KR20160120712A - Mineral nutrition composition for cultivating organic mineral sprout and cosmetic composition or functional food comprising thereof - Google Patents

Abstract

The present invention relates to a mineral nutrient solution composition for manufacturing organic mineral sprouts, and a cosmetic composition or a functional food composition comprising an extract of the organic mineral sprouts. According to the present invention, the cosmetic composition comprising a sprout extract exhibits anti-aging effects, effects in reducing wrinkles, or effects in whitening the skin, thereby being used as a raw material of a functional cosmetic product. According to the present invention, in the case of using a mineral nutrient solution for cultivating sprouts, a user can cultivate the sprouts having increased organic mineral ingredients, and can obtain an extract containing large amounts of effective ingredients in a short period of time by using an ultrasonic extraction method during the manufacture of the sprout extract.

Description

TECHNICAL FIELD The present invention relates to a mineral nutrient composition and a functional food containing a mineral nutrient composition and an organic mineral sprout extract for producing an organic mineral sprout,

The present invention relates to a cosmetic composition comprising a mineral nutrient solution composition and an organic mineral sprout extract for producing an organic mineral sprout, wherein the cosmetic composition containing the extract of a sprout according to the present invention exhibits anti-aging, wrinkle- When the mineral nutrient solution according to the present invention is used for culturing sprouts, it is possible to cultivate a sprout having an enhanced organic mineral component. By using an ultrasonic extraction method in the production of a sprout extract, Containing extract can be obtained.

The main trend of cosmetics in recent years is phytochemical cosmetic ingredients based on botanical raw materials. Plant sprouts are rich in vitamins and secondary metabolites. Plant secondary metabolites are produced by plants as a defense against disasters such as diseases, pathogens, and dryness, and include flavonoids, saponins, monoterpenes, phytosterols, and isothiocyanates. Studies by Johns Hopkins University have reported that the isothiocyanate content of broccoli sprouts is 20 to 50 times that of mature broccoli. lately Various sprout raw materials are being used as raw materials for cosmetics.

On the other hand, Cress sprouts ( Lepidium sativum ) Isothiocyanate components are abundant and are used as raw materials for cosmetics. Isothiocyanates are phytonutrients that detoxify and neutralize environmental pollutants. Cress sprout extracts have been reported to activate cell detoxification systems and increase resistance to toxic oxides. It is commonly known as the cruciferous cabbage, broccoli, cauliflower, kale, rape-seed, mustard, radishes, horse radish, It has been studied that water and garden cress contain isothiocyanate.

Various plant sprouts are utilized as raw materials for cosmetics, and it is known that a large garden cress sprout is blended with a strobus bark extract to exhibit a skin whitening effect (Korean Patent No. 1,083,028). Mustard buds are said to increase the fat layer. Sunflower buds also have the effect of promoting the energy of the skin of the skin. The sesame sprout has been shown to have antioxidant abilities to inhibit skin stress caused by reactive oxygen species (total polyphenol content and antioxidant activity of sprout extracts, .

Recently, it has been reported that a large number of new products for mineral cosmetics are shipped. In addition, the problem of the side effect of mineral cosmetics is also highlighted. Titanium dioxide and zinc oxide, for example, are skin-protective substances that block light. However, it has been reported that pure zinc oxide is not used and harmful substances such as bismuth oxychloride are harmful to the skin. In addition, mineral cosmetics may cause allergies or harm to skin, and nanomolecular powdered mineral cosmetics may be inhaled into the lungs during use. Liquid products are recommended, but parabens, phthalates, fragrances, and so on. Therefore, in order to solve the side effects of mineral cosmetics, development studies on organic mineral cosmetics have been attempted.

The present invention aims at providing a cosmetic composition containing a bud extract, and in particular, to provide a functional cosmetic composition exhibiting anti-aging, wrinkle-improving or whitening effect.

The present invention also provides an organic mineral nutrient solution for producing a bud plant in which various trace minerals are enhanced.

It is an object of the present invention to produce a cosmetic raw material containing an extract of a sprout grown with organic minerals and cultivated using the organic mineral nutrient solution, and to develop cosmetics using the same.

The present invention aims at supplying organic and organic raw materials to domestic and overseas companies by developing organic derived ingredients from domestic produce organic seeds, and also by cultivating mineral-reinforced sprout seeds There is an object of invention in establishing technology.

The present invention also provides a physical and extraction method of extracts of sprouts suitable for organic cosmetics certification standards without developing chemical, physical, chemical and biological processes suitable for organic farming by utilizing the herbal medicine extraction technology by the present inventor.

The present invention aims at solving the above problems by providing an anti-aging, wrinkle-improving or whitening cosmetic composition containing one or more bud extracts selected from the group consisting of a ginseng extract, a barley bud extract and a buckwheat sprout extract.

The present invention aims at solving the above problems by using a mineral nutrient solution containing deep sea water and a mineral component as a nutrient solution for sprout cultivation.

The present invention aims at solving the above problems by producing an extract of a bud using an ultrasonic extraction method.

The cosmetic composition containing the bud extract according to the present invention exhibits anti-aging, wrinkle-improving and whitening effects and can be usefully used as a functional cosmetic raw material. In addition, when the mineral nutrient solution according to the present invention is used for sprout cultivation, it is possible to harvest the sprout having high organic mineral content, so that the sprout having improved functionality can be provided and the farm profit can be improved. In addition, by using an ultrasonic extraction method in the production of a bud extract, an extract containing an increased amount of an oil component in a short period of time can be obtained.

