KR20170130307A - Cosmetic composition for whitening, antiaging or skin wrinkle containing natural oriental medicine extracts - Google Patents
Cosmetic composition for whitening, antiaging or skin wrinkle containing natural oriental medicine extracts Download PDFInfo
- Publication number
- KR20170130307A KR20170130307A KR1020170061184A KR20170061184A KR20170130307A KR 20170130307 A KR20170130307 A KR 20170130307A KR 1020170061184 A KR1020170061184 A KR 1020170061184A KR 20170061184 A KR20170061184 A KR 20170061184A KR 20170130307 A KR20170130307 A KR 20170130307A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- natural copper
- whitening
- cosmetic composition
- skin aging
- Prior art date
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/26—Iron; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/34—Copper; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The present invention relates to a composition comprising a natural copper extract or a fraction thereof as an active ingredient for whitening, preventing skin aging or improving skin wrinkles. More specifically, the present invention relates to a cosmetic composition, a pharmaceutical composition and a quasi-drug composition for whitening, preventing skin aging or wrinkles and containing the natural copper extract or a fraction thereof as an active ingredient, Skin aging prevention or wrinkle improvement method.
The natural copper extract according to the present invention is excellent in DPPH radical scavenging activity and is superior in cytotoxicity and protection effect against tyrosinase, elastase and UV-B irradiation, inhibits collagen fiber damage and inhibits melanin production And can be used for cosmetics, medicines and quasi-drugs for whitening, skin aging prevention, or wrinkle improvement.
Description
The present invention relates to a composition for improving whitening, preventing skin aging, or improving skin wrinkles containing natural herbal extract as an active ingredient. More specifically, the present invention relates to a cosmetic composition, a pharmaceutical composition and a quasi-drug composition for whitening, preventing skin aging or wrinkles and containing the natural copper extract or a fraction thereof as an active ingredient, Skin aging prevention or wrinkle improvement method.
Skin is the primary organ of aging, and aging of the skin is caused by various factors such as genetic factors, exposure to ultraviolet rays, various active oxygen species due to physical / chemical stress, and oxidative free radical generation, appear. Specifically, ultraviolet rays are divided into UV-A, UV-B and UV-C depending on the wavelength region. Among them, ultraviolet rays of UV-B wavelength cause pigmentation such as spots and black spots on the skin. In addition, ultraviolet rays form active oxygen in the skin cells, and various active oxygen thus generated damages intracellular proteins, lipids, nucleic acids, etc., decreases the amount of collagen synthesis, increases the amount of melanin synthesis, Matrix metalloproteinases (MMPs)), which break down the extra cellular matrix (ECM) such as collagen and elastin to cause various chronic skin diseases and skin aging including skin cancer and pigmentation. Accordingly, in recent years, there has been a need to develop a multifunctional functional material having various functions such as whitening, wrinkle improvement, skin aging improvement and ultraviolet ray shielding while protecting the skin from ultraviolet rays or active oxygen which is a main cause of skin aging.
As a result of research on cosmetics to prevent skin aging or wrinkles, cosmetic compositions for improving skin wrinkles containing the extract of Korean Horseradish Amphibia (Korean Patent Laid-Open Publication No. 2015-0143375), wrinkles containing chrysanthemum and chrysanthemum extract as active ingredients And a cosmetic composition for improvement (Korean Laid-Open Patent No. 2015-0009266).
However, in the case of using wrinkle-improving cosmetics, there is still a need to develop and research a cosmetic composition showing excellent wrinkle-improving or anti-aging effects, because it is 'unsatisfactory as expected' due to unsatisfactory cosmetics.
In addition, studies using natural copper, a mineral herb, a so-called mountain bone, reported treatment of a patient with delayed union of the fracture after femoral shaft fracture (J Korean Oriental Pediatrics, 25 (1): 63-71) (Japanese Patent Application No. 20-2003-0018484), the effect of natural copper on the osteoblastic activity of the human body and the bone fracture of the mouse sternum (The Journal of Korean Acupuncture & Moxibustion Society, vol. However, the antioxidative effect of natural antioxidants, skin whitening, skin wrinkles and skin aging are not yet known.
