KR20150106804A - Personal cleansing composition comprising Magnolia officinalis extract - Google Patents

Abstract

The present invention relates to a composition for cleaning a human body including an Officinalis extract and, more specifically, to a body cleaning composition including Officinalis extract, fatty acid and a flavor having octanol-water partition coeffitient (ClogP) of three or more. The body cleaning composition has excellent effect of controlling propagation and removing body smell-causing microorganisms, and has excellent antibacterial power for acne germ to effectively treat acne, and has excellent scent residue. Also, the composition for body cleaning is formed with natural components to be safe to a human body, thereby being used from maintaining and improving skin health regardless of age.

Description

[0001] The present invention relates to a personal cleansing composition comprising Magnolia officinalis extract,

The present invention relates to a human body cleansing composition containing an extract of Zygopta japonica. More particularly, the present invention relates to a human body cleansing composition having excellent antimicrobial effect against major microorganisms causing acne and having excellent reverberation.

Among skin diseases, the onset and deterioration of body odor and acne, which are of interest to the present invention, are closely related to microorganisms, especially bacteria. First, body odor is caused by microorganisms decomposing the eccrine sweat, apocrine sweat, sebum, or contaminants secreted from the human body. The first secreted eczrine sweat or apocrine sweat is odorless, but it causes a body odor such as odor, jaws, and dodeca that cause discomfort to humans by the action of many microorganisms such as skin epidermis, hair, pores, especially microorganisms related to body odor.

Skin, to the number of flora present, typical to the Staphylococcus aureus is Staphylococcus (Staphylococcus), Streptococcus micro Rhodococcus (Micrococcus), Corynebacterium (Corynebacterium), P ET Los Forum (Pityrosporum), or E. coli (E .coli ), and in particular, it is known that Corynebacterium is found in a lot of people who give a strong smell to the nose stimulating the nose. Next, acne is a chronic inflammatory disease of the epidermis that occurs mainly on the face, chest, and neck of young men and women, such as adolescents, and may cause comedones, papules, pustules, cysts, or nodules In severe cases, it is a lesion that can leave a scar.

The mechanism of acne formation has not yet been clarified yet. However, it is generally believed that 1) acne bacterium proliferation, 2) sebaceous secretion increase, 3) abnormally increased hair follicle wall hypersensitivity, and 4) , And the acne treatment method has the principle of eliminating these causes.

There is a change in the lipid content of sebum in acne patients. The content of linoleic acid and cholesterol in acyl ceramide decreases and the content of squalene and fatty acids increases. This change in fat composition results in an imbalance between cholesterol and cholesterol sulfate in the hair follicle, thereby increasing the association between the keratinocytes in the hair follicle, resulting in follicular retention hyperkeratosis. As a result, the sebum stagnated in the hair follicles blocks the air circulation by blocking the hair follicle, and thus the inside of the hair follicle becomes an environment where the anaerobic bacteria, Propionibacterium acnes , which resides in the hair follicle, can grow well. The acne bacterium secretes lipolytic enzymes and chemotactic factors to produce fatty acids, which cause white blood cells to gather around the hair follicle, which stimulates and destroys the hair follicles, causing inflammation as the contents of the hair follicles drain into the dermis.

Many antimicrobial substances that inhibit the body odor and the propagation of acne bacterium are known, such as triclosan, triclocarban, benzalkonium chloride, benzethonium chloride, chlorhexidine hydrochloride, Chlorhexidine gluconate, fanesol, copper gluconate, or zinc gluconate; Plant oil or plant extracts such as tea tree oil, clove, fennel, peppermint, or gossam; Or chitin or a derivative thereof.

However, these substances do not exhibit sufficient antibacterial effect against acne bacterium only by a small amount, and there is a fear that side effects such as skin redness or dryness of skin may occur when overdosing, and antimicrobial substances popularized such as trichloroacetate and trichloracane are used for a long time It has been reported that the resistance to pathogens in the skin is increased and endocrine hormone disturbance and carcinogenicity are reported, and it is difficult to exhibit a sufficient antimicrobial effect due to the lack of skin adsorption ability for the washed product.

Accordingly, a problem to be solved by the present invention is to provide a composition for human body cleansing which shows strong antimicrobial effect on body odor bacteria and acne bacterium including skin onychomycetes, has excellent skin adsorption degree after cleansing, .

In order to solve the above-mentioned problems, the present invention provides a human body cleansing composition comprising a crude extract, a fatty acid, and a perfume having a ClogP (octanol-water partition coefficient) value of 3 or more according to the following formula do.

