingredient Example 1
(Plant-derived liposome) Example 7 Example 8 Example 9 Comparative Example 1
(Ball liposome) Comparative Example 2
(Pseudotyped liposomes) Awards
(A) Purified water To 100 To 100 To 100 To 100 To 100 To 100 Natural moisturizing factor mixture 10 10 10 10 - - Stratum-like components (bentonite, olive oil decyl ester, citric acid, lactic acid and squalene) 2 - 0.01 5 - - glycerin 25 25 25 25 25 Hydrogenated lecithin 4 4 4 4 4 2 Paid
(B) Caprylic / capric triglyceride 15 15 15 15 15 - Triglyceride - - - - - 4 Cholesterol ester - - - - - 10 Ceramide - - - - - 5 Squalene - - - - - One adding
(C) Free fatty acid - - - - - 2 Ceramide 2 2 2 2 - 2 Phytosterol One One One One - - Vegetable oils (blackcurrant seed oil, sunflower seed oil unsubstantiated, balloon vine oil) 2 2 2 2 - -

division Control group Example 1 Example 7 Example 8 Example 9 Comparative Example 1 Comparative Example 2 TEWL (g / m ² h) -2.7 -17.8 -8.8 -10.5 X -5.8 -6.9

※ TEWL: Transepidermal water loss (transdermal water loss)

As shown in FIG. 8, in the case of Example 1, the amount of transdermal water loss was statistically significant after 4 hours from the unused portion of the test product (control group), Comparative Examples 1 and 2, and Examples 7 and 8. (p < 0.05)

Thus, Examples 1 and 8 confirmed synergistic effects on the skin barrier improvement effect immediately damaged compared to the control group, the Comparative Examples 1, 2 and 7.

(2) pH buffering effect

The pH buffering effect of Example 1 and Comparative Example 1 was confirmed by experiments in order to confirm the pH buffering effect of the vegetative liposomes containing the stratum corneum-like component.

The ability of any solution or skin to buffer is an essential component of physiological barriers, which refers to the ability to maintain the pH of an existing contact with an acidic material. The buffer capacities of Example 1 and Comparative Example 1 were tested by adding citric acid.

As shown in FIG. 9, it was found that the pH of Comparative Example 1 was reduced by two stages as soon as citric acid was added, while Example 1 maintained a stable pH in the stages 4 to 6.

Thus, Example 1 containing the stratum corneum-like component has a strong buffering capacity at the characteristic pH of the skin.

[Experimental Example 5]

Reduction of skin irritation and liposome stability according to the content of vegetable oil

In order to confirm the skin irritation mitigation effect of the plant-derived liposome according to the content of the vegetable oil, plant-derived liposomes were prepared at the composition ratios shown in Table 8 below.

Experimental method was to treat skin with 1% SLS (Sodium Lauryl Sulfate), which induces skin irritation, and then apply skin lipase to plant skin with different vegetable oil content. And the results are summarized in Table 9 below.

The L * a * b system uses a Minolta Chromameter CR 400 (L * a * b color system) to measure the redness of the skin. The a * value (green - Red) is increased.

(Unit: wt%) shape ingredient Example 1
(Plant-derived liposome) Example 10 Example 11 Example 12 Example 13 Example 14 Comparative Example 2
(Pseudotyped liposomes) Awards
(A) Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 Natural moisturizing factor mixture 10 10 10 10 - - - Stratum-like components (bentonite, olive oil decyl ester, citric acid, lactic acid and squalene) 2 2 2 2 2 2 - glycerin 25 25 25 25 25 25 Hydrogenated lecithin 4 4 4 4 4 4 2 Paid
(B) Caprylic / capric triglyceride 15 15 15 15 15 15 - Triglyceride - - - - - - 4 Cholesterol ester - - - - - - 10 Ceramide - - - - - - 5 Squalene - - - - - - One adding
(C) Free fatty acid - - - - - - 2 Ceramide 2 2 2 2 - - 2 Phytosterol One One One One - - - Vegetable oils (blackcurrant seed oil, sunflower seed oil unsubstantiated, balloon vine oil) 2 0 0.1 0.5 5 10 -

division Example 1 Example 10 Example 11 Example 12 Example 13 Example 14 Comparative Example 2 Skin irritation mitigation
(Mean? Chromameter readings (a *)) One 1.4 1.3 1.14 0.93 0.87 1.32 Liposome stability ○ ○ ○ ○ ○ △
(Oil bare) △
(Oil bare)

