KR102289506B1 - Anti-bacterial composition for Streptococcus mutans and composition for removing halitosis using the same - Google Patents

Abstract

The present invention discloses an antibacterial composition for Streptococcus mutans, a causative bacterium of dental caries, and a composition for removing bad breath using the same, using an arborvitae leaf extract, a loquat leaf extract, and the like.

Description

Antibacterial composition for Streptococcus mutans and a composition for removing bad breath using the same

The present invention relates to an antibacterial composition for Streptococcus mutans and a composition for removing bad breath using the same.

The importance of oral health management to improve the quality of life of the elderly is increasing as the average life expectancy is extended due to the improvement of people's living standards and the development of medical technology. Dental caries and periodontal disease are typical diseases that can cause tooth loss due to oral health problems. According to data from the Ministry of Health and Welfare in 2013, the experience rate of permanent dental caries among Korean adult males was 87%, and that of females was 90%. In addition, the prevalence of periodontal disease among Korean adults aged 30 years and older decreased from 2007 to 2012, and then increased again in 2013.

Dental caries is experienced by more than 80% of the world's population, and although the incidence has continuously decreased since the 1970s in some developed countries in the West, it is still increasing in Eastern Europe and some African regions, including Korea (Nishi et al., 2002 Community Dent) (Nishi et al., 2002 Community Dent). Oral Epidemiol 2002 30:296-301.). Dental caries not only causes various pains, but also causes apical, alveolar bone destruction and tooth loss if it continues to progress. The occurrence of dental caries is closely related to dietary habits and oral microorganisms, and the causative bacterium representing dental caries among about 750 types of microorganisms that inhabit the human oral cavity is known as Streptococcus mutans (Loesche WJ. Medical). Microbiology 1996 (4th ed.);Kilian Clarke J. Br J Exp Pathol. 1924 Jun; 5(3): 141147.; Hyeyoung Kim, Seoul National University doctoral thesis 2002).

When Streptococcus mutans comes in contact with sugar, etc. in the oral cavity, polysaccharide crystals are created and stored between the teeth. It attaches to the tooth film and synthesizes insoluble glucan by the action of glucosyltransferase (GTase) enzyme. The synthesized glucan increases the binding force between bacteria and lactic acid formed as metabolic waste accumulates under the polysaccharide crystals and is concentrated. It is known that this concentrated high concentration of lactic acid ^{dissolves Ca 2+} in calcium phosphate, which is a component of teeth, and causes dental caries by making holes in the tooth surface enamel (Sigmund, SS et al., 1992 J. Periodontol. 63 :322-331.; Kim et al., Hyunmoonsa 2009 19:307-316). Accordingly, in order to prevent dental caries, the search for an effective antibacterial agent for Streptococcus mutans or a substance having an inhibitory activity of GTase is being actively conducted.

In the case of continuous use of general antibacterial agents to treat dental caries, a chronic disease, various side effects such as digestive disorders, hypersensitivity reactions, kidney toxicity, oral toxicity, and tooth discoloration may occur. The search for materials is continuously being attempted. Accordingly, Korean Patent No. 1015449390000 discloses a composition for preventing or treating dental caries comprising Jingyo extract as an active ingredient, Korean Patent No. 1015069950000, Orinaru bark extract, Korean Patent No. 1013158050000, Japanese cypress extract, Korean Patent Registration No. 1012952420000 discloses a composition for preventing or treating dental caries, which includes a gourd extract as an active ingredient. .

The present invention discloses antibacterial activity, such as arborvitae leaf extract, against Streptococcus mutans, a causative agent of dental caries.

It is an object of the present invention to provide an antibacterial composition against Streptococcus mutans using an extract of arborvitae leaf and the like.

Another object of the present invention is to provide a composition for removing bad breath using an extract of arborvitae leaf and the like.

Other objects or specific objects of the present invention will be set forth below.

As confirmed in the Examples and Experimental Examples below, the present invention is, by the disc-agar plate diffusion method, a arborvitae leaf extract, a loquat leaf extract, and a mixture thereof, which is a causative agent of dental caries, streptococcal. Coccus mutans ( S. mutans ) with antibacterial activity and other harmful microorganisms E. coli ( E. coli ), Salmonella enterica ( S. enterica ), Vibrio para haemolyticus ( V. parahaemolyticus ), Bacillus cereus ( B. cereus ) It has antibacterial activity, and also has an antibacterial effect for oral hygiene, an effect of removing bad breath, etc., and is completed by confirming that the irritation is low and the preference such as flavor is excellent.