[Equation 1]

The inventors of the present invention have found that when the composition for human body cleansing which is excellent in skin hygiene promotion, unpleasant body odor removal, and acne improvement effect is developed, it is found that the extract of L. elata has a strong antibacterial effect on microorganisms causing body odor and acne Respectively. In addition, when the composition for human body cleansing comprises a combination of a fatty acid and an oil having a ClogP value of 3 or more together with the fatigue extract, the fatigue of the fatigue extract is increased and the reverberation is excellent even after cleansing.

In the present invention, the nutmeg extract may be obtained by extracting from various organs or parts of the ginger tree, that is, leaves, flowers, fruits, stems, roots, branches, or bark. The extract can be obtained by a solvent extraction method using water or an organic solvent, a supercritical extraction method, a steam distillation method, an ultrasonic extraction method or the like as an extraction method commonly used in the art to which the present invention belongs. Preferably, the extract can be extracted using supercritical extraction using carbon dioxide.

As the organic solvent that can be used in the solvent extraction method, an organic solvent commonly used in the art can be used, and lower alcohols such as methanol, ethanol, isopropyl alcohol, and butanol; Polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol; And hydrocarbon solvents such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform, and the like.

The after-treatment extract may be a liquid extract obtained by a solvent extraction method or a solution thereof diluted in a solvent, a supercritical extract using carbon dioxide, or a powder obtained by using the supercritical extract. The pulverization may be carried out by conventional methods known in the art such as vacuum drying, freeze drying, or spray drying. Preferably the supernatant extract may be a supercritical extract using carbon dioxide.

The extract of the present invention includes not only the above-mentioned extraction solvent, but also an extract which has been subjected to a conventional purification process. Specifically, the extract of the present invention can be used for various purification processes such as a purification process after extraction with an extraction solvent, for example, a membrane separation using a filtration membrane having a cut-off value at a constant molecular weight, separation for size, charge, hydrophobicity, A purification process such as separation by chromatography may be additionally carried out.

In the present invention, the nutmeg extract may be contained in an amount of 0.01 to 2% by weight, preferably 0.02 to 1.5% by weight, more preferably 0.03 to 0.8% by weight, based on the total weight of the composition. If the content of the aftercoat extract is less than 0.01% by weight, no antibacterial activity is exhibited. If the content is more than 2% by weight, economic efficiency and formulation stability are not preferable.

In the composition of the present invention, the fatty acid is selected from the group consisting of C8-20 carboxylic acids including lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid and the like derived from animal fats or plant fats; Fatty acids derived from vegetable oils including palm oil, palm kernel oil, soybean oil, rape oil, corn oil, rapeseed oil, sunflower oil, safflower oil, cottonseed oil, sesame oil, rice bran oil, camellia oil, castor oil, olive oil and palm oil; Fatty acids derived from animal fats (e. G., Tallow acid) including tallow, lard, sunfish, fish oil, whale oil, and tuna oil; Fatty alcohols, mineral oils, paraffin, and the like may be used, but the present invention is not limited thereto. Preferably, tallow acid derived from palm oil fatty acid, palm kernel oil fatty acid, or tallow oil derived from palm oil or palm kernel oil is used as the fatty acid.

In the present invention, the fatty acid is preferably added in an amount of from 1 to 30% by weight, preferably from 2 to 15% by weight, more preferably from 2 to 15% by weight based on the total weight of the composition, in order to increase the adsorption on the skin and impart an excellent emollient effect, Preferably 3 to 7% by weight. When the content of the fatty acid is less than 1% by weight, the skin adsorption effect is markedly deteriorated. When the content is more than 30% by weight, the feeling and stability are not good.

The perfume having a ClogP value of 3 or more in the composition of the present invention is excellent in reverberation due to high skin persistence even after cleaning. In the composition of the present invention, the fragrance having a ClogP value of 3 or more may be contained in an amount of 0.1 to 5% by weight, preferably 0.5 to 3% by weight based on the total weight of the composition. If the content of the perfume is less than 0.1% by weight, the effect of reverberation is markedly deteriorated. If the content exceeds 5% by weight, the formulation stability is deteriorated.

The present invention also provides a human body cleansing composition comprising 0.01 to 2% by weight of an extract of the extract of the present invention and 1 to 30% by weight of a fatty acid based on the total weight of the composition, and a perfume having a ClogP value of 3 or more. For the purposes of the present invention, the composition comprising the combination of the specific weight ratio components has the best antimicrobial activity and reverberation against odoriferous microorganisms or acne bacteria.

