KR20140141754A - Anti-microbial composition comprising extract from barks of alnus sibirica and use thereof - Google Patents

Abstract

The present invention relates to an anti-microbial composition against oral microbes comprising extract from barks of Alnus sibirica. The extract from barks of Alnus sibirica which is an active ingredient of the anti-microbial composition according to the present invention has an anti-microbial activity against oral microbes, especially against streptococcus mutans, thereby being useful as a composition for oral hygiene, a pharmaceutical composition, and the likes which prevents or treats streptococcus mutans related oral diseases such as molar, plaque inhibition, dental caries, periodontal disease, and the like.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an antimicrobial composition comprising a bark extract of Staphylococcus aureus and an antimicrobial composition comprising the same.

The invention teal tree (Alnus sibirica bark extract as an active ingredient.

Thorn tree ( Alnus sibirica ) is a member of the birch and ducks. It is known that over 17 species are native to Korea, and it is distributed in North Korea, China, and Japan. In the civilian and oriental medicine, the bark of the duck is called reddish, and it is used for the purpose of bleeding (鼻 hemorrhage), diarrhea and trauma due to the action of cleansing and strengthening. It is also shown that it is applied to the purpose of hanging out hangover. In addition, it is used as instrument material, civil engineering material, and floor, and bark and fruit are used as dyestuff, and it is planted widely for planting everywhere.

Diarylheptanoids such as oregonin and hitsutenone are known to be contained in the plants of the genus Alnus , and phenolic compounds such as flavonoid and tannin are also known. Recently, various physiological activities including diarylheptanoid compounds and antioxidative and anticancer effects have been reported.

On the other hand, the microorganisms distributed in the oral cavity were Lactobacillus acidophilus , Lactobacillus plantarum , Streptococcus mutans, Streptococcus sanguis , Streptococcus salivarius , Staphylococcus aureus , Staphylo - coocus epidermidis, and Enterobacter cloacae . These microorganisms may cause postnatal, tooth decay, and periodontal disease. In particular, Streptococcus mutans is known to be a major cause of dental caries. Dental caries is a serious illness that affects 80-90% of Koreans at least once a lifetime and is caused by the interaction of bacteria, food, and saliva in the placenta It is a disease in which the minerals in the teeth are demineralized and the dentin is destroyed, resulting in the defect of the dental tissue. Recently, the use of oral cleansers has been increasing for many people in order to eliminate the discomfort, in addition to the prevention of tooth decay. On the other hand, commercially available oral cleaning agents use synthetic compounds such as fluorine compounds, sodium hydrocarbons, light silicic anhydride, thymol, and zinc chloride as important components for sterilization and cleaning. Of course, these chemically synthesized compounds may be used in trace amounts and neglected in the short term for the harm to the human body, but they are reported to cause harm to the human body over a long period of use.

Therefore, in recent years, in order to solve the problems such as various side effects caused by the use of the chemical antibacterial agent, development of a composition for antibacterial use using a natural material extract has been the subject of major researches, 10-0387091), but it is still not yet.

The present invention has been conceived to solve the problems of the prior art as described above, teal tree (Alnus The present invention also provides an antimicrobial composition for oral microorganisms, which comprises a sibirica bark extract.

It is another object of the present invention to provide a composition for promoting oral hygiene which comprises extract of bark of Alnus sibirica .

It is yet another object of the present invention to provide a pharmaceutical composition for the treatment or prevention of oral diseases, which comprises extracts of a watery bark extract and a pharmaceutically acceptable carrier.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

The present invention provides an antimicrobial composition for an oral microorganism comprising an extract of a bark extract.

In one embodiment of the present invention, the oral microorganism is characterized by streptococcus mutans .

According to another embodiment of the present invention, the extract of bark of the thyme is contained in an amount of 1 wt% to 100 wt%.

In addition, the present invention provides a composition for promoting oral hygiene, which comprises an extract of a watery bark extract.

In one embodiment of the present invention, the extract of bark of Staphylococcus aureus is characterized by having an antimicrobial activity against Streptococcus mutans .

In another embodiment of the present invention, the composition for enhancing oral hygiene includes at least one additive selected from the group consisting of a carrier, an emulsifier, a flavoring agent, a flavoring agent, an antiseptic, a food coloring agent and a flavoring agent.

