KR20160096548A - Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition comprising the same - Google Patents

Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition comprising the same Download PDF

Info

Publication number
KR20160096548A
KR20160096548A KR1020160012240A KR20160012240A KR20160096548A KR 20160096548 A KR20160096548 A KR 20160096548A KR 1020160012240 A KR1020160012240 A KR 1020160012240A KR 20160012240 A KR20160012240 A KR 20160012240A KR 20160096548 A KR20160096548 A KR 20160096548A
Authority
KR
South Korea
Prior art keywords
parts
weight
skin
aging
crickets
Prior art date
Application number
KR1020160012240A
Other languages
Korean (ko)
Other versions
KR101809451B1 (en
Inventor
이삼구
Original Assignee
이삼구
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56854511&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR20160096548(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 이삼구 filed Critical 이삼구
Priority to PCT/KR2016/001161 priority Critical patent/WO2016126096A1/en
Publication of KR20160096548A publication Critical patent/KR20160096548A/en
Application granted granted Critical
Publication of KR101809451B1 publication Critical patent/KR101809451B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0212Face masks
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/46Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin

Abstract

The present invention relates to a cricket having enhanced antioxidant, anti-aging, and skin beautification improving, and fatigue relieving effects, and to a cosmetic composition containing the same. The cricket is raised by being fed with a fodder produced to be able to accumulate proteins and nutrients which are effective in ensuring anti-oxidation and anti-aging and relieving fatigue in the cricket at high capacity. The cricket having enhanced antioxidant, anti-aging, and skin beautification improving, and fatigue relieving effects according to the present invention may be raised by being fed with a fodder including 7-8 parts by weight of tomatoes, 7-8 parts by weight of spinach, 7-8 parts by weight of carrots, 7-8 parts by weight of lettuces, 7-8 parts by weight of bean sprouts, 7-8 parts by weight of Chinese cabbages, 7-8 parts by weight of spring onions, 7-8 parts by weight of wormwood, 7-8 parts by weight of chives, 7-8 parts by weight of eggplants, 7-8 parts by weight of fast-fermented beans, 7-8 parts by weight of cabbages, 7-8 parts by weight of Artemisia capillaris Thunberg, and 7-8 parts by weight of kales with respect to 100 parts by weight of rice bran.

Description

(Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition containing the same)

The present invention relates to a cricket improved in antioxidation, anti-aging and skin-beauty improving effect and a cosmetic composition containing the same, and more particularly to a cricket which is produced so that a protein effectively acting on skin improvement can be accumulated in a large amount in the body of crickets The present invention relates to a cricket and a cosmetic composition containing the cricket, wherein the feed is fed to a cricket to enhance antioxidation, anti-aging and skin-beauty improving effects.

Skin is a part of the body that is directly exposed to the external environment. Exposure to excessive ultraviolet rays or pollutants causes skin irritation such as erythema, edema, itching, and inflammatory reaction. Such skin troubles are not only cosmetic problems, but also substances which are produced in the inflammatory reaction cause collateral pigmentation, promote skin collapse of elastic fibers, and increase skin wrinkles. Such skin damage is known to be associated with free radicals, and chemically free radicals are independently present species with one or more unpaired electrons.

The theory of free radicals was presented by D. Harman in the mid-1950s, and has recently gained attention in the academia and related industries. Free radicals cause various problems such as wrinkle formation, skin cancer, cell killing, rheumatoid arthritis, atopic dermatitis, acne and the like because it destroys cells or cuts or cross-links connective tissue of skin dermal layer.

Superoxide dismutase (SOD), catalase, vitamin E, vitamin C and ubiquinol exist in the human body. However, antioxidant system (antioxidant system) And the free radicals gradually increase.

The free radicals produced by excessive UV exposure can break collagen (collagen), elastin, hyaluronic acid, which are connective tissues of the dermis, and cause settlement phenomena (wrinkles) in certain areas of the skin. It can cause the destruction of cells by oxidizing the lipid part and cause diseases such as dermatitis, acne or skin cancer. In addition, radicals are involved in the melanin formation process, which can cause stains, freckles and wrinkles.

In addition, free radicals are known to stimulate pigment cells present in the skin to accelerate the spontaneous oxidation reaction (dopaquinone → melanin) during the production of melanin pigment to induce spots and freckles to darken the skin. Depending on its extent and location, it may also cause severe inflammation and impaired skin immunity.

Korean Patent Registration No. 10-1315325 discloses a conventional art having antioxidative effect which is currently used to solve the above problems. This is disclosed in Korean Patent Publication No. 10-1315325, which discloses an anti-oxidant composition for skin wrinkle improvement and skin whitening external application Wherein the composition for external application for skin contains two or more antioxidant components selected from the group consisting of Lipoic Acid, Tocopherol, Coenzyme Q10, Selenium and Vitamin C, To a composition for improving skin wrinkles and skin whitening comprising an antioxidant component having an excellent skin wrinkle improvement effect and skin whitening effect by eliminating free radicals or enhancing the activity of a skin-protecting factor associated with antioxidation .

The above-described prior art skin external composition composition has a free radical scavenging effect and has been used as a method for preventing wrinkles and other skin diseases by incorporating it into a cosmetic or a medicinal product. In addition to being expensive, There is a problem that it is difficult to obtain an effect.

Conventionally, ascorbic acid, alpha -tocopherol, or SOD has been used as a free radical scavenging functional material in cosmetics or pharmaceuticals to prevent wrinkles and other skin diseases. However, they are not only expensive but also have chemical stability It is difficult to expect a practical effect.

