KR20030085941A - Liposome or nanoemulsion containing dipalmitoyl hydroxyproline and soy isoflavone and a cosmetic composition containing the liposome and/or the nanoemulsion and method thereof - Google Patents

Abstract

The present invention relates to liposomes or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones, and to cosmetic compositions containing the same, and more specifically to dipalmitoylhydroxyproline and soisoisoflavones. A cosmetic composition prepared by preparing liposomes or nanoemulsions containing% to 10%, and adding the prepared liposomes and / or nanoemulsions to the cosmetics in an amount of 0.1 to 20%, wherein the liposomes and nanoemulsion structures are active ingredients. Since it promotes skin permeation and sustained release of palmitoylhydroxyproline and soisoisoflavones, the cosmetic composition containing the same has excellent skin moisturizing power and anti-wrinkle production.

Description

Liposomes or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones and methods for preparing the same and methods for preparing the same method kind}

The present invention relates to liposomes or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones and cosmetic compositions containing them, and more particularly, dipalmitoylhydroxyproline and soisoisoflavones are encapsulated in lecithin phospholipid vesicles. (encapsulation) to create a liposome or nanoemulsion having excellent skin permeability and durability, and relates to a cosmetic composition excellent in skin moisturizing and elasticity prepared by containing it.

The method of suppressing the aging phenomenon such as skin wrinkles and skin drying caused by the decrease of elasticity of the skin and loss of moisture retention with age is one of the biggest areas of interest in cosmetics.

The aging of the skin is caused by various causes, but the most important factor is the structural change deep in the skin, which leads to dermal degradation, that is, the loss of skin activity. As the dermal tissue breaks down, the skin surface loses its original firmness and gradually loosens.

Dermal cells are located just below the epidermis and are a very sensitive layer of collagen fibers that are woven into a net-like shape, between which elastin fibers are entangled like springs to maintain the elasticity of the skin. If the net structure is consistently well-woven, the skin will remain elastic and smooth, but if the net structure of collagen and elastin is broken, the skin will lose its elasticity.

Collagen and elastin are synthesized in fibroblasts, and the activity of fibroblasts has an important effect on the state of collagen and elastin. However, with age, the activity of fibroblasts decreases, which significantly reduces the synthesis of collagen and elastin, thereby reducing the elasticity of the skin.

Therefore, conventionally, a method of uniformly mixing collagen and elastin directly into a cosmetic or preparing a cosmetic capsule and dispersing it in cosmetics has been used for the purpose of preventing skin aging by maintaining collagen and elastin in the dermal layer. However, collagen and elastin applied to the skin have to penetrate into the dermis layer in order to have a practical effect. However, in the conventional method, collagen and elastin have a low rate of absorption of the dermis and continue to operate continuously.

In order to solve the above problems, dipalmitoylhydroxyproline and soisoflavin, which are effective for the production and stabilization of collagen and elastin, are encapsulated in liposomes or nanoemulsions and are contained in cosmetics, which are effective for moisturizing and maintaining elasticity of skin. It is an object to provide a cosmetic composition.

Accordingly, it is an object of the present invention to provide liposomes and nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones effective for the production and maintenance of collagen and elastin, which are essential for moisturizing and maintaining elasticity of skin.

Still another object of the present invention is to provide a cosmetic composition containing the liposomes and / or nanoemulsions.

In order to achieve the above object, liposomes or nanoemulsions according to the present invention are characterized by containing dipalmitoylhydroxyproline and soisoisoflavones.

In order to achieve another object of the present invention, the cosmetic composition containing liposomes and / or nanoemulsions according to the present invention comprises a blend of 0.01 to 20.0% by weight of liposomes and / or nanoemulsions and 80 to 99.99% of cosmetic components. Preferably, 0.1 to 10.0% by weight of liposomes or nanoemulsions are blended with 90 to 99.9% by weight of cosmetic components.

In order to achieve the another object of the present invention, the method for preparing a cosmetic composition according to the present invention comprises the steps of dissolving and dissolving dipalmitoylhydroxyproline, caprylic / capric triglyceride and diisopropyl adipate, followed by heating;

Mixing PIG 40 hydrogenated castor oil as a solubilizing agent and mixing unsaturated lecithin, soisoisoflavone (aglycone), glycerol, and alcohol, followed by adding purified water;

Passing the mixed solution through a high pressure emulsifier (Microfluidizer) is characterized in that it comprises.

