KR20140101520A - A pharmaceutical composition for improving generative of male and treating infertility - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for improving a male sexual function and treating sterility, and more specifically, to a pharmaceutical composition for improving a male sexual function and treating sterility, wherein the composition contains, as an active ingredient, at least one extract selected from a Cornus officinalis extract, a Chinese matrimony vine extract, a Cuscuta japonica extract, a Schisandra chinensis extract, and a Plantago asiatica extract; and to a food composition for improving the male sexual function and treating sterility. The composition has an effect in improving the male sexual function and treating sterility through NO concentration, an angiotensin converting enzyme (ACE) inhibitory effect, a 5-phosphodiesterase (PDE) inhibitory effect, and the CREM expression in HUVEC, and an increase in the number of sperms, an enhancement in sperm activity, an increase in testosterone content, and the like.

Description

Technical Field [0001] The present invention relates to a pharmaceutical composition for improving male sexual function and treating infertility,

The present invention relates to a pharmaceutical composition for improving male sexual function and treating infertility. More particularly, the present invention relates to a pharmaceutical composition for treating male sexual dysfunction and infertility. More particularly, the present invention relates to a pharmaceutical composition for treating male sexual dysfunction and infertility comprising at least one extract selected from the group consisting of corn oil extract, A pharmaceutical composition for the treatment of male sexual dysfunction and infertility, and a food composition for improving male sexual function and preventing infertility. The composition can improve male sexual function through HUVEC, NO concentration, ACE (Angiotensin converting enzyme) inhibitory effect, PDE (Phosphodiesterase) -5 inhibitory effect, CREM expression and the like, sperm count, sperm activity and testosterone content It is effective for infertility.

The modern living environment is confused by overeating, drinking, stress, environmental pollution, etc., and the frequency of exposure to lifestyle diseases such as hypertension, diabetes and obesity increases, , Aging, and sexual dysfunction. In the case of people aged 40 years or older, it is more severe. Infertility, especially during poor sexual functioning, is a social problem. It is important to solve the problem of erectile dysfunction through improvement of blood flow, but more importantly, it is more important to solve the infertility by improvement of sex hormone, improvement of sperm count and activity.

Infertility is the inability to conceive without contraception and a normal couple living for more than a year. Infertile couples account for 10 to 20 percent of all couples. The incidence of infertility in the United States is about 15% of normal infertility and in Korea, 8% of outpatients in obstetrics and gynecology are infertility. The cause of infertility is about 40% in women only, 40% in men only, about 10% in both males and females, and about 10% in unexplained cases. Half of them are male factors (Korean Society for Sexual Medicine and Andrology, Namseonggwahak, Seoul, Gunjachulpansa, 5-54, 161-163 (2003), Korean Urological Association, Textbook of Urology, 3rd Seoul Goryeouihak 507 -22 (2001)).

Male infertility leads to infertility if any part of the sperm is produced and transported and then released by ejaculation and then combined with the oocyte. In modern men, sperm production and status, spermatogenesis and sperm motility this has been the most problematic part of the (Jouannet P, et. al. , APMIS. 109 (3), 333-44. (2001)). The spermatogenesis process is the most common among male infertility, which is mainly due to hormonal secretion to the hypothalamus, pituitary gland, testis, genetic factors, testicular dysfunction, latent testis, varicose veins, exposure to high temperatures (Spermatogenesis), which is the cause of spermatogenesis, is not normally caused by the formation of spermatozoa, or the formation rate of deformed spermatozoa is high (The Korean Urological Association, Textbook of Urology, 3rd, Seoul Goryeouihak, 507-22. Sperm motility is an essential element in the process of combining with fertilization (Tienthai P. Anim Reprod Sci . 80, 131-46. (2004)). The motility of sperm begins to acquire fertility through the uterus of a woman, leading to hyperactivation and acrosome reaction (Guzick DS et al., N Engl J Med . 345, 1388-93 (2001).