Therefore, in order to reduce skin irritation and inflammation caused by various stress sources, and to reduce adverse effects caused by the use of cosmetics, it is urgently required to develop a substance having antioxidant and skin irritation mitigating effect. In addition, natural extracts And the development of a cosmetic composition containing the extract has been required.

On the other hand, crickets are insects belonging to Gryllidae, which are common in our living environment. Although it is known as an emotional insect, it is a pest that eats fruit trees and various seedlings, but it is effective when it is used in a child's game and it is also used as a fever. It is known that adult humans are used for diarrhea and dysentery in Ahi Mai and Oita in Japan. In addition, in the case of typhoid fever, the juice is squeezed by pressing adult fishes. However, it has not been commercialized in general, and crickets are currently being sold in Zoomed in the US as reptiles and fish feeds.

In the present invention, it has been found that when crickets are fed with feeds prepared by blending specific materials, the content of specific nutrients (amino acids, etc.) increases in the crickets and that crickets have an excellent effect in preventing antioxidation and skin aging And a cricket and a cosmetic composition having antioxidative and anti-aging effect using the cricket.

Patent Registration No. 10-2014-0146415 Patent Registration No. 10-2003-0085837

Disclosure of the Invention The present invention has been conceived in order to solve the above-mentioned problems, and it is an object of the present invention to provide a method for manufacturing a cosmetic composition by using crickets to breed crickets so as to increase the content of active ingredients involved in antioxidant and anti- The present invention is to provide a cricket and a cosmetic composition containing the cricket which are improved in antioxidation, anti-aging, and skin-beauty improving effects that significantly improve the antioxidant, anti-aging and skin-

The cricket improved in antioxidation, anti-aging and skin-beauty improving effect according to the present invention is prepared by adding 7 to 8 parts by weight of tomatoes, 7 to 8 parts by weight of tomatoes, 7 to 8 parts by weight of carrot, 7 to 8 parts by weight of lettuce, 7 to 8 parts by weight of bean sprouts, 7 to 8 parts by weight of Chinese cabbage, 7 to 8 parts by weight of corn, 7 to 8 parts by weight of wormwood, 7 to 8 parts by weight of leek, 7 to 8 parts by weight of branches, 7 to 8 parts by weight of chungkukjang , 7 to 8 parts by weight of cabbage, 7 to 8 parts by weight of horse mugwort, and 7 to 8 parts by weight of kale were fed to cricket three times a day, and 0.005 g to 0.02 g was fed to each cricket per feeding, 0.75 to 0.85% of lysine, 3.80 to 4.20% of lysine, and a cricket with an improved antioxidant, anti-aging and skin-beauty improving effect, .

It is confirmed that the crickets improved in antioxidation, anti-aging and skin-beauty improving effect according to the present invention can be used as an environmentally-friendly cosmetic composition.

The cricket improved in antioxidation, anti-aging and skin-care improving effect according to the present invention can be used as a raw material of rice bran, tomato, spinach, carrot, lettuce, bean sprouts, Chinese cabbage, wormwood, leek, eggplant, chonggukjang, cabbage, So that the contents of methionine, lysine, vitamin B, C, E, lycopene, beta carotene, alpha carotene, saponin, tannin, polyphenol and omega 3 which are involved in antioxidant and anti-aging action are increased By raising crickets, it is possible to remarkably improve the skin-beauty-improving effect as compared with general crickets.

Thus, the cosmetic composition containing the cricket enhanced in antioxidation, anti-aging and skin-beauty improving effect has a high antioxidative activity with excellent free radical scavenging activity, and is excellent in cell protection and prolonged cell life, There is an effect of remarkably improving the skin-beauty-improving effect because there is an excellent effect for maintenance or wrinkle improvement.

The above cricket is a high nutrient material which can be mass-proliferated, which is an advantage of insect rearing, and has little side effects on the human body, unlike conventional cosmetics, which are difficult to expect a substantial effect due to high price and poor chemical stability when formulated. It is a natural extract that is safe enough to attract attention as a food resource, so it is easy to develop. In addition, it is possible to increase the income of farmers who are primary industry by recycling various vegetables used for insect breeding and feed, and it is possible to develop various products using the cricket as mentioned above, which is a useful resource expected to have high marketability in the future.

In general, fat-soluble vitamins such as sodium PCA, retinoid, squalane, vitamin A and vitamin B, which are highly permeable moisturizing agents, are used in basic cosmetics and pack components, It can be provided as a more natural and environmentally friendly cosmetic composition containing a large amount of the above nutrients while being utilized as a food.

Fig. 1 is a schematic view showing a test method for the effect of the cricket and the cosmetic composition containing the same, in which the antioxidative, antioxidative and skin-
Fig. 2 is a graph showing changes in the amount of vitamin in crickets raised by the present invention in%
Fig. 3 is a graph showing the amount of change of minerals in crickets raised by the present invention in%
FIG. 4 is a graph showing the amount of change in amino acid content of crickets raised by the present invention in%
Fig. 5 is a graph showing changes in moisture content measured in Test Example 2
Fig. 6 is a graph showing changes in oil content measured in Test Example 2
FIG. 7 is a graph showing the results of measurement of changes in skin condition due to the degree of wrinkles in Test Example 2

Advantages and features of embodiments of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below with reference to the accompanying drawings. However, the present invention is not limited to the embodiments and test examples described below, but may be implemented in various other forms, and these embodiments are not intended to limit the scope of the present invention, It is intended that the scope of the invention be defined by the claims and the equivalents thereof. Like reference numerals refer to like elements throughout the specification.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions in the embodiments of the present invention, which may vary depending on the intention of the user, the intention or the custom of the operator. Therefore, the definition should be based on the contents throughout this specification.