In another embodiment of the present invention, the composition for enhancing oral hygiene is characterized by having a formulation of a toothpaste, a mouthwash, a gum, a spray or an oral cleanser.

Furthermore, the present invention provides a pharmaceutical composition for the treatment or prevention of oral diseases, which comprises an extract of Staphylococcus aureus and a pharmaceutically acceptable carrier.

In one embodiment of the present invention, the oral disease is characterized by being a disease caused by streptococcus mutans .

The present invention relates to an antimicrobial composition for an oral microorganism containing extracts of a watery bark extract, wherein an extract of a watery bark extract, which is an effective ingredient of the antimicrobial composition according to the present invention, has antimicrobial activity against oral microorganisms, particularly streptococcus mutans The present invention relates to a method for preventing or treating oral diseases related to Streptococcus mutans , for example, prevention of tooth decay, plaque formation, tooth decay, periodontal disease, dental caries, etc., It is expected that the composition can be usefully used as a composition for promotion, a pharmaceutical composition and the like.

1 is a graph showing the bactericidal effect of Streptococcus mutans after the treatment of Sample 1 in the initial stage (A), (B) for 30 seconds, (C) for 1 minute and (D) It is a photograph which shows.
Fig. 2 is a graph showing the bactericidal effect of Streptococcus mutans after treatment of Sample 2 in the initial stage (A), (B) for 30 seconds, (C) for 1 minute and (D) It is a photograph which shows.
Fig. 3 is a graph showing the bactericidal effect of Streptococcus mutans after treatment of Sample 3 in the initial stage (A), (B) for 30 seconds, (C) for 1 minute and (D) It is a photograph which shows.
FIG. 4 is a graph showing the bactericidal effect of Streptococcus mutans after treating Sample 4 in the initial stage (A), (B) for 30 seconds, (C) for 1 minute and (D) It is a photograph which shows.
5 is a graph showing the effect of sterilization of Streptococcus mutans after the treatment of Sample 5 in the initial stage (A), (B) for 30 seconds, (C) for 1 minute and (D) It is a photograph which shows.

The inventors of the present invention have made efforts to discover an antimicrobial active material for oral microorganisms, particularly streptococcus mutans , from natural products. As a result, it has been found that the extract of Staphylococcus aureus is effective against Streptococcus mutans ( Streptococcus mutans ) And the present invention was completed on the basis thereof.

Hereinafter, the present invention will be described in detail.

The present invention provides an antimicrobial composition for an oral microorganism comprising an extract of a bark extract. In the present invention, the bark extract can be extracted by a conventional method known in the art for extracting an extract from a natural product, that is, using a conventional solvent under the conditions of ordinary temperature and pressure. For example, in the present invention, the watery bark extract is extracted using a solvent selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol can do. In addition, the method for extracting the extract from the bark of Japanese tholens can be extracted by various methods such as hot water extraction, cold extraction, reflux extraction, ultrasonic extraction and the like, but is not limited thereto.

The antimicrobial composition comprising the extract of bark extract according to the present invention has antibacterial activity on oral microorganisms, preferably streptococcus mutans .

In the antimicrobial composition according to the present invention, the content of the extract of the hardwood bark extract, which is an effective ingredient, may be contained in an amount sufficient to achieve antibacterial and germicidal effects, for example, 1 wt% (1,000 ppm) 2% by weight or more. Too low an extract of bark bark extract takes too long to achieve the sterilization target and is not efficient in bactericidal activity. Therefore, the content of the extract of the watery bark which is an active ingredient in the antimicrobial composition may be 1% by weight or more, preferably 2% by weight or more, and most preferably 2-3% by weight.

In one embodiment of the present invention, an extract of a watery bark extract (see Example 1) was prepared, an oral cleaning agent containing the same was prepared (see Example 2), and streptococcus mutans As a result, the antimicrobial composition containing the extract of bark extracts of the present invention has excellent antimicrobial activity against oral microorganisms, particularly Streptococcus mutans , and is useful as a composition for promoting oral hygiene or a pharmaceutical composition for treating or preventing oral diseases It can be very useful (see Example 3).

