KR100368694B1 - Laver Containing Mycellium of Mushroom and Preparing Method thereof - Google Patents

Abstract

The present invention relates to a laver containing mushroom mycelium and a method for producing the same, and more particularly, to a method for producing laver cultured mycelium by using an aid as a medium and a laver produced by the method. According to the present invention, in addition to the inherent taste and nutrition of laver, it is possible to produce laver with the nutrition, taste and aroma of mushrooms.

Description

Laver Containing Mycellium of Mushroom and Preparing Method

The present invention relates to a laver containing mushroom mycelium and a method for producing the same, and more particularly, to a method for processing laver cultured mycelium using an aid as a medium and a laver produced by the method.

Mushrooms are botanical microorganisms belonging to basidiomycetes or asymptomatic fungi, and over 15,000 mushrooms are grown worldwide, and about 50% of them are estimated to be edible. In Korea, about 230 kinds of edible or medicinal mushrooms have been reported. Among the mushrooms that are widely used for edible and medicinal mushrooms, the mushrooms that are artificially cultivated include shiitake ( Lentinula edodes ), pleurotus spp. And Flammulina velutipes. ), Ganoderma lucidum , Agaricus spp. And Paecilomyces japonica .

In order to artificially cultivate mushrooms, spawns, which are secondary mycelia of seeds, are used as seeds. The spawn used in mushroom cultivation should be easily decomposed by mushroom mycelium, so that the mycelial growth rate is fast and its adaptability to cultivation medium should be excellent. Most of the spawns currently in use were developed at the early stages of artificial cultivation, using solid media such as wood (stock), sawdust, rice straw, waste noodles, cotton husks (crustacean shells) and their mixed media. For example, raw wood (stock) spawns are removed from wood where mushroom mycelia are widespread and plugged into logs. As a spawn commonly used, various nutrients are added to sawdust and used as mushroom spawn.

In recent years, with the rapid development of biotechnology, many mushrooms have attracted attention as materials for functional food and drug development. Edible mushrooms are evaluated by the quality and content of the components involved in nutritional characteristics (primary function), preference characteristics (secondary function), and dynamic bioregulatory function (tertiary function), which are static characteristics before eating. do. That is, mushrooms are nutritious (primary function); Tasty, visually beautiful, and not only contribute greatly to appetite by the unique aroma (secondary function); It has been found to contain various effective ingredients that exhibit pharmacological effects such as antibacterial action, antiviral action, cardiovascular action, cholesterol lowering action, blood sugar strengthening action, blood pressure lowering action, antithrombotic action and anti-tumor action. function).

On the other hand, laver ( Porphyra tenera ) is a representative cultured red algae, and there are large sesame, sesame ( P. Tenera ) and radiation laver ( P. Yezoensis ). The quality of laver varies according to the time of production, and it is ideal to harvest it in mid-January, which contains the most nucleic acids. Until now, the processing of laver has generally been a simple processing process such as harvesting, drying and seasoning.

The inventors noticed that 30% of protein and 40% of carbohydrates are present in the seaweed components, and by cultivating the mushroom mycelium using the aid before the processing of seaweed as a medium, To prepare the present invention was completed.

It is an object of the present invention to provide a laver added with functions such as nutrition, taste and aroma of mushrooms and a method for producing the same.

In order to achieve the above object, in the present invention, by using the aid as a medium and adding a carbon source such as grain flour to cultivate the mushroom mycelium, in addition to the intrinsic taste and nutrition of seaweed, a method of producing a laver with the nutrition, taste and flavor of the mushroom to provide.

Hereinafter, the present invention will be described in detail.

The present invention provides a laver containing mushroom mycelium and a method for producing the same.

That is, the present invention provides a laver cultured mycelium using the aid as a medium, this laver is prepared by culturing the mushroom mycelium with the aid as the main raw material and the carbon source as a secondary raw material, the manufacturing process is as follows Steps include:

(a) combining aid, carbon source and water to sterilize at a temperature of 100 to 300 ° C;

(b) sterile inoculation of the mushroom spawn in the sterilized aid and incubating at a temperature of 20 to 30 ℃; And

(c) processing the original growth of the mushroom mycelium.

