KR20170089737A

KR20170089737A - Composition comprising nut fine extract for oral hygiene improvement and manufacturing method thereof

Info

Publication number: KR20170089737A
Application number: KR1020160035454A
Authority: KR
Inventors: 김선영; 한경찬; 유정식; 라문진; 정낙원
Original assignee: 재단법인 홍천메디칼허브연구소; 주식회사 하티
Priority date: 2016-01-27
Filing date: 2016-03-24
Publication date: 2017-08-04

Abstract

The present invention relates to a composition for promoting oral hygiene comprising a pine cones extract and a method for preparing the same, and more particularly, to a composition containing a pine cone extract, which exhibits excellent oral antibacterial activity and deodorizing ability, It is confirmed that it can be used variously.

Description

[0001] The present invention relates to a composition for enhancing oral hygiene in pine trees and a method for preparing the same,

The present invention relates to a composition for enhancing oral hygiene comprising a pine cones extract and a method for producing the same.

The teeth are minerals made of calcium phosphate. The disease is mainly tooth decay and periodontal disease. According to the Korean Dental Association, more than 90% of children experience dental caries and more than 80% of adults have gum disease. The major cause of this disease is infection by microorganisms in the mouth, which is caused by the interaction of bacteria, food, and saliva. That is, the nutrients and moisture required for the development of oral bacteria are continuously supplied by food and saliva, and the environment in the oral cavity has a temperature (37 ° C) and pH (neutral vicinity) suitable for the development of microorganisms. Streptococcus mutans and Prevotella intermedia are typical microorganisms causing periodontal disease. These microorganisms are formed on the tooth surface of insoluble glucan (Glucan), a polymer of glucose. In this process, glucan adheres to the tooth surface with other microorganisms in the oral cavity to form a dental plaque, ie, a dental plaque. Lactic acid accumulated by lactic acid bacteria including Streptococcus mutans in the formed plaque dissolves the enamel on the surface of the teeth, and tooth decay is generated. In addition, periodontal disease is caused by the products resulting from the propagation of such various microorganisms. To prevent tooth decay and periodontal disease, antimicrobial substances such as sodium bicarbonate (NaHCO ₃ ), triclosan (C ₁₂ H ₇ C ₁₃ O ₂ ), polyphosphate and sodium fluoride (NaF), vancomycin , Antibiotics such as chlorhexidine and spiramycin, or organic / inorganic fluorine have been used. However, the above methods are effective for preventing tooth decay, but their use is limited due to the disadvantage of vomiting, diarrhea, and antibiotic resistance. In particular, antibiotics used for bacterial infectious diseases such as tooth decay and periodontitis are not only harmful to the beneficial bacteria of the human body but also cause the occurrence of resistant bacteria, deterioration of immunity of the patient, chronicization of infectious diseases, It is known to cause the same side effects.

Therefore, it is necessary to develop a natural substance which has no side effects and has high antimicrobial activity selectively against harmful oral microorganisms.

On the other hand, halitosis is believed to cause more than 85% of oral malodor in oral cavity as a result of bacterial contamination of host components and food residues in oral cavity. There are several causes of oral cavity such as sickle, periodontal disease, impure mouth hygiene, food residue, improper prosthesis, unsanitary denture, oral cancer. Currently, the most important cause of bad breath is sore throat and periodontal disease. Volatile sulfur compounds (VSC) are the main causes of halitosis, including hydrogen sulfide (H2S), methyl mercaptan (CH3SH), and dimethyl sulfide (CH3SCH3) And methylmercaptine accounts for about 90%. These VSCs are mainly used for the corruption of anaerobic bacteria in the oral cavity using proteins (especially those containing cysteine and methionine), peptides, amino acids, etc. contained in the gingiva, saliva, saliva and food residues Lt; / RTI > Representative gram negative anaerobic bacteria include Porphyromonas gingivalis , Prevotella intermedia ( Prevotella intermedia , and Fusobacterium nucleatum . As a result, oral bacteria are believed to play an important role in halothane induction.