Considering the above, in one aspect, the present invention provides an antibacterial composition for Streptococcus mutans or a composition for improving dental caries comprising an arborvitae leaf extract, a loquat leaf extract, or a mixture thereof as an active ingredient. In another aspect, it can be identified as a bad breath remover composition comprising an arborvitae leaf extract, a loquat leaf extract or a mixture thereof as an active ingredient, and in another aspect, an arborvitae leaf extract, a loquat leaf extract or It can be identified as an antibacterial composition for Streptococcus mutans, Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus, and Bacillus cereus containing a mixture thereof as an active ingredient.

As used herein, the term "extract" refers to water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, Extracts obtained by leaching using N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, carbon dioxide, pentane, etc. It means an extract obtained, a fraction obtained by fractionating the extract, or an essential oil extract, and the extraction method is an arbitrary method such as chilling, reflux, heating, ultrasonic radiation, supercritical extraction, etc. can be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the crude extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it refers to an essential oil extract or obtained by extraction with water, ethanol, or a mixed solvent thereof as an extraction solvent, particularly 60% to 90% of an aqueous ethanol solution. In general, an essential oil extract refers to an oily extract obtained by condensing a volatile component. For the preparation of the essential oil extract, methods known in the art, such as steam distillation, solvent extraction using a volatile solvent (benzene, hexane, etc.), and supercritical extraction may be used.

In addition, as used herein, the term "active ingredient" refers to a component capable of exhibiting the desired activity alone or in combination with a carrier that has no activity by itself.

In addition, in the present specification, "improving dental caries" means preventing, treating, or alleviating symptoms of dental caries, which is a target disease.

Also, in the present specification, "antibacterial" is meant to include induction of death or inhibition of proliferation of the corresponding microorganism.

The antibacterial composition of the present invention or the composition for improving periodontal caries (hereinafter collectively referred to as the "composition of the present invention") may exhibit the intended antibacterial activity, dental caries improvement activity, etc. It may be included in any amount (effective amount) as long as the effective amount will be determined within the range of 0.001% to 15% by weight based on the total weight of the composition. Herein, the term "effective amount" refers to the intended medical and pharmacological effects such as improvement of dental caries, etc., when the composition of the present invention is administered to a mammal, preferably a human, to which it is applied during the administration period as suggested by a medical professional. It refers to the amount of active ingredient that can be displayed. Such effective amounts can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In a specific aspect, the composition of this invention can be grasped|ascertained as a food composition.

The food composition of the present invention may be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, ionic beverages, processed oils such as milk and yogurt, gums, rice cakes, Korean sweets, bread, confectionery, noodles, etc. of foods, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, bars and the like health functional food preparations. In addition, the food composition of the present invention may have any product classification in terms of legal and functional classification as long as it conforms to the enforcement laws at the time of manufacture and distribution. For example, it is a health functional food according to the Health Functional Food Act, or confectionery, beans, soy milk, fermented beverages, special purpose food, etc. can

The food composition of the present invention may contain food additives in addition to the active ingredients thereof. Food additives are generally understood as substances that are added to, mixed or infiltrated with food in manufacturing, processing, or preserving food. The Food Additives Ordinance (Ministry of Food and Drug Safety Notice, Food Additive Standards and Specifications) according to the Food Sanitation Act restricts food additives with guaranteed safety by classifying them into chemically synthesized products, natural additives, and mixed preparations.

These food additives may be classified into sweeteners, flavoring agents, preservatives, emulsifiers, acidulants, thickeners, and the like in terms of functionality.

The sweetener is used to impart appropriate sweetness to food, and natural or synthetic ones may be used. Preferably, a natural sweetener is used. Examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents may be used to improve taste or aroma, and both natural and synthetic ones may be used. Preferably, it is a case where a natural thing is used. In the case of using a natural one, the purpose of nutritional enhancement in addition to flavor may be concurrently used. As a natural flavoring agent, it may be obtained from apple, lemon, tangerine, grape, strawberry, peach, etc., or it may be obtained from green tea leaf, dandelion, bamboo leaf, cinnamon, chrysanthemum leaf, jasmine, etc. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo biloba, etc. can be used. The natural flavoring agent may be a liquid concentrate or a solid extract. Optionally, a synthetic flavoring agent may be used, and the synthetic flavoring agent may be an ester, an alcohol, an aldehyde, a terpene, or the like.

As a preservative, sodium calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, Pectin etc. are mentioned, and acidulant, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, etc. can be used as an acidulant. Acidulant may be added so that the food composition has an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste.

As the thickening agent, a suspending agent, a settling agent, a gel-forming agent, a bulking agent, and the like can be used.

