KR20160056772A

KR20160056772A - Method for production of fungi mycelia complex with Inonotus obliquus, Phellinus linteus and parassis Crispa

Info

Publication number: KR20160056772A
Application number: KR1020150083660A
Authority: KR
Inventors: 박종례
Original assignee: 박종례
Priority date: 2014-11-12
Filing date: 2015-06-12
Publication date: 2016-05-20
Also published as: KR101652035B1

Abstract

The present invention relates to a production method of a composite mushroom mycelium. The production method of the composite mushroom mycelium comprises a step of culturing mycelia of Inonotus Obliquus, Phellinus linteus and Sparassis crispa in a culture medium prepared by mixing barley, oat, and Lentinula edodes. Accordingly, the mycellia of Inonotus Obliquus, Phellinus linteus and Sparassis crisp are mixed and can be cultured under the same conditions. In addition, The production method cultures the composite mushroom mycelium which contains all β-glucans separately derived from Inonotus Obliquus, Phellinus linteus and Sparassis crisp so the composite mushroom mycelium is effective.

Description

{Abstract: The present invention relates to a method for producing a mushroom mycelium comprising a mushroom, a mushroom and a mushroom of a fungus,

The present invention relates to a method of producing a composite mushroom mycelium, more specifically, a difference in mixing barley, oats, and shiitake mushroom (Inonotus Obliquus), Phellinus linteus (Phellinus linteus) and blossoms mushrooms (Sparassis Crispa) mycelium medium To thereby produce a complex mushroom mycelium containing all the beta-glucans derived from each mushroom.

The mushrooms belonging to the basidiomycetes have high nutritional value and unique flavor and taste, which are not only suitable for edible use but also exhibit various pharmacological effects. Currently, mushrooms are considered to be the most successful example of converting agricultural and forestry byproducts to human food, and the total production of edible mushrooms worldwide is estimated at 3,763,000 tons in 1991.

Among the edible mushrooms, Inonotus Obliquus is a mushroom known to have an anticancer effect and consists of 40 to 50 components. Representative components of the mushroom are chromogen complex, beta-glucan, polysaccharide, polyphenol, oxyphenol carboxylic acid, lignin, magnesium magnesium, aluminum oxide, iron, calcium, carboxy acid, cellulose, steroids, organic acids (up to 4.5% (Si, Fe, Ca, Ma, Cu, Mg, Na, Zn). In particular, the content of β-glucan is 56.68 mg per 10 g of mushroom, and it is known to be superior to mushroom and agaricus mushroom (Japan Hokkaido Food Processing Center, Jun. 27, 2003).

Phellinus linteus is a Chinese term originating from mulberry and pressed yellow, but its academic meaning and general meaning are slightly different, and there are many kinds of edible mushrooms. Comprehensively refers to various kinds of mud mushrooms. According to Oriental medicine, mushrooms are known to be effective for cervical cancer, chronic gastroenteritis, stomach cancer, and colon cancer. In addition, the mushroom contains amino acids, hydrocarbons, fatty acids, enzymes, potassium, calcium and vitamin B as well as protein polysaccharides. It contains beta-glucan and improves immune function.

Sparassis Crispa is one of the parasitic mushrooms in Korean coniferous forest resources. It is a high temperature mushroom that grows mainly in pine, pine, larch, and fir from May to August. In early 1997, Japanese high school teacher Fukushima started to develop the sawdust cultivation method, and interest in medicinal value began to be born. Since then, it has been confirmed that beta-glucan is contained in a large amount, and it has received worldwide attention.

As such, chaga mushroom, shiitake mushroom and shiitake mushroom contain a large amount of beta-glucan, which has immunity enhancing action, and contains a large amount of useful components in the human body. Therefore, the combination of Chaga mushroom, Situ mushroom and Chesnut mushroom seems to increase the useful ingredients.

However, in order to use it, a mushroom which is a fruiting body should be sampled and used. Since mushroom fruiting body does not easily reproduce in a natural state, there is a problem that resource abuse and destruction of ecosystem are caused by abuse of harvesting when natural mushroom is collected. It is known that complex culture of mushroom mycelium is difficult because strong mushroom mycelium kills all mycelial hyphae when cultivated mushroom mycelium of different kinds, although there is movement to combine mycelium exhibiting equivalent effect with fruit body to solve this problem.

Therefore, there is a need to study a culture method capable of complex culturing mycelium of mushroom bacteria against the mushrooms.