Hereinafter, embodiments of the present invention will be described in detail.

The cricket improved in antioxidation, anti-aging and skin-beauty improving effect according to the present invention is prepared by adding 7 to 8 parts by weight of tomatoes, 7 to 8 parts by weight of tomatoes, 7 to 8 parts by weight of carrot, 7 to 8 parts by weight of lettuce, 7 to 8 parts by weight of bean sprouts, 7 to 8 parts by weight of Chinese cabbage, 7 to 8 parts by weight of corn, 7 to 8 parts by weight of wormwood, 7 to 8 parts by weight of leek, 7 to 8 parts by weight of branches, 7 to 8 parts by weight of chungkukjang , 7 to 8 parts by weight of cabbage, 7 to 8 parts by weight of horse mackerel, and 7 to 8 parts by weight of kale. That is, the feed is to supply tomatoes, spinach, carrots, lettuce, bean sprouts, cabbage, parsley, wormwood, leek, eggplant, chungkukjang, cabbage, eggplant and kale as raw materials with rice bran as a main material and having a specific substrate. At this time, the main material and the sub material may be mixed at a weight ratio of 1: 0.98 to 1: 1.12. If the weight ratio of the sub material is more than 1.12, each material constituting the sub material having a specific substrate is strong, If it is less than 0.98, there is a problem that the efficacy of the material having a specific substrate may be lowered.

Rice bran is a pulverized mixture of perilla, seed and hornblende which occurs when brown rice is harvested. It is mainly used as a livestock feed as a by-product in a farm. Generally, about 8% of rice bran can be obtained in brown rice, and rice bran contains protein, fat, vitamin B group, and inorganic matter.

The standard composition ratio of rice bran is 13.5% for water, 13.2% for protein, 18.3% for fat, 38.3% for carbohydrate, 7.8% for fiber, 8.9% for mineral, vitamin A, vitamin B1, vitamin B6, vitamin E, iron, phosphorus, Are very rich in nutrients. Therefore, dietary nutrients needed for cricket growth can be supplied in a variety of ways by feeding feeds containing rice bran as the main ingredient.

In addition, it is economically effective to recycle by-products of farm households by feeding cricket, and it is also effective to prevent the import of livestock or insect feed.

The rice bran may be included in an amount of 45 to 55 parts by weight based on 100 parts by weight of the total feed.

Tomatoes are one of the most notable foods that are attracting attention as health foods because lycopene is the most important reason. Lycopene, which makes tomato red, is a powerful antioxidant that has the effect of preventing aging of cells by releasing active oxygen, which is the cause of aging. It also blocks ultraviolet rays and prevents skin aging.

Tomato contains a lot of antioxidant substances such as lycopene as well as beta carotene. In particular, red tomatoes contain a lot of lycopene, and red tomatoes usually contain 7-12mg of lycopene.

Therefore, not only lycopene excellent for skin aging, but also rich in vitamin C, antioxidant effect is excellent, so it is best to prevent the aging of the diet containing crickets to feed crickets to feed a certain amount of antioxidants and vitamin C containing a lot of functional crickets Can be produced.

The tomato can be heated. When heat is applied, lycopene is extracted from the cell wall of the tomato to increase the absorption rate of the cricket.

The tomato may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Spinach is an important health food because it contains iron 33mg, vitamin A 2,600IU, B1 0.12mg, B2 0.03mg, C 100mg, vitamin E, K, and folic acid in 100g of spinach. Spinach, which produces nitric oxide, contains a large amount of beta-carotene, which is an antioxidant that is effective in regenerating skin and preventing aging.

The feed containing spinach is fed to crickets to contain a large amount of beta carotene, which can produce crickets with enhanced antioxidant and anti-aging effects.

The spinach may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Carrots are rich in vitamin A, and vitamin A promotes the production of collagen, one of the essential components of the skin. Therefore, it is possible to prevent the aging of the skin because the collagen production is increased and the rough skin is quickly restored and the elasticity is increased to prevent wrinkles.

In addition, natural carotenoid components such as excreted carotene and alpha carotene are abundantly contained. Beta-carotene, which is converted into vitamin A in the body, is an antioxidant that removes active oxygen that damages cell membranes and genes, promotes the expression of cancer-suppressing genes, and inhibits phospholipid metabolism involved in cell mutation.

Feeding the feed containing carrots to crickets can produce functional crickets that have higher antioxidant and anti-aging effects than the crickets fed with common feed of vitamin A and beta-carotene.

The carrot may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Since lettuce consists of more than 95% of water, it is effective to moisturize the skin when ingested. It also contains vitamins and minerals, so it is effective for skin whitening and elasticity enhancement. It also contains vitamin A, so it has anti-aging effect. Therefore, feeds including lettuce can be fed to crickets to produce functional crickets with anti-aging effects.

The lettuce may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Soybean sprouts are rich in vitamin C and minerals such as methionine and saponin, and are effective in skin care, such as regenerating upper skin by ultraviolet rays. In particular, methionine, which is an essential amino acid, can be cricket containing proteins that are synthesized as proteins and effective for skin regeneration when crickets are ingested.

In addition, methionine, along with vitamin C antioxidant, anti-aging action, skin beauty and liver function improvement is effective for fatigue recovery. Therefore, feeds containing bean sprouts can be fed to crickets to produce functional crickets with anti-aging effects.

The bean sprouts may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Chinese cabbage contains 33 IU of vitamin A, 100 IU of carotene, 0.05 mg of vitamin B1, 0.05 mg of vitamin B2, 0.5 mg of nicotinic acid, and 40 mg of vitamin C in 100 g. There is no vitamin A in the white part which is soft white and it is contained in the blue part, so the blue part of the cabbage is used as the above feed.