Figure 1 shows the measured particle size and distribution of the liposomes and nanoemulsion prepared by the present invention.

Hereinafter, the present invention will be described in detail.

Hydroxyproline is excellent in moisturizing ability because it can firmly bind water molecules around molecules. In addition, it is essential for collagen formation as an important compound for inhibiting the overproduction of elastinase and stabilizing the triple helix structure of collagen. Dipalmitoylhydroxyproline is used to convert palmitic acid, a type of fatty acid, to hydroxyproline. As a combined compound, it has excellent skin permeability and facilitates skin absorption of hydroxyproline, and also has a lipophilic structure, thereby preventing water loss in the outer skin layer.

Soy isoflavones bind to the receptors of the female sex hormone estrogen and function similar to estrogen. Estrogens stimulate the production of collagen and elastin. Administration of estrogen to postmenopausal women increased the amount of collagen, skin elasticity and skin thickness. However, these hormone replacement therapies have side effects that cause heart disease and uterine cancer. However, it is widely known that soy isoflavin is a phytoestrogens with no side effects, and soy foods containing large amounts of soy isoflavin are effective for preventing osteoporosis, uterine cancer and breast cancer in menopausal women.

Isoflavones can be divided into two broad classes: sugar-containing glycosides and sugar-free glycosides. Since glycosides cannot penetrate the skin, sugar-free aglycone types are used in cosmetics.

The present invention focused on the functions of dipalmitoylhydroxyproline and soisoisoflavones described above, and focused on the means for containing dipalmitoylhydroxyproline and soisoisoflavones in cosmetics and effectively infiltrating them into the skin. .

As far as is known, the most suitable structures for penetrating moisture and effective bioactive ingredients into the skin in cosmetic compositions are liposomes and nanoemulsions.

It is because liposomes and nanoemulsions have excellent affinity with skin tissues, they can contain both hydrophilic and lipophilic substances, and can be controlled to release their contents slowly and continuously.

Liposomes are spherical phospholipid vesicles of self-associating colloidal particles that form spontaneously aligned structures by the interaction of the amphiphilic molecules of the hydrophilic head and the lipophilic tail. Liposomes used for drug delivery are generally closed bilayer structures, which may contain a hydrophilic material inside the water-soluble nucleus, and may contain amphiphilic or lipophilic materials inside the lipid bilayer.

Nanoemulsion is a vesicle with an average diameter of 30-1,000 nm of particles formed by a monomolecular layer of phospholipid. O / W type in which oil is dispersed in water has a fat-soluble core inside.

The present invention uses unsaturated lecithin to form phospholipid membranes of liposomes and nanoemulsions. Unsaturated lecithin emulsifies the stratum corneum and promotes skin penetration of minor components.

In addition, the liposomes and nanoemulsions of the present invention contain diisopropyl adipate, which is an ester compound. Diisopropyl adipate is effective for stabilizing liposomes or nanoemulsions by dissolving dipalmitoylhydroxyproline to make them stable in liposomes or nanoemulsions.

Liposomes or nanoemulsions according to the present invention contain dipalmitoylhydroxyproline and isoisoflavones in an amount of 0.01 to 10% by weight.

In addition, liposomes or nanoemulsions according to the present invention contain 1 to 5% by weight of lecithin and 1 to 5% by weight of a solubilizing agent with respect to the total weight of liposomes or nanoemulsions, and 20 to 5 to caprylic / capric triglycerides, glycerol, alcohols. 50% by weight is mixed.

Cosmetic compositions containing liposomes and / or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones according to the present invention comprise 0.01 to 20.0% by weight liposomes and / or nanoemulsions and 80 to 99.99% cosmetic ingredients. As this compound, 90 to 99.9% by weight of the cosmetic component is preferably blended with 0.1 to 10.0% by weight of liposomes or nanoemulsions.

Next, the present invention will be described in detail with examples of preparation of liposomes and nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones, and formulation examples of cosmetics containing the liposomes and nanoemulsions. However, the present invention is not limited thereto.

Example 1

Method for preparing liposomes and / or nanoemulsions

In the preparation of liposomes or nanoemulsions, known high pressure homogenization using a microfluidizer is used.

