KR20200106371A - Anti-microbial composition comprising pinus koraiensis sieb extract - Google Patents
Anti-microbial composition comprising pinus koraiensis sieb extract Download PDFInfo
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- KR20200106371A KR20200106371A KR1020190024821A KR20190024821A KR20200106371A KR 20200106371 A KR20200106371 A KR 20200106371A KR 1020190024821 A KR1020190024821 A KR 1020190024821A KR 20190024821 A KR20190024821 A KR 20190024821A KR 20200106371 A KR20200106371 A KR 20200106371A
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- composition
- vaginitis
- leaf extract
- extract
- pine
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- Cosmetics (AREA)
Abstract
Description
본 발명은 잣나무(Pinus koraiensis SIEB) 잎 추출물을 포함하는 항균 조성물, 기 항균 조성물을 포함하는 약학적 조성물, 식품 조성물 및 화장료 조성물에 관한 것이다.The present invention relates to an antibacterial composition comprising a pine tree ( Pinus koraiensis SIEB) leaf extract, a pharmaceutical composition comprising the antimicrobial composition, a food composition, and a cosmetic composition.
잣나무(Pinus koraiensis SIEB)는 홍송(紅松)이라고도 하며 한국을 포함하여 일본, 중국 북동부에 서식지가 분포하고, 해발고도 1,000m 이상에서 자란다. 잣나무는 여타 침엽수에 비해 피톤치드 발생양이 적은 대신 인체에 대한 독성이 전혀 없는 것으로 알려졌으며, 특히 잣나무의 피톤치드에는 대뇌피질을 자극하여 집중력과 운동량을 증가시키는 알파-피넨의 함량이 가장 많고, 그외 3-카렌, 보닐-아세테이트 등이 들어 있어 산림향 특유의 효능을 나타낸다. Pinus koraiensis SIEB, also known as hongsong (紅松), has habitats in northeastern China and Japan, including Korea, and grows above 1,000m above sea level. It is known that the amount of phytoncide is less than other coniferous trees, but is not toxic to the human body at all.In particular, the phytoncide of the pine tree has the highest content of alpha-pinene, which stimulates the cerebral cortex to increase concentration and exercise, and 3 others. -It contains Karen, Bonil-Acetate, etc., showing the unique effect of forest fragrance.
질염의 원인은 세균, 바이러스감염, 곰팡이균, 항생제나 피임약의 장기 복용, 과다한 질 세정제 사용 등으로 다양하며, 질 내 세균총의 평형이 깨지면서 기회감염성 병원체(opportunistic pathogen)가 증식하는 과정을 통해 질염이 발병할 수 있다.The causes of vaginitis vary from bacteria, viral infections, fungi, long-term use of antibiotics or contraceptives, and excessive use of vaginal cleansers. Vaginitis occurs through the process of proliferating opportunistic pathogens as the balance of the vaginal flora is broken. It can develop.
질염은 크게 칸디다성 질염(Candida vaginitis), 트리코모나스성 질염(Trichomonas vaginitis), 세균성 질염(bacterial vaginosis)으로 구분된다. 칸디다성 질염은 곰팡이균인 칸디다 알비칸스(Candida albicans)에 의한 가장 흔한 형태의 질염으로, 약 75%의 여성이 살아가는 동안 한 번 이상 질과 외음부의 칸디다성 질염을 겪으며 45%의 여성이 1년에 2회 이상 재발을 겪을 정도로 흔하게 발병한다. 세균성 질염의 주요 병원체는 가드네렐라 바지날리스(Gardnerella vaginalis)이고, 유산균에 의해 유지되던 약산성의 질 내 환경이 파괴되어 가드네렐라 바지날리스가 증식함으로써 세균성 질염이 발병한다.Vaginitis is largely divided into Candida vaginitis, Trichomonas vaginitis, and bacterial vaginosis. Candida vaginitis is the most common form of vaginitis caused by the fungus Candida albicans . About 75% of women suffer from Candida vaginitis of the vagina and vulva more than once during their lives, and 45% of women have a year. It is so common that more than two recurrences occur. The major pathogen of bacterial vaginosis is Gardnerella vaginalis , and the weakly acidic vaginal environment maintained by lactic acid bacteria is destroyed, and Gardnerella vaginalis proliferates, causing bacterial vaginosis.
현재 질염을 예방하고 치료하기 위한 질염 치료제 및 치료방법이 많이 연구되었으며, 메트로니다졸(Metronidazole)과 같은 질염 치료제를 경구 투여하는 방법 또는 크림 형태의 항생제를 사용하는 것이 일반적이다. 그러나 기존의 치료제 및 치료방법의 경우 치료 후 1개월이 경과한 시점에서 치료율이 60 ~ 70% 정도에 불과할 뿐 아니라, 메트로니다졸 복용으로 치료 효과가 있었던 경우 중 약 30%가 3개월 이내에 질염이 재발한 것으로 보고된 바 있다. 또한, 항생제 치료 직후에는 질 내부에 83%의 유산균이 있었으나 치료 1개월 후에는 단지 65% 만이 잔존하는 등 재발 가능성이 매우 높은 질병이다.Currently, many studies have been conducted on vaginitis treatments and treatment methods to prevent and treat vaginitis, and it is common to use a method of oral administration of a vaginitis drug such as metronidazole or antibiotics in the form of a cream. However, in the case of conventional treatments and treatment methods, the cure rate is only about 60 to 70% at 1 month after treatment, and about 30% of cases in which metronidazole has been effective in recurrence within 3 months. It has been reported to be. In addition, 83% of lactobacilli were present in the vagina immediately after antibiotic treatment, but only 65% remained after 1 month of treatment. This disease is highly likely to recur.