Fig. 1 shows the process of germination.
Fig. 2 shows a process for cultivating buckwheat buds.
Fig. 3 shows a barley bud growing process.
4 is a graph showing the DPPH scavenging activity of buckwheat buds, wheat germ, barley shoot extract and ascorbic acid.
Fig. 5 shows the cytotoxicity test results of the extracts of buckwheat, bud, and barley buds (fibroblasts / dermis).
Fig. 6 shows the cytotoxicity test results of buckwheat buds, buds, and barley buds extracts (HaCat cells / epidermis).
FIG. 7 shows the results of cytotoxicity tests of buckwheat, wheat, barley bud extract (B16F1 cells - melanocytes).
Fig. 8 shows the measurement results of whitening effect of kojic acid, buckwheat, wheat, and barley bud extract.
9 is a graph showing the results of measuring the content of procollagen.
10 is a graph showing the results of TNF-alpha measurement.
11 is a photograph showing a metal for electrolysis and a metal and electrolytic solution to be electrolyzed.
12 is a photograph showing an electrolytic mineral solution (below) using the prepared mineral liquid (top) and a deep sea water concentrate as a solvent.

The present invention relates to an anti-aging, wrinkle-improving or whitening cosmetic composition containing one or more bud extracts selected from the group consisting of a ginseng extract, a barley bud extract and a buckwheat sprout extract.

In one embodiment of the present invention, the cosmetic composition may exhibit an anti-inflammatory effect.

In one embodiment of the present invention, the bud extract is an ultrasound-extracted extract, and may be extracted using an ultrasound merge extraction method. More specifically, it is possible to carry out the extraction from the ultrasound assisted extractor by placing the bud material in the nonwoven fabric. The extract obtained after the extraction can be concentrated in a powder concentrator, but is not limited thereto. Unlike the conventional extraction method, the ultrasonic merging extractor can extract high purity in a short time by using ultrasonic waves. For example, an extraction solvent having a weight of 2 to 10 times the weight of the sample is added to the sample and the solution is heated at a temperature of 40 to 95 ° C, specifically 50 to 85 ° C, more specifically 60 to 80 ° C, for 1 to 10 hours, Hour, more specifically 2 to 6 hours, more particularly 2 to 3 hours. The ultrasonic extractor used for extraction may be a condition of 10 to 80 kHz, specifically 20 to 70 kHz, more specifically 30 to 60 kHz.

In one embodiment of the present invention, the bud extract can be extracted with water, C _{1 -4} lower alcohol or a mixed solvent thereof, and more specifically, can be extracted using water.

The present invention relates to an anti-aging, wrinkle-improving or whitening food containing one or more bud extracts selected from the group consisting of a ginseng extract, a barley bud extract and a buckwheat sprout extract. The ingestion of the food containing the extract of the sprouts according to the present invention prevents aging, improves wrinkles and whitening effect, and can be utilized as so-called eating cosmetics.

In one embodiment of the present invention, the food may exhibit anti-inflammatory effects in addition to anti-aging, wrinkle-improving and whitening effects.

In one embodiment of the present invention, the bud extract can be extracted with water, C _{1 -4} lower alcohol or a mixed solvent thereof, and more specifically, can be extracted using water.

In another aspect according to the present invention, the present invention relates to a method of treating seawater, And at least nine minerals selected from the group consisting of Co, Cr, Cu, Fe, Mg, Mn, Sr, Zn, Li, V, Se and Sn.

In one aspect according to the present invention, the present invention relates to a method of treating a deep sea water; And at least ten kinds of minerals selected from the group consisting of Co, Cr, Cu, Fe, Mg, Mn, Sr, Zn, Li, V, Se and Sn. And more specifically 12 species.

In one embodiment of the present invention, each mineral comprises 1 to 20 mg of Fe and Zn, respectively, per liter of deep ocean water; 0.5 to 10 mg each of Cu, Sr and Li; 0.1 to 5 mg of each of Mn, Sn, Se, Cr, V and Co can be added. Specifically, Fe and Zn are each added in an amount of 5 to 15 mg; 2 to 8 mg each of Cu, Sr and Li; 0.5-2 mg of Se, Cr, V and Co, respectively, and more specifically 10 mg of Fe and Zn, respectively; 5 mg each of Cu, Sr and Li, and 1 mg of Mn, Sn, Se, Cr, V and Co, respectively.

In another aspect of the present invention, the present invention provides an anti-aging, wrinkle-improving or whitening cosmetic composition containing an extract of a bud, a barley bud or a buckwheat bud grown using a mineral nutrient solution composition.

In one aspect of the present invention, the cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, a lotion, a cream, an essence, an astringent, an emulsion, a gel, a lipstick, a spray, a shampoo, a rinse, a treatment, a body cleanser, But are not limited to, at least one selected from the group consisting of a foam, a cleansing cream, a cleansing water, a bath agent, a pack, a massage agent, a face powder, a compact, a foundation, a two-way cake and a makeup base.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tragacanth.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinates, fatty acid amides Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following Examples and Comparative Examples which are not intended to be construed as limiting the scope of the present invention. It does not mean anything.

