KR20020027970A - Process for mycelial culture using grain and product thereof - Google Patents
Process for mycelial culture using grain and product thereof Download PDFInfo
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- KR20020027970A KR20020027970A KR1020000058805A KR20000058805A KR20020027970A KR 20020027970 A KR20020027970 A KR 20020027970A KR 1020000058805 A KR1020000058805 A KR 1020000058805A KR 20000058805 A KR20000058805 A KR 20000058805A KR 20020027970 A KR20020027970 A KR 20020027970A
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- mushroom mycelium
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- solid medium
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000007787 solid Substances 0.000 claims abstract description 37
- 239000012141 concentrate Substances 0.000 claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 208000035404 Autolysis Diseases 0.000 claims abstract description 10
- 206010057248 Cell death Diseases 0.000 claims abstract description 10
- 230000028043 self proteolysis Effects 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 230000001939 inductive effect Effects 0.000 claims abstract description 4
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- 238000001816 cooling Methods 0.000 claims abstract 2
- 238000007789 sealing Methods 0.000 claims abstract 2
- 230000001954 sterilising effect Effects 0.000 claims abstract 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 72
- 235000013339 cereals Nutrition 0.000 claims description 25
- 239000000843 powder Substances 0.000 claims description 23
- 239000000047 product Substances 0.000 claims description 15
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 240000005979 Hordeum vulgare Species 0.000 claims description 6
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 240000001417 Vigna umbellata Species 0.000 claims description 4
- 235000011453 Vigna umbellata Nutrition 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims description 4
- 230000007281 self degradation Effects 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 240000000599 Lentinula edodes Species 0.000 claims description 2
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 2
- 241000190021 Zelkova Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 240000008397 Ganoderma lucidum Species 0.000 claims 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 235000021109 kimchi Nutrition 0.000 claims 1
- 230000001093 anti-cancer Effects 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
- 238000000227 grinding Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 4
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 11
- 239000002994 raw material Substances 0.000 description 5
- 235000013376 functional food Nutrition 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 235000006468 Thea sinensis Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FYGDTMLNYKFZSV-WFYNLLPOSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,3s,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-WFYNLLPOSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108010029541 Laccase Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000002788 crimping Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 208000024386 fungal infectious disease Diseases 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
- A23L31/10—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/308—Foods, ingredients or supplements having a functional effect on health having an effect on cancer prevention
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/50—Concentrating, enriching or enhancing in functional factors
Abstract
Description
본 발명은 곡물을 이용한 버섯 균사체의 배양방법 및 그 산물에 관한 것이다. 더욱 상세하게는, 본 발명은 곡물을 함유하는 고체배지에 식용 및 약용버섯 균사를 접종하고 배양시킨 후 자가분해를 유도하여 버섯 균사체의 자가분해물을 생산하는 버섯 균사체 배양방법 및 그 산물에 관한 것이다.The present invention relates to a method for culturing mushroom mycelium using grains and products thereof. More specifically, the present invention relates to a mushroom mycelium culturing method and a product for inoculating and cultivating edible and medicinal mushroom mycelium on a solid medium containing grains to induce autolysis to produce autolysates of mushroom mycelium.
지금까지 식용 및 약용버섯을 식품 및 의약품의 원료로 이용하려면 버섯자실체를 재배하여 이용하거나 또는 버섯균사체를 액체배양한 후 추출 및 특정 성분을 분리, 정제하여 이용하였다. 그러나 상기와 같은 추출 및 특정 성분의 분리정제과정은 상당한 시간과 비용이 요구되는 문제점이 있었다.Until now, to use edible and medicinal mushrooms as raw materials for food and pharmaceuticals, mushroom fruiting bodies were used, or mushroom mycelium was liquid-cultured and extracted and used to separate and purify specific components. However, the above extraction and separation and purification process of a specific component has a problem that requires a considerable time and cost.
최근에는 곡물을 이용한 배지에 버섯균사를 접종하여 배양시킨 후 곡물배지를 건조하여 직접 식용으로 이용하고 있다. 그러나 상기 방법은 고체배지와 함께 버섯에 함유된 유용성분들을 이용해야 하므로 이용방법이 제한적이고 버섯에 함유된 유용성분들의 이용효율이 떨어지는 단점이 있었다.Recently, after inoculating mushroom mycelia on a medium using grains, the grain medium is dried and used directly for food. However, the method has a disadvantage in that the use method is limited and the use efficiency of the useful components contained in the mushroom is lowered, since the useful ingredients contained in the mushroom are used together with the solid medium.
