KR102590017B1 - Composition for improving oral condition - Google Patents

Abstract

The composition for removing bad breath of the present disclosure removes hydrogen sulfide and methyl mercaptan, which are volatile sulfides, through a synergistic effect of a combination of one or more of the active ingredients copper gluconate and zinc salt of gluconate, and one or more of propolis extract and black pepper extract. It is quickly removed from the oral cavity, making the bad breath removal effect significantly superior. In addition, the composition for removing bad breath of the present disclosure has a significantly excellent effect on improving bad breath through regulation of the oral microbiome, which reduces the distribution of bad breath-causing microorganisms and increases oral beneficial bacteria when used for a long period of time.

Description

Composition for improving oral condition}

The present disclosure relates to a composition for removing bad breath.

Bad breath is generally caused by acquired systemic diseases or oral diseases such as oral inflammation, or when proteins or food residues in saliva are decomposed by microorganisms in the oral cavity to produce substances that cause bad odor. The main component that causes bad breath is Volatile Sulfide Compounds (VSC). These volatile sulfides include hydrogen sulfide, methyl mercaptan, or dimethyl sulfide. In particular, methyl mercaptan is known to be the main component of bad breath. Additionally, even within the stomach, gases from intestinal decay products are absorbed into the intestinal wall, are transmitted to the lungs through the blood, and are released through exhaled breath.

As a method to suppress such bad breath, there is a method of directly antibacterial or sterilizing oral microorganisms that cause bad breath. Alternatively, by adding fragrance to the oral hygiene composition, bad breath can be eliminated for a short period of time by dulling the sense of smell through masking by fragrance. However, existing oral hygiene compositions of this type only focus on a fragmentary bad breath suppression system that removes the primary cause of bad breath or removes bad breath-generating substances, and their effectiveness also has limitations.

The problem that the present disclosure aims to solve is to provide a composition for removing or improving bad breath that quickly removes hydrogen sulfide and methyl mercaptan that cause bad breath from the oral cavity and suppresses bad breath-causing microorganisms.

In addition, the problem that the present disclosure aims to solve is to provide a composition that improves bad breath through regulation of the oral microbiome, which reduces the distribution of bad breath-causing microorganisms and increases oral beneficial bacteria during long-term use.

As a result of intensive research efforts by the inventors of the present disclosure to solve the above problems, it was found that the combination of one or more of copper gluconate and zinc gluconate, and one or more of propolis extract and cucurbit extract reduces bad breath. It was found that the removal effect for hydrogen sulfide and methyl mercaptan, which cause , increases synergistically. In addition, upon long-term use of the above combination in the oral cavity, microorganisms that cause bad breath are specifically suppressed, and the surprising effect of improving bad breath through regulation of the oral microbiome was discovered, leading to the completion of the present invention.

The present disclosure relates to one or more of copper gluconate and zinc gluconate; and a composition for removing bad breath comprising at least one of copper gluconate and zinc gluconate as an active ingredient. While testing the bad breath removal effect of various ingredients, the inventors of the present disclosure found at least one of copper gluconate and zinc gluconate; It was confirmed that when one or more of the propolis extract and the cucumber extract were used in a specific combination, the hydrogen sulfide and methyl mercaptan removal effects were significantly increased. On the other hand, while other copper salts have a bitter taste and cause rejection, copper gluconate and zinc gluconate have the advantage of having a less bitter taste compared to other copper or zinc salts. In addition, other copper or zinc salts may salt out over time within the formulation, but copper gluconate and zinc gluconate have excellent chelating effects.

In one aspect of the present disclosure, the copper salt of gluconate may be included in an amount of 0.001 to 3% by weight, preferably 0.005 to 1.0% by weight, and more preferably 0.01 to 0.5% by weight, based on the total weight of the composition. If the copper salt of gluconate is less than 0.001% by weight of the total weight of the composition, the effect of removing bad breath is weak, and if it is more than 3% by weight, it may cause a bitter taste and may be unsuitable.

In one aspect of the present disclosure, the zinc salt of gluconate may be included in an amount of 0.001 to 3% by weight, preferably 0.005 to 1.0% by weight, and more preferably 0.01 to 0.5% by weight, based on the total weight of the composition. If the zinc salt of gluconate is less than 0.001% by weight of the total weight of the composition, the effect of removing bad breath is weak, and if it is more than 3% by weight, it may cause a bitter taste and may be unsuitable.

