KR20170141991A - Skin protective cosmetic composition for stabilizing vitamin c containing bergenin and fullerene - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin protection capable of stabilizing vitamin C by containing bergenin, fullerene, and vitamin C or derivative thereof. According to the present invention, bergenin and fullerene components improve stability to the decolorization, odor, and potency of vitamin C or derivative thereof and effectively deliver vitamin C to skin, and thus, the composition is capable of enabling the biological activity of vitamin C, thereby bringing a whitening effect (e.g. tyrosinase activity hindrance), and wrinkle improvement and antioxidation effects (e.g., free radical elimination).

Description

FIELD OF THE INVENTION [0001] The present invention relates to a skin care cosmetic composition for stabilizing vitamin C, which comprises vergene and fullerene,

The present invention relates to a cosmetic composition for skin protection comprising verrenin and fullerene. More specifically, the present invention relates to a cosmetic composition for skin protection which stabilizes vitamin C or a derivative thereof, including verrenin and fullerene.

In addition to wrinkles and elasticity, the skin becomes rough, dry, and has spots, freckles, and other natural aging. Particularly, due to the destruction of the ozone layer due to environmental pollution, the quantity of ultraviolet rays is increased and sunlight accumulates to cause photoaging. Various cosmetic compositions have been studied to solve these problems. Currently, studies for improving the wrinkles of the skin are being actively carried out, and some of them are showing visible results. In particular, there have been various reports on the clinical results of removing retinol-containing cosmetic compositions from retinoids, which have been used for the improvement of skin wrinkles, spots and pigmentation in the dermatology area, I am using it.

In addition, a cosmetic composition containing vitamin C, which is essential for the collagen synthesis process, has been reported to have the effect of improving skin wrinkles, ultraviolet light blocking, and improving pigmentation such as spots / freckles / black spots (U.S. Patent Nos. 4,938,969, 4,772,591, 533,667).

In addition, vitamin C has been widely known to have a melanin-reducing effect, an antioxidative effect, and an antiviral effect. However, the cosmetic composition containing vitamin C has a useful effect as described above, but is easily oxidized by air, moisture, light, heat, metal ions, oxygen, base and the like to produce 2,3-diketo-L- 2,3-diketo-L-gulonic acid, oxalic acid, L-threonic acid, L-xylonic acid, L-Lyxonic acid, Etc., and discoloration and irregularity occur, leading to the greatest problem in stability.

Vitamin C is involved in the collagen synthesis process and promotes collagen synthesis. The polypeptide chain of collagen fibers is a chain having extension peptides (telopeptides) at both ends. It is synthesized in rough ER, They are combined with a triple helix to form procollagen molecules that are transferred to the golgi apparatus and packed into secretory vesicles and secreted by exocytosis. The degradation of telopeptides by extracellular enzymes results in tropocollagen molecules, which are then combined in the extracellular matrix to form collagen fibers.

Wherein the tropocollagen comprises glycine, proline and hydroxyproline which are the main amino acids constituting the polypeptide alpha chain, and the triple helix structure of collagen is formed by hydroxyproline. Thus, hydroxyproline, which is important for collagen synthesis, accounts for about 10% of the collagen polypeptide and exists only in animals.

This hydroxyproline is biosynthesized by hydroxylation by prolyl hydroxylase in the presence of proline and α-ketoglutarate in the presence of oxygen. In this hydroxyation process, vitamin C Acts as a cofactor, and hydroxyproproline is biosynthesized only if it is present. That is, vitamin C accelerates the activity of prolyl hydroxylase to promote the biosynthesis of hydroxyproline, thereby promoting biosynthesis of collagen as a triple helix structure, thereby improving skin wrinkles. This prolyl hydroxylase inhibits its activity by UV-A. Vitamin C also has an ultraviolet-shielding function, thereby blocking the inhibition of the activity of prolyl hydroxylase by UV-A.