Recently, male infertility has been increasing due to frequent drinking, smoking, environmental pollution, intake of nutrients, excessive use of medication, exposure to environmental toxins, excessive sexual intercourse, fatigue and stress. As a result, there can appear such as a decrease (Saleh RA and Agarwal A. J Androl . 23, 737-52. (2002)).

The causes of male infertility are amniotic fluid, impaired sperm quality, impaired spermatogenesis, and decreased motility of the sperm. (Korean Journal of Urology, 2001. Seoul Goryeouihak 507-22. Spermatogenesis disorder has a feeling jajeung (oligozoospermia), a weak arrangements jajeung (asthenozoospermia) activity of the sperm to be made less then the number of sperm produced in the testes (Saleh RA and Agarwal A. J Androl . 23, 737-52. ( 2002).

Spermatozoa can be spermatozoa, and the spermatozoa of the spermatozoa can be spermatozoa. The spermatozoa can be spermatozoa, And the amount of sperm should be 2 ~ 5 ㎖, sperm deficiency, sperm 1 ㎖ of sperm count is less than 30 million is the cause of infertility.

A variety of studies on compositions for the prevention or treatment of male reproductive dysfunction have been patented or registered. In the prior art, cadmium chloride is orally administered to experimental animals to cause artificial sperm damage to induce male reproductive dysfunction (Japanese Patent Application Laid-Open No. 2002-0085401), or tetrachlorodibenzo-p-dioxin is treated (Korean Patent No. 10-0508539). However, efficacy research studies for prevention or treatment of all male reproductive dysfunctions were not rational as they did not consider the actual production period of sperm. For example, Korean Patent No. 10-0508539, which improves male reproductive function, does not disclose detailed experimental methods in the method of searching for efficacy using an experimental animal. In the case of prevention or treatment of male reproductive dysfunction, In the composition for promoting and the method for producing the same (Patent Document No. 2002-0085401), the testes were extracted one week or two weeks after the oral administration of the liquid to the experimental animals, and the sperm count and sperm activity were observed. In addition, in the composition for preventing and treating male reproductive dysfunction (Laid-open Patent Publication No. 10-2005-0028475), the composition was intraperitoneally administered twice a day for 18 days to the experimental animals, Activity and duration of sperm activity.

Research on male infertility is largely based on western medical treatments, and research on herbal medicines using herbal medicines has been left behind. However, there are many successful cases in the treatment of sexual dysfunction in Korea, depending on the ethical and socioeconomic reasons. In Korea, there have been a lot of successful cases. However, Most of them are based on the literature and there is no systematic study of precise mechanism and each prescription. Most of the health foods that are currently sold are mostly produced by ignoring scientific studies such as proper amount of effective ingredients, synergistic effect, and duration of taking and mixing the ingredients known to be good for sexual function improvement.

Accordingly, the present inventors have made efforts to develop a therapeutic agent for male infertility, wherein at least one extract selected from the group consisting of corn oil extract, Gugija extract, bokbunja extract, Tosan extract, Omiza extract and tea extract has cytotoxicity in TM3 cells, In vitro tests such as concentration, inhibition of angiotensin converting enzyme (ACE), inhibition of PDE (Phosphodiesterase) -5 and CREM expression, changes of cGMP in serum, changes of testosterone in serum and organ weights (testis, The present invention has been accomplished by confirming that male sterility improvement and infertility treatment are effective through in vivo test of measurement, sperm count and sperm motility.

It is an object of the present invention to provide a pharmaceutical composition for improving male sexual function and treating infertility by using corn oil extract, Gugija extract, Bokbunja extract, Sorghum extract, Omiza extract and Tea extract.

It is another object of the present invention to provide a food composition for improving male sexual function and preventing infertility by using corn oil extract, Gugija extract, bokbunja extract, Sorghum extract, Omiza extract and Tea extract.