Examples of aids there is after harvesting aid used in step (a), the cleaning to remove dirt, sand, japtae and soda, and blending was dehydrated with a dehydrator of three appropriate state to the compressor, may be used pyropia tenera (P Tenera ) or radiation-patterned laver ( P. Yezoensis ).

In addition, the culturing step may be performed for 5 to 15 days in an air floating bioreactor or a rotating drum bioreactor, it is preferable to use an air floating bioreactor. At this time, the culturing step can be performed using a conventional incubator, of course.

The use of rotary drum bioreactors is much less than the use of air-rich bioreactors. That is, when cultivated using an air rich bioreactor, the aid is used in an amount of 10 to 15% of the weight of water used, and the carbon source is used in an amount of 1 to 5% so that the amount of water used is aid and Much more than the amount of carbon. On the other hand, when cultured using a rotary drum type bioreactor, it can be seen that the amount of water used is 60 to 70% of the total weight of the aid and the carbon source, which is very small. This can be understood in view of the fact that rotary drum bioreactors are mainly used for the cultivation of solid components.

Since the amount of moisture varies depending on the reactor used, the processing method is inevitably different. In other words, when cultured using an air-floating bioreactor, a rotating drum-type bioreactor is performed while a process of drying and seasoning the raw material is carried out using a claw (kimbal) because the water content of the cultured aid is high. Unlike the cultivated in the air floating bioreactor, the cultivated in is almost no moisture, so when the cultivation is completed, a separate drying process using a foot is not necessary. It can be used as a processed food.

The carbon source used in the manufacturing process may be used as long as it can provide carbon, and all grains of powder or glucose may be used.

Also, the mushroom spawn that can be used can be used as spawn of all edible mushrooms, especially Agaricus brazei , Phellinus linteus , Cordyeeps Syensis , Shiitake mushroom ( Lintinula edodes ), Pleurotus spp. ., Flammulina velutipes , Ganoderma lucidum , Mushroom Mushroom ( Agaricus spp.), Matsutake Mushroom ( Tricholoma matsutake ), Mushroom ( Auricularia auricula ), Wolfiporia cocos , Hypodzigus It is preferable to use strains of mushrooms selected from the group consisting of marmoreus ), Grifola frodosa and Armillaria spp ..

Hereinafter, the present invention will be described in detail by examples. However, the examples are only to illustrate the invention and the present invention is not limited by the following examples.

[Production example] Collection and processing of aid

The collected aid was washed well in seawater to remove mud, sand, and blemishes, and then washed two to three times in fresh water for drinking to remove salt. The washed original was dewatered with a dehydrator and then rinsed with a compressor.

Example 1 Preparation of Agaricus Laver (1)

10 kg of P. Tenera aid and 4 kg of glucose were washed and mixed with 100 L of drinking water in the air preparation bioreactor and sterilized at 121 ° C. for 1.5 hours. Then 10 liters of Agaricus brazei strains were sterilely inoculated and incubated at 25-28 ° C. for 7 days.

The cultured aid was poured into a collecting tank, and hot air drying was performed using a foot pad. The color of laver with 3% moisture content was pale yellow due to mycelium, but after it was baked for 30 seconds at 180 ℃ with sesame oil and salt, it turned blue. Agaricus laver was prepared by cutting the baked laver into a rectangular shape having a size of 5 cm × 8 cm.

Example 2. Preparation of Situation Seaweed (1)

Soy flour 2 kg was dissolved in 5 L of drinking water, heated to 100 ° C and mixed. This was washed by the method of Preparation Example and mixed with 10 kg of fine P. Yezoensis aid and put into an air culture bioreactor with 100 L of drinking water and sterilized for 1.5 hours at 121 ℃. Then 10 liters of Phellinus linteus spawn were sterilely inoculated and incubated at 25 to 28 ° C. for 10 days.

This cultured aid was processed in the same manner as in Example 1 to prepare a laver.