본 발명의 목적은 잣나무(Pinus koraiensis SIEB) 잎 추출물을 포함하는, 질염 원인균에 대한 항균 조성물을 제공하는 것이다. It is an object of the present invention to provide an antibacterial composition against yeast infection-causing bacteria, comprising a leaf extract of Pinus koraiensis SIEB.
본 발명의 다른 목적은 상기 항균 조성물을 포함하는 질염 예방 또는 치료용 약학적 조성물을 제공하는 것이다.Another object of the present invention is to provide a pharmaceutical composition for preventing or treating vaginitis comprising the antibacterial composition.
본 발명의 다른 목적은 상기 잣나무 잎 추출물을 포함하는 질염 예방 또는 치료용 약학적 조성물을 제공하는 것이다.Another object of the present invention is to provide a pharmaceutical composition for preventing or treating vaginitis comprising the pine tree leaf extract.
본 발명의 또 다른 목적은 상기 잣나무 잎 추출물을 개체에 투여하는 단계를 포함하는, 질염 치료방법을 제공하는 것이다.Another object of the present invention is to provide a method for treating vaginitis, comprising administering the pine needle leaf extract to an individual.
본 발명의 또 다른 목적은 상기 잣나무 잎 추출물을 포함하는 질염 예방 또는 개선용 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a food composition for preventing or improving vaginitis comprising the pine tree leaf extract.
본 발명의 또 다른 목적은 상기 잣나무 잎 추출물을 포함하는 질염 예방 또는 개선용 화장료 조성물을 제공하는 것이다.Another object of the present invention is to provide a cosmetic composition for preventing or improving vaginitis comprising the pine tree leaf extract.
상기와 같은 목적을 달성하기 위한 본 발명의 일 측면은 잣나무(Pinus koraiensis SIEB) 잎 추출물을 포함하는, 질염 원인균에 대한 항균 조성물을 제공한다. 상기 질염 원인균은 가드네렐라 바지날리스(Gardnerella vaginalis), 칸디다 알비칸스(Candida albicans) 및 스테필로코커스 아우레우스(Staphylococcus aureus) 로 이루어진 군에서 선택된 하나 이상일 수 있다.One aspect of the present invention for achieving the above object provides an antibacterial composition against yeast infection-causing bacteria, including a leaf extract of Pinus koraiensis SIEB. The vaginitis causative bacteria are Gardnerella vaginalis, Candida albicans, and It may be one or more selected from the group consisting of Staphylococcus aureus .
상기 잣나무 잎 추출물은 잣나무(Pinus koraiensis SIEB)로부터 얻어지며, 잣나무잎은 테르펜(terpene), 페놀화합물(phenolics), 탄닌(tannin) 등의 정유성분과, 엽록소성분, 무기 및 유기성분, 비타민 등의 다양한 성분이 함유되어 있는 것으로 알려져 있다.The pine tree leaf extract is obtained from Pinus koraiensis SIEB, and the pine tree leaves include essential oil components such as terpene, phenolics, and tannin, chlorophyll components, inorganic and organic components, and vitamins. It is known to contain various ingredients.
본 발명의 “추출물”은 상기 잣나무 잎의 추출처리에 의하여 얻어진 추출액, 상기 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다. The "extract" of the present invention refers to the extract obtained by the extraction treatment of the pine tree leaves, the diluent or concentrate of the extract, the dried product obtained by drying the extract, the preparation or purified product of the extract, or a mixture thereof, the extract itself And extracts of all formulations that can be formed using the extract.
본 발명의 상기 추출물은 천연, 잡종 또는 변종 식물로부터 추출될 수 있고, 식물 조직 배양물로부터도 추출이 가능하다.The extract of the present invention may be extracted from natural, hybrid or variant plants, and may be extracted from plant tissue culture.
상기 추출물은 물, 알코올, 에틸아세테이트, 아세톤, 핵산, 디클로로메탄 또는 이들의 혼합 용매로 추출될 수 있다.The extract may be extracted with water, alcohol, ethyl acetate, acetone, nucleic acid, dichloromethane, or a mixed solvent thereof.
또한, 상기 추출물은 열수 추출, 초음파 추출, 가압 추출 등의 통상적인 방법으로 추출 및 여과하여 수득할 수 있으며, 구체적으로 수증기 증류 추출법에 의해 수득할 수 있다.In addition, the extract can be obtained by extraction and filtration by conventional methods such as hot water extraction, ultrasonic extraction, pressurized extraction, etc., and specifically, it can be obtained by steam distillation extraction method.