In order to achieve the above object, the present invention provides a pharmaceutical composition for improving male sexual function and treating infertility, which comprises at least one extract selected from the group consisting of corn oil extract, Gugija extract, bokbunja extract, Sorghum extract, Omiza extract, .

Also, the present invention relates to a method for improving male sexual function and a method for enhancing male sexual function comprising at least one kind of extract selected from the group consisting of a vegetarian oil-containing food additive, a corn oil extract, a Gugija extract, a bokbunja extract, a Sorghum extract, an Omija extract, To provide a food composition for preventing infertility.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for improving male sexual function and treating infertility, which comprises as an active ingredient at least one extract selected from the group consisting of corn oil extract, Gugija extract, bokbunja extract, Sorghum extract, Omiza extract and tea extract.

In the pharmaceutical composition for improving male sexual function and infertility of the present invention, it is preferable that the extract further comprises a pharmaceutically acceptable carrier. In this case, the extract of corn oil, gugija extract, bokbunja extract, (50 to 150 parts by weight), 20 to 100 parts by weight of brambles and 20 to 100 parts by weight of extracts (based on 100 parts by weight of the extract of corn oil) More preferably 10 to 50 parts by weight. Or the composition preferably contains a tea extract as an active ingredient.

In the pharmaceutical composition for improving male sexual function and infertility of the present invention, the extract may be prepared by: i) adding water or ethanol to the medicament and extracting it at 80 to 150 ° C for 1 to 4 hours; Ii) removing the impurities precipitated in the extraction solution by centrifugation at 1000 to 5000 rpm and filtering the filtered solution through a 0.3 to 0.6 mu m filter; And iii) concentrating the filtrate with a rotary vacuum concentrator.

Specifically, in the pharmaceutical composition for improving male sexual function and treating infertility of the present invention, 1 to 20 times of water is added to the above fermented sour milk, corn, And filtering, or 1 to 20 times 10 to 50% ethanol is added, and the mixture is extracted at 40 to 100 ° C for 1 to 24 hours and then filtered.

Among them, KH-204, which is composed of corn oil, Gugija, bokbunja, Tosa and Omiza extracts, was named ethanol extract and hot water extract.

Specifically, the extract can be obtained by extracting each medicinal substance with water or an organic solvent. Examples of the organic solvent include lower alcohol, acetone, chloroform, methylene chloride, ether, ethyl acetate, hexane and the like. As the lower alcohol, methanol, ethanol, propanol and butanol can be mentioned, and ethanol is most preferable.

Or water of 1 to 20 times, preferably 2 to 15 times, more preferably 10 times, to 80 to 150 占 폚, preferably 100 占 폚 At a temperature of 120 to 120 DEG C for 1 to 24 hours, preferably 3 to 5 hours, more preferably 4 hours, and then filtered to obtain a hot-water extract of each medicinal material. The resulting filtrate is concentrated under reduced pressure Can be manufactured. In the above extraction methods, the extraction process may be repeated twice or more as necessary, and the extract obtained after filtration may be lyophilized or dried under reduced pressure to a powder form.

Specifically, a 10 to 50% aqueous solution of ethanol is added to the dried vegetable oil, the egg yolk, the bokbunja, the tobacco, the oyster, and the chrysanthemum and the dried or powdered powders at 1 to 20 times, preferably 2 to 15 times, more preferably 10 times, Preferably at 50 to 60, for 1 to 24 hours, preferably 3 to 5 hours, more preferably 4 hours, and then filtered to produce an ethanol extract of each drug. And concentrating the filtrate under reduced pressure. In the above extraction methods, the extraction process may be repeated twice or more as necessary, and the extract obtained after filtration may be lyophilized or dried under reduced pressure to a powder form.

In addition, the pharmaceutical composition of the present invention may further comprise other herbal medicines or extracts thereof which are pharmaceutically acceptable for enhancing the pharmacological effect. In this case, an extract of a herbal medicine is prepared according to the above extraction method, and then the herbal medicine is added to the pharmaceutical composition, or one or more selected medicinal herbs selected from the group consisting of the above fermented milk, corn, gum, The obtained extract may be included in the composition. Pharmaceutically acceptable " as used herein refers to those which are physiologically acceptable and do not normally cause an allergic reaction or the like when administered to humans.