Under these circumstances, the inventors of the present invention have made intensive studies on a substance showing excellent skin improving effect from natural resources present in nature. As a result, it has been found that the natural copper extract has a tyrosinase inhibitory activity, an elastase inhibitory activity, a free radical scavenging activity, The present invention has been accomplished on the basis of confirming that it exhibits inhibition or protection of cell death by UV-B irradiation.
It is an object of the present invention to provide a cosmetic composition for whitening, preventing skin aging or improving wrinkles comprising a natural copper extract or a fraction thereof as an active ingredient.
Another object of the present invention is to provide a pharmaceutical composition for whitening, prevention of skin aging or wrinkles comprising a natural copper extract or a fraction thereof as an active ingredient.
Another object of the present invention is to provide a quasi-whitening composition for preventing whitening, skin aging, or wrinkles comprising a natural copper extract or a fraction thereof as an active ingredient.
Another object of the present invention is to provide a method for whitening, skin aging prevention or wrinkle improvement comprising the step of administering the composition to an individual other than a human.
The inventors of the present invention have found that natural copper extracts can inhibit tyrosinase inhibition activity, elastase inhibition activity, free radical scavenging activity, UV-B irradiation, and the like, as a result of various studies using materials showing excellent skin- Of human fibroblasts and human keratinocytes according to the present invention. Thus, it has been found that the natural copper extract can be used as a cosmetic composition for whitening, preventing skin aging, or improving wrinkles. The cosmetic composition containing the natural copper extract having the whitening effect, anti-aging effect of skin or wrinkle-improving effect has not been known at all, and it can be said that the cosmetic composition is very significant in that it was first developed by the present inventor.
In order to solve the above problems, there is provided, as one embodiment, a cosmetic composition for whitening, preventing skin aging or improving wrinkles comprising a natural copper extract or a fraction thereof as an active ingredient.
The term "pyritum ", as used herein, is also known as the mountain, and is well known as a mineral herb. The above-mentioned natural copper is mainly known for its effects on fractures and bones, but the inventors of the present invention first clarified the effect of natural whitening, skin aging prevention or wrinkle improvement. The natural copper may be purchased commercially, but is not limited thereto.
The term "extract " used in the present invention means a substance obtained by extracting a substance, specifically, an extract obtained by the natural copper extracting treatment of the present invention, a diluted solution of the extract, a concentrate, Extracts of all the formulations which can be formed using the extract itself and the extract, such as a dried product obtained by drying, a controlled preparation or a purified product of the above extract, or a mixture thereof.
The method of extracting the natural copper is not particularly limited, and extraction can be carried out according to a method commonly used in the art. Examples of the extraction method include a solvent extraction method, an ultrasonic extraction method, a filtration method and a reflux extraction method. These methods may be performed alone or in combination of two or more methods.
In the present invention, the kind of the extraction solvent used for extracting the natural copper is not particularly limited, and any solvent known in the art can be used. Nonlimiting examples of the extraction solvent include water, alcohols having 1 to 4 carbon atoms, and mixed solvents thereof. These solvents may be used alone or in combination. Specifically, the extraction solvent may be hydrothermal.
In one embodiment of the present invention, natural copper and mineral water were immersed in a Daewoong chemical bath and heated to obtain a natural copper extract, followed by lyophilization to prepare a natural copper extract sample.
The cosmetic composition of the present invention may contain the extract in an amount of 0.0001 to 10% by weight, and more preferably 0.0005 to 5% by weight, based on the whole composition.
If the content is less than 0.0001% by weight, the desired activity can not be sufficiently achieved, and if the content is more than 10% by weight, an increase in apparent effect is not expected to increase, which may be uneconomical.
As used herein, the term "fraction" refers to a result obtained by performing a fractionation to separate a particular component or group of specific components from a mixture comprising various components.
In the present invention, the fractionation method for obtaining the fraction is not particularly limited as long as it can exhibit antioxidant, whitening, anti-aging effect on skin or wrinkle of skin, but can be carried out according to a method commonly used in the art. For example, a solvent fractionation method performed by treating various solvents, an ultrafiltration fractionation method performed by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (separation by size, charge, hydrophobicity or affinity , And a combination thereof, and the like.