The composition for human body cleaning of the present invention is further classified into a group consisting of a higher fatty acid alkali salt, a surfactant, a wetting agent, a metal ion sequestering agent, and purified water for the purpose of improving human body washing effect, formulation stability, desired effect, Based on the total weight of the composition.

The higher fatty acid alkaline salt may serve to provide a good cleaning effect. As the higher fatty acid, at least one selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, and oleic acid is used But is not limited thereto. The higher fatty acid alkali salt may be contained in an amount of 5 to 80% by weight based on the total weight of the composition.

The surfactant may be used as a foam promoter, and at least one selected from the group consisting of an anionic surfactant, a nonionic surfactant, and an amphoteric surfactant may be used.

The anionic surfactant is not particularly limited as long as it can be used in a conventional cosmetic composition, and includes fatty acid soap (sodium laurate, sodium palmitate, etc.); Higher alkyl sulfate ester salts (sodium lauryl sulfate, potassium lauryl sulfate and the like); Alkyl ether sulfuric acid ester salts (POE-laurylsulfuric triethanolamine, POE-laurylsulfate, etc.); N-acyl sarcosinate (such as sodium lauroyl sarcosine); Higher fatty acid amide sulfonates (N-myristoyl-N-methyltaurin sodium, coconut fatty acid methyltauride sodium, laurylmethyltauride sodium and the like); Phosphoric acid ester salts (sodium POE-oleyl ether phosphate, POE-stearyl ether phosphoric acid, etc.); Sulfosuccinic acid salts (sodium di-2-ethylhexylsulfosuccinate, sodium monooroyl monoethanolamide polyoxyethylene sulfosuccinate, sodium lauroyl polypropylene glycol sulfosuccinic acid and the like); Alkylbenzenesulfonates (sodium linear decylbenzenesulfonate, triethanolamine linear decylbenzenesulfonate, linear decylbenzenesulfonic acid, etc.); Higher fatty acid ester sulfuric acid ester salts (such as hardened coconut oil fatty acid glycerin sodium sulfate); N-acyl glutamate (monosodium N-lauroyl glutamate, disodium N-stearoyl glutamate, monosodium N-myristoyl-L-glutamic acid, etc.); Sulfuric acid oil (lot oil, etc.); POE-alkyl ether carboxylic acid; POE-alkyl allyl ether carboxylate; ? -olefin sulfonate; Higher fatty acid ester sulfonates; Secondary alcohol sulfuric acid ester salts; Higher fatty acid alkylolamide sulfates; Sodium lauroyl monoethanolamide succinate; N-palmitoyl aspartic acid ditriethanolamine; And sodium caseinate can be used, but the present invention is not limited thereto.

As the nonionic surfactant, a lipophilic nonionic surfactant or a hydrophilic nonionic surfactant can be used and is not particularly limited as long as it can be used in a common cosmetic composition. Examples of the lipophilic nonionic surfactant include sorbitan fatty acid Esters (such as sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, penta-2 - ethylhexyl acid diglycerol sorbitan, tetra-2-ethylhexyl acid diglycerol sorbitan and the like); Glycerin polyglycerin fatty acids (monosaccharic fatty acid glycerin, monoelucic acid glycerin, sesquioleic acid glycerin, monostearic acid glycerin,?,? '- oleic acid pyroglutamic acid glycerin, monostearic acid glycerin malic acid, etc.); Propylene glycol fatty acid esters (such as propylene glycol monostearate); Hardened castor oil derivatives; Sorbitan fatty acid esters (POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan tetraoleate and the like); hydrophilic nonionic surfactants such as polyoxyethylene sorbitan fatty acid esters POE-sorbit fatty acid esters (POE-sorbitol monolaurate, POE-sorbitol monooleate, POE-sorbitol pentaoleate, POE-sorbitol monostearate, and the like); POE-glycerin fatty acid esters (POE-glycerin monostearate, POE-glycerin monoisostearate, POE-glycerin triisostearate, etc.); POE-fatty acid esters (POE distearate, POE-monodioleate, ethylene glycol distearate, etc.); POE-alkyl ethers (POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether, POE-cholestanol ether, etc.); Pluronic molds (Pluronic and the like); POE / POP-alkyl ethers (POE / POP-cetyl ether, POE / POP-2-decyltetradecyl ether, POE / POP-monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether and the like); Tetra POE tetra POP-ethylenediamine condensation products (such as Tetronic); POE-castor oil-hardened castor oil derivative (POE-castor oil, POE-hardened castor oil, POE-hardened castor oil free monoisostearate, POE-hardened castor oil free triisostearate, POE-hardened castor oil free monopyrroglutamate monoisoprene Stearic acid diester, POE-hardened castor oil free maleic acid); POE beeswax / lanolin derivatives (POE-sorbitol beeswax); Alkanolamides (palm oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE-propylene glycol fatty acid esters; POE-alkylamines; POE-fatty acid amide; Sucrose fatty acid esters; Alkylethoxydimethylamine oxides; And trioleyl phosphoric acid, and the like, and at least one selected from the group consisting of these may be used, but the present invention is not limited thereto.