In addition, the pharmaceutical composition according to the present invention may further contain one or more pharmaceutically acceptable carriers, excipients or diluents.

The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration.

Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like.

In addition, the carrier for parenteral administration may contain water, a suitable oil, a saline solution, an aqueous glucose and a glycol, and may further contain a stabilizer and a preservative. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. Other pharmaceutically acceptable carriers can be found in Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, Pa., 1995).

The pharmaceutical composition for improving male sexual function and treating infertility of the present invention can be administered to mammals including humans by any method. For example, it can be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration Lt; / RTI > Preferably, the pharmaceutical composition of the present invention can be transdermally administered. Refers to administering the pharmaceutical composition of the present invention to the cells or skin to allow the active ingredient contained in the pharmaceutical composition for male sexual function and infertility treatment to be delivered into the skin. For example, the pharmaceutical composition of the present invention can be administered in a scanning type formulation, pricking the skin lightly with a 30 gauge needle, or directly applying it to the skin.

The pharmaceutical composition of the present invention may be formulated into oral or parenteral dosage forms according to the route of administration as described above.

In the case of a preparation for oral administration, the composition of the present invention may be formulated into a powder, a granule, a tablet, a pill, a sugar, a tablet, a liquid, a gel, a syrup, a slurry, . For example, an oral preparation can be obtained by combining the active ingredient with a solid excipient, then milling it, adding suitable auxiliaries, and then processing the mixture into a granular mixture. Examples of suitable excipients include, but are not limited to, sugars including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches including corn starch, wheat starch, rice starch and potato starch, Cellulose such as methylcellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose and the like, fillers such as gelatin, polyvinylpyrrolidone and the like. In addition, crosslinked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may optionally be added as a disintegrant. Further, the pharmaceutical composition of the present invention may further comprise an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent.

In the case of a preparation for parenteral administration, it can be formulated by a method known in the art in the form of injection, cream, lotion, external ointment, oil, moisturizer, gel, aerosol and nasal aspirate. These formulations are described in Remington's Pharmaceutical Science, 15th edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a commonly known formulary for all pharmaceutical chemistries.

The total effective amount of one or more extracts selected from the active ingredients of the composition of the present invention, which is an extract from corn oil, gugija extract, bokbunja extract, zoote extract, omija extract and tea extract, can be administered to a patient in a single dose , Or a fractionated treatment protocol administered over a prolonged period of time in multiple doses. In the pharmaceutical composition of the present invention, the content of the active ingredient may be varied depending on the degree of the disease. Preferably, the preferred total dose of the at least one extract selected from the group consisting of the extract of corn oil, gugija extract, bokbunja extract, gum extract, omija extract and tea gum extract is about 100 ㎍ to 5 mg per kg body weight per day Lt; / RTI > to 1 mg. However, the dose of the extract may be determined based on various factors such as age, body weight, health condition, sex, severity of disease, diet and excretion rate of the patient as well as administration route and frequency of treatment of the pharmaceutical composition, In view of this, one of ordinary skill in the art will be able to determine the appropriate effective dose of the extract according to the specific use as male sexual function improvement and infertility treatment. The pharmaceutical composition according to the present invention is not particularly limited to its formulation, administration route and administration method as long as the effect of the present invention is exhibited.

In the present invention, the term 'male sexual function improvement and infertility treatment' refers to normalization or enhancement of sperm count, sperm activity, testosterone hormone, sexual behavior and sexual function, which can usually cause infertility.

Also, the present invention relates to a method for improving male sexual function and a method for enhancing male sexual function comprising at least one extract selected from the group consisting of a vegetarian oil-soluble food additive, a corn oil extract, a coriander extract, a bokbunja extract, To provide a food composition for preventing infertility.