The Chinese cabbage may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

It contains calcium, phosphorus, iron, sulfur, magnesium, vitamin C, etc. in large quantities, especially allyl sulfide. Allyl sulfide combines with vitamin B1 to form allythiamine, which stimulates the metabolism of the body. Allyl sulphide is present in many slippery areas of the waves and is effective for sedation because it has sedative effect.

Vitamin C, which is contained in large amounts in the white strips and green leaves of the wings, is a powerful antioxidant and has an anti-aging effect.

In addition, crickets contain 5 times more magnesium than the same amount of beef, but in order to increase the content of crickets, crickets have been supplemented with insufficient nutrients. However, when the crickets are fed, it is possible to preserve the population because the content of magnesium can be increased without feeding allergens. Therefore, the content of magnesium is increased, and crickets that are effective for skin care and aging prevention can be produced.

The wave may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Mugwort contains antioxidant function that suppresses the production of lipid peroxide because it contains tannin component, and it has an effect of preventing aging of cells. In addition, mugwort also contains beta carotene, which has excellent antioxidant effect. When mugwort is included in feed as described above, tinctin and beta-carotene are taken into the diet to produce functional crickets that are effective in skin care and anti-aging.

The mugwort may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Leek is rich in beta-carotene, so it has the effect of preventing the generation of active oxygen, known as the cause of aging, in the body. In addition, leek is a vegetable that warms the body and contains allergen, vitamin E, selenium, dietary fiber, etc. Selenium is a component of enzymes that remove active oxygen poison. Alen is a sulfur compound, It has the effect of decrypting.

Therefore, the feed containing the antioxidant substances beta-carotene, lean and selenium-containing leeks can be fed to crickets to produce antioxidant and anti-aging functions, thereby producing a functional cricket having an anti-aging effect on the skin.

The leek may be included in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Has a polyphenol and the polyphenol has an excellent effect for removing harmful active oxygen. In addition, since the purple pigment of the branch contains a large amount of anthocyanin, which is an antioxidant, it has an antioxidant effect to prevent cell aging.

In addition, since the content of beta-carotene, potassium and vitamin is high, it is effective for skin-beauty.

Therefore, it is possible to produce beta-carotene, anthocyanin and various vitamins in the body of crickets by feeding the feed including branches into crickets, thereby producing functional crickets having antioxidant and anti-aging effects.

The above-mentioned branches may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Chungkookjang is known as a food to help the body release toxins and waste products. In addition, vitamin E contained in Chungkukjang has antioxidant action, which can prevent aging.

Therefore, it is possible to accumulate vitamin E in the body of crickets by feeding the crickets containing the chungkookjang to crickets, so that it is possible to produce functional crickets having antioxidant and anti-aging action and having an anti-aging effect on the skin.

The chungkukjang may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Cabbage contains vitamins A, B1, B2, C, K and other essential amino acids, lysine. In addition, the carotenoid component of cabbage has antioxidant action to prevent skin aging, and vitamin C has an effect of preventing collagen formation and helping to form collagen.

The cabbage may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

It contains vitamin A, vitamin B1, vitamin B2, vitamin C, iron, calcium, and carotene, so it has elasticity and shine, so it is effective for skin beauty. Because it is rich in various vitamins, it is effective for antioxidant and anti-aging action, so nutritional ingredients that have skin-beauty effect can be accumulated in the cricket body.

Therefore, it is possible to produce functional crickets that have antioxidant and anti-aging effects by feeding the feed including Artemisia to crickets to prevent skin aging.

The horse mugwort may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

Kale is called Omega 3 vegetable oil and contains a large amount of melatonin, which inhibits the production of active oxygen during cell metabolism, and has anti-aging, immunity and skin-care benefits.

Kale contains twice as much vitamin C as oranges, and three times as much calcium as milk. Among the green vegetables, the content of vitamin A is very high. 100 g of kale contains 3300 IU of vitamin A. This is about 100 times more than the vitamin A in cabbage, which is equivalent to 133% of the daily recommended amount.

As described above, kale which is rich in various vitamins also contains omega-3 unsaturated fatty acid, so it has an antioxidant and anti-aging effect and is very beneficial for enhancing skin beauty.

Therefore, the feed containing kale can be fed to crickets to produce antioxidant, anti-aging action to produce functional crickets with anti-aging effects.

The kale may be contained in an amount of 7 to 8 parts by weight based on 100 parts by weight of the whole feed.

The feed composed of these materials may be prepared by mixing the respective ingredients intact or by pulverizing the mixture into 50 to 100 mesh and then preparing the mixture in the form of a pellet, and the form of the feed to be fed is not limited thereto.

In addition, the feed is fed to cricket three times a day, and can be fed at 0.005 to 0.02 g per bird per feeding.

In addition, the cricket can be raised for 2 to 3 months in an environment controlled at a temperature of 27 to 32 ° C and a humidity of 60 to 75%. In this case, the light is illuminated for 10 hours on a day, It can be adjusted to darkness and raised.

Crickets raised as above have an effect of increasing the content of vitamin C, E, lycopene, beta-carotene and alpha-carotene, which are essential for prevention of aging and collagen formation, compared to crickets raised by feeding general feeds. In particular, it includes 0.75 to 0.85% of methionine, which is a protein amino acid of cricket, and 3.80 to 4.20% of lysine. This is 25 to 30% more than general cricket.

As a result, the cricket has an antioxidant and anti-aging effect, which is an excellent effect in improving skin beauty.