In addition, the present invention may contain an amphoteric surfactant to assist in the detergency and to maintain an abundant foam. The amphoteric surfactant is not particularly limited as long as it can be used in a conventional cosmetic composition and includes an imidazoline type amphoteric surfactant (2-undecyl-N, N, N- (hydroxyethylcarboxymethyl) -2- Sodium 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy disodium salt, and the like); And a betaine surfactant (2-heptadecyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, alkylbetaine, amide betaine, sulfobetaine etc.) May be used. However, the present invention is not limited thereto.

In the present invention, the surfactant may be contained in an amount of 0.1 to 30% by weight based on the total weight of the composition.

The wetting agent may be used for imparting a moisturizing effect to the skin after cleansing, and any one or more selected from the group consisting of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycols, and propylene glycol may be used . In the present invention, the wetting agent may be used in an amount of 0.1 to 20% by weight based on the total weight of the composition.

In the composition of the present invention, the sequestering agent is a substance which inhibits the shrinkage of the composition by chelating metal ions to prevent the oxidation of the fatty acid, and phosphate-based compounds and edetate may be used. More specifically, the sequestering agent is selected from the group consisting of sodium phosphate, monosodium phosphate, disodium phosphate, trisodium phosphate (tetrasodium etidronate) Sodium triphosphate, sodium hexametaphosphate, tetrasodium pyrophosphate, sodium acid pyrophosphate, disodium EDTA, and tetrasodium diiodide, Tetrasodium EDTA, etc. The metal ion sequestrant may be used in an amount of 0.01 to 0.5% by weight based on the total weight of the composition.

In the composition for human body cleaning according to the present invention, the extract of L. extract which acts as an active ingredient may contain Magnolol and Honokiol as main components in a weight ratio of about 1: 1. The magnolol and honokiol may be contained in an amount of 90% by weight or more based on the total weight of the crude extract.

The human body cleansing composition according to the present invention may comprise a mixture of L. extract and a fatty acid in a weight ratio of 1:10 to 50 (L. extract: fatty acid) and a fragrance having a ClogP value of 3 or more. Preferably, the weight ratio is 1:20 to 40 (baklava extract: fatty acid). When the weight ratio of fatty acid is less than 1:10 by weight of the mixture, it is difficult to expect an increase in antimicrobial effect due to a low residual skin concentration of the extract. In addition, when the weight ratio of the fatty acid is more than 1:50 by weight of the mixture, the fatty acid is easy to be removed, bubbles are reduced, and the fragrance is strong. In addition, the human body cleansing composition according to the present invention may comprise a mixture of a rosemary extract, a fatty acid, and a fragrance having a ClogP value of 3 or more at a weight ratio of 1: 10: 5 to 1:50:25. The composition comprising the above mixture is remarkably excellent in not only the antibacterial activity against harmful bacteria but also the reverberation for the purpose of the present invention.

The mixture may contain 1 to 35% by weight, preferably 3 to 25% by weight based on the total weight of the composition. When the amount of the mixture is less than 1% by weight, the antibacterial effect is insignificant and a desired effect can not be expected. When the amount is more than 35% by weight, skin irritation such as itching may be caused and the preparation thereof is not easy.

The composition for human body cleaning according to the present invention is excellent in antimicrobial effect against acute causative bacteria and acne bacteria. More specifically, the composition has excellent proliferation inhibiting and eliminating effects against Corynebacterium xerosis , which is an active cause of acne, and Propionibacterium acnes, which is an acne bacterium, .

In addition, the composition for human body cleaning according to the present invention may be prepared as a liquid, an ointment, or a solid for the purpose of proper formulation for convenience of use. More specifically, the composition of the present invention may be contained in a product for cleaning face, skin or hair such as cosmetic soap, body wash, cleansing foam, shampoo, rinse, or conditioner, but is not limited thereto.

