KR20170116432A - Soap composition comprising red ginseng for improving atopic dermatitis and preparation method thereof - Google Patents

Abstract

The present invention relates to a red ginseng soap composition for improving atopic dermatitis comprising red ginseng fermentation powder, bokbunja extract, and screened fermentation extract, and a method for preparing red ginseng soap for improving atopic dermatitis.

Description

TECHNICAL FIELD The present invention relates to a red ginseng soap composition for improving atopic dermatitis and a method for preparing the red ginseng composition for red ginseng for improving atopic dermatitis,

The present invention relates to a red ginseng soap composition for improving atopic dermatitis and a method for producing the same.

As the environment becomes increasingly polluted, skin diseases that are unknown cause, such as atopic dermatitis (atopic dermatitis) are rapidly increasing. It usually starts in infancy or childhood and grows for a long period of time or recurrence. Skin dryness, eczema, skin inflammation, erythema and itching are present. These atopic dermatitis use moisturizers or, in severe cases, antihistamines and steroid ointments to provide adequate hydration to dry skin and reduce itching and dermatitis. However, prolonged use of antihistamines and steroid ointments may result in tolerance or irritation of the skin, resulting in irritation, irritation, swelling and acne, which can lead to damage to the immune system during prolonged use.

Various functional soaps have been introduced to prevent or ameliorate such atopic dermatitis and various skin diseases. However, the conventional soap maximizes the detergency and removes the oil of the skin unnecessarily in the washing process, thereby making the skin sensitive and dry, and the skin improving action such as moisturizing, antibacterial and anti-inflammatory action is not effectively applied.

In order to solve the above-mentioned problems, the present invention is to produce soaps using by-products of red ginseng, which is in the spotlight for immunosuppression, anti-inflammatory action, blood flow circulation improvement and vasodilatory action promotion.

Korean red ginseng is ginseng (panax ginseng) which is processed by repeating the process of fermentation with steam, drying and ripening without peeling the skin. Red ginseng contains 30 kinds of saponin which is a physiologically active substance and 14 kinds of ginsenosides such as ginsenoside Rb ₁ , Rb ₂ , Rh ₁ , Rh ₂ , Rg ₂ , Rg ₃ , Rg ₅ and Rf It is known that a unique ingredient shows excellent pharmacological effect. In addition to saponin, red ginseng contains physiologically active substances such as polysaccharides, carbohydrates, nitrogen compounds, polyacetylenes, phenolic acids and vitamins.

Particularly, polysaccharides are mainly composed of glucose, arabinose, galactose, xylose and rhamnose, and it is known that anti-complementary activity is active. Anti - complement activity stimulates immune cells and activates external infection defense. It is also known to have excellent anti-cancer effect.

Red ginseng can be obtained by conventional extraction using water or alcohol as a solvent. However, the red ginseng residue, which is a byproduct remaining after the extraction process, contains 20 to 40% of the above-mentioned physiologically active substances, but it is not recovered and is abandoned. In addition, since the red ginseng starch also increases the viscosity of the concentrate, it is in the process of being refined after the extraction of red ginseng.

Korean Registered Patent No. 10-0497665 (Jun. 17, 2005)

In order to solve the above problems, red ginseng fermentation powder having increased yield of polysaccharide and red ginseng byproduct is prepared by reacting abandoned red ginseng by-product with coenzyme. It is an object of the present invention to provide a red ginseng soap composition capable of improving the itching and dermatitis caused by atopic dermatitis by mixing the red ginseng fermentation powder with the bokbunja extract and the screen fermentation extract and aging the mixture.

In addition, the present invention relates to a red ginseng soap composition for improving atopic dermatitis, which is excellent in skin moisturizing, antibacterial and anti-inflammatory properties, and is used in combination with aloe vera leaf extract, bokbunja extract, propolis, honey, shea butter, coconut milk, And a method for producing the same.

In order to accomplish the above object, the present invention relates to a red ginseng soap composition for improving atopic dermatitis comprising red ginseng fermented powder, bokbunja extract, and screened fermented extract.

The present invention also provides a method for preparing a red ginseng fermented product, comprising the steps of: (a) adding water and a coenzyme to a red ginseng by-product, reacting the mixture at 40 to 60 ° C for 30 minutes to 30 hours, (C) a fermented red ginseng powder obtained in the step (a); and (b) a step of reacting the fermented red ginseng powder obtained in the step (a) (D) adding a soap base, a moisturizing agent and water to a stirrer, heating and emulsifying the mixture, and (c) stirring the mixture at a temperature of 30 to 50 ° C for 5 to 20 hours. And then injecting and coagulating the mixture into a soap bar. The present invention also relates to a method for preparing red ginseng soap for improving atopic dermatitis.

The red ginseng soap composition for improving atopic dermatitis according to the present invention is prepared by using coenzyme to produce red ginseng fermented powder having increased yield and polysaccharide content of red ginseng to increase the immunity of skin and to improve atopic dermatitis As shown in FIG. Since the red ginseng fermentation powder is mixed with the bokbunja extract and the screen fermentation extract and aged, it is possible to produce a soap which is effective in alleviating skin irritation, wound healing, itching and dermatitis relief due to atopic dermatitis.

In addition, it is possible to make the skin vigorous by producing soap containing red ginseng powder, aloe vera leaf extract, propolis, honey, shea butter, coconut milk and natural oil, Antibacterial and antioxidant effect gives skin elasticity and prevents skin aging. The soap produced in the present invention can be used as a shampoo as well as an eyewash and a body shampoo.

1 is a graph showing the RAW 264.7 cell viability of red ginseng fermentation powder according to an embodiment of the present invention,
2 is a graph showing NO production in RAW 264.7 cells of red ginseng fermentation powder according to an embodiment of the present invention,
Figure 3 is a graph showing TNF-a production in RAW 264.7 cells of red ginseng fermentation powder according to an embodiment of the present invention.

Hereinafter, the red ginseng soap composition for improving atopic dermatitis according to the present invention and its preparation method will be described in detail.

The present invention relates to a red ginseng soap composition for improving atopic dermatitis, which comprises red ginseng fermentation powder, bokbunja extract, and folded fermentation extract.

Specifically, the present invention uses fermented red ginseng to produce fermented red ginseng powder with increased yield and polysaccharide content of red ginseng. Therefore, it increases the immunity of skin by enhancing the anticoagulant activity of polysaccharide and promotes blood circulation of skin , And there is a technical feature that can improve atopic dermatitis through antibacterial and anti-inflammatory action.

Specifically, in the present invention, a screen-fungus fermented extract is mixed with Lactobacillus plantarum plantarum , Lactobacillus casei and Streptococcus lactis to reduce the irritation of atopic dermatitis, to exhibit anti-inflammatory effect and excellent wound healing power, There is a technical feature that maximizes the effect of the extract.

In addition, since the soap composition is prepared by aging the mixture of the red ginseng fermentation powder, the bokbunja extract and the screen fermentation extract, the anti-inflammation and antimicrobial properties are excellent, which is effective for improving atopy and is also effective for wound healing.

In addition, by producing soaps further containing red ginseng powder, aloe vera leaf extract, propolis, honey, shea butter, coconut milk and natural oil, the irritation feeling is small when using soap, and the skin is rich in moisture and nutrition, And a red ginseng soap for improving atopic dermatitis which can make the skin surface smooth and smooth.

The term " red ginseng roots " in the present invention refers to residues of red ginseng extracted with a solvent such as water or alcohol. The red ginseng contains saponin as well as physiologically active substances such as acid polysaccharide, carbohydrate, nitrogen compound, polyacetylene, phenolic acid, vitamin and protein which are not soluble in water.

In the present invention, the term 'red ginseng starch' refers to a carbohydrate mainly contained in red ginseng roots. Red ginseng starch is a substance obtained by extracting red ginseng with water or ethanol and leaving the remaining residue in a dry state without adding any residue.

