KR20160053173A - Composition for anti-aging comprising extract of Boehmeria nivea (L.) GAUDICH Root - Google Patents

Abstract

The present invention relates to a cosmetic composition for inhibiting the skin wrinkle formation and improving the skin, which comprises the extract of Momordiae radix as an active ingredient.
According to the present invention, the root extract of the present invention, which is a specific region of the ramie root, promotes the biosynthesis of collagen, which is the main component of the skin dermis, and inhibits the amount of MMPs that accelerate the decomposition of dermis, can do.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for inhibiting and improving skin wrinkle formation, which comprises an extract of Panax ginseng CA Meyer as an active ingredient (Composition for anti-aging comprising Boehmeria nivea (L.) GAUDICH Root}

More particularly, the present invention relates to a cosmetic composition for inhibiting and improving skin wrinkle formation, which comprises an extract of Panax ginseng as an active ingredient.

Skin aging is manifested by wrinkles, decreased elasticity, and pigmentation. The most prominent phenomenon of aging is wrinkles. The causes of aging of the skin that cause such wrinkles can be roughly classified into two types. It is the internal factor which is the natural aging according to the age and the external factor which is caused by the external environment. Exogenous factors include ultraviolet rays, pollution, disease, drinking and smoking. Inner factors include free radicals and inflammation, stress, decreased cell activity and mutation, and cell metabolic imbalance (Physiology Of The Skin, Allured Publishing Corporation, Peter T. Pugliese. MD, p. 61-72). The cause of wrinkles according to the structure of the skin is described in more detail. The skin is divided into epidermis and dermis. The cause of wrinkles in the epidermis is the accumulation of stratum corneum due to the decrease of turn-over of keratinocytes, thickening of stratum corneum, decrease of water content ability of stratum corneum, and wrinkling of keratin. The boundary between epidermis and dermis is involved in epidermal cell support, adhesion, migration of nutrients, and control of epidermal differentiation. Collagen IV and VII are decreased due to various aging factors, especially UV, , The selective permeation function is weakened, and harmful components easily affect the dermis, resulting in wrinkles (Keene, J. Cell Biol., 104, p611-621, 1987). The dermis is composed of the connective tissue below the epidermis. It is not densely packed like keratinocyte as epidermal cell, but it is filled with extracellular space and macromolecular network called extracellular matrix. This extracellular epilepsy is made up of fibroblasts in the dermis and consists of fibrous proteins such as collagen and elastin and polysaccharides called acidic mucopolysaccharides such as hyaluronic acid. Extracellular epilepsy is directly involved in skin elasticity, metabolism, and vitality. More specifically, an acidic mucopolysaccharide maintains a large amount of water in its molecule to exhibit a gel phase, and the water in the gel is responsible for penetrating nutrients, metabolites, and hormones from blood vessels to cells in tissues . Therefore, if the production of the acidic mucopolysaccharide in the extracellular epilepsy is promoted, not only the skin becomes crispy but also the metabolism of the skin is promoted, thereby making it possible to prevent aging of the skin. In addition, fibrous proteins are responsible for maintaining tissue morphology and imparting elasticity to the skin. Therefore, when the production of fibrous protein in extracellular epilepsy is promoted, it gives elasticity to skin, and wrinkles or fine lines are suppressed. When the activity of fibroblasts producing such extracellular epilepsy is lowered by internal or external factors of aging, especially UV, collagen biosynthesis is decreased and denatured elastin is increased. In addition, the increase of matrix metalloproteinases (MMPs), which break down the extracellular epilepsy, increases the degradation of epilepsy such as collagen, the formation of solar scars in the dermis, the destruction of the epidermis - And the dermal decomposition is accelerated. As a result, the elasticity, freshness, and vigor of the skin are lost, and wrinkles, fine lines and roughness of the skin are generated, which is one cause of skin aging (Funtional Cosmetology, Society of Cosmetic Chemists of Japan, pp. 163-180; Et al., Journal of Investigative Dermatology, V.107, N.3, 1996; Brenneisen P. et.al., Photochemistry & Photobiology, V.64, N.5, 1996). In particular, collagen I is the major component of the skin dermis and is degraded by MMP-1. Attempts have been made to find a method for maintaining young skin that is alive by increasing the amount of synthesis of extracellular epilepsy, such as collagen, which accounts for the largest portion of the wrinkle formation factors, and inhibiting MMP-1.

