KR20150113513A - Composition for whitening cosmetics comprising natural complex extracts with the effect of whitening - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising a natural whitening complex and, specifically, to a cosmetic composition for skin whitening, with skin whitening effects, antioxidant effects, anti-inflammatory effects, and excellent effects of skin cell regeneration, without skin stimulation, which comprises 10-30 wt% of fatty oil, 10-30 wt% of Centella asiatica Urbain, 10-20 wt% of blueberries, 10-20 wt% of Acanthopanax senticosus Harms, 5-10 wt% of Phellinus linteus, and 5-10 wt% of Inonotus Obliquus. The present invention relates to a cosmetic composition comprising a natural whitening complex, capable of significantly providing skin whitening effects, and comprising 0.01-20 wt% in a basic cosmetic product, a color cosmetic product, a cosmetic product for hair, a body cosmetic product, etc.

Description

TECHNICAL FIELD [0001] The present invention relates to a whitening cosmetic composition containing natural whitening complexes,

The present invention relates to a whitening cosmetic composition containing a natural whitening composite.

In recent years, interest in skin beauty has been increasing, and skin pigmentation such as spots and freckles in the skin is caused by factors such as ultraviolet rays, which are caused by melanin production or melanocyte proliferation. Pigmentation in the skin can be inhibited by reducing tyrosinase activity, which can reduce melanocyte function or reduce melanin production and inhibit pigmentation. In addition, it is known that melanin production can be reduced by inhibiting autoxidation reaction, and it is known that inhibition of pigmentation can be suppressed by suppressing inflammatory reaction such as erythema caused by ultraviolet irradiation (Case, DB, Wallace, JM, Keim, MA, Drayer, JM, White, RP, Sealey, JE and Laragh, JH: Estimating renin participation in hypertension: Superiority of converting enzyme inhibitor over saralasin, Am . J. Med ., 61, p.

Currently, whitening agents that are widely used in cosmetics worldwide include hydroquinone and arbutin, which are tyrosinase inhibitors. Recently, P & G (Procter & Gamble) reported deoxy arbutin, an arbutin derivative, and tried to develop derivatives such as arbutin in Korea.

Korean Patent No. 10-0535875 is directed to a whitening cosmetic composition using a plant mixture extract and is characterized by inhibiting tyrosinase activity and melanin production by B16 in order to inhibit melanin biosynthesis. Korean Patent No. 10-1086669 discloses a cosmetic composition for skin whitening comprising a Crassulaceae extract, wherein the diphosphine, saponin, and alkaloid components of the Crassula japonica root inhibit melanin production by inhibiting tyrosinase activity and DOPA oxidation activity in the skin Lt; / RTI > Korean Patent No. 10-0471685 relates to a whitening cosmetic composition comprising lactate and topical extract as an active ingredient, and relates to inhibiting melanin production without skin irritation.

The above-mentioned invention is merely to provide effects on inhibition of tyrosinase and inhibition of melanin production, and it has almost no effect and has a disadvantage that it is difficult to fundamentally improve. Therefore, there is a need for a cosmetic composition which can remarkably exert a whitening effect by improving various mechanisms such as melanin production inhibition, protection of skin cells due to oxidation, cell regeneration effect capable of normal growth and turnover of cells .

The inventors of the present invention have conducted studies to develop a cosmetic composition having no skin irritation and excellent whitening effect, and as a result, it has been found that a cosmetic composition comprising a natural combination extract of Jiu-yu, Cilotheri, Blueberry, Acanthopanax senticosus, Antioxidative effect, antiinflammatory effect and cell regeneration effect are excellent. Thus, the present invention has been completed.

KR 10-0535875 2005.12.05. KR 10-1086669 Nov. 18, 2011.

Accordingly, the inventors of the present invention discovered a whitening composition having no whitening effect, whitening effect, antioxidative effect, antiinflammatory effect, and cell regeneration effect while studying a cosmetic composition for imparting a whitening effect, and completed the present invention.

Accordingly, it is a technical object of the present invention to provide a whitening cosmetic composition containing a natural whitening composite.

In order to achieve the above object, the present invention provides a whitening cosmetic composition containing 0.01 to 20% by weight of a natural compound extract and capable of remarkably imparting a whitening effect.

