KR20200106371A - Anti-microbial composition comprising pinus koraiensis sieb extract - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising a Pinus koraiensis SIEB leaf extract, a pharmaceutical composition comprising the antibacterial composition, a food composition, and a cosmetic composition.

Description

Antimicrobial composition containing pine needle extract {ANTI-MICROBIAL COMPOSITION COMPRISING PINUS KORAIENSIS SIEB EXTRACT}

The present invention relates to an antibacterial composition comprising a pine tree ( Pinus koraiensis SIEB) leaf extract, a pharmaceutical composition comprising the antimicrobial composition, a food composition, and a cosmetic composition.

Pinus koraiensis SIEB, also known as hongsong (紅松), has habitats in northeastern China and Japan, including Korea, and grows above 1,000m above sea level. It is known that the amount of phytoncide is less than other coniferous trees, but is not toxic to the human body at all.In particular, the phytoncide of the pine tree has the highest content of alpha-pinene, which stimulates the cerebral cortex to increase concentration and exercise, and 3 others. -It contains Karen, Bonil-Acetate, etc., showing the unique effect of forest fragrance.

The causes of vaginitis vary from bacteria, viral infections, fungi, long-term use of antibiotics or contraceptives, and excessive use of vaginal cleansers. Vaginitis occurs through the process of proliferating opportunistic pathogens as the balance of the vaginal flora is broken. It can develop.

Vaginitis is largely divided into Candida vaginitis, Trichomonas vaginitis, and bacterial vaginosis. Candida vaginitis is the most common form of vaginitis caused by the fungus Candida albicans . About 75% of women suffer from Candida vaginitis of the vagina and vulva more than once during their lives, and 45% of women have a year. It is so common that more than two recurrences occur. The major pathogen of bacterial vaginosis is Gardnerella vaginalis , and the weakly acidic vaginal environment maintained by lactic acid bacteria is destroyed, and Gardnerella vaginalis proliferates, causing bacterial vaginosis.

Currently, many studies have been conducted on vaginitis treatments and treatment methods to prevent and treat vaginitis, and it is common to use a method of oral administration of a vaginitis drug such as metronidazole or antibiotics in the form of a cream. However, in the case of conventional treatments and treatment methods, the cure rate is only about 60 to 70% at 1 month after treatment, and about 30% of cases in which metronidazole has been effective in recurrence within 3 months. It has been reported to be. In addition, 83% of lactobacilli were present in the vagina immediately after antibiotic treatment, but only 65% remained after 1 month of treatment. This disease is highly likely to recur.

Korean Patent Registration No. 10-1652700 Korean Patent Publication No. 10-2004-0027240

It is an object of the present invention to provide an antibacterial composition against yeast infection-causing bacteria, comprising a leaf extract of Pinus koraiensis SIEB.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating vaginitis comprising the antibacterial composition.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating vaginitis comprising the pine tree leaf extract.

Another object of the present invention is to provide a method for treating vaginitis, comprising administering the pine needle leaf extract to an individual.

Another object of the present invention is to provide a food composition for preventing or improving vaginitis comprising the pine tree leaf extract.

Another object of the present invention is to provide a cosmetic composition for preventing or improving vaginitis comprising the pine tree leaf extract.

One aspect of the present invention for achieving the above object provides an antibacterial composition against yeast infection-causing bacteria, including a leaf extract of Pinus koraiensis SIEB. The vaginitis causative bacteria are Gardnerella vaginalis, Candida albicans, and It may be one or more selected from the group consisting of Staphylococcus aureus .

The pine tree leaf extract is obtained from Pinus koraiensis SIEB, and the pine tree leaves include essential oil components such as terpene, phenolics, and tannin, chlorophyll components, inorganic and organic components, and vitamins. It is known to contain various ingredients.

The "extract" of the present invention refers to the extract obtained by the extraction treatment of the pine tree leaves, the diluent or concentrate of the extract, the dried product obtained by drying the extract, the preparation or purified product of the extract, or a mixture thereof, the extract itself And extracts of all formulations that can be formed using the extract.