The term " red ginseng byproduct " in the present invention may include, but is not limited to, any one selected from the above red ginseng roots and red ginseng starch, or a mixture thereof.

In the present invention, the term 'coenzyme' usually means a commercially available enzyme or coenzyme obtained from malt, but preferably means coenzyme obtained from malt.

The term " red ginseng fermented powder " in the present invention is obtained by reacting a red ginseng byproduct with a coenzyme obtained from malt, and a red ginseng by-product obtained by enzymatic treatment.

In the present invention, the term " screening fermented extract" means a strain containing at least one selected from a screening-leaf extract, Lactobacillus plantarum , Lactobacillus casei and Streptococcus lactis ≪ / RTI >

In the present invention, the red ginseng fermentation powder is obtained by reacting the red ginseng byproduct with the coenzyme obtained from malt, and the red ginseng byproduct is obtained by enzymatic treatment. Specifically, red ginseng fermentation powder prepared by reacting a coenzyme with a by-product of red ginseng, and then drying the supernatant obtained by centrifuging can be used.

Specifically, the coenzyme can usually be a commercially available enzyme or coenzyme obtained from malt, but preferably using a coenzyme obtained from malt may further increase the polysaccharide yield of the red ginseng fermentation powder, And therefore it is preferable.

The malt is rich in amylase, a starch hydrolyzing enzyme, and contains more than 30 kinds of enzymes. In addition, it contains lecithin and vitamin B, vitamin C, potassium, calcium and various minerals that can maintain the moisture of the horny layer of the skin, which can help improve atopic dermatitis.

More specifically, the coenzyme is preferably diluted with water to prepare the concentrate at a concentration of 0.01 to 3% by weight, more preferably 0.1 to 1% by weight, to increase the polysaccharide yield of the red ginseng by-product Do.

In the present invention, it is preferable to prepare a soap composition including a mixture containing red ginseng fermentation powder, bokbunja extract, and screened fermentation extract.

The red ginseng fermentation powder is preferably used in an amount of 60 to 80% by weight, more preferably 65 to 75% by weight, of the mixture. It is possible to effectively work to improve atopic dermatitis within the above-mentioned range, and the surface of the soap is not roughened.

In the present invention, the bokbunja extract contains a large amount of antioxidant components such as vitamin, polyphenol, anthocyanin, keratin and vitamin B and C, and is excellent in antioxidative effect, thereby preventing skin aging and maintaining skin elasticity. The solids content of the extract may be from 0.1 to 5% by weight, but is not limited thereto.

Specifically, the bokbunja extract preferably contains 1 to 10% by weight, more preferably 2 to 7% by weight, of the mixture. The antioxidant effect is enhanced within the above range and the viscosity is not affected during the production of the soap, which is preferable

In the present invention, the screening leaves extract contains Asiatic acid, Madecassic Acid, madecassoside and Asiaticoside, and is useful for treating skin inflammation, It is an effective substance for healing wounds due to atopic dermatitis and improving dermatitis because it strengthens barrier as well as skin calming. The solids content of the extract may be from 0.1 to 5% by weight, but is not limited thereto.

The folded-leaf extract was obtained from Lactobacillus plantarum plantarum , Lactobacillus casei and Streptococcus lactis may be used as the screen-fermented extract obtained by reacting with a strain containing at least one selected from the group consisting of Lactobacillus plantarum , Lactobacillus casei and Streptococcus lactis .

Specifically, it is preferable that the folded-leaf-type fermented extract be mixed with the screenflow extract: strain at a weight ratio of 1: 9 to 9: 1. It is possible to obtain a screen-fed fermented extract in which the skin irritation and wound healing effects by atopic dermatitis are further increased within the above range.

Specifically, it is preferable to use the screen-fermented fermentation extract in an amount of 10 to 30% by weight, preferably 15 to 25% by weight, in the mixture. It is possible to produce a soap that is well-compatible with the ingredients of the soap composition within the above-mentioned range, and is effective in improving atopic dermatitis, such as skin recovery effect and anti-inflammatory action.

The soap base in the present invention includes higher fatty acids and basic solutions of C ₆ to C ₂₂ .

Specifically, the C ₆ to C ₂₂ higher fatty acids are selected from the group consisting of Caproic Acid, Heptanoic Acid, Caprylic Acid, Pelargonic Acid, Capric Acid, Undecanoic Acid, Lauric Acid, Tridecanoic Acid, Myristic Acid, Pentadecanoic Acid, Palmitic Acid, Myristic Acid, Margaric Acid, Stearic Acid, Arachidic Acid, Behenic Acid, and the like may be used.

More specifically, in the present invention, when sodium laurate, sodium stearate and sodium myristate in salt form in the higher fatty acid are mixed together, they are rich in foam, This excellent soap can be produced.

Specifically, the basic solution may be one or more selected from sodium hydroxide (NaOH), potassium hydroxide (KOH), triethanolamine (TEA) and the like, preferably triethanolamine (TEA) .

The soap base may further comprise a surfactant.

The surfactant may be selected from sodium laureth sulfate, sodium alkylbenzenesulfonate and sodium monoalkylphosphate, but preferably sodium laurate sulfate is used. Is not irritating to the skin, and environmental pollution can be reduced.

Specifically, the soap base comprises 10 to 30% by weight of sodium laurate, 15 to 30% by weight of sodium stearate, 10 to 30% by weight of sodium myristate, 4 to 15% by weight of sodium laureth sulfate and 12 to 30% It can be used including%. It is possible to produce a soap base having an excellent detergency and a rich foam in the above range.

In the present invention, it is preferable that 2 to 30 parts by weight of the mixture containing red ginseng fermentation powder, brambone extract, and screened fermentation extract is contained in 100 parts by weight of the soap base, and 5 to 25 parts by weight is effective for improving atopic dermatitis It may be preferable.

In the present invention, a moisturizer is a component capable of penetrating moisture into the stratum corneum of the skin and forming an oil film on the surface of the skin to prevent evaporation of water on the skin surface.

Specifically, the moisturizing agent is selected from the group consisting of glycerin, sorbitol, propylene glycol, butylene glycol, polyethylene glycol, ceramide, and sodium hyaluronate. Any one or a mixture of two or more selected may be used. Preferably, glycerin and sorbitol are mixed and used. Glycerin and Sorbitol can attract moisture to the skin, which helps maintain moisturizing, has no side effects on the skin, and can be easily dissolved to give a smooth feel.

Specifically, the humectant may be used in an amount of 10 to 40 parts by weight, preferably 15 to 25 parts by weight, based on 100 parts by weight of the soap base. Within the above range, the viscosity of the soap composition can be maintained stably during the production of the soap, and the skin can be kept moist.

More specifically, the moisturizing agent is preferably used in a weight ratio of glycerin: sorbitol of 4: 6 to 8: 2, more preferably of 6: 4 to 7: 3 of glycerin: sorbitol. The moisturizing action of the skin can be effectively performed within the above-mentioned range.

In the present invention, the red ginseng powder can be prepared by drying the red ginseng by-product and pulverizing the red ginseng powder to 80-100 mesh.

Specifically, it is preferable to use 0.1 to 15 parts by weight, more preferably 1 to 10 parts by weight, based on 100 parts by weight of the soap base. Within the above range, the physiologically active substances contained in the red ginseng by-products are effective for improving atopic dermatitis, effective for improving the horny surface of the skin due to the texture of the powder, and the surface of the soap is not roughened.

The natural oils in the present invention are natural oils including olive fruit oil, Jojoba seed oil, Camellia seed oil, Brown rice oil and Avocado oil .

Specifically, olive oil contains vitamin E and linoleic acid. It is a natural oil that prevents skin aging by antioxidative action, and contains polyphenol and phytosterol ingredients to prevent skin damage.