The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water, distilled water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohols are used in the fraction solvent, C 1 to C 4 alcohols can be preferably used.
In the present invention, the composition may be characterized by having an antioxidative activity.
The antioxidant means an oxidation inhibiting action. The human body has a balance of prooxidant and antioxidant. However, due to various factors, such a balance state is imbalanced, Tilting induces oxidative stress leading to potential cellular damage and pathological disease.
In one embodiment of the present invention, it was confirmed that the natural copper extract had an excellent DPPH free radical scavenging activity, so that the natural copper extract had an antioxidative activity (FIG. 3).
The term "whitening " as used in the present invention means whitening skin and means all actions that inhibit the synthesis of melanin and inhibit or prevent skin deposition of melanin.
In the present invention, the composition may be characterized by inhibiting the activity of tyrosinase.
The tyrosinase is a metal-containing protein containing a pair of copper ions in its active site. L-tyrosine is hydroxylated by 3,4-dihydroxyphenylalanine (L-DOPA) in the production pathway of melanin, , Which is then oxidized again with phenylalanine-3,4-quinone (dopaquinone), then enzymes involved in the step of synthesizing melanin via various intermediates such as dopachrome, indole-5,6-quinone .
In one embodiment of the present invention, it was confirmed that the natural copper extract had a tyrosinase inhibitory activity in a concentration-dependent manner, and thus the natural copper extract had a whitening activity (FIG. 1).
In the present invention, the composition may be characterized by inhibiting the production of melanin.
The melanin is a coloring matter of blackish brown granules, and is produced by a cell organelle called melanocytes in melanocytes. Melanin is present in skin, hair, eyes, and skin color is determined by the amount of melanin. The more melanin, the darker the skin color.
In one embodiment of the present invention, it was confirmed that the natural copper extract had a melanin production inhibitory effect in a concentration-dependent manner, and thus the natural copper extract had a whitening activity (FIG. 7).
As used herein, the term "wrinkles " refers to the scarring caused by degeneration of the skin, which can be caused by a cause of a gene, a decrease in collagen existing in the skin dermis, or an external environment.
In the present invention, the composition may inhibit the activity of elastase.
The elastase refers to an enzyme having a role of catalyzing the hydrolysis reaction of elastin involved in skin elasticity.
In one embodiment of the present invention, it was confirmed that the natural copper extract had an activity of inhibiting elastase in a concentration-dependent manner, so that the natural copper extract had a wrinkle-improving activity (FIG. 2).
In the present invention, the composition may be characterized by inhibiting collagen fiber damage.
The collagen fiber is a fibrous tissue composed of collagen and exists in connective tissues such as bone, cartilage, skin, and tendon. When the skin is exposed to ultraviolet rays, collagen fibers involved in skin elasticity are damaged and the skin photoaging proceeds.
In one embodiment of the present invention, it was confirmed that the natural copper extract had a wrinkle-reducing activity (Fig. 6).
As used herein, the term "skin aging" refers to symptoms such as skin elasticity reduction, gloss reduction, wrinkle formation, weakening of regenerative power, or severe drying, and may be caused by time or external environment.
In the present invention, the skin aging may be photoaging.
The photo-aging refers to a phenomenon in which skin elasticity and moisture are reduced and wrinkles are formed when skin is repeatedly exposed to ultraviolet rays of sunlight or long-term exposure, thereby causing skin damage.
In one embodiment of the present invention, it was confirmed that when the natural copper extract was added to human Hs27 cells and human HaCat cells, the survival rate of the cells was increased compared with the case of UV-B irradiation only after irradiation with UV-B, It was found that the extract had an effect of suppressing photoaging or protecting it from photoaging (FIG. 4).
As used herein, the term "improvement" refers to the use of the composition of the present invention to inhibit or inhibit the symptoms of melanin skin deposits, skin aging or wrinkles, or to alleviate symptoms of melanin skin deposits, skin aging or wrinkles It is said.