상기 수증기 증류 추출법은 당 분야에 잘 알려져 있으며, 수증기증류추출을 수행하기 위한 잣나무 잎과 정제수의 비율은 1 : 1 내지 1 : 10, 구체적으로는 1 : 1 내지 1 : 3, 더욱 구체적으로는 1 : 2이다 (중량 기준). 잣나무 잎과 정제수를 증류탱크 내에서 70 내지 500 ℃, 구체적으로는 80 내지 150 ℃, 더욱 구체적으로는 90 ℃에서, 약 1 시간 내지 수일, 구체적으로는 3 내지 4 시간 동안 단회 또는 복수회로 증류시킨다. 이렇게 얻어진 증류물을 냉각 및 응축하여 얻어진 응축물을 본 발명에서 사용할 수 있다. 또한, 이렇게 얻어진 응축물을 당 분야의 통상의 방법으로 추가로 농축 및 여과하여 얻어진 더욱 순도 높은 잣나무잎 추출물을 사용할 수 있다. 수증기 증류 추출법에 의하여, 잣나무잎 추출물은 잣나무 향을 띄는 맑은 무색투명한 기름(오일)의 형태(증류추출오일)로 수득할 수 있다.The steam distillation extraction method is well known in the art, and the ratio of the pine tree leaves and purified water for performing the steam distillation extraction is 1:1 to 1:10, specifically 1:1 to 1:3, more specifically 1 : 2 (by weight). The pine needles and purified water are distilled once or multiple times in a distillation tank at 70 to 500°C, specifically 80 to 150°C, more specifically 90°C, for about 1 hour to several days, specifically 3 to 4 hours. . The condensate obtained by cooling and condensing the distillate thus obtained can be used in the present invention. Further, the condensate thus obtained may be further concentrated and filtered by a conventional method in the art to obtain a higher purity pine tree leaf extract. By the steam distillation extraction method, the pine tree leaf extract can be obtained in the form of a clear, colorless and transparent oil (oil) with a pine tree scent (distilled extraction oil).
상기 잣나무 잎 추출물은 질염의 주요 원인균인 가드네렐라 바지날리스(Gardnerella vaginalis), 칸디다 알비칸스(Candida albicans), 스테필로코커스 아우레우스(Staphylococcus aureus)에 항균활성을 가지므로, 본 발명의 잣나무 잎 추출물은 질 내 유해 세균을 억제 또는 사멸시켜 질염 예방 또는 치료용 조성물로서 사용될 수 있다.The pine tree leaf extract has antibacterial activity against Gardnerella vaginalis, Candida albicans, and Staphylococcus aureus, which are major causative bacteria of vaginitis. Leaf extract can be used as a composition for preventing or treating vaginitis by inhibiting or killing harmful bacteria in the vagina.
본 발명의 일 실시예에서는 잣나무 잎 추출물을 처리하는 경우, 질염의 주요 원인균인 가드네렐라 바지날리스, 칸디다 알비칸스, 스테필로코커스 아우레우스에 대한 항균활성이 우수함을 확인하였다(표 1).In an embodiment of the present invention, it was confirmed that the antibacterial activity was excellent against the main causative bacteria of vaginitis , Gardnerella parangalis , Candida albicans , and Staphylococcus aureus (Table 1). .
본 발명의 다른 측면은 상기 잣나무 잎 추출물을 포함하는 질염 치료 또는 예방용 약학적 조성물을 제공한다.Another aspect of the present invention provides a pharmaceutical composition for the treatment or prevention of vaginitis comprising the pine tree leaf extract.
상기 “예방”은 본 발명에 따른 약학적 조성물의 투여에 의해 질염을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.The “prevention” refers to any action that suppresses vaginitis or delays the onset of vaginitis by administration of the pharmaceutical composition according to the present invention.
상기 “치료”는 본 발명에 따른 약학적 조성물의 투여에 의해 질염에 대한 증세가 호전되거나 이롭게 변경되는 모든 행위를 의미한다.The “treatment” refers to any action in which symptoms of vaginitis are improved or beneficially changed by administration of the pharmaceutical composition according to the present invention.
상기 약학적 조성물은 유효성분 이외에 약학적으로 허용되는 담체를 포함할 수 있다. 이때, 약학적으로 허용되는 담체는 제제 시에 통상적으로 이용되는 것으로서, 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아고무, 인산 칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세 결정성셀룰로스, 폴리비닐피로리돈, 셀룰로스, 물, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필 히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일등을 포함하나, 이에 한정되는 것은 아니다. 또한, 상기성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다.The pharmaceutical composition may include a pharmaceutically acceptable carrier in addition to the active ingredient. At this time, the pharmaceutically acceptable carrier is commonly used in the formulation, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose , Polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil, but are not limited thereto. In addition, a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like may be additionally included in addition to the above components.