The present invention also provides a method for preparing a human body cleansing composition having an improved antimicrobial effect by increasing the skin adsorption rate of an extract of Lycoris sp. In the above method, the fatty acid is bound to the extract of Lycoris spp. To increase the amount of skin adsorbed after cleansing, thereby remarkably increasing the antimicrobial effect of Lycopersicum esculentum extract against the causative bacteria and acne bacteria. The method may comprise a mixture of the backbone extract and the fatty acid in a weight ratio of 1:10 to 50 (backbone extract: fatty acid) and a perfume having a ClogP value of 3 or more.

INDUSTRIAL APPLICABILITY The human body cleansing composition of the present invention is excellent in suppressing and eliminating the proliferation and odor of body odor-inducing microorganisms such as odor, jaws and dodec, and is excellent in antibacterial activity against acne bacteria and is effective in treating acne and has excellent reverberation . In addition, since the composition for human body cleaning of the present invention is composed of natural ingredients and is safe for human body, it can be used for maintenance and improvement of skin health regardless of age.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

The inventors of the present invention have conducted studies on fatty acids which are obtained by dissolving ginger extracts having a ClogP value of not less than 3 in the fragrance extracts having the best overall antimicrobial effect for easy application of human cleansing base material and emollient effects in an oil phase, To investigate the effect of the extracts, we tested the antimicrobial effect of the human body washing substrate by measuring the skin residual concentration of the foaming extract after mixing a certain amount of L. extract, fatty acid, and fragrance having a ClogP value of 3 or more. As a result, it was confirmed that the residual concentration of the afterglow extract was higher than that in the case of using alone, when the fragrance of the fragrance of the fragrance extract, the fatty acid and the fragrance having the ClogP value of 3 or more was combined, and thus a high antimicrobial effect can be expected. In addition, the surprising fact is that the use feeling is also excellent due to the emollient action and the reverberation effect by the fatty acid in the oil phase.

Preparation example: Preparation of extract

The dried skins were pulverized and subjected to CO ₂ supercritical extraction at a temperature of 30 to 60 ° C and a pressure of 10 to 30 mpa. The extract was vacuum dried and used.

EXPERIMENTAL EXAMPLE 1 Evaluation of Antimicrobial Activity of Various Ingredients

The present inventors conducted a primary evaluation of antimicrobial effects against major skin uptake bacteria, odoriferous and acne bacterium by using paper disc method (about 100 kinds of bactericides known to have antimicrobial activity in the field of household products and cosmetics). In order to evaluate the antimicrobial activity against the species with excellent antimicrobial activity, the minimum inhibitory concentration (MIC) of each strain was measured using the two fold dilution method And the results are shown in Table 1 (unit: ppm). Test strains were Staphylococcus epidermidis , Corynebacterium xerosis , and Propionibacterium acnes , which are causative bacteria of the skin, . In the MIC measurement method, an antibacterial sample is diluted at a predetermined concentration interval in a liquid medium or added at a constant concentration interval in a sterilized solid medium, and then solidified. Then, the pre-cultured test liquid is inoculated into a liquid medium or one platinum is taken on a solid medium After culturing, the cells were incubated at 37 ° C for 24 hours.

Test substance S. epidermidis C.xerosis Propionibacterium acnes Acacia extract 0.1 0.5 6.0 Triclosan 0.1 1.0 7.8 Trichlorocavan 5.0 8.0 39.0 Xanthorrhizole 8.0 3.0 39.0 Berber 30.8 3.0 78.0 Tea trio day 62.5 7.8 625.0 Zincidone 62.5 78.0 625.0 Cooper gluconate 500.0 78.0 625.0 Bisabolol 500.0 625.0 6250.0 Gosam extract 500.0 78.0 500.0 Chitin 500.0 625.0 7813.0

As a result of the test, the extracts showed excellent antibacterial activity as a whole. Triclosan, trichlorocarban, berberin or xanthorrhizol showed excellent antibacterial activity. In particular, Corynebacterium xerosis , which is known to be found in many offensive odor owners who stimulate nose, showed excellent antibacterial activity against triclosan, and this strain was expected to have specificity.