In the present invention, the cosmetic composition may be in the form of a solution, an external ointment, a cream, a foam, a nutritional lotion, a flexible lotion, a pack, a flexible water, a latex, a makeup base, an essence, It may be formulated as a suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, patch and spray It is not limited.
The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.
The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.
When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.
When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.
When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, But is not limited thereto. These may be used alone or in combination of two or more.
In another aspect, the present invention provides a pharmaceutical composition comprising a natural copper extract or a fraction thereof as an active ingredient for whitening, preventing skin aging, or improving wrinkles.
The terms "natural copper", "extract", "fraction", "whitening", "skin aging", "wrinkles", "improvement" as used herein are as described above.
The pharmaceutical composition of the present invention can be used as a single preparation and can be used as a combined preparation containing a drug known to have an effect of improving wrinkles or aging of the skin and can be formulated using a pharmaceutically acceptable carrier or excipient May be prepared in unit dosage form or may be manufactured by intrusion into a multi-dose container.
The term "pharmaceutically acceptable carrier" may mean a carrier, excipient or diluent which does not disturb the biological activity and properties of the compound to be injected, without irritating the organism. Specifically, occuring carrier. The type of the carrier that can be used in the present invention is not particularly limited, and any carrier conventionally used in the art and pharmaceutically acceptable may be used. Non-limiting examples of the carrier include saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and the like. These may be used alone or in combination of two or more.
The composition comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Particularly, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and these solid preparations may contain at least one excipient such as starch, calcium carbonate, sucrose, lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of liquid formulations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. Examples of the non-aqueous solution and suspension include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of the suppository base include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
The pharmaceutical composition may be administered in a pharmaceutically effective amount.
The "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dosage level will vary depending on the species and severity, age, sex, The time of administration, the route of administration and the rate of excretion, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts.
Another aspect of the present invention provides a quasi-product composition for whitening, preventing skin aging or improving wrinkles comprising a natural copper extract or a fraction thereof as an active ingredient.
The terms "natural copper", "extract", "fraction", "whitening", "skin aging", "wrinkles" used in the present invention are as described above.
The term "quasi-drug product" used in the present invention means products that are less active than drugs, among the products used for diagnosing, treating, improving, alleviating, treating or preventing diseases of human or animal. For example, Quasi-drugs are products that are used for the purpose of treating or preventing diseases of humans or animals, products that are mild or have no direct action on the human body.
The quasi-drug composition of the present invention can be manufactured by, but not limited to, a body cleanser, a foam, a soap, a mask, an ointment, a cream, a lotion, an essence and a spray.
When the composition according to the present invention is used as a quasi-drug additive, the composition may be added as it is or may be used together with other quasi-drugs or quasi-drugs, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.
In another embodiment, the present invention provides a method for whitening, preventing skin aging or wrinkles, comprising the step of administering to a subject a composition comprising a natural copper extract or a fraction thereof.
The terms "natural copper", "extract", "fraction", "whitening", "skin aging", "wrinkles" used in the present invention are as described above.
The term "administering" as used herein means introducing the composition of the present invention to a patient in any suitable manner, and the administration route of the composition can be administered through any conventional route so long as it can reach the target tissue . But are not limited to, intraperitoneal, intravenous, intramuscular, subcutaneous, intradermal, oral, topical, intranasal administration. Due to the fact that the composition of the present invention is effective for antioxidant, whitening, skin aging or wrinkle improvement, the administration route of the composition can be applied to the skin and administered.
The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or two or three times. In addition, the composition of the present invention can be used alone or in combination with other drug treatments to improve antioxidant, whitening, skin aging or wrinkles. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art.
As used herein, the term "individual" refers to all animals such as mice, mice, livestock and the like, including humans who have developed or are capable of developing wrinkles or skin aging. Specifically, it may be a mammal including a human.
The natural copper extract according to the present invention is excellent in DPPH radical scavenging activity and is superior in cytotoxicity and protection effect against tyrosinase, elastase and UV-B irradiation, inhibits collagen fiber damage and inhibits melanin production And can be used for cosmetics, medicines and quasi-drugs for whitening, skin aging prevention, or wrinkle improvement.