In order to prevent and control such bad breath, modern people use toothpaste, mouthwash and the like, but they only show a short-term effect and use fluorine compounds such as trichloroacid, chlorohexadine folate and sodium fluoride as effective ingredients. There is a problem that can happen. Therefore, it is necessary to develop a natural substance which effectively suppresses bad breath and has no side effects.

Pinus pinus Koraiensis , also known as sea urchin, belongs to Pinaceae . Pine nut seeds contain 68.2 to 74% of fat, protein is the most common (16.4%), and other carbohydrate components and various inorganic salts and vitamins of phosphorus are abundantly contained. The leaves of pine trees contain alkaloids such as phymecholine and pyrene. In addition, the pine tree leaves contain a lot of terpenes such as pine needles, and the terpine component is said to have useful functions such as eliminating thrombus and antioxidant action. These leaves contain ascorbic acid and vitamins A, B, and K and bitter tastes, flavonoids, and anthocyanins, and include 7-12% of rosin and 5% of tannins, carbides, and essential oils.

However, it is not yet known whether or not the pine tree has the effect of promoting oral hygiene, preventing and treating cavities, and removing bad breath.

The inventors of the present invention have completed the present invention by studying a new effect and application of pine tree extract in studying to develop a composition for improving oral hygiene and deodorization, and applying it to enhance oral hygiene.

Korean Patent Application No. 10-2007-0012081 (February 06, 2007)

In one aspect, the present invention provides a composition for enhancing oral hygiene derived from natural origin and a method for producing the same.

In one aspect, the present invention provides a composition for enhancing oral hygiene comprising a pine tree extract as an active ingredient.

The present invention also provides, in another aspect, a method of making the composition,

A step of subdividing the pine tree leaves;

Extracting the essential oil through steam distillation;

Cooling and condensing; And

Wherein the composition comprises a dehydrating step.

Although the pine tree extract has no previously reported effect on oral hygiene promotion, in one aspect of the present invention, the composition containing the pine tree extract has remarkably excellent effects in terms of oral anti-microbial activity and bad breath inhibiting ability.

Fig. 1 shows a process for producing gum containing a pine tree essential oil.

In one aspect, the present invention may be a composition for enhancing oral hygiene containing a pine tree extract.

The pine wood of the present invention may be a pine tree leaf in one side.

The pine tree extract of the present invention may contain essential oils by distillation in one aspect.

The component content in the pine tree extract of the present invention may be such that the content of Camphene in one aspect is 10 to 30% by weight (wt%) based on the weight of the total extract. According to one embodiment, the content of camphan is at least 5%, at least 7%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30% 35 wt% or more, or 40 wt% or more, 5 wt% or less, 7 wt% or less, 10 wt% or less, 15 wt% or less, 20 wt% or less, 25 wt% or less, 30 wt% , Or 40 wt% or less. Preferably 15% by weight to 25% by weight, or 19% by weight to 22% by weight, and more preferably 20.85% by weight.

In another aspect, the content of [beta] -myrcene in the components of the pine cones extract is 10 to 25 wt% based on the weight of the total extract and the content of 3-Carene is 3 to 10 wt% 10 to 18 wt%. According to one embodiment, the content of? -Myrcene is at least 5 wt%, at least 7 wt%, at least 10 wt%, at least 13 wt%, at least 16 wt%, at least 19 wt%, at least 22 wt% At least 25 wt%, at least 28 wt%, or at least 30 wt%, or at least 5 wt%, at least 7 wt%, at least 10 wt%, at least 13 wt%, at most 16 wt% Or less, 25 weight% or less, 28 weight% or less, or 30 weight% or less. Preferably, the content of? -Myrcene may be 15 to 19% by weight or 16 to 18% by weight, and more preferably 17.81% by weight, based on the weight of the total extract. According to another embodiment, the content of 3-karen is greater than or equal to 4%, 7%, 10%, 12%, 14%, 16%, 18% Or more, 20 wt% or more, 25 wt% or more, or 30 wt% or more, 4 wt% or less, 7 wt% or less, 10 wt% or less, 12 wt% or less, 14 wt% or less, 16 wt% Up to 20 wt%, up to 25 wt%, or up to 30 wt%. Preferably, the content of 3-carene may be 11 to 15% by weight or 12 to 14% by weight, and more preferably 13.86% by weight, based on the weight of the total extract.