본 발명자들은 상기와 같은 점에 착안하여 곡물을 함유하는 고체배지에 버섯 균사를 접종하고 배양한 후 자가분해를 유도시켜 항암성분을 비롯해 다양한 가수분해효소 분해물이 함유된 건강증진물질을 얻으므로써 본 발명을 완성하였다.In view of the above, the present inventors inoculate and incubate mushroom mycelia on a solid medium containing grains, and then induce self-degradation to obtain a health promoting substance containing various hydrolytic enzyme degradation products including anticancer components. Was completed.
따라서 본 발명의 목적은 곡물을 함유하는 고체배지에 식용 및 약용버섯 균사를 접종하고 배양한 후 자가분해(autolysis or self-digestion)를 유도하여 버섯 균사체의 자가분해물을 생산하는 버섯 균사체 배양방법을 제공함에 있다.Accordingly, an object of the present invention is to inoculate and incubate edible and medicinal mushroom mycelium on a solid medium containing grains to provide a mushroom mycelium culture method for producing autolysates of mushroom mycelium by inducing autolysis or self-digestion. Is in.
본 발명의 다른 목적은 상기 버섯 균사체 배양방법에 의해 얻어진 산물을 제공함에 있다.Another object of the present invention to provide a product obtained by the mushroom mycelium culture method.
본 발명의 상기 목적은 곡물을 함유하는 고체배지에 식용 및 약용버섯 균사를 접종하고 배양하여 버섯 균사체를 활착시킨 후 자가분해(autolysis or self-digestion)를 유도하여 기능성 식품 및 그 원료로 유용한 버섯균사체의 자가분해물을 얻으므로써 달성하였다.The object of the present invention is to inoculate and incubate edible and medicinal mushroom mycelium on a solid medium containing grains to cultivate the mushroom mycelium, and then to induce autolysis or self-digestion, which is useful as a functional food and its raw material. Achieved by obtaining the autolysate of.
이하, 본 발명의 구성 및 작용을 설명한다.Hereinafter, the configuration and operation of the present invention.
도 1은 곡물을 함유하는 고체배지에서 식용 및 약용버섯 균사체를 생산하는 방법을 공정별로 도시한 개략도이다.Figure 1 is a schematic diagram showing a process for producing a edible and medicinal mushroom mycelium in a solid medium containing grains.
본 발명은 쌀, 보리 등과 같은 곡물로 고체배지를 제조하는 공정; 제조된 고체배지에 버섯균을 접종하여 배양시키는 공정; 활착된 버섯균사체에 자가분해를 유도하는 공정; 상기 자기분해 유도공정에서 얻은 버섯균사체의 자가 분해물을 압착하여 여액을 얻는 공정 및; 상기 압착공정에서 남는 페이스트를 건조, 분말화하여 기능성 식품 및 그 원료를 제조하는 공정으로 구성된다.The present invention is a process for producing a solid medium from grains such as rice, barley; Inoculating the prepared bacteria to inoculate mushrooms; Inducing self-decomposition to the adherent mushroom mycelium; Obtaining a filtrate by compressing the autolysate of the mushroom mycelium obtained in the self-decomposition process; It consists of the process of manufacturing a functional food and its raw material by drying and powdering the paste which remain | survived in the said crimping process.
상기 곡물을 함유하는 고체배지에서 버섯 균사체의 배양방법을 공정별로 상세히 설명한다.The method for culturing mushroom mycelium in a solid medium containing the grains will be described in detail for each process.
제 1 공정: 곡물을 함유하는 고체배지 제조First step: preparing a solid medium containing grain
쌀무게 2∼3배의 물을 쌀에 가하여 정상적인 취사를 하거나 불린 후 이를 배양용기에 넣어서 배지로 사용하였다. 배양용기에는 잡균의 침입을 저지할 수 있는 뚜껑을 장착하여 이것을 고압증기 살균기에서 15 ∼ 25분 동안 멸균하였다. 멸균 처리된 배양용기를 꺼내어 냉각시켰다.Rice weight of 2-3 times the water was added to the rice to cook normally or soaked and put it into a culture vessel was used as a medium. The culture vessel was equipped with a lid capable of preventing invasion of various bacteria, which was sterilized for 15 to 25 minutes in an autoclave. The sterilized culture vessel was taken out and cooled.