The crickets raised in the above can be used as food only by the cricket itself, and the crickets can be manufactured in various forms such as powder, capsule, ring, and extract.

Hereinafter, the cosmetic composition containing crickets will be described as follows.

As described above, crickets raised to improve antioxidation, anti-aging, and skin-beauty improving effects can be extracted by various methods to produce a cosmetic composition improved in antioxidant, anti-aging and skin-beauty improving effects. At this time, the method for extracting various active ingredients of the cricket can be performed by a method used in the art. In this case, the solvent of the extract may be one or a mixture of two or more selected from the group consisting of water, ethanol, butylene glycol, propylene glycol, dipropylene glycol and glycerin.

For example, 500 g of crickets enhanced in antioxidation, anti-aging and skin-beauty improving effects were subjected to hot-water extraction in 4,000 to 6,000 ml of distilled water, the resulting hot-water extract solution was concentrated at a ratio of 1/50 to 1/60, If the effective concentration is more than 350 mg / kg, the effect of improving antioxidant, anti-aging and skin beauty may be insignificant compared to the increase amount, and if the effective concentration is more than 250 mg / Kg, there is a problem that the effect of improving antioxidation, anti-aging and skin beauty may be lowered.

Accordingly, the cosmetic composition containing crickets raised as described above can be usefully used because it has effects of skin care, protection of human skin, antioxidation, skin improvement, skin whitening, exfoliation, and improvement of atopic skin.

The cosmetic composition containing crickets may contain 1 to 15 parts by weight, preferably 2 to 10 parts by weight, of the extract extracted from crickets of the present invention in 100 parts by weight of the cosmetic composition which is usually used.

If the cricket extract is less than 1 part by weight, it is difficult to achieve the excellent effects of antioxidation and anti-aging for the purpose of the present invention. If the cricket extract is more than 15 parts by weight, troubles due to high nutrition may occur .

In addition, in the case of the cosmetic composition according to the present invention, in addition to the extract containing the active ingredient of the cricket produced according to the method of the present invention, it may contain components conventionally used in cosmetic compositions such as antioxidants, stabilizers, , Vitamins, higher fatty acids, sunscreens, antioxidants, preservatives, colorants, pH adjusting agents, pigments and flavoring agents.

The cosmetic composition may include at least one selected from vegetable oils, mineral oils, silicone oils, and synthetic oils. In addition, 0.1 to 5 parts by weight of a surfactant, a higher alcohol and the like may be added to reinforce the emulsifying ability.

Further, 0.001 to 5 parts by weight of at least one thickening agent such as carbomer, xanthan gum, bentonite and the like may be further added to adjust the viscosity or hardness.

The cosmetic composition according to the present invention can be prepared into any formulation conventionally produced in the art. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays But is not limited thereto.

The cosmetic composition containing crickets of the present invention contains as active ingredients a large amount of vitamins C, E, lycopene, beta-carotene and alpha-carotene, which are essential for prevention of aging and collagen formation. The protein amino acids methionine and lysine Therefore, it is effective for skin aging and wrinkle prevention caused by oxidation of cells by active oxygen.

Therefore, the cosmetic composition containing crickets of the present invention exhibits an excellent effect in antioxidation and anti-aging action, and has an effect of skin improvement.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples. It will be apparent to those skilled in the art, however, that these examples and test examples are provided to further illustrate the present invention and that the scope of the present invention is not limited thereby.

<Examples> Crickets enhanced in antioxidant, anti-aging and skin-beauty improving effect

8 parts by weight of rice, 8 parts by weight of spinach, 8 parts by weight of carrot, 8 parts by weight of lettuce, 8 parts by weight of bean sprouts, 8 parts by weight of Chinese cabbage, 8 parts by weight of corn, 8 parts by weight of wormwood, , 8 parts by weight of branches, 8 parts by weight of chungkukjang, 8 parts by weight of cabbage, 8 parts by weight of arsinus and 8 parts by weight of kale were fed to crickets three times a day, 0.01 g / The crickets were kept at 30 ℃ for 60 days and light for 10 days and 14 hours for 80 days.

<Comparative Example> A general cricket

Normal feeds were fed and fed in a controlled environment under the same conditions as in the above examples.

<Test Example 1> Nutritional analysis of crickets improved in antioxidant, anti-aging and skin-beauty improving effect

The crickets developed by the method of the present invention in the present invention are excellent in antioxidant, anti-aging and skin-beauty improving effects as compared with the crickets of the comparative example raised with general feed. These results were confirmed by the fact that the crickets with nutrients that promote antioxidation, anti - aging and skin - beauty enhancement were promoted. The above results are compared with the nutritional components of crickets of the examples and comparative examples.

The analysis results of the nutritional components of the crickets of the examples and comparative examples were obtained by using an experimental equipment analyzer of the habit of joint laboratory experiment in Chonnam National University and the experiment method was as follows according to the result of the standardized experimental method in accordance with the analysis method of the university analysis center Respectively. Also, since crickets are living organisms, the exact concentration is averaged within an error range of ± 5 to 8 as a result of repeated experiments.

The results of the nutritional components of crickets analyzed by the above method are shown in Fig. 2, Fig. 3, Fig. 4 and Fig. 4 when the concentration of the nutritional components analyzed in the comparative example is taken as 100% Respectively.

As shown in FIG. 2, it was confirmed that vitamins, which are one of the components emphasized in many commercially available skin-improving cosmetics, contain less vitamins than common cosmetics in crickets raised as in the above example.