As shown in FIG. 10, in Example 10 and Comparative Example 2, the effect of alleviating skin irritation was not significant. In Example 11, the effect of alleviating skin irritation began to appear. In Examples 1 and 12 to 14, . In particular, in Examples 1 and 12 to 13, the skin irritation mitigation effect was excellent. However, in Example 14, the effect of alleviating the skin irritation was shown, but it was confirmed that the liposome stability was slightly unstable when the vegetable liposomes were slightly oiled.

[Experimental Example 6]

Improvement of skin barrier and evaluation of moisturizing effect of products containing plant-based liposome

Lotion (Formulation Example 1) and cream (Formulation Example 2) were prepared in order to confirm skin barrier improvement and moisturizing effect of vegetable liposome according to the content of moisturizing vegetable oil of a product containing plant-derived liposome. The specific structure thereof is described in Tables 10 and 11 below.

[Formulation Example 1]

Lotion manufacturing

division ingredient Content (% by weight) Plant-based liposome Example 1 2.0 Paid Cetyl phosphate 1.3 Glyceryl stearate 1.0 Cetyl alcohol 4.5 Sheer butter 1.5 Butyleneglycol dicaprylate / dicaprate 5.0 Cetyl hexanoate 5.0 Pentaerythritol tetrahexyl hexanoate 2.0 Dimethicone 2.0 Awards Purified water To 100 Propanediol 10.0 glycerin 5.0 Allantoin 0.1 Dipotassium glycyrrhizate 0.1 Disodium iodide 0.02 Increment Polyacrylate-13 0.5 adding 1,2-hexanediol 1.5 Spices 0.2 Sum 100.0

[Formulation Example 2]

Cream manufacturing

division ingredient content(%) Plant-based liposome Example 1 2.0 Awards Purified water To 100 Disodium iodide 0.02 glycerin 5.0 Propanediol 10.0 Dipotassium glycyrrhizate 0.1 Carbomer 0.06 Paid Butyleneglycol dicaprylate / dicaprate 5.0 Stearic acid 1.0 Behenyl alcohol 2.3 C14-22 alcohol, C12-20 alkyl glucoside 1.0 Pentaerythrityl tetraisostearate 6.0 Cetyl hexanoate 3.0 Cyclopentasiloxane, cyclohexasiloxane 3.0 Neutralization Tromethamine 0.04 Increment Polyacrylate-13 0.6 adding 1,2-hexanediol 1.5 Spices 0.12 Sum 100.0

(1) Percutaneous water loss measurement for adults

In order to evaluate the efficacy of the product against the skin barrier improvement effect of Formulation Example 1 and Formulation 2 containing plant-derived liposomes, the experiment for measuring the transdermal water loss was conducted for adults.

In the experimental method, 1% SLS (Sodium Lauryl Sulfate) was applied for 24 hours by dividing the unused part and the used part in the whole area, temporarily weakening the skin barrier, The test product was allowed to coat for two weeks.

Percutaneous water loss was measured using a vapometer before the use of the test product (before SLS application), after removal of the patch, 1 day, 7 days and 14 days after using the test product. The measurement was carried out once per site and the unit of measurement was g / m ² h. Since the amount of transdermal water loss and the degree of skin barrier damage are proportional, the lower the measured value, the better the skin barrier improvement effect.