The whitening cosmetic composition containing the natural whitening composition may be a skin, a lotion, an essence, a serum, a cream, an eye cream, a mask pack, a foam cleanser, a makeup, a two-way cake, a babe cream, And a pharmaceutically acceptable carrier.

The whitening cosmetic composition containing the natural whitening composite according to the present invention is free of skin irritation, has excellent whitening effect, antioxidative effect, cell regeneration effect, and satisfies the user with no trouble and irritation.

1 is a DPPH inhibitory activity antioxidative activity of the natural whitening composite prepared in Examples 1 and 2;
Fig. 2 is a SOD-like activity graph showing the antioxidative activity of the skin whitening cosmetic composition prepared in Examples 1 and 2. Fig.
Fig. 3 is a graph showing anti-inflammatory results of the cosmetic composition for skin whitening produced in Example 1. Fig.
4 is a graph showing anti-inflammation results of the cosmetic composition for skin whitening produced in Example 1. Fig.
5 is a graph of cytotoxicity results of the skin whitening cosmetic compositions prepared in Examples 1 and 2;
FIG. 6 is a graph showing the results of tyrosinase inhibition activity as a whitening effect of the cosmetic composition for skin whitening prepared in Examples 1 and 2. FIG.

Hereinafter, the present invention will be described in detail.

The whitening cosmetic composition containing the natural whitening complex of the present invention includes an extract of the oil palm, the cinnabar, the blueberry, the sea bass, the mushroom and the chaga mushroom.

It is a perennial plant belonging to the rose family, which is widely distributed in China, Japan, and all parts of Korea. There is a record used in traditional Chinese medicine for the treatment of burns, inflammation and internal bleeding. The phytonutrients of zygus contains the ziguglycoside I, II and pomolic acid, which have excellent antioxidative and whitening activity. In the present invention, the coarse oil is preferably mixed in an amount of 10 to 30% by weight based on the total dry weight of the composition to be mixed. When the coarse oil is mixed in an amount of less than 10% by weight, There is a problem of physical properties such as discoloration and lowering of viscosity.

Centella asiatica is a perennial herbaceous herbaceous plant of the dicotyledonous herbaceous plant of the dicotyledonous plant family. It grows in a somewhat moist place. Its root extends from side to side and has scaly leaves. Cell regeneration effect and anti-inflammatory effect are excellent. In the present invention, it is preferable to mix 10 to 30% by weight based on the total dry weight of the composition to be mixed, and there is a problem that little effect is exhibited. When the composition is mixed in an amount of more than 30% by weight, There is a property problem.

The blueberry has an anthocyanin color, blue sugar, sour sweet sugar, viscous pectin, and a fragrant scent. Fruit and leaf extract have been used for the treatment of scurvy, diabetes, and urinary disease, and it is known that they have a strong antioxidant effect by anthocyanins in recent studies. In the present invention, the blueberries are preferably mixed in an amount of 10 to 20% by weight based on the total dry weight of the composition to be mixed. When less than 10% by weight is mixed, the effect is hardly exhibited. There is a problem of physical properties such as discoloration and viscosity decrease.

It is also known as Acanthopus spp., And it grows in deep valleys throughout the country. Antioxidant, anti-inflammatory, and atopic skin. In the present invention, the viscose is preferably mixed in an amount of 10 to 20% by weight based on the total dry weight of the composition to be mixed. When the content is less than 10% by weight, the effect is hardly exhibited. There is a problem of physical properties such as discoloration and lowering of viscosity.

The above-mentioned mushroom is also referred to as woody mud mushroom, and it is recorded in the name of mushroom in the name of " It is known that the anticancer effect is excellent, and the antioxidative and whitening effect is excellent. In the present invention, the mushroom is preferably mixed in an amount of 5 to 10% by weight based on the total dry weight of the composition to be mixed. When the mushroom is mixed in an amount of less than 5% by weight, There is a problem of physical properties such as discoloration and viscosity decrease.

The mushroom is a mushroom parasitized on birch of Siberia, North America, northern Europe and more than 45 degrees north latitude, and is excellent for the treatment of adult diseases such as cancer. The pharmacological activity of cosmetics is characterized by excellent moisturizing and soothing effects and excellent whitening effect. In the present invention, chaga mushrooms are preferably mixed in an amount of 5 to 10% by weight based on the total dry weight of the composition to be mixed. When mixed in less than 5% by weight, the effect is hardly exhibited. There is a problem of physical properties such as discoloration and viscosity decrease.