상기 약학적 조성물은 목적하는 방법에 따라 경구 투여하거나 비경구투여(예를 들어, 정맥 내, 피하, 복강 내 또는 국소에 적용)할 수 있으며, 투여량은 환자의 상태 및 체중, 질병의 정도, 약물형태, 투여경로 및 시간에 따라 다르지만, 당업자에 의해 적절하게 선택될 수 있다.The pharmaceutical composition may be administered orally or parenterally according to a desired method (for example, intravenously, subcutaneously, intraperitoneally or topically), and the dosage may be the condition and weight of the patient, the degree of disease, It depends on the drug form, administration route and time, but may be appropriately selected by those skilled in the art.
상기 약학적 조성물은 약학적으로 유효한 양으로 투여한다. 상기 “약학적으로 유효한 양”은 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분한 양을 의미하며, 유효용량 수준은 환자의 질환의 종류, 중증도, 약물의 활성, 약물에 대한 민감도, 투여 시간, 투여 경로 및 배출비율, 치료기간, 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다.The pharmaceutical composition is administered in a pharmaceutically effective amount. The “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, and drug Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field.
상기 약학적 조성물은 개별 치료제로 투여하거나 다른 치료제와 병용하여 투여될 수 있고 종래의 치료제와는 순차적 또는 동시에 투여될 수 있으며, 단일 또는 다중 투여될 수 있다. 상기한 요소들을 모두 고려하여 부작용 없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하며, 이는 당업자에 의해 용이하게 결정될 수 있다.The pharmaceutical composition may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, which can be easily determined by a person skilled in the art.
본 발명의 또 다른 측면은 상기 약학적 조성물을 개체에 투여하는 단계를 포함하는 질염 치료방법을 제공한다.Another aspect of the present invention provides a method for treating vaginitis comprising administering the pharmaceutical composition to a subject.
상기 "개체"란 질병의 치료를 필요로 하는 대상을 의미하고, 보다 구체적으로는, 인간 또는 비-인간인 영장류, 생쥐(mouse), 개, 고양이, 말 및 소 등의 포유류를 의미한다.The "individual" refers to a subject in need of treatment of a disease, and more specifically, refers to a mammal such as a human or non-human primate, mouse, dog, cat, horse and cow.
구체적으로 상기 약학적 조성물의 유효량은 환자의 연령, 성별, 상태, 체중, 체내에 활성 성분의 흡수도, 불활성율 및 배설속도, 질병종류, 병용되는 약물에 따라 달라질 수 있으며, 일반적으로는 체중 1kg 당 0.001 내지 150mg, 바람직하게는 0.01내지 100mg을 매일 또는 격일 투여하거나, 1일 1 내지 3회로 나누어 투여할 수 있다. 그러나 투여 경로, 비만의 중증도, 성별, 체중, 연령 등에 따라서 증감 될 수 있으므로 상기 투여량이 어떠한 방법으로도 본 발명의 범위를 한정하는 것은 아니다.Specifically, the effective amount of the pharmaceutical composition may vary depending on the patient's age, sex, condition, body weight, absorption of the active ingredient in the body, the inactivation rate and excretion rate, the type of disease, and the drug to be used in combination. 0.001 to 150 mg per sugar, preferably 0.01 to 100 mg, may be administered daily or every other day, or divided into 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, the severity of obesity, sex, weight, age, etc., the dosage amount does not limit the scope of the present invention in any way.
본 발명의 또 다른 측면은 잣나무 잎 추출물을 포함하는 질염 예방 또는 개선용 식품 조성물을 제공한다.Another aspect of the present invention provides a food composition for preventing or improving vaginitis comprising a pine tree leaf extract.
상기 “개선”은 치료되는 상태와 관련된 파라미터, 예를 들면 증상의 정도를 적어도 감소시키는 모든 행위를 의미한다.The "improvement" means any action that at least reduces the severity of a parameter related to the condition being treated, for example a symptom.
상기 식품은 육류, 스낵류, 낙농제품, 음료수 등을 예시할 수 있으나 이에 한정되는 것은 아니며, 통상적인 기능성 식품을 모두 포함하는 개념으로 이해될 수 있다.The food may exemplify meat, snacks, dairy products, beverages, and the like, but is not limited thereto, and may be understood as a concept including all conventional functional foods.
상기 식품 조성물은 통상의 식품 첨가물을 포함할 수 있으며, 상기 식품 첨가물은 다른 규정이 없는 한 식품의약품안전처에 승인된 식품 첨가물 공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의하여 적합성 여부를 판단할 수 있다.The food composition may contain ordinary food additives, and the food additives are subject to the standards and standards for the relevant item according to the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. Whether it is appropriate or not can be judged.