Vitamin C acts differently from conventional sunscreen agents because there is no region where L-ascorbic acid can absorb on the spectral lines of UV-A and UV-B. Vitamin C has a stronger blocking effect on UV-A than UV-B and reduces erythema and edema caused by UV-B. Vitamin C, which has a variety of biological functions, can be stored once it is in the skin and can be used for a long time even if it is discontinued. In addition, vitamin C acts as a powerful bio-antioxidant in skin, blood and other tissues, and it has sugar-like structures that are easily oxidized in the form of dehydro-L-ascorbic acid structure to prevent the biosynthetic component (protein, nucleic acid, cell membrane lipid, etc.) from being disorganized or denatured by highly reactive active oxygen, free radicals and peroxides in vivo, thereby blocking the cause of skin aging In addition, vitamin C has an anti-pigmentation effect, which is achieved by inhibiting the production of melanin, which is deeply related to pigmentation. Vitamin C has the effect of inhibiting the activity of tyrosinase, which is important for the formation of melanin, and reducing oxidized melanin formed by the oxidation process to reduce melanin to reduced melanin, thereby improving melanin pigmentation.

In addition, the physiological activity of vitamin C varies. It plays an important role in the metabolism of folate metabolism and amino acid in the living body, plays a very important role in the immune system, helps the white blood cells to act on the phagocyte, promotes the migration of leukocyte during infection, inhibits the infection, Radicals, as well as increasing the biosynthesis of interferon, which is a virus-inhibiting substance, to increase the bio-resistance against various infectious diseases. Most animals biosynthesize vitamin C from glucose (glucose), and people need to supply it from outside because they lack the enzymes needed to convert glucose (glucose) into vitamin C and can not biosynthesize glucose. In addition, vitamin C is very sensitive to heat, light, moisture, and oxygen, and is unstable. Because it is water-soluble, its ability to store in body is much lower than fat-soluble vitamins. Therefore, there is a need to continue to supply food and other methods.

Vitamin C deficiency causes collagen formation, fatigue, skin roughness, low resistance to infection, and capillary hemorrhage due to degradation of collagen biosynthesis during vitamin C deficiency. And long-term deprivation may lead to serious diseases such as scurvy.

Therefore, the recommended daily dose should be ingested and a skin-protective substance such as a vitamin C-containing percutaneous absorbent should be used. When vitamin C is applied to the skin in a water-soluble state, the vitamin C is readily absorbed through the skin barrier in the non-ionized condition. Therefore, in order to maintain the non-ionized condition, the pH of the vitamin C solution must be at least 4.2. Under these conditions, when vitamin C is applied to the skin, about 15% of the applied amount can be absorbed into the skin within 48 hours.

Once the vitamin C enters the skin, it is stabilized and accumulated on the skin without being removed by water or other rubbing. The amount of vitamin C accumulated on skin is 20 to 40 times higher than that of oral administration, C is being actively studied.

However, vitamin C is very unstable in heat, light, and oxygen, and is thus exposed to the atmosphere or easily oxidized in an aqueous solution to decrease its activity, resulting in discoloration and irritation. Despite these disadvantages, vitamin C has a high physiological activity and is used in pharmaceuticals and cosmetics. In addition, many studies have been conducted and have been used to stabilize various vitamin C derivatives. For example, there is magnesium ascorbyl phosphate that stabilizes vitamin C, which is easier to convert to L-ascorbic acid form available to the human body than other derivatives, but it is difficult to absorb skin. Another derivative, ascorbyl palmitate, is readily absorbed into the skin but is difficult to convert to L-ascorbic acid form.

In addition, efforts have been made to encapsulate and stabilize vitamin C. However, the stabilization and efficacy of vitamins C are still weak, and vitamin C, which is stable and easy to absorb into the skin, is being studied.

Korean Patent Registration No. 10-0685647 relates to a composition for stabilizing vitamin C or a derivative thereof containing verrenin as an active ingredient. Korean Patent Laid-Open No. 10-2015-0051010 relates to a cosmetic composition containing natural fullerene and a method for producing the same.

The present invention Sikkim plug children effectively stabilize the vitamin C of extensive studies, the yedeok main components of bark extract bereuge Nin and consisting of C ₆₀ and C ₇₀ in order to overcome the problem of stability with vitamin C and its derivatives have The present invention has been completed.

The present invention relates to cosmetic compositions for skin protection comprising vergene, fullerene, and derivatives of vitamin C or vitamin C.

Preferably, the derivative of vitamin C is selected from the group consisting of ascorbic acid or sodium ascorbate, magnesium ascorbate, sodium ascorbyl phosphate, ascorbyl magnesium phosphate, ascorbyl acetate, ascorbyl propionate, ascorbyl palmitate and ascorbyl stearate ≪ / RTI >

Preferably, the vergene and fullerene may be included in an amount of from 0.1% by weight to 2.0% by weight based on the total weight of the cosmetic composition.