Production of Examples 1 to 6 and Comparative Examples 1 to 4

According to the ingredients and contents shown in Table 2 below, the extract and the fatty acid were first dissolved in a perfume solution having a ClogP value of 3 or more. When the formulation was in a liquid state, the composition was placed in a human body washing substrate and then subjected to general mechanical or homomixer The soap base (Soap Noodles) was mixed with a liquid additive using a conventional soap making apparatus and then subjected to a molding and extrusion process so as to be extruded. In Example 1 To 6 and Comparative Examples 1 to 4 were prepared.

The above-mentioned human washing substrate used was a washing base in which an appropriate amount of at least one of a higher fatty acid alkali salt, a surfactant, a wetting agent, a sequestering agent, and purified water was mixed in an appropriate amount.

unit:
weight% Solid cosmetic soap composition Liquid type human body cleaning composition Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Example 4 Example 5 Example 6 Comparative Example 3 Comparative Example 4 Soap Noodles
(manufacturer:
Wilmar) To 100 To 100 To 100 To 100 To 100 - - - - - Potassium laurate / myristate - - - - - 5 10 20 5 10 Cocamidopropyl betaine - - - - - 25 20 10 25 20 glycerin - - - - - 5 10 15 5 10 When tetrasodium is added to the dithia - - - - - 0.5 0.3 0.2 0.5 0.3 Purified water - - - - - To100 To 100 To 100 To 100 To 100 Acacia extract 0.05 0.2 0.5 0.005 0.2 0.03 0.1 1.0 0.003 0.1 Palm oil / palm kernel retention defense (Manufacturer: Pacific Oleo) 1.0 5.0 20.0 5.0 - 1.0 2.0 30.0 0.5 - Spices
(ClogP 3.0 or later) 0.5 2.0 3.0 0.5 2.0 0.5 2.0 3.0 0.5 2.0

Experimental Example 2: Measurement of adsorption amount of extract

In order to evaluate the skin adsorption amount of the afterglow extract on the human body cleansing compositions of Examples 1 to 6 and Comparative Examples 1 to 4, distilled water was added to the human body cleansing composition previously prepared to prepare a 10% concentration solution. First, skin of the same area was secured from a hairless mouse (5 to 7 weeks old, male), hair was removed using a sharp knife with the stratum corneum facing upward in an adsorption cell, lt; / RTI > shaker. 30 g of the test substance was applied to the upper portion of the adsorption cell, and then the saker was operated at a rate of 120 rpm for 30 seconds to cause physical contact with the test substance and the skin. At this time, the area of contact skin is 10 cm ² . The adsorption cell was immediately tilted so that the test material was immediately followed and the cell was inverted for 30 seconds to remove the fluidized bed. 50 g of purified water at 25 캜 was added and shaken for 30 seconds at a rate of 120 rpm. The adsorbed cells were tilted to remove the fluidized bed as well. This washing procedure was repeated 5 times in the same manner. After removing the skin, the skin was cut to the same size using Biopsy Punch with a diameter of 10 mm, 5 pieces were put into the test tube, 1 g of ethanol was added and allowed to stand for 24 hours for vortexing ), Filtering, and quantitative analysis of L. extract was carried out by liquid chromatography. The hairless mice used were 3 mice per sample, and their average values were obtained. The adsorption amount (ug / cm ² ) per unit area and the skin adsorption rate of the following formula 2 were determined. The results are shown in Table 3.

Unit: Adsorption amount ug / cm ² Solid cosmetic soap composition Liquid type human body cleaning composition Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Example 4 Example 5 Example 6 Comparative Example 3 Comparative Example 4 Initial adsorption amount 17.5 40.5 53.7 1.5 36.5 11.4 25.5 71.5 10.9 22.5 After one wash, the adsorption amount (skin adsorption rate) 12.0
(68.6%) 28.9
(71.4%) 35.2
(65.5%) 0.8
(53.3%) 17.5
(47.9%) 7.5
(65.8%) 18.9
(74.1%) 51.2
(71.6%) 6.1
(56.0%) 10.9
(48.4%) The adsorption amount after 5 washing (skin adsorption rate) 10.0
(57.1%) 23.2
(57.3%) 29.3
(54.6%) 0.6
(40.0%) 13.7
(37.5%) 6.3
(55.3%) 15.2
(59.6) 39.5
(55.2%) 4.4
(40.4%) 8.7
(38.7%)

The results are shown in Examples 1 to 6, in which the extract of L. extract, the fatty acid, and the fragrance of more than ClogP 3 were mixed at a weight ratio of 1: 10: 5 to 1:50:25, Respectively.