In another aspect, the content of [beta] -pinene in the components of the pine tree extract is 5 to 15 wt% based on the weight of the total extract, and the content of [alpha] -pinene is 5 to 15 wt% 15 wt%. According to one embodiment, the content of? -Pinene is at least 2 wt%, at least 4 wt%, at least 6 wt%, at least 8 wt%, at least 10 wt%, at least 12 wt%, at least 14 wt% Or more, 16 wt% or more, 18 wt% or more, 20 wt% or more, 2 wt% or less, 4 wt% or less, 6 wt% or less, 8 wt% or less, 10 wt% or less, 12 wt% Or less, 16 weight% or less, 18 weight% or less, or 20 weight% or less. Preferably, the content of? -Pinene may be 10 to 12% by weight, more preferably 11.25% by weight, based on the weight of the total extract. According to another embodiment, the content of? -Pinene is at least 2 wt%, at least 4 wt%, at least 6 wt%, at least 8 wt%, at least 10 wt%, at least 12 wt%, at least 14 wt% Or more, 16 wt% or more, 18 wt% or more, 20 wt% or more, 2 wt% or less, 4 wt% or less, 6 wt% or less, 8 wt% or less, 10 wt% or less, 12 wt% Or less, 16 weight% or less, 18 weight% or less, or 20 weight% or less. Preferably, the content of? -Pinene may be 8 to 13% by weight or 9 to 12% by weight, more preferably 10.51% by weight, based on the weight of the total extract.

In another aspect, the content of naphthalene in the components of the pine tree extract may be 5 to 10 wt%, based on the weight of the total extract. According to one embodiment, the content of naphthalene is at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8% At least 9 wt%, at least 10 wt%, at least 11 wt%, at least 12 wt%, or at least 15 wt%, or at least 2 wt%, at least 3 wt%, at least 4 wt%, at least 5 wt% , 7 wt% or less, 8 wt% or less, 9 wt% or less, 10 wt% or less, 11 wt% or less, 12 wt% or less, or 15 wt% or less. Preferably from 5 to 10% by weight, or from 6 to 8% by weight, and more preferably from 7.01% by weight.

In another aspect, the content of (+) - 4-carnen (+) - 4-carnen in the components of the pine tree extract is at least 2% by weight, at least 3% by weight, at least 4% At least 5 wt%, at least 6 wt%, at least 7 wt%, at least 8 wt%, or at least 9 wt%, or at least 2 wt%, at least 3 wt%, at least 4 wt% Or less, 7 weight% or less, 8 weight% or less, or 9 weight% or less. Preferably 2 to 7% by weight or 3 to 6% by weight, and more preferably 4.51% by weight.

In another aspect, the content of? -Caryophyllene in the components of the pine cones extract is at least 0.5 wt%, at least 1 wt%, at least 1.5 wt%, at least 2 wt%, at least 2.5 wt% Not less than 3%, not less than 3.5%, not less than 4%, or not less than 5%, not more than 0.5%, not more than 1%, not more than 1.5%, not more than 2% By weight, up to 3.5% by weight, up to 4% by weight, or up to 5% by weight. Preferably 1 to 4% by weight or 2 to 3% by weight, and more preferably 2.36% by weight.

In another aspect, the content of D-Limonene in the components of the pine cones extract is at least 0.5 wt%, at least 1 wt%, at least 1.5 wt%, at least 2 wt%, at least 2.5 wt% Or more, 3 wt% or more, 3.5 wt% or more, 4 wt% or more, or 5 wt% or more, 0.5 wt% or less, 1 wt% or less, 1.5 wt% or less, 2 wt% or less, 2.5 wt% Or less, 3.5 wt% or less, 4 wt% or less, or 5 wt% or less. Preferably 1 to 4% by weight or 1.5 to 3% by weight, and more preferably 2.1% by weight.

