KR20170079912A - Feed additives for abalone - Google Patents

Abstract

The present invention is a new alternative protein source for replacing expensive fish meal for overturning production. It is a new feed substitute for environmentally friendly abalone using seaweed as a substitute feed additive, and substitutes spirulina meal and wheat flour for fermented seaweed extract, In addition to supplementing insufficient nutrients, it is a composition for growth promotion having effects of improving feed efficiency, increasing weight gain, activating digestive enzyme secretion, increasing immunity, improving reproductive productivity, antibiosis and antioxidation. The present invention relates to an abalone feed additive containing an economical high quality fermented wakame extract as an active ingredient which promotes the growth of the abalone and improves the growth rate by increasing the resistance to changes in water quality and diseases.

Description

{Feed additives for abalone} containing an extract of fermented seaweed and fermented plant complex as an active ingredient,

The present invention relates to an abalone feed additive comprising an extract of fermented marine algae as an active ingredient,

More specifically, as an eco-friendly abalone substitute feed additive using seaweed, Spirulina fish meal and wheat flour were replaced with fermented seaweed extract, respectively. In addition to supplementing insufficient nutrients in the conventional feed composition for overturning of basic nutrients, improvement of feed efficiency, As a composition for growth promotion having effects of activation of secretion, improvement of immunity, improvement of reproductive productivity, antimicrobial action, and antioxidation, the seaweed fermentation extract is mixed and formed to promote the growth of abalone as well as to increase the resistance to changes in water quality and diseases The present invention relates to an abalone feed additive comprising an economical high quality fermented wakame extract as an active ingredient, which can promote the growth of abalone and improve the growth rate.

Abalone is a high-value food cultivated from ancient times as a high-priced preference food with industrial prospects. Abalone's body is traditionally high-quality fish food and inner shell surface with iridescent pearl luster is used as a craft for high-grade mother-of-pearl. And abalone farming is also used as a seed for pearl farming.

Recently, abalone farming industry in Korea has grown greatly, but the disease of abalone during farming has been a crucial factor for hindering development of abalone farming industry.

In recent years, abalone farming has mainly been carried out by artificial nursery production, in which the seedlings are discharged to the reef area and re-harvested in the size of the commodity. The abalone is cultivated in the aquaculture tank as a compound feed to improve the survival rate and growth rate of abalone. The most important consideration in the aquaculture is proper water temperature and sufficient food supply, and it is possible to maintain the proper water temperature of abalone at 18 ~ 22 ℃ in aquaculture tank style. Has become an important factor.

Stable supply of feed is becoming a big obstacle in abalone farming. In other words, abalone cultured fish are mainly used for nourishing food such as seaweed and raw seaweed, and they supply dried seaweed, kelp, and salted seaweed in the summer season.

In the abalone diet of natural feed, feed is the main factor that determines the success of the aquaculture. It not only grows abalone but also affects water pollution and economic efficiency. When nourishing food is used for natural food such as camphor and raw kelp It is reported that the growth effect is lower than that of the compound feed because the production supply is unstable due to the timing and local circumstances and the price fluctuation is severe and the main nutrient content is lower than the required amount for normal growth of the abalone.

Especially, fishermen who cultivate abalone are using natural food such as seaweed, parasites, and kelp as food for breeding, and they supply dried seaweeds and dried kelp in summer, which is a limiting factor for systematic aquaculture.

Currently, abalone is dependent on seaweed and kelp. It needs about 600,000 tons of seaweed for the production of 30,000 tons of abalone, but the production of algae is not much bigger than that for abalone production.

In other words, the supply of natural food is not only unstable but also has a disadvantage of fluctuating price and it is also reported that the growth rate is lower than that of mixed nutrients which have various nutrients required for aquaculture. Therefore, it is urgently required to supply alternative feeds for abalone cultivation which can produce more economically and at the same time, growth of abalone is advantageous

Seaweeds are classified into red algae, green algae and brown algae according to their color, photosynthetic pigment. Brown algae include seaweed, kelp, tortoise, mackerel, tortoiseshell, crab, and seal. Red algae refers to such things as Kim, mugwort, and squid. The green algae have something like flower buds, parasites, and hearing.