The natural whitening complex according to the present invention,

10 to 30% by weight of ground oil, 10 to 30% by weight of blueberry, 10 to 20% by weight of blueberry, 10 to 20% by weight of gooseberry, 5 to 10% by weight of mushroom and 5 to 10% do.

The method for preparing the natural whitening complex composition according to the present invention is as follows.

The method for producing a natural whitening complex composition of the present invention comprises:

10 to 30% by weight of ground oil, 10 to 30% by weight of blueberry, 10 to 20% by weight of blueberry, 10 to 20% by weight of gooseberry, 5 to 10% by weight of mushroom and 5 to 10% Step (step 1);

Extracting the mixture with water or an aqueous ethanol solution to prepare an extract (step 2); And

Filtering and concentrating the extract to prepare a natural whitening complex composition (step 3);

.

In step 1, the natural raw materials are preferably washed with water, dried, and then finely mixed.

In step 2, 8 to 10 times volume of water is added to the mixture, followed by hot extraction at 80 to 85 for 2 to 4 hours, or 8 to 10 times volume of an aqueous ethanol solution is added to the mixture, Solvent extraction is preferred. The ethanol aqueous solution preferably has a volume ratio of ethanol and water of 7: 3.

The hot water extraction and the solvent extraction are preferably repeated three times.

In step 3, the filtrate is preferably passed through a filter paper to remove the residue, and then concentrated using a rotary vacuum concentrator at 40-45 for 6-8 hours.

On the other hand, the whitening cosmetic composition containing the natural whitening composite according to the present invention can be prepared by containing 0.01 to 20% by weight, preferably 0.1 to 10% by weight, based on the total weight of the natural whitening complex composition . When the natural whitening composite composition is contained in an amount less than 0.01% by weight, there is a problem that the whitening effect is insignificant. When the natural whitening complex composition is used in an amount exceeding 20% by weight, the physical properties of the final product are deteriorated and discoloration occurs.

The whitening cosmetic composition containing the natural whitening composition may be a skin, a lotion, an essence, a serum, a cream, an eye cream, a mask pack, a foam cleanser, a makeup, a two-way cake, a babe cream, And a pharmaceutically acceptable carrier.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

Example 1

250g of jelly, 250g of blueberry, 150g of blueberry, 150g of Ganoderma Lucidum, 100g of Shiitake mushroom and 100g of Shiitake mushroom were washed with water, dried to less than 10% moisture content, and finely divided into 1 to 3 pieces to prepare a mixture.

10 times volume of water was added to the mixture, and the mixture was subjected to hot extraction for 3 hours at 85 ° C, and extracted three times to obtain an extract.

The extract was passed through a filter paper (Adventec No. 2) to remove the residue, and then concentrated using a rotary vacuum concentrator (HS-10P, Hanshin) at 45 for 8 hours to prepare a natural whitening composite composition.

Example 2

(Ethanol: water = 7: 3 by volume) was added to the above mixture instead of hot water extraction in Example 1, and the mixture was extracted for 25 to 24 hours. , A cosmetic composition for skin whitening was prepared.

[Experimental Example 1]

The electron donating ability was measured to examine the antioxidative effect of the skin whitening cosmetic composition prepared in Examples 1 and 2. BHA (butylated hydroxyanisole), a synthetic antioxidant, was used as a control.

The electron donating ability (EDA) was measured by the modified Blois method (Blois, M. S.: Antioxidant determination by the use of a stable free radical. Nature, 181, 1190-1200, 1958) as follows. 0.2 mM DPPH (1,1-diphenyl-2-picryl-hydrazyl) 50 was added to a sample solution 100 having a constant concentration, and after stirring for 30 minutes at room temperature, the absorbance at 517 was measured and shown in FIG.

1 shows that the skin whitening cosmetic compositions of Examples 1 and 2 exhibited antioxidative activities similar to those of synthetic antioxidant (BHA) as a control group, and that the ethanol aqueous solution extract of Example 2 was similar to Example 1 Which is an effective antioxidant activity, than the water extract of the present invention.

[Experimental Example 2]

To examine the antioxidative effect of the skin whitening cosmetic compositions prepared in Examples 1 and 2, SOD-like activity (Superoxide Dismutade-like Activity) was measured. The control group was BHA.