Korean Patent No. 10-1254503 (a method of producing mycelia of carob bean using buckwheat) discloses that mycelium extracted from sclerotia, which is a mushroom fruiting body, is used as a medium for the buckwheat, Desc / Clms Page number 2 > method for producing mushroom mycelium. Korean Patent Laid-Open No. 10-2002-0027970 (a method of cultivating mushroom mycelium using cereals and the product thereof), inoculated with edible and medicinal mushroom mycelium in a solid medium containing grains, And a method for culturing mycelia of mycelia producing a large amount of self-decomposition products containing an anticancer component and a health promoting substance by inducing degradation, and products thereof.

The present invention relates to the use of Inonotus Obliquus , Phellinus linteus and Sparassis Crispa ) mycelium, and thus, it is intended to produce a complex mushroom mycelium containing all the β-glucans derived from each mushroom.

In order to achieve the above object, the present invention provides a method for producing a medium comprising: (A) preparing a culture medium by mixing 50 to 80% by weight of barley, 10 to 40% by weight of oats, and 5 to 30% by weight of an altitude; (B) adding calcium carbonate (CaCO ₃ ) to the medium, mixing and sterilizing the medium after the step (A); (C) a step of inoculating the mushroom mycelium and culturing for 20 to 30 days after the step (B); And (D) drying and pulverizing the cultured mushroom mycelium at a temperature of 50 to 80 DEG C after the step (C), wherein the mushroom mycelium of step (C) is obtained by dehydrating boron barley, Adding (CaCO ₃ ) and sterilizing (a); And step (b) of inoculating the sclerotial cells separated from the mushroom, the mushroom and the mushroom after the step (a) and culturing the mushroom for 20 to 30 days, respectively, And a method for producing the same. In this case, the mushroom mycelium of step (C) preferably further comprises step (C) of drying and pulverizing at 50 to 80 ° C after step (B).

According to the production method of the present invention, it is possible to co-cultivate the mushroom, the mushroom, and the mushroom mycelium, thereby obtaining all of the beta-glucan from the mushroom, the mushroom and the mushroom.

Hereinafter, the production method of the present invention will be described in detail.

&Lt; Step (A): Step of producing medium >

In this step, medium is prepared by mixing barley and oats with cereal mushrooms. The medium is prepared by mixing 50 to 80% by weight of barley, 10 to 40% by weight of oats and 5 to 30% by weight of oak.

The cereal medium refers to a medium composed of at least one selected from edible rice, brown rice, barley, wheat, corn, barley and oats. When cereals are used, the necessary carbon and nitrogen sources are added It does not need to be added. That is, carbon and nitrogen sources are simultaneously satisfied by carbohydrates, proteins and amino acids contained in cereals.

In the present invention, barley and oats are selected as cereals and mushroom is added thereto to form a culture medium. Through this, it is possible to mix and co-cultivate mycelia of mushroom, mushroom and mushroom mycelia, (β-glucan) can be produced all at once.

On the other hand, β-glucan is a substance which exists in the cell wall of yeast, mushrooms, cereals, etc. and has immunity enhancing action. Β-glucan is a glucose polymer having a basic structure of β-glycosidic bonds at positions 1 and 3 of the glucose unit, and its structure and physicochemical properties are different depending on the position where glucose is bonded. Beta Glucan (β-glucan) does not directly attack cancer cells, but it activates immune function of normal human cells by nonspecific immune reaction, inhibits the proliferation and recurrence of cancer cells, activates macrophages, and enters cancerous cells It stimulates the secretion of various cytokines, thereby activating the immune functions of immune T cells and B cells. In addition, β-glucan has excellent blood glucose lowering effect and blood cholesterol reducing effect, and has been reported to have anti-obesity effect by inhibiting body fat formation and accumulation by improving lipid metabolism.

&Lt; Step (B): pH adjustment and sterilization step >

In this step, calcium carbonate (CaCO ₃ ) is added to the medium prepared in the step (A) and mixed and sterilized. At this time, calcium carbonate (CaCO ₃ ) is preferably added to adjust the pH of the medium to 6 to 7, and sterilization is preferably performed for 1 to 3 hours.

&Lt; Step (C): Inoculation of mycelium and cultivation step >

This step is a step of inoculating mushroom mycelium and culturing for 20 to 30 days after the step (B). Preferably, the mushroom mycelia is inoculated at a rate of 10 to 30 parts by weight per 100 parts by weight of the medium prepared in the step (A). Mycelium is a group of mycelium that grows or branches. It has a certain area in the nutrients, feces, and natural substrates of fungi.

In step (C) of the present invention, the mushroom mycelium is preferably obtained by dehydrating barley called water, adding calcium carbonate (CaCO ₃ ) and sterilizing it; And 20 to 40 parts by weight of sclerotia isolated from a mushroom, a mushroom and a mushroom after the step (a) are inoculated in an amount of 3 to 5: 2 to 4: 2 to 4, It is preferable to use the one obtained from step (b) of culturing for 20 to 30 days. When the above range is satisfied, it is possible to smoothly cultivate mushroom, mushroom and mushroom mycelium.