In recent years, it has been widely known that substances having an absorption action, extracted and separated from natural materials, usually have less irritation to the skin and have other pharmacological actions such as astringent action and antibacterial action. Therefore, Are being studied.

As a part of the above-mentioned attempt, the root of the specific part of the natural product (Boehmeria nivea (L.) GAUDICH) studied by the present inventors belongs to the nettle family. It is a perennial herbaceous plant. Its origin is in Korea and it is distributed in the southernmost part of the country. It lives in a humid and warm region. It is 1 ~ 2m in height. Its stem is round and green, its branches are split, and there are many fine hairs spread with petiole. Flowers bloom in July ~ August, and male and female are hanging on the axilla, shorter than petiole, flower on the lower part of the female flower, on the upper part of the stem, 5 ~ 10cm long. The male flower has 4 hairs and 4 stamens. The female flower is wrapped in a tubular horn and consists of 1 pistil. Fruits are oval, oval shaped, several attached together and 1mm long. The roots are irregularly circumferential, slightly curved, grayish brown on the outside, very rough, with vertical wrinkles and long horizontal shrubs. There are protruding fine roots and many small horns. The peeled part is grayish brown to yellowish brown, and the quality is hard, brittle and light. The cross-section is fibrous, a little powdery, fresh roots to the inside, and sometimes with several ring-like patterns. Ingredients contain phenols, tryptophanoid (or sterol), and chlorogenic acid, and outposts and seeds contain hydrocyanic acid. Moxifloxacin is used to treat hemorrhagic, hematochezia, nosebleeds, urine bleeding, uterine bleeding, intestinal hemorrhage, intestinal hemorrhage, abdominal pain, sudden onset anxiety, and cystitis, rash caused by open fever, and snakebite symptoms. Korean Patent Laid-Open No. 10-2003-0074978 discloses a composition for inhibiting obesity of a late stage extract, and Korean Patent No. 10-0489564 describes a composition for reducing blood sugar containing a late stage extract, The present invention does not disclose a cosmetic composition for inhibiting the skin wrinkle formation and improvement of the extract of Panax notoginseng. In addition to this, research on moxifloxacin has been reported to reduce bleeding, shorten bleeding time, and antimicrobial activity. In addition, the extract of Mossy muscle ethanol of the present invention showed an effect of inhibiting the expression of MMP-1, which is superior to the ethanol extract of the cosmetic composition for inhibiting skin wrinkle formation and containing the extract of Mossy mussel, which has not yet been disclosed.

Accordingly, the inventors of the present invention searched for a substance effective for inhibiting and improving skin wrinkle formation, and found out substances that are effective for preventing skin wrinkling among various natural substances of nature. As a result, it was found that the ingredient extracted from the root Exhibit very excellent skin wrinkle formation inhibiting and improving effects, and the present invention has been completed.

KR 10-2003-0074978 A KR 10-0489564 B

Accordingly, it is a main object of the present invention to provide a cosmetic composition for inhibiting and improving skin wrinkle formation containing an extract of a hairy root having an effect of inhibiting and improving skin wrinkle formation as an active ingredient.

According to one aspect of the present invention, there is provided a cosmetic composition for suppressing and improving wrinkle formation, which comprises an extract of Momomoe root as an active ingredient.

The present inventors screened substances effective for preventing wrinkles among various natural plants in order to search for a method of suppressing and improving wrinkle formation by controlling collagen and MMP-1, which are the most important factors of wrinkle formation, It was confirmed that the component extracted from the specific root, roots (waxy root) exhibits a very excellent anti-wrinkle effect, and the present invention has been completed.

In the present invention, the extract may be extracted with any solvent conventionally used, and is preferably extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof .

The above extract can be obtained through the following steps. Specifically, the dried muscle is selected from the group consisting of water containing purified water of 2 to 10 times, preferably 5 to 10 times its volume, lower alcohol of 1 to 4 carbon atoms, butylene glycol, propylene glycol and glycerin At least one solvent; An extraction temperature of 20 to 100 DEG C, preferably 20 to 60 DEG C in a state where a cooling condenser is installed to prevent the solvent from evaporating; 1 to 30 hours, preferably 3 to 20 hours; 1 to 7 times, preferably 1 to 3 times; Extraction using cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heat extraction, preferably hot water extraction; The obtained extract is filtered, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dried extract.

In the present invention, the extract may be contained in an amount of 0.01 to 20% by weight, preferably 0.1 to 10% by weight, based on the total composition. Outside of the above range, the effect is insignificant.

In the present invention, the composition is characterized by exhibiting an effect of inhibiting and improving wrinkle formation by inhibiting the expression of collagenase (MMP-1).

According to the experimental example of the present invention, the amount of MMP-1 expressed in the group treated with the extract of the hairy root of the present invention was smaller than that of the group treated with the extract of the present invention. . The inhibition of MMP-1 expression was observed especially in the ethanol extract of Ramis muscle. From these results, it can be concluded that the extract of Panax ginseng extract of the present invention inhibits the expression of MMP-1 promoting collagen degradation, thereby effectively suppressing and improving the skin wrinkle formation (Experimental Example 2).

In the present invention, the composition is characterized by exhibiting an effect of suppressing and improving wrinkle formation by promoting collagen synthesis.

According to the experimental example of the present invention, it was confirmed that the hairy root extract of the present invention increased the amount of type I collagen expression, and in particular, the hairy root extract showed excellent MMP-1 expression inhibition. These results indicate that collagen, one of the fibrous proteins that impart skin elasticity and maintain tissue morphology, maintains skin elasticity, thereby suppressing wrinkles and fine lines, thereby effectively suppressing and improving skin wrinkle formation Example 3).

In the present invention, the composition may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizer cream, a hand cream, , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

Hereinafter, the present invention will be described more specifically.

The extract of the present invention can be used variously in cosmetics and facial cleansers having an effect of suppressing and improving wrinkles of the skin. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, etc., and cleansing, cleansing agents, soaps, treatments, and essences

The cosmetic composition of the present invention may further comprise a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability The vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may contain, in addition to the above-mentioned essential components, other components usually added to the cosmetic composition.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Propyl isostearate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight, And preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics. Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The hairy root extract of the present invention can inhibit and improve the skin wrinkles by promoting the biosynthesis of collagen, which is the main component of the skin dermis, and inhibiting the amount of MMPs synthesized to accelerate the dermal degradation.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  One: Moshi  60 ° C Purified water  Extract preparation

The dried rhizome root (300 g) was poured into 2.4 Kg of purified water, connected with a reflux condenser, extracted with hot water at 60 ° C for 24 hours, filtered through 2500 mesh filter cloth, and filtered through a 0.45 μm filter. Thereafter, the solvent was completely removed from the filtrate by using a rotary evaporator under reduced pressure to obtain 15.3 g of powdery mouton root extract powder.

Example  2: Moshi  30% EtOH  Extract preparation

300 g of the dried muscle was put into 3 kg of 30% ethanol, connected with a reflux condenser, extracted with hot water at 50 ° C for 24 hours, filtered through 2500 mesh filter cloth, and filtered through a 0.45 μm filter. Thereafter, the solvent was completely removed from the filtrate by using a rotary evaporator under reduced pressure to obtain 14.1 g of powdery mouton root extract powder.

Example  3: Moshi  50% EtOH  Extract preparation

11.2 g of the muscle root extract powder was obtained by the method of Example 2 above.

Example  4: Moshi  70% EtOH  Extract preparation

5.7 g of a muscle root extract powder was obtained by the method of Example 2 above.