Example  One. Mineral liquid  Preparation and dosage

Deep-sea deep-sea water (Waterbis) was used as a mineral liquid base. The concentrate of deep sea water has a magnesium content of 70,000 ppm, a potassium content of 20,000 ppm, and a sodium content of 20,000 ppm (see Table 1). The addition of Fe, Mn, Zn, Sr, Se, Sn, Cr, Co and V which are insufficient minerals to deep seawater concentrate of the ocean was added. The process of producing mineral liquid by electrolysis is as follows:

(1) By electrolysis Mineral liquid  Produce

A high concentration of mineral solution was prepared by ionizing the metals of Ca, Mg, Fe, Li, Mn, Zn, Sr, Se, Sn, Cr, Co, Ti and V in water by electrolysis. The electrolytic device for ionic minerals is designed to freely regulate the voltage with the sliders and to generate the direct current by using the rectifier. The capacitor is installed to store and use the current of 20A, Circuit. The anode wire used for electrolysis was connected to a wire made of titanium, then a platinum plate was installed on the cathode, and a metal desired to ionize the anode was grounded and immersed in water. Ionization of the metal is achieved by applying electricity, raising the voltage appropriately according to the metal, increasing the flow of current, and introducing a salt or organic acid into the electrolyte solution to help dissolve the metal ions. At this time, the ionic metal dissolved in the anode can be coated to adhere to the cathode so that the cathode is isolated through the reverse osmosis membrane to allow the electrons to pass through but the metal ions can not pass therethrough so that the concentration can be continuously increased by dissolving in water.

After the metal was ionized through this device, the concentration of the mineral was measured through the ICP equipment or various measuring instruments and then used for the preparation of the nutrient solution. Most of the positive ions are grounded to the anode of the electrode to ionize it, and in the case of anion materials such as sulfur or selenium, the cathode is melted. Generally, to make 10 ppm of mineral nutrient solution, concentrated mineral solution should be made to be more than 200ppm.

(2) Deep sea water  Electrolysis using solvent Mineral liquid  pharmacy

At this time, the whole mineral liquid can be produced more economically by producing minerals such as deep sea water which can be easily obtained from nature, and the concentrate of deep water has a magnesium content of 70,000 ppm, a potassium content of 20,000 ppm, and a sodium content of 20,000 ppm, and it contains other trace elements. Mineral liquid can be prepared by adding insufficient minerals to this concentrate. However, considering the precipitation of minerals, minerals such as calcium, Fe, Mn, Zn, Sr, Se, Sn, Cr, Co, Ti and V are mixed and used separately.

Deep sea water treated (Mineral water concentrated) Analysis item Test result Test Methods importance 1.301 Non-medium Ca (mg / L) 10 TITRATION Mg (mg / L) 74,757 TITRATION K (mg / L) 21,082 IC Na (mg / L) 20,924 IC Cl ^- (mg / L) 183,704 IC SO ₄ ^- (mg / L) 69,356 IC

The contents of this test are as follows. Mineral solution containing Fe, Mn, Zn, Cu, Sr, Se, Cr, Co, and V was electrolyzed for mineral strengthening in deep sea water. The minerals were supplied at a dose of 5 mg / L for Fe, Zn, 10 mg / L for Cu, Sr and Li, and 1 mg / L for Se, Cr, Co and V (see Table 2).

Mineral input element                 Mineral input (mg / L) Fe (mg / L) 10 Zn (mg / L) 10 Cu (mg / L) 5 Sr (mg / L) 5 Li (mg / L) 5 Se (mg / L) One Cr (mg / L) One V (mg / L) One Co (mg / L) One

<Organic cosmetics development target Domestic production sprout organic agricultural product type survey>

There are also a variety of certified agricultural products in Korea that are subject to organic cosmetics development. As a result of the inventor's research, 23 types of certified organic agricultural products have been developed as raw materials for organic cosmetic cosmetics.

Domestic sprout organic agricultural products division Number of species Plant Type Herbs, wild vegetables 6 Ginseng, mountain garlic, safflower, tiger riding, perilla,  Grain 8 Wheat, barley, buckwheat, beer barley, bouquet, barley, rye, corn Dumplings 6 Beans, alfalfa, clover, peas, mung beans, peanuts Leafy vegetables 3 Wasabi, kale, fresh cherry,

Experimental Example  1. Grow bud test

The wheat seeds were treated with a solution in which the groundwater-only control and the minerals prepared in Table 2 were enriched. Buds were called for 18 hours and given daily fluids. The wheat was covered with a lid of 1 cm thick on top of each other, and the solution was replenished daily so that the solution did not dry out. After a week, the buds were observed. After 2 weeks, the germs were sampled and the contents of various minerals were measured by ICP.

Experimental Example  2. Buckwheat bud growth test

Buckwheat (Commercial buckwheat, Fagopyrum esculentum Moench) was grown in the house in the same manner as the wheat. The buckwheat was covered with a lid on a 2 cm thick cloth, and the solution was replenished daily so that the solution did not dry out. After 2 weeks, the buckwheat was harvested.

Experimental Example  3. Extensive cultivation test

Barley was grown by hydroponics. It was produced in an environmentally controlled facility in the grasshopper production system. On the 10th day after barley sowing, it was harvested and used as a raw material for extracting cosmetics. The sprout was about 30 cm long.

Experimental Example  4. Mineral Analysis

Buckwheat, wheat and barley were grown as sprouts, and samples were collected and the amount of minerals contained in the crops was measured by ICP at Sangji University Natural Science Center. The experimental procedure for each crop is as follows.

(1) Summary of Pretreatment Method

The dried samples were pretreated according to US EPA Method 3050B (Sediment, Sludge, and Soil Acid Digestion).

① After drying, approximately 1.0 ~ 2.0 g of homogeneously mixed sample was weighed accurately, placed in a dissolution vessel, and then 10 mL of 1: 1 nitric acid (for measuring trace metals, kanto) was injected. The decomposition vessel was covered with a watch plate, transferred to a heating plate, refluxed at 95 ± 5 ° C for 10 to 15 minutes, and then allowed to cool.