Further, it is preferable to further include step (c) of drying and pulverizing at 50 to 80 ° C after the step (b). If the temperature is higher than the above temperature range, beta glucan may be destroyed, which is undesirable. If the temperature is lower than the above range, drying may not be smooth and drying time may be long.

&Lt; Step (D): Drying and pulverization step >

In this step, the cultured complex mushroom mycelium is dried and pulverized at a temperature of 50 to 80 ° C after the step (C). In this case, the drying is preferably performed at 50 to 80 ° C for 3 to 4 hours or more. If the temperature is higher than the above temperature range, beta glucan may be destroyed, which is undesirable. If the temperature is lower than the above range, drying may not be smooth and drying time may be long.

The present invention also provides a food composition comprising the complex mushroom mycelium produced by the production method of the present invention. Examples of the target food include, but are not limited to, meat, cereal, caffeinated beverages, ordinary beverages, chocolate, dairy products, breads, snacks, confectionery, pizza, jelly, noodles, gums, ice cream, alcoholic beverages, no.

The complex mushroom mycelium of the present invention contains beta-glucan derived from mushroom-derived mushroom-derived mushroom-derived mushroom, and has various pharmacological effects and can contribute to health.

The present invention relates to a method for producing a mushroom mycelium by forming barley, oats and shiitake mushrooms in a medium suitable for growth of mushroom mycelium, and cultivating the mycelium of Inonotus Obliquus , Phellinus linteus and Sparassis Crispa This method has the advantage of obtaining all the β-glucans derived from each mushroom.

Fig. 1 is a beta-glucan content test report of the mycelium of mushrooms of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Manufacturing example 1 and Comparative Manufacturing Example 1 to 2: mushroom Cultivation of mycelium]

In this example, mushroom mycelium was cultured by inoculation of sclerotium isolated from chaga mushroom, mushroom mushroom and mushroom mushroom.

800 g of barley called water was dehydrated, and then calcium carbonate (CaCO ₃ ) was added and sealed in a container. The sealed container was sterilized in a high pressure sterilizer for 2 hours and then transferred to a clean room.

30 g (240 g) of 100 g of barley were inoculated into the sterilized barley in the order described in the following Table 1, and then transferred to a culture room to obtain 25 Lt; / RTI > After the incubation, it was dried at 80 DEG C for 4 hours or more and pulverized using a pulverizer to produce mushroom mycelium.

Mushroom sclerotia Situ mushroom Sclerotia Mushroom scarlet Production Example 1 4 3 3 Comparative Preparation Example 1 4 6 3 Comparative Production Example 2 4 3 6

(Unit: weight ratio)

As a result of the experiment, it was confirmed that the mycelia of chaga mushroom, shiitake mushroom, and shiitake mushroom were uniformly activated in Example 1, whereas the shiitake mushroom dominated in Comparative Example 1 and the shrub mushroom dominated in Comparative Example 2 .

Therefore, it was confirmed that the mixture should be mixed at a ratio of 4: 3: 3 in order to uniformly disperse the mycelium of mushroom, mushroom, and mushroom.

[ Example 1 ~ Comparative Example 2 to 3: Compound mushroom Cultivation of mycelium]

In this Example, mushroom mycelium prepared in Preparation Example 1 was cultured in a medium containing barley, oats, and altitude to culture the mushroom mycelium. Barley, oats, and altitudes were mixed in the formulations shown in Table 2 below, called water, and then dehydrated and mixed to prepare a medium.

barley oat Elevation Example 1 400 200 100 Comparative Example 1 400 400 100 Comparative Example 2 400 200 400

(Unit: g)

Then, calcium carbonate (CaCO ₃ ) was added to the medium, mixed, put into a container, and sealed. The sealed container was sterilized in a high pressure sterilizer for 2 hours and then transferred to a clean room.

In the clean room, the above-mentioned mushroom mycelium prepared in Preparation Example 1 was inoculated into the sterilized medium and cultured for 25 days.

After culturing, the cultured mycelium was dried at 80 DEG C for 4 hours or longer and pulverized to obtain a composite mycelia.

[ Experimental Example 1: beta-glucan content analysis]

In this Experimental Example, the content of beta-glucan in the complex mushroom mycelium obtained in the above Examples and Comparative Examples 1 and 2 was analyzed.

Beta-glucan content Example 1 162.5 Comparative Example 1 123.2 Comparative Example 2 114.1

(Unit: mg / g)

As a result of the experiment, as shown in Table 3, the content of beta-glucan was 162.5 mg / g in Example 1, 123.2 mg / g in Comparative Example 1 and 114.1 mg / g in Comparative Example 2 It was found that the content of beta-glucan of Example 1 was much higher than that of Comparative Examples 1 and 2.