Example 3 Preparation of Cordyceps Sinensis (1)

2 kg of flour was dissolved in 5 L of drinking water, heated to 100 ° C, and mixed. 100 g of silk peptide powder was added to the mixture, which was then washed, and then mixed with 10 kg of fine P. Tenera aid and prepared in an air-rich bioreactor with 100 L of drinking water. Put and sterilized at 121 ℃ for 1.5 hours. Then 10 L of Cordyeeps syensis spawns were sterilely inoculated and incubated at 25-28 ° C. for 6 days.

This cultured aid was processed in the same manner as in Example 1 to prepare a laver.

Example 4 Preparation of Agaricus Laver (2)

10 kg of P. Tenera aid and 4 kg of glucose were washed and mixed with 9 L of drinking water and sterilized at 121 ° C. for 1.5 hours. Then 10 liters of Agaricus brazei strains were sterilely inoculated and incubated at 25-28 ° C. for 7 days.

Agaricus laver was prepared by seasoning the raw laver in the finished culture.

Example 5 Preparation of Situation Seaweed (2)

Soy flour 2 kg was dissolved in 2 L of drinking water, heated to 100 ° C and mixed. This was washed by the method of the preparation example and mixed with 10 kg of fine P. Yezoensis aid and put into a rotary drum type bioreactor with 8 L of drinking water and sterilized for 1.5 hours at 121 ℃. Then 10 liters of Phellinus linteus spawn were sterilely inoculated and incubated at 25 to 28 ° C. for 10 days.

This cultured aid was processed in the same manner as in Example 4 to prepare a laver.

Example 6 Preparation of Cordyceps Sinensis (2)

2 kg of flour was dissolved in 2 L of drinking water, heated to 100 ° C, and mixed. 100 g of silk peptide powder was added to the mixture, which was then washed, and then mixed with 10 kg of fine P. Tenera aid and prepared in a rotary drum bioreactor with 8 L of drinking water. Sterilized for time. Then 10 L of Cordyeeps syensis spawns were sterilely inoculated and incubated at 25-28 ° C. for 6 days.

This cultured aid was processed in the same manner as in Example 1 to prepare a laver.

As described above, according to the present invention by cultivating the mycelium of mushrooms, which has recently attracted attention as a functional food and drug development material by using the aid as a medium, in addition to the unique taste and nutrition of seaweed, A laver with a flavor can be easily produced.

Claims (12)

delete (a) Take P. Tenera or P. Yezoensis Aid and wash it to remove mud, sand, blemishes and salts, dewater with a dehydrator and rinse with a compressor. To this, a step of sterilizing at a temperature of 100 to 300 ℃ by combining a carbon source and water selected from glucose, soy flour and flour; (b) aseptically inoculate the mushroom spawn to the sterilized aid, and incubated in an air culture bioreactor or a rotary drum bioreactor at a temperature in the range of 20 to 30 ° C., but incubated using the air culture bioreactor, The aid is used in an amount of 10 to 15% of the weight of water, the carbon source is used in an amount of 1 to 5% of the weight of water, and when cultivated using a rotary drum bioreactor, the amount of water used is the amount of the total weight of the aid and carbon source. Culturing for 5 to 15 days at 60 to 70%; And (c) processing the aid in which the mushroom mycelium is grown, but if culturing using an air floating bioreactor, the aid is dried using a paw and then seasoned, and in the case of culturing using a rotary drum type bioreactor, the bioreactor Method of producing laver containing mushroom mycelium, characterized in that it comprises the step of seasoning immediately laver in culture. delete delete delete delete delete delete delete delete The method of claim 2, The mushroom spawn is Agaricus brazei , Phellinus linteus , Cordyceeps Syensis , Lentinula edodes , Pleurotus spp., Flammulina velutipes , Ganoderma lucidum ( Ganoderma) lucidum), matsutake mushroom (Tricholoma matsutake), mushroom (Agaricus spp.), fungus (Auricularia auricula), Poria cocos (Wolfiporia cocos), only strands mushrooms (Hypsizigus marmoreus), Maitake mushrooms (Grifola frodosa) and mulberry tree mushrooms (Armillaria spp.) A method for producing laver containing mushroom mycelium, characterized in that the strain of the mushroom selected from the group consisting of. delete