In addition, the availability of seaweeds and the excellent pharmacological effects such as physiologically active substances have been revealed and the interest of modern people in seaweeds is increasing.

Sea mustard (Undaria pinnatifida) is a representative brown algae belonging to the sea tangle mackerels. It is one of the most important food resources in the world in 2000, accounting for 33% of aquatic aquatic production and 57% of aquaculture.

Seaweed is mainly composed of proteins, carbohydrates and minerals except moisture. The amino acid composition of the seaweed is similar to that of soybean and fish meat protein, so it is nutritionally superior. Minerals are rich in calcium, magnesium, sulfur, iodine and iron And the vitamin A content is 5550 IU, which is characterized by being contained in a larger amount than other food ingredients. It is also known as an alkaline health food, a food containing many physiologically active substances, and a variety of nutritional ingredients

As described above, the seaweed contains dietary fiber, iodine, iron, potassium, calcium and the like, and is highly likely to be used as an alternative agent for antibiotics used for promoting growth of feeds due to various functions other than antioxidant, antibacterial and anticancer effects .

Accordingly, the present inventors have made efforts to maximize the efficiency of feed, and as a result, developed an abalone feed additive containing an active ingredient of fermented wakame extract.

The inventors of the present invention have developed an abalone feed additive containing the fermented marine algae extract as an active ingredient as described above, thereby improving the adaptability to changes in the breeding environment of the abalone, growing the abalone in a healthier manner without infecting the disease during the overturning period, The feed efficiency can be maximized through an increase in the feed intake rate, thereby reducing the economic loss due to overturning of the abalone, and also achieving the environment-friendly manner by reducing the pollution of the marine environment. will be.

As described above, the overturned feeds of the prior art have various problems, and as a green abalone feed additive using the fermented marine algae extract, it is possible to replace the spirulina fish meal and the wheat flour with the fermented seaweed extract, In addition to supplementing nutrients, it also improves feed efficiency, increases weight gain, activates digestive enzyme secretion, increases immunity, improves breeding productivity, antimicrobial activity, and antioxidant. The present invention solves the technical problem of developing economical high-quality overturned feed additive which can increase the resistance to water quality and disease, thereby promoting the growth of abalone and improving the growth rate.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,

An overturned feed additive comprising seaweeds,

The abalone feed additive containing the fermented seaweed extract as an active ingredient,

After immersion and washing, the vacuum-dried seaweed is finely pulverized, and then the fermenting microorganism bacillus subtilis (Bacillus subtilis) is used to perform the fermentation microorganism action for 20 to 30 hours at a temperature of 20 ° C to 40 ° C Fermented seaweed extract obtained by fermenting at low temperature, and extracting a plant herb complex, which is a natural herbal medicine herb extract, bokbunja extract, white mulberry extract, early stage extract and fermented extract of Saururus chinensis extract as effective ingredients in one or more equivalent ratios, 1 < / RTI > as an active ingredient. The present invention also provides an abalone feed additive comprising the fermented marine algae extract as an active ingredient.

As described above,

The abalone feed additive comprising the fermented marine algae extract of the present invention as an active ingredient has not only a nutritional balance but also a nutrient and vitamin inorganic substance which are insufficient in conventional abalone feeds, as well as feed efficiency improvement, increase in body weight gain, The present invention relates to a composition for promoting growth which is effective for improving growth, promoting growth of abalone by enhancing immunity of abalone and promoting digestion by enhancing survival rate and growth rate. Which can be used to grow healthy abalone, thus maximizing the efficiency of the feed

The present invention can replace Spirulina fish meal and wheat flour as eco-friendly abalone feed additive using seaweed recycled wasteweed as an economical additive, thereby reducing the cost of protein source of abalone feed Abalone's price competitiveness will increase.