SOD-like activity was measured by the method of Marklund et al. (Marklund, S. and Marklund, G.: Involvement of superoxide anion radicals in pyroalloy and a convenient assay for superoxide desmutase, Eur. J. Biochem., 47, 468, 1974) . The reaction was stopped by adding 25% Tris-HCl buffer solution (50 mM tris + 10 mM EDTA, pH 8.5) 260 and 7.2 mM pyrogallol 20 to the sample solution 20 at 25 for 10 minutes and then stopping the reaction by adding 5N HCl 10, The amount of pyrogallol was measured at 420 and is shown in Fig.

FIG. 2 shows that the extract of aqueous ethanol (ethanol) of Example 2 at 500 ppm or higher exhibited similar activity to BHA as the control group, indicating strong SOD-like activity.

[Experimental Example 3]

In order to examine the anti-inflammatory effects of the skin whitening cosmetic compositions prepared in Examples 1 and 2, Nitric Oxide inhibitory activity was measured. Lipopolysaccharide (LPS) was used to stimulate inflammation.

The amount of nitric oxide produced from Raw 264.7 cells was determined by the method of Byun, SH, Yang, CH and Kim, SC: Inhibitory effect of scrophulariae radix extract on TNF-a, IL-1, IL-6 and Nitric Oxide production in lipopolysaccharide-activated mouse macrophage Raw 264.7 cells. Kor J. Ori. Herbology., 20, 7-16, 2005). NO ² present in the cell culture ^- was measured using the Griess reagent. Cell culture supernatant 100 and griess reagent 100 were mixed and reacted for 10 minutes and absorbance was measured at 540. The amount of NO ^{2 -} was quantified using the standard curve of NaNO ₂ .

The anti-inflammatory effect of the water extract of Example 1 is shown in FIG. 3, and the anti-inflammatory effect of the aqueous ethanol extract of Example 2 is shown in FIG.

3 and 4, it can be seen that as the content of both the hot water extract of Example 1 and the ethanol aqueous solution of Example 2 increases, inflammation sinks more in the LPS-treated group than that of stimulation.

[Experimental Example 4]

Cytotoxicity tests were carried out on the skin whitening cosmetic compositions prepared in Examples 1 and 2. The effect of the sample on Raw 264.7 cells was evaluated by the method of Carmichael et al. (Carmichael, J., De Graff, WG, Gazdar, AF, Minna, JD and Mitchell, JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay. Cancer Res., 47, 936-942, 1987). The cells were seeded at 50,000 cells / well on a 96-well plate, and when the confluency reached 80%, the original medium was removed and cultured with serum-free EMEM. After 12 hours of incubation, samples were added at different concentrations and incubated in 37, 5% CO ₂ incubator. The control group was cultured under the same conditions by adding the same amount of distillation as the sample. After 4 hours, the supernatant was removed, and the reaction was carried out at room temperature for 30 minutes in DMSO: EtOH (1: 1) per well. The absorbance of the MTT solution was measured at 550 using an ELISA reader Were measured.

 The results of confirming the survival rate of macrophage cells by ethanol extract by MTT assay are shown in Fig.

As shown in FIG. 5, it was determined whether or not the cell population was caused by a decrease in the cell population due to cytotoxicity.

[Experimental Example 5]

In order to examine the whitening effect of the skin whitening cosmetic compositions prepared in Examples 1 and 2, the tyrosinase inhibitory activity was measured.

Tyrosinase inhibitory activity was measured according to the method of Yagi et al. (Yagi A, Kanbara T, Morinobu N. The effect of tyrosinase inhibition for aloe, Planta Medica ., 1986, 3981: 517-519). To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA was dissolved in 0.5 mL of sodium phosphate buffer (pH 6.8) and 0.1 mL of the sample solution, The DOPA chrome produced in the reaction solution was measured at 475 nm. Tyrosinase inhibitory activity was expressed as the absorbance reduction ratio of the sample solution and the no - sample group.

The results of the experiment are shown in FIG. 6. As Tyrosinase inhibitory activity is increased as the content of the natural complex extract is increased, it is confirmed that it is effective for skin whitening.

Example 3

Ethanol extract was prepared as shown in Table 1.

[Comparative Example 1]

A general essence composition containing no ethanol extract was prepared as shown in Table 1.