The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food and food additives. Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art.

For example, the health food may be prepared by preparing the corn oil extract of the present invention, Gugija extract, bokbunja extract, Sorghum extract, Omija extract and tea extract as a tea, juice and drink in the form of tea, juice and drink, or granulating, It can be taken in powder form. In addition, the composition of the present invention can be prepared in the form of a composition by mixing it with a known substance or an active ingredient known to have male fertility improving effect and infertility prevention effect with the extract of corn oil, Gugija extract, bokbunja extract, Sorghum extract, Omija extract, .

Functional foods also include beverages (including alcoholic beverages), fruits and their processed foods (e.g., canned fruits, bottled, jam, maalmalade, etc.), fish, meat and processed foods such as ham, Etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, The extract may be prepared by adding the edible oil of the present invention such as Ganoderma lucidum extract, Gumijia extract, Crassulaceae extract, Sorghum extract, Omija extract and Tea extract to various kinds of seasonings such as retort food, frozen food and various seasonings such as soybean paste, soy sauce and sauce.

In addition, in order to use the extract of corn oil, gugija extract, bokbunja extract, gosan extract, omija extract and tea extract of the present invention in the form of food additives, it may be used in the form of powder or concentrate.

Sansui is a dry leaf of the fruit of the mountain fruit which is a deciduous tree belonging to the Tertiary tree family, and it is tasted and has a warm characteristic. According to the "Dongbibogam" and the "Fragments of the Fugue", it has the effects of strengthening the 阴 阴, 肾 精 and 肾气, and converging the head, tinnitus, sea water, It is said that it can be effective even in the case of perspiration. The most widely known Chinese medicines for the general public with nourishing medicines are Xuzen Daibôtang and Jukmi 황 黄 汤, which are the most important medicines in this prescription.

Gugija is a deciduous shrub with a height of 2.5 m, the ends of the branches are hanging down, the branches are spiny, and the leaves are 2 to 3 short-lived in the bottom part of the long branch. From time immemorial, Donguibogam has been known as "sexual cold", "migo", "nontoxic", dominating the dynasty of internal injustice and insomnia, strengthening the muscles, It strengthens the Yin and reigns the Ono and the Seven Wounds, and regularly watches and changes the complexion to whiten, to honor, to lust, and to longevity. " In addition, there is no toxicity in the main gangbang, it can cool the heat, it accumulates in the body, inflammation of the chest, noise, diabetes, joints, rheumatism, neuralgia is good and long time to take the muscle healthy, refreshing body, It has been used for a long time by various methods such as tea and alcohol.

Bokbunja is a deciduous shrub belonging to the Rosaceae. It is a dried fruit of unripe bokbunja berries and has a warm and tasty character. It is effective in protecting the liver and kidneys. According to Donguibogam, the bokbunja has a temperamental character, sweet taste, and no poison. "I take care of the ugliness of the man's nephrol and the innocent of the woman, strengthening and lengthening the man's tongue, , Clears the eyes, makes the kidneys full of energy, lightens the body, and keeps hair from whitening. " In addition, recent studies have demonstrated the efficacy of clearing kidney function, infertility, moxibustion, lipid peroxidation, tonic and blood, clearing the liver and clearing the eyes, and as a hydrolyzable tannin of berry berries, Gallotannin and 3 kinds of ellagitannin were separated from each other. Based on physicochemical properties and spectral data, galactic acid, 2,3- (S) -HHDP-D-glucopyranose, sanguinin, H-4 and H-6. Bokbunja showed strong inhibitory activity against enzymatic lipid and oxidation, and phenolic compounds such as gallic acid were identified as a component thereof. Phenolic compounds are one of the secondary metabolites widely distributed in food systems. They have various structures and molecular weights. They have a phenolic hydroxyl group and thus have a property of binding to macromolecules such as proteins. , Antibacterial activity, and nitrite scavenging ability.