상기 식품 첨가물 공전에 기재된 품목은 예컨대 케톤류, 글리신, 구연산칼륨, 니코틴산, 계피산 등의 화학적 합성물, 감색소, 감초추출물, 결정셀룰로오스, 고량색소, 구아검 등의 천연첨가물, L-글루타민산나트륨 제제, 면류첨가알칼리제, 보존료제제, 타르색소제제 등의 혼합제제류를 들 수 있다.The items described in the above food additive code include, for example, ketones, glycine, potassium citrate, nicotinic acid, cinnamic acid, and other chemical compounds, red pigment, licorice extract, crystalline cellulose, high color pigments, natural additives such as guar gum, sodium L-glutamate preparations, and noodles. Mixed preparations, such as an added alkali agent, a preservative preparation, and a tar color preparation, are mentioned.
상기 식품 조성물은 질염의 개선을 위한 식품 및 음료 등에 다양하게 이용될 수 있으며, 예컨대, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 건강기능성 보조 식품, 식품 첨가제 등에 사용될 수 있다.The food composition may be variously used in foods and beverages for improving vaginitis, and for example, various foods, beverages, gum, tea, vitamin complexes, health functional supplements, food additives, and the like.
상기 식품 조성물은 질염의 개선을 목적으로, 전체 중량 대비 0.001 내지 80.0 중량%의 상기 조성물을 포함할 수 있다. 상기 조성물이 0.001 중량% 미만이면 질염의 개선 효과가 충분히 구현되지 않을 수 있고 80.0 중량% 초과이면 제품 본연의 품질이 구현되지 않거나 비용 효율이 저하될 수 있다.The food composition may include 0.001 to 80.0% by weight of the composition based on the total weight for the purpose of improving vaginitis. If the composition is less than 0.001% by weight, the effect of improving vaginitis may not be sufficiently realized, and if it exceeds 80.0% by weight, the original product quality may not be realized or cost efficiency may be lowered.
상기 식품 조성물은 질염의 개선을 목적으로 정제, 과립, 분말, 캅셀, 액상의 용액 및 환으로 이루어진 군에서 선택된 어느 하나의 제형으로 제조 및 가공될 수 있다.The food composition may be prepared and processed into any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills for the purpose of improving vaginitis.
구체적으로 상기 정제 형태의 기능성 식품은 잣나무 잎 추출물 또는 이의 분획물, 부형제, 결합제, 붕해제 및 다른 첨가제와의 혼합물을 통상의 방법으로 과립화한 다음, 활택제 등을 넣어 압축 성형하거나, 상기 혼합물을 직접 압축 성형하여 제조할 수 있다. 또한, 상기 정제 형태의 기능성 식품은 필요에 따라 교미제 등을 함유할 수 있으며, 필요에 따라 적당한 제피제로 제피할 수도 있다.Specifically, the functional food in the form of tablets is a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, and other additives are granulated by a conventional method, and then compression molded by putting a lubricant or the like, or the mixture is It can be produced by direct compression molding. In addition, the functional food in the form of a tablet may contain a mating agent or the like as necessary, and may be coated with a suitable coating agent if necessary.
상기 캅셀 형태의 식품 조성물 중 경질캅셀제는 통상의 경질캅셀에 가지 추출물 및 부형제 등의 첨가제와의 혼합물 또는 그의 입상물 또는 제피한 입상물을 충진하여 제조할 수 있으며, 연질캅셀제는 목본류 추출물 또는 이의 분획물, 및 부형제 등의 첨가제와의 혼합물을 젤라틴 등 캅셀기제에 충진하여 제조할 수 있다. 상기 연질캅셀제는 필요에 따라 글리세린 또는 솔비톨 등의 가소제, 착색제, 보존제 등을 함유할 수 있다.Among the capsule-type food compositions, hard capsules can be prepared by filling a conventional hard capsule with a mixture of additives such as eggplant extracts and excipients, or granules or skinned granules thereof, and soft capsules are wood extracts or fractions thereof. , And a mixture of additives such as excipients can be prepared by filling a capsule base such as gelatin. The soft capsules may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, and the like, if necessary.
상기 환 형태의 식품 조성물은 잣나무 잎 추출물 또는 이의 분획물, 부형제, 결합제, 붕해제 등의 혼합물을 적당한 방법으로 성형하여 조제할 수 있으며, 필요에 따라 백당이나 다른 적당한 제피제로 제피를, 또는 전분, 탈크 또는 적당한 물질로 환의를 입힐 수도 있다.The ring-shaped food composition may be prepared by molding a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, etc. in an appropriate way, and if necessary, a coating agent, or starch, talc Alternatively, you can make a ring with a suitable substance.
상기 과립형태의 식품 조성물은 잣나무 잎 추출물 또는 이의 분획물, 부형제, 결합제, 붕해제 등의 혼합물을 적당한 방법으로 입상으로 제조할 수 있으며, 필요에 따라 착향제, 교미제 등을 함유할 수 있다The granular food composition may be prepared in a granular form of a mixture of pine needles leaf extract or its fractions, excipients, binders, disintegrants, etc., and may contain flavoring agents, flavoring agents, etc., if necessary.
또한, 상기 부형제, 결합제, 붕해제, 활택제, 교미제, 착향제 등에 대한 용어 정의는 당업계에 공지된 문헌에 기재된 것으로 그 기능 등이 동일 내지 유사한 것들을 포함할 수 있다.In addition, the definitions of terms for the excipients, binders, disintegrants, lubricants, flavoring agents, flavoring agents, and the like are described in documents known in the art, and may include those having the same or similar functions.