The food composition of the present invention may contain, in addition to the food additives as described above, physiologically active substances or minerals known in the art for the purpose of supplementing and reinforcing functionality and nutrition and guaranteed stability as food additives.

Examples of such physiologically active substances include catechins contained in green tea and the like, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, and the like. Magnesium preparations, such as iron preparations, such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, etc. are mentioned.

In the food composition of the present invention, the food additives as described above may be included in an appropriate amount to achieve the purpose of the addition according to the type of product.

In relation to other food additives that may be included in the food composition of the present invention, reference may be made to the Food Codex or the Food Additives Codex.

The composition of the present invention may be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier in addition to the active ingredient by a conventional method known in the art, such as a mouthwash composition formulation or a quasi-drug formulation such as a toothpaste formulation, an oral formulation, or a parenteral formulation. It can be prepared in pharmaceutical formulations. Here, "pharmaceutically acceptable" means that it does not inhibit the activity of an active ingredient and does not have toxicity beyond what the application (prescription) target can adapt.

When the composition of the present invention is prepared as a mouthwash composition formulation or a toothpaste composition formulation, it may be prepared in a paste form, powder form, spray form or liquid (gargle form), and in addition to containing carnosic acid as the active ingredient of the present invention, It may further include any one or more components selected from the group consisting of an anti-cavity drug component, an abrasive component, a wetting agent component, a binder component, a foaming component, a sweetener component, a fragrance component, and a color component.

Any one or more ingredients selected from the group consisting of sodium fluoride, sodium fluorophosphate, amine fluoride, tin fluoride, chlorhexidine, cetylpyridium chloride, triclosan, xylitol, bamboo salt, and propolis can be used as the tooth decay prevention medicinal ingredient there is.

In addition, the abrasive component is selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate, and aluminum silicate. Any one or more components may be used.

In addition, as the humectant component, any one or more selected from the group consisting of glycerin, sorbitol, xylitol, polyethylene glycol and propylene glycol may be used. This humectant component is an essential base component for making a paste formulation. When the toothpaste is exposed to the air, it prevents drying and solidification, and provides gloss to the surface of the toothpaste. Depending on the type, it plays a role in giving a sweetening effect when brushing teeth. .

In addition, as the binder component, any water-soluble polymer may be used. Preferably, sodium carboxymethyl cellulose, carrageenans, xanthan gum, etc. may be used. The binder acts to prevent the solid powder component from being separated from the liquid component.

In addition, as the foaming agent component, it is preferable to use sodium lauryl sulfate, an anionic surfactant, and depending on the characteristics of the formulation, a copolymer of polyoxyethylene polyoxypropylene (poloxamer), polyoxyethylene hydrogenated castor oil or polyoxy Nonionic surfactants, such as ethylene sorbitan fatty acid ester, can be used. These foaming ingredients enhance the feeling of use of the product, help the cleaning action, speed up the dispersion and penetration of other active ingredients, and reduce interfacial tension, thereby allowing foreign substances in the oral cavity to fall off easily.

In addition, the perfume component, sweetener component, color component, etc. are for improving the feeling of use of the toothpaste composition, and it is preferable to use an edible component as the flavor component. As such components, for example, menthol, peppermint oil, spearmint oil, sage, eucal lyptol, methyl salicylate, or fruit extract.

In addition, it is preferable to use sodium saccharin as the sweetener component, and it is preferable to use food coloring as the pigment component.

When the pharmaceutical composition of the present invention is prepared as an oral dosage form, powders, granules, tablets, pills, dragees, capsules, liquids, gels, syrups, suspensions, wafers, together with a suitable carrier according to methods known in the art. It can be prepared in a formulation such as In this case, examples of suitable pharmaceutically acceptable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, Cellulose such as sodium carboxymethylcellulose and hydroxypropylmethylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable and oil. In the case of formulation activity, the formulation may be formulated by including diluents and/or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants as necessary.

When the pharmaceutical composition of the present invention is prepared for parenteral use, it may be formulated in the form of injections, transdermal administrations, nasal inhalants and suppositories together with suitable carriers according to methods known in the art. In the case of formulation for injection, suitable carriers include sterile water, ethanol, polyols such as glycerol or propylene glycol, or mixtures thereof, preferably Ringer's solution, PBS (phosphate buffered saline) containing triethanolamine, or sterile water for injection. , an isotonic solution such as 5% dextrose may be used. When formulated for transdermal administration, it may be formulated in the form of an ointment, a cream, a lotion, a gel, an external solution, a pasta agent, a liniment agent, an air roll, and the like. In the case of nasal inhalants, it can be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. (tween) 61, polyethylene glycols, cacao fat, laurin fat, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, sorbitan fatty acid esters, and the like may be used.