The extract of the present invention may be extracted from natural, hybrid or variant plants, and may be extracted from plant tissue culture.

The extract may be extracted with water, alcohol, ethyl acetate, acetone, nucleic acid, dichloromethane, or a mixed solvent thereof.

In addition, the extract can be obtained by extraction and filtration by conventional methods such as hot water extraction, ultrasonic extraction, pressurized extraction, etc., and specifically, it can be obtained by steam distillation extraction method.

The steam distillation extraction method is well known in the art, and the ratio of the pine tree leaves and purified water for performing the steam distillation extraction is 1:1 to 1:10, specifically 1:1 to 1:3, more specifically 1 : 2 (by weight). The pine needles and purified water are distilled once or multiple times in a distillation tank at 70 to 500°C, specifically 80 to 150°C, more specifically 90°C, for about 1 hour to several days, specifically 3 to 4 hours. . The condensate obtained by cooling and condensing the distillate thus obtained can be used in the present invention. Further, the condensate thus obtained may be further concentrated and filtered by a conventional method in the art to obtain a higher purity pine tree leaf extract. By the steam distillation extraction method, the pine tree leaf extract can be obtained in the form of a clear, colorless and transparent oil (oil) with a pine tree scent (distilled extraction oil).

The pine tree leaf extract has antibacterial activity against Gardnerella vaginalis, Candida albicans, and Staphylococcus aureus, which are major causative bacteria of vaginitis. Leaf extract can be used as a composition for preventing or treating vaginitis by inhibiting or killing harmful bacteria in the vagina.

In an embodiment of the present invention, it was confirmed that the antibacterial activity was excellent against the main causative bacteria of vaginitis , Gardnerella parangalis , Candida albicans , and Staphylococcus aureus (Table 1). .

Another aspect of the present invention provides a pharmaceutical composition for the treatment or prevention of vaginitis comprising the pine tree leaf extract.

The “prevention” refers to any action that suppresses vaginitis or delays the onset of vaginitis by administration of the pharmaceutical composition according to the present invention.

The “treatment” refers to any action in which symptoms of vaginitis are improved or beneficially changed by administration of the pharmaceutical composition according to the present invention.

The pharmaceutical composition may include a pharmaceutically acceptable carrier in addition to the active ingredient. At this time, the pharmaceutically acceptable carrier is commonly used in the formulation, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose , Polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil, but are not limited thereto. In addition, a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like may be additionally included in addition to the above components.

The pharmaceutical composition may be administered orally or parenterally according to a desired method (for example, intravenously, subcutaneously, intraperitoneally or topically), and the dosage may be the condition and weight of the patient, the degree of disease, It depends on the drug form, administration route and time, but may be appropriately selected by those skilled in the art.

The pharmaceutical composition is administered in a pharmaceutically effective amount. The “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, and drug Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field.

The pharmaceutical composition may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, which can be easily determined by a person skilled in the art.

Another aspect of the present invention provides a method for treating vaginitis comprising administering the pharmaceutical composition to a subject.

The "individual" refers to a subject in need of treatment of a disease, and more specifically, refers to a mammal such as a human or non-human primate, mouse, dog, cat, horse and cow.

Specifically, the effective amount of the pharmaceutical composition may vary depending on the patient's age, sex, condition, body weight, absorption of the active ingredient in the body, the inactivation rate and excretion rate, the type of disease, and the drug to be used in combination. 0.001 to 150 mg per sugar, preferably 0.01 to 100 mg, may be administered daily or every other day, or divided into 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, the severity of obesity, sex, weight, age, etc., the dosage amount does not limit the scope of the present invention in any way.

Another aspect of the present invention provides a food composition for preventing or improving vaginitis comprising a pine tree leaf extract.

The "improvement" means any action that at least reduces the severity of a parameter related to the condition being treated, for example a symptom.