본 발명의 또 다른 측면은 잣나무 잎 추출물을 포함하는 질염 개선 또는 예방용 화장료 조성물을 제공한다.Another aspect of the present invention provides a cosmetic composition for improving or preventing vaginitis comprising a pine needle extract.
상기 화장료 조성물은 유연화장수, 영양화장수, 수분크림, 영양크림, 마사지크림, 영양로션, 에센스, 앰플, 젤, 아이크림, 오일, 파운데이션, 클렌징크림, 클렌징폼, 클렌징워터, 샴푸, 린스, 팩, 스프레이 및 파우더로 이루어진 군에서 선택된 하나 이상으로 제형화될 수 있다.The cosmetic composition includes softening lotion, nutritional lotion, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, shampoo, conditioner, pack, It may be formulated as one or more selected from the group consisting of spray and powder.
상기 화장료 조성물은 통상의 방법에 의해 제형화될 수 있다. 피부 외용제의 제형화에 있어서 Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA에 개시되어 있는 내용을 참조할 수 있고, 화장료 조성물의 제형화에 있어서 International cosmetic ingredient dictionary, 6th ed(The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995)에 개시되어 있는 내용을 참조할 수 있다.The cosmetic composition may be formulated by a conventional method. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the content disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions. , Inc., Washington, 1995).
구체적으로, 상기 화장료 조성물은 일반적인 유화 제형 및 가용화 제형으로 제조할 수 있다. 예컨대, 유연 화장수 또는 영양 화장수 등의 화장수; 훼이셜 로션, 바디로션 등의 유액; 영양 크림, 수분 크림, 아이 크림 등의 크림; 에센스; 화장연고; 스프레이; 젤; 팩; 선 스크린; 메이크업 베이스; 액체 타입, 고체 타입 또는 스프레이 타입 등의 파운데이션; 파우더; 클렌징 크림, 클렌징 로션, 클렌징 오일 등의 메이크업 제거제; 또는 클렌징 폼, 비누, 바디워시 등의 세정제로 제형화될 수 있으나 이에 한정되는 것은 아니다. 또한 상기 피부 외용제는, 연고, 패치, 겔, 크림 또는 분무제로 제형화될 수 있으나 이에 한정되는 것은 아니다.Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial lotion and body lotion; Creams such as nourishing cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Alternatively, it may be formulated with a detergent such as cleansing foam, soap, and body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.
상기 화장료 조성물은 각각의 제형에 있어서 상기 필수성분 외에 제형의 종류 또는 사용 목적 등에 따라 본 발명에 따른 목적을 저해하지 않는 범위 내에서 다른 성분들이 적절히 배합될 수 있다.In the cosmetic composition, in addition to the essential ingredients in each formulation, other ingredients may be appropriately blended within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use.
상기 화장료 조성물은 통상적으로 허용 가능한 담체를 포함할 수 있으며, 예컨대 유분, 물, 계면활성제, 보습제, 저급 알코올, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 한정되는 것은 아니다.The cosmetic composition may contain a generally acceptable carrier, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.
상기 허용 가능한 담체는 제형에 따라 달리할 수 있다. 예컨대, 연고, 페이스트, 크림 또는 젤로 제형화될 때 담체 성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 또는 이들의 혼합물이 사용될 수 있다The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or their Mixtures can be used
상기 화장료 조성물은 파우더 또는 스프레이로 제형화될 때, 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록사이드, 칼슘 실케이트, 폴리아미드 파우더 또는 이들의 혼합물이 사용될 수 있고, 스프레이의 경우 클로로플루오로히드로카본, 프로판, 부탄 또는 디메틸 에테르와 같은 추진제를 더 포함할 수 있다.When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.
상기 화장료 조성물은 용액 또는 유탁액으로 제형화될 때, 담체 성분으로서 용매, 용해화제, 또는 유탁화제가 사용될 수 있고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-브틸글리콜 오일이 사용될 수 있고, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브 오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 사용될 수 있다. When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.
상기 화장료 조성물은 현탁액으로 제형화될 때, 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리 옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 사용될 수 있다.When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.
상기 화장료 조성물이 비누로 제형화될 때, 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알코올, 식물성 유, 글리세롤, 당 등이 사용될 수 있다.When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. Can be used.
상기 화장료 조성물은 최종 제품의 품질이나 기능에 따라 업계에서 통상적으로 사용되는 지방 물질, 유기용매, 용해제, 농축제, 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉 쇄제, 킬레이트화제, 보존제, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품학 또는 피부과학 분야에서 통상적으로 사용되는 보조제를 추가적으로 함유할 수 있다.The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.
다만, 상기 보조제 및 그 혼합 비율은 본 발명에 따른 화장료 조성물의 바람직한 성질에 영향을 미치지 않도록 적절히 선택할 수 있다.However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.