1 is a graph showing tyrosinase inhibitory activity according to the concentration of the natural copper extract supernatant.
2 is a graph showing the inhibitory activity of elastase according to the concentration of natural copper extract.
3 is a graph showing DPPH free radical scavenging activity according to natural copper extract treatment.
4 is a graph showing the cell survival rate of human fibroblast Hs27 cells according to the concentration of natural copper extract.
FIG. 5 is a graph showing the cell survival rate of HsCat cells, which are human keratinocytes, according to the concentration of natural copper extract.
6 is a graph showing collagen damage inhibitory activity of artificial skin according to the concentration of natural copper extract.
7 is a graph showing the activity of inhibiting melanin formation of artificial skin depending on the concentration of natural copper extract.
Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these embodiments are intended to illustrate the present invention, and the scope of the present invention is not limited to these embodiments.
Example 1. Preparation of Natural Copper Extract
In order to prepare natural copper extracts, hot water extraction was carried out by the following method.
Specifically, 50 g of natural copper was placed in a Daewoong water tank (DWP-5000M) together with 1 L of bottled water (White, Co., Ltd., Murakami Sanseong Spring, Kyungnam Sancheong-gun) and immersed for 1 hour and then heated for 3 hours to obtain natural copper extract . The natural copper extract was filtered through a filter sieve (Test sieve 106 μm (No. 140), Retsch, Germany), frozen in a cryogenic freezer and lyophilized to prepare a natural copper extract sample.
Example 2. Measurement of whitening activity of natural copper extracts
In order to measure the whitening activity of the natural copper extract, the tyrosinase inhibitory activity of the natural copper extract was confirmed.
Specifically, the extract was diluted with PBS to a final concentration of 0.5, 1.25, 2.5, 5, and 12.5% by weight of the natural copper extract, centrifuged at 3000 rpm for 5 minutes, and the resulting supernatant was taken. 50 μl of the supernatant was mixed with 10 μl of 0.2 M sodium phosphate buffer (pH 6.8), 50 μl of 10 mM L-tyrosine and 40 μl of mushroom tyrosinase (1000 U / ml) Min. ≪ / RTI > After the reaction, the absorbance at 510 nm was measured using an ELISA reader, and the tyrosinase inhibitory activity (%) was calculated using the following equation:
Tyrosinase inhibitory activity (%) = 1- (absorbance of sample addition group / absorbance of no addition group)
At this time, PBS was used instead of the natural copper extract supernatant, and ascorbic acid, which is well known for whitening activity, was used as a positive control.
Natural copper extract supernatant
As a result of the experiment, it was confirmed that the tyrosinase inhibitory activity of the natural copper extract supernatant was increased in a concentration-dependent manner (Fig. 1). In particular, it was confirmed that when the concentration of the supernatant of natural copper extract was 5 / / mL or more, it showed a high tyrosinase inhibitory activity of 90% or more similar to the positive control (Fig. 1 and Table 1).
Therefore, it was found that the natural copper extract has a whitening activity through the tyrosinase inhibitory activity of the natural copper extract.
Example 3. Measurement of wrinkle-improving activity of natural copper extract
In order to measure the wrinkle improving activity of the natural copper extract, the elastase inhibitory activity of the natural copper extract was confirmed.
Specifically, the final concentration of the natural copper extract was diluted to 0.4, 4, 20 and 40% by weight using a 0.2 M Tris-Cl solution (pH 8.0). 100 μl of the natural copper extract diluted to various concentrations was mixed with 100 μl of the substrate solution and 50 μl of the enzyme solution, followed by reaction at 37 ° C for 30 minutes. At this time, the substrate solution was added to 1 mL of 50 Mm Tris-Cl solution (pH 8.6), and 50 mM N-succinyl- (Ala) 3-p-Nitroanilide, which is an elastase substrate, (Ala) 3-pnitroanilide). In addition, the enzyme solution was diluted to a concentration of 0.6 units / mL of porcine pancreatic elastase (PPE) using a 50 mM Tris-Cl solution (pH 8.6).