Jojoba oil is the oil most similar to human sebum structure, so it has excellent skin affinity. It is a natural oil that is gentle to the skin and moisturizes, has a soothing effect on the skin and has antimicrobial effect and is effective in improving atopic skin.

Camellia oil has a high oleic acid content, which is a natural moisturizing ingredient. It has excellent antioxidative effects because it contains vitamin E and phenolic compounds such as tyrosol and hydroxytyrosol. It also inhibits the growth of Staphylococcus aureus, which causes itching of the skin, and it is a natural oil that can alleviate itching of the skin because it contains gamma linolenic acid.

Brown rice oil contains gamma orizanol, vitamin E, omega-6 and omega-9 unsaturated fatty acids and is effective in preventing skin aging. It is rich in tocopherols and minerals and is a natural oil for skin nutrition and blood circulation.

Avocado oil contains sterol linin that suppresses skin spots and is rich in vitamins A, D and E, effective for preventing skin aging, and contains unsaturated fatty acids such as protein and omega 3, It is a natural oil effective to relieve the dryness and itching of the skin.

Specifically, the natural oil is preferably used in an amount of 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base, because it can effectively exhibit the components of the natural oil.

More specifically, 20 to 40% by weight of olive oil, 20 to 40% by weight of Jojoba oil, 20 to 30% by weight of camellia oil, 10 to 15% by weight of brown rice oil and 5 to 10% by weight of avocado oil Can be used. Within the above range, each component of the natural oil can be effectively retained, and it is possible to alleviate the dryness and itching of the skin caused by moisturizing and atopic dermatitis of the skin, and is also effective in preventing skin aging.

In the present invention, Aloe Barbadensis Leaf Extract contains polysaccharides such as pectic acid and glucomannans and various other organic and inorganic compounds. In addition, it has wound healing effect, it is excellent in cell regeneration and nutrient supply, has excellent moisture retention, and has an effect of inhibiting melanin pigment formation. It also contains a component called alfrogen, which is effective in suppressing allergies. The solids content of the extract may be from 0.1 to 5% by weight, but is not limited thereto.

Specifically, the aloe vera leaf extract is preferably used in an amount of 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base. Within the above range, it is preferable that the allergen is suppressed and the moisturizing power of the skin is increased and the viscosity is not affected during the production of the soap.

In the present invention, propolis is a variety of compounds and contains many minerals. In particular, it contains an abundance of Flavonoid, and it is an excellent material for alleviating the inflammation of atopic dermatitis and soothing the skin, because it can increase the immunity of the skin due to antibacterial action against fungi, bacteria and viruses.

Specifically, the propolis may be used in an amount of 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base. The antibacterial property of the soap composition can be increased within the above range.

In the present invention, honey is rich in nutrients such as various vitamins, minerals, amino acids and organic acids. Potassium in honey helps to drain the waste of blood into the body. Since honey and potted plants and enzymes are intact, they excel in the treatment of inflammation of the skin and are also good for removing dirt. In addition, the mineral component of honey can make rough skin smooth.

Specifically, honey can be used including one or two or more selected from acacia honey, oil honey, honey and sundried honey, but it is preferable to use acacia honey.

More specifically, honey is preferably used in an amount of 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base. The anti-inflammatory action and the moisturizing power of the soap composition can be enhanced within the above range.

In the present invention, shea butter is a natural moisturizing substance having abundant natural fatty acid and having the best moisture resistance among plant materials. It contains vitamins A, E, F and essential oxidized fatty acids, so it can supply nutrition and elasticity to the skin and effectively work on dry skin. In addition, it has excellent antioxidant function and can suppress skin aging.

Specifically, the shea butter preferably contains 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base. The moisture resistance of the soap composition and the smoothness of the skin can be increased within the above range.

In the present invention, coconut milk has little side effects on the skin and is effective for moisturizing the skin. Therefore, it is widely used as a skin care product. In addition, it is effective for treating various skin diseases such as psoriasis, eczema and dermatitis.

Specifically, the coconut milk is preferably used in an amount of 1 to 5 parts by weight, more preferably 2 to 4 parts by weight, based on 100 parts by weight of the soap base. The moisture resistance of the soap composition can be increased within the above range.

In the present invention, the fragrance may be any one selected from fruit incense such as red ginseng, aloe incense, bergamot incense, coconut incense, avocado incense, orange incense, lemon incense, vanilla incense, chocolate incense, It is preferable to use the red ginseng flavor because the red ginseng fermentation powder can be emphasized.

Specifically, the perfume may be used in an amount of 0.5 to 3 parts by weight, preferably 1 to 2 parts by weight, based on 100 parts by weight of the soap base. It is preferable to increase the fragrance of the soap within the above-mentioned range, thereby increasing the satisfaction of using the soap.

In the present invention, a red ginseng soap composition for improving atopic dermatitis can be prepared by further containing vegetable cellulose. Concretely, one or more selected from hydroxyethyl cellulose, hydroxypropyl methyl cellulose (HPMC), carboxymethylcellulose, methyl cellulose (MC) and the like, Can be used. The above-mentioned substances are hydrophilic and can be preferable because they are less irritating because they are materials that do not cause allergies.

More specifically, 0.1 to 5 parts by weight, preferably 1 to 3 parts by weight, of vegetable cellulose is preferably used for 100 parts by weight of the soap base. The detergency is improved within the above range, so that it is possible to produce a soap which is effective for removing keratin and improved smooth and smooth touch.

In the present invention, a red ginseng soap composition for improving atopic dermatitis can be prepared by further containing a natural preservative. Specifically, it may be selected from natural vitamin E, grapefruit seed extract, tocopherols and rosemary oil extract (ROE), and may be used alone or in combination of two or more. The grapefruit seed extract may have a solid content of 0.1 to 5% by weight and a grapefruit seed extract: vegetable glycerin in a 7: 3 ratio.

More specifically, it is preferable to use 0.1 to 5 parts by weight, preferably 0.1 to 2 parts by weight, of a natural preservative relative to 100 parts by weight of the soap base. Within the above range, it may be effective to extend the shelf life of the soap by the antioxidant properties of the soap and antiseptic and antimicrobial properties.

In the present invention, if necessary, the red ginseng soap composition for improving atopic dermatitis may further contain a natural pigment. Specifically, the natural coloring matter may be selected from beryllium coloring matter, paprika extracting color, grape skin extracting color, red cabbage coloring, gardenia yellowing color, squid ink coloring, cochineal coloring, purple sweet potato coloring and the like, . More specifically, 1 to 5 parts by weight of a natural pigment may be further added to 100 parts by weight of the soap base.

(A) adding water and a coenzyme to a red ginseng by-product, reacting the mixture at 40 to 60 ° C for 30 minutes to 30 hours, concentrating the supernatant under reduced pressure and drying to obtain a red ginseng fermented powder (B) adding a strain to the screen wind extract, reacting the mixture at 20 to 40 ° C for 5 to 50 hours, and concentrating under reduced pressure and fermenting to prepare a screened fermented extract; (c) Aging the mixture of the fermentation powder and the mixture of the fermented extract of the screen-wind produced in step (b) and the bokbunja extract at 30 to 50 ° C for 5 to 20 hours, (d) adding a soap base, a humectant and water to the stirrer, Adding the aged mixture of step (c), stirring the mixture, and injecting and coagulating the mixture into a soap frame.

The screening extract and the bokbule extract may be prepared by a conventional extraction method. Specifically, water extraction, alcohol extraction, organic solvent extraction, supercritical extraction and the like may be used. Preferably, water, alcohol, desirable. The water is preferably water or hot water at room temperature. As the extraction method, any one or more selected from shaking extraction, Soxhlet extraction, and reflux extraction may be used, but the present invention is not limited thereto.