In another aspect, the content of a-phellandrene in the components of the pine cones extract is at least 0.5 wt%, at least 1 wt%, at least 1.5 wt%, at least 2 wt%, at least 2.2 wt% At least 2.5 wt%, at least 3 wt%, at least 3.5 wt%, at least 4 wt%, or at least 5 wt%, or at least 0.5 wt%, at least 1 wt%, at least 1.5 wt% 2.2 wt% or less, 2.5 wt% or less, 3 wt% or less, 3.5 wt% or less, 4 wt% or less, or 5 wt% or less. Preferably 0.5 to 4% by weight or 1 to 3% by weight, and more preferably 2.03% by weight.

In another aspect, the content of bicyclo [4.1.0] heptane in the components of the pine cone extract is 0.3 wt% or more, 0.6 wt% or more, 0.9 wt% or more At least 1.2 wt%, at least 1.5 wt%, at least 1.8 wt%, at least 2.2 wt%, at least 2.5 wt%, at least 2.8 wt%, at least 3.1 wt%, at least 3.5 wt%, at least 4.0 wt% , Or no less than 5.0 wt%, no more than 0.3 wt%, no more than 0.6 wt%, no more than 0.9 wt%, no more than 1.2 wt%, no more than 1.5 wt%, no more than 1.8 wt%, no more than 2.2 wt% Or less, 3.1 wt% or less, 3.5 wt% or less, 4.0 wt% or less, 4.5 wt% or less, or 5.0 wt% or less. Preferably 0.5 to 2.5% by weight or 1.0 to 2.2% by weight, and more preferably 1.84% by weight.

In another aspect, the content of Tricyclo [5.4.0.0. (2,8)] undec-9-ene in the components of the pine tree extract is 0.3 wt% or more, 0.6 wt% or more, 0.9 wt% or more At least 1.2 wt%, at least 1.5 wt%, at least 1.8 wt%, at least 2.2 wt%, at least 2.5 wt%, at least 2.8 wt%, at least 3.1 wt%, at least 3.5 wt%, at least 4.0 wt% , Or no less than 5.0 wt%, no more than 0.3 wt%, no more than 0.6 wt%, no more than 0.9 wt%, no more than 1.2 wt%, no more than 1.5 wt%, no more than 1.8 wt%, no more than 2.2 wt% Or less, 3.1 wt% or less, 3.5 wt% or less, 4.0 wt% or less, 4.5 wt% or less, or 5.0 wt% or less. Preferably 0.5 to 2.5% by weight or 1.0 to 2.2% by weight, and more preferably 1.69% by weight.

As used herein, the term " extract "includes all substances obtained by extracting components of a natural product, regardless of the extraction method, extraction solvent, extracted component or extract form, The present invention relates to a method for producing a fermented product, a method for producing the fermented product, a method for producing the fermented product, a method for producing the fermented product, , &Lt; / RTI >

The hygiene enhancement of the present invention may include, in one aspect, antibacterial or deodorant.

In one aspect of the invention, the antimicrobial is a Lactobacillus bacteria (Lactobacillus) genus, Staphylococcus (Staphylococcus) genus, Enterobacter nose kusu (Enterococcus) genus, and at least one selected from the group consisting of genus Streptococcus (Streptococcus) Or may be antimicrobial against any one or more of the bacteria or cavities in the mouth corresponding to the genus.

In another aspect of the present invention, the oral cavity bacterium or caviphile is selected from the group consisting of Lactobacillus paracasei , Staphylococcus aureus , Enterococcus faecalis , Streptococcus mutans Streptococcus mutans , Streptococcus mutans , Streptococcus sobrinus , and the like.

The composition of the present invention may comprise food in one aspect and gum in another aspect.

In the food composition according to one aspect of the present invention, the kind of the food is not particularly limited. Examples of the food to which the composition of the present invention can be added include dairy products including dairy products such as gum, drink, meat, sausage, bread, biscuits, rice cakes, candies, snacks, confectionery, pizza, ramen noodles, , Beverages, alcoholic beverages, and vitamin complexes, and includes common foods, health foods, supplements, health functional foods, and various food additives in a conventional sense.

The composition according to one aspect of the present invention may contain other components or the like which can give a synergistic effect to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.001 to 10% by weight, more specifically 0.001 to 5% by weight, based on the total weight of the composition.