Experimental Example 3: Clinical experiment for inhibition of odor and microorganism

The compositions for human body cleansing of Examples 2 and 5 and Comparative Examples 2 and 4 were directly applied to the underarms, and the degree of antibacterial and deodorization was evaluated. The number of microorganisms collected from the armpit was measured using an easy stamp. The degree of deodorization was evaluated by directly taking the odor from the armpit. The details of each are as follows.

(Evaluator Selection and Testing Protocol)

Twenty adults aged 20 or older who usually feel sweating or underarm odor as subjective or extrinsic are treated with the usual restriction deodorant, except that the armpit is cleaned with a soap provided for a certain period before the start of the test and during the test period. Cosmetics, fragrances and other products that could affect the test were not included in the evaluation site. Bacteria and odor of armpit were measured before starting the evaluation and used as a baseline after use of the product. Evaluation period was 2 weeks, and the left and right sides were symmetrically used in Examples 2 and 5 on the one hand, and Comparative Examples 2 and 4 on the other hand, respectively. The use was carried out once every day during the test period, and the shower was performed at the same time. After 4 hours, the microbial measurement and the odor evaluation were carried out once a week (the number of microbial bacteria in the armpit was measured).

The number of microorganisms was measured by directly sampling from the armpit using Easy Stamp TPC (Komed, Korea). The method of use is as follows: 1) After folding one by one so that the lid is not peeled off, 2) record the name and date of the subject, 3) open the lid of the easy stamp and lightly press the concave part of the armpit, Was incubated at 37 ° C for 24 hours or more. As a result, the number of colonies formed according to the method shown in Table 4 was determined as 0-5 according to the scoring scale, and the average value was obtained by comparing with the number of colonies measured before the test.

score Evaluation standard 0
One
2
3
4
5 less than 30 colonies (less than 30)
30-300 colonies (30-300 colonies)
> 300 colonies. Colonies distinct (more than 300 colonies)
Some but all colonies fused (not part of colonies)
Growth not completely confluent (only part of colonies are distinguished)
Confluent growth (no colonies)

The experimental results are shown in Table 5 below.

Solid cosmetic soap composition Liquid type human body cleaning composition Example 2 Comparative Example 2 Example 5 Comparative Example 4 Before use
(Baseline) 3.85 3.78 3.89 3.87 after use
(Reduction rate) 1.48
(62.9%) 2.32
(38.6%) 1.32
(66.1%) 2.35
(39.3%)

From the above results, it can be seen that the number of microorganism colonies in Examples 2 and 5 was significantly reduced compared with Comparative Examples 2 and 4.

Experimental Example 4: Measurement of odor intensity

Five well-trained sensory evaluation specialists performed a sniff test that directly took the intensity of the odor of the armpit. The evaluation was carried out in a constant temperature and humidity chamber to be ventilated, and the nose was directly smelled at a distance of 10 cm or less from the armpit. The results were compared with the pre-test measurement values at 0-5 points according to the scoring scale as shown in Table 6 below.

score Evaluation standard 0
One
2
3
4
5 No smell
The smell is very weak
Weak odor
The smell is usually
Strong smell
Strong smell

The experimental results are shown in Table 7 below.

Cosmetic soap composition Liquid type human body cleaning composition Example 2 Comparative Example 2 Example 5 Comparative Example 4 Before use
(Baseline) 3.95 3.89 3.82 3.87 after use
(Reduction rate) 1.57
(60.3%) 2.43
(37.5%) 1.49
(61.0%) 2.41
(37.7%)

From the above results, it can be seen that Examples 2 and 5 prepared according to the present invention have a remarkable effect of reducing body odor as compared with Comparative Examples 2 and 4.

As a result, the compositions for human body cleansing (Examples 2 and 5) of the present invention contained palm oil / palm kernel oil together with L. extract, compared with Comparative Examples 2 and 4 which did not contain palm oil / Palm core oil, It was confirmed that the suppression effect was remarkably excellent.

Experimental Example 5: Clinical trial for improving acne

The following experiments were conducted to evaluate the clinical effects of Examples 2 and 5 and Comparative Examples 1 and 3 on the prevention and treatment of acne.