Experimental Example  1: Cytotoxicity measurement

The cytotoxicity of monocyte cell culture was measured for MTT assay (MTT assay, J. Immunological Methods, 65, p55, 1983). MTT assay is an experimental method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are soluble in mitochondria and have a yellow salt, MTT (3- [4,5-dimethlythiazole-2-yl] -diphenyl tetrazolium bromide) is reduced to a blue formazan derivative which is insoluble in water by succinate dehydrogenase or mitochondrial dehydrogenase. The resulting formazan derivative is dissolved in a solvent (usually using dimethlysulfoxide) and the absorbance is measured. More specifically, human normal fibroblasts (ATCC CRL-2076) were inoculated on a 24-well plate at a concentration of 1 x 10 < ^{5 &} gt ;. The medium is replaced with IMDM (Iscove's modified Dulbecco's medium, Gibco, USA) containing 10% bovine serum, and the test sample is added for each concentration and cultured for 1 day. After that, the medium was removed and 1 ml of 0.25 mg / ml MTT solution was treated and reacted at 37 ° C for 4 hours. Then, 1 ml of DMSO (dimethylsulfoxide) was added to the cells from which the MTT solution had been removed to dissolve the MTT formazan, and the absorbance at 570 nm was measured. The measurement results are shown in Table 1.

Test group Experimental concentration (μg / ml) Cell survival rate (%) Example 1 One 101.05 10 99.76 Example 2 One 100.04 10 99.63 Example 3 One 100.36 10 99.08 Example 4 One 101.26 10 100.54

As a result, as shown in Table 1, it was found that the cell survival rate of the hairy root extracts was close to 100%, indicating no cytotoxicity.

Experimental Example  2: MMP -One mRNA  Measurement of expression inhibition amount

The MMP-1 mRNA inhibitory effect was measured on the moxa root extract. To measure the expression level of the MMP-1 gene, Applied Biosystems 7500 FAST Real-Time PCR System (Applied Biosystems, CA, USA) was performed. Human normal fibroblasts (ATCC CRL-2076) were grown in IMDM medium (Gibco, USA) containing 10% bovine serum (Gibco, USA) at a density of 1 × 10 ⁶ in a 60 mm dish at 37 ° C. in a 5% CO ₂ incubator And cultured for one day. Subsequently, the medium is washed twice with HBSS (Hank's Balanced Salt Solution), then irradiated with UVA 6 J / cm ² , replaced with IMDM containing no bovine serum, added with the sample, and cultured at 37 ° C. for 24 hours. After incubation, the cells are transferred to a 1.5 ml tube with 1 ml of TRIzol (Invitrogen, USA), 200 μl of Chloroform (Sigma, USA) is added, vortexed and left at room temperature for 5 minutes. After centrifugation at 15,000 rpm for 30 minutes, the supernatant is transferred to a new tube, mixed with an equal amount of 2-propanol, allowed to stand at room temperature for 5 minutes, and then centrifuged at 15,000 rpm for 20 minutes at 4 ° C. After centrifugation, discard 2-propanol, add 1 ml of 75% ethanol, centrifuge at 13,000 rpm for 10 minutes at 4 ° C, discard 75% ethanol, and remove remaining ethanol from the RNA pellet at room temperature. Pellet was dissolved in 80 μl of DEPC-treated purified water and quantitated using Qubit ™ fluorometer (Invitrogen, USA) and Qubit® RNA BR Assay Kit (Invitrogen, USA). Then, cDNA (complementary DNA) Respectively. For cDNA synthesis, cDNA synthesis kit (High Capacity RNA-to-cDNA Kit, Applied Biosystems, USA) was used. Real-time PCR was performed using the Real-time PCR Kit (Power SYBR Green PCR Master Mix, Applied Biosystems, CA, USA) and was performed according to the kit method. Using the Applied Biosystems 7500 FAST Real-Time PCR System, And amplified products were quantitatively analyzed. The MMP-1 and 36B4 primers used in PCR were synthesized in Bioneer (Korea) and the sequence was described below.

[primer sequence]

The reaction conditions are 15 sec at 95 ° C for 1 min and 40 cycles at 60 ° C for both MMP-1 and 36B4. EGCG used as a control is used as a substance that inhibits the expression of MMP-1. The results are shown in Table 2.