The tosaja is the dried seed of Simsangsham and equispecific plant which is the first year parasitic herbaceous belonging to the Confucianism. It has a tasty, sweet and sweet character and is effective in protecting against cancer, hypertension, hypotensive action, hypoglycemia, kidney and spleen. In Dong-bo-bo, he said, "It strengthens the energetic and energetic effect. It is effective for symptoms of low back pain and knee. If you drink it from time to time, it will be effective for diabetes." If you take it for a long time, your eyes will brighten and you can live longer. "

Omija is a dried mature fruit of Magnolia and Magnolia, and has five flavors. It has the effect of acting on liver function, anti-aging, antibacterial, metabolism and immune function. Donguibogam protects the lungs and kidneys, and heal (虚 勞), ejaculation, burning, and resolves the problem is said. Such Omiza is widely used as tea, liquor, and lacquer.

Tea is a seed of Plantago asiatica L. which is a plant belonging to Plantaginaceae. It is a kind of plant which has the effect of Ishizu, Qingyu, Nomi, And it is mainly used to treat urinary incontinence, epilepsy, hematuria, seawater and intentional (redness). According to <Dongbu Bohak>, Chae-ee is weak in energy and mainly treats that urine does not come out well, controls five kinds of gonorrhea, makes urine clogged, works well, excretes moisture well, And it is said that it controls the openness of the soy sauce. Therefore, secondary electronegal swelling due to the swelling of the type of poison is improved.

The present inventors measured the cytotoxicity of TM3 cells, which are testicular cells of male mice, by MTT assay using hydrothermal and 30% ethanol extracts of each of corn oil, goji, bokbunja, (See Table 1 and Figs. 1 to 8). As a result, it was confirmed that there was no toxicity in KH-204 ethanol, KH-204 hydrothermal solution, acid fermented milk, Omija, and Gugija, and the toxin samples were slightly toxic.

In addition, HUVEC cells were used to measure eNOS production and the effect of NO production closely related to erection (see Table 3 and Figs. 9 and 10). As a result, it was confirmed that the KH-204 30% ethanol extract and hot water extract group increased nitrate concentration. That is, the samples improve the function of the penis by increasing the production of NO, a vasodilator.

In addition, ACE (angiotensin converting enzyme) inhibitory effect of each extract of the present invention was measured (see Table 5 and FIG. 11). The results were as follows: Gugija (49.1%), KH-204 hot water extraction (47.0%), Omija (45.9%), Cha Electronics (36.3%), KH-204 30% Ethanol Extraction (34.2% , Respectively. Therefore, these samples help to maintain the erection by increasing the blood flow of the penis indirectly through relaxation of the blood vessel.

In addition, PDE (Phosphodiesterase) -5 inhibition was measured (see Table 8 and Fig. 14). The PDE-5 inhibitory effect was in the order of bokbunja (34.5%), omija (35.6%), gujisi (32.8%) and KH-204 (30% ethanol extraction, 25.6%).

We also measured the organ weights and sperm function using animal experiments. The KH-204 ethanol extract and the positive control group, red ginseng, showed that the weight of the mice (see Table 10 and FIG. 15) was lower than that of red ginseng. However, Jungnam weight tended to increase with higher KH-204 (ethanol extract) concentrations (see Table 11 and Figure 16). The testimony weights were similar between the group administered with KH-204 (ethanol extract) and the group administered with red ginseng (see Table 12 and FIG. 17). The weights of the adrenal glands were similar to those of KH-204 (ethanol extract) and red ginseng groups (see Table 13 and FIG. 18). Sperm count tended to increase with increasing concentrations of KH-204 (ethanol extract), and the number of spermatozoa was higher in all groups treated with KH-204 (ethanol extract) than the 400 red ginseng group used as a positive control group And Fig. 19). Sperm motility of the mice was also increased with increasing concentration of KH-204 (ethanol extract), and higher sperm motility was observed in all groups treated with KH-204 (ethanol extract) than the 400 group of red ginseng used as a positive control (See Table 15 and Figs. 20 to 26).