Specific manufacturing examples are as follows.

First, 0.5% by weight of dipalmitoylhydroxyproline, 8.0% by weight of caprylic / capric triglyceride, and 1.0% by weight of diisopropyl adipate were mixed and dissolved in a composition as shown in Table 1, followed by cooling. To this, 2.0% by weight of Fiji 40 Hydrogenated Castor Oil was mixed as a solubilizing agent, 5.0% by weight of unsaturated lecithin, 0.06% by weight of isoflavone (aglycone), 15.0% by weight of glycerol, and 15.0% by weight of alcohol. Was added to make the weight 100%. The mixed solution was passed through a high pressure emulsifier (Microfluidizer) five times under a pressure of 1,200 bar to obtain liposomes and nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones.

Table 1: Liposomes and Nanoemulsion Components

ingredient weight% A Dipalmitoyl hydroxyproline 0.5 Caprylic / Capric Triglycerides 8.0 Diisopropyl Adipate 1.0 B Fiji 40 Hydrogenated Cast Oil 2.0 Unsaturated lecithin 5.0 Soy isoflavones (aglycones) 0.06 Glycerol 15.0 Alcohol 15.0 Purified water To 100.0

After storing the liposomes and nanoemulsion prepared in Preparation Example 1 in a 25 ℃ thermostat, Autosizer Lo-C MALVERN of a laser light scattering system (Laser light scattering system). The distribution and size of the particles were measured using the UK and shown in FIG. 1.

The size of liposomes or nanoemulsions prepared by the present invention is 30 ~ 130nm, the average size is 51.6nm fine particles with excellent skin penetration.

Formulation Example

Hereinafter, the configuration of the cosmetics containing the liposomes and / or nanoemulsions containing the dipalmitoylhydroxyproline and the isoflavone prepared in Example 1 will be described in more detail. However, the present invention is not limited to the following formulations of nutrition cream, nourishing cosmetics, and soft cosmetics, and can also be formulated into astringent cosmetics, eye cream, massage cream, cleansing cream, cleansing foam, cleansing water, pack and the like.

Formulation Example 1

Preparation of Nutritional Cream

In the composition of Table 2, phase A, which is a moisturizing component, is heated to 75 ° C. and stirred at a constant speed, phase B which provides a protective oil film on the skin surface is heated to 70 ° C., stirred at a constant speed, and phase B is phase A It was emulsified while slowly added to. Phase C was added to (A + B), then slowly cooled, filtered and aged to fill the vessel.

TABLE 2 Formulation Examples and Comparative Examples of Nutritional Cream

Ingredient Name Formulation example (% by weight) Comparative example (wt%) A 1. Carboxy vinyl polymer 0.13 0.13 2.butylene glycol 5.0 5.0 3.glycerine 3.0 3.0 4.propylene glycol 1.5 1.5 5 purified water to 100.0 to 100.0 B 6.Beswax 2.0 2.0 7.petrolatum 7.0 7.0 8.Fluid Paraffin 10.0 10.0 9.Polysorbate 60 2.0 2.0 10.Phytoscualan 3.0 3.0 11.Solbitan sesquioleate 2.5 2.5 12.Tocopheryl Acetate 0.1 0.1 13.triethanolamine 0.13 0.13 C 14.Liposomes and nanoemulsions containing dipalmitoylhydroxyproline, soisoisoflavones 3.0 - 15.preservative 0.4 0.4 16 yellow yellow Quantity Quantity 17.Spice 0.2 0.2

Formulation Example 2

Preparation of Nutrients

In the composition shown in Table 3, phase A, which is a moisturizing component, is heated to 75 ° C. and stirred at a constant speed, phase B which provides a protective oil film on the skin surface is heated to 70 ° C., stirred at a constant speed, and phase B is phase A It was emulsified while slowly added to. Phase C was added to (A + B), then slowly cooled, filtered and aged to fill the vessel.