In one aspect of the present disclosure, the composition for removing bad breath may include both copper gluconate and zinc gluconate, in which case the weight ratio of copper gluconate and zinc gluconate is 1:0.1 to 20, preferably It may be 1:1 to 15, more preferably 1:3 to 10, and even more preferably 1:5 to 7 (copper gluconate: zinc gluconate). When the copper gluconate salt and zinc gluconate salt are included in a weight ratio within the above range, the bad breath removal effect can be significantly increased due to the synergistic effect of the combination of the two components.

In one aspect of the present disclosure, the composition for removing bad breath can significantly reduce bad breath caused by hydrogen sulfide and methyl mercaptan by including one or more of propolis extract and black pepper extract, and copper gluconate or zinc gluconate. The bitter or metallic taste caused by can be significantly improved. One or more of the propolis extract and the cucumber extract may be included in an amount of 0.001 to 3% by weight, preferably 0.05 to 1.0% by weight, more preferably 0.1 to 0.5% by weight, based on the total weight of the composition, and the components are included in the above content. The effects of reducing bad breath and improving bitter taste can be significantly increased.

In one aspect of the present disclosure, in the composition for removing bad breath, one or more of copper gluconate and zinc gluconate; and the weight ratio of one or more of the propolis extract and the cucumber extract is 1:0.1 to 10, preferably 1:0.3 to 5, more preferably 1:0.5 to 3, even more preferably 1:0.7 to 1 (copper gluconate salt and It may be one or more of the zinc salts of gluconate: one or more of the propolis extract and the cucurbit extract. When the above ingredients are included in a weight ratio within the above range, the bad breath removal effect can be significantly increased due to the synergistic effect of the combination of each ingredient.

In one aspect of the present disclosure, the composition for removing bad breath may preferably include copper gluconate, zinc gluconate, and black pepper extract. Also preferably, the composition for removing bad breath may include copper gluconate, zinc gluconate, and propolis extract. Also preferably, the composition for removing bad breath may include zinc gluconate, leaf extract, and propolis extract. When the above combination is used, the bad breath removal effect can be increased more significantly.

In one aspect of the present disclosure, the composition for removing bad breath has a significantly excellent inhibitory effect on bad breath-causing bacteria, and thereby reduces volatile sulfides generated by bad breath-causing bacteria, thereby significantly improving the bad breath removal effect. . The bad breath-causing bacteria include, but are not limited to , Streptococcus mutans , Porphyromonas gingivalis , or Fusobacterium nucleatum . Preferably, the composition for removing bad breath of the present disclosure may have a significantly excellent inhibitory effect on Streptococcus mutans, Porphyromonas gingivalis, or Fuzobacterium nucleatum.

In one aspect of the present disclosure, the composition for removing bad breath may be a pharmaceutical composition for preventing or treating bad breath. In this case, the composition for removing bad breath includes at least one of copper gluconate and zinc gluconate; and an effective amount of one or more of propolis extract and cucurbita extract, along with one or more pharmaceutically acceptable carriers, excipients, or diluents. The effective amount refers to an amount sufficient to prevent, improve, and treat bad breath. This may change appropriately depending on the severity of symptoms of bad breath disease, the patient's age, weight, health condition, gender, administration route, and treatment period. The carriers, excipients, and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, polyvinyl methylcellulose. It may include rolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil.

Additionally, the pharmaceutical composition for removing bad breath may be formulated using methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. Dosage forms may be in the form of powders, granules, tablets, emulsions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, or sterile powders. Additionally, the composition for removing bad breath of the present disclosure may be administered in combination with a known compound that has the effect of preventing, improving, or treating oral disease or bad breath.

In one aspect of the present disclosure, the composition for removing bad breath may be a quasi-drug composition for preventing, improving, or eliminating bad breath. When the composition for removing bad breath of the present disclosure is used for quasi-drug purposes, the active ingredient with bad breath removing activity can be added as is or used together with other quasi-drugs or quasi-drug ingredients according to a conventional method. The mixing amount of the active ingredients can be appropriately determined depending on the purpose of use. The quasi-drug composition for removing bad breath is not particularly limited in its formulation, and can be formulated in various forms as quasi-drugs known in the art that have a bad breath removal effect. In addition, the quasi-drug composition for removing bad breath may include common auxiliaries or carriers such as fluorine, thickeners, stabilizers, solubilizers, vitamins, pigments, or fragrances, depending on the formulation or purpose of use.