In the step (a), the step of producing the coenzyme obtained from the malt may be performed first. Specifically, the malt is immersed in water for 1 to 3 hours. At this time, it is preferable to use distilled water of primary to tertiary water. To increase the leaching efficiency, distilled water can be repeated several times over two or more times using 2 to 5 times the weight of malt. The leaching temperature is preferably 15 to 35 占 폚, more preferably 20 to 30 占 폚. The filtrate is then pooled and precipitated in starch for 20 to 30 hours. The efficiency of obtaining enzymes such as α-amylase can be increased through the precipitation of starch. Thereafter, the precipitated solution is filtered under reduced pressure, and the obtained substance is lyophilized to prepare a coenzyme. The prepared coenzyme may be diluted with distilled water to a concentration of 0.01 to 3% by weight and used for preparing fermented red ginseng powder. It is preferable to increase the polysaccharide yield of the red ginseng by-product within the above range.

The produced coenzyme and red ginseng by-products are put into distilled water and reacted in a shaking incubator at 40 to 60 ° C. At this time, the number of revolutions is preferably 100 to 150 rpm. The reaction time can be from 30 minutes to 30 hours. As the reaction time increases within the above range, the polysaccharide yield can be increased. The by-products of red ginseng used herein include any one selected from red ginseng roots and red ginseng star or a mixture thereof.

The distilled water is preferably used in an amount of 5 to 15 parts by weight per 1 part by weight of the red ginseng by-product. The crude enzyme may be diluted with distilled water to a concentration of 0.01 to 3% by weight, and then added at a weight ratio of 2: 8 to 8: 2 of red ginseng byproduct to coenzyme to react to prepare red ginseng fermented powder.

After the reaction of the red ginseng by-product and the coenzyme, the autoclave is treated at 90 to 100 ° C for 5 to 20 minutes to deactivate the coenzyme. The treated solution is then centrifuged. The centrifugation is carried out using a centrifuge at a rotation number of 2500 to 3000 rpm for 10 to 30 minutes. The resulting supernatant can be concentrated using a vacuum concentrator at a temperature range of 30 to 40 ° C to increase the active ingredient content of red ginseng to a weight.

At this time, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration, but it is not limited thereto. And then dried to prepare red ginseng fermented powder. In this case, the red ginseng fermentation powder can be prepared by freeze drying, spraying or hot air drying, but not limited thereto.

In the step (b), the strain is added to the screen wind extract, reacted at 20 to 40 ° C for 5 to 50 hours, concentrated under reduced pressure, and fermented to prepare a screen-fed fermented extract.

The strain is Lactobacillus plantarum plantarum , Lactobacillus casei , and Streptococcus lactis may be used as the strain. The screening fermentation extract can be produced by mixing the screening extract: strain at a weight ratio of 1: 9 to 9: 1.

The prepared screen-fermented extract can be used after being treated in an autoclave at 90 to 100 ° C for 5 to 20 minutes to inactivate the strain. In this range, the active ingredient of the screen-fermented extract may not be destroyed. Thereafter, it is possible to concentrate under reduced pressure. It can be concentrated using a vacuum concentrator at a temperature range of 30 to 40 ° C.

Thereafter, filtration may be further performed by a conventional method. Filtration can be performed one or more times using a 0.1 to 10 mu m filter.

In the step (c), the red ginseng fermentation powder prepared in step (a) and the mixture of the fermentation extract and the bokbule extract prepared in step (b) are aged at a temperature of 30 to 50 ° C for 5 to 20 hours . The mixing is preferably carried out using a stirrer for 30 minutes to 2 hours, and the mixed mixture is aged at a temperature of 30 to 50 DEG C for 5 to 20 hours.

The mixture may be used in an amount of 2 to 30 parts by weight based on 100 parts by weight of the following soap base.

In the step (d), a soap base, a moisturizing agent, and water are added to the stirrer, followed by heating and emulsification. The aged mixture of step (c) is added and stirred.

Specifically, a soap base, a humectant and water are heated at 65 to 80 DEG C and mixed for 2 to 3 hours to emulsify the mixture in an agitator for soap production. At this time, it is preferable to use purified water as the water. The soap base is preferably used including sodium laurate, sodium stearate, sodium myristate, sodium laureth sulfate and a basic solution.

Thereafter, the temperature of the reactor is lowered to 40 to 50 DEG C, and then the aged mixture is added to the emulsified soap base and stirred in step (c). At this time, red ginseng powder, aloe vera leaf extract, propolis, honey, natural oil, shea butter, coconut milk and fragrance can be further added and stirred. The stirring time is preferably 1 to 3 hours. In the above stirring temperature range, the ingredients of each additive material are not destroyed, and the saponification reaction can occur well.

The stirred soap composition can then be injected into a soap mold and solidified. At this time, the secondary agitated composition may be filtered by a wire mesh of 110 to 130 mesh before being injected into the soap frame to remove foreign matter. The filtered soap composition is injected into a soap frame and then dried at 15 to 25 DEG C for 3 to 7 days to check for the air bubbles and moisture evaporation of the soap.

The coagulated soap may be discharged from the soap mold, cut using a soap cutting machine, and molded into a soap mold. At this time, the soap is prepared by cutting to 70 to 120 weight (g). The cut soap is put into a soap mold and compressed at a pressure of 40 to 150 kgf / cm 2 using a soap molding machine to form the shape of the soap. Thereafter, the molded soap may further include a step of selecting and wrapping the defective product using an automatic packing machine, a step of sticking the packaged soap, and packaging the packaged product in an out-box.

Hereinafter, the red ginseng soap composition for improving atopic dermatitis according to the present invention and the method for producing soap will be described in more detail. It should be understood, however, that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention.

Unless otherwise defined, all technical and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

In addition, the following drawings are provided by way of example so that those skilled in the art can fully understand the spirit of the present invention. Therefore, the present invention is not limited to the following drawings, but may be embodied in other forms, and the drawings presented below may be exaggerated in order to clarify the spirit of the present invention.

Also, the singular forms as used in the specification and the appended claims are intended to include the plural forms as well, unless the context clearly indicates otherwise.

[Preparation Example 1] Production of coenzyme obtained in malt

200 g of malt was leached in 500 ml of distilled water for 2 hours. At this time, the leaching is repeated three times. The obtained filtrate is precipitated in starch for 24 hours. Thereafter, the precipitate was filtrated under reduced pressure, and then lyophilized for 5 hours to prepare crude enzyme obtained from malt.

[Preparation Example 2] Preparation of fermented red ginseng powder by enzyme treatment

100 g of red ginseng roots dried as a by-product of red ginseng and 100 g of coenzyme are added to 900 ml of distilled water. The crude enzyme was diluted to a concentration of 0.01% by weight (w / w) using distilled water and added to the crude enzyme prepared in Preparation Example 1, followed by addition. This solution is placed in a shaking incubator at 50 ° C and rotated at 150 rpm. At this time, the reaction time is 1 hour, 3 hours, 5 hours, 7 hours, 12 hours, 24 hours. For inactivation of the coenzyme, the reacted solution is placed in an autoclave and treated at 95 ° C for 10 minutes. The fermented red ginseng powder was centrifuged at 3000 rpm and 10 ml of the supernatant was lyophilized at -20 ° C.

[Production Example 3]

The crude enzyme was diluted with distilled water to a concentration of 0.1 wt% (w / w), and all the steps were carried out in the same manner as in Preparation Example 2.

[Production Example 4]

The crude enzyme was diluted to 1 wt% (w / w) using distilled water, and all the steps were carried out in the same manner as in Preparation Example 2. Based on the sample of Preparation Example 4, the immunological activity of Experimental Example 2 was evaluated.

[Production Example 5]

The crude enzyme was diluted with distilled water to 3 wt% (w / w), and all the steps were carried out in the same manner as in Preparation Example 2.

[Production Example 6]

The coenzyme was diluted with 5 wt% (w / w) using distilled water, and all the steps were carried out in the same manner as in Preparation Example 2.