That is, the present invention can meet the nutritional requirement of abalone required for rapid growth by adding fermented seaweed as an algae extract and replacing an animal protein with a vegetable protein, thereby enhancing growth and affecting body composition, To improve the adaptability to the environmental change of the abalone, or to increase the bioactivity of the active ingredient against the abalone, thereby improving the utilization efficiency of the feed as a whole.

Particularly, the feed additive of the present invention is a feed additive capable of replacing antibiotics using natural products, and includes a fermented seaweed fermented product obtained by adding a Bacillus subtilis strain having alginic acid decomposition ability and antibacterial activity to a feed composition containing fermented seaweed, When the feed is fed to the abalone, the bioavailability of the active ingredients contained in the feed is increased to increase the amount of the abalone to be groomed, and the antimicrobial substance is contained, thereby improving the immunity against fish pathogenic bacteria and improving the abalone growth rate and survival rate Can

The feed prepared as described above can have an effect of increasing the immunity and improving the growth ability by containing the active ingredient of the seaweed, and it is possible to recycle the previously abandoned seaweed by-products

In order to achieve the above object, the present invention will be described in detail.

In the overturned feed additive comprising the seaweed of the present invention,

The abalone feed additive containing the fermented marine leaf extract as an active ingredient is prepared by immersing and washing, then finely pulverizing the seaweed subjected to low-temperature vacuum drying, and then pulverizing the fermented marine bacillus subtilis (Bacillus subtilis, Bacillus subtilis) Temperature fermented seaweed extract for 20 to 30 hours under the action of fermentation microorganism for 20 to 30 hours under the action of fermentation microorganism, and extracting the natural herbal medicine raw extract, bokbunja extract, white sardine extract, Which comprises a fermented marine algae extract obtained by mixing a plant complex extract containing an effective ingredient at a ratio of 10: 1 in terms of a total weight ratio, as an active ingredient.

The composition of the present invention, which has been mixed and compounded as described above, is preferably added as an essential ingredient to be normally added as an abalone feed additive considering the physiology of abalone, and it is preferable to add 5 to 25% by weight in the total weight ratio in the abalone- Functional Feed Additive Composition The composition contains protein, carbohydrate, lipid and fatty acid, minerals as various nutrients, and various vitamins in a proper amount according to the overturning growth stage to provide all necessary nutrients for abalone cultivation, And increased immunity and formation of eco-friendly feeds close to the most natural food with optimal taste and nutrients, reducing underwater corruption at high water temperatures, preventing feed loss and reducing water pollution, enabling eco-friendly form of healthy abalone .

When the feed additive of the present invention is added to the overturned combination feed, if the content of the feed additive is too small, the effect of addition is small and if the added amount is too large, it becomes uneconomical and not preferable. Most preferred is the addition of the functional feed additive of the present invention By weight based on the total weight of the composition.

The present invention ferments seaweed, which is a brown algae, with bacillus subtilis (Bacillus subtilis) as a fermenting microorganism, to obtain fucoxanthin as an effective active ingredient in a high yield. These microorganisms are exemplified for illustrative purposes only and are useful A variety of known microorganisms can be used, and the microorganisms can be obtained in a natural state, a commercially available one, or a commercially available microorganism from a host organism, preferably Bacillus subtilis) is preferred.

In the present invention, the molecular weight of the fuco xanthine is very high, and it is difficult to absorb the fucosanthin at a low molecular weight of less than 10,000. Therefore, the fucosantine has low molecular weight through low temperature microbial fermentation which minimizes the destruction of nutrients desirable.

In the present invention, the seaweed is cleaned and then pulverized. Purified water is a nano bubbling water that promotes the high-purity disinfection of microorganisms and the decomposition of refractory organic matter. Purified water uses nano bubbling water having excellent washing and disinfection efficiency as fresh water To remove the toxicity inherent to kelp (arine taste, bitter taste, salty taste, etc.).

The purified water of the present invention is a nano-sized bubble that can be present in the liquid for a long period of time, and can perform a complex action such as sterilization and deodorization as well as purification of water The sterilization of the high-purity microorganisms and the disinfection of the degradable organic matters are further performed by the strong sterilizing power.