In one aspect of the present disclosure, the composition for removing bad breath may be a cosmetic composition for preventing, improving, or removing bad breath. The cosmetic composition for removing bad breath can be formulated in various forms as cosmetics known in the art, and additional ingredients can be arbitrarily selected and appropriately mixed depending on the formulation and purpose of use. For example, common ingredients such as thickeners, surfactants, moisturizers, abrasives, sweeteners, pH adjusters, preservatives, binders, wetting agents, foaming agents, stabilizers, solubilizers, vitamins, pigments or fragrances may be included in the cosmetic composition. there is.

In one aspect of the present disclosure, the composition for removing bad breath may be a food composition for preventing, improving, or removing bad breath. The food composition for removing bad breath may include food additives that are foodologically acceptable in addition to the active ingredients. The food supplement refers to a component that can be added to food for preservation, and can be appropriately selected and used by a person skilled in the art as it is added to manufacture each type of health functional food. Examples of food supplements include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, complexing agents and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloids. Thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, or carbonating agents used in carbonated beverages are included, but the types are not limited thereto.

Additionally, the food composition may include health functional foods. In this disclosure, health functional food refers to a food group or food composition that has added value to the food by using physical, biochemical, biotechnological methods, etc. to function and express the function of the food for a specific purpose, such as regulation of biological defense rhythm, disease prevention, and other functions. It refers to food that has been designed and processed to sufficiently express body regulation functions related to recovery, etc. in the living body. Health supplement food refers to food that has a more active health maintenance or promotion effect than regular food and is intended for health supplementation. In some cases, the terms functional food, health food, and health supplement food are used interchangeably. Specifically, the health functional food is a food prepared by adding the composition of the present disclosure to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspending it, and consuming it may cause certain health problems. It means bringing about an effect, but unlike regular drugs, it has the advantage of not having any side effects that may occur when taking the drug for a long time since it is made from food. The health functional food of the present disclosure can be manufactured by mixing known additives with other appropriate auxiliary ingredients that can be included in the food, according to the selection of a person skilled in the art. Examples of foods that can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, or There are vitamin complexes, etc., and they can be prepared by adding the composition according to the present disclosure to juice, tea, jelly, juice, etc. prepared using the composition as the main ingredient.

In one aspect of the present disclosure, the composition for removing bad breath may be a pet food composition for preventing, improving, or removing bad breath in companion animals. The type of the feed is not particularly limited, and feeds commonly used in the art can be added and used. In this case, the feed composition of the present disclosure can serve as a feed additive. Non-limiting examples of the feed that can be added include vegetable feed such as grains, roots and fruits, food processing by-products, algae, fiber, pharmaceutical by-products, oils and fats, starches, cucurbits or grain by-products; Examples include animal feed such as proteins, inorganic substances, fats and oils, minerals, oils and fats, single-cell proteins, zooplanktons, or food. These may be used alone or in combination of two or more types. The feed composition can be appropriately used depending on the type, age, and types of other feed ingredients of the companion animal to be administered.

Additionally, the present disclosure provides an oral product containing the composition for removing bad breath. one or more of copper gluconate and zinc gluconate; and the composition for removing bad breath of the present disclosure, which contains one or more of propolis extract and cucurbita extract as active ingredients, is an oral composition that can be contained in products generally manufactured and sold for oral health, and can be applied to the oral cavity. There are no restrictions on products for use. For example, the oral products may include, but are not limited to, toothpaste, oral spray, mouthwash, oral ointment, mouthwash, gargling product, oral strip (film), or gum. The oral product of the present disclosure may be in the form of liquid, solid, suspension, gel, or aerosol, but is not limited thereto. In order to apply the oral product of the present disclosure, appropriate additives or carriers can be additionally included in the composition for removing bad breath, and the additives and carriers can be appropriately selected and combined by those skilled in the art. You can.

Additionally, the present disclosure relates to one or more of copper gluconate and zinc gluconate; and treating the oral cavity with a composition for removing bad breath containing at least one of propolis extract and cucurbit extract as an active ingredient. The composition used in the method for removing bad breath is the same as described for the composition for removing bad breath.

Additionally, the present disclosure relates to one or more of copper gluconate and zinc gluconate; and a method for producing a composition for removing bad breath comprising at least one of propolis extract and cucurbit extract as an active ingredient. The above manufacturing method can be performed through a conventional method in the technical field to which the present invention pertains.

All ingredients described in the present disclosure are preferably subject to relevant laws and regulations of Korea, China, the United States, Europe, Japan, etc. (e.g., Regulations on Cosmetic Safety Standards, etc. (Korea), Cosmetic Safety Technical Regulations (China), etc. It does not exceed the maximum usage level specified in the Food Code (Korea), Food Additives Code (Korea), and Health Functional Food Code (Korea). That is, preferably, the cosmetic, pharmaceutical, food, pet food or pharmaceutical composition according to the present disclosure contains the ingredients according to the present disclosure within the content limit permitted by the relevant laws and regulations of each country.

The composition for removing bad breath of the present disclosure includes at least one of the active ingredients copper gluconate and zinc gluconate; And through the synergistic effect of the combination of one or more of the propolis extract and the cucumber extract, volatile sulfides such as hydrogen sulfide and methyl mercaptan are quickly removed from the oral cavity, and the effect of removing bad breath is significantly excellent.

In addition, the composition for removing bad breath of the present disclosure has a significantly excellent effect on improving bad breath through regulation of the oral microbiome, which reduces the distribution of bad breath-causing microorganisms and increases oral beneficial bacteria when used for a long period of time.

Figure 1 shows the results of an experiment on the reduction of volatile sulfide when treated with copper salt or zinc salt in Experimental Example 1.
Figure 2 shows the results of testing the synergistic effect of the combination of copper salt and zinc salt in reducing volatile sulfide in Experimental Example 2.
Figure 3 shows the results of testing the inhibitory effect of volatile sulfide depending on the type of bad breath-causing bacteria when using copper gluconate, zinc gluconate, or a mixture thereof in Experimental Example 3.

Hereinafter, to aid understanding of the present invention, it will be described in detail through examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more completely explain the present invention to those with average knowledge in the field to which the present invention pertains. The raw materials used in the present invention were purchased from a typical raw material company.

Experimental Example 1: Volatile sulfide reduction effect depending on the type of copper salt or zinc salt

This experiment was performed in vitro to evaluate the effect of copper salts or zinc salts on suppressing and eliminating bad breath. The experimental method was to prepare artificial bad breath (volatile sulfide), treat it with copper salt or zinc salt, and measure the bad smell using an OralChroma device. The artificial bad breath (volatile sulfide) was prepared by mixing NaSH (0.001%) and NaSCH ₃ (0.0002%) in a ratio of 7:3.

Looking at the experimental results shown in Figure 1, zinc salt showed a hydrogen sulfide removal effect of more than 60% and a methyl mercaptan removal effect of more than 25%. Meanwhile, copper salt (copper gluconate salt) showed more than 70% of both the hydrogen sulfide removal effect and the methyl mercaptan removal effect.

Experimental Example 2: Synergistic effect in reducing volatile sulfide when combining copper salt and zinc salt

In the same manner as Experimental Example 1, the synergistic effect of the combined treatment of copper salt and zinc salt on the inhibitory effect of volatile sulfide was tested. Looking at the experimental results shown in Figure 2, when treated with a combination of copper salt and zinc salt at a ratio of 1:6, both the hydrogen sulfide removal effect and the methyl mercaptan removal effect were increased to more than 90%. Among them, when treated with a combination of copper gluconate and zinc gluconate, the methyl mercaptan removal effect was the best.

Experimental Example 3: Inhibition of volatile sulfide produced by bad breath-causing bacteria

In this experimental example, copper salt, zinc salt, or a mixture thereof was added to the bad breath-causing bacteria Streptococcus mutans , Porphyromonas gingivalis , or Fusobacterium nucleatum . The effect of suppressing volatile sulfides during treatment was tested. In this experiment, volatile sulfides were measured using Headspace vials and OralChroma instruments.

Looking at the experimental results shown in Figure 3, when treated with a combination of copper salt and zinc salt, compared to treatment with zinc salt or copper salt alone, the effect of suppressing volatile sulfide on the bad breath-causing bacteria was significantly increased. Preferably, when Porphyromonas gingivalis was treated with a mixture of copper gluconate and zinc gluconate in a ratio of 1:6, the volatile sulfide suppression effect was significantly increased.

Experimental Example 4: Minimum inhibitory concentration (MIC) for bad breath-causing bacteria

For the three types of bad breath-causing bacteria in Experimental Example 3, the minimum inhibitory concentration (MIC) was evaluated when copper gluconate and zinc gluconate were mixed at a ratio of 1:6.

Sample bacteria MIC(%) Raw material CuG/ZnG (1/6ratio) S. mutans 0.0625 CuG/ZnG (1/6ratio) P. gingivalis 0.0040 CuG/ZnG (1/6ratio) F. nucleatum 0.0010 oral products Control toothpaste (with NaF) P. gingivalis 25 CuG/ZnG toothpaste P. gingivalis 12.5

Looking at the evaluation results shown in Table 1 above, the minimum inhibitory concentration for Porphyromonas gingivalis or Puzobacterium nucleatum was lower than that for Streptococcus mutans. Additionally, when treated with toothpaste containing copper gluconate and zinc gluconate, the minimum inhibitory concentration against Porphyromonas gingivalis was found to be half that of the control toothpaste (containing NaF). From these experimental results, it can be seen that the combined treatment of copper gluconate and zinc gluconate significantly inhibits the growth of bad breath-causing bacteria, showing an excellent bad breath suppressing effect.

Experimental Example 5: Effect of removing bad breath when adding cucurbit extract, propolis extract, or mixtures thereof

one or more of copper gluconate and zinc gluconate; And the effect of removing bad breath was evaluated when using a composition for removing bad breath containing one or more of propolis extract and cucurbit extract as active ingredients. According to the compositions shown in Table 2 below, the mouthwash compositions of Comparative Examples 1 to 5 and Examples 1 to 4 were prepared.

The effect of suppressing bad breath substances was performed according to the saliva fermentation method. First, around 30 mL of fasting saliva from healthy adults was collected and then centrifuged at room temperature for 15 minutes at a speed of 1,740 g. The supernatant was removed, the precipitate was collected, washed twice with sterile distilled water, and dispersed in sterile distilled water 10 to 20 times the amount of the precipitate to obtain a suspension. 250 μl of 6mM cysteine, 250 μl of 6mM methionine, and 250 μl of sample were added to 500 μl of the precipitation suspension, and then reacted at 37°C for 4 hours, and then the amount of hydrogen sulfide and methyl mercaptan generated was measured using Oral Chroma.

Raw materials Comparative Example 1 Comparative example 2 Comparative Example 3 Comparative example 4 Comparative Example 5 Example 1 Example 2 Example 3 Example 4 Floxamer 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 polyoxyethylene hydrogenated castor oil) One One One One One One One One One Spices 0.35 0.35 0.35 0.35 0.35 0.35 0.35 0.35 0.35 Saccharin sodium hydrate 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 sucrose 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03 sodium fluoride 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 citric acid 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 sodium citrate 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03 Na Benzoate 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 glycerin 5 0.3 5 5 5 5 5 5 5 Cucurbit Extract 0.325 - - - - 0.15 - 0.05 0.05 Propolis Extract - 0.325 - - - - 0.15 0.1 0.1 Zn-gluconate - - 0.325 - 0.2 0.15 0.15 0.175 0.15 Cu gluconate - - - 0.325 0.125 0.025 0.025 - 0.025 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Comparative Example 1 Comparative example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Example 1 Example 2 Example 3 Example 4 Hydrogen sulfide removal rate (%) -321 -383 35.6 45 48 88 93.3 92 95.2 Methyl mercaptan removal rate (%) 5.3 2.5 5.5 55 65 93.5 93 92 98.9 Suppression rate of bad breath substances by saliva fermentation method (%) 15.4 22.3 36.5 61 56 94 94 91 97 Is there a metallic taste? - - Good strong bitter taste strong bitter taste Good Good Good Good

From the experimental results shown in Table 3, it can be seen that the hydrogen sulfide and methyl mercaptan removal rates of Examples 1 to 4 are significantly superior to those of Comparative Examples 1 to 5. At least one of copper gluconate and zinc gluconate, and It can be seen that the combination of one or more of the propolis extract and the cucurbit extract has a synergistic effect in removing hydrogen sulfide and methyl mercaptan. In addition, Examples 1 to 4 confirmed that the effect of suppressing bad breath substances was significantly increased, and the metallic taste (bitterness) caused by copper salts was also reduced.

In addition, the toothpaste compositions of Comparative Examples 6 to 10 and Examples 5 to 8 were prepared according to the compositions shown in Table 4 below.

Raw material Comparative Example 6 Comparative Example 7 Comparative example 8 Comparative Example 9 Comparative Example 10 Example 5 Example 6 Example 7 Example 8 glycerin 2 2 2 2 2 2 2 2 2 Sorbitol 45 45 45 45 45 45 45 45 45 sodium fluoride 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Sodium pyrophosphate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 spandex 80 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Silica (for polishing) 14 14 14 14 14 14 14 14 14 C.M.C. One One One One One One One One One xanthan gum 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Lauryl sulfate 2 2 2 2 2 2 2 2 2 Spices 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Propolis Extract 0.325 - - - - 0.15 - 0.05 0.05 Cucurbit extract - 0.325 - - - - 0.15 0.1 0.1 Zn-gluconate - - 0.325 - 0.2 0.15 0.15 0.175 0.15 Cu gluconate - - - 0.325 0.125 0.025 0.025 - 0.025 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Comparative Example 6 Comparative Example 7 Comparative example 8 Comparative Example 9 Comparative Example 10 Example 5 Example 6 Example 7 Example 8 Hydrogen sulfide removal rate (%) -197 -146 15 55.3 55 94.5 93.2 93.2 96.5 Methyl mercaptan removal rate (%) 6.4 28 3.2 45.2 60 92.3 93.1 92.2 94.5 Suppression rate of bad breath substances by saliva fermentation method (%) 10.2 43.2 38 42 75 96 97.3 96 97.3 Is there a metallic taste? - - Good strong bitter taste strong bitter taste Good Good Good Good

From the experimental results shown in Table 5, it can be seen that the hydrogen sulfide and methyl mercaptan removal rates of Examples 5 to 8 are significantly superior to those of Comparative Examples 6 to 10. At least one of copper gluconate and zinc gluconate; It can be seen that the combination of one or more of the propolis extract and the cucumber extract has a synergistic effect in removing hydrogen sulfide and methyl mercaptan. In addition, Examples 5 to 8 confirmed that the effect of suppressing bad breath substances was significantly increased, and the metallic taste (bitterness) caused by copper salts was also reduced.

Experimental Example 6: Clinical evaluation of bad breath after using mouthwash and toothpaste

A clinical evaluation of bad breath was performed after using the mouthwash composition of Comparative Example 1 or Example 4. The initial degree of bad breath was measured using sensory (Rosenberg scale) and instrumental methods (Halimeter, Oral Chroma) in healthy people who felt they had bad breath in an empty stomach. Afterwards, each experiment participant was asked to use the mouthwash, and the level of bad breath 30 minutes and 1 hour after use was measured using the same method as before use. I gargled with 10mL of the mouthwash, then spit it out and did not rinse it with water. After using the product, intake of water or food was restricted until the measurement was completed. The clinical evaluation results of the mouthwash composition are shown in Table 6 below.

product Sensory evaluation (strength, 0-5)* Average Halimeter (VSCs) Oral Chroma (Total_H ₂ S, CH ₃ SH) Before use 30 minutes later 1 hour later Before use 30 minutes later 1 hour later Before use 30 minutes later 1 hour later Comparative Example 1 4.4 3.4 3.8 368.2 335.5 397.3 804.6 753.2 683.7 Example 4 4.6 1.9 2.3 285.3 98.3 114.6 908.4 64.2 39.6

* Sensory evaluation is on a 5-point scale, with 5 being a very strong bad breath, and the bad breath evaluation unit is ppb.

In addition, a clinical evaluation of bad breath was performed after using the toothpaste composition of Comparative Example 6 or Example 8. The clinical evaluation of the toothpaste composition was performed in the same manner as the clinical evaluation of the toothpaste, except that about 2 g of toothpaste was applied to a toothbrush, brushing the teeth for 1 minute, and rinsing thoroughly with 1 cup (about 200 mL) of water. The clinical evaluation results of the toothpaste composition are shown in Table 7 below.

product Sensory evaluation (intensity, 0-5) Average Halimeter (VSCs) Oral Chroma (Total_H ₂ S, CH ₃ SH) Before use 30 minutes later 1 hour later Before use 30 minutes later 1 hour later Before use 30 minutes later 1 hour later Comparative Example 6 4.7 4.2 4.2 421.2 457.5 382.4 854.8 806.2 660.5 Example 8 4.5 2.3 2.7 357.9 187 135 676.7 130.2 103

Considering the experimental results shown in Tables 6 and 7 above, one or more of copper gluconate and zinc gluconate; And when using an oral product formulation of a mouthwash composition or a toothpaste composition containing one or more of propolis extract and cucurbita extract, it can be seen that volatile sulfides are suppressed and the bad breath removal effect is significantly excellent.

Claims (14)

Streptococcus mutans , Porphyromonas gingivalis , or Fusobacterium nucleatum , comprising copper gluconate salt, zinc salt gluconate, and propolis extract as active ingredients. ) Oral composition for antibacterial use. According to claim 1, wherein the copper gluconate salt is Streptococcus mutans ( Streptococcus mutans ), Porphyromonas gingivalis ( Porphyromonas gingivalis ) or Puzobacterium nucleatum ( Antibacterial oral composition against (Fusobacterium nucleatum ) bacteria. The method of claim 1, wherein the zinc gluconate salt is Streptococcus mutans , Porphyromonas gingivalis , or Puzobacterium nucleatum (0.001 to 3% by weight based on the total weight of the composition). Antibacterial oral composition against (Fusobacterium nucleatum ) bacteria. The method of claim 1, wherein the propolis extract is Streptococcus mutans , Porphyromonas gingivalis , or Fusobacterium , which is 0.001 to 3% by weight based on the total weight of the composition. nucleatum ) Oral composition for antibacterial use against bacteria. The method of claim 1, wherein the weight ratio of the copper gluconate salt and the zinc gluconate salt is 1:0.1 to 20 (copper gluconate salt: zinc gluconate salt), Streptococcus mutans , Porphyromonas gingivali An oral composition for antibacterial use against Porphyromonas gingivalis or Fusobacterium nucleatum . The method of claim 1, wherein the copper gluconate salt and the zinc gluconate salt; And the weight ratio of the propolis extract is 1:0.1 to 10 (copper gluconate and zinc gluconate: propolis extract), Streptococcus mutans , Porphyromonas gingivalis , or Peugeot. Antibacterial oral composition against Bacterium nucleatum ( Fusobacterium nucleatum ) bacteria. The method of claim 1, wherein the antibacterial oral composition inhibits or removes volatile sulfide, Streptococcus mutans , Porphyromonas gingivalis , or Fusobacterium nucleatum . Oral composition for antibacterial use. delete The method of any one of claims 1 to 7, wherein the composition is a quasi-drug composition, Streptococcus mutans , Porphyromonas gingivalis , or Puzobacterium. Nucleatum ( Fusobacterium nucleatum ) Oral composition for antibacterial use. 8. The method of any one of claims 1 to 7, wherein the composition is a cosmetic composition, Streptococcus mutans , Porphyromonas gingivalis , or Puzobacterium new. Antibacterial oral composition against Fusobacterium nucleatum bacteria. 8. The method according to any one of claims 1 to 7, wherein the composition is a pharmaceutical composition. Streptococcus mutans , Porphyromonas gingivalis or Puzobacterium Nucleatum ( Fusobacterium nucleatum ) Oral composition for antibacterial use. 8. The method according to any one of claims 1 to 7, wherein the composition is a food composition, Streptococcus mutans , Porphyromonas gingivalis , or Puzobacterium new. Antibacterial oral composition against Fusobacterium nucleatum bacteria. 8. The method according to any one of claims 1 to 7, wherein the composition is a pet food composition. Streptococcus mutans , Porphyromonas gingivalis or Fuzobactere. Antibacterial oral composition against Fusobacterium nucleatum bacteria. Antibacterial oral use against Streptococcus mutans , Porphyromonas gingivalis or Fusobacterium nucleatum bacteria, comprising the antibacterial oral composition according to claim 1. product.