본 발명에서 곡물을 함유하는 고체배지에 이용될 수 있는 곡물의 종류는 쌀, 찹쌀, 보리, 밀, 조, 차수수, 차기장, 율무, 팥, 옥수수 등이며, 배양용기는 병(bottle)모양으로 생긴 것이면 특별히 제한되지 않는다.In the present invention, the kinds of grains that can be used for the grain-containing solid medium are rice, glutinous rice, barley, wheat, crude, sorghum, tea-plant, yulmu, red beans, corn, etc., and the culture vessel is in the shape of a bottle. It is not particularly limited if it is formed as
제 2 공정: 버섯균사접종 및 배양Second Process: Mushroom Mycosis and Culture
상기 제 1 공정에서 냉각시킨 무균상태의 배양용기에 버섯균사를 무균작업대에서 접종하였다. 이때, 접종되는 버섯균은 아가 플레이트(agar plate)상에서 배양된 것으로 하였으나 통상 액체배양된 것을 사용하여도 무방하다. 접종 후 20 ∼ 30℃의 배양실에서 15 ∼ 30일간 배양하여 버섯 균사체를 활착시켰다.Mushroom mycelia were inoculated in a sterile workbench in a sterile culture vessel cooled in the first step. At this time, the inoculated mushroom bacteria were cultivated on an agar plate, but may be used as a normal liquid culture. After the inoculation, the mushroom mycelium was allowed to incubate for 15 to 30 days in a culture chamber at 20 to 30 ° C.
본 발명에서 곡물을 함유하는 고체배지에 접종할 수 있는 버섯 균사체는 운지, 영지, 치마, 느타리, 여름느타리, 표고, 팽이, 목이, 맛버섯 등으로 식용 및 약용으로 사용되는 버섯이면 특별히 제한되지 않는다.Mushroom mycelium which can be inoculated into a solid medium containing grains in the present invention is not particularly limited as long as it is a mushroom used for food and medicinal purposes such as fingering, ganoderma, skirts, zelkova, summer lodge, shiitake, top, thirsty, mushrooms .
제 3 공정: 버섯 균사체의 자가분해 유도Third Step: Induction of Autolysis of Mushroom Mycelium
상기 제 2 공정에서 활착시킨 버섯균사체가 담겨 있는 배양용기의 외부표면을 물리적으로 5 ∼ 20여 차례 두드린 후 45 ∼ 55℃에서 24 ∼ 48시간동안 처리함으로써 버섯균사체의 자가분해를 유도하였다.The outer surface of the culture vessel containing the mushroom mycelium slid in the second step was physically knocked 5 to 20 times and then treated at 45 to 55 ° C. for 24 to 48 hours to induce self-degradation of the mushroom mycelium.
제 4 공정: 분해물의 압착Fourth Process: Compression of Decomposition
상기 제 3 공정에서 버섯균사체의 자가분해에 의해 얻어진 버섯균사체의 자가분해물을 압착하여 여액을 얻은 후 이를 압착하고 감압농축기나 가열로 농축하여 농축액을 제조하였다.In the third step, the autolysate of the mushroom mycelium obtained by autolysis of the mushroom mycelium was compressed to obtain a filtrate, which was then compressed and concentrated by a vacuum concentrator or heating to prepare a concentrate.
제 5 공정: 페이스트의 건조분말Fifth Step: Dry Powder of Paste
상기 제 4 공정에서 버섯 균사체의 자가분해물 농축액을 제조하고 남은 페이스트를 건조하여 분말제품을 제조하였다.In the fourth process, the autolysate concentrate of the mushroom mycelium was prepared, and the remaining paste was dried to prepare a powder product.
이상, 상기 제 1 ∼ 5 공정은 도 1에 도시한 바와 같으며, 압착한 농축액과 농축액 제조 후 남은 페이스트로 제조된 분말제품은 기능성 식품 또는 의약품의 제조시 재료로 사용된다.As described above, the first to fifth processes are as shown in FIG. 1, and the powder product prepared from the compressed concentrate and the paste remaining after the concentrate is used is used as a material in the production of functional foods or pharmaceuticals.