Next, the reactants were measured for absorbance at 410 nm using an ELISA reader, and the inhibitory activity (%) of elastase was calculated using the following equation:
Elastase inhibitory activity (%) = {1- (absorbance of sample addition group / absorbance of no addition group)}
At this time, distilled water was used instead of the natural copper extract, and adenosine was used as a positive control group.
As a result of the experiment, it was confirmed that the activity of inhibiting elastase of natural copper extract was increased in a concentration dependent manner. In particular, it was confirmed that when the concentration of the natural copper extract was 40 ㎍ / mL or more, it exhibited a high inhibitory activity of elastase of 90% or more similar to the positive control (Fig. 2).
Therefore, it has been found that the natural copper extract has wrinkle improving activity through the inhibition of elastase of natural copper extract.
Example 4. Antioxidant activity measurement of natural copper extract
In order to measure the antioxidative activity of natural copper extracts, the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) Respectively.
Specifically, methanol was used to dilute the natural copper extract to a concentration of 100 / / mL. 150 [mu] l of the diluted natural copper extract was dispensed into 96 well plates with 150 [mu] l of DPPH solution dissolved in 0.4 mM using methanol, and reacted at room temperature for 30 minutes. Next, the reactants were measured for absorbance at 517 nm using an ELISA reader, and the DPPH free radical scavenging activity (%) was calculated using the following equation:
DPPH free radical scavenging activity (%) = {1- (absorbance of sample addition group / absorbance of no addition group)} x 100
At this time, distilled water was used instead of natural copper extract, and ascorbic acid was used as a positive control group.
Experimental results showed that DPPH free radical scavenging activity was enhanced when the natural copper extract was added. Specifically, it was confirmed that the natural copper extract-added group at a concentration of 100 ug / mL showed a DPPH free radical scavenging activity of 80% or more similar to the positive control group (FIG. 3).
Therefore, it was found that the natural copper extract has antioxidative activity through the high DPPH free radical scavenging activity of the natural copper extract.
Example 5. Natural copper extract Photoaging Suppression or protection effect
In order to measure the photoaging inhibition or protective effect of the natural copper extract, the inhibition or protective effect of the natural copper extract on cell death induced by UV-B irradiation was confirmed.
Specifically, human fibroblasts in Hs27 and human skin keratinocytes in HaCat cells, 5% CO 2, 37 ℃ and in keeping incubator for at least 95
As a result, the cell viability of human Hs27 cells was reduced to 100% or less when UV-B alone was irradiated, but the cell survival rate was increased to 100% or more when UV-B was irradiated after treatment with natural copper extract (Fig. 4). In addition, when human HaCaT cells were irradiated with UV-B alone, the cell viability was reduced to 100% or less, whereas when the natural copper extract was treated with UV-B, the cell viability was increased in a concentration-dependent manner 5).
Therefore, it was found that the natural copper extract exhibits photoaging inhibition or protection effect because the natural copper extract increases the cell survival rate of human Hs27 and HaCat cells irradiated with UV-B.
Example 6. Measurement of anti-wrinkle activity of artificial skin of natural copper extract
The wrinkle inhibitory activity of the natural copper extract was evaluated in 3D human skin. Specifically, the natural copper hydrothermal extract was treated with artificial skin for 1 hour and then irradiated with UVB at 50 mJ / cm 2 so as to have a final concentration of 50, 100% by weight. After incubation for 2 days in an incubator maintained at 5% CO 2 , 37 ° C and 95% humidity, the artificial skin was cut and fixed in 4% paraformaldehyde and processed into a paraffin block through tissue processing . The paraffin block was cut to a thickness of 5 μm and attached to the slide, followed by staining with masson trichrome stain. The wrinkle inhibiting activity was evaluated through blue stained collagen fibers through tissue staining. As a control group, adenosine and ascorbic acid were used.