Accordingly, the present invention provides a composition for promoting oral hygiene comprising an extract of a watery bark, wherein the extract of the watery bark extract has an antibacterial activity against streptococcus mutans . The composition for enhancing oral hygiene according to the present invention may include, but is not limited to, dentifrice, toothpaste, gum, spray, mouthwash, mouthwash, and the like. In addition, the composition for enhancing oral hygiene according to the present invention may contain one or more additives selected from the group consisting of carriers, emulsifiers, flavors, flavoring agents, preservatives, food colors and flavoring agents, The present invention is not limited thereto, and those skilled in the art can appropriately select and combine them. For example, toothpaste which is one of the formulations of the composition for promoting oral hygiene according to the present invention contains an effective amount of a watery extract in paste carriers or ordinary powders composed of components including hydrophilic base, emulsifier, flavor, fragrance and preservative . Such toothpaste may contain an effective amount of an abrasive for mechanical destruction / removal of tartar and / or fluoride. In addition, a fluoride compound may be further used as a tooth decay agent. In order to prevent the discoloration of the active agent, Sodium m-bisulfite may be used. In addition, the oral cleansing agent may comprise an aqueous or aqueous-alcoholic liquid carrier, an antiseptic and, generally, from about 0.1% to about 50% by weight of the extract of aquatic tree bark.

The present invention also provides a pharmaceutical composition for the treatment or prevention of oral diseases, which comprises extracts of a watery bark extract and a pharmaceutically acceptable carrier. More specifically, the pharmaceutical composition can treat or prevent an oral disease caused by streptococcus mutans . The oral disease can be, for example, a posterior tooth, plaque formation inhibition, tooth decay, periodontal disease, dental caries But is not limited to this.

As used herein, the term "prevention" means any action that inhibits oral disease or delays the onset of the disease by administration of the pharmaceutical composition according to the present invention.

The term "treatment" as used in the present invention means any action that improves or alleviates symptoms caused by oral diseases by administration of the pharmaceutical composition according to the present invention.

The pharmaceutical composition according to the present invention may contain, in addition to the active ingredient, a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition according to the present invention includes, but is not limited to, saline, buffered saline, water, glycerol and ethanol, and may be formulated with a suitable preparation known in the art ), Mack Publishing Company, Easton PA).

The pharmaceutical composition according to the present invention may be formulated into a formulation such as a warning agent, a granule, a powder, a syrup, a liquid, a fluid, an oil, a suspension, a tablet, a capsule, a cream, . ≪ / RTI >

The pharmaceutical composition according to the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the intended method, and the dose may be determined depending on the condition and weight of the patient, The type of administration, the route of administration, and the time, but may be suitably selected by those skilled in the art.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dosage level is determined depending on the type of disease, severity, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition according to the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

Specifically, the effective amount of the pharmaceutical composition according to the present invention may vary depending on the age, sex, condition, body weight, absorbency, inactivation rate, excretion rate, type of disease, May be administered in an amount of 0.001 to 150 mg, preferably 0.01 to 100 mg, per 1 kg of body weight per day or every other day, or one to three times per day. However, the dosage may be varied depending on the route of administration, the severity of obesity, sex, weight, age, etc. Therefore, the dosage is not limited to the scope of the present invention by any means.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.

[Example]

EXAMPLES Example 1. Preparation of extracts of bark extract

Water wood (10 kg) was cooled in 80% acetone at room temperature for 3 days and then filtered. The extract was repeatedly extracted three times in total and concentrated under reduced pressure to obtain an extract (640 g).

Example 2: Production of natural mouthwash (ASG) containing extracts of bark extract

Five natural mouthwashes having different contents were prepared including the extracts of bark extracts of Example 1 as an active ingredient. More specifically, the extracts of Streptomyces bark were added to the sterilized distilled water of tertiary sterilized water at a temperature of 40 ° C for 20 minutes, sonicated with grapefruit seed extract, Jeju citrus extract, xylitol and menthol for 20 minutes . Then, centrifugation was carried out at 3000 rpm for 20 minutes, the paper filter was repeated 4-5 times, and finally filtered through a 0.45 μm nylon filter. Finally, pressurized steam sterilization was performed at 90 ° C for 10 minutes. Finally, limonene was added to make a final natural mouthwash (ASG). The specific content of the prepared natural mouthwash is shown in Table 1 below.