본 발명은 질염 원인균인 가드네렐라 바지날리스(Gardnerella vaginalis), 칸디다 알비칸스(Candida albicans) 및 스테필로코커스 아우레우스(Staphylococcus aureus)에 대한 항균활성을 갖는 잣나무 잎 추출물 및 이를 포함하는 질염 치료 및 예방용 조성물에 관한 것으로, 천연물인 잣나무 잎 추출물은 인체에 대한 독성이 낮고, 상기 원인균에 대한 항균 효과가 우수하므로 질염의 예방, 개선 내지 치료에 유용하게 활용될 수 있다.The present invention is a vaginitis causative bacteria Gardnerella vaginalis (Gardnerella vaginalis), Candida albicans (Candida albicans) and It relates to a pine tree leaf extract having antibacterial activity against Staphylococcus aureus, and a composition for treating and preventing vaginitis comprising the same, wherein the natural product, pine leaf extract, has low toxicity to the human body, and against the causative bacteria. Since the antibacterial effect is excellent, it can be usefully used in the prevention, improvement or treatment of vaginitis.
본 발명의 효과는 상기한 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 청구범위에 기재된 발명의 구성으로부터 추론 가능한 모든 효과를 포함하는 것으로 이해되어야 한다.The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명이 하기 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.
실시예 1. 잣나무 잎 추출물의 제조Example 1. Preparation of pine leaf extract
신선한 잣나무 잎(경기도 가평군, 강원 홍천군)을 수집하고 병들거나 벌레먹은 것, 마른 것을 선별해서 제거하고, 선별한 잣나무 잎을 세척탱크에 넣고 흐르는 물로 3번 세척하였다.Fresh pine pine leaves (Gapyeong-gun, Gyeonggi-do, Hongcheon-gun, Gangwon-do) were collected, diseased, insect-eaten, and dry ones were selected and removed, and the selected pine needles were placed in a washing tank and washed three times with running water.
세척한 잣나무 잎을 3일간 음건(陰乾)하고, 이를 절삭기를 이용하여 10mm가량으로 일정하게 절단하였다. 절단된 잣나무을 증류탱크로 옮겼다. The washed pine needles were dried in the shade for 3 days, and they were regularly cut into about 10 mm using a cutting machine. The cut pine trees were transferred to a distillation tank.
증류탱크에 잣나무 잎과 정제수의 비율을 1 : 2 정도로 하여 넣은 후 증류탱크의 잣나무 잎의 추출온도를 90 ℃를 유지시켜 3 내지 4 시간 증류시켰다. 이때 냉각기 내부에 공급되는 냉각수의 온도는 18 ℃정도로 유지하며, 냉각기를 통과한 응축물을 응축물 분리기에 수집하였다.After putting the pine tree leaves and purified water in the distillation tank at a ratio of 1:1, the extraction temperature of the pine trees leaves of the distillation tank was maintained at 90°C and distilled for 3 to 4 hours. At this time, the temperature of the cooling water supplied to the inside of the cooler was maintained at about 18°C, and the condensate passed through the cooler was collected in a condensate separator.
수집된 응축물은 기름과 물이 혼재되어 있기 때문에 추가의 농축 과정과 여과 과정을 거처 순도 높은 추출물을 얻었다. 얻어진 추출물은 맑은 무색 투명한 기름으로 잣나무향을 나타내었다. 상기 추출과정으로 얻어진 최종 추출물을 냉암소에서 최종 화학성분의 밸런스(안정화)와 풍미와 향을 위해 30 일 정도 숙성시켰다Since the collected condensate was mixed with oil and water, it was further concentrated and filtered to obtain a high-purity extract. The obtained extract was a clear, colorless and transparent oil that exhibited a pine scent. The final extract obtained by the above extraction process was aged for about 30 days for the balance (stabilization) and flavor and aroma of the final chemical components in a cool and dark place.
실시예 2. 질염 원인균에 대한 항균 활성 확인Example 2. Confirmation of antibacterial activity against vaginitis causative bacteria
잣나무 잎에서 추출한 잣나무 잎 추출물의 항균 활성을 디스크 확산법을 이용하여 확인하였다. 대상 균주로 질염 원인균인 가드네렐라 바지날리스(Gardnerella vaginalis), 칸디다 알비칸스(Candida albicans), 스테필로코커스 아우레우스(Staphylococcus aureus)를 사용하였으며, 각각 최적배지에서 2 회 이상 계대 배양하여 활성화시켜 준비하였다.The antimicrobial activity of the pine needle leaf extract extracted from the pine needle leaf was confirmed using the disk diffusion method. Gardnerella vaginalis, Candida albicans, and Staphylococcus aureus, which are the cause of vaginitis, were used as target strains, and activated by subculture at least two times in each optimal medium. Prepared.