Table 3 Formulation Examples and Comparative Examples of Nutrients

Ingredient Name Formulation example (% by weight) Comparative example (wt%) A 1. Carboxy vinyl polymer 0.13 0.13 2.butylene glycol 5.0 5.0 3.glycerine 3.0 3.0 4.purified water to 100.0 to 100.0 B 5.Fluid Paraffin 5.0 5.0 6.Stearic acid 0.4 0.4 7.Tocopheryl Acetate 3.0 3.0 8.Macadamia Oil 2.0 2.0 9.Phytoscualan 3.0 3.0 10.Polysorbate 60 1.5 1.5 11.Solbitan sesquioleate 1.0 1.0 12.Triethanolamine 0.13 0.13 C 13.Liposomes and nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones 3.0 - 14. Preservative 0.4 0.4 15.Yellow 5 Quantity Quantity 16.Spice 0.2 0.2

Formulation Example 3

Manufacturing method of flexible cosmetics

A, B, C, D are sequentially added to the composition shown in Table 4 below, followed by stirring at a constant speed to mix.

Table 4 Formulation Examples and Comparative Examples of Softener

Ingredient Name Formulation example (% by weight) Comparative example (wt%) A 1. Carboxy vinyl polymer 0.1 0.1 2.triethanolamine 0.1 0.1 3.1,3-butylene glycol 6.0 6.0 4.glycerine 4.0 4.0 B 5.Tocopheryl Acetate 0.05 0.05 6.Hydrovited Castor Oil 0.3 0.3 7.ethanol 15.0 15.0 8.Fragrance, preservative Quantity Quantity C 9.purified water to 100 to 100 D 10.Liposomes and Nanoemulsions Containing Dipalmitoylhydroxyproline and Soisoisoflavones 3.0 -

-Efficacy investigation test of the cosmetics according to the present invention-

For each of the contents prepared according to the three formulation examples and comparative examples, the measurement and comparative evaluation of the moisturizing ability and general sensory test for 10 women aged 25-45 years.

[Test Example 1] Moisturizing effect test

The skin moisturizing effect of the liposomes containing the dipalmitoylhydroxyproline and the isoflavone and the cosmetics containing the nanoemulsion were measured.

In a room without air flow at 25 ° C., relative humidity of 45%, 10 healthy women were placed on the right, left poles, and 5 cm below, with the examples on the right and the comparative examples on the left. Formulated according to Table 1 and applied twice a day for 7 days at the position of 5cm below the forearm and the lower arm of the arm, using Corneometer CM 825 (CK Courage & KhazakaGermany) to change the electrical conductivity according to the epidermal moisture content from 0 to 150 Moisturizing effect was quantified by measuring the moisture content in the skin five times in a row and the average value was measured as a moisturizing effect. The experimental results are shown in Table 5 below.

[Table 5] Skin moisturizing effect

product Prescription Example Comparative Example 0 2 days 4 days 7 days 0 2 days 4 days 7 days Subject 1 43 51 74 72 40 42 54 61 Subject 2 35 59 58 82 40 70 59 70 Subject 3 30 61 62 67 35 53 54 61 Subject 4 29 46 56 67 22 46 42 48 Subject 5 56 74 82 93 56 72 72 75 Subject 6 42 56 69 67 38 50 58 59 Subject 7 35 56 75 74 45 53 70 86 Subject 8 54 62 67 83 45 46 46 70 Subject 9 46 61 78 88 34 51 53 72 Subject 10 45 50 54 59 42 53 56 58 Average 42 58 68 75 40 54 56 66

As a result of the test, the Examples of the present invention were measured immediately after the first use of 42, after 2 days of use 58, after 4 days of 68, 7 days after 75 days of use, the average amount of water was measured, showing an improved effect compared to the Comparative Example As it is used continuously, the effect of increasing the moisturizing effect was more pronounced compared to the cosmetic of the comparative example.

Test Example 2 Skin Safety Experiment

Skin safety experiments were carried out on cosmetics containing liposomes and / or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones.

Ten subjects (age distribution 25-45 years old) were subjected to a patch test on the upper arm using Haye's Test Chamber. Psoriasis, eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, and antihistamines, were excluded from this study.

After wiping the test site with 70% ethanol and drying, the sample and the comparative example were formulated according to the above Table 3 and dropped into the chamber by 15 µg, respectively, and then fixed on the upper arm, which is the test site. After removing the patch by applying for 24 hours, mark the test site with a marking pen and observe the test site after 24 hours and 48 hours, respectively. Determination is carried out 24 hours and 48 hours after patch removal. The judgment was made according to the regulations of the International Contact Dermatitis Research Group (ICDRG) of Table 6. The experimental results are shown in Table 7 below.