본 발명의 방법에 의해 얻어진 버섯균사체의 자가 분해물에는 폴리사카라이드(polysaccharides), β-D-글루칸(β-D-glucan) 등의 항암성분, 셀룰라아제(cellulases), 락카아제(laccases), 리파아제(lipases), 폴리갈락투로나아제(polygalacturonases), 프로테아제(proteases) 등 수십 종의 가수분해효소들과 이러한 효소들에 의해 가수분해된 곡류의 여러 분해물질들이 존재할 뿐만 아니라 탈취제거 등 여러 가지 기능을 가진 성분들이 포함되어 있어 이를 기능성 식품 및 그 원료 제조에 이용할 수 있는 장점이 있다.The autolysates of the mushroom mycelium obtained by the method of the present invention include anti-cancer components such as polysaccharides, β-D-glucan, cellulases, laccases, lipase ( Dozens of hydrolases such as lipases, polygalacturonases and proteases, and various degradation products of grains hydrolyzed by these enzymes exist, as well as deodorizing Contains ingredients that have the advantage that can be used to manufacture functional foods and raw materials.
이하, 본 발명의 구체적인 방법을 실시예를 들어 상세히 설명하고자 하지만 본 발명의 권리범위는 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.
실시예 1: 쌀을 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 1 Mushroom Mycelial Culture and Product Production in Solid Medium Containing Rice
쌀 500g에 물 1.5L를 가수한 후 취사하여 얻은 밥 1.9kg을 배양용기에 투입하고 오토클래브에서 20분간 멸균하였다. 멸균시킨 배양용기에 운지버섯균사를 무균작업대(clean bench)에서 접종하였다. 접종 후 25℃에서 25일간 배양하여 버섯 균사체를 활착시키고 배양용기의 표면을 물리적으로 10여차례 두드린 후 50℃에서 36시간 처리하여 버섯균사체의 자가분해를 유도하였다. 자가분해에 의해 얻어진 버섯 균사체의 자가분해물을 압착기로 압착하여 여액 1.1L을 제조하고 남은 페이스트는 열풍건조하여 분말 300g을 얻었다. 상기 여액 1.1L는 100℃에서 20분간 가열농축하여 농축액 600mL을 제조하였다.After stirring 1.5 L of water in 500 g of rice, 1.9 kg of cooked rice was added to a culture vessel and sterilized for 20 minutes in an autoclave. The sterilized culture vessels were inoculated with fingering mushroom hyphae on a clean bench. After inoculation, the mushroom mycelium was cultivated at 25 ° C. for 25 days, and the surface of the culture vessel was physically knocked about 10 times, and then treated at 50 ° C. for 36 hours to induce autolysis of the mushroom mycelium. 1.1L of the filtrate was prepared by pressing the autolysate of the mushroom mycelium obtained by autolysis by the compactor, and the remaining paste was hot-air dried to obtain 300g of powder. 1.1L of the filtrate was concentrated by heating at 100 ° C. for 20 minutes to prepare 600 mL of concentrate.
실시예 2: 찹쌀을 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 2: Mushroom Mycelium Culture and Production of the Products in Solid Medium Containing Glutinous Rice
찹쌀을 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 찹쌀의 사용량은 500g, 물의 사용량은 1.5L였으며 최종 제조된 농축액은 500mL, 분말은 350g이였다.Using glutinous rice, a solid medium was prepared in the same manner as in Example 1, inoculated and cultured with fingering mushroom hyphae to prepare a concentrate and a dry powder. At this time, the used amount of glutinous rice was 500g, the amount of water was used 1.5L, the final prepared concentrate was 500mL, the powder was 350g.
실시예 3: 보리를 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 3: Cultivation of Mushroom Mycelia and Production of the Products in Solid Medium Containing Barley
보리를 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 보리의 사용량은 500g, 물의 사용량은 1.5L였으며 최종 제조된 농축액은 550mL, 분말은 330g이였다.Using barley to prepare a solid medium in the same manner as in Example 1 and inoculated and incubated fingering mushroom mycelium to prepare a concentrate and dry powder. At this time, the amount of barley used was 500g, the amount of water used was 1.5L, the final prepared concentrate was 550mL, the powder was 330g.
실시예 4: 밀을 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 4 Culture of Mushroom Mycelia and Production of the Products in Solid Medium Containing Wheat
밀을 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 밀의 사용량은 500g, 물의 사용량은 1.5L였으며 최종 제조된 농축액은 600mL, 분말은 300g이였다.Using a mill to prepare a solid medium in the same manner as in Example 1, inoculated and incubated fingering mushroom mycelium to prepare a concentrate and dry powder. At this time, the amount of mill used was 500g, the amount of water was 1.5L, the final concentration of the prepared solution was 600mL, the powder was 300g.