② 5 mL of a nitric acid stock solution was added and refluxed for 30 minutes. If brown smoke occurs, this indicates the oxidation of the sample by HNO ₃ and this step (5 mL injection of HNO ₃ stock solution) is repeated until no brown smoke is released.

③ After the sample decomposition of step ② was completed, it was cooled, and 2 mL of distilled water and 3 mL of 30% H ₂ O ₂ (for measuring trace metals, Merck) were injected. The vessel was covered with a watch glass and the covered vessel was placed back onto the heating plate to start the heating and the hydrogen peroxide reaction. Care must be taken to avoid sample loss due to excessively violent bubbles. The bubbles were heated to cool and the vessel was allowed to cool.

④ 30% H ₂ O ₂ was injected in 1 mL increments continuously, keeping the warmth until the bubbles were minimized or the appearance of the normal sample remained unchanged. At this time, the injected 30% H ₂ O ₂ did not exceed 10 mL in total.

5. Continue the acid-hydrogen peroxide decomposition by heating until the volume is reduced to approximately 5 mL by covering the digestion vessel with a watch glass, or by heating at 95 ± 5 ° C for 2 hours without boiling. The cover was kept so that the solution did not evaporate altogether.

⑥ After cooling the sample, the microparticles and the sample solution in the solution were separated by using a funnel and a filter paper (Whatman, No. 40) in a 100-mL volumetric flask and used as a sample for measurement.

(2) Method of analyzing sample

The luminescence intensity of the sample for measurement was measured according to the inductively coupled plasma emission spectroscopy and the content (/ kg) of the analyte was calculated from the calibration curve prepared by correlating the concentration of the standard substance with the luminescence intensity per analysis item. The ICP-OES instrument used for the analysis was an inductively coupled plasma optical spectrometer (Vista-MPX, Varian) and Table 4 summarizes the instrumental analysis conditions. For samples with a concentration outside the linear range of the calibration curve, the sample was diluted with distilled water and diluted to a concentration within the linear range of the calibration curve. All sample treatments minimized contamination, and the distilled water used for sample dilution and washing was a Millipore Nanopure system.

Experimental Example  5. Mineral content of sprouts by mineral treatment

(1) Trace mineral concentration of buckwheat bud

The mineral concentration of the buckwheat sprouts according to the mineral nutrient solution treatment of Example 1 is shown in Table 5. As a result, Li, Mn, Sr and Zn were increased in the mineral nutrient solution of Example 1. The lithium content of each treatment was increased from 0.19 in the control to 4.59 mg / kg in the treatment group. It is known that the lithium content of the soil is about 100 ppm, and the lithium content of the fruit is usually reported as 14 to 70 ㎍ / g, and the lithium content of the pepper is as high as 194 to 318 ㎍ / g (Lovkova et al., 2007).

The effect of manganese and zinc treatment was observed in the control water sprout cultivation and microelements. Zinc is an important component in plant pollen formation. The Zn content of the pollen is 80 mg / kg (Cakmak, 2008). The zinc content of rice was reported to be 10 to 40 mg / kg, and wheat to 13 to 68 mg / kg (Rashid and Ryan, 2004).

The strontium (Sr) treatment had a high absorption effect in the mineral nutrient solution treated in Example 1, but was not effective in the groundwater control. Natural strontium is not a radioactive element, and organisms can not distinguish strontium from calcium and absorb strontium. In animals, it forms bones and teeth and replaces some of the calcium. The body contains about 320 mg of strontium, and the bone contains 36 to 140 ppm. Since the 1950s, the benefits of strontium for human health have been extensively studied, and strontium compounds have been used as dietary supplements (Krutilina et al., 1999).

Mineral concentration of sprouts by mineral treatment of buckwheat element strain Sprout (water application - control) Sprouts (mineral treatment) Concentration difference (bud-seed) Concentration difference (treatment-control) Fe (mg / kg) 61.2 70.7 72.5 9.5 1.8 V (mg / kg) 0.25 0.41 0.45 0.16 0.04 Cr (mg / kg) 0.85 1.01 1.12 0.16 0.11 Li (mg / kg) 0.15 0.19 4.59 0.04 4.4 Mn (mg / kg) 15.5 16.7 26.5 1.2 9.8 Co (mg / kg) 0.00 0.00 0.08 0 0.08 Cu (mg / kg) 6.52 7.78 9.98 1.26 2.2 Zn (mg / kg) 20.5 29.8 39.5 9.3 9.7 Se (mg / kg) 0 0 0.62 0 0.62 Sr (mg / kg) 0.35 0.43 5.80 0.08 5.37

(2) Mineral content of wheat according to mineral treatment

Table 6 shows the amount of minerals absorbed by the mineral nutrient solution treatment of Example 1 of the wheat germ. The contents of iron, lithium, strontium, manganese, selenium and strontium were greatly increased.

Mineral concentration of sprouts by mineral treatment of wheat element strain Sprouts (groundwater - control) Sprouts (mineral treatment) Concentration difference (bud-seed) Concentration difference (treatment-control) Fe (mg / kg) 52.2 67.7 73.6 15.5 5.9 V (mg / kg) 0.55 0.74 1.33 0.19 0.59 Cr (mg / kg) 0.52 0.61 1.09 0.09 0.48 Li (mg / kg) 0.42 0.45 5.20 0.03 4.75 Mn (mg / kg) 38.8 40.2 65.4 1.40 25.2 Co (mg / kg) 0.04 0.04 1.30 0 1.26 Cu (mg / kg) 4.70 4.87 7.55 0.17 2.68 Zn (mg / kg) 38.9 39.5 44.5 0.60 5.0 Se (mg / kg) 0.13 0.15 5.45 5.32 5.30 Sr (mg / kg) 1.55 1.59 12.46 0.04 10.8

(3) Mineral content of barley sprouts by mineral treatment

Iron, copper, manganese and zinc showed high content in barley bud. Barley buds contain these nutrients in a balanced manner. Table 7 shows the amount of minerals absorbed by the mineral nutrient solution treatment of barley bud of Example 1. Iron, lithium, strontium, manganese, selenium and strontium contents were greatly increased.