Preferably, the weight ratio of verene to fullerene may be from 3: 7 to 7: 3.

Preferably, the weight ratio of verene to furin may be 5: 5.

Preferably, the vitamin C or derivative of vitamin C may be contained in an amount of 5 to 30% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition comprises 5 to 30% by weight of a derivative of vitamin C or vitamin C, 0.1 to 2.0% by weight of vergene and fullerene, 10 to 30% by weight of a moisturizing agent, 0.01 to 0.05% by weight of a metal sequestering agent and a residual amount of a solvent.

Preferably, the verergenin and fullerene are characterized by stabilizing the vitamin C or vitamin C derivative.

The cosmetic composition according to the present invention can improve the stability of the vitamin C or its derivative against discoloration, deterioration and potency of the verenin component and the fullerene component contained in the aqueous phase component.

The cosmetic composition of the present invention containing vergene and fullerene and vitamin C or a derivative thereof effectively stabilizes vitamin C or a derivative thereof and delivers it to the skin, thereby improving the physiological activity of vitamin C, that is, the whitening effect (e.g., inhibition of tyrosinase activity) , Wrinkle-improving effect and antioxidant effect (for example, free radical scavenging ability), and the like.

In addition, the cosmetic composition according to the present invention can provide a cosmetic without cosmetic because it has a predetermined repelling force even if it does not contain a preservative.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference. The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

The present invention relates to a cosmetic composition which protects the skin by stabilizing vitamin C or a derivative thereof, including fullerene composed of verrenine and C ₆₀ and C ₇₀ .

Bergenin, 4-methoxy-2- [tetrahydro-3,4,5-trihydroxy-6- (hydroxymethyl) pyran-2-yl] -α-resorcylic acid δ- It can be represented by the general formula (1), and it can be separated or chemically synthesized in a virtue tree or the like. It is obvious to those skilled in the art that a substance isolated from a virtue tree or chemically synthesized can exhibit the same effect.

[Chemical Formula 1]

Yeokdo is a deciduous arboreous tree called Euphorbiaceae, also known as Yaodong or Red Bait, and lives in the foothills and valley of southern Korea. Keys are 10m long, but usually they are shrubs. YeDeok is commonly used as a supplement for improving gastric ulcers, gastritis and liver function.

The major component of vermicelli is vergene, which inhibits hepatotoxicity (Kim HS et al ., J Ethnopharmacol , 2000; Jan; 69 (1): 79-83; Japanese Patent Publication No. 11209282) And reduce body fat (Japanese Patent Laid-Open No. 1180869). However, it is not known that the bergrenine can contribute to the stabilization of vitamin C.

The case where the verrhynin contained in the composition of the present invention is separated from the virtue tree can be separated by the following steps:

(I) washing the yeast tree with clean water, followed by drying and pulverizing;

(B) a water- or hydric alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol), (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h) hexane, and (i) diethyl ether Extracting with an organic solvent selected from the group consisting of: And

(Iii) separating and purifying the verrenin from the extract of step (ii) using chromatography.

In the above step (i), the virtue tree refers to various organs or parts (for example, leaves, flowers, roots, stems, branches, bark and fruit etc.) of the virtue tree, and preferably stands for stems, branches, , More preferably a stem.

In the step (ii), the extraction step using an organic solvent as an extraction solvent can be carried out according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure.

On the other hand, the method of separating veregranin from the vermicelli includes not only the above-described separation method but also a conventional purification process. For example, various additional purification methods, such as a separation method using an ultrafiltration membrane having a constant molecular weight cut-off value and a separation method by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) .

The verenin of the present invention may also be produced in powder form by performing additional processes such as vacuum distillation and freeze drying or spray drying.

The same effect can be obtained even when the chemically synthesized verghenin contained in the composition of the present invention is used. In a preferred embodiment of the present invention, vergermanic monohydrate (Sigma, B6776) from Sigma is used, but it is not necessarily limited to those produced by the above manufacturer.

The fullerene used for stabilizing vitamin C of the present invention also provides a cosmetic composition comprising C60, C70 or a mixture thereof as an active ingredient.

(2)

Fleurin has reduced the soccer ball by a factor of about a factor of 100. The soccer ball is connected to 20 regular hexagons and 12 regular pentagons, and the pentagons do not face each other. This carbon soccer ball is about 7 Å in diameter. In addition to C60, there are 12 regular pentagons, but C70, C76, C78, and C84, where the number of regular hexagons was increased, were found on accidental occasions.