As shown in FIG. 11, in the case of Formulation 1, the amount of transdermal water loss showed a statistically significant difference between the site of use and the site of use 1 day, 7 days, and 14 days after the product was used. (p < 0.05)

Also, as shown in FIG. 12, in the case of Formulation Example 2, the amount of transdermal water loss showed a statistically significant difference after 1 day and 14 days after use of the product, compared to the unused portion of the test product. (p < 0.05)

Thus, the test products, Formulation Example 1 and Formulation Example 2, were judged to be products that could be used to improve the damaged skin barrier by two weeks of use.

(2) Measuring duration of moisture for 120 minutes for infants

This study was conducted to evaluate the effect of moisturizing duration of 120 minutes after applying the product of Formulation Example 2 to the skin of 5 ~ 6 year old infants.

The skin moisturizing power was measured using a Corneometer CM825 (Courage-Khazaka eletronic GmbH, Germany). Measurements were taken before, immediately after, and 120 minutes after use of the test product. Corneometer probe was contacted to the skin 3 times through the sensor, and the average value was used as the evaluation value of skin moisture. The unit is expressed as a unit constant, A.U, the amount of skin moisturizing is proportional to the measured value.

The subjects were 21 boys and 13 girls and 8 girls. The mean age was 5.14 years. Formulation Example 2 The skin moisture content was measured (see FIG. 13) after use, and it was significantly increased (p <0.05) both immediately after use and after 120 minutes of use compared to before use (p <0.05) , And 120 minutes after use (p <0.05) (see Fig. 14).

[Formulation Example 3]

Preparation of Formulations for Confirming the Stability of Cosmetic Composition

To confirm the stability of the plant-derived liposome-containing cosmetic composition, a formulation (solubilized formulation, essence formulation, cream formulation) containing plant-derived liposome was prepared according to Formulation Examples (3-1) to (3-3).

(3-1) Preparation of Solubilized Formulations

1) The water phase and the usable image were uniformly mixed and dissolved respectively.

2) An aqueous image was added to the water phase and mixed and solubilized.

3) Addition I was solubilized into a plant-containing liposome-containing phase of Example 1, followed by addition and mixing with addition II.

division ingredient Content (% by weight) Addition I Example 1 0.5 Awards Purified water To 100 glycerin 10-25 Dipropylene glycol Betaine D-Panthenol Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Available image PIGGY-60 Hydrogenated Castor Oil 0 to 2 PIGGY-40 Hydrogenated Castor Oil 0 to 2 Polyhydric alcohol 0 to 10 Spices Suitable amount ethanol 0 to 20 Addition II Preservative Suitable amount Other additives Suitable amount

(3-2) Preparation of Essence Formulation

1) The water phase and the oil phase were warmed and homogeneously mixed and dissolved respectively.

2) The oil phase was added to the aqueous phase at 75 캜, followed by mixing and emulsification.

3) Addition I was added at 50 ° C to a plant-containing liposome-containing phase and mixed, followed by addition II.

division ingredient Content (% by weight) Addition I Example 1 0.5 Awards Purified water To 100 Cetearyl-6 olivate 0.1 to 3 glycerin 10-25 1,2-propanediol Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Paid FAGE -100 stearate 0.1 to 1 Glyceryl stearate 0.1 to 1 Polysorbate 60 0.1 to 1 Cetyl alcohol 0.1 to 1 Behenyl alcohol 0.1 to 1 Squalene 5-20 Tocopheryl acetate 0.1 to 0.5 Addition II incense Suitable amount Preservative Suitable amount Other additives Suitable amount

(3-3) Preparation of cream formulations

1) The water phase and the oil phase were warmed and homogeneously mixed and dissolved respectively.