실시예 5: 조를 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 5: Cultivation of Mushroom Mycelia and Production of the Products in a Solid Medium Containing Crude
조을 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 조의 사용량은 500g, 물의 사용량은 1.5L였으며 최종 제조된 농축액은 500mL, 분말은 350g이였다.Using a bath to prepare a solid medium in the same manner as in Example 1 and inoculated and incubated fingering mushroom mycelium to prepare a concentrate and dry powder. At this time, the amount of the bath used was 500g, the amount of water was 1.5L, the final prepared concentrate was 500mL, the powder was 350g.
실시예 6: 차수수를 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 6 Culture of Mushroom Mycelia and Production of the Products in Solid Medium Containing Water
차수수를 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 차수수의 사용량은 500g, 물의 사용량은 1.0L였으며 최종 제조된 농축액은 400mL, 분말은 350g이였다.A solid medium was prepared in the same manner as in Example 1 using the degree order, inoculated and inoculated with fingering mushroom mycelium to prepare a concentrate and a dry powder. At this time, the amount of used order was 500g, the amount of water was 1.0L, the final concentration of the prepared solution was 400mL, the powder was 350g.
실시예 7: 차기장을 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 7: Mushroom mycelium culture and production thereof in solid medium containing
차기장을 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 차기장의 사용량은 500g, 물의 사용량은 1.0L였으며 최종 제조된 농축액은 400mL, 분말은 350g이였다.A solid medium was prepared in the same manner as in Example 1 using a baejanggi, inoculated and inoculated with fingering mushroom mycelium to prepare a concentrate and a dry powder. At this time, the amount of used tea plant 500g, the amount of water was 1.0L, the final prepared concentrate was 400mL, the powder was 350g.
실시예 8: 율무를 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 8 Culture of Mushroom Mycelia and Production of the Products in Solid Medium Containing Yulmu
율무를 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 율무의 사용량은 500g, 물의 사용량은 1.5L였으며 최종 제조된 농축액은 500mL, 분말은 330g이였다.Using Yulmu, a solid medium was prepared in the same manner as in Example 1, inoculated and incubated with fingering mushroom mycelium to prepare a concentrate and a dry powder. At this time, the amount of used yulmu was 500g, the amount of water was 1.5L, the final concentration of the prepared solution was 500mL, the powder was 330g.
실시예 9: 팥을 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 9: Mushroom Mycelial Culture and Product Production in Solid Medium Containing Red Beans
팥을 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 팥의 사용량은 500g, 물의 사용량은 1.0L였으며 최종 제조된 농축액은 400mL, 분말은 350g이였다.Using a red bean, a solid medium was prepared in the same manner as in Example 1, inoculated and cultured with fingering mushroom mycelium to prepare a concentrate and a dry powder. At this time, the amount of used red beans was 500g, the amount of water was 1.0L, the final prepared concentrate was 400mL, the powder was 350g.
실시예 10: 옥수수를 함유하는 고체배지에서 버섯균사체 배양 및 그 산물 생산Example 10: Culture of Mushroom Mycelia and Production of the Products in Solid Medium Containing Corn
옥수수를 사용하여 상기 실시예 1과 동일하게 고체배지를 제조하고 운지버섯균사를 접종하고 배양하여 농축액과 건조분말을 제조하였다. 이때, 사용한 옥수수의 사용량은 500g, 물의 사용량은 1.0L였으며 최종 제조된 농축액은 400mL, 분말은 350g이였다.Using corn to prepare a solid medium in the same manner as in Example 1 and inoculated and incubated fingering mushroom mycelium to prepare a concentrate and dry powder. At this time, the amount of corn used was 500g, the amount of water was 1.0L, the final prepared concentrate was 400mL, the powder was 350g.
이상, 상기 실시예를 통하여 설명한 바와 같이, 본 발명은 곡물을 함유하는 고체배지에 식용 및 약용버섯 균사를 접종하고 배양하여 활착시킨 후 자가분해를 유도하여 항암성분 및 건강증진 물질이 함유된 자가분해물을 다량 생산하고 상기자가분해물은 기능성 건강식품 및 그 원료로 사용되는 뛰어난 효과가 있으므로 건강식품산업상 매우 유용한 발명인 것이다.As described above, the present invention, the present invention is inoculated with edible and medicinal mushroom mycelium in a solid medium containing grains and incubated after swelling to induce self-degradation to contain anti-cancer components and health promoting substances It is a very useful invention in the health food industry because it produces a large amount and the autolyzate has an excellent effect of being used as a functional health food and its raw materials.