[Production Example 7]

10 g of red ginseng starch was used as a by-product of red ginseng, and the coenzyme was diluted to 1 part by weight (w / w) using distilled water and all the steps were carried out in the same manner as in Preparation Example 2.

[Preparation Example 8] Preparation of red ginseng powder

Red ginseng powder was prepared by pulverizing 80 g of red ginseng roots and 20 g of red ginseng starch in an oven at 40 ° C for 48 hours to 80 mesh.

[Preparation Example 9] Preparation of bokbunja extract

1000 ml of a mixture of ethanol and water in a volume ratio of 5: 5 was added to 500 g of bokbunja, and the mixture was extracted three times at 60 캜 with a reflux condenser at room temperature for 2 hours. After filtration using a filter paper (Whatman No. 6), the filtrate was concentrated under reduced pressure to prepare a brambly extract having a solid content of 2% by weight.

[Production Example 10] Production of screen-fermented fermented extract

A folding screen ( Centella asiatica ) was added to 1000 ml of a mixed solution of ethanol and water in a volume ratio of 5: 5, and the mixture was extracted three times at 60 ° C using a reflux condenser at room temperature for 2 hours. After filtration using a filter paper (Whatman No. 6), the filtrate was concentrated under reduced pressure to prepare a screen wind extract having a solid content of 1 wt%.

90% by weight of the prepared screen wind extract and 10% by weight of Lactobacillus plantarum strain were added and reacted at 30 DEG C for 10 hours. Then, in order to inactivate the strain, the mixture was treated in an autoclave at 90 DEG C for 15 minutes Then, the mixture was concentrated using a vacuum concentrator and filtered using a 5 탆 filter to prepare a screen-dried fermented extract.

[Comparative Production Example 1] Fermented red ginseng powder by enzyme treatment

All the steps were carried out in the same manner as in Production Example 2, except that coenzyme was not used.

[Experimental Example 1] Measurement of yield of fermented red ginseng powder

(1) Determination of yield by enzymatic treatment of by-products of red ginseng

1 ml of the supernatant prepared in Preparative Examples 2 to 7 and Comparative Preparative Example 1 was lyophilized at -20 ° C, and the yield was calculated by measuring the weight. The yield was calculated by the following equation.

Yield (%) = [(weight after freeze drying g) / (weight before freeze drying g)] × 100

(2) Measurement of polysaccharide content

8 ml of 99% ethanol was added to 2 ml of the supernatant prepared in Preparative Examples 2 to 7 and Comparative Preparative Example 1, and the polysaccharide was precipitated at room temperature for 24 hours. Then, the supernatant was removed by centrifugation at 3,000 rpm for 20 minutes, The resulting polysaccharide was lyophilized at -20 ° C and then the yield was calculated. The yield was calculated by the following equation.

Yield (%) = [(Weight after freeze drying g) / (Weight of supernatant prepared g)] × 100

(3) Experimental results

Coenzyme yield(%) density(%) Reaction time (h) One 3 5 7 12 24 Production Example 2 0.01 33 34 34 35 35 36 Production Example 3 0.1 36 35 36 37 37 43 Production Example 4 One 35 39 42 43 46 55 Production Example 5 3 37 46 50 50 52 61 Production Example 6 5 42 49 55 54 52 65 Production Example 7 One 34 45 50 52 53 55 Comparative Preparation Example 1 0 32 34 33 31 31 36

Table 1 is a table showing the yields of fermented red ginseng powder prepared in Production Examples 2 to 7 and Comparative Production Example 1. As shown in Table 1, it can be seen that as the concentration of coenzyme increases in Production Examples 2 to 6 and the reaction time increases, the yield of red ginseng roots increases. Production Example 7 showed the yield of red ginseng starch from the red ginseng fermented powder. Comparative Production Example 1 shows that the yield is not high because no coenzyme is used.

Coenzyme Polysaccharide yield (%) density(%) Reaction time (h) One 3 5 7 12 24 Production Example 2 0.01 22 24 21 22 23 25 Production Example 3 0.1 22 24 25 26 27 30 Production Example 4 One 23 25 26 27 28 35 Production Example 5 3 22 24 26 25 26 30 Production Example 6 5 23 25 24 24 26 27 Production Example 7 One 24 28 32 34 35 36 Comparative Preparation Example 1 0 21 24 22 20 21 25

Table 2 shows the polysaccharide yields of red germ fermented powders prepared in Production Examples 2 to 7 and Comparative Production Example 1. As shown in Table 2, in Production Examples 2 to 6, the yield of the red ginseng polysaccharide was increased as the concentration of the coenzyme increased and the reaction time increased. In the case of Production Example 6, 5% by weight of coenzyme was added, but the yield of red ginseng polysaccharide was not higher than that of Production Example 3. [ Production Example 7 shows the yield of the red ginseng starch polysaccharide in the fermented red ginseng powder and that the yield of the polysaccharide of the red ginseng starch was higher than that of the red ginseng extract. Comparative Production Example 1 does not use a coenzyme and thus it can be confirmed that the polysaccharide yield is not high.

[Experimental Example 2] Evaluation of Immune Activity of Fermented Red Ginseng Powder

The immunological activity of Experimental Example 2 was evaluated on the basis of the sample of Preparation Example 4 which was reacted for 24 hours.

(1) Cell culture

Raw 264.7 cells, a mouse macrophage cell line, were purchased from Korean Cell Line Bank (KCLB) and used for DMEM (Dulbeco's Modified Eagle's Media) medium containing 10% FBS (Fetal Bovine Serum) and 1% penicillin-streptomycin Respectively. Cells were cultured at 37 ° C and 5% CO ₂ .

(2) Measurement of cell viability

Toxicity to cells was measured by MTT (3- (4,5-dimethylthiazol-2-yl) -

2,5-diphenyltetrazolium bromide) method. Raw 264.7 cells were plated at 2 × 10 ⁴ cells / ml in 96-well plates and the samples were treated with 25, 50, 100, and 200 μg / ml for 24 hours. 10 μl of the solution was added to each well using EZ-Cytox (Daeil, Korea) and reacted at 37 ° C and 5% CO ₂ for 30 minutes. Then, microplate reader (Molecular devices, USA) The change in absorbance at 450 nm was measured and the cell viability for the control group was expressed as a percentage.

(3) Measurement of nitric oxide production

NO concentration was determined by using the NO assay kit (Intron, Korea). Raw 264.7 cells were adjusted to 2 × 10 ⁴ cells / mL using DMEM (Dulbecco's Modified Eagle's Media), and then seeded in 96-well plates and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. After incubation, the cells were washed with PBS (Phosphate-Buffered Saline). LPS (Lipopolysaccharides) at 1 μg / mL and 25, 50, 100 and 200 ㎍ / ㎖ were added as a positive control and cultured for 24 hours. 50 μl of sulfanilamide in the reaction buffer was added using a nitrogen oxide detection kit (Intron, Korea) and reacted for 10 minutes. After incubation at room temperature for 10 minutes with 50 μl of Naphthylethylenediamine in the stabilizer buffer, the absorbance was measured at 540 nm in an enzyme-linked immunosorbent assay reader (Molecular devices, USA). The absorbance was measured from the calibration curve of sodium nitrite The production of nitrite, a metabolite of NO, was calculated.

(4) Measurement of TNF-α production

The amount of TNF-α produced in the cell culture was measured by ELISA (enzyme-linked immunosorbent assay) kit. Raw 264.7 cells were seeded in a 6-well plate at 2 × 10 ⁴ cells / ml using DMEM (Dulbecco's Modified Eagle's Media) medium and cultured at 37 ° C and 5% CO ₂ for 24 hours. Cells were treated with 1 μg / mL of LPS (Lipopolysaccharides) and 0, 25, 50, 100, 200 μg / mL samples as a positive control and cultured for 24 hours. TNF (Tumor necrosis factors) -α (BD, USA) was measured at 450 nm using an ELISA reader (Molecular devices, USA).