The present invention, which is constituted as described above, is a pharmaceutical composition for improving male sexual function and treating infertility and has an effect of increasing sperm count, motility of sperm, content of testosterone hormone, sexual behavior, sexual function, Can help improve male reproductive function and sexual function as well as patients suffering from sexual dysfunction and male infertility without adverse side effects and ethical and social problems.

FIG. 1 is a graph showing the cytotoxicity confirmation experiment of the KH-204 ethanol extract. FIG.
FIG. 2 is a graph showing an experiment for confirming cytotoxicity of the hot-water extract of KH-204. FIG.
3 is a graph showing an experiment for confirming cytotoxicity of the extract of corn oil.
4 is a graph showing an experiment for confirming cytotoxicity of the extract of Omija.
5 is a graph showing an experiment for confirming cytotoxicity of the bokbunja extract.
FIG. 6 is a graph showing an experiment for confirming the cytotoxicity of the extract of the soil.
FIG. 7 is a graph showing an experiment for confirming cytotoxicity of a tea extract. FIG.
8 is a graph showing an experiment for confirming the cytotoxicity of Gujuga extract.
FIG. 9 is a graph showing the change of nitrate in HUVEC of KH-204 ethanol extract. FIG.
10 is a graph showing changes in nitrate in HUVEC of KH-204 hot water extract.
FIG. 11 is a graph showing the results (%) of the ACE inhibition rate of each sample. The inhibition rate of angiotensin-converting enzyme was shown for each sample (concentration: 500 ppm).
12 is a graph illustrating the principle of measuring the PDE (Phosphodiesterase) -5 inhibitory effect used in the present invention.
13 and 14 are graphs showing a standard curve (FIG. 13) for obtaining PDE-5 inhibition rate (%) and a measurement result of Phosphodiesterase-5 inhibition rate (FIG. 14) for each sample (500 ppm).
FIG. 15 is a graph showing the final weight of a mouse after oral administration of KH-204 30% ethanol extract sample by concentration for 8 days.
FIG. 16 is a graph showing the left side seminal vesicle weight after oral administration of KH-204 30% ethanol extract sample by concentration for 8 days.
17 is a graph showing the weight of the left testis of a mouse after oral administration of KH-204 30% ethanol extract sample for 8 days by concentration.
18 is a graph showing the left occipital weights after oral administration of a 30% ethanol extract of KH-204 according to concentration for 8 days.
19 is a graph showing the number of mouse sperm after oral administration of KH-204 30% ethanol extract sample for 8 days by concentration.
20 is a graph showing mouse sperm motility (%) after oral administration of KH-204 30% ethanol extract sample by concentration for 8 days.
21 to 26 are photomicrographs showing the motility of sperm. Sperm photographs (optical microscope 200 magnification) (FIG. 21) of the control group and sperm photographs of the group administered with KH-204 30% ethanol extract 50 mg / (Optical microscope 200 magnification) (FIG. 22), a sperm photograph (optical microscope 200 magnification) (FIG. 23) of a group in which KH-204 30% ethanol extract 100 mg / kg was administered, KH-204 30% ethanol extract 200 mg / (Photomicroscope 200 magnification) (FIG. 24) of a group of mice administered with a 30% ethanol extract of KH-204 (FIG. 25) and a positive control group , And a sperm photograph (optical microscope 200 magnification) of a group administered with 400 mg / kg of red ginseng.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

&Lt; Preparation Example > Preparation of composition for male sexual function improvement and infertility treatment

<Preparation Example 1> Preparation of 30% ethanol extract

<1-1> Preparation of 30% Ethanol Extract of Sansui Oil

First, impurities were removed, and 22 g of well dried soybean oil was prepared, and 4 to 8 times 30% aqueous ethanol solution was added thereto, followed by reflux extraction at 60 to 70 for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 or less.