The food may exemplify meat, snacks, dairy products, beverages, and the like, but is not limited thereto, and may be understood as a concept including all conventional functional foods.

The food composition may contain ordinary food additives, and the food additives are subject to the standards and standards for the relevant item according to the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. Whether it is appropriate or not can be judged.

The items described in the above food additive code include, for example, ketones, glycine, potassium citrate, nicotinic acid, cinnamic acid, and other chemical compounds, red pigment, licorice extract, crystalline cellulose, high color pigments, natural additives such as guar gum, sodium L-glutamate preparations, and noodles. Mixed preparations, such as an added alkali agent, a preservative preparation, and a tar color preparation, are mentioned.

The food composition may be variously used in foods and beverages for improving vaginitis, and for example, various foods, beverages, gum, tea, vitamin complexes, health functional supplements, food additives, and the like.

The food composition may include 0.001 to 80.0% by weight of the composition based on the total weight for the purpose of improving vaginitis. If the composition is less than 0.001% by weight, the effect of improving vaginitis may not be sufficiently realized, and if it exceeds 80.0% by weight, the original product quality may not be realized or cost efficiency may be lowered.

The food composition may be prepared and processed into any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills for the purpose of improving vaginitis.

Specifically, the functional food in the form of tablets is a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, and other additives are granulated by a conventional method, and then compression molded by putting a lubricant or the like, or the mixture is It can be produced by direct compression molding. In addition, the functional food in the form of a tablet may contain a mating agent or the like as necessary, and may be coated with a suitable coating agent if necessary.

Among the capsule-type food compositions, hard capsules can be prepared by filling a conventional hard capsule with a mixture of additives such as eggplant extracts and excipients, or granules or skinned granules thereof, and soft capsules are wood extracts or fractions thereof. , And a mixture of additives such as excipients can be prepared by filling a capsule base such as gelatin. The soft capsules may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, and the like, if necessary.

The ring-shaped food composition may be prepared by molding a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, etc. in an appropriate way, and if necessary, a coating agent, or starch, talc Alternatively, you can make a ring with a suitable substance.

The granular food composition may be prepared in a granular form of a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, etc., and may contain flavoring agents, flavoring agents, etc., if necessary.

In addition, the definitions of terms for the excipients, binders, disintegrants, lubricants, flavoring agents, flavoring agents, and the like are described in documents known in the art, and may include those having the same or similar functions.

Another aspect of the present invention provides a cosmetic composition for improving or preventing vaginitis comprising a pine needle extract.

The cosmetic composition includes softening lotion, nutritional lotion, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, shampoo, conditioner, pack, It may be formulated as one or more selected from the group consisting of spray and powder.

The cosmetic composition may be formulated by a conventional method. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the content disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions. , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial lotion and body lotion; Creams such as nourishing cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Alternatively, it may be formulated with a detergent such as cleansing foam, soap, and body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In the cosmetic composition, in addition to the essential ingredients in each formulation, other ingredients may be appropriately blended within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use.

The cosmetic composition may contain a generally acceptable carrier, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or their Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. Can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

The present invention is a vaginitis causative bacteria Gardnerella vaginalis (Gardnerella vaginalis), Candida albicans (Candida albicans) and It relates to a pine tree leaf extract having antibacterial activity against Staphylococcus aureus, and a composition for treating and preventing vaginitis comprising the same, wherein the natural product, pine leaf extract, has low toxicity to the human body, and against the causative bacteria. Since the antibacterial effect is excellent, it can be usefully used in the prevention, improvement or treatment of vaginitis.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Preparation of pine leaf extract

Fresh pine pine leaves (Gapyeong-gun, Gyeonggi-do, Hongcheon-gun, Gangwon-do) were collected, diseased, insect-eaten, and dry ones were selected and removed, and the selected pine needles were placed in a washing tank and washed three times with running water.

The washed pine needles were dried in the shade for 3 days, and they were regularly cut into about 10 mm using a cutting machine. The cut pine trees were transferred to a distillation tank.