Formulation of pharmaceutical compositions is known in the art, and for more specific details, reference may be made to the literature [Remington's Pharmaceutical Sciences (19th ed., 1995)] and the like. This document is considered a part of this specification.

A preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, sex, age, patient's severity, and administration route. It can be in the range /kg. Administration may be performed once or divided into several times a day. Such dosages are not to be construed as limiting the scope of the invention in any respect.

As described above, according to the present invention, it is possible to provide an antibacterial composition against Streptococcus mutans using arborvitae leaves and the like. The composition of the present invention may be commercialized as a mouthwash, quasi-drugs such as toothpaste, functional food, medicine, and the like.

1 to 5 are results of antibacterial activity.
6 is a result of measuring the number of bacteria.
7 is an analysis result of bad breath gas in the oral cavity.

Hereinafter, the present invention will be described with reference to Examples. However, the scope of the present invention is not limited to these examples.

<Example> Antibacterial activity test for oral microorganisms, etc., oral bacterial count test, oral bad breath gas analysis test and sensory evaluation test

1. Samples and Methods

1.1 Samples

For the ethanol extract sample , 10 times the weight of 80% ethanol was added to each dried powder of Thuja orientalis ( Thuja orientalis , extraction part: leaf), loquat tree ( Eriobotrya Japonica , extraction part: leaf), and extracted at 80 ° C. for 4 hours, followed by filter paper After filtration with (Whatman #2), it was concentrated under reduced pressure and lyophilized to prepare a powder.

For the hot water extract sample, dry powders of arborvitae leaf and loquat leaf were added 1:10 times with water and extracted at 100° C. for 4 hours in an extractor equipped with a reflux condenser. The extract was obtained in the same manner as the ethanol extract sample to obtain a powder.

The essential oil extract samples were prepared by distillation extraction in hot water at 100° C. for 4 hours, respectively, of dry powders of arborvitae leaf and loquat leaf. As the extraction conditions, a simultaneous distillation and extraction (SDE) apparatus was used. Sodium sulfate was added and filtered to remove moisture contained in the captured essential oil.

A mixture sample was obtained by mixing the ethanol extract and the essential oil extract in equal weights.

Prototype samples were prepared in % by weight as indicated in the table below. 5% ethanol was used as a preservative and solubilizer, and in consideration of use as a mouthwash, 80% ethanol extract of mung bean (prepared in the same manner as above) with surfactant properties was added and used. In order to meet 100% of the total amount of the prototype, sterile water containing 0.0001% of Tween 20, a surfactant, and 0.05% of NaF, which is used for tooth decay prevention, was applied equally to all treatment groups to prepare a prototype.

Prototype composition (%, w/v) division Arborvitae essential oil loquat
essential oil arboretum
ethanol extract loquat
ethanol extract green gram
extract 5% ethanol BP1 0.05 0.05 2.0 2.0 One 4 BP2 0.03 0.03 1.0 1.0 One 4 BP3 - - 1.0 1.0 One 4 BP4 - - 0.1 0.1 One 4

1.2 Antibacterial activity test

Antibacterial activity is, oral microorganisms Streptococcus mutans ( streptococcus mutans ) and other harmful microorganisms Escherichia coli ( E. coli ), Salmonella enterica ( S. enterica ), Vibrio parahaemolyticus ( V. parahaemolyticus ), Bacillus Cereus ( B. cereus ) was targeted.

As a medium, for Streptococcus mutans, BHI (Brain Heart Infusion medium (Bacto BHI, BD, USA) was used, and for other harmful microorganisms, LB medium (Bacto-LB, BD, USA) was used.

Antibacterial activity of the sample was tested using the agar medium diffusion method (disc-agar plate diffusion method). Specifically, the sample was dissolved in diethyl ether at a constant concentration, filtered with a 0.45 μm membrane filter (Milipore, USA), and absorbed into a sterilized filter paper disc (Toyo seisakusho, 8 mm) by 40 μl at various concentrations, followed by nitrogen gas. After removing the ether, it was placed on a test plate medium and incubated for 20 hours in an incubator at 28°C, and then the diameter (mm) of the clear zone around the disc was measured.

1.3 Prototype bacteria count test

The antibacterial activity experiment of harmful microorganisms on the prototype was performed by measuring the number of bacteria using LB medium (other harmful bacteria) and BHI (Brain Heart Infusion) medium (bacterium mutans) by adding the test bacteria solution to the gargle solution.