[Table 6] Judgment Criteria for International Contact Dermatitis Research Group

sign Criteria evaluation Mean score ± ++++++- Doubtful or slight reaction and erythemaErythema + IndurationErythema + Induration + VesicleErythema + Induration + Bullae Stimulus stimulus non stimulus 0 to 0.91.0 to 2.93.0 to 4.95.0 or more

Table 7 Skin Safety

Elapsed time Example Comparative Example 24 hours 0 0 48 hours 0 0

As a result of skin safety test, liposomes containing dipalmitoylhydroxyproline and soisoisoflavones and / or nutritional creams containing nanoemulsions (Formulation Example 1) did not show irritation in all the subjects with an average irritation degree of 0 It can be seen that liposomes and nanoemulsions containing hydroxyproline and soisoisoflavones are safe substances without irritation to the skin.

[Test Example 3] Moisturizing effect sensory test

The moisturizing effect of the nutritional cream, the nourishing cosmetics, and the softening cosmetics prepared according to the above-described formulation examples and comparative examples was compared and evaluated five-point scales after 10 days of use for 10 women aged 25 to 45 years. Regarding the question of whether skin moisturization has improved, it is criticized as 'not at all-1', 'not at most-2', 'normal is-3', 'yes-4' and 'very yes-5' The point of application was comparatively divided into just after using the product once and after using it twice a day for 7 days. The results are shown in Table 8.

Table 8 Comparative sensory data of the present invention (skin moisturizing effect)

product Skin moisturizing effect Immediately after use After 7 days of use Nutrition Cream Formulation example 3.9 4.5 Comparative formulation example 3.8 4.2 Lotion Formulation example 3.7 4.2 Comparative formulation example 3.5 4.0 skin Formulation example 3.6 4.1 Comparative formulation example 3.5 3.8

Test Example 4 Elasticity Sensory Test

The elasticity effect on the nutrition cream, nourishing cosmetics, and supple cosmetics prepared according to the above-described formulation examples and comparative examples was compared and evaluated five-point scale after use twice a day for 10 women aged 25 to 45 years. Regarding the question of whether skin elasticity has improved, it is criticized as 'not at all-1', 'not at most-2', 'normal is-3', 'yes-4' and 'very yes-5'. The point of application was comparatively divided into just after using the product once and 7 days after using it twice a day. The results are shown in Table 9.

Table 9 Comparative sensory data of the present invention (elasticity increasing effect)

product Elasticity increasing effect Immediately after one use After 7 days of use Nutrition Cream Formulation example 3.2 4.2 Comparative formulation example 3.1 3.3 Lotion Formulation example 3.2 3.9 Comparative formulation example 3.2 3.3 skin Formulation example 3.1 3.7 Comparative formulation example 3.0 3.2

Tables 8 and 9 evaluate the product effects perceived by the subject and perceived by sensory (sensation). Thus, the numerical value evaluated by the sensory (sensation) of the subject was digitized by taking the average of 10 points out of 5 points. The higher the value of the subjects was, the higher the satisfaction was, and the satisfaction would soon indicate that the product was excellent.

For example, the nutrition cream prepared by Formulation Example 1 of the present invention, the nutrition cream prepared by Formulation Example 1 is a sensory evaluation on the moisturizing efficacy immediately after one use and twice a day after 7 days of use, respectively As 3.9 and 4.5, excellent effects were obtained by continuous use compared to sensory evaluation 3.8 and 4.2 for the moisturizing effect of the nourishing cream prepared by Comparative Formulation Example 1.

The sensory evaluation of the increase in elasticity is that the nutrition cream of Comparative Example 1 is 3.1 and 3.3, respectively, immediately after one use and twice a day after 7 days of use. It can be seen that the cosmetics containing liposomes and / or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones have excellent effects on skin moisturizing ability and elasticity increasing effect.

As described above, liposomes or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones and cosmetics containing them showed more improved effects in moisturizing effect, increased elasticity and general functional usability compared to conventional comparative formulations. In particular, it showed a better effect when used continuously.

As such, the present invention is effective to maintain elastic and healthy skin by effectively acting on the skin to increase the moisturizing ability and elasticity of the skin.