(5) Measurement of inflammation-related protein expression patterns in cells

To determine the inflammatory effect, the amount of iNOS and COX-2 protein associated with inflammation was measured. The cells were cultured on a 6-well plate at 2 × 10 ⁴ cells / ml using a macrophage cell line (Raw 264.7), treated with PBS, washed with PBS (Phosphate-Buffered Saline) 24 hours later, And cell lysate was prepared using RIPA buffer. The amount of protein contained in the cell lysate was measured by the Bradford method. 20 μg of the protein was separated by SDS-PAGE containing 10% polyacrylamide and electroblotted with PVDF (polyvinylidene fluoride) membrane at 120 V for 1 hour. The PVDF membrane was then left in 5% skim milk for 1 hour and then reacted overnight at 4 ° C in the primary antibody. After washing three times with tris-buffered saline and tween 20, secondary antibody was reacted at room temperature for 1 hour, washed three times with TBST, and then treated with ECL (Amersham-Pharmacia) , USA), and band phenomena were measured using Chemidoc (Bio-Rad, USA).

(6) Measurement of splenocyte proliferation

Spleen cells of mice were used to measure the effect on the proliferation of immune cells. First, the spleen of the mouse was separated, and then pulverized through a mesh and centrifuged to remove the supernatant, and the spleen cells were recovered. The remaining red blood cells were removed with RBC (Red Blood Cell) lysis buffer and then washed twice with RPMI (Rosewell Park Memorial Institute) 1640 medium. The isolated splenocytes were diluted to 5 × 10 ⁶ cells / ml. After placed in a 96 well plate, and put to one, insert the concanavalin A (Con A) is a lipopolysaccharide (LPS) and B cell activation inducer activated inducer T cell dilute the sample at different concentrations for 48 hours 5%, CO ₂ incubator and cultured in an incubator. Then, 10 μl of CCK-8 kit (Dojindo Laboratories, Japan) solution was added to each well, incubated for 4 hours at 37 ° C in a 5% CO ₂ incubator, and the absorbance was measured at 450 ㎚.

(7) Experimental results

As shown in FIG. 1, the fermented red ginseng powder of Preparation Example 4 according to the present invention showed cell viability of 98.0% at a high concentration of 200 μg / ml in RAW 264.7 cells, showing no toxicity to immune cells Respectively. The control in Fig. 1 is a control group in which the sample is not treated.

As shown in Fig. 2, the amount of NO produced was 8.9 uM in the control group without treatment, and the amount of NO production was increased to 32.8 uM when induced by LPS. In the group treated with 25 ㎍ / ㎖, 50 ㎍ / ㎖ and 100 ㎍ / ㎖ of red ginseng powder of Preparation Example 4, the production of NO was significantly suppressed to 16.1, 17.2 and 18.0 uM in p <0.05 compared with the control group There was an anti-inflammatory effect.

As shown in FIG. 3, the amount of pro-inflammatory cytokine TNF-α produced in RAW 264.7 cells was found to be 64.1 ng / ml in the control group without treatment, ng / ml. TNF-α production was inhibited at 88.2, 140.7, and 154.3 in the groups treated with 25 μg / ml, 50 μg / ml and 100 μg / ml of the red ginseng powder of Preparation Example 4, respectively, .

[Example 1] Red ginseng soap for improving atopic dermatitis

20% by weight of sodium laureate, 20% by weight of sodium stearate, 20% by weight of sodium premisteed, 10% by weight of sodium laureth sulfate and 30% by weight of triethanolamine were used as soap bases.

5 parts by weight of the red ginseng fermentation powder prepared by reacting in the production example 4 for 24 hours with respect to 100 parts by weight of the soap base, 0.5 part by weight of the bramble extract prepared in Preparation Example 9, and 1 part by weight of the fermentation extract prepared in Preparation Example 10 After mixing for 3 minutes on a stirrer, aging was performed for 10 hours to prepare aged material.

The soap base was charged into a stirrer. To 100 parts by weight of the soap base, 10 parts by weight of glycerin and 6 parts by weight of sorbitol were added as a humectant, and 10.5 parts by weight of purified water was added. After heating at 70 DEG C to emulsify the soap base for 1 hour, 1 part by weight of the aged material and the perfume was added at 45 DEG C and the mixture was stirred for 2 hours.

The agitated soap composition was removed by using a 120-mesh wire net, and the soap frame was then dried for 4 days to solidify. The clotted soap was taken out from the soap mold and cut into 80 g using a soap cutter. The cut soap was placed in a soap mold and compressed at a pressure of 100 kgf / cm 2 using a soap molding machine to form the shape of the soap. The thus-produced soap was subjected to sensory evaluation according to Experimental Example 3, and the results are shown in Table 4. [ The content of the manufactured soap is shown in Table 3.

[Example 2]

The red ginseng fermented powder produced by the reaction in Production Example 4 for 24 hours, the bergamot extract prepared in Preparation Example 9 and the fermented fermentation extract prepared in Preparation Example 10 were mixed, Was carried out in the same manner as in Example 1. At this time, 10.5 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Example 3]

All the steps were carried out in the same manner as in Example 1 except that 10 parts by weight of fermented red ginseng produced by reacting in Production Example 4 for 24 hours with respect to 100 parts by weight of the soap base and 5.5 parts by weight of purified water were used. The content of the manufactured soap is shown in Table 3.

[Example 4]

All the steps were carried out in the same manner as in Example 1, except that 1 part by weight of fermented red ginseng produced by reacting in Production Example 4 for 24 hours and 14.5 parts by weight of purified water were used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Example 5]

Except that 0.8 parts by weight of red ginseng powder was further added to 100 parts by weight of the soap base and 9.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 6]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of aloe vera leaf extract having a solid content of 0.1% was further added to 100 parts by weight of the soap base and 8.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 7]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of propolis was further added to 100 parts by weight of the soap base and 7.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 8]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of acacia honey was further added to 100 parts by weight of the soap base and 6.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 9]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of shea butter was further added to 100 parts by weight of the soap base and 5.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 10]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of coconut milk was further added to 100 parts by weight of the soap base and 4.7 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 11]

All the steps were carried out in the same manner as in Example 1 except that 1 part by weight of natural oil was further added to 100 parts by weight of the soap base and 3.1 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 12]

Except that 5 parts by weight of vegetable cellulose was further added to 100 parts by weight of the soap base and 1.1 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 13]

5 parts by weight of red ginseng fermentation powder prepared by the reaction of red ginseng in Production Example 4 for 24 hours and 100 parts by weight of the red ginseng fermentation powder 5 Except that 10 parts by weight of fermented red ginseng powder was used and 3.1 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Example 14]

All the steps were carried out in the same manner as in Example 1 except that 0.1 part by weight of the bokbunja extract prepared in Preparation Example 9 and 11.9 parts by weight of purified water were used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Example 15]

All the steps were carried out in the same manner as in Example 1 except that 0.1 part by weight of the screen-fermented extract prepared in Preparation Example 10 and 12.4 parts by weight of purified water were used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Comparative Example 1] Red ginseng soap for improving atopic dermatitis

All the steps were carried out in the same manner as in Example 1, except that the brambone extract prepared in Preparation Example 9 was not used. At this time, 3.6 parts by weight of purified water was used for 100 parts by weight of the soap base.