After putting the pine tree leaves and purified water in the distillation tank at a ratio of 1:1, the extraction temperature of the pine trees leaves of the distillation tank was maintained at 90°C and distilled for 3 to 4 hours. At this time, the temperature of the cooling water supplied to the inside of the cooler was maintained at about 18°C, and the condensate passed through the cooler was collected in a condensate separator.

Since the collected condensate was mixed with oil and water, it was further concentrated and filtered to obtain a high-purity extract. The obtained extract was a clear, colorless and transparent oil that exhibited a pine scent. The final extract obtained by the above extraction process was aged for about 30 days for the balance (stabilization) and flavor and aroma of the final chemical components in a cool and dark place.

Example 2. Confirmation of antibacterial activity against vaginitis causative bacteria

The antimicrobial activity of the pine needle leaf extract extracted from the pine needle leaf was confirmed using the disk diffusion method. Gardnerella vaginalis, Candida albicans, and Staphylococcus aureus, which are the cause of vaginitis, were used as target strains, and activated by subculture at least two times in each optimal medium. Prepared.

Dilute the bacterial suspension with sterile water to have a turbidity of 0.5 (about 10 ⁸ CFU/mL) for Stephylococcus aureus and 1.0 for Candida albicans, take 50 μL, and add 5 mL of top agar (add 0.75% agar). Was inoculated and dispensed on a plate medium (Sabouraud Dextrose Agar) for measuring the disk diffusion method. A sterilized paper disc (8mm, Whatman) was placed on it, and then 20 μL of pine needle leaf extract was dispensed.

At this time, in the case of Gardnerella vaginalis, since Blood Agar Plate was used, the experiment was conducted by spread plate culture. The turbidity of the bacterial suspension was adjusted to 1.0, and then 100 μL was dispensed on a plate medium, smeared with a smear, and 20 uL of the white pine leaf extract was dispensed.

Samples dispensed with pine needles leaf extract into the suspension for each strain were cultured in an incubator, and the diameter (mm) of the inhibitory ring was measured to measure the antibacterial activity and are shown in Table 1.

Strain White pine leaf extract Pine nut extract Pine needle extract Gardnerella Vaginalis
(Gardnerella vaginalis) 13 mm 13 mm 10 mm Candida albicans
(Candida albicans) 15 mm - - Staphylococcus aureus
(Staphylococcus aureus) 14 mm 12 mm 13 mm

As shown in Table 1, it was confirmed that the pine needle leaf extract had a larger inhibitory range than the extracts of similar species such as pine pine or pine needles. In addition, for fungi such as Candida albicans, it was confirmed that only the pine tree leaf extract has an inhibitory effect, and it was confirmed that the pine tree leaf extract of the present invention has antibacterial activity against all three causative bacteria of vaginitis at the same time. Furthermore, it was confirmed that it can be used for the treatment and prevention of vaginitis by inhibiting the causative agent of vaginitis as it exhibits antibacterial activity against all three causative bacteria of vaginitis.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be interpreted as being included in the scope of the present invention.

Claims (7)

Containing Pinus koraiensis SIEB leaf extract,
Gardnerella vaginalis, Candida albicans and Antimicrobial composition for one or more selected from the group consisting of Staphylococcus aureus . The method of claim 1,
The extract is water, alcohol, ethyl acetate, acetone, nucleic acid, dichloromethane, or an antimicrobial composition extracted with a mixed solvent thereof. The method of claim 1,
The pine tree leaf extract is an antibacterial composition obtained by a steam distillation extraction method. The method of claim 1,
The pine tree leaf extract is in the form of an oil, antibacterial composition. Pine tree ( Pinus koraiensis SIEB) a pharmaceutical composition for the prevention or treatment of vaginitis containing leaf extract. Pine tree ( Pinus koraiensis SIEB) vaginitis improvement or prevention food composition comprising a leaf extract. Pine tree ( Pinus koraiensis SIEB) cosmetic composition for improving or preventing vaginitis containing leaf extract.