The drying of the seaweed is performed by a low-temperature vacuum drying step. In the low-temperature vacuum drying step, the seaweed is put into a low-temperature vacuum dryer and dried until the moisture content reaches 10% or a far-infrared drying method may be used.

It is a drying method that activates the cell tissue and makes the taste and color unchanged in the drying of the seaweed. It can use the freeze drying method or the far infrared ray drying method so that the nutrient destruction is minimized. When the far infrared rays are irradiated, By activating the material, it is possible to maximize the antioxidant power, and at the same time, the strong sterilization and purification of far infrared rays can remove heavy metals and harmful substances.

In addition, the crushing of the seaweed is finely pulverized into fine powder of 100 ~ 300 mesh to maintain the incense, color and nutrients inherent in seaweed. The fiber is separated into finely divided powder so that the nutrients are not destroyed. It is preferable to use an aerodynamic grinder to form the powder in the form of a processed powder in which the nutrients are not destroyed by pulverization.

As a result, the present invention can obtain the extract at a high yield by a relatively simple procedure while minimizing the loss of fucoxanthin as an active ingredient from seaweed, which is a brown algae.

Also, the natural herbal medicine which is included in the feed additive of the present invention as an active ingredient and produces safe abalone by replacing the antibiotic, is a natural herb medicine which comprises five kinds of natural herbal medicine extracts of bamboo extract, bokbunja extract, The content of the plant complex extract is not limited, but may be included in the same amount as the total weight of the plant complex extract composition. The extracts of the plant extract, bokbunja extract, white sachet extract, %.

As used herein, the term 'extract' has the meaning of being used as a crude extract in the art, and the composition ratio of each ingredient can be added or subtracted within a usual range when the feed additive of the present invention is manufactured.

In the present invention, the plant complex extract of the present invention is a composition for promoting abalone growth comprising at least one of natural extract, crude extract, bamboo extract, early extract, saururus chinensis extract as an active ingredient, The present invention provides a method for improving the survival rate and the growth rate of abalone to improve the ability to infect and improve healthy abalone. In particular, in the present invention, among a plurality of plant- ) It is composed of a herb medicine which increases the production amount and is formed at an optimum compounding ratio. It stimulates bioprotein synthesis and cell differentiation by IGF (growth factor) promoting effect and promotes the growth of abalone and promotes immune function

The herbal medicine used in the present invention is widely used as a medicament since the medicinal effect is described in detail in the medicinal herb medicine.

Glucoside and saponin are the main ingredients of astragalus, and they are used as expectorants for bronchitis, and are effective for anemia, insomnia,

Bokbunja is a medicinal herb of wild raspberries belonging to Rosaceae. It has antioxidant, anticholesterol, liver and gastrointestinal function improvement. Its efficacy is mentioned in various documents such as Dongbibogam, In pharmacological analysis of medicine, it has been found that polyphenol is contained in a large amount in the fruit, anti-cancer effect, anti-aging, prevention of arteriosclerosis, prevention of thrombosis,

Cynanchum wilfordii is a perennial plant belonging to the family Polygonaceae, having a height of 1-3 m, and its roots are thick roots. The main ingredients of white seaweed are cinanotoxin, phytotoxotoxin, etc., and it is used as a tonic and anti-

Phlomis umbrosa is a 1 m high perennial herb belonging to the Lamiaceae family, with a height of 70-150 ㎝ and a height of about 5 root. Leaves are used for food, roots and rootstocks are used for medicinal purposes. It is known to have pharmacological effects to protect the liver and kidneys. It contains alkaloids, essential oils and vitamins.

Therefore, when the compound feed additive of the present invention manufactured as described above is mixed with the general compound feed at a proper ratio and fed to the abalone, realization of environmentally friendly abalone breeding will be performed to strengthen the immunity and to prevent and treat various diseases, And the growth rate can be improved, so that the mortality rate can be reduced and the feed efficiency can be increased.