Flarin is strong enough to hold diamonds as small as a cage. It can also be opened to allow insertion of other materials and can be connected like a tube. Fullerene bonds strongly with carbon atoms and is less reactive with other substances. Scientists are exploring ways to utilize these properties of fullerenes as a drug delivery system for storing medicinal ingredients or for use as carriers in the body.

In addition, the use of fullerene as a conductor or superconductor mixed with various metal atoms, or a large number of fullerenes connected to each other, can be applied to new fibers or various sensors, and the utilization of this field is gradually increasing. It is expected to be the next generation of nanomaterials that will bring innovation in polymer catalysts, computer memory devices, aerospace, and the environment.

In order to remove the active oxygen that is accumulated in daily life, it is important to carry out activities to maintain mental and physical stability such as regular living, sleeping, and proper exercise. If it is difficult to control stress and the cumulative fatigue caused by excessive work can not be resolved, it may be an option to apply cosmetics containing antioxidants regularly. Vitamin C and E coenzyme Q10 have been mentioned as antioxidants that can reduce free radicals. Recently, flourine has been attracting attention. 'Fullerene' is a carbon tunable consisting of 60 carbon rods. Unlike conventional antioxidants that try to neutralize free radicals, soccer ball-shaped fullerenes inhibit free radicals at high speeds. It also has the function of inhibiting the production of melanin caused by ultraviolet rays, thereby reducing skin pigmentation.

The vergene and fullerene may be included in an amount of 0.1 to 2.0 wt%, preferably 0.5 to 1.5 wt%, and more preferably 1.0 wt%, based on the total weight of the cosmetic composition. When the content of verogenin and fullerene is less than 0.1% by weight, the effect of stabilizing vitamin C and the like is insignificant. When the content exceeds 2.0% by weight, the effect is small and the stability of the formulation may be affected. The preferred amount of verghenin and fullerene is 1.0 wt% based on the total weight of the cosmetic composition.

In addition, the blend weight ratio of verghenin and fullerene may be 3: 7 to 7: 3, more preferably 5: 5. Except for the mixing weight ratio, the effect of the use of verrenine and fullerene is small.

The cosmetic composition of the present invention may contain vitamin C and / or a derivative thereof, and the derivative of vitamin C may include any conventional vitamin C derivative developed as a cosmetic raw material. For example, the vitamin C or derivatives thereof may be formulated with various salts of ascorbic acid, ascorbic acid (e.g., sodium ascorbate, magnesium ascorbate, sodium ascorbyl phosphate and ascorbyl magnesium phosphate) and esters of ascorbic acid Acetate, ascorbyl propionate, ascorbyl palmitate, and ascorbyl stearate).

As used herein, the term "stable" as used in reference to vitamin C or its derivatives means an action that inhibits oxidation of vitamin C or its derivatives by oxidation-inducing factors such as heat, light and oxygen.

On the other hand, since the cosmetic composition of the present invention contains vitamin C or a derivative thereof as an active ingredient, the cosmetic composition of the present invention can be represented by various conventional uses for vitamin C or a derivative thereof.

The vitamin C or derivative of vitamin C may be contained in an amount of 5 to 30% by weight, preferably 10% by weight, based on the total weight of the cosmetic composition.

In one embodiment, the cosmetic composition for skin protection according to the present invention comprises 5 to 30% by weight of a vitamin C or vitamin C derivative, 0.1 to 2.0% by weight of vergene and fullerene, 30% by weight of a humectant, 0.01 to 0.05% by weight of a metal sequestering agent, and a residual amount of a solvent.

The solvent may be purified water, ethanol or a mixture thereof.

The moisturizing agent includes all the polyols having a moisturizing effect used in the technical field, and is preferably selected from the group consisting of butylene glycol, glycerin, 1,2-hexanediol, Lt; / RTI > The moisturizing agent may be contained in an amount of 10 to 30% by weight based on the total weight of the cosmetic composition. If the amount is out of the above range, the stability of the formulation may be deteriorated. More preferably, when it contains both butylene glycol, glycerin and 1,2-hexanediol as a moisturizing agent, butylene glycol is present in an amount of 10 to 25% by weight based on the total weight of the cosmetic composition, glycerin is added to the total weight of the cosmetic composition 1.0 to 10.0% by weight based on the total weight of the cosmetic composition, and 1,2-hexanediol in an amount of 1.0 to 5.0% by weight based on the total weight of the cosmetic composition.