2) The oil phase was added to the aqueous phase at 75 ° C, and the mixture was emulsified

3) Addition I was added at 50 ° C to a plant-containing liposome-containing phase and mixed, followed by addition II.

division ingredient Content (% by weight) Addition I Example 1 0.5 Awards Purified water To 100 glycerin 10-25 Betaine Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Paid FAGE -100 stearate 0.1 to 2 Glyceryl stearate 0.1 to 2 Polysorbate 60 0.1 to 2 Stearic acid 0.1 to 2 Cetearyl alcohol 0.1 to 2 Capric caprylic triglyceride 10 to 30 Tocopheryl acetate 0.1 to 0.5 Addition II incense Suitable amount Preservative Suitable amount Other additives Suitable amount

[Experimental Example 7]

Confirmation of stability of cosmetic composition containing botanical liposomes

To confirm the formulation stability, the solubilization formulations, essence formulations and cream formulations of Formulation Examples (3-1) to (3-3) were stored for 12 weeks at 4 ° C, 30 ° C, 45 ° C, , And color, odor and formulation changes were observed through visual evaluation and sensory evaluation. The results are shown in Tables 15 to 17 below. At this time, the degree of change in color and coating form was classified and evaluated according to the following evaluation criteria.

<Evaluation Criteria>

Variation No: ○ / slight change: Δ / significant change: X

lapse
time Formulation Example (3-1) Formulation for solubilization Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

lapse
time Formulation Example (3-2) Essence formulation Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

lapse
time Formulation Example (3-3) Formulation of cream Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

As a result, as shown in Tables 15 to 17, it was confirmed that both the solubilized formulation, the essence formulation and the cream formulation had excellent stability under high temperature and daylight conditions.

[Experimental Example 8]

Confirm skin irritation of cosmetic composition containing botanical liposomes

In order to confirm the skin irritation of the cosmetic composition containing plant-derived liposome, the skin irritation of the cream formulation of Formulation Example 2 was evaluated.

Specifically, a predetermined amount (20 μl) of a cream formulation containing concentration of plant-derived lipase in the dosage form (3-3) in a concentration of 5 × 20 cm in the back region was administered to adult subjects for 24 hours After the patches were removed, the skin condition changes were visually observed after 1 hour and 24 hours, and the results are shown in Table 12. At this time, the degree of change of the skin condition was classified and evaluated according to the following skin condition evaluation standard.

<Criteria for skin condition>

0: no change / 1: extremely slight change / 2: slight change / 3: slight change

4: Severely changed / 5: Extremely changed

Subject (sex, skin type) Elapsed time Skin condition according to vegetative liposomal contents 2.0 wt% 1 (male, neutral) 1 hours 0 24 hours 0 2 (female, neutral) 1 hours 0 24 hours 0 3 (female, neutral) 1 hours 0 24 hours 0 4 (female, neutral) 1 hours 0 24 hours 0 5 (female, neutral) 1 hours 0 24 hours 0 6 (female, neutral) 1 hours 0 24 hours 0 7 (male, neutral) 1 hours 0 24 hours 0 8 (female, neutral) 1 hours 0 24 hours 0 9 (female, neutral) 1 hours 0 24 hours 0 10 (female, neutral) 1 hours 0 24 hours 0 11 (female, neutral) 1 hours 0 24 hours 0 12 (female, neutral) 1 hours 0 24 hours 0 13 (female, neutral) 1 hours 0 24 hours 0 14 (female, neutral) 1 hours 0 24 hours 0 15 (female, neutral) 1 hours 0 24 hours 0 16 (female, neutral) 1 hours 0 24 hours 0 17 (female, neutral) 1 hours 0 24 hours 0 18 (female, neutral) 1 hours 0 24 hours 0 19 (female, neutral) 1 hours 0 24 hours 0 20 (female, neutral) 1 hours 0 24 hours 0 21 (male, neutral) 1 hours 0 24 hours 0 22 (female, neutral) 1 hours 0 24 hours 0 23 (female, neutral) 1 hours 0 24 hours 0 24 (female, neutral) 1 hours 0 24 hours 0 25 (female, neutral) 1 hours 0 24 hours 0 26 (female, neutral) 1 hours 0 24 hours 0 27 (female, neutral) 1 hours 0 24 hours 0 28 (female, neutral) 1 hours 0 24 hours 0 29 (female, neutral) 1 hours 0 24 hours 0 30 (female, neutral) 1 hours 0 24 hours 0

As a result, as shown in Table 18, it was confirmed that no skin irritation was observed even in cream formulations containing plant-derived liposomes.