The composition of the composition according to one aspect of the present invention is not particularly limited, but may be, for example, a liquid, such as gum, chewing gum, solid, semi-solid, tablet, granule, powder, drink, emulsion, Gel, bar, and the like. The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients. It may also be administered in a variety of ways such as chewing (mastication, ingestion and chewing), oral administration, simple drinking, injection administration, spraying, squeezing, or a combination thereof.

In addition to the above, the food composition which is one aspect of the present invention may be in the form of flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and enhancers, pectic acid and its salts, alginic acid and its salts, , Protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the functional food compositions of the present disclosure may include natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally comprised in the range of 0 to about 40 parts by weight per 100 parts by weight of the composition herein.

In one aspect, the extract of the present invention may be 0.0001 to 10 wt%, and may be 0.0001 to 1 wt%, based on the total weight of the composition. If the extract is less than 0.0001 wt%, the above-mentioned various effects are insignificant. If the amount of the extract is more than 10 wt%, the effect ratio of the other ingredients is affected without increasing the apparent effect due to the increase of the content. In another aspect, the extract of the present invention has an activity of at least 0.0001, at least 0.001, at least 0.005, at least 0.01, at least 0.015, at least 0.02, at least 0.023, at least 0.026, at least 0.03, at least 0.032, at least 0.035, at least 0.038 , At least 0.041, at least 0.05, at least 0.06, at least 0.07, at least 0.08, at least 0.09, at least 0.1, at least 0.2, at least 0.3, at least 0.4, at least 0.5, at least 0.6, at least 0.7, at least 0.8, at least 0.9, 1.2 or more, 1.3 or more, 1.4 or more, 1.5 or more, 1.6 or more, 1.7 or more, 1.8 or more, or 1.9 or more. (Wt%) selected from the group consisting of at least 2.0, at least 3.0, at least 4.0, at least 5.0, at least 6.0, at least 7.0, at least 8.0, at least 9.0 and at most 10.0, To 1 wt%, or 0.02 to 0.03 wt%, depending on various factors such as the age, health condition, and complications of the subject.

In one aspect, the composition of the present invention may contain one or more wt% selected from the group consisting of not less than 5, not less than 15, not less than 25, not less than 35, and not more than 45 when the gum base is compared to the total composition weight , Preferably from 15 to 25 wt%.

In one aspect, the composition of the present invention contains at least one wt% of xylitol selected from the group consisting of 5 or more, 15 or more, 25 or more, 35 or more, 45 or more, 55 or more, , And preferably 25 to 35 wt%.

In one aspect, the invention provides a method of making the composition,

A step of subdividing the pine tree leaves;

Extracting the essential oil through steam distillation;

Cooling and condensing; And

And a dehydrating step.

Extraction can be performed, for example, by distillation extraction, steam distillation extraction, hot water extraction, heat extraction, cold extraction, reflux extraction, reflux cooling extraction or ultrasonic extraction. none. Extraction may be carried out at room temperature, but it may be carried out under heating at a temperature of about 40 to 300 DEG C, more preferably about 70 to 200 DEG C for more efficient extraction, It is not. The extraction time is preferably about 2 to 8 hours, and more preferably about 3 to 6 hours. However, the extraction time may vary depending on conditions such as extraction solvent and extraction temperature, The reaction can be carried out at 90 to 100 ° C in the case of water and at 65 to 70 ° C in the case where the solvent is ethanol. The extraction can be performed one or more times several times to obtain a larger amount of the active ingredient, preferably 1 to 8 times, more preferably 3 to 6 times, and the combined extract can be used. Specifically, in one aspect of the present invention, the extraction may be a reflux extraction and may be performed at room temperature.

In another aspect of the present invention, the extraction may be distillation extraction, and the distillation may be applied to any kind of distillation, distillation, fractionation, azeotropic distillation, steam distillation and the like, more preferably steam distillation And may be steam distillation.

The method of the present invention, in one aspect, comprises the step of blending a product comprising the product produced by the process and a gum base; An extrusion step; Molding step; Separation step; And a coating step.

In another aspect, the method of the present invention may further comprise a cooling step, an aging step and a core separation step after the molding step.

The coating of the present invention may in one aspect be through a white coating followed by a dye coating.