The metal sequestering agent may be any metal sequestering agent used in the technical field, preferably di-sodium dithiary (EDTA-2Na). The metal sequestering agent may be contained in an amount of 0.01 to 0.05% by weight based on the total weight of the cosmetic composition. If the metal sequestrant is contained in an amount of less than 0.01% by weight, the stability of the formulation is poor, and if the metal sequestrant is contained in an amount exceeding 0.05% by weight, skin irritation may occur.

The components included in the composition and cosmetic composition of the present invention include components commonly used in the composition other than the above components as an active ingredient and include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And a carrier.

The compositions of the present invention may be prepared in any of the formulations conventionally manufactured in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto. More particularly, when the composition of the present invention is used as a cosmetic composition, it can be used as a cosmetic composition such as a soft lotion (skin), a convergent lotion, a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, , Packs, sprays, or powders.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention is excellent in the stability of the formulation according to the temperature change by stabilizing the vitamin C or its derivative by verrenine and fullerene, and is excellent in temperature change and preservation of the potency with time.

Accordingly, the cosmetic composition of the present invention containing verghenin and vitamin C that effectively stabilizes vitamin C has a physiological activity, that is, a whitening effect (e.g., inhibition of tyrosinase activity), a wrinkle improving effect, and an antioxidant effect Etc.) can be effectively delivered to the skin. In addition, a composition for stabilizing vitamin C containing vergene and fullerene as an active ingredient can be applied to the skin together with the vitamin C preparation to contribute to stabilization of the unstable vitamin C preparation, thereby effectively exhibiting the physiological activity of vitamin C can do.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

The verghenin used in the preparation examples and examples was vergerman monohydrate (Sigma, B6776) manufactured by Sigma Co., and C60 was used as fullerene. Vitamin C used 100% pure ascorbic synthetic vitamin.

Experimental Example  One : With verrenine Fuller's  Vitamin C stabilization test according to mixing weight ratio

Vitamin C stabilization experiment was performed according to the weight ratio of verogenin and fullerene. In order to test the stabilization according to the mixing weight ratio except for the influence of the other components, vergene and fullerene were mixed in the following weight ratio and 1.0 wt% with respect to the total weight, and the remaining amount was prepared by adding purified water. The titer was measured at 4 캜 for the refrigerated condition and the titer was measured using liquid chromatography (HPLC) for the cyclic test, which varied from -4 캜 to 40 캜 for 12 hours.

Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Berrynin: fullerin (1.0%) 0: 10 1: 9 3: 7 5: 5 7: 3 9: 1 10: 0 cold storage 81% 84% 89.2% 99% 89.8% 83.5% 80% Room temperature 79% 80.5% 87% 95.2% 87.6% 80.8% 77% 45 ° C 65% 69.7% 71.5% 91.0% 73.5% 70.7% 66% Cyclic test 73% 75% 79.8% 93.4% 80.8% 74.7% 75%

As can be seen from the above Table 1, it can be seen that different effects are obtained depending on the mixing weight ratio of each component even if the same amount as the whole is used as the vitamin stabilizer. Excellent results can be obtained when verghenin and fullerene are mixed at a weight ratio of 3: 7 to 7: 3 (samples 3 to 5). When verulinin and fullerene are mixed at a weight ratio of 5: 5 (sample 4) The most excellent effect was obtained.

Experimental Example  2 : With verrenine Fuller's  Vitamin C by content Potency  Test

In order to confirm the effect of vitamin C stabilization on the content of verrenine and fullerin, a potency test was conducted. The experiment was carried out in the same manner as in Experimental Example 1.

Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 Sample 13 Verganin: Fullerin
(5: 5) 0.1% 0.3% 0.5% 1.0% 1.5% 2.0% cold storage 51% 64% 69.2% 99% 89.5% 83.3% Room temperature 59% 60.5% 67% 95.2% 87.6% 80.6% 45 ° C 35% 49.7% 61.5% 91.0% 73.7% 70.4% Cyclic test 43% 55% 65.8% 93.4% 80.4% 74.5%

As can be seen from the above Table 2, the effect was different depending on the content of verrenine and fullerin, and the most excellent effect was obtained when the content of verrenine and fullerene was 1.0 wt% (sample 11) .