High-performance liquid chromatography (HPLC) was used for vitamin content analysis. The apparatus was a model of LC-10Avp (High Performanoe Liquid Chromatography, Shinadzu (UA)), and its quantitative comparison was analyzed by standard solution.

As shown in FIG. 2, the crickets cultivated by the above example proved to increase the amount of vitamins only by the method of the embodiment without addition of the synthesized nutrients. The effect of antioxidation, anti-aging and skin improvement was not enhanced in the crickets of the comparative examples, but the effect of antioxidation, anti-aging and skin improvement was enhanced in the crickets of the above examples. .

In Fig. 3, contents of minerals contained in crickets of Examples and Comparative Examples were analyzed. For the above analysis, an inductively coupled plasma-optical emission spectrometer (ICP-OES) of Perkin Elmer's OPTIMA 7300 DV model, which is owned by Chonnam University joint lab habits, was used.

In Fig. 4, contents of amino acids contained in the crickets of the examples and comparative examples were analyzed. For this analysis, S-433H AAAA (Automatic Amino Acid Analyzer, SYKAM) was used.

As a result of analyzing the vitamin minerals protein as described above, it was confirmed that the sodium (sodium) content was lowered, but the components that helped to improve the antioxidant, anti-aging and skin beauty were increased remarkably.

The cricket of the above example was analyzed by gas chromatography (GC-17A) of Shimadzu Co. (UA) to show that the content of omega-3 was 120% higher than that of the comparative example and the content of unsaturated fatty acid increased omega- .

As a result of the comparison of the above test results, crickets raised to improve the antioxidation, anti-aging and skin-beauty improvement effects developed in the present invention were analyzed to have sufficient contents of vitamins, minerals, amino acids and fatty acids I could.

As shown in the results of Test Example 1 above, crickets have high fat (21.8% in fat) and high protein (52.81% in protein) and contain unsaturated fatty acids when compared to the total weight.

In addition, the crickets of the examples showed that the content of methionine and lysine was improved by about 30% as compared with the crickets of the comparative example, and the contents of other amino acids and fatty acids were also improved.

In addition, it can be seen that the crickets of the Examples have an improved content of components of vitamins C, E and beta carotene necessary for the function of forming collagen and preventing aging than crickets of the comparative examples. Therefore, it can be seen that anti-aging, antioxidative action and skin cosmetic effect can be enhanced.

&Lt; Test Example 2 >

The cosmetic composition containing the crickets of the above-mentioned Example was tested with a cosmetic material by a person to be tested, and the result was confirmed as moisture, oil, and skin condition (wrinkle improvement).

The above test was conducted in Jeonju Vision College of Beauty and Health. The test results were quantified by the established authoritative verification method.

The instrument used for the above verification was a robo skin analyzer CS50 (ROHS skin analyzer CS50) and a flow meter (HL-611).

The face front, left, right and side were measured with the above Roboskin Analyzer CS50, and the values were calculated by checking the pores, skin tone, pigmentation, flushing, and wrinkles.

Using the above-mentioned water level meter, the values of the water content in the forehead forehead, center of the jaw, center of the left ball, and center of the right ball were calculated, and the confidence rate of the machine was 98%.

end. Test method of experimental group.

a) Check the skin condition before the test. All subjects were treated with the same cleanser (EM foam cleansing). After 3 minutes, pre-skin diagnosis was performed with a ROBOS SKIN ANALYSIS CS50 and a HL-611.

b) In the facial pack process, 20 g of dried cricket powder and 20 ml of distilled water were mixed, and the gauze was soaked in distilled water to remove the water and then put on the face, and the cricket face pack mixture was applied thereon.

After the skin was left to stand for 20 minutes, the pack material was removed with a warm towel, and the skin was cleaned with a skin. After 3 minutes, the skin was diagnosed with a robo skin analyzer CS50 and a water content analyzer (HL-611) Respectively.

c) After 24 hours of the experiment, all subjects were asked to use the same cleanser (EM foam cleansing), followed by 3 minutes of pre-skin diagnosis using a ROBS skin analyzer CS50 (ROBS skin analyzer CS50) and a water content meter (HL-611) .

I. Test method of control group

a) Check the skin condition before the test. All subjects were treated with the same cleanser (EM foam cleansing). After 3 minutes, pre-skin diagnosis was performed with a ROBOS SKIN ANALYSIS CS50 and a HL-611.

b) The face pack process was applied with a foreign brand mask pack (S company).

After the skin was left to stand for 20 minutes, the pack material was removed with a warm towel, and the skin was cleaned with a skin. After 3 minutes, the skin was diagnosed with a robo skin analyzer CS50 and a water content analyzer (HL-611) Was performed

c) After 24 hours of the experiment, all subjects were asked to use the same cleanser (EM foam cleansing), followed by 3 minutes of pre-skin diagnosis using a ROBS skin analyzer CS50 (ROBS skin analyzer CS50) and a water content meter (HL-611) .

Such a test procedure is shown in FIG.

The test results were measured on the basis of Table 1 and plotted on FIG. 5, FIG. 6, and FIG. 7.

Figure pat00001

As shown in the schematic diagram of FIG. 1, the test was conducted in the same manner by applying the steam towel of the room temperature of 20 ° C, the humidity of 50%, the distilled water and the temperature of 38 ° C uniformly in both the test group and the control group.

In FIG. 5, the water content was measured by dividing the female of the twenties, thirties, and forties into the experimental group and the control group, respectively. As a result, it was confirmed that the moisture was not inferior to the control group in which the commercial cosmetic pack was applied. The above results indicate that the pack of the experimental group has a strong moisturizing power even without any other chemical treatment.

