KR100189514B1 - The method for culturing mushroom hyphae - Google Patents

Abstract

1. TECHNICAL FIELD OF THE INVENTION

The present invention relates to a mass propagation method of mushroom mycelium using grain medium.

2. The technical problem to be solved by the invention

In the present invention, by using the grains that are readily available on the market for the cultivation of mushroom mycelium on the market, the production cost is reduced and the range of products used is not only pharmacological action, but also to enjoy the usual food, such as wheat flour. This widened the propensity to consume.

3. Summary of Solution of the Invention

The medium used for cultivating mushroom mycelium is cultured using grains (wheat, barley, brown rice, etc.), and the cultured grain medium is steamed for about 30 minutes using steam to keep harmful bacteria remaining. After sterilization and drying in a freeze dryer, using a grinder to grind into particles of about 120 mesh to complete the product.

4. Important uses of the invention

In the present invention, since the medium for cultivating mushroom mycelium is produced using grains, it is easy to secure the medium and pharmacological effect of the product, as well as abundant fiber, which is characteristic of grains, has a wide range of applications such as meal replacement. It has many advantages, such as

Description

Mass propagation method of mushroom mycelium using cereal medium

The present invention relates to a method for propagating mushroom mycelium, the mushroom mycelium in the method of cultivating mushroom mycelium of patent application No. 95-003045 described in the publication of Patent Application No. 94-024506 already disclosed by the inventor in the prior publication This is to increase the value as a commodity by expanding the application range after cultivation by using grain (edible grain) as a culture medium.

The culture medium used to culture the conventional mushroom mycelium was used as a commodity by culturing the mycelium of mushrooms using Korean ginseng or Ganoderma lucidum.

However, when using Korean ginseng or Ganoderma lucidum as a medium as described above, the expense of the medium itself is excessive, and accordingly, products produced by culturing mushroom mycelium were also expensive.

Then, the product produced by culturing the mushroom mycelium is produced by extracting the culture medium after the mushroom mycelium is cultured using a catalyst.

As a by-product is generated in the process of extracting the culture medium as described above may cause the problem of post-treatment, a large amount of medium is required to extract the high purity extract.

As such, when high-purity extract is extracted, the capsules are packaged by unit, and thus the supply cost of the product increases due to the increase in manufacturing cost, which has been a barrier to popularization of the product.

In addition, ginseng or ganoderma lucidum, which is a raw material of the medium, is a product having excellent pharmacological effect only by the raw material itself, so that the pharmacological effect is emphasized even though the medium produced by culturing mushroom mycelium is a health supplement or health food. There were disadvantages such that the range of consumption tendency could be reduced.

In the present invention, by using the grains that are readily available on the market for the cultivation of mushroom mycelium on the market, the production cost and the range of products used are not only pharmacological action, but also can be enjoyed in everyday life such as snacks such as wheat flour. In this way, the propensity to consume was widened.

In the present invention, after filling about two-thirds of the container in a transparent culture bottle made of 'polypropylene' resin capable of withstanding high temperature and high pressure of grain, which is a medium for culturing mushroom mycelium, the inlet of the container prevents drying during incubation and invasion of various bacteria. To prevent this problem, use a stopper with a breathable synthetic resin to seal it.

As described above, in order to sterilize the bacteria remaining in the medium and air in the container after the grains as raw materials of the medium, the container containing the medium is pressurized at a temperature of 110 ℃-120 ℃ in a high pressure sterilizer and heated for about 90 minutes. Sterilize.

The vessel heated as described above is transferred to a sterilized culture chamber maintained at an average of 18 ° C. and naturally cooled for about 12 hours.

After inoculating the mushroom hyphae on the sterilized medium as described above, in order to prevent the propagation of harmful microorganisms in the mushroom hyphae, the initial temperature is maintained at about 18 ° C. for 7-10 days and the mycelia of mushrooms are active. After confirming the state, the temperature is increased by 2-3 ° C. to perform the second growth culture.

After inoculating the mushroom mycelium on the medium as described above to give an appropriate environment for the culture of the mushroom mycelium in the sterilized culture chamber.

In the culture room, by sterilizing the ultraviolet light, indirect roughening method to emit light until the culture is completed to suppress the growth of bacteria harmful to the human body, and the control of moisture and temperature necessary for the cultivation is more accurate using an automatic forced circulation device To provide a stable environment.

In addition, the moisture of the medium required for the culture shows a difference depending on the individual characteristics of the mushroom mycelium, but can maintain about 60% on average, and the temperature is also constant around 18 ℃ -25 ℃ by the individual difference of the mushroom mycelium To be maintained. Then, for a smooth cultivation of mushroom mycelium during the culturing process, shaking the culture vessel after a certain time so that the medium is stirred, so that the mushroom mycelium can be evenly spread on the medium.

Grain medium cultured in the culture chamber as described above is steamed for about 30 minutes using steam to sterilize the remaining harmful bacteria, dried in a freeze dryer, and then crushed into particles of about 120 mesh using a grinder to complete the product. do.