sample 1 sample 2 sample 3 sample 4 sample 5 Tertiary sterilization
Distilled water 400ml 400ml 400ml 400ml 400ml Bark extract 1 wt% 2 wt% 2 wt% 3 wt% 3 wt% Jeju Citrus
extract 5 wt% 5 wt% 5 wt% 5 wt% 5 wt% Grapefruit seed
extract 0.1 wt% 0.1 wt% 0.3 wt% 0.1 wt% 0.3 wt% menthol
(menthol) 0.15 wt% 0.15 wt% 0.15 wt% 0.15 wt% 0.15 wt% Xylitol
(xylitol) 2 wt% 2 wt% 2 wt% 2 wt% 2 wt% Limonene
(limonene) 0.01 wt% 0.01 wt% 0.01 wt% 0.01 wt% 0.01 wt%

Example  3. Streptococcus Mutans ( streptococcus mutans )on  Verification of antimicrobial activity against

The samples 1 to 5 prepared in Example 2 were used as test solutions and the antibacterial activity test for Streptococcus mutans was performed as follows.

That is, the test strain Streptococcus mutans was inoculated into Brain heart infusion (BHI) and cultured at (37 ± 1 ° C.) for (24 ± 2) hours. The culture solution was diluted, 1 to 9 × 10 5 CFU / mL. When the test strain was added to 20 mL of the test solution (sample stock solution), and the mixture was allowed to stand at room temperature for 30 seconds, 1 minute, and 2 minutes, the number of bacteria was measured to calculate the bacterial reduction rate to the initial number of bacteria. The results are shown in Table 2, and the photographs showing the sterilization effect of each sample with time are shown in Figs. 1 to 5. At this time, the number of bacteria was measured immediately after the test strain was inoculated using sterile physiological saline, and the number of bacteria was indicated as the initial number of bacteria. In the initial dilution stage of all experiments, neutralization was carried out using D / E neutralization broth. When bacteria were grown in the medium, the number of bacteria on the medium was multiplied by the dilution factor to calculate the final number of bacteria. In the case where the microorganism did not grow in the medium, it was multiplied by the dilution factor obtained in the neutralization step and expressed as "less than 10 (<10)". Bacterial counts at all stages were measured using Tryptic soy agar (TSA), and the number of viable cells was calculated according to the following equation (1), and the rate of bacterial reduction was determined according to equation (2) ].

[Equation 1]

N = C x D

Here, N is the number of living cells, C is the number of colonies (average number of two colonies), and D is the dilution factor (dilution factor of the diluent).

&Quot; (2) &quot;

R (%) = [(A - B) / A x 100]

Here, R represents the bacterial reduction rate, A represents the number of initial bacteria, and B represents the number of bacteria after a certain time.

As shown in Table 2 and Figs. 1 to 5, it was confirmed that more than 80% of Streptococcus mutans was removed 2 minutes after the treatment of Sample 1 to Sample 5, , Respectively. From the above results, the antimicrobial composition comprising the extract of the bark of Staphylococcus aureus has excellent antimicrobial activity against oral microorganisms, particularly Streptococcus mutans , and can be used as a composition for promoting oral hygiene or a pharmaceutical composition for treating or preventing oral diseases It can be very useful.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.

Claims (9)

An antimicrobial composition for oral microorganisms, comprising extracts of bark extracts.
The antimicrobial composition according to claim 1, wherein the oral microorganism is streptococcus mutans .
The antimicrobial composition according to any one of claims 1 to 3, wherein the extract is 1% to 100% by weight of the extract.
A composition for enhancing oral hygiene comprising extracts of a bark extract.
[Claim 5] The composition for promoting oral hygiene according to claim 4, wherein the extract of Staphylococcus aureus has antibacterial activity against Streptococcus mutans .
5. The oral hygiene promoting composition according to claim 4, wherein the composition for enhancing oral hygiene comprises at least one additive selected from the group consisting of a carrier, an emulsifier, a flavoring agent, a flavoring agent, an antiseptic agent, a food coloring agent, / RTI &gt;
5. The composition for enhancing oral hygiene according to claim 4, wherein the composition for enhancing oral hygiene comprises a formulation of a toothpaste, a mouthwash, a gum, a spray or an oral cleanser
A pharmaceutical composition for the treatment or prevention of an oral disease, comprising a watery bark extract and a pharmaceutically acceptable carrier.
9. The pharmaceutical composition according to claim 8, wherein the oral disease is a disease caused by streptococcus mutans .

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