In one aspect of the invention, the composition may be a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, soft capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical dosages are 0.0001 mg / kg / day to 2000 mg / kg / day, more specifically 0.0005 mg / kg / day to 1500 mg / kg / day.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It should be apparent to those skilled in the art that these examples and experiments are for the purpose of illustrating the present invention only and that the scope of the present invention is not construed as being limited by these examples and experimental examples .

[ Example 1] Extract of essential oil from pine tree leaf

Pine wood steam distillation method. First, the pine tree leaves were cut into a steam distillation apparatus, and the oil was extracted by generating steam at a high temperature. Then, the crude oil extract condensed through a cooling pipe was separated from water and dehydrated to obtain a pine leaf essential oil.

[ Example 2] Analysis of Components of Essential Oil of Pine Needle

The analysis of the components of the obtained pine leaf essential oil was performed using GC / MS HP-7890A / 5975C (Agilent Co., USA). The column was equipped with HP-5MS (30 mx25 mx 0.25 m, Agilent Technologies, USA), and the temperature was maintained at 50 DEG C for 5 minutes, then the temperature was increased by 5 DEG C per minute to 220 DEG C, For 20 minutes. The temperature of the injection port was set at 250 ° C. and He gas (1 mL / min) was used as the carrier gas. The identity of the compounds was determined by GC-MS mass spectrometry using a Wiley 275L database. The major components are shown in the table below.

division compound R.T (min) Content (%) One Camphene 10.58 20.85 2 β-Myrcene 13.87 17.81 3 3-Carene 15.28 13.86 4 β-Pinene 12.18 11.25 5 α-Pinene 9.77 10.51 6 Naphthalene 108.89 7.01 7 (+) - 4-Carnen 15.97 4.51 8 alpha-caryophyllene 94.54 2.36 9 D-Limonene 17.62 2.10 10 α-Phellandrene 14.69 2.03 11 Bicyclo [4.1.0] heptane 213.31 1.84 12 Tricyclo [5.4.0.0 (2,8)] undec-9-ene 71.58 1.69

[ Manufacturing example 1] Raw material mixing ratio of gum containing pine tree essential oil

Pomegranate leaf essential oil containing gum was prepared according to the following ratio. For the sensory evaluation, gum was prepared according to the amount of essential oil and control.

Raw material name Formulation ratio (%) Control Production Example 1 Gum Base 23.6 22.4 Acesulfame K 0.07 0.07 Xylitol 10 10 Isomalt 9 9 Aspartame 0.6 0.6 Hydrogenated Glucose Syrup 8 8 L-menthol (L-Menthol) 0.5 0.5 Food additive mixture (flavoring agent) 1.7 1.7 Liquid Menthol 0.5 0.5 Maltitol 40 40 Gum Arabic 5 6 D-sorbitol (D-sorbitol) 0.6 0.6 Food additive mixture (coloring) 0.2 0.2 Carnauba Wax 0.03 0.03 Shellac 0.2 0.2 Pine tree leaf oil - 0.2 Sum 100 100

[ Experimental Example 1] Sensory Evaluation of Gum Containing Pine Needle Essential Oil

The sensory evaluation was performed by randomly extracting 10 men and women from 20 to 50 years old according to a 5 point rating system (5 = very good, 4 = moderate, 3 = not good or 2 = dissatisfied, 1 = very disliked) As a result of sensory evaluation, as shown in Table 3, the type 1 product showed high preference.

division flavor color incense Texture Comprehensive likelihood Control 3.8 3.8 3.7 3.8 3.8 Production Example 1 4.2 3.8 4.1 3.8 4.2

[ Experimental Example 2] Pine tree leaf essential oil Deodorizing force evaluation

The evaluation of the deodorizing power of pine needles oil was carried out by using the standard test method of ASTM D1988-06 (4) for the representative odor substances such as ammonia (Ammonia), formaldehyde, trimethylamine, and methylmercaptan Standard test method, ASTM D1988-06., 2011), and the green tea extract obtained in the same manner as in Example 1 was used as a control.