[Comparative Example 2]

All the steps were carried out in the same manner as in Example 1, except that the red ginseng fermented powder prepared in Preparation Example 4 was not used. At this time, 8.1 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Comparative Example 3]

All the steps were carried out in the same manner as in Example 1, except that the screen-fermented extract prepared in Preparation Example 10 was not used. At this time, 4.1 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Comparative Example 4]

All the processes were carried out in the same manner as in Example 1, except that the bokbunja extract prepared in Preparation Example 9 and the screen-fermented extract prepared in Preparation Example 10 were not used. At this time, 4.6 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Comparative Example 5]

All processes were carried out in the same manner as in Example 1, except that the red ginseng fermentation powder prepared in Preparation Example 4 and the fermentation extract prepared in Preparation Example 10 were not used. At this time, 9.1 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Comparative Example 6]

All the processes were carried out in the same manner as in Example 1, except that the red ginseng fermented powder prepared in Preparation Example 4 and the bokbunja extract prepared in Preparation Example 9 were not used. At this time, to 100 parts by weight of the soap base, 8.6 parts by weight of purified water was used. The content of the manufactured soap is shown in Table 3.

[Comparative Example 7]

All the processes were carried out in the same manner as in Example 1 except for the red ginseng fermentation powder prepared in Preparation Example 4, the bokbunja extract prepared in Preparation Example 9, and the fermentation extract prepared in Preparation Example 10. At this time, 9.6 parts by weight of purified water was used for 100 parts by weight of the soap base. The content of the manufactured soap is shown in Table 3.

[Experimental Example 3] Sensory evaluation for patients with atopic dermatitis

(1) Evaluation method

The soaps prepared in Examples 1 to 15 and Comparative Examples 1 to 7 were tested. A total of 220 male patients and 110 female patients with atopic dermatitis randomly selected from all over the country were divided into 22 groups of 5 male and 5 female patients, The effect was evaluated using the soap prepared in 7. The use of soap was applied to the skin showing symptoms of atopic dermatitis, twice daily in the morning and evening, and for 1 to 3 minutes in 20 ° C tap water. One month after the start of the test, the skin condition was observed and the effect was evaluated. The evaluation items were evaluated for cleansing power, moisturizing power, skin smoothness, skin irritation, wound healing power, itching relief and dermatitis relief. The detergency was evaluated by the cleaning effect of foreign substances on skin surface, keratin and cosmetics. The moisturizing power was evaluated by using a skin moisture meter (Skin Analyzer-Moritex Corp.Japan) on the skin after use of the soap. The smoothness of the skin was evaluated by the user's subjective degree of skin roughness and smoothness after use of the soap. Skin irritation was evaluated after observing skin redness and skin pulling after using soap. The wound healing power was evaluated by observing the healing effect of cracked skin dried by atopic dermatitis. Itching evaluated the degree to which skin itching caused by atopic dermatitis was alleviated after using soap. Dermatitis relief was assessed after observing the extent to which dermatitis caused by atopic dermatitis was alleviated after use of the soap. The evaluation method was divided into 10 points and evaluated as 2 points very poor, 4 points worse, 6 points average, 8 points good, 10 points very good, and then expressed as a whole average value. The results are shown in Table 4.

(2) Experimental results

Weight portion Remarks Red ginseng
Fermented powder Bokbunja
extract Fold-wind fermented extract Red ginseng powder Aloe vera leaf extract Propolis honey Shea Butter Coconut milk Natural oil Vegetable cellulose water Example 1 5 0.5 One 0 0 0 0 0 0 0 0 10.5 Example 2 5 0.5 One 0 0 0 0 0 0 0 0 10.5 Aging X Example 3 10 0.5 One 0 0 0 0 0 0 0 0 5.5 Example 4 One 0.5 One 0 0 0 0 0 0 0 0 14.5 Example 5 5 0.5 One 0.8 0 0 0 0 0 0 0 9.7 Example 6 5 0.5 One 0.8 One 0 0 0 0 0 0 8.7 Example 7 5 0.5 One 0.8 One One 0 0 0 0 0 7.7 Example 8 5 0.5 One 0.8 One One One 0 0 0 0 6.7 Example 9 5 0.5 One 0.8 One One One One 0 0 0 5.7 Example 10 5 0.5 One 0.8 One One One One One 0 0 4.7 Example 11 5 0.5 One 0.8 One One One One One 1.6 0 3.1 Example 12 5 0.5 One 0.8 One One One One One 1.6 2 1.1 Example 13 5 0.5 One 0.8 One One One One One 1.6 0 3.1 Red ginseng fermented powder ingredient Example 14 5 0.1 One 0 0 0 0 0 0 0 0 11.9 Example 15 5 0.5 0.1 0 0 0 0 0 0 0 0 12.4 Comparative Example 1 5 0 One 0.8 One One One One One 1.6 0 3.6 Comparative Example 2 0 0.5 One 0.8 One One One One One 1.6 0 8.1 Comparative Example 3 5 0.5 0 0.8 One One One One One 1.6 0 4.1 Comparative Example 4 5 0 0 0.8 One One One One One 1.6 0 4.6 Comparative Example 5 0 0.5 0 0.8 One One One One One 1.6 0 9.1 Comparative Example 6 0 0 One 0.8 One One One One One 1.6 0 8.6 Comparative Example 7 0 0 0 0.8 One One One One One 1.6 0 9.6

Table 3 is a table showing compositions of red ginseng soap for improving atopic dermatitis prepared in Examples 1 to 15 and Comparative Examples 1 to 7. As shown in Table 3, in Examples 1 to 15 and Comparative Examples 1 to 7, each component was expressed in terms of parts by weight with respect to 100 parts by weight of the soap base, and 16 parts by weight of the moisturizer and 1 part by weight of red ginseng It is a composition table doing. In Example 2, the fermented red ginseng powder, the bokbunja extract and the screen-fungus fermentation extract were mixed, and the fermentation process was not performed. Examples 1 to 12, 14 to 15 and Comparative Examples 1 to 7 used fermented red ginseng powder extracted from red ginseng roots. The fermented red ginseng powder of Example 13 contained red ginseng fermented red ginseng extract Powder were mixed and used.

Evaluation items Cleaning power Moisture power lubricity Skin irritation Wound healing Itching relief dermatitis
ease Example 1 7.60 6.30 6.50 7.30 7.50 7.30 7.70 Example 2 7.30 6.10 6.20 6.60 6.00 5.90 6.10 Example 3 7.80 6.40 7.60 7.30 7.40 7.40 7.90 Example 4 6.90 6.10 6.00 6.20 6.20 6.50 6.30 Example 5 8.20 6.50 6.90 7.20 7.60 7.50 8.00 Example 6 8.10 7.00 7.20 7.40 7.80 7.90 8.30 Example 7 8.10 7.30 7.50 7.70 8.10 8.10 8.50 Example 8 8.30 8.00 7.90 8.00 8.40 8.50 8.70 Example 9 8.60 8.40 8.40 8.50 8.70 8.90 9.00 Example 10 8.80 8.90 8.70 8.70 9.10 9.20 9.30 Example 11 9.10 9.10 9.00 9.10 9.30 9.60 9.50 Example 12 9.60 9.40 9.70 9.30 9.40 9.60 9.40 Example 13 9.20 9.30 9.40 9.50 9.50 9.70 9.70 Example 14 7.40 5.90 6.10 6.90 6.60 6.80 6.30 Example 15 7.30 6.20 6.00 6.50 6.00 6.20 6.00 Comparative Example 1 8.90 7.60 7.20 5.90 6.30 5.00 6.40 Comparative Example 2 7.50 8.60 4.90 5.10 5.40 3.70 3.30 Comparative Example 3 7.60 8.90 6.50 5.90 4.70 5.40 6.50 Comparative Example 4 7.70 7.40 5.20 5.30 4.30 4.30 6.20 Comparative Example 5 7.30 7.20 4.90 5.20 1.50 2.00 1.80 Comparative Example 6 7.40 6.40 4.30 4.90 3.20 2.70 2.00 Comparative Example 7 7.10 6.40 3.80 4.20 1.20 1.90 1.70

Table 4 shows the results of sensory evaluation according to Examples 1 to 15 and Comparative Examples 1 to 7. As shown in Table 4, since the fermented red ginseng powder, the bokbule extract and the screen fermentation extract do not have a ripening process, as shown in Table 4, lower skin irritation, wound healing, itching relief and dermatitis It can be seen that the numerical value of the relaxation is shown.