<1-2> Preparation of 30% Ethanol Extract of Gugija

The impurities were removed and well dried gugija (42 g) was prepared. A 4- to 8-fold aqueous 30% ethanol solution was added and the mixture was refluxed at 60 to 70 ° C for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less.

<1-3> Preparation of 30% Ethanol Extract of Bokbunja

The impurities were removed and 26 g of well-dried brambles were prepared. A 4- to 8-fold aqueous 30% ethanol solution was added and the mixture was refluxed at 60 to 70 ° C for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less.

<1-4> Preparation of 30% Ethanol Extract of Soja

The impurities were removed and well dried to obtain 26 g of a soil material. A 4- to 8-fold aqueous 30% ethanol solution was added thereto and the mixture was refluxed at 60 to 70 ° C for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less.

<1-5> Preparation of 30% ethanol extract of Omiza

The impurities were removed, and well dried 6 g of Omija was prepared. 4 to 8 times of 30% ethanol aqueous solution was added and the mixture was refluxed at 60 to 70 ° C for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less.

<1-6> Preparation of KH-204 30% Ethanol Extract

The concentrate was prepared as powder, and a 10-fold amount of purified water was added thereto. The solution was boiled for 2 hours in a hot water tank (Daewoong, Korea), and the solution was centrifuged at 3,000 rpm. (ILSHIN, Korea) to recover 23.5 g of the sample, which was collected by filtration using a 0.45 탆 filter and then concentrated using a rotary vacuum concentrator (Eyela A-1000S, Tokyo Rikakikai Co., Tokyo, Japan) And stored at -20 ° C.

<1-7> Manufacture of 30% Ethanol Extract of Cha Electronics

The impurities were removed and 32 g of well-dried tea was prepared. 4 to 8 times of 30% ethanol aqueous solution was added and the mixture was refluxed at 60 to 70 ° C for 3 to 5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less. Finally, 30 ml of distilled water was added to suspend the suspension, followed by freeze-drying to obtain a final brown powder.

Preparation Example 2 Preparation of hot water extract of KH-204

32 g of corn syrup, 32 g of ginger, 16 g of bokbunja, 16 g of alum and 4 g of omija were prepared, and 4 to 8 times of water was added thereto, and the mixture was refluxed at 100 to 120 ° C for 3 to 5 hours . The extract was filtered, and the filtrate was concentrated under reduced pressure at 50 to 60 ° C or less. The solution was boiled for 2 hours in a hot water bath (Daewoong, Korea) and centrifuged at 3,000 rpm. The supernatant was collected by filtration using a 0.45 ㎛ filter, and then washed with a rotary vacuum concentrator (Eyela A-1000S , Tokyo Rikakikai Co., Tokyo, Japan). After recovering the sample, 23.5 g of the sample was recovered by freeze-drying (ILSHIN, Korea) and stored at -20 ℃.

<Example 1> Cytotoxicity measurement in TM3 cells

MTT assay of mouse testicular cell TM3 confirmed the cytotoxicity of the sample. TM3 cells (KCLB21714) were sold in the Korean Cell Line Bank. Fetal bovine serum (FBS), Dulbecco's modified Eagle Medium (DMEM), 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide, MTT ). The ELISA microplate reader was a VersaMax (Molecular Device, USA).

More specifically, to assess the cytotoxicity of the sample using the testicular cell of TM3 cells of male mice Sladowski etc. (Sladowski D, et. Al. J Immunol Methods 157, 203-207. (1993)) by way of the MTT Analysis. The cells were treated with 200 μl of each of the samples at a concentration of 62.5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm, 2000 ppm, and 4000 ppm in 180 μl (2 × 10 ⁵ cells) of TM3 cells in a 96-well plate After culturing at 37 for 24 hours, 20 쨉 l of MTT solution (5 ㎎ / ml) was added and cultured at 37 캜 for 3 hours. After the supernatant was removed, 200 μl of DMSO was added to each well and reacted for 20 minutes. The survival rate was calculated by measuring the absorbance at 570