Production of functional feed additives

1-1. Preparation of fermented sea mustard extract

Dried seaweed is finely pulverized and then fermented and fermented in a fermentation seaweed fermented at a low temperature under the action of a fermenting microorganism for 20 to 30 hours at a temperature of 20 ° C to 40 ° C using Bacillus subtilis (Bacillus subtilis) An extract was obtained.

1-2. Manufacture of plant complex extracts

IGF (growth factor) was synthesized by mixing raw extracts of native oriental medicine, bokbunja extract, white mint extract, early stage extract and extract of Saururus chinensis extract in a composition ratio (weight ratio, equivalent ratio) of 1: 1: A composite extract of a fermented plant of the present invention was prepared as a composition for promoting abalone growth which increased the production amount.

1-3. Preparation of Functional Feed Additives including Fermented Sea Mustard Extract and Fermented Plant Combined Extract

1-1. The fermented wakame extract of the process and the plant complex extract obtained from the 1-2. Process were mixed at a total weight ratio of 10: 1 to prepare an abalone feed additive containing the fermented wakame extract as an active ingredient.

Manufacture of abalone formula containing functional food additive

The abalone feed additive obtained in the above step 1 was added to the prepared feed mixture in an amount of 15 wt%: 85 wt% based on the total weight ratio, and then mixed with stirring to prepare an abalone mixed feed supplemented with the functional feed additive of the present invention

Test example:

The commercial off-the-blend diets supplemented with the functional feed additive of the present invention produced in Example 2 were prepared as feedstuffs for four weeks without any addition of the functional additive of the present invention, and the feed intake rate, growth rate, The feed efficiency of the present invention was investigated. The compound feed added with the present invention showed a higher survival rate and a higher growth rate than the feed without the functional additive of the present invention,

The feed efficiency was significantly higher than that of the control diet. Therefore, it was found that the abalone feed supplemented with the present invention realized remarkable intake and digestibility and improved survival rate.

As a result, the use of the present invention ultimately provides a high-quality feed additive for the whole body, thereby promoting the growth of abalone and reducing the loss of feed due to an increase in the feed intake rate, thereby improving the productivity by reducing the feed cost, By solving the marine pollution problem, it was found that the economical and environmental friendly form of healthy abalone could be made possible.

Therefore, the overturned feed additive of the present invention significantly reduces the stress of the abalone, and the appetite becomes strong, and the growth rate and the disease prevention rate are remarkably improved, so that it is necessary to grow abalone from abalone hatching to spore production, Nutrition, and the growth of abalone more than natural feed, and enables stable supply.

Therefore, if the diets of the present invention prepared as described above are fed to abalone, realization of environmentally-friendly abalone breeding can strengthen immunity, prevent diseases and prevent diseases, grow healthy without infecting diseases and improve the growth rate, The feed efficiency can be increased.

As a result, the use of the present invention ultimately provides a high-quality mixed feed for the whole body, thereby promoting the growth of abalone and reducing the loss of feed due to an increase in the feed intake rate, thereby improving productivity by reducing feed costs, It is possible to make the environmentally friendly style of healthy abalone possible.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. .

Claims (2)

An overturned feed additive comprising seaweeds,
The abalone feed additive containing the fermented seaweed extract as an active ingredient,
After immersion and washing, the seaweed subjected to low-temperature vacuum drying is finely pulverized and then the fermented microorganism bacillus subtilis (Bacillus subtilis) is used to perform the fermentation microorganism action for 20 to 30 hours at a temperature of 20 to 40 ° C. The fermented seaweed extracts were fermented at low temperature and fermented. The crude extracts of the herb extracts, the extracts of the bokbunja, the bamboo extracts, the extracts of the early stages and the fermented extracts of the Saururus chinensis extracts were added to the fermented seaweed extract at a total weight ratio of 10 : 1, which comprises the fermented marine leaf extract as an active ingredient, and an abalone feed additive The method according to claim 1,
Drying of the seaweed is carried out by low temperature vacuum drying or sterilization and drying using a far infrared ray drying method so that the cell tissue is activated and the taste and color are unchanged, and an abalone feed additive