균 현탁액은 멸균수로 스테필로코커스 아우레우스는 탁도 0.5(약 108 CFU/mL), 칸디다 알비칸스는 탁도 1.0이 되도록 희석하여 50 μL를 취한 후 5 mL의 top agar(0.75 % agar 첨가)에 접종하여 디스크 확산법 측정용 평판배지(Sabouraud Dextrose Agar) 위에 분주하였다. 그 위에 멸균된 paper disc(8mm, Whatman)를 올려놓은 다음 잣나무 잎 추출물 20 μL를 분주하였다. Dilute the bacterial suspension with sterile water to have a turbidity of 0.5 (about 10 8 CFU/mL) for Stephylococcus aureus and 1.0 for Candida albicans, take 50 μL, and add 5 mL of top agar (add 0.75% agar). Was inoculated and dispensed on a plate medium (Sabouraud Dextrose Agar) for measuring the disk diffusion method. A sterilized paper disc (8mm, Whatman) was placed on it, and then 20 μL of pine needle leaf extract was dispensed.
이때, 가드네렐라 바지날리스의 경우는 혈액배지(Blood Agar Plate)를 이용하였기 때문에 도말평판배양(spread plate culture)법으로 실험하였다. 균 현탁액의 탁도를 1.0으로 조정한 다음 평판배지에 100 μL를 분주하여 도말봉으로 도말한 다음 잣나무 잎 추출물 20 uL를 분주하였다. At this time, in the case of Gardnerella vaginalis, since Blood Agar Plate was used, the experiment was conducted by spread plate culture. The turbidity of the bacterial suspension was adjusted to 1.0, and then 100 μL was dispensed on a plate medium, smeared with a smear, and 20 uL of the white pine leaf extract was dispensed.
각 균주별 현탁액에 잣나무 잎 추출물을 분주한 샘플을 배양기에서 배양하고, 저해환의 지름(mm)을 측정하여 항균력을 측정하여 표 1에 나타내었다.Samples dispensed with pine needles leaf extract into the suspension for each strain were cultured in an incubator, and the diameter (mm) of the inhibitory ring was measured to measure the antibacterial activity and are shown in Table 1.
(Gardnerella vaginalis)(Gardnerella vaginalis)
(Candida albicans)(Candida albicans)
(Staphylococcus aureus)(Staphylococcus aureus)
상기 표 1에 나타난 바와 같이, 잣나무 잎 추출물이 잣송이 또는 솔잎과 같은 유사종 추출물들에 비해 억제 범위가 크다는 사실을 확인하였다. 또한, 칸디다 알비칸스와 같은 곰팡이 균에 대해서는 잣나무 잎 추출물만 억제 효과를 가지는 것을 확인할 수 있었으며, 본 발명의 잣나무 잎 추출물이 질염의 원인균 3 종 모두에 대해 동시에 항균 활성을 가진다는 것을 확인할 수 있었다.나아가, 질염의 원인균 3종 모두에 대해 항균 활성을 나타내는 바, 질염의 원인균 억제를 통해 질염에 대한 치료 및 예방 용도로 사용될 수 있음을 확인하였다.As shown in Table 1, it was confirmed that the pine needle leaf extract had a larger inhibitory range than the extracts of similar species such as pine pine or pine needles. In addition, for fungi such as Candida albicans, it was confirmed that only the pine tree leaf extract has an inhibitory effect, and it was confirmed that the pine tree leaf extract of the present invention has antibacterial activity against all three causative bacteria of vaginitis at the same time. Furthermore, it was confirmed that it can be used for the treatment and prevention of vaginitis by inhibiting the causative agent of vaginitis as it exhibits antibacterial activity against all three causative bacteria of vaginitis.
전술한 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 예를 들어, 단일형으로 설명되어 있는 각 구성 요소는 분산되어 실시될 수도 있으며, 마찬가지로 분산된 것으로 설명되어 있는 구성 요소들도 결합된 형태로 실시될 수 있다.The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.
본 발명의 범위는 후술하는 청구범위에 의하여 나타내어지며, 청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be interpreted as being included in the scope of the present invention.
Claims (7)
가드네렐라 바지날리스(Gardnerella vaginalis), 칸디다 알비칸스(Candida albicans) 및 스테필로코커스 아우레우스(Staphylococcus aureus) 로 이루어진 군에서 선택된 하나 이상에 대한 항균 조성물.Containing Pinus koraiensis SIEB leaf extract,
Gardnerella vaginalis, Candida albicans and Antimicrobial composition for one or more selected from the group consisting of Staphylococcus aureus .
상기 추출물은 물, 알코올, 에틸아세테이트, 아세톤, 핵산, 디클로로메탄 또는 이들의 혼합 용매로 추출된 것인 항균 조성물.The method of claim 1,
The extract is water, alcohol, ethyl acetate, acetone, nucleic acid, dichloromethane, or an antimicrobial composition extracted with a mixed solvent thereof.
상기 잣나무 잎 추출물은 수증기 증류 추출법에 의하여 수득된 것인 항균 조성물.The method of claim 1,
The pine tree leaf extract is an antibacterial composition obtained by a steam distillation extraction method.
상기 잣나무 잎 추출물은 오일 형태인 것인, 항균 조성물.The method of claim 1,
The pine tree leaf extract is in the form of an oil, antibacterial composition.
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