Test group Experimental concentration (μg / ml) MMP-1 expression inhibition amount (%) Example 1 0.1 24.76 10 73.58 Example 2 0.1 20.57 10 56.33 Example 3 0.1 13.09 10 30.25 Example 4 0.1 8.24 10 19.07 Control group EGCG 2.5 63.40 Untreated group - 0.00

As a result, as shown in Table 2, the inhibitory amount of MMP-1 mRNA expression was increased in all the concentrations of all the examples in comparison with the untreated group, and in Example 1, the expression inhibitory amount was increased more than that of the control group, EGCG, 1 inhibited collagen cleavage and inhibited wrinkle formation.

Experimental Example  3: Type  Ⅰ Collagen mRNA  Investigation of the expression level

The effect of type I collagen mRNA synthesis on the extract of Momordicae was investigated. To measure the expression of Type I collagen gene, Applied Biosystems 7500 FAST Real-Time PCR System (Applied Biosystems, CA, USA) was performed. Human normal fibroblasts (ATCC CRL-2076) were grown in IMDM medium (Gibco, USA) containing 10% bovine serum (Gibco, USA) at a density of 1 × 10 ⁶ in a 60 mm dish at 37 ° C. in a 5% CO ₂ incubator And cultured for one day. Subsequently, the medium is washed twice with HBSS (Hank's Balanced Salt Solution), then irradiated with UVA 6 J / cm ² , replaced with IMDM containing no bovine serum, added with the sample, and cultured at 37 ° C. for 24 hours. After incubation, the cells are collected in 1 ml of TRIzol (Invitrogen, USA), transferred to a 1.5 ml tube, added with 200 μl of Chloroform (Sigma, USA), vortexed and left at room temperature for 5 minutes. After centrifugation at 15,000 rpm for 30 minutes, transfer the supernatant to a new tube, mix with the same amount of 2-propanol, leave at room temperature for 5 minutes, and centrifuge at 4 ° C and 15,000 rpm for 20 minutes. After centrifugation, discard 2-propanol, add 1 ml of 75% ethanol, centrifuge at 13,000 rpm for 10 minutes at 4 ° C, discard 75% ethanol, and remove remaining ethanol from the RNA pellet at room temperature. Pellet was dissolved in 80 μl of DEPC-treated purified water and quantitated using Qubit ™ fluorometer (Invitrogen, USA) and Qubit® RNA BR Assay Kit (Invitrogen, USA). Then, cDNA (complementary DNA) Respectively. For cDNA synthesis, cDNA synthesis kit (High Capacity RNA-to-cDNA Kit, Applied Biosystems, USA) was used. Real-time PCR was performed using the Real-time PCR Kit (Power SYBR Green PCR Master Mix, Applied Biosystems, CA, USA) And amplified products were quantitatively analyzed. Type I collagen and 36B4 primer used in PCR were synthesized in Bioneer (Korea) and the sequence was described below.

[primer sequence]

The reaction conditions were 15 seconds at 95 ℃ for 1 minute, 40 cycles at 60 ℃ for both Type Ⅰ collagen and 36B4. EGCG, used as a control, is a substance that stimulates the activity of fibroblasts and promotes collagen synthesis. The measurement results are shown in Table 3.

Test group Experimental concentration (μg / ml) Type I expression of collagen (%) Example 1 0.1 160.86 10 233.57 Example 2 0.1 130.20 10 173.57 Example 3 0.1 104.20 10 113.57 Example 4 0.1 107.73 10 134.59 Control group EGCG 2.5 143.37 Untreated group - 100.00

As a result, as shown in Table 3, the expression level of Type I collagen mRNA was increased in a concentration-dependent manner in all the Examples, and in Example 1 and Example 2, the expression level of Type I collagen mRNA was higher than that of control EGCG, Promoting collagen synthesis.

Formulation Example  1: Flexible lotion ( skin )

As described below, the softened lotion (skin) containing the extract of ramie root obtained in Example 1 was prepared according to a conventional method with the composition shown in Table 4 below. Among the following raw material constituents, the extract of ramie root of the present invention is not limited to Example 1 but may be any one selected from Examples 1 to 4.