As a result, collagen fibers were significantly decreased in UV-B irradiated artificial skin, whereas collagen fibers increased in UV-B irradiated after natural copper extract treatment. Especially, 100 μg / mL of natural copper In the treatment group, a level of collagen fibers similar to that of the control group not irradiated with UV-B and the positive control group treated with
Therefore, it was found that the natural copper extract exhibits wrinkle-inhibiting activity because the natural copper extract increases the amount of collagen fibers in the artificial skin irradiated with UV-B.
Example 7. Measurement of whitening activity of artificial skin of natural copper extract
The whitening effect of natural copper extracts was evaluated in 3D human skin. Specifically, the natural copper hydrothermal extract was treated with artificial skin containing melanin at a final concentration of 50, 100% by weight, cultured in an incubator maintained at a humidity of 5% CO 2 , 37 ° C and 95% And observed with a microscope to confirm the inhibition of melanin production. As a control group, ascorbic acid was used.
As a result, it was confirmed that melanin was decreased in the natural copper extract-treated group compared with the negative control group, and melanin was decreased in the
Therefore, it was found that the natural copper extract showed the whitening activity because the natural copper extract reduces the melanin production of the artificial skin.
It is to be understood that both the foregoing general description and the following detailed description of the present invention are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. More variations are possible within a range that does not. Accordingly, the present invention may be embodied in other ways than those specifically described and illustrated herein, and it may be understood by those skilled in the art.
Claims (12)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20160060288 | 2016-05-17 | ||
| KR1020160060288 | 2016-05-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20170130307A true KR20170130307A (en) | 2017-11-28 |
| KR101908077B1 KR101908077B1 (en) | 2018-10-16 |
Family
ID=60325247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020170061184A KR101908077B1 (en) | 2016-05-17 | 2017-05-17 | Cosmetic composition for whitening, antiaging or skin wrinkle containing natural oriental medicine extracts |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR101908077B1 (en) |
| WO (1) | WO2017200288A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102543697B1 (en) * | 2022-05-25 | 2023-06-15 | 주식회사 온톨로진바이오 | Cosmetic composition for improving atopic dermatitis containing hexa-peptide from yareta and manufacturing methods thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190125685A (en) * | 2018-04-30 | 2019-11-07 | 주식회사 단정바이오 | Aspergillus/Sorghum Bicolor Leaf/Stem fermented filtrate or fermented filtrate extract contained cosmetic composition for a skin whitening and anti-wrinkle and its preparation method |
| CN112870132A (en) * | 2021-04-07 | 2021-06-01 | 福建宸润生物科技有限公司 | Freckle-removing and whitening mask liquid and mask containing same |
| KR102539209B1 (en) | 2022-11-22 | 2023-06-02 | (주)엔비바이오컴퍼니 | Cosmetic composition for skin-whitening, anti-oxidant, anti-inflammatory, skin anti-irritation and moisturizing comprising the extract of Nymphaea alba, Backhousia citriodora and Malus pumila |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040041235A (en) * | 2002-11-09 | 2004-05-17 | 김한승 | Pharmaceutical composition comprising the water-soluble component of kupferkies and the extract of crude drug effective to treat neuronal paralysis and neuralgia |
| KR20050050321A (en) * | 2003-11-25 | 2005-05-31 | 이태후 | The compound for stimulating hair growth |
| KR20120015072A (en) * | 2010-08-11 | 2012-02-21 | 정진남 | Method for detoxifying of pyritum for dosing |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1097327A (en) * | 1994-01-26 | 1995-01-18 | 史建华 | Lotion for dry skin tinea and preparation method thereof |
| EP0759290B1 (en) * | 1995-08-14 | 1997-11-12 | GEOMEDICAL S.r.l. | Topical aqueous gels or suspensions based on pulverized volcanic or diagenetic minerals |
| JP2009167106A (en) * | 2008-01-10 | 2009-07-30 | Purunaito:Kk | Cosmetic |