Mineral concentration of sprouts by mineral treatment of barley (barey) element strain Sprout (water application - control) Sprouts (mineral treatment) Concentration (sprout - seed) Concentration difference (treatment-control) Fe (mg / kg) 75.7 76.5 85.5 0.8 9.0 V (mg / kg) 0.45 0.65 1.39 0.20 0.74 Cr (mg / kg) 0.52 0.61 1.09 0.09 0.48 Li (mg / kg) 0.40 0.42 4.97 0.02 4.55 Mn (mg / kg) 18.9 20.2 45.1 1.40 24.9 Co (mg / kg) 0.04 0.04 1.23 0 1.19 Cu (mg / kg) 9.50 10.8 15.7 1.3 4.90 Zn (mg / kg) 19.5 52.8 64.7 33.3 11.9 Se (mg / kg) 0.14 0.16 4.58 0.02 4.42 Sr (mg / kg) 1.44 1.48 11.32 0.04 9.84

(4) Residual pesticide test results

Table 8 shows the results of the detection of residual pesticides in buds. As a result of analysis of 245 components such as EPN, residual pesticides were not detected in all samples.

Residual pesticide test results of buds sample sample water ingredient Residual pesticide concentration
(mg / kg) Remarks (analysis item) Buckwheat sprouts 2 - 0 EPN, etc. 245 component Barley bud 2 - 0 EPN, etc. 245 component Shine 2 0 0 EPN, etc. 245 component

Manufacturing example  1. Cosmetic material extraction process using sprouts

(1) Extraction, Powder Concentration Manufacturing Process

The sprout material was placed in a nonwoven fabric and the extraction was carried out in an ultrasound assisted extraction machine (Sonimed, Inc.). The extracted extract was put into a powder concentrator and concentrated. This ultrasonic merger and extraction device is a technology that enables the extraction of high purity in a short time even at low temperature by utilizing ultrasonic wave in the traditional hot water extraction method such as the traditional hot water extraction method.

(2) Extraction method

Buckwheat, wheat and barley sprouts were extracted at 70 ℃ for 2 ~ 3 hours using 5 times distilled water as the extraction solvent. Ultrasonic extractor (Sonimedi Co. 40 kHz, used for extraction) was used. Each of the obtained extracts was filtered with a standard, concentrated and then lyophilized, and the yields of the respective extracts were calculated.

Materials such as germinated brown rice were extracted by ultrasonic wave milling and ultrasonic treatment for 2 ~ 3 hours in an ultrasonic water bath. Ultrasonically extracted extracts were not extracted repeatedly, but after one extraction and filtration, they were used in the experiment. The extracts were stored in cold storage and used in experiments. The extraction yield of each extraction yield was calculated by the following equation (Woo and Lee, 2008).

Extraction yield (%) = (A x B / C) x 100

A: Soluble solids concentration (mg · mL ^-1 ), B: Amount of total extraction solvent (mL)

C: Amount of freeze-dried sample (mg)

(3) Extraction yield

The highest extractable amount of acid garlic shoots in ultrasonic extracts. As can be seen in Table 9, the extracts of wheat germ extract showed higher extraction yield than buckwheat sprouts. The high yield of ultrasonic extracts seems to be due to the synergistic action of mutual interaction with liquid by causing deaeration phenomenon which releases dissolved oxygen or bubbles out of the oven during ultrasonic irradiation. The reflux cooling extraction method is a method for stably extracting soluble materials of plants (Cho et al., 2005; Choi et al., 2005), but it has disadvantages such as destruction of useful components due to heat, . On the other hand, the ultrasonic extraction method is an extraction method which can extract the extracts at a low temperature to reduce the destruction of the useful components and expect a high extraction yield and physiological activity effect in a short time (Park et al., 2004; Kim et al., 2006).

Experimental Example  6. Skin Efficacy Test

(1) Antioxidative effect

1) Test method: Antioxidant activity measurement: DPPH method

500 uM DPPH (2.2-diphenyl-1-picryl hydrezyl) solution was dissolved in ethyl alcohol, filtered and used immediately. The prepared DPPH solution and the sample solution were mixed at a ratio of 1: 1 and mixed vigorously. The mixture was allowed to stand at room temperature for 20 minutes. After measuring the absorbance at 540 nm, the DPPH radical removal rate (%) was calculated using the following equation. At this time, 1 mM ascorbic acid was used as a positive control to remove radicals.

DPPH radical removal rate (scavenging,%) = (A - B) / A x 100

Where A is the absorbance of the test solution and B is the absorbance of the test solution.

2) Test result

Overall, the antioxidant potency was not as high as in the positive control group. In the case of wheat and barley shoots, high antioxidant activity was shown at high concentration.