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| KR1020000058805A KR20020027970A (en) | 2000-10-06 | 2000-10-06 | Process for mycelial culture using grain and product thereof |
| US09/971,004 US6569475B2 (en) | 2000-10-06 | 2001-10-05 | Process for mycelial culture using grain |
| JP2001309488A JP2002176968A (en) | 2000-10-06 | 2001-10-05 | Process for mycerial culture using grain, product therefrom and application of the product |
| CNB011427655A CN1171521C (en) | 2000-10-06 | 2001-10-08 | Method of culturing mycellium by using grain, its product use for culturing product |
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Cited By (12)
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| KR20010088514A (en) * | 2001-07-31 | 2001-09-28 | 장현유 | A cultivation medium composition of mushroom mycelium containing a roasted grain particle |
| KR20020085517A (en) * | 2001-05-09 | 2002-11-16 | 오종명 | The manufacture method of edible mushroom bacillus seed with grain |
| KR20030026140A (en) * | 2001-09-25 | 2003-03-31 | 김천환 | Mycellium production method of Cordyceps militaris, Inonotus obliquus and Phellinus linteus mushroom by use germinated grains and edible filber |
| KR20040018857A (en) * | 2002-08-27 | 2004-03-04 | 에코앤바이오 주식회사 | Cultural condition for the mycelial growth and the mycelium prepared therefrom |
| KR20040019583A (en) * | 2002-08-28 | 2004-03-06 | 류충현 | Mushroom cultivating process |
| KR100525298B1 (en) * | 2002-08-13 | 2005-11-02 | 송재만 | Process for grain containing extract of mycelial culture and healthy food using product thereof |
| KR100787170B1 (en) * | 2006-05-17 | 2007-12-21 | 이태봉 | Method of culture for inonotus obliquus using grain |
| KR101254503B1 (en) * | 2009-09-03 | 2013-04-19 | 동성제약주식회사 | Methods for manufacturing Mycelium of Inonotus Obliquss with Buckwheat media |
| KR20160056772A (en) | 2014-11-12 | 2016-05-20 | 박종례 | Method for production of fungi mycelia complex with Inonotus obliquus, Phellinus linteus and parassis Crispa |
| KR102183814B1 (en) * | 2020-05-08 | 2020-11-27 | (주)더마랩 | Preparation method of liquid culture of Lentinus edode mycelia and the liquid culture of Lentinus edode mycelia |
| CN114480142A (en) * | 2022-02-17 | 2022-05-13 | 河北国煦生物科技有限公司 | Mushroom liquid culture medium and matched bacterium culturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20020085517A (en) * | 2001-05-09 | 2002-11-16 | 오종명 | The manufacture method of edible mushroom bacillus seed with grain |
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| KR20030026140A (en) * | 2001-09-25 | 2003-03-31 | 김천환 | Mycellium production method of Cordyceps militaris, Inonotus obliquus and Phellinus linteus mushroom by use germinated grains and edible filber |
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| KR101254503B1 (en) * | 2009-09-03 | 2013-04-19 | 동성제약주식회사 | Methods for manufacturing Mycelium of Inonotus Obliquss with Buckwheat media |
| KR20160056772A (en) | 2014-11-12 | 2016-05-20 | 박종례 | Method for production of fungi mycelia complex with Inonotus obliquus, Phellinus linteus and parassis Crispa |
| KR102183814B1 (en) * | 2020-05-08 | 2020-11-27 | (주)더마랩 | Preparation method of liquid culture of Lentinus edode mycelia and the liquid culture of Lentinus edode mycelia |
| CN114480142A (en) * | 2022-02-17 | 2022-05-13 | 河北国煦生物科技有限公司 | Mushroom liquid culture medium and matched bacterium culturing method thereof |
| CN114480142B (en) * | 2022-02-17 | 2023-09-12 | 河北国煦生物科技有限公司 | Lentinus edodes liquid culture medium and matched fungus culturing method thereof |
| CN115572686A (en) * | 2022-10-10 | 2023-01-06 | 锡林郭勒职业学院 | Culture medium for producing Mongolian baili mushroom |
| CN115572686B (en) * | 2022-10-10 | 2023-12-12 | 锡林郭勒职业学院 | Culture medium for producing Mongolian white mushroom |
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