-
2017
- 2017-05-17 WO PCT/KR2017/005102 patent/WO2017200288A1/en active Application Filing
- 2017-05-17 KR KR1020170061184A patent/KR101908077B1/en active IP Right Grant
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040041235A (en) * | 2002-11-09 | 2004-05-17 | 김한승 | Pharmaceutical composition comprising the water-soluble component of kupferkies and the extract of crude drug effective to treat neuronal paralysis and neuralgia |
| KR20050050321A (en) * | 2003-11-25 | 2005-05-31 | 이태후 | The compound for stimulating hair growth |
| KR20120015072A (en) * | 2010-08-11 | 2012-02-21 | 정진남 | Method for detoxifying of pyritum for dosing |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102543697B1 (en) * | 2022-05-25 | 2023-06-15 | 주식회사 온톨로진바이오 | Cosmetic composition for improving atopic dermatitis containing hexa-peptide from yareta and manufacturing methods thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101908077B1 (en) | 2018-10-16 |
| WO2017200288A1 (en) | 2017-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101908077B1 (en) | Cosmetic composition for whitening, antiaging or skin wrinkle containing natural oriental medicine extracts | |
| KR102047234B1 (en) | Composition for Improving Skin Conditions with Improved Skin Whitening, Antioxidant, Moisturizing, Anti-wrinkling, and Regeneration Property Comprising Callus Extract of Hibiscus Syriacus | |
| KR20110108687A (en) | Skin whitening agent containing extracts of natural materials and composition of cosmetic | |
| KR100715485B1 (en) | Cosmetic composition comprising the root extract of nymphaea tetragona having skin whitening and anti-aging activity | |
| KR20140006139A (en) | Cosmetic composition with anti-uv and anti-wrinkle effect from caragana sinica rehder | |
| KR20130072706A (en) | Method for preparing paeonia lactiflora extracts containing taxifolin-3-glucoside and cosmetic composition containing preparing paeonia lactiflora extracts | |
| KR100602684B1 (en) | Cosmetic composition comprising extract of erigeron breviscapus for whitening | |
| KR101440675B1 (en) | Composition for Improving Skin Wrinkle Comprising Ginseng Oil as Active Ingredient | |
| KR102002997B1 (en) | Cosmetic composition including taxifolin glucosides and extracting method of paeonia lactiflora extracts | |
| KR102226179B1 (en) | Cosmetic Compositions for Anti-aging Comprising Extracts of Plants | |
| KR102154927B1 (en) | A cosmetic composition for anti-aging, anti-oxidant, skin regeneration comprising chlorogenic acid, ferulic acid, resveratrol, and Streptococcus thermophilus fermented extract | |
| KR100865071B1 (en) | Composition comprising the saururus chinensis extract or compound isolated therefrom for skin whitening | |
| KR20080096162A (en) | Cosmetic composition for whitening skin | |
| KR20130022476A (en) | A cosmetic composition containing extract of artemisia iwayomogi | |
| KR101243220B1 (en) | Skin whitening and anti-wrinkle composition comprising a fermented plant extract in Ulleung island | |
| KR20140081137A (en) | A skin-care agent containig Morchella esculenta fruit body extract or Morchella esculenta mycelium extract | |
| KR102054132B1 (en) | Composition comprising extract of Prasiola japonica for skin whitening | |
| KR101317433B1 (en) | The cosmetic composition for skin whitening comprising the mixture of extract of Hovenia dulcia, Platycodon grandiflorum, wheat bud and sage | |
| KR20120118946A (en) | Skin whitening complex containing trihydroxyisoflavone and glycyrrhiza uralensis extracts | |
| KR101176525B1 (en) | Cosmetic Composition Comprising Acer tegmentosum Maxim as Active Ingredient | |
| KR102636105B1 (en) | Cosmetic composition including extract of rhododendron brachycarpum or extract of rhododendron fortunei | |
| KR102435602B1 (en) | Cosmetic composition for skin improvement containing aloe vera adventitious root extract | |
| KR20190015683A (en) | Composition for improving skin conditions comprising Dendropanax Morbifera extracts-metal nanoparticles complex | |
| KR100561781B1 (en) | Composition for skin whitening containing extract of Sinomenium acutum | |
| KR102186533B1 (en) | A cosmetic composition for skin whitening comprising Rosa Damascena Flower Oil and Rhodiola Rosea root extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |