CN102816702B - Dazhu river mucor strain and method for rapid production of dazhu river red bean curd by using same - Google Patents
Dazhu river mucor strain and method for rapid production of dazhu river red bean curd by using same Download PDFInfo
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Abstract
The invention discloses a dazhu river mucor strain and a method for rapid production of dazhu river red bean curd by using the same. The method includes: soaking, washing, grinding beans, filtering pulp, cooking the pulp, coagulating the pulp, standing, squeezing and determining a blank, obtaining a bean curd white blank, inoculating spore suspension of dazhu river mucor according to 0.2-0.4% of the bean curd white blank, placing in a condition at the temperature of 25-30 DEG C and with 80-98% of humidity after the inoculating, performing 40-48h primary fermentation, cooling and rubbing after the fermentation, adding 11-13% of salt after the rubbing, 8-11% of alcohol, moderate chilli powder, ginger powder, garlic powder and other spices, subpackaging into small bottles at one time, vacuumizing, performing secondary fermentation for 40-45 days under the natural condition, and obtaining fermented bean curd when amino acid nitrogen content arrives at 0.42g/100g. The dazhu river mucor strain with high enzyme activity and strong heat resistance is adopted, the secondary fermentation time is shortened, and ripening time of the fermented bean curd is shortened under the precondition that the quality of the fermented bean curd is guaranteed.
Description
Technical field
The present invention relates to a kind of big bamboo river Mucor bacterial strain and the method for quick production big bamboo river red bean corruption thereof.
Background technology
Fermented bean curd (Fermented bean curd) claim again fermented bean curd, milkpea corruption, soy cheese, mold bean curd, bean curd with odor and the bean curd that becomes mildewed, and is one of the famous traditional fermentation bean product of China and seasonings.Fermented bean curd is the typical fermented type bean product of China and seasonings, flavour deliciousness, and unique flavor, nutritious, not only there is the nutritive value that soybean has, be also rich in biology, physiologically active substance, be easily absorbed by the body, there is stronger physiological hygiene function.Fermented bean curd, as the popular fermented bean products that the most effectively promotes soybean and tire, causes the even extensive concern of industry member of domestic and international human consumer and food, medical science day by day.
Production of preserved beancurd is with a long history, according to producing the bacterial classification difference using, can be divided into bacteria type, head mold type and Mucor type.Mucor has flora elongated flexible, can, by intact bean curd base surrounding, to keep the neat formalness of fermented bean curd finished product, now be widely used in Industrial processes.The enzyme system of Mucor is abundant, and protein hydrolysate is without bitter taste, and nontoxic, does not produce strange taste and heterochromatic etc.The quality of mucor strain directly has influence on output and the quality of fermented bean curd.The fermented bean curd Mucor of nature seed selection is subject to the restriction of climatic factor because heat-resisting ability is poor, proteinase activity, compared with low and can not guarantee protein appropriateness hydrolysis, has affected utilization, the turnover of fund and the Quality and yield of fermented bean curd of production of preserved beancurd producer factory building to a certain extent.Therefore, mucor strain is carried out to autotelic seed selection, therefrom filter out fast growth, spore quantity is many, enzyme activity is high, the bacterial strain of good heat resistance is significant.
At present, limit due to working condition and level, only limit to produce by the traditional way of spontaneous fermentation the winter that temperature is lower, quality product is extremely unstable.
Summary of the invention
The object of the invention is to solve the deficiencies in the prior art, for existing problem in traditional product suitability for industrialized production, sufu fermentation bacterial classification is vouched, separation screening, purifying, strain identification, the production of hybrid seeds and cultivation in early stage thereof, pickle, the production technique such as later stage fermentation, the gordian techniquies such as safety in production are studied, explore the mass-producing process production techniques of long-termization safety in production fermented bean curd, a kind of big bamboo river Mucor bacterial strain and the method for quick production big bamboo river red bean corruption thereof are provided, employing enzyme activity is high, the big bamboo river Mucor bacterial strain that thermotolerance is strong, shorten the secondary fermentation time, ensureing under the prerequisite of sufu quality, shorten fermented bean curd maturation time.
The object of the invention is to be achieved through the following technical solutions: a kind of big bamboo river Mucor bacterial strain, described big bamboo river Mucor belongs to the graceful bacterial classification of Mucor, this bacterial classification is in the center preservation of Chinese Typical Representative culture collection, Classification And Nomenclature is Actinomucor elegans SYY-M16, preserving number is CCTCC No:M2012179, and preservation date is on May 23rd, 2012; The colony characteristics of this bacterial classification is as described below: mycelia is compared with thick and grow, colony diameter 1～7cm, and height 13～15mm, bacterium colony quality is loose, and outward appearance is dried, opaque, presents white fine hair shape, and bacterium colony is tight with being connected of substratum, is difficult for picking;
Its bacterium colony individual morphology feature is as described below: white mycelium, have stolon and underdeveloped rhizoid, vegetative hyphae is infiltrated 1.5～2.0 mm in nutrient agar, and aerial hyphae extensive widespread stretches, when old and feeble, there is being chlamydospore avette, elliposoidal, aerial hyphae diameter 8～19 μ m; Sporangiophore diameter 12～20 μ m, proximal portion has branch, is single axle, and each branch and major branch top be raw sporocyst all; Described sporocyst is spherical in shape, and diameter is 63～91 μ m, and when tenderer, look shallow, and smooth surface, color depth when aging, and have coarse needle prick shape protuberate, last cracked spore and the cystospore of discharging of cyst wall, exposes columella; Described columella is avette, foreign plow-shape, smooth surface, and Individual Size differs greatly, and is (43～65) μ m × (24～46) μ m; Described sporangiospore is spherical in shape, diameter 7～10 μ m;
Described big bamboo river Mucor bacterial strain has following molecular biological characteristics: the sequence of big bamboo river Mucor bacterial strain 18S rDNA, and overall length 1652bp, its sequence is as follows:
GACTGTTGCC AGTCCTAACC TACTGATCCC ATATGCTCAC TATAACCATT CAATCGGTAG 60
TAGCGACGGG CGGTGTGTAC AAAGGGCAGG GACGTAATCA ACGCAAGCTG ATGACTTGCG 120
CTTACTAGGA ATTCCTCGTT GAAGAGCAAT AATTACAATG CTCTATCCCC AGCACGATGA 180
AGTTTCAAAA GTTTACCCAG ACCTTCCGGC CAAGGTTATA AACTCGCTGA CTTCATCAGT 240
GTAGCGCGCG TGCGGCCCAG AACATCTAAG GGCATCACAG ACCTGTTATT GCCTAAAACT 300
TCCTTCGGCT TGAAGCCGAT AGTCTCTCTA AGAAGCCAGA TAGATAAATC TATTCACCAA 360
CTAAAAAGTT GGCCTGCCTA TTTAGCAGAA TAAGGTCTCG TTCGTTATCG GAATTAACCA 420
GACAAATCAC TCCACGAACT AAGAACGGCC ATGCACCACC ACCCATAGAA TCTAGAAAGA 480
GCTTTCAATC TGTCAATCCT TACTATGTCT GGACCTGGTG AGTTTCCCCG TGTTGAGTCA 540
AATTAaGcCG CAGGCTCCAC TCCTGGTGGT GCCCTtCcGT CAATtCCtTT AAGTTTCAGC 600
ctTGCGTCCC ATACTCCCCC cAGAaCCCaA AAACTTTACT TTCGCTAAGA TGCTGAGCCA 660
GTCATAATAA ACAGGCCCAA TCTCTAGTCG GCATAGTTTG TGGTTAAGAC TACGACGGTA 720
TCTAATCGTC TTCGATcCCT TAACTTTAGA CCTTGATCAA TGAAAACGTC CTGGGTCAAA 780
TGCTTTCGCA GTAGTTTGTC TTCGGTCAAT CCAAGAATTT CACCTCTAGC GACCAAATAC 840
AAATGCCCCC AACCGTTTCT ATTCATCATT ACTCAAGTTC CTGAACCAAC GAAATAGGGA 900
CTAGAGTCAT ATTTCATTAT TCCATGCTAA CACATTCAAG CTTGAAAGCC TGCTTTAAAC 960
ACTCTGATTT GCTCATGGTA ATCATTTGGC TAGAGACTAT AGgCGACCGA AGCCACCCAT 1020
AGCATAACCA AATACAAAAG CCTCGGCAGA AGCGAGCTTG ATCAATGAAG ACCAGGCCAC 1080
CTCGCCTAAA GGCTAAAGTT TGACTACGGA CGTTTTAACT GCAACAACTT TAATATACGC 1140
TATTGGAGCT GGAATTACCG CGGCTGCTGG CACCAGACTT GCCCTCCAAT TGATCCTCGT 1200
TAAGGGATTT AAATTGTACT CATTCCAATT GCAAGACCTT AAAGGCCCTG CATTGTTATT 1260
TATTGTCACT ACCTCACCAT GTCGGTATTG GGTAATTTGC GCGCCTGCTG CCTTCCTTGG 1320
ATGTGGTAGC CGTTTCTCAG GCTCCCTCTC CGGAATCGAA CCCTAATTCC CCGTTACCCG 1380
TTGTAGGCAT TGTAAGCCTC TATCTTACAA TCTCAACCAT GATAGGGCAG AAAATCGAGT 1440
GGACCGTCGC CAGCACAAGG CCATGCGATC CGCTTAATTA TTATGAATCA CCAAGGGAAA 1500
CTGGTTTTAC CCAGCGTTGG CTTTATCTAA TAAGTGCACC CCTTCCGAAG TCAGGGCTTT 1560
TTTGCATGTA TTAGCTCTAG AATTACCACG GTTATCCAAG TAGTAAAGAA TATGTCACGT 1620
AGATCATAAC TGATTTAATG AGCCATTCGC AG 1652。
Big bamboo river Mucor bacterial strain is for producing fast a method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose a certain amount of soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.2～0.4% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 40～48h under 25～30 DEG C, the humidity condition that is 80～98% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 11～13, alcohol 8～11, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 40～45 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
The invention has the beneficial effects as follows:
(1) the invention provides a kind of big bamboo river Mucor bacterial strain and the method for quick production big bamboo river red bean corruption thereof, adopt the big bamboo river Mucor bacterial strain that enzyme activity is high, thermotolerance is strong, this bacterial strain is under 28～30 DEG C of conditions, riotous growth, its proteinase activity is high, can effectively shorten the fermented bean curd secondary fermentation time, and lower of normal temperature needs 40～45 days, fermented bean curd amino acid nitrogen content can reach 0.42g/100g, and fermented bean curd is ripe.Ensureing that under the prerequisite of sufu quality,, with respect to current 2～3 months producer's secondary fermentation time of production of preserved beancurd, fermented bean curd maturation time obviously shortens.To production of preserved beancurd producer rationally utilize factory building, promote fermented bean curd output, promote turnover of funds, to increase economic benefit significant;
(2) the invention provides a kind of big bamboo river Mucor bacterial strain and the method for quick production big bamboo river red bean corruption thereof, because bacterial strain enzyme activity provided by the invention is higher, the biochemical reactions such as proteolysis, mashing, organic acid fermentation, lipid generation carry out comparatively fast, add the spices cross reactions such as appropriate red chilly powder, Jiang Fen, garlic powder by traditional technology, the soft exquisiteness of red tofu product, the unique flavor that make;
(3) the invention provides a kind of big bamboo river Mucor bacterial strain and the method for quick production big bamboo river red bean corruption thereof, realize course of cultivating the microorganism automatization, a large amount of minimizing labor forces, raise labour efficiency, mechanization degree increases substantially, and can ensure better production sanitary condition, effectively control all physical and chemical indexs of preserved beancurd.Shorten the secondary fermentation time, improved the success ratio of soy cheese, reduce the loss of prior fermentation, shorten the production cycle.Be easy to large-scale production, make the producer be easy to hold and management, there is technique simple, ripe, be easy to grasp and control, be convenient to the advantages such as popularization.
Brief description of the drawings
Fig. 1 is schema of the present invention.
Embodiment
Below in conjunction with drawings and Examples, technical scheme of the present invention is described in further detail; but protection scope of the present invention is not limited to the following stated, some nonessential improvement that those skilled in the art's content according to the present invention is made the present invention and adjustment still belong to protection scope of the present invention.
Embodiment 1:
A kind of big bamboo river Mucor bacterial strain, described big bamboo river Mucor belongs to the graceful bacterial classification of Mucor, and this bacterial classification is in the center preservation of Chinese Typical Representative culture collection, and preserving number is CCTCC No:M2012179; The colony characteristics of this bacterial classification is as described below: mycelia is compared with thick and grow, colony diameter 1～7cm, and height 13～15mm, bacterium colony quality is loose, and outward appearance is dried, opaque, presents white fine hair shape, and bacterium colony is tight with being connected of substratum, is difficult for picking;
Its bacterium colony individual morphology feature is as described below: white mycelium, have stolon and underdeveloped rhizoid, vegetative hyphae is infiltrated 1.5～2.0 mm in nutrient agar, and aerial hyphae extensive widespread stretches, when old and feeble, there is being chlamydospore avette, elliposoidal, aerial hyphae diameter 8～19 μ m; Sporangiophore diameter 12～20 μ m, proximal portion has branch, is single axle, and each branch and major branch top be raw sporocyst all; Described sporocyst is spherical in shape, and diameter is 63～91 μ m, and when tenderer, look shallow, and smooth surface, color depth when aging, and have coarse needle prick shape protuberate, last cracked spore and the cystospore of discharging of cyst wall, exposes columella; Described columella is avette, foreign plow-shape, smooth surface, and Individual Size differs greatly, and is (43～65) μ m × (24～46) μ m; Described sporangiospore is spherical in shape, diameter 7～10 μ m;
Described big bamboo river Mucor bacterial strain has following molecular biological characteristics: the sequence of big bamboo river Mucor bacterial strain 18S rDNA, and overall length 1652bp, its sequence is as follows:
GACTGTTGCC AGTCCTAACC TACTGATCCC ATATGCTCAC TATAACCATT CAATCGGTAG 60
TAGCGACGGG CGGTGTGTAC AAAGGGCAGG GACGTAATCA ACGCAAGCTG ATGACTTGCG 120
CTTACTAGGA ATTCCTCGTT GAAGAGCAAT AATTACAATG CTCTATCCCC AGCACGATGA 180
AGTTTCAAAA GTTTACCCAG ACCTTCCGGC CAAGGTTATA AACTCGCTGA CTTCATCAGT 240
GTAGCGCGCG TGCGGCCCAG AACATCTAAG GGCATCACAG ACCTGTTATT GCCTAAAACT 300
TCCTTCGGCT TGAAGCCGAT AGTCTCTCTA AGAAGCCAGA TAGATAAATC TATTCACCAA 360
CTAAAAAGTT GGCCTGCCTA TTTAGCAGAA TAAGGTCTCG TTCGTTATCG GAATTAACCA 420
GACAAATCAC TCCACGAACT AAGAACGGCC ATGCACCACC ACCCATAGAA TCTAGAAAGA 480
GCTTTCAATC TGTCAATCCT TACTATGTCT GGACCTGGTG AGTTTCCCCG TGTTGAGTCA 540
AATTAaGcCG CAGGCTCCAC TCCTGGTGGT GCCCTtCcGT CAATtCCtTT AAGTTTCAGC 600
ctTGCGTCCC ATACTCCCCC cAGAaCCCaA AAACTTTACT TTCGCTAAGA TGCTGAGCCA 660
GTCATAATAA ACAGGCCCAA TCTCTAGTCG GCATAGTTTG TGGTTAAGAC TACGACGGTA 720
TCTAATCGTC TTCGATcCCT TAACTTTAGA CCTTGATCAA TGAAAACGTC CTGGGTCAAA 780
TGCTTTCGCA GTAGTTTGTC TTCGGTCAAT CCAAGAATTT CACCTCTAGC GACCAAATAC 840
AAATGCCCCC AACCGTTTCT ATTCATCATT ACTCAAGTTC CTGAACCAAC GAAATAGGGA 900
CTAGAGTCAT ATTTCATTAT TCCATGCTAA CACATTCAAG CTTGAAAGCC TGCTTTAAAC 960
ACTCTGATTT GCTCATGGTA ATCATTTGGC TAGAGACTAT AGgCGACCGA AGCCACCCAT 1020
AGCATAACCA AATACAAAAG CCTCGGCAGA AGCGAGCTTG ATCAATGAAG ACCAGGCCAC 1080
CTCGCCTAAA GGCTAAAGTT TGACTACGGA CGTTTTAACT GCAACAACTT TAATATACGC 1140
TATTGGAGCT GGAATTACCG CGGCTGCTGG CACCAGACTT GCCCTCCAAT TGATCCTCGT 1200
TAAGGGATTT AAATTGTACT CATTCCAATT GCAAGACCTT AAAGGCCCTG CATTGTTATT 1260
TATTGTCACT ACCTCACCAT GTCGGTATTG GGTAATTTGC GCGCCTGCTG CCTTCCTTGG 1320
ATGTGGTAGC CGTTTCTCAG GCTCCCTCTC CGGAATCGAA CCCTAATTCC CCGTTACCCG 1380
TTGTAGGCAT TGTAAGCCTC TATCTTACAA TCTCAACCAT GATAGGGCAG AAAATCGAGT 1440
GGACCGTCGC CAGCACAAGG CCATGCGATC CGCTTAATTA TTATGAATCA CCAAGGGAAA 1500
CTGGTTTTAC CCAGCGTTGG CTTTATCTAA TAAGTGCACC CCTTCCGAAG TCAGGGCTTT 1560
TTTGCATGTA TTAGCTCTAG AATTACCACG GTTATCCAAG TAGTAAAGAA TATGTCACGT 1620
AGATCATAAC TGATTTAATG AGCCATTCGC AG 1652。
Described big bamboo river Mucor bacterial strain is to separate Mucor bacterial strain from the rotten fermented bean curd of big bamboo river red bean of spontaneous fermentation, ultraviolet mutagenesis, and cultivate through temperature control, purifying obtains.Its feature is as follows:
(1) colony characteristics
At 28～30 DEG C, cultivate 72h, mycelia is compared with thick and grow colony diameter 1-7cm, highly 13～15mm.Bacterium colony quality is loose, and outward appearance is dry, opaque, presents white fine hair shape, and bacterium colony is tight with being connected of substratum, is difficult for picking.
(2) bacterium colony individual morphology
White mycelium, has stolon and underdeveloped rhizoid, and vegetative hyphae is infiltrated 1.5～2 .0mm in nutrient agar, and aerial hyphae extensive widespread stretches, and occurs being chlamydospore avette, elliposoidal, aerial hyphae diameter 8～19 μ m when old and feeble; Sporangiophore diameter 12～20 μ m, proximal portion has branch, is single axle, and each branch and major branch top be raw sporocyst all.Sporocyst is spherical in shape, and diameter is about 63～91 μ m, look shallow and smooth surface when tenderer, color depth have coarse needle prick shape protuberate when aging; Last cracked spore and the cystospore of discharging of cyst wall, exposes columella.Columella is avette, foreign plow-shape, smooth surface, Individual Size differs greatly, and is (43～65) μ m × (24～46) μ m.Sporangiospore is spherical in shape, diameter 7～10 μ m.
(3) physiological property: the temperature range that can better grow is 18～37 DEG C, high temperature resistant, at 28～30 DEG C mycelial growth plentiful dense, mycelia color and luster is pure white, its acid protease activity is 159.346u/g, neutral protease vigor is 188.215u/g, and basic protein enzyme activity is 121.462 u/g.
As shown in Figure 1, a kind of big bamboo river Mucor bacterial strain is for producing fast the method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose 10kg soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.3% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 40h under 28 DEG C, the humidity condition that is 80% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 12, alcohol 10, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 45 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
Moisture to fermented bean curd in fermenting process in the present invention, salt content, amino-acid nitrogen content, water-soluble protein content philosophy are according to GB 5009.3(aquametry), GB/T 12457-90(salt assay method), ZB X 66038 (amino nitrogen assay method), GB/T5009.5(protein determination) measure.
Embodiment 2:
The description of a kind of big bamboo river Mucor bacterial strain is with embodiment 1.
As shown in Figure 1, a kind of big bamboo river Mucor bacterial strain is for producing fast the method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose 10kg soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.2% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 48h under 30 DEG C, the humidity condition that is 90% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 13, alcohol 8, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 40 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
Moisture to fermented bean curd in fermenting process in the present invention, salt content, amino-acid nitrogen content, water-soluble protein content philosophy are according to GB 5009.3(aquametry), GB/T 12457-90(salt assay method), ZB X 66038 (amino nitrogen assay method), GB/T5009.5(protein determination) measure.
Embodiment 3:
The description of a kind of big bamboo river Mucor bacterial strain is with embodiment 1.
As shown in Figure 1, a kind of big bamboo river Mucor bacterial strain is for producing fast the method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose 10kg soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.4% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 45h under 25 DEG C, the humidity condition that is 95% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 11, alcohol 11, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 42 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
Moisture to fermented bean curd in fermenting process in the present invention, salt content, amino-acid nitrogen content, water-soluble protein content philosophy are according to GB 5009.3(aquametry), GB/T 12457-90(salt assay method), ZB X 66038 (amino nitrogen assay method), GB/T5009.5(protein determination) measure.
Embodiment 4:
The description of a kind of big bamboo river Mucor bacterial strain is with embodiment 1.
As shown in Figure 1, a kind of big bamboo river Mucor bacterial strain is for producing fast the method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose 10kg soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.3% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 48h under 28 DEG C, the humidity condition that is 98% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 11, alcohol 10, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 40 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
Moisture to fermented bean curd in fermenting process in the present invention, salt content, amino-acid nitrogen content, water-soluble protein content philosophy are according to GB 5009.3(aquametry), GB/T 12457-90(salt assay method), ZB X 66038 (amino nitrogen assay method), GB/T5009.5(protein determination) measure.
Embodiment 5:
The description of a kind of big bamboo river Mucor bacterial strain is with embodiment 1.
As shown in Figure 1, a kind of big bamboo river Mucor bacterial strain is for producing fast the method for big bamboo river red bean corruption, and it comprises the following steps:
S1: pre-treatment: choose 10kg soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.3% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 48h under 28 DEG C, the humidity condition that is 98% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 13, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 40 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
Moisture to fermented bean curd in fermenting process in the present invention, salt content, amino-acid nitrogen content, water-soluble protein content philosophy are according to GB 5009.3(aquametry), GB/T 12457-90(salt assay method), ZB X 66038 (amino nitrogen assay method), GB/T5009.5(protein determination) measure.
Sequence table
Municipal river, <110> Wanyuan food synthetic development corporation, Ltd.
<120> big bamboo river Mucor bacterial strain and for producing fast the method for big bamboo river red bean corruption
<160>1
<210>1
<211>1652
<212>DNA
<213> Mucor (Mucor)
<400>1
GACTGTTGCC AGTCCTAACC TACTGATCCC ATATGCTCAC TATAACCATT CAATCGGTAG 60
TAGCGACGGG CGGTGTGTAC AAAGGGCAGG GACGTAATCA ACGCAAGCTG ATGACTTGCG 120
CTTACTAGGA ATTCCTCGTT GAAGAGCAAT AATTACAATG CTCTATCCCC AGCACGATGA 180
AGTTTCAAAA GTTTACCCAG ACCTTCCGGC CAAGGTTATA AACTCGCTGA CTTCATCAGT 240
GTAGCGCGCG TGCGGCCCAG AACATCTAAG GGCATCACAG ACCTGTTATT GCCTAAAACT 300
TCCTTCGGCT TGAAGCCGAT AGTCTCTCTA AGAAGCCAGA TAGATAAATC TATTCACCAA 360
CTAAAAAGTT GGCCTGCCTA TTTAGCAGAA TAAGGTCTCG TTCGTTATCG GAATTAACCA 420
GACAAATCAC TCCACGAACT AAGAACGGCC ATGCACCACC ACCCATAGAA TCTAGAAAGA 480
GCTTTCAATC TGTCAATCCT TACTATGTCT GGACCTGGTG AGTTTCCCCG TGTTGAGTCA 540
AATTAaGcCG CAGGCTCCAC TCCTGGTGGT GCCCTtCcGT CAATtCCtTT AAGTTTCAGC 600
ctTGCGTCCC ATACTCCCCC cAGAaCCCaA AAACTTTACT TTCGCTAAGA TGCTGAGCCA 660
GTCATAATAA ACAGGCCCAA TCTCTAGTCG GCATAGTTTG TGGTTAAGAC TACGACGGTA 720
TCTAATCGTC TTCGATcCCT TAACTTTAGA CCTTGATCAA TGAAAACGTC CTGGGTCAAA 780
TGCTTTCGCA GTAGTTTGTC TTCGGTCAAT CCAAGAATTT CACCTCTAGC GACCAAATAC 840
AAATGCCCCC AACCGTTTCT ATTCATCATT ACTCAAGTTC CTGAACCAAC GAAATAGGGA 900
CTAGAGTCAT ATTTCATTAT TCCATGCTAA CACATTCAAG CTTGAAAGCC TGCTTTAAAC 960
ACTCTGATTT GCTCATGGTA ATCATTTGGC TAGAGACTAT AGgCGACCGA AGCCACCCAT 1020
AGCATAACCA AATACAAAAG CCTCGGCAGA AGCGAGCTTG ATCAATGAAG ACCAGGCCAC 1080
CTCGCCTAAA GGCTAAAGTT TGACTACGGA CGTTTTAACT GCAACAACTT TAATATACGC 1140
TATTGGAGCT GGAATTACCG CGGCTGCTGG CACCAGACTT GCCCTCCAAT TGATCCTCGT 1200
TAAGGGATTT AAATTGTACT CATTCCAATT GCAAGACCTT AAAGGCCCTG CATTGTTATT 1260
TATTGTCACT ACCTCACCAT GTCGGTATTG GGTAATTTGC GCGCCTGCTG CCTTCCTTGG 1320
ATGTGGTAGC CGTTTCTCAG GCTCCCTCTC CGGAATCGAA CCCTAATTCC CCGTTACCCG 1380
TTGTAGGCAT TGTAAGCCTC TATCTTACAA TCTCAACCAT GATAGGGCAG AAAATCGAGT 1440
GGACCGTCGC CAGCACAAGG CCATGCGATC CGCTTAATTA TTATGAATCA CCAAGGGAAA 1500
CTGGTTTTAC CCAGCGTTGG CTTTATCTAA TAAGTGCACC CCTTCCGAAG TCAGGGCTTT 1560
TTTGCATGTA TTAGCTCTAG AATTACCACG GTTATCCAAG TAGTAAAGAA TATGTCACGT 1620
AGATCATAAC TGATTTAATG AGCCATTCGC AG 1652
Claims (1)
1. a big bamboo river Mucor bacterial strain, is characterized in that: described big bamboo river Mucor belongs to the graceful bacterial classification of Mucor, and this bacterial classification is CCTCC No:M2012179 at the preserving number at Chinese Typical Representative culture collection center;
The colony characteristics of this bacterial classification is: at 28～30 DEG C, cultivate 72h, mycelia is compared with thick and grow, colony diameter 1～7cm, and height 13～15mm, bacterium colony quality is loose, and outward appearance is dried, opaque, presents white fine hair shape, and bacterium colony is tight with being connected of substratum, is difficult for picking;
The bacterium colony individual morphology feature of this bacterial classification is: white mycelium, have stolon and underdeveloped rhizoid, vegetative hyphae is infiltrated 1.5～2.0 mm in nutrient agar, and aerial hyphae extensive widespread stretches, when old and feeble, there is being chlamydospore avette, elliposoidal, aerial hyphae diameter 8～19 μ m; Sporangiophore diameter 12～20 μ m, proximal portion has branch, is single axle, and each branch and major branch top be raw sporocyst all; Described sporocyst is spherical in shape, and diameter is 63～91 μ m, and when tenderer, look shallow, and smooth surface, color depth when aging, and have coarse needle prick shape protuberate, and when cyst wall is cracked, discharge spore and cystospore, expose columella; Described columella is avette, foreign plow-shape, smooth surface, and Individual Size differs greatly, and is (43～65) μ m × (24～46) μ m; Described sporangiospore is spherical in shape, diameter 7～10 μ m;
This bacterial classification physiological property is: the temperature range that can better grow is 18～37 DEG C, high temperature resistant, at 28～30 DEG C mycelial growth plentiful dense, mycelia color and luster is pure white, its acid protease activity is 159.346u/g, neutral protease vigor is 188.215u/g, and basic protein enzyme activity is 121.462 u/g;
The molecular biological characteristics of described big bamboo river Mucor bacterial strain is: the sequence of big bamboo river Mucor bacterial strain 18S rDNA, and overall length 1652bp, its sequence is as follows:
GACTGTTGCC AGTCCTAACC TACTGATCCC ATATGCTCAC TATAACCATT CAATCGGTAG 60
TAGCGACGGG CGGTGTGTAC AAAGGGCAGG GACGTAATCA ACGCAAGCTG ATGACTTGCG 120
CTTACTAGGA ATTCCTCGTT GAAGAGCAAT AATTACAATG CTCTATCCCC AGCACGATGA 180
AGTTTCAAAA GTTTACCCAG ACCTTCCGGC CAAGGTTATA AACTCGCTGA CTTCATCAGT 240
GTAGCGCGCG TGCGGCCCAG AACATCTAAG GGCATCACAG ACCTGTTATT GCCTAAAACT 300
TCCTTCGGCT TGAAGCCGAT AGTCTCTCTA AGAAGCCAGA TAGATAAATC TATTCACCAA 360
CTAAAAAGTT GGCCTGCCTA TTTAGCAGAA TAAGGTCTCG TTCGTTATCG GAATTAACCA 420
GACAAATCAC TCCACGAACT AAGAACGGCC ATGCACCACC ACCCATAGAA TCTAGAAAGA 480
GCTTTCAATC TGTCAATCCT TACTATGTCT GGACCTGGTG AGTTTCCCCG TGTTGAGTCA 540
AATTAaGcCG CAGGCTCCAC TCCTGGTGGT GCCCTtCcGT CAATtCCtTT AAGTTTCAGC 600
ctTGCGTCCC ATACTCCCCC cAGAaCCCaA AAACTTTACT TTCGCTAAGA TGCTGAGCCA 660
GTCATAATAA ACAGGCCCAA TCTCTAGTCG GCATAGTTTG TGGTTAAGAC TACGACGGTA 720
TCTAATCGTC TTCGATcCCT TAACTTTAGA CCTTGATCAA TGAAAACGTC CTGGGTCAAA 780
TGCTTTCGCA GTAGTTTGTC TTCGGTCAAT CCAAGAATTT CACCTCTAGC GACCAAATAC 840
AAATGCCCCC AACCGTTTCT ATTCATCATT ACTCAAGTTC CTGAACCAAC GAAATAGGGA 900
CTAGAGTCAT ATTTCATTAT TCCATGCTAA CACATTCAAG CTTGAAAGCC TGCTTTAAAC 960
ACTCTGATTT GCTCATGGTA ATCATTTGGC TAGAGACTAT AGgCGACCGA AGCCACCCAT 1020
AGCATAACCA AATACAAAAG CCTCGGCAGA AGCGAGCTTG ATCAATGAAG ACCAGGCCAC 1080
CTCGCCTAAA GGCTAAAGTT TGACTACGGA CGTTTTAACT GCAACAACTT TAATATACGC 1140
TATTGGAGCT GGAATTACCG CGGCTGCTGG CACCAGACTT GCCCTCCAAT TGATCCTCGT 1200
TAAGGGATTT AAATTGTACT CATTCCAATT GCAAGACCTT AAAGGCCCTG CATTGTTATT 1260
TATTGTCACT ACCTCACCAT GTCGGTATTG GGTAATTTGC GCGCCTGCTG CCTTCCTTGG 1320
ATGTGGTAGC CGTTTCTCAG GCTCCCTCTC CGGAATCGAA CCCTAATTCC CCGTTACCCG 1380
TTGTAGGCAT TGTAAGCCTC TATCTTACAA TCTCAACCAT GATAGGGCAG AAAATCGAGT 1440
GGACCGTCGC CAGCACAAGG CCATGCGATC CGCTTAATTA TTATGAATCA CCAAGGGAAA 1500
CTGGTTTTAC CCAGCGTTGG CTTTATCTAA TAAGTGCACC CCTTCCGAAG TCAGGGCTTT 1560
TTTGCATGTA TTAGCTCTAG AATTACCACG GTTATCCAAG TAGTAAAGAA TATGTCACGT 1620
AGATCATAAC TGATTTAATG AGCCATTCGC AG 1652；
Described big bamboo river Mucor bacterial strain, for producing fast the method for big bamboo river red bean corruption, comprises the following steps:
S1: pre-treatment: choose a certain amount of soybean, soak the fixed soybean of elutriation, soak wash in a pan after soybean is carried out to milled processed, after grinding, filter out impurities, the filter pulp of gained is carried out to mashing off processing, after mashing off, carry out successively a slurry, crouching brain, squeezing, draw base processing, make bean curd openpore;
S2: inoculation: the spore suspension of inoculating big bamboo river Mucor by 0.2～0.4% of bean curd openpore on bean curd openpore;
S3: primary fermentation, cool flower, rub hair with the hands: it is primary fermentation 40～48h under 25～30 DEG C, the humidity condition that is 80～98% that inoculation is placed on temperature, carries out cool flower, rubs hair with the hands and process after fermentation;
S4: batching: the batching of adding following component and weight ratio: salt 11～13, alcohol 8～11, and add the appropriate at least spices including red chilly powder, Jiang Fen, garlic powder;
S5: packing, secondary fermentation: after having prepared burden, the disposable bottle that is distributed into, vacuumizes, under field conditions (factors), secondary fermentation 40～45 days, in the time that the amino acid nitrogen content recording reaches 0.42g/100g, fermented bean curd maturation, gets product.
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| CN112251361B (en) * | 2020-09-07 | 2022-07-29 | 广州市致美斋酱园有限公司 | Mucor strain and application thereof, mucor preparation, south milk and preparation method thereof |
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Non-Patent Citations (7)
| Title |
|---|
| 万源科技信息网.我市"耐高温毛霉菌腐乳菌种选育及其低盐无油产品开发与示范"项目通过省级科技成果鉴定.《万源科技信息网 网址：www.wyskjj.gov.cn/wywz/kjj/kjzxInfo.jsp?ID=541》.2012, |
| 万源科技信息网.我市"耐高温毛霉菌腐乳菌种选育及其低盐无油产品开发与示范"项目通过省级科技成果鉴定.《万源科技信息网 网址:www.wyskjj.gov.cn/wywz/kjj/kjzxInfo.jsp?ID=541》.2012, * |
| 李理等.毛霉蛋白霉在腐乳成熟中的作用.《食品科学》.2004,第25卷全文. |
| 李连华等.传统大豆制品的加工.《粮油加工工艺学》.成都科技大学出版社,1996,第112－116、120－121页. * |
| 杨立新等.雅致放射毛霉蛋白酶的液体发酵生产及应用研究.《优秀硕士学位论文全文数据库 工程科技I辑》.2005,(第3期),B024-7. |
| 毛霉蛋白霉在腐乳成熟中的作用;李理等;《食品科学》;20041231;第25卷;全文 * |
| 雅致放射毛霉蛋白酶的液体发酵生产及应用研究;杨立新等;《优秀硕士学位论文全文数据库 工程科技I辑》;20050715(第3期);第1-5页，第二、四章节 * |
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