In Fig. 6, the oil status of the experimental group and the control group was measured. As a result, there was a slight difference according to the state before use of the control group of the experimental group, but the oil retention that was not inferior to the control group which was generally commercialized cosmetic pack was shown. Considering that the above composition is a product that has not been formulated, excellent results than the control group can be expected in the future through various formulations.

In FIG. 7, it was confirmed that the test group was more effective than the control group in which the commercial cosmetic pack was applied, as a result of measuring the degree of wrinkle improvement in the skin condition. This is an environmentally friendly composition, and it was confirmed that the result of the experimental group shows that the penetration is high, the spreadability is good, and the skin trouble does not occur.

In the above test, the clinical test of facial cosmetic pack using environmentally friendly cricket dried powder was used as basic raw material for future cosmetics based on the nutritional ingredients related to skin beauty effect of cricket. The raw materials were powdered and used without being varied.

As a result of the test, it was confirmed that the clinical results were better or not inferior when compared with the control group using the commercialized pack. These results indicate that the crickets, which are the natural ingredients used in the present invention, are improved in antioxidative, anti-oxidant and skin-beauty improving effects, and can not be completely decomposed or purified by moisture or water It is possible to confirm that it is a good raw material that can solve the problems causing environmental pollution such as water pollution.

The test results showed that the crickets, which were used for the antioxidant, antioxidant and skin care improving effects as described in Test Example 2, were smooth and unexpected and had good adhesion even when they were not formulated. The test results were confirmed to be non-irritating. In addition, it has been highly appreciated for the future development of cosmetic packs and cosmetics products due to the popularity of natural beauty and popular taste and the effect of being not remodeled.

In Test Example 2, it was confirmed that the effect of improving the effect of constantly improving the antioxidant, anti-aging and skin beauty in the raw material without using the formulation and the commercialization for the above-mentioned invention by using the pack containing the distilled water and the crushed cricket Therefore, it can be seen that the development of cosmetics to which the product of the present invention is added can make it more friendly and effective.

Accordingly, it has been confirmed that the cosmetic composition containing crickets of the present invention exhibits an excellent effect on antioxidative and anti-oxidative effects and has an effect of improving the skin. The above-mentioned examples and test examples show that the scope of the present invention is limited It does not.

Claims (7)

7 to 8 parts by weight of tomato, 7 to 8 parts by weight of spinach, 7 to 8 parts by weight of carrot, 7 to 8 parts by weight of lettuce, 7 to 8 parts by weight of bean sprouts, 7 to 8 parts by weight of Chinese cabbage, 7 to 8 parts by weight of horse mackerel, 7 to 8 parts by weight of leek, 7 to 8 parts by weight of leek, 7 to 8 parts by weight of kernels, 7 to 8 parts by weight of chungkukjang, 7 to 8 parts by weight of cabbage, 8 parts by weight of a cricket which has been fed with an improved antioxidant, anti-aging and skin-care improving effect. The method according to claim 1,
The feed,
The crickets are fed to crickets three times a day, and fed 0.005 to 0.02 g per each feed per feeding. The crickets are improved in antioxidant, anti-aging and skin-care improving effects.
The method according to claim 1,
The cricket includes:
The content of amino acid or unsaturated fatty acid of any one of methionine, lysine, cysteine, threonine, serine, glutamic acid, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, histidine, arginine and proline is increased A cricket containing enhanced antioxidant, anti-aging and skin-healing effects.
A cosmetic composition containing crickets improved in antioxidative, anti-aging and skin-beauty improving effects according to any one of claims 1 to 3 can be used as cosmetic lotion (skin lotion), nutritional lotion, nutritional cream, massage cream, pack, And nutritional essence, wherein the effect of improving antioxidation, anti-aging and skin-beauty is improved. An anti-oxidant, anti-aging and skin-beauty improving effect of any one of claims 1 to 3, wherein the cricket contains crickets containing the crickets as a powder for cosmetic pack, &Lt; / RTI &gt; 6. The method according to any one of claims 1 to 5,
A cosmetic composition containing crickets which is produced by incorporating the above-mentioned effective ingredient into a pack made of a sheet-like or sleeping-type mask pack or cream formulation, wherein the effect of improving antioxidant, anti-aging and skin-
Antioxidant, anti-aging and skin-care improvement which can be ingested in the form of powder, capsule, ring, extract or the like containing cricket enhanced by antioxidation, anti-aging and skin-beauty improving effect of any one of items 1 to 3 A cricket improved in effect and a cosmetic composition containing the same.

KR1020160012240A 2015-02-05 2016-02-01 Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition comprising the same KR101809451B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2016/001161 WO2016126096A1 (en) 2015-02-05 2016-02-03 Crickets having increased antioxidant, antiaging and skin care effects, and cosmetic composition containing same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020150017753 2015-02-05
KR20150017753 2015-02-05

Publications (2)

Publication Number Publication Date
KR20160096548A true KR20160096548A (en) 2016-08-16
KR101809451B1 KR101809451B1 (en) 2018-01-18

Family

ID=56854511

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020160012240A KR101809451B1 (en) 2015-02-05 2016-02-01 Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition comprising the same

Country Status (1)