(2) Cytotoxicity, cell growth experiment

1) Test method

Human Fibroblast, Keratinocyte, and Melanocyte were mixed in a 96-well flat-bottom microplate in an appropriate amount, and cultured for 24 hours to allow the cells to adhere to the bottom surface . After the cells adhered to the bottom surface, the culture solution was removed, and 100 μl of each concentration of the sample was added to each well and cultured in an incubator for 24 hours. After incubation, the effect on cell growth was measured by MTT (3- [4,5-dimethyl-thiazol-2-yl] -2,5-diphenyltetrazohum bromide) assay.

MTT assay was performed to evaluate cell growth and cytotoxicity of each natural product sample. 7 × 10 ³ fibroblasts were inoculated on a 96-well plate, cultured for 24 hours, and further cultured for 24 hours. The treated group was used as a control and compared with the results of the samples. After culturing for 24 hours after the extraction, MTT powder (3- [4,5-Dimethylthiazol2-yl] -2,5-diphenyltetrazolium bromide, Sigma, USA) was added to the DMEM medium with 0.33 g / L Was added to 100 μl / well of MTT solution and incubated at 37 ° C for 90 minutes. The reaction was then terminated and further reacted with 100 μl / well of DMSO (dimethyl sulfoxide) at room temperature for 30 minutes. After the reaction, the absorbance at 570 nm was measured using a microplate reader (Perkin Elmer, USA).

2) Test result

(1) Cytotoxicity and cell growth The results of the fibroblast (dermis) experiment are as shown in Fig.

(2) Cytotoxicity and cell growth The results of the HaCat cell (epidermal) experiment are as shown in FIG.

(3) Cytotoxicity and cell growth The results of the B16F1 cell (melanocyte-forming cell) experiment are as shown in FIG.

(3) Whitening test tyrosinase  Inhibition experiment)

1) Experimental method

The effectiveness of the whitening activity was determined by the mushroom tyrosinase inhibition assay (Mushroom tyrosinase inhibition assay). 20 μl of the sample solution was added to a mixed solution of 180 μl of distilled water, 200 μl of 0.1 M potassium dihydrogen phosphate and 200 μl of the substrate solution, and 20 μl of mushroom tyrosinase enzyme solution was added thereto. The mixture was shaken and incubated at 37 ° C. for 10 minutes Lt; / RTI &gt; The reaction solution was left in ice for 5 minutes and absorbance was measured at 490 nm (absorbance B).

Absorbance (absorbance A) was measured by using 20 μl of distilled water instead of the sample solution and using the same solution as the sample solution. A mixed solution of 380 μl of distilled water and 200 μl of 0.1 M potassium dihydrogen phosphate was added to 20 μl of the enzyme solution, and the solution was treated in the same manner as the sample solution to give a blank (control). At this time, 100 uM of kojic acid was used as a positive control to inhibit tyrosinase. The inhibition rate (%) of tyrosinase was calculated by the following equation.

Tyrosinase inhibition rate (%) = (A - B) / A x 100

1 mM ascorbic acid was used as a positive control to remove radicals.

2) Test result

① The synthesis of melanin in cells is very complex and controlled by various enzymes and substances. Among them, tyrosinase, a kind of polymerase that regulates the rate determining step, is the most important enzyme. The whitening functional cosmetic material prevents melanin pigment from depositing on the skin and functions to help skin whitening by suppressing the generation of spots and freckles. The result of this test showed whitening effect in barley buds.

Effect of inhibiting tyrosinase Kojic 80 Concentration (wt%) Buckwheat sprouts Shine Barley bud 0.09 1.43 29.57 7.15 0.03 -2.03 7.90 9.26 0.09 -3.54 -2.63 7.00

② The inhibitory effect of tyrosinase, which is important for the production of melanin, is lower than that of kojic acid, which is a control group.

(4) Whitening test (Cell-based assay)

1) Experimental method: Melanin analysis using B16F1

In the case of the melanin assay, a concentration of 1.5 x 10 &lt; ⁵ &gt; B16F1 melanoma cells were inoculated on a 6-well plate and incubated for 24 hours. Melanocyte stimulating hormone (5 nM) and melanocyte stimulating hormone (Melanocyte stimulating hormone) were used to induce melanin synthesis. And 10% FBS, 1% PS) to inhibit melanin synthesis for 72 hours. In the case of α-MSH treatment, a group treated with 800, 1,600 μM kojic acid, which is known to have no effect on treatment and melanin synthesis inhibition, was compared with the results of the test.

In the case of melanin analysis, the extract was treated with B16F1 melanoma and cultured for 72 hours, and the secreted melanin secreted into the medium was measured. After 72 hours of culture, the medium was recovered and centrifuged at 14,000 rpm for 10 minutes. The supernatant was transferred to a 96-well plate and absorbance was measured at 405 nm using a microplate reader (Perkin Elmer, USA).

2) Experimental results

In the case of wheat germ, barley shoots have an excellent effect of inhibiting melanin production caused by melanin synthesizing hormone in melanin-forming cells.

(5) Wrinkle improvement effect procollagen  assay)

1) Experimental method: Photoaging  Inhibitory effect Experiment: Procollagen ( procollagen ) Content measurement

The main cause of wrinkles is the decomposition of collagen in the dermis by UV exposure.

In order to measure the photoaging inhibition effect of the present study, a procollagen assay was performed to measure the amount of collagen newly produced in the fibroblasts under UV irradiation conditions.

Fibroblasts, one of the cell lines involved in collagen production, were cultured in Dulbecco's modified essential medium (DMEM, Sigma) containing 1% Antibiotic-Antimycotic (AA, Welgene, Korea) and 10% fetal bovine serum (FBS, Welgene, Korea) Welgene, Korea) at 37 ° C in a 5% CO ₂ incubator. 2 × 10 ⁵ fibroblasts were inoculated on a 6-well plate and cultured for 24 hours. The medium was removed and starved for 24 hours in serum-free medium. After 24 hours, fetal bovine serum was removed, washed with PBS, and irradiated at 12.5 mJ at a wavelength of 312 nm. Thereafter, the sample was treated with phenol red-free DMEM medium for each concentration and further cultured for 48 hours. Ascorbic acid (50 uM) was used as a positive control, the UV (+) group as a negative control, and the roughened group as a control group.

After 48 hours of cultivation, the medium and cell lysate were collected, respectively. RIPA buffer containing protease inhibitor was added to each well and the cell lysate was collected on a cell scraper on ice. After centrifugation at 14,000 rpm for 10 minutes, the supernatant was recovered and the amount of protein was corrected using a Bradford assay.

The amount of collagen was measured using a Procollagen Type-I C-Peptide (PIP) EIA kit (Takara Bio Inc. MK101). 100 μl of Antibody-POD conjugate solution was added to each well and 20 μl of sample or standard was added. The mixture was mixed well and left at 37 for 3 hours. After the reaction was completed, the contents were removed and washed 4 times with 400 μl of PBS. After removal, 100 μl of substrate solution was added, reacted at room temperature for 15 minutes, and 100 μl of stop solution was added thereto and gently mixed. After measuring the absorbance at 450 nm, a standard concentration curve was prepared and the amount of collagen was calculated.

2) Experimental results

Wheat, barley and buckwheat sprouts were found to have the effect of inhibiting collagen degradation by ultraviolet rays.

(6) Anti-inflammatory effect TNF -alpha assay)

1) Experimental method: TNF -alpha assay

TNF-alpha is a pro-inflammatory cytokine that is used as an indicator of inflammatory response inhibition. 1 × 10 ⁵ HacaT cells were inoculated on a 12-well plate and incubated for 24 hours. After incubation for 24 hours, the samples were incubated with UV irradiation and incubated for 24 h in order to induce TNF-alpha. When the UV treatment was not performed, the results of the samples were compared using a group treated with UV and treated with 1 uM / ml of dexamethasone as a control.

In the case of TNF-alpha assay, HacaT cells were irradiated with 12.5 mJ at 312 nm, and the TNF-alpha secreted by the medium was measured after 24 hours of treatment with the extract. The recovered medium was measured for absorbance at 450 nm using a TNF-alpha kit (Invitrogen # KHC3012) using a microplate reader (Perkin Elmer, USA).

2) Experimental results

Barley sprouts showed a stable anti-inflammatory effect. The buckwheat sprouts also showed excellent anti - inflammatory effect. In the case of wheat germ, the anti - inflammatory effect showed a good anti - inflammatory effect at low concentration, but the anti - inflammatory effect was decreased as the concentration increased.

(7) Skin Efficacy Test Results

Overall, the antioxidant activity of the bud extract was not higher than that of the positive control, but the antioxidant activity of the buds and barley buds was high at high concentrations. Wheat, barley and buckwheat sprouts were found to have the effect of inhibiting collagen degradation by ultraviolet rays. In the case of wheat germ, the melanin-forming cells were found to have an excellent inhibitory effect on the melanin synthesis induced by the melanin synthesizing hormone. Skin efficacy test results are summarized as follows. The wrinkle - improving effect was excellent in wheat, barley buds, whitening effect, barley buds, anti - inflammatory effect, barley buds and buckwheat buds.

<Preparation of cosmetics>

1. Extract preparation

Extracts were prepared to maximize the content of phytochemicals.

2. Development of phyto-minerals reinforced cosmetic materials

Vitamin A palmitate 1-2 g Zinc sulfate 20 to 60 g alpha -tocopherol acetate 5-15 g Lyophilized sprout powder 10-25 g Thyme oil 5-12 g Cream base Total amount is 1000.00 g

3. Manufacture of sprout cream

Vitamin A palmitate 0.825 g Zinc sulfate 22.0 g alpha -tocopherol acetate 5.0 g Sodium selenate 2.5 g Lyophilized sprout powder 7.5 g Thyme oil 4.0 g Cream base Total amount is 500.0 g

4. Manufacture of soap

330 mL of water and 175 g of NaOH were mixed to dissolve completely, and then 10 mg of the sprout extract was mixed with little addition for about 30 minutes. The mixture was allowed to dry in a shady, windy environment until dry.

5. Manufacture of bath agent

10 mg of the sprout extract was allowed to reach about 60 ° C, and then the salt solution was added to make a saturated solution, and the water was evaporated. The solution was cooled at room temperature and freeze-dried by rapid freezing. The lyophilized solid was powdered to prepare a bath agent.

6. Cleansing lotion manufacturing

Sprout extract 0.001 v / v% Sodium ethylenediaminetetraacetate 0.02 Paraoxybenzoic acid methyl 0.2 glycerin 4 Sodium Hyruronic Acid 2 Propylene glycol 3 Carbomer 5 Cetearyl alcohol 0.7 Glyceryl stearate 0.5 Sheer butter One P-hydroxybenzoic acid profile 0.1 Macadamia nut oil One Sesquioleic acid 0.5 Glyceryl stearate One Polyoxyethylene sorbitan monooleate 2 Polydensen 5 Mineral oil 20 Dimethicone 5 Stearyl dimethicone 2 Triethanolamine 0.05 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare the cleansing lotion according to the conventional method in the field of cosmetic production.