Claims (29)

Liposomes containing dipalmitoylhydroxyproline and soisoisoflavones The liposome according to claim 1, wherein the particle size is 30 to 130 nm. The liposome according to claim 1, wherein the dipalmitoyl hydroxyproline and the isoflavone are contained in an amount of 0.01 to 10% by weight. The liposome according to claim 1, wherein the soisoisoflavone is an isoflavone in the form of aglycoside aglycone. The liposome according to claim 1, wherein the phospholipid forming the membrane of the liposome is unsaturated lecithin. The method according to claim 1, wherein 1 to 5% by weight of lecithin and 1 to 5% by weight of solubilizer are contained relative to the total weight of liposomes, and 20 to 50% by weight of caprylic / capric triglyceride, glycerol and alcohol are mixed. Feature liposomes The liposome according to claim 6, wherein the solubilizer is Fiji 40 Hydrogenated Castor Oil. The liposome according to claim 6, further comprising diisopropyl adipate. Nanoemulsions containing dipalmitoylhydroxyproline and isoisoflavones The nanoemulsion according to claim 9, wherein the particle diameter is 30 to 130 nm. 10. The nanoemulsion of claim 9, wherein the dipalmitoylhydroxyproline and the isoflavone are contained in an amount of 0.01 to 10% by weight. 10. The nanoemulsion of claim 9, wherein the isoisoflavone is isoflavone in the form of aglycoside aglycone. 10. The nanoemulsion of claim 9, wherein the phospholipid forming the membrane of the nanoemulsion is unsaturated lecithin. The method according to claim 9, comprising mixing 1 to 5% by weight of lecithin and 1 to 5% solubilizer with respect to the total weight of the nanoemulsion, and mixing 20 to 50% of caprylic / capric triglyceride, glycerol and alcohol. Nanoemulsion characterized 15. The nanoemulsion of claim 14 wherein the solubilizer is Fiji 40 Hydrogenated Castor Oil. 15. The nanoemulsion of claim 14 further comprising diisopropyladipate. Cosmetic composition containing liposomes and / or nanoemulsions containing dipalmitoylhydroxyproline and soisoisoflavones The cosmetic composition according to claim 17, wherein the liposome and / or nanoemulsion is contained in an amount of 0.01 to 20.0 wt%. The cosmetic composition according to claim 18, wherein the liposome and / or nanoemulsion is contained in an amount of 0.1 to 10.0% by weight. 18. The cosmetic composition according to claim 17, wherein the formulation is a nourishing cream, nourishing cream, softening cream, astringent cream, eye cream, massage cream, cleansing cream, cleansing water, essence, pack. 18. The cosmetic composition according to claim 17, wherein the liposome or nanoemulsion has a particle diameter of 30 to 130 nm. The cosmetic composition according to claim 17, wherein the liposome or nanoemulsion contains dipalmitoylhydroxyproline in an amount of 0.001 to 5% by weight of the total weight of the liposome and the nanoemulsion. The cosmetic composition according to claim 17, wherein the liposome or nanoemulsion contains soiisoflavones in an amount of 0.001 to 5% by weight of the total weight of the liposome and the nanoemulsion. 18. The cosmetic composition according to claim 17, wherein the isoisoflavone is isoflavone in the form of aglycoside aglycone. 18. The cosmetic composition according to claim 17, wherein the phospholipid forming the membrane of the liposome or nanoemulsion is unsaturated lecithin. 18. The method according to claim 17, wherein the liposomes and nanoemulsions comprise 1 to 5% by weight of lecithin, 1 to 5% solubilizer relative to the total weight of liposomes and nanoemulsions, and caprylic / capric triglycerides, glycerol, alcohols. Cosmetic composition characterized by mixing 20-50% The cosmetic composition according to claim 26, wherein the solubilizer is Fiji 40 Hydrogenated Castor Oil. The cosmetic composition according to claim 26, further comprising diisopropyl adipate. Heating, dissolving and cooling dipalmitoylhydroxyproline, caprylic / capric triglyceride, and diisopropyl adipate; Mixing PIG 40 hydrogenated castor oil as a solubilizing agent and mixing unsaturated lecithin, soisoisoflavone (aglycone), glycerol, and alcohol, followed by adding purified water; Method of producing a cosmetic composition comprising the step of passing the mixed solution through a high pressure emulsifier (Microfluidizer)