Example  And Comparative example  Produce

Aqueous component compositions according to the present invention were prepared with the components and composition ratios shown in Table 3 below. Specifically, Examples and Comparative Examples were prepared by stirring at room temperature with AGI MIXER (MATSUSHITA ELECTRIC INDUSTRIAL CO.LTD) in the components and composition ratios shown in Table 3 below.

phrase
minute Main Category Raw material Composition ratio (% by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 One The first solvent Purified water to 100 to 100 to 100 to 100 to 100 2 The first solvent ethanol 8.0 8.0 8.0 8.0 8.0 3 Vitamins Vitamin C - 10 10 10 10 4 Metal blocker EDTA-2Na 0.02 0.02 0.02 0.02 0.02 5 Moisturizer 1.2-hexanediol 0.2 0.2 0.2 0.2 0.2 6 Moisturizer  Butylene glycol 15 15 15 15 15 7 Moisturizer glycerin 4 4 4 4 4 8 Vitamin stabilizer Bergenin - - 1.0 - 0.5 9 Vitamin stabilizer Fleurin - - - 1.0 0.5 total 100 100 100 100 100

Experimental Example  3: Experiment of discoloration according to temperature change

In order to test the stability of the cosmetic preparation prepared in the present invention, the degree of discoloration according to the temperature of Comparative Examples 1 to 4 and Example 1 was confirmed. In a thermostatic chamber maintained at 45 ° C constantly, the cells were stored in an opaque glass container for 1 week, and stored in a light-shielded refrigerator maintained at 4 ° C in an opaque glass container for 1 week. Respectively. The degree of discoloration of the product was classified into the following six grades.

0: No change, 1: Extremely slightly changed, 2: Slightly changed, 3:

4: Severely changed, 5: Extremely changed

Temperature Degree of discoloration of experimental material Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 45 ° C 0 5 4 4 2 4 ℃ 0 4 3 3 One

As can be seen from the above Table 4, the degree of discoloration in Example 1, which includes both verenin and fullerene, is relatively weak.

Experimental Example  4: Temperature change and time Potency  Conservation experiment

Comparative Examples 1 to 4 and Example 1 were stored in an opaque glass container in a thermostatic chamber maintained at 4 DEG C and 45 DEG C, respectively. After 12 weeks of storage, the remaining amount of ascorbic acid was determined using liquid chromatography (HPLC).

Treatment group 0 week / survival rate 1 week / remaining ratio 2 weeks / survival rate 4 weeks / survival rate Sample 1 4 ℃ - - - - 45 ° C - - - - Room temperature - - - - Sample 2 4 ℃ 100% 88.5% 50.6% 33.7% 45 ° C 100% 77.4% 35.5% 15.8% Room temperature 100% 79.5% 45.7% 30.7% Sample 3 4 ℃ 100% 91.5% 89.7% 79.4% 45 ° C 100% 89.7% 82.4% 77.6% Room temperature 100% 90.6% 88.5% 77.9% Sample 4 4 ℃ 100% 93.8% 90.6% 81.5% 45 ° C 100% 91.9% 90.1% 79.5% Room temperature 100% 95.7% 90.5% 80.9% Sample 5 4 ℃ 100% 99.5% 99.1% 98.9% 45 ° C 100% 99.1% 98.9% 98.5% Room temperature 100% 99% 98.9% 98.7%

As shown in Table 5, the content of vitamin C (ascorbic acid) in Example 1 containing vitamin C, vergene and fullerene hardly changed (residual ratio 98% or more), and after 4 weeks More than 98.5% of vitamin C (ascorbic acid) was detected.

Experimental Example  5: Free radical Scatters  exam

The free radical scavenging capacities of Comparative Examples 1 to 4 and Example 1 were measured as follows. Free radical scavenging activity was measured by a method using 1,1-diphenyl-2-picryl-hydrazine (DPPH) described in Nature magazine Blois, M. S., Nature (1958) vol. DPPH is a relatively stable free radical, exhibiting maximum absorption at 517 ㎚ in the presence of radicals, and loss of absorption when eliminated. DPPH was purchased from Sigma Chemical Company and dissolved in methyl alcohol at a concentration of 0.15 mM. First, Comparative Examples 1 to 4 and Example 1 were pre-treated with a potassium phosphate buffer solution, filtered with a 0.5 μm filter, and then prepared at a concentration of 1%, and 100 μl of each was added to each well of a 96-well plate. 100 μl of the DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes. Then, the absorbance was measured at 517 nm using a microplate reader (Biotek EL-340).