Examples 1, 3 and 4 show the evaluation according to the content of fermented red ginseng powder. It can be seen that Example 3 in which the content of red ginseng fermentation powder is the highest is high in the overall evaluation.

In Example 5, red ginseng powder was further contained to increase cleansing power and smoothness, and it was found that it was also effective in relieving itching and dermatitis. Example 6 further shows that aloe vera leaf extract is further contained, which increases moisturizing power and smoothness, which is helpful for skin irritation itching and dermatitis relief. Example 7 further contains propolis which is excellent in antimicrobial activity, and it is found that it is effective for wound healing, itching and dermatitis relief. Example 8 further contains honey with a rich nutritional component, which shows improvement in moisturizing power and effect on wound healing, itching and dermatitis relief. Example 9 further contains shea butter, which is good for moisturizing, to improve the moisturizing power and smoothness of the skin, and is also effective for wound healing, itching and dermatitis relief. It can be seen that Example 10 contains more coconut milk which is good for moisturizing and treating skin diseases, and shows an improved value as a whole. Example 11 is characterized in that it is rich in unsaturated fatty acids and further contains natural oils such as Jojoba oil having antimicrobial function and camellia oil which is effective for alleviating itching, showing high moisture retention and smoothness, and being effective against wound healing, itching and dermatitis Able to know. It can be seen that Example 12 further contains vegetable cellulose and exhibits excellent washing power and skin smoothness. Example 13 was prepared by mixing red ginseng fermented powders prepared using red ginseng starch having a high polysaccharide content without using only red ginseng fermented powder prepared using red ginseng roots. Therefore, it is possible to obtain better wound healing power, The effect can be confirmed in mitigation.

In Example 14, the content of the bokbunja extract is low, which shows a relatively low value in moisturizing, smoothness, skin irritation, wound healing, itching and dermatitis relief.

In Example 15, the content of the fermented extract of the screen was low, which was relatively low in moisture, smoothness, and skin irritation, and was found to be particularly low in wound healing, itching and dermatitis relief.

Since Comparative Example 1 is not included, it can be seen that it is not effective for skin irritation, wound healing, itching relief and dermatitis relief, and it can be seen that the number of itching relief is greatly reduced.

In Comparative Example 2, the number of skin irritation, wound healing, itching, and dermatitis relief was greatly reduced because no red ginseng fermentation powder was included. In Comparative Example 3, the effect of wound healing was greatly reduced because no screen-fermented extract was contained. In Comparative Example 4, it was found that the bacterium extract and the screen-fermented extract were not included, and the skin smoothness was reduced and the effect of skin irritation, wound healing, itching and dermatitis relief was reduced. Comparative Example 5 did not include the red ginseng fermentation powder and the screening fermentation extract, and Comparative Example 6 contained no red ginseng fermentation powder and bokbunja extract, showing little effect of wound healing, itching and dermatitis relief. Comparative Example 7 shows that the components of the red ginseng fermentation powder, bokbunja extract, and screen fermentation extract are not all included and thus the overall evaluation shows a very low value.

Therefore, it can be confirmed that the soap containing the fermented red ginseng powder produced by the present invention is effective in improving atopic dermatitis. In addition, it can be seen that it is preferable that the red ginseng fermentation powder, the bokbunja extract, and the screening fermentation extract are all included and aged. Also, when the soap containing red ginseng powder, aloe vera leaf extract, propolis, honey, shea butter and coconut milk is manufactured and used, excellent detergency, moisturizing power, smoothness of skin, wound healing, relieving itching, It can be confirmed that the most effective when the vegetable cellulose is further contained. From this, it can be confirmed that the red ginseng soap produced by the present invention is excellent in the improvement of atopic dermatitis.

Claims (16)

A red ginseng soap composition for improving atopic dermatitis comprising red ginseng fermentation powder, bokbunja extract, and screened fermentation extract. The method according to claim 1,
2. A red ginseng soap composition for improving atopic dermatitis, comprising 100 parts by weight of a soap base, and 2 to 30 parts by weight of a mixture containing red ginseng fermentation powder, bokbunja extract, and screened fermentation extract. 3. The method of claim 2,
Wherein the mixture comprises 60 to 80 wt% of red ginseng fermentation powder, 1 to 10 wt% of bokbunja extract, and 10 to 30 wt% of fennel fermentation extract. 3. The method of claim 2,
10 to 40 parts by weight of a moisturizer, 0.1 to 15 parts by weight of red ginseng powder, 1 to 5 parts by weight of natural oil, 1 to 5 parts by weight of an aloe vera leaf extract, 1 to 5 parts by weight of propolis, 1 to 5 parts by weight of shea butter, 1 to 5 parts by weight of shea butter, 1 to 5 parts by weight of coconut milk, 1 to 20 parts by weight of water and 0.5 to 3 parts by weight of fragrance. The method according to claim 1,
Wherein the red ginseng fermentation powder is obtained by reacting a by-product of red ginseng with a coenzyme obtained from malt. 6. The method of claim 5,
Wherein the ginseng by-product comprises red ginseng root, red ginseng starch, or a mixture thereof. The method according to claim 1,
The above screen-dried fermented extract was prepared by extracting the leaves of the leaves and Lactobacillus plantarum plantarum , Lactobacillus casei and Streptococcus lactis . The present invention relates to a red ginseng soap composition for improving atopic dermatitis. 3. The method of claim 2,
A red ginseng soap composition for improving atopic dermatitis, which comprises 0.1 to 5 parts by weight of vegetable cellulose, a natural preservative or a mixture thereof, based on 100 parts by weight of a soap base. (a) adding water and a coenzyme to a red ginseng by-product, reacting the mixture at 40 to 60 ° C for 30 minutes to 30 hours, concentrating the supernatant under reduced pressure and drying to prepare a red ginseng fermentation powder,
(b) adding a strain to the screen wind extract and reacting at 20 to 40 ° C for 5 to 50 hours, concentrating under reduced pressure and fermenting to prepare a screen-fed fermented extract,
(c) aging the mixture of the red ginseng fermentation powder prepared in the step (a) and the fermented extract of the screenworm prepared in the step (b) and the brambone extract at 30 to 50 ° C for 5 to 20 hours,
(d) adding a soap base, a moisturizing agent and water to the stirrer, heating and emulsifying the mixture, adding the aged mixture of step (c) and stirring, then injecting and solidifying the stirred soap composition into a soap mold,
Wherein the method comprises the steps of: 10. The method of claim 9,
Wherein the mixture comprises 60 to 80% by weight of red ginseng fermentation powder, 1 to 10% by weight of bokbunja extract, and 10 to 30% by weight of fermented extract of folded persimmon in the step (c). 10. The method of claim 9,
In the step (a), the red ginseng by-product comprises red ginseng roots, red ginseng starch or a mixture thereof. 10. The method of claim 9,
In the step (a), the coenzyme is prepared by leaching malt into water, precipitating the filtrate for 20 to 30 hours in the starch, filtering under reduced pressure, and freeze-drying the red ginseng soap for improving atopic dermatitis Gt; 10. The method of claim 9,
In the step (a), the coenzyme is diluted with water to a concentration of 0.01 to 3% by weight and added. 10. The method of claim 9,
Wherein the red ginseng fermentation powder is used in a weight ratio of 2: 8 to 8: 2 by weight of the red ginseng by-product, and the coenzyme is used in the step (a). 10. The method of claim 9,
The method for producing at least one red ginseng soap for improving atopic dermatitis according to claim 1, wherein in step (b), the screenworm extract: strain is mixed at a weight ratio of 1: 9 to 9: 1. 10. The method of claim 9,
Wherein the red ginseng powder, the aloe vera leaf extract, the propolis, the honey, the natural oil, the shea butter, the coconut milk and the perfume are further added and stirred in the step (d).

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