Raw material content Example 1 3.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) Hardened castor oil 1.00 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Formulation Example  2: Manufacture of nutrition lotion (lotion)

As described below, a nutritional lotion (lotion) containing the crude muscle root extract obtained in Example 1 was prepared in accordance with the usual method with the composition shown in Table 5 below. Among the following raw material constituents, the extract of ramie root of the present invention is not limited to Example 1 but may be any one selected from Examples 1 to 4.

Raw material content Example 1 1.0 Sitosterol 1.70 Polyglyceryl 2-oleate 1.50 Setares 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  3: Manufacture of nutritional cream

As shown below, the nutritional cream containing the morning glory extract obtained in Example 1 was prepared according to a conventional method with the composition shown in Table 6 below. Among the following raw materials, the ramipramus extract of the present invention is not limited to the examples but may be any one selected from Examples 1 to 4. [

Raw material content Example 1 1.0 Sitosterol 4.0 Polyglyceryl 2-olate 3.0 3.0 Setares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  4: Preparation of essence

Essences containing the morning glory extract obtained in Example 1 were prepared according to a conventional method as shown in Table 7 below. Among the following raw material constituents, the extract of ramie root of the present invention is not limited to Example 1 but may be any one selected from Examples 1 to 4.

Raw material content Example 1 1.0 Sitosterol 1.70 Polyglyceryl 2-oleate 1.50 Setares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  5: Manufacture of foundation

As described below, a foundation containing the crude muscle root extract obtained in Example 1 was prepared according to a conventional method with the composition shown in Table 8 below. Among the following raw material constituents, the extract of ramie root of the present invention is not limited to Example 1 but may be any one selected from Examples 1 to 4.

Raw material content Example 1 1.0 Wax 2.0 Cyclomethicone 2.0 Liquid paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic monostearic acid glycerin 3.0 Caprylic / capric triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butylene glycol 3.0 Triethanolamine 1.0 Aluminum magnesium silicate 0.5 Pigment 12 antiseptic a very small amount Spices a very small amount Purified water Balance

Test Example  1: Measurement of skin elasticity improvement effect by reduction of wrinkle depth

Formulation Example 3 (Formulation Example 3 and Comparative Formulation Example 3) were divided into two groups of 20 females in order to test the effect of the moxi root extract on the skin wrinkles. It was applied twice in the evening. The nutritional cream of Comparative Formulation Example 3 (Comparative Formulation Example 3) was used as a control group at the right eye margin. After 15 days and 30 days of application, the skin wrinkle depth on the skin surface was measured using Cutometer SEM 474. The skin elasticity measurement by Cutometer SEM 474 measures the depth of the wrinkles. The smaller the value, the better the resilience. The elasticity value of the test subjects is shown in%, and the mean value of the test subjects is shown in Table 9.

sample Skin elasticity (reduced wrinkle depth) Effect (%) 0 days 15th 30 days Execution Formulation Example 3 0 7.5 28.3 Comparative Formulation Example 3 0 2.4 9.4

As a result of the measurement, as shown in Table 9, when compared with that of the cosmetic product (Formulation Example 3) made from the crude extract (28.3%) 30 days after application and 9.4% It was confirmed that the wrinkle suppressing effect was excellent.

As described above, according to the present invention, the hairy root extract of the present invention can be used as a cosmetic composition for skin wrinkle suppression and improvement containing waxy root extract having an effect of inhibiting wrinkle formation and improving effect.

Claims (6)

A cosmetic composition for suppressing and improving wrinkle formation, comprising an extract of Panax notiens as an active ingredient.
The cosmetic composition according to claim 1, wherein the extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof.
The cosmetic composition for skin whitening according to claim 1, wherein the extract is contained in an amount of 0.1 to 10% by weight based on the total composition.
The cosmetic composition according to claim 1, wherein the composition exhibits an effect of inhibiting and improving wrinkle formation by inhibiting the expression of collagenase (MMP-1).
The cosmetic composition according to claim 1, wherein the composition exhibits an effect of suppressing and improving wrinkles by promoting collagen synthesis.
The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, Wherein the cosmetic composition has one formulation selected from the group consisting of a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.