7. Skin production

Sprout extract 0.001 v / v% Paraoxybenzoic acid methyl 0.2 Sodium ethylenediaminetetraacetate 0.02 1,3-butylene glycol 0.02 Allantoin 3 Sodium hyaluronic acid seed 5 Carbomer 0.1 Cetostearyl alcohol 0.7 P-hydroxybenzoic acid profile 0.1 Sorbitan olivate 1.5 Soy lecithin 0.2 Dimethicone 0.2 Cetyl octanoate 0.2 Sheer butter 0.2 Sodium placrylate 3 Triethanolamine 0.1 Imidazolidinyl urea 0.3 Spices Suitable amount Pigment Suitable amount Purified water Balance

These ingredients were used to make the skin according to the conventional method in the field of cosmetics for making a skin.

8. Serum manufacturing

Sprout extract 0.001 v / v% Jojoba 5 Black Sesame 2 Sweet almond 3 This malting wax One Vitamin E One glycerin 2 Hyaluronic acid One Marine Elastin One Purified water Balance

The above ingredients were used to prepare according to the conventional method in the field of cosmetic production for the production of serum.

9. Manufacture of lotion

Sprout extract 0.001 v / v% Paraoxybenzoic acid methyl 0.2 Sodium ethylenediaminetetraacetate 0.02 1,3-butylene glycol 0.02 Allantoin 3 Sodium hyaluronic acid seed 5 Carbomer 0.1 Cetostearyl alcohol 0.7 P-hydroxybenzoic acid profile 0.1 Sorbitan olivate 1.5 Soy lecithin 0.2 Dimethicone 0.2 Cetyl octanoate 0.2 Sheer butter 0.2 Sodium placrylate 3 Triethanolamine 0.1 Imidazolidinyl urea 0.3 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare according to conventional methods in the field of cosmetics for the manufacture of lotions.

10. Essence manufacturing

Sprout extract 0.001 v / v% Allantoin 0.05 Sodium ethylenediaminetetraacetate 0.02 Triethanolamine 0.2 Sodium Hyaluronic Acid 7 Imidazolidinyl urea 0.15 Sodium polyacrylate 0.4 Carbomer 0.2 ethanol 3 Polyoxyethylene sorbitan monostearate 0.2 Paraoxybenzoic acid methyl 0.2 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare according to a conventional method in the field of cosmetic preparation for essence production.

11. Cream production

Sprout extract 0.001 v / v% Sodium ethylenediaminetetraacetate 0.02 Allantoin 0.1 glycerin 5 Paraoxybenzoic acid methyl 0.2 Sodium Hyaluronic Acid 6 Carbomer 0.1 Cetostearyl alcohol 1.7 Polydensen 2 Squalane 2 P-hydroxybenzoic acid profile 0.1 Butylene glycol dicaprylate 3 Cetyl octanoate 5 Microcrystalline lead 0.1 Triethylpentanediol 0.1 Sheer butter 0.2 Sorbitan olivate 0.3 Cyclomethicone 0.3 Stearyl dimethicone 0.5 Imidazolidinyl urea 0.15 Spices Suitable amount Pigment Suitable amount Purified water Suitable amount

These ingredients were used to prepare the cream according to the conventional method in the field of cosmetic production.

12. Pack manufacture

Sprout extract 0.001 v / v% Sodium ethylenediaminetetraacetate 0.02 Betaine 3 Glyceryl polymethacrylate 2 Allantoin 0.1 Sodium Hyruronic Acid 2 glycerin 3 Dipropylene glycol 5 Paraoxybenzoic acid methyl 0.2 Polyvinyl alcohol 10 Polyoxyethylene sorbitan monooleate 0.9 Sesquioleic acid 0.3 Jojoba ester 2 Cetearyl alcohol 1.5 Petrolatum 0.5 Spices Suitable amount Pigment Suitable amount Purified water Suitable amount

The ingredients were prepared according to the conventional method in the field of cosmetic production for pack manufacture.

13. Massage cream manufacturing

Sprout extract 0.001 v / v% Purified water Suitable amount glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.5 Squalane 2.5 Wax 2.1 Tocopheryl acetate 0.1 Cava Paul 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount

The ingredients were used to make the usual methods in the field of cosmetic manufacturing for the production of massage cream.

14. Manufacture of makeup base

Sprout extract 0.001 v / v% Purified water Suitable amount Coated silica One Silica 10 Titanium dioxide 8 Zinc oxide 3 Pigment One Plate powder Balance

These ingredients were used to make the makeup base according to the conventional method in the field of cosmetic production.

15. Powder fact manufacturing

Sprout extract 0.001 v / v% Purified water Suitable amount Mica 15 Titanium dioxide 7 Silicone oil 3 Ester oil 3 Pigment Suitable amount Spices Suitable amount Talc Balance

The powder facts were prepared according to a conventional method in the field of cosmetic production for the powder fact using the above-mentioned components.

16. Two way cake production

Sprout extract 0.001 v / v% Purified water Suitable amount Mica 15 Titanium dioxide 12 Silicone oil 3 Ester oil 5 Pigment Suitable amount Spices Suitable amount Talc Balance

The ingredients were used to make the usual methods in the field of cosmetics for the manufacture of two-way cakes.

Claims (1)

Deep sea water; And 1 to 20 mg of Fe and Zn, respectively, per liter of the deep sea water; 0.5 to 10 mg each of Cu, Sr and Li; And 0.1 to 5 mg of Mn, Sn, Se, Cr, V and Co, respectively, are added to the cosmetic composition for improving wrinkles, wherein the composition comprises a barley bud extract grown using a mineral nutrient solution composition.

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