A rubber stopper was inserted into a 500 mL Erlenmeyer flask, and a malodorous test liquid was poured into the flask and a malodor was generated. After 30 minutes from the start of the odor, the initial concentration was adjusted, and the same amount of essential oil was injected into the flask. Then, the residual odor gas in the flask was measured by a gas detector (GV-100S, Gastec, Japan). All experiments were repeated 3 times.

As a result, the essential oil of P. vannamei showed deodorizing effect on all four kinds of odorous substances and showed a high deodorizing power against ammonia relative to other odorous substances. The deodorizing power of ammonia increased to 43.3% after 10 minutes from the injection of the essential oil of Pinus koraiensis. It increased gradually with time and reached 90.7% after one hour, 69.2% Showed high deodorizing effect. Deodorizing effect on trimethylamine also showed high deodorizing power over time and 20.3% higher deodorizing power than the control. It was confirmed that the deodorizing power of formaldehyde was lower than that of other odor materials. The deodorizing power for methyl mercaptan was 59.7% for the initial 10 minutes, and the deodorizing power was increased with time, indicating that the deodorizing power was 88.9% after 1 hour.

Test substance Odor substance Deodorizing force (%) 10 minutes 20 minutes 30 minutes 60 minutes Pine tree leaf oil ammonia 43.3 ± 4.5 73.7 ± 4.9 79.5 ± 5.1 90.7 ± 5.2 Trimethylamine 34.1 ± 3.2 53.8 ± 3.4 56.7 ± 1.2 65.5 ± 3.6 Formaldehyde 20.0 ± 1.4 31.6 ± 1.6 36.8 ± 1.8 39.5 ± 1.1 Methyl mercaptan 59.7 ± 1.1 75.0 ± 1.5 79.3 ± 1.4 88.9 ± 1.3 Green tea extract ammonia 15.2 ± 2.3 16.8 ± 2.8 20.2 ± 1.5 21.5 ± 3.8 Trimethylamine 25.8 ± 1.6 36.4 ± 2.5 40.1 ± 4.8 45.2 ± 5.2 Formaldehyde 0.0 ± 0.0 0.0 ± 0.0 4.5 ± 1.2 4.8 ± 1.5 Methyl mercaptan 5.4 ± 0.5 15.2 ± 2.1 24.1 ± 1.4 28.1 ± 1.3

[ Experimental Example 3] Evaluation of Antimicrobial Activity of Essential Oil of Pine Needle

Three kinds of oral bacteria and two kinds of cavities were used for the antibacterial activity test. Oral bacterium Lactobacillus paracasei (ATCC 25302), Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212) was used as received from ATCC received pre-sale pre-sale at the (American Type Culture Collection), chungchigyun of Streptococcus mutans (KCTC 3065), Streptococcus sobrinus (KCTC 3288) notes that Korea Life Research Biological Resource Center (BRC).

First L. Lactobacilli MRS broth (MRS), S. aureus (ATCC 25923), Trypticase Soy Broth (TSB), and paracasei (ATCC 25302) faecalis (ATCC 29212) was Trypticase Soy Broth (TSB), S. mutans (KCTC 3065) and S. sobrinus (KCTC 3288) containing a 5% sheep blood was preincubated with a Luria bertani media Broth (LB) medium . The pre-cultured strains were again plated on a plate medium. L. paracasei (ATCC 25302), MRS, S. aureus (ATCC 25923), Trypticase Soy Agar (TSA) and E. coli. Faecalis (ATCC 29212) was cultured in Trypticase Soy Agar (TSA) medium containing 5% sheep blood at 37 ℃ under aerobic conditions. S. mutans (KCTC 3065) and S. sobrinus (KCTC 3288) were cultured in anaerobic conditions at 37 ℃ using Brain Heart Infusion (BHI) medium.

The cultured strain was used to evaluate the antibacterial activity.

Specifically, first, the test strain was activated by subculturing three times in slope medium, and then inoculated aseptically into a liquid medium for each strain for 24 hours in a shaking incubator (WIS-10R wisecube) And the suspension was suspended in sterile distilled water to have an absorbance of 0.1 (600 nm). Antimicrobial activity was measured by disc diffusion method using paper disc (diameter 8 mm) (Piddock., 1990). The culture broth was sterilized by dispensing 15 mL of medium in Petri dish, and 100 μL of each culture was sterilized using a sterilized smear rod. The sterilized paper disc was placed on this medium, The filter was sterilized with 25 μL of essential oil and cultured at 37 ° C for 24 hours. The clear zone (mm) formed around the disc was photographed using a microscope (Leica KL 200) and measured using the Leica Application suite program.