A mixed strain of S. mutans , E. coli, S. enterica, V. parahaemolyticus, and B. cereus was used for the test solution, and diluted with sterile physiological saline so that the number of viable cells was 1-9.9 × 10 ^{4 CFU/ml was used as the test solution.} did.

0.2 ml of the test bacteria solution was added to 20 ml of the gargle stock solution and left for 0, 30, 60, 90, and 120 seconds. In order to culture the left bacteria, 1 ml of each of the treated samples was taken in liquefied LB and BHI medium at 45-50° C., put in the liquid medium, and put in petri-dish to coagulate. The coagulated petri-dish was cultured at 35°C, tooth decay bacteria for 48 hours, and food harmful bacteria for 24 hours to measure the density of the grown strains.

1.4 Oral bad breath gas analysis test

A total of 10 Dongshin University college students (3 males, 7 females) were prohibited from eating for 30 minutes after lunch. To collect the sample, one minute before the experiment, the mouth was closed and the nose was made to breathe, and then the air in the mouth was gradually filled for 2 minutes using an air bag (polypropylene material) with a volume of 500 ml. Next, rinse the mouth with 20 ml of the gargle solution of the prototype and linsterin for 1 minute, spit out the sample, close the mouth, and slowly fill the mouth with bad breath gas for 2 minutes at intervals of 10, 20, and 30 minutes, respectively, and then seal. The collected air bags were analyzed while being stored at room temperature at 25°C.

The methylmercaptane component was quantified using a gas detector (GV-100S, Gastec, Japan) for the collected bad breath gas. The quantitative method was performed by comparing the measured value of the control group treated with sterile water and the measured value of the prototype treatment (bad breath inhibition rate (%) = 100 x (final value - initial value)/initial value).

1.5 Sensory evaluation

For sensory evaluation, 20 Dongshin University students were used as sensory evaluation agents for irritation degree, color, taste, mouth feel, smell, and preference according to the following 5-point scale method, and each evaluation score was summed. Comparative Example (Control) was carried out with commercially available Listerine (with green tea added).

2. Experimental results

2.1 Antibacterial activity test result

The results of the antimicrobial activity test against mutans bacteria were shown in FIG. 1 for the mixture, and shown in FIG. 2 for the prototype.

Referring to FIG. 1 , whether the mixture sample was a mixture of ethanol extract or a mixture of essential oil extracts, it could be seen that each sample had higher antibacterial activity than a single sample, and the prototype sample showed generally higher antibacterial activity as the amount of sample added increased. In particular, the activity of the prototypes BP1 and B2 was high.

The results of antibacterial activity against other harmful microorganisms are shown in FIGS. 3 to 5 . Figure 3 is the result of the antibacterial activity of the ethanol extract, Figure 4 is the result of the antibacterial activity of the essential oil extract, Figure 5 is the result of the antibacterial activity of the prototype.

Most of the samples showed antibacterial activity to some degree, but especially the essential oil extracts and prototypes BP1 and BP2 showed high activity.

2.2 Prototype bacterial count results

Fig. 6 shows the result of measuring the number of bacteria for the prototype gargle solution. Here, only the prototype BP1 with the highest antibacterial activity was used as the sample. As a result of measuring the number of bacteria, it can be seen that the number of bacteria in the oral cavity before and after gargling was significantly decreased in both the LB medium and the BHI medium.

2.3 Oral bad breath gas analysis test results

7 shows the results of an oral bad breath gas analysis experiment for the mixture sample. Here, only the prototype BP1 with the highest antibacterial activity was used as the sample. BP1 had the effect of removing bad breath similar to the positive control, Listerine.

2.4 Sensory evaluation results

The sensory evaluation results are shown in the table below. Sensory evaluation was also performed with only BP1.

In the case of commercially available Listerine (with green tea added), there were many opinions that it was highly irritating, but the developed prototype BP1 had less irritation and was found to have good overall acceptance. It was confirmed that the prototype CM4 was superior to linsterin.

Claims (5)

A mixture of arborvitae leaf extract and loquat leaf extract as an active ingredient,
The extract is an antibacterial composition against Streptococcus mutans, characterized in that the extract is a mixed solvent extract of water and ethanol or an essential oil extract.
According to claim 1,
The composition is a mouthwash composition or a toothpaste composition, characterized in that the composition.
A mixture of arborvitae leaf extract and loquat leaf extract as an active ingredient,
The extract is a composition for improving dental caries caused by Streptococcus mutans, characterized in that the extract is a mixed solvent extract of water and ethanol or an essential oil extract.
4. The method of claim 3,
The composition is a mouthwash composition or a toothpaste composition, characterized in that the composition.





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