Ten test subjects were selected for each of the 10 male test groups (used in Examples 2 and 5) and control groups (used for Comparative Examples 1 and 3) in the late 10th to late 20th test days, twice a day for 2 weeks The use of divided soaps was used to evaluate the prevention and improvement of acne. The evaluation method is to determine the degree of acne reduction according to the criteria described in Table 8 through visual observation after a certain grade according to the condition of acne before the start of the experiment, , It is judged that the effect is excellent. If the reduction is 1 to 2, the effect is moderate. If the decrease is not shown, the effect is no, and if the grade after the test is higher than the grade before the test, it is judged to be deteriorated.

score condition 0
One
2
3
4
5
6
7
8
9 Normal state
The number or size of cottonseed is very small or the inflammation does not proceed
The number and size of cottonseed are medium and the inflammation does not proceed
The presence of a small number of inflammatory papules
There is a small and broad nodule and inflammatory skin
There are small numbers and sizes of spherical nodules
There is a moderate inflammatory nodule
There is a large number of inflammatory nodules
There is a severe nodular appetite
There are severe cysts and inflammatory nodules

The experimental results are shown in Table 9 below.

Excellent effect Middle effect no effect Worse Skin side effects system Example 2 5 people 4 people 1 person 0 people 0 people 10 people Example 5 6 people 4 people 0 people 0 people 0 people 10 people Comparative Example 1 3 people 3 people 3 people 1 person 0 people 10 people Comparative Example 3 3 people 4 people 2 people 1 person 0 people 10 people

As shown in Table 9, the human body cleansing composition comprising 0.2% by weight (Example 2) or 0.1% by weight (Example 5) of the backbone extract contained 0.005% by weight (Comparative Example 1) or 0.003% (Comparative Example 3) was significantly superior to the human body cleansing composition.

Through the above four experiments, it was confirmed that the composition for human body cleaning comprising the fragrance of the present invention according to the present invention has a strong antimicrobial effect against body odor and acne bacterium. Also, it has been confirmed that the composition can be used in small quantities with a high degree of skin adsorption even after cleansing, has few side effects on the skin, and can effectively maintain and improve skin health by imparting an emollient effect.

Claims (12)

And a fragrance having a ClogP (octanol-water partition coefficient) value of 3 or more according to the following formula (1).
[Equation 1]

[2] The composition for human body cleaning according to claim 1, wherein the crude extract comprises 0.01 to 2% by weight based on the total weight of the composition. The composition according to claim 1, wherein the fatty acid is selected from the group consisting of C8-20 carboxylic acids including lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, and linoleic acid; Fatty acids derived from vegetable oils including palm oil, palm kernel oil, soybean oil, rape oil, corn oil, rapeseed oil, sunflower oil, safflower oil, cottonseed oil, sesame oil, rice bran oil, camellia oil, castor oil, olive oil and palm oil; Fatty acids derived from animal fats including tallow, lard, sunfish, fish oil, whale oil, and tuna oil; Fatty alcohols, mineral oils, and paraffins. ≪ Desc / Clms Page number 19 > The human body cleaning composition according to claim 1, wherein the fatty acid is contained in an amount of 1 to 30% by weight based on the total weight of the composition. The human body cleansing composition according to claim 1, wherein the fragrance having a ClogP value of 3 or more is contained in an amount of 0.1 to 5% by weight based on the total weight of the composition. [2] The composition for human body cleaning according to claim 1, wherein the composition comprises 0.01 to 2% by weight of an extract of Liliaceae and 1 to 30% by weight of a fatty acid based on the total weight of the composition. [Claim 2] The composition for human body cleansing according to claim 1, wherein the composition comprises a mixture of the backbone extract and the fatty acid in a weight ratio of 1:10 to 50 (backbone extract: fatty acid). The composition according to claim 1, wherein the composition is selected from the group consisting of rosemary extract, fatty acid, and fragrance having a ClogP (octanol-water partition coefficient) value of 3 or more in the ratio of 1: 10: 5 to 1: And 50:25 (afterglow extract: fatty acid: fragrance), based on the total weight of the composition.
[Equation 1]

The composition for human body cleaning according to claim 7 or 8, wherein the mixture comprises 1 to 35% by weight based on the total weight of the composition. The composition for human body cleaning according to claim 1, wherein the composition has antimicrobial activity against Corynebacterium xerosis and Propionibacterium acnes . In the preparation of a human body cleansing composition comprising an extract of Lilac,
A method for preparing a human body cleaning composition having an improved antimicrobial effect by increasing the skin adsorption rate of an extract of Lycoris sp. 12. The method according to claim 11, wherein the method comprises mixing the mixture of the backbone extract and the fatty acid in a weight ratio of 1:10 to 50 (backbone extract: fatty acid) and a mixture of ClogP (octanol-water partition coefficient, Octanol-water lt; RTI ID = 0.0 > 3, < / RTI >
[Equation 1]