<110> CELLTRION INC. <120> Compositions for Moisturizing Skin Containing Vegetable Vernix          Ingredients <130> CPD2017044KR <150> KR 10-2016-0114684 <151> 2016-09-06 <160> 14 <170> KoPatentin 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> GAPDH forward primer <400> 1 aacgaatttg gtcgaacagc 20 <210> 2 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> GAPDH reverse primer <400> 2 tgaggaggga ttcagtg 17 <210> 3 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HAS-1 forward primer <400> 3 gaatccttcg actgggtcct cag 23 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS-1 reverse primer <400> 4 gacacgacgc agacaagatg 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS-2 forward primer <400> 5 gcaaacctat ttgaccatcg 20 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> HAS-2 reverse primer <400> 6 gccacttctg tctactcagt g 21 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS-3 forward primer <400> 7 gaggactggt accatcagaa 20 <210> 8 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> HAS-3 reverse primer <400> 8 cgatggtagt tgtttagacc g 21 <210> 9 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Filaggrin forward primer <400> 9 gttacaattc caatcctgtt gttttc 26 <210> 10 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Filaggrin reverse primer <400> 10 cgttgcataa taccttggat gatc 24 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Involucrin forward primer <400> 11 gtgggggaga gagggaatta 20 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Involucrin reverse primer <400> 12 ctcacctgag gttgggattg 20 <210> 13 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Aquaporin-3 forward primer <400> 13 tcattatgct gaggattt 18 <210> 14 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Aquaporin-3 reverse primer <400> 14 ggcctcccat ctccttca 18

Claims (12)

A natural moisturizing factor mixture, a stratum corneum-like component, and a vegetable oil as an active ingredient.
The method according to claim 1,
5 to 15 wt% of a natural moisturizing factor mixture, 0.01 to 3 wt% of a stratum corneum-like component, and 0.01 to 10 wt% of a vegetable oil, based on the total weight of the composition.
The method according to claim 1,
Wherein the natural moisturizing factor mixture is selected from the group consisting of sodium pyrolidonecarboxylate, sodium lactate, urea, serine, glycine, arginine, glutamic acid, tyrosine and alanine By weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the stratum corneum-like component comprises bentonite, olive oil decyl ester, citric acid, lactic acid and squalane.
5. The method of claim 4,
Wherein the stratum corneum-like component further comprises at least one member selected from the group consisting of glycerin, hydrated silica, chlorella bulguris and sea salt.
The method according to claim 1,
The vegetable oil may be selected from the group consisting of blackcurrant seed oil, sunflower seed oil unsaponifiable oil, balloon vine oil, plant derived tocopherol, sunflower seed oil, rosemary leaf oil, rice bran oil, phytosqualene, grape seed oil, jojoba oil, Wherein the cosmetic composition is at least one selected from the group consisting of polyvinylpyrrolidone and polyvinylpyrrolidone.
The method according to claim 1,
Wherein the composition further comprises at least one selected from the group consisting of triglyceride, ceramide, and phytosterol.
The method according to claim 1,
Wherein the composition has a function similar to that of natural wild-type.
The method according to claim 1,
Wherein the composition enhances the skin moisturizing effect by strengthening the skin barrier.
The method according to claim 1,
Wherein the composition is adapted to calm skin irritation.
The method according to claim 1,
Wherein the composition is in the form of a liposome.
The method according to claim 1,
The composition can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Lotion, cleansing cream, body lotion, and body cleanser. The cosmetic composition for skin moisturizing according to claim 1,

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