On the other hand, in addition to the content of beta-glucan in Example 1, a further investigation was requested from Korea Research Institute of Chemical Technology (Korea Research Institute of Chemical Technology), which confirmed that the content of beta-glucan in the complex mushroom mycelium was 163.6 mg / g.

[Preparation Example 2: Preparation of food composition containing mushroom mycelium]

In this Example, a food composition comprising mushroom mycelium was prepared as follows.

(1) Manufacturing of wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and then the mixture was prepared into powder having a particle size of 60 mesh by a pulverizer. Black beans, black sesame seeds and perilla seeds were each steamed and dried by known methods, and then power distribution and pulverization were carried out to prepare powder having a particle size of 60 mesh. Thereafter, 30 wt% of brown rice, 15 wt% of yulmu, 20 wt% of barley, 9 wt% of glutinous rice, 7 wt% of perilla seed, 8 wt% of black soybean, 7 wt% of black sesame seed, 3 wt% 0.5% by weight of Ganoderma lucidum and 0.5% by weight of Sorbitol were mixed to prepare an electric wire.

(2) Production of chewing gum

Chewing gum was prepared by combining 20% by weight of a gum base, 76.9% by weight of sugar, 1% by weight of a flavor, 2% by weight of water and 0.1% by weight of a complex mushroom mycelium (Example 1).

(3) Candy manufacturing

Candies were prepared in a conventional manner by mixing 60% by weight of sugar, 39.8% by weight of starch syrup, 0.1% by weight of fragrance and 0.1% by weight of mycelium of complex mushrooms (Example 1).

(4) Manufacturing of biscuits

By weight of starch, 0.77% by weight of glucose, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonia, 0.17% by weight of sodium bicarbonate, 0.16% by weight of sodium bisulfite 1.45 weight% of rice flour, 0.0001 weight% of vitamin B1, 0.0001 weight% of vitamin B2, 0.04 weight% of milk fractions, 20.6998 weight% of water, 1.16 weight% of whole milk powder, 0.29 weight% 0.29% by weight of spray salt, 7.27% by weight of spray oil and 1% by weight of mycelia of composite mushrooms (Example 1) were mixed to prepare a biscuit by a conventional method.

(5) health drink manufacturing

0.0001 wt.% Of niacinamide, 0.0001 wt.% Of sodium riboflavin hydrochloride, 0.0001 wt.% Of pyridoxine hydrochloride, 0.001 wt.% Of inositol, 0.002 wt.% Of orthoacetic acid, 98.7362 wt.% Of water, And 1% by weight of mycelium (Example 1) were mixed to prepare a health drink.

(6) Production of sausages

A mixture of 65.18 wt% of pork, 25 wt% of chicken meat, 3.5 wt% of starch, 1.7 wt% of soybean protein, 1.62 wt% of salt, 0.5 wt% of glucose and 1.5 wt% of glycerin and 1 wt% Sausages were prepared by a conventional method.

(7) Health supplement manufacturing

50% by weight of complex mushroom mycelium (Example 1), 16% by weight of guar gum enzyme hydrolyzate, 0.01% by weight of vitamin B1 and hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, By weight and 1.85% by weight of corn starch were mixed together to prepare a refillable health supplement.

Claims

(A) preparing a culture medium by mixing 50 to 80% by weight of barley, 10 to 40% by weight of oats, and 5 to 30% by weight of an altitude;
(B) adding calcium carbonate (CaCO ₃ ) to the medium, mixing and sterilizing the medium after the step (A);
(C) a step of inoculating the mushroom mycelium and culturing for 20 to 30 days after the step (B);
(D) drying and pulverizing the cultured mushroom mycelium at a temperature of 50 to 80 DEG C after the step (C)
Wherein the mycelium of step (C) is obtained by dehydrating boron barley, adding calcium carbonate (CaCO ₃ ) and sterilizing it; And
(B) a step of inoculating the sclerotia isolated from the mushroom, the mushroom and the mushroom, respectively, and culturing the mushroom for 20 to 30 days after the step (a), and using the obtained mushroom mycelium Production method.

The method according to claim 1,
The mushroom mycelium of step (C)
(B) drying and pulverizing the mixture at 50 to 80 ° C after the step (b).

The method according to claim 1,
The mushroom mycelium of step (b)
Wherein the scutellum isolated from a mushroom, a mushroom, a mushroom and a mushroom is inoculated successively at a ratio of 3: 5: 2 to 4: 2 to 4: 20 to 40 parts by weight per 100 weight of the barley. .

A food composition comprising the complex mushroom mycelium produced according to the production method of claim 1.