Treatment group Free radical scavenging ability (%) Average abatement (%) The first experiment Second experiment Third experiment Comparative Example 1 0% 0% 0% 0% Comparative Example 2 52.5% 50.9% 49.8% 51.06% Comparative Example 3 88.5% 88.7% 89% 88.7% Comparative Example 4 89% 88.5% 89.1% 89% Example 1 95.8% 95.8% 96.9% 96.1%

As can be seen from the above Table 6, in Comparative Example 2 containing only vitamin C, Comparative Example 3 and 4 containing verrenine and fullerene, Example 1 containing vitamin C and verrenine and fullerene Showed excellent free radical scavenging ability. This shows that vitamin C is stabilized by verrenine and fullerene, and the antioxidant capacity of stabilized vitamin C is also increased.

Experimental Example  6: Ultraviolet Human fibroblast  Test for inhibition of death

When ultraviolet rays are irradiated on fibroblasts, active oxygen is secreted in the cells and the cells are damaged. To induce the above phenomenon artificially, human fibroblasts (Korean Cell Line Bank) were inoculated into each well of a 48-well microplate at a density of 1 x 10 ⁴ cells, and cultured in DMEM medium (Sigma Co., ) For 24 hours at 37 < 0 > C. After irradiating with ultraviolet light (UVA), Example 1 was adjusted to concentration [0.1, 0.5, 1.0, 2.0 wt%] in each well and added to the culture solution. After 24 hours, cell activity was confirmed by MTT assay Respectively.

Example 1 (% by weight) Cell survival rate (%) Untreated cells UV irradiated cells 0.0 100 2.5 0.1 105.3 28.8 0.5 103.5 39.5 1.0 100.7 76.3 2.0 100.5 90.2

As shown in Table 7, Example 1 according to the present invention efficiently inhibited the death of fibroblasts by ultraviolet rays in a concentration-dependent manner.

Experimental Example  7: Buoyancy  Test test

A suspension of five bacteria ( S. aureus , E. coli , E. cloacae , P. aeruginosa , P. putida ) and three fungi ( C. albicans , A. niger, P. luteum ) were prepared in TST . 50 g of the cosmetic composition according to Example 1 was inoculated with 106-7 CFU / g and 105-106 CFU / g of bacteria and mold, respectively. The inoculated samples were stored at room temperature and taken 1 g each on days 7, 14, 21, and 28, and diluted to 1/1000 with dilution. Bacteria and fungi in the diluted samples were cultured in TSA medium and PDCA medium, respectively, and the mortality rate of the inoculated bacteria was determined.

The criterion is that the bacteria and fungus should be killed more than 99.9% and 95%, respectively, in the 7th day test, and the 14th day and subsequent tests shall be judged as appropriate if the microorganism does not proliferate.

The results according to the above criteria are shown in Table 8 below.

division After 7 days After 14 days After 21 days After 28 days Final result Example 1 fitness fitness fitness fitness fitness

Generally, it is common to use a preservative in cosmetic compositions, but as can be seen in Table 7, it can be seen that the cosmetic composition according to the present invention has a buoyant force even if the preservative is not included.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

And a derivative of vitamin C or vitamin C, in a cosmetic composition for protecting skin. The method according to claim 1,
The derivative of vitamin C is selected from the group consisting of ascorbic acid or sodium ascorbate, magnesium ascorbate, sodium ascorbyl phosphate, ascorbyl magnesium phosphate, ascorbyl acetate, ascorbyl propionate, ascorbyl palmitate and ascorbyl stearate ≪ / RTI > wherein the composition is selected from the group consisting of: The method according to claim 1,
Wherein the vergene and fullerene are contained in an amount of 0.1 wt% to 2.0 wt% based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the weight ratio of verene and furin is from 3: 7 to 7: 3. The method according to claim 1,
Wherein the weight ratio of vergene to fullerene is 5: 5. The method according to claim 1,
Wherein the vitamin C or derivative of vitamin C is contained in an amount of 5 to 30% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the cosmetic composition comprises 5 to 30% by weight of vitamin C or a derivative of vitamin C, 0.1 to 2.0% by weight of vergene and furin, 10 to 30% by weight of a moisturizer, 0.01 to 0.05% % Of a metal sequestering agent and a residual amount of a solvent. 8. The method according to any one of claims 1 to 7,
Wherein the vergene and fullerene stabilize the vitamin C or vitamin C derivative.