The bulk growth method of the mushroom mycelium using the grain medium of the present invention as described above shows the difference in time and temperature of culturing according to the type of grain used as a medium, and thus the use of the product as a product changes according to the preferred embodiment of each grain medium. The following describes the and effects.

The following example describes the type of mushroom spawn on the basis of the spawn mushroom spawn.

Example 1

Pretreatment process for using wheat as a medium

The wheat is washed and first sterilized and dried until the moisture content in the dryer reaches an average of 60% to complete the pretreatment for incubation.

Feeding and Sterilizing Wheat Medium

After putting 450g-500g of wheat medium into the culture vessel (1000ml), prevent air permeability and drying of the medium, and maintain the airtightness of the culture vessel by using a stopper containing synthetic resin to prevent infiltration of general bacteria. Sterilize for 90 minutes at 120 ℃.

Cooling of the medium

The sterilized medium as described above is transferred to a culture chamber equipped with ultraviolet sterilization lamp, and then naturally cooled at an average temperature of 18 ° C. for at least 12 hours to lower the temperature of the culture medium in the culture vessel.

Mushroom hyphae input process

As described above, the culture vessel is set to an environment in which the mushroom spawn is easy to stick to the sterilized culture chamber to carry out phagocytosis. At this time, the inoculator wears a sanitized sanitary suit, sterilizes the inoculation rods and the spoon rods with alcohol lamps, opens the stopper of the culture vessel, and phages the medium in the culture vessel using the phagocytosis tool. Earn the entrance and seal it.

Culture process

When the culture is completed as described above, the culture vessel is transferred to the culture chamber to give the environment suitable for cultivation, and the appropriate temperature and humidity required for the cultivation of wheat medium are 22 ° C. and 60%, but propagation of various germs harmful to the human body at the beginning of the culture. In order to prevent the first 7 days of incubation to maintain the temperature of the culture chamber to about 18 ℃ and then incubate for 30 days at 22 ℃.

In order to smoothly spread the mushroom mycelium during the incubation period, the culture vessel is stirred by shaking the culture vessel artificially after a certain time.

As the culture proceeds, the mycelia are cultured in the culture vessel, and when the shiitake mushroom mycelium adheres, the wheat medium becomes white, and when the culture is completed, the culture vessel is filled with the white medium.

Sterilization and Product Manufacturing Process

The culture medium as described above is steamed for about 30 minutes using steam to sterilize the remaining various bacteria, and then dried in a freeze dryer, and ground to a particle size of about 120 mesh using a grinder, and then packaged in units. .

As described above, various kinds of medium are used to cultivate the mycelia of the mushroom, but when the grain is used, the produced product may increase the utilization according to the following medium, which will be described through other examples.

Example 2

When using barley as a medium, 500g of barley is subjected to a pretreatment process as in Example 1 after sterilization, and the barley medium is to be incubated for 35 days at an initial temperature of 18 ° C and 20 ° C thereafter. .

Example 3

In the case of using brown rice, 500 g of brown rice is subjected to a pretreatment process of one embodiment, and then the initial culture temperature is 18 ° C., and then 40 days at 25 ° C.

Example 4

In the case of using rice which is the most easy to obtain the type of grain in the grain medium, after the pretreatment process of Example 1, the initial culture temperature is 18 ℃ and then the temperature necessary for the cultivation is 25 ℃ to incubate for 50 days. do.

As described above, when the grains are used as a medium, the pharmacological action of the grains and the mushroom hyphae are complemented to each other to show various effects, and since the grains are used, it is easy to take them together with the wheat flour. It can be formed widely.

And it is natural that the mycelium of mushrooms can be cultured using grains not listed above.

The present invention as described above is because the medium for cultivating the mycelia of the mushroom is produced by using the grain, so as to secure the medium, as well as the pharmacological action of the efficacy of the product is rich in fiber, which is characteristic of the grain, the application range of products such as meal replacement Has a number of advantages, such as being broad.

Claims (1)

The medium used for culturing mushroom mycelia is cultured using grains (wheat, barley, brown rice, rice, etc.); In the culturing process, by indirect roughening the ultraviolet light sterilization in the culture vessel to emit light until the culture is completed to suppress the growth of bacteria harmful to the human body, and the control of the water and temperature necessary for the cultivation of an automatic forced circulation device To give a more accurate and stable environment; In addition, the moisture of the medium required for the culture shows a difference depending on the individual characteristics of the mushroom mycelium, but can maintain about 60% on average, and the temperature is also constant around 18 ℃ -25 ℃ by the individual difference of the mushroom mycelium To be maintained; Shaking the culture vessel after a predetermined time for smooth diffusion of the mushroom mycelium in the culture process so that the medium can be stirred; Grain medium cultured in the culture chamber as described above is steamed for about 30 minutes using steam to sterilize the remaining harmful bacteria, dried in a freeze dryer, and then crushed into particles of about 120 mesh using a grinder to complete the product. Mass propagation method of mushroom mycelium using a grain medium, characterized in that.