Component (% by weight) Example 3 Comparative Example 1 EDTA-2Na 0.02 0.02 Butylene Glycol 5 5 Glycerin 3 3 Sodium Hyaluronate One One DPG-K2 0.05 0.05 Allantoin 0.1 0.1 Betaine 5 5 Carbomer 0.1 0.1 TEA 0.1 0.1 Xanthan Gum 0.05 0.05 Panthenol 0.1 0.1 Hydrolyzed Collagen One One Methyl Paraben 0.15 0.15 Propyl Paraben 0.02 0.02 Spices One One Whitening compound extract composition 0.1 - Purified water To 100 To 100

[Experimental Example 6]

The total aerobic bacteria experiment of the cosmetic composition containing the natural complex extract for whitening improvement prepared in Example 3 was carried out.

Total aerobic viable count is a test method to measure the total aerobic viable count (bacteria, fungi) in cosmetics. The total aerobic viable count is the sum of the detected number of bacteria and the total number of fungi. The total aerobic count is used for modified letheen broth or soybean casein digestion culture medium. The fungus count test is performed using potato dex Trough agar medium or antibiotic added sucrose agar medium was used. The results of the experiment are shown in Fig. 7. In the cosmetic composition containing the natural complex extract for whitening improvement prepared in Example 2, no bacteria or fungi were found.

[Experimental Example 7]

The skin safety test of the cosmetic composition containing the natural complex extract for whitening improvement prepared in Example 3 was carried out.

Skin safety test of patch test of the cosmetic composition containing natural extract composite whitening improvement is Waggoner etc. (Waggoner WC, etc. Clinical safety and efficacy testing of cosmetic. Cosmetic science and Technology series. New York , Marcel Dekker . 1990: 105), the cosmetic composition containing the natural complex extract for whitening improvement prepared in Example 2 was applied to the skin on the inside of the plate using a Finn chamber on a Scanpor tape for 24 hours, The results were compared with those of the control group.

The results of the patch test are shown in Table 3, and the adult male and female were confirmed to be negative and the skin stability was confirmed.

Reaction Expression Negative - Imperfection erythema + - Erythema + Small blister, Papule, Edema ++ Big blister, Necrosis +++

Group No. of patch tested 24hour Femal 50

50 * - Male * -: Negative

Accordingly, the skin whitening complex extract cosmetic composition according to the present invention is an epoch-making composition which is excellent in antioxidant and anti-inflammation, has no cytotoxicity, and has excellent whitening effect, thereby making the skin bright and healthy.

Claims (6)

Wherein the natural whitening composition comprises at least one member selected from the group consisting of soybean oil, coconut oil, cinnabar, blueberry, gooseberry, mushroom and chaga mushroom extract. The method according to claim 1,
The extract was prepared by mixing 10 to 30% by weight of a fat base oil, 10 to 30% by weight of a herbage spoon, 10 to 20% by weight of a blueberry, 10 to 20% by weight of a sea bass, 5 to 10% by weight of a mushroom, and 5 to 10% ≪ / RTI > wherein the composition is an extract of the mixture. 10 to 30% by weight of ground oil, 10 to 30% by weight of blueberry, 10 to 20% by weight of blueberry, 10 to 20% by weight of gooseberry, 5 to 10% by weight of mushroom and 5 to 10% Step (step 1);
Extracting the mixture with water or an aqueous ethanol solution to prepare an extract (step 2); And
Filtering and concentrating the extract to prepare a cosmetic composition for skin whitening (step 3);
≪ / RTI > or a pharmaceutically acceptable salt thereof. 4. The method of claim 3, wherein in step 2,
8 to 10 times volume of water is added to the mixture, followed by hot extraction at 80 to 85 ° C for 2 to 4 hours, or 8 to 10 times volume of an aqueous ethanol solution is added to the mixture, followed by solvent extraction at 20 to 25 for 20 to 24 hours ,
Wherein the ethanol aqueous solution has a volume ratio of ethanol to water of 7: 3. A cosmetic composition for whitening, which comprises 0.01 to 20% by weight of the whitening cosmetic composition according to claim 2, based on the total weight of the composition. 6. The method of claim 5,
The method for producing a cosmetic composition for skin whitening includes a skin, a lotion, an essence, a serum, a cream, an eye cream, a mask pack, a foam cleanser, a makeup, a two-way cake, a bib cream, a sunscreen, a body care product, Wherein the cosmetic composition is a cosmetic composition.

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