Country Link
KR (1) KR101809451B1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190013418A (en) * 2017-12-21 2019-02-11 한국 한의학 연구원 Composition for improving skin wrinkle or skin moisturizing comprising Gryllus bimaculatus extract as effective component
KR20190013544A (en) * 2017-07-28 2019-02-11 한국 한의학 연구원 Composition for improving skin wrinkle or skin moisturizing comprising Gryllus bimaculatus extract as effective component
KR102068521B1 (en) * 2018-10-29 2020-01-21 대한민국(농촌진흥청장) Cosmetic composition comprising teleogryllusine 2 peptide for preventing or improving acne or atopic dermatitis
US10959933B1 (en) 2020-06-01 2021-03-30 The Procter & Gamble Company Low pH skin care composition and methods of using the same
US11110049B2 (en) 2017-06-23 2021-09-07 The Procter & Gamble Company Composition and method for improving the appearance of skin
KR20220077991A (en) * 2020-12-02 2022-06-10 재단법인 전주농생명소재연구원 Composition for anti-inflammation and anti-wrinkle comprising fermentative product of Gryllus bimaculatus extract as effective component
US11583488B2 (en) 2020-06-01 2023-02-21 The Procter & Gamble Company Method of improving penetration of a vitamin B3 compound into skin
US11622963B2 (en) 2018-07-03 2023-04-11 The Procter & Gamble Company Method of treating a skin condition

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230067774A (en) 2021-11-09 2023-05-17 농업회사법인 누리(주) Health care food composition for protein supplement

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030085837A (en) 2002-05-02 2003-11-07 기아자동차주식회사 Hanger rubber of exhaust device for vehicles
KR20140146415A (en) 2013-06-17 2014-12-26 이경래 Auxiliary apparatus for willowing machine

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030085837A (en) 2002-05-02 2003-11-07 기아자동차주식회사 Hanger rubber of exhaust device for vehicles
KR20140146415A (en) 2013-06-17 2014-12-26 이경래 Auxiliary apparatus for willowing machine

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11110049B2 (en) 2017-06-23 2021-09-07 The Procter & Gamble Company Composition and method for improving the appearance of skin
KR20190013544A (en) * 2017-07-28 2019-02-11 한국 한의학 연구원 Composition for improving skin wrinkle or skin moisturizing comprising Gryllus bimaculatus extract as effective component
WO2019022478A3 (en) * 2017-07-28 2019-04-25 한국 한의학 연구원 Composition containing gryllus bimaculatus extract as active ingredient for improving skin wrinkles or moisturizing skin
KR20190013418A (en) * 2017-12-21 2019-02-11 한국 한의학 연구원 Composition for improving skin wrinkle or skin moisturizing comprising Gryllus bimaculatus extract as effective component
US11622963B2 (en) 2018-07-03 2023-04-11 The Procter & Gamble Company Method of treating a skin condition
KR102068521B1 (en) * 2018-10-29 2020-01-21 대한민국(농촌진흥청장) Cosmetic composition comprising teleogryllusine 2 peptide for preventing or improving acne or atopic dermatitis
US10959933B1 (en) 2020-06-01 2021-03-30 The Procter & Gamble Company Low pH skin care composition and methods of using the same
US11583488B2 (en) 2020-06-01 2023-02-21 The Procter & Gamble Company Method of improving penetration of a vitamin B3 compound into skin
US11911498B2 (en) 2020-06-01 2024-02-27 The Procter & Gamble Company Low pH skin care composition and methods of using the same
KR20220077991A (en) * 2020-12-02 2022-06-10 재단법인 전주농생명소재연구원 Composition for anti-inflammation and anti-wrinkle comprising fermentative product of Gryllus bimaculatus extract as effective component

Also Published As

Publication number Publication date
KR101809451B1 (en) 2018-01-18

Similar Documents

Publication Publication Date Title
KR101809451B1 (en) Crickets for improved antioxidant, antiaging activity, skin beauty and cosmetic composition comprising the same
US6391345B1 (en) Cranberry seed oil, cranberry seed flour and a method for making
Balboa et al. Cosmetics from marine sources
JP4732854B2 (en) Peroxisome proliferator-responsive receptor activator
JP2003095913A (en) Cosmetic composition or food and drink
JP4100911B2 (en) Glutamate decarboxylase activator
Pinto et al. Cosmetics—Food waste recovery
JP2004107286A (en) Cosmetic composition and food composition for beauty and health
KR101730852B1 (en) Cosmetic composition comprising fermented color food extracts
KR20120108930A (en) Antioxidants and skin cosmetics
JP4109099B2 (en) Screening method for active ingredient of hair restorer and its use
JP2003081868A (en) Stress inhibitor
KR102151044B1 (en) Cosmetic Composition Containing Vitamin C Stabilized by Moringa Seed Bark And Method Of The Same
KR20180044044A (en) Soap Containing Fermented Medicinal Herbs Include Ginseng, and Method for Manufacturing the Same
KR20180010743A (en) Cosmetic composition for preventing skin aging comprising watermelon rind juice as effective component
JP2002212046A (en) Cosmetic composition
KR20180024141A (en) Cosmetic composition for SPA comprising natural mixture as effective component
KR20220028612A (en) Cosmetic composition antimicroboal, antioxidation, whitening or anti-inflammation comprising artemisia capillaris thunberg and olive extract
KR20210119680A (en) Composition for enhancing skin barrier comprising eggshell membrane powder
KR100515975B1 (en) Desired soap with ginsenoside saponin and germanium
KR101690001B1 (en) Method for preparing a cosmetic composition containing the whitening-9 complex
WO2016126096A1 (en) Crickets having increased antioxidant, antiaging and skin care effects, and cosmetic composition containing same
JP5947072B2 (en) Antioxidant
JP2019182786A (en) Anti-glycation composition
JP2003063972A (en) Antiallergic agent

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
J202 Request for trial (for correction)
J301 Trial decision

Free format text: TRIAL NUMBER: 2018105000021; TRIAL DECISION FOR CORRECTION REQUESTED 20180213

Effective date: 20190111

G170 Publication of correction