As a result, the antimicrobial activity of L. paracasei, S. aureus, E. faecalis, S. mutans, and S. sobrinus was evaluated for the antibacterial activity of P. vannamei essential oil, and a general propolis extract sold in the market was used as a control . 25 μL of each sample was absorbed into paper discs to measure inhibition of growth on oral bacteria. The results showed that the essential oil of Pinus koraiensis showed antimicrobial activity against all bacteria except E. faecalis. The highest antimicrobial activity against S. aureus was 7.59mm, and the antimicrobial activity against S. aureus was higher than that of the control propolis extract. The antimicrobial activity against L. paracasei was about 1.28mm higher than that of the control, while the antimicrobial activity against S. aureus and S. sobrinus was slightly lower than that of the control.

sample
(25 [mu] L) Clear zone (mm) L. paracasei S. aureus E. faecalis S. mutans S. sobrinus Pinus koraiensis
essential oil 3.15 ± 0.18 3.27 ± 0.11 - 7.59 ± 0.19 2.08 ± 0.15 Propolis extract 1.87 ± 0.15 4.52 + - 0.21 1.25 + 0.09 5.36 + 0.17 2.58 ± 0.12

Claims

A composition for enhancing oral hygiene comprising a pine tree extract as an active ingredient.

The method according to claim 1,
Wherein the pine wood is a pine tree leaf.

The method according to claim 1,
Wherein the extract comprises an essential oil by a distillation method.

The method according to claim 1,
Wherein the extract has a content of Camphene of 10 to 30% by weight (wt%) based on the weight of the total extract.

The method according to claim 1,
The extract is characterized in that the content of? -Myrcene is 10 to 25 wt% based on the weight of the total extract and the content of 3-Carene is 10 to 18 wt% / RTI > composition for enhancing oral hygiene.

The method according to claim 1,
Wherein the extract has an amount of β-pinene of 5 to 15 wt% based on the weight of the total extract and a content of α-pinene of 5 to 15 wt% of the total extract, / RTI >

The method according to claim 1,
Wherein the hygiene enhancement includes antibacterial or deodorant.

The method according to claim 1,
Wherein the composition comprises a food product.

The method according to claim 1,
Wherein the composition comprises gum.

The method according to claim 1,
Wherein the extract is 0.0001 to 1 wt% based on the total weight of the composition.

The method according to claim 1,
Wherein the extract is 0.02 to 0.03 wt% based on the total weight of the composition.

13. A process for preparing a composition according to any one of claims 1 to 11,
A step of subdividing the pine tree leaves;
Extracting the essential oil through steam distillation;
Cooling and condensing; And
And a dehydrating step.

13. The method of claim 12,
Said method comprising the steps of blending a resultant produced by the method of claim 12 with a raw material comprising a gum base; An extrusion step; Molding step; Separation step; And a coating step.

14. The method of claim 13,
The method further comprises a cooling step after the forming step, an aging step and a core separating step.

14. The method of claim 13,
Wherein the coating is subjected to a white coating followed by a dye coating.

The method of claim 7, wherein the antibiotic is a Lactobacillus bacteria (Lactobacillus) genus, Staphylococcus (Staphylococcus) genus, Enterobacter nose kusu (Enterococcus) genus, and Streptococcus in the one or more selected from the group consisting of genus (Streptococcus) Wherein the composition is antibacterial against any one or more of the corresponding bacteria or cucurbit bacteria in the oral cavity.

17. The oral cavity bacterium according to claim 16, wherein the bacterium or caviphilid in the oral cavity is selected from the group consisting of Lactobacillus paracasei , Staphylococcus aureus , Enterococcus faecalis , Streptococcus mutans Streptococcus mutans ), and Streptococcus sobrinus . The composition for promoting oral hygiene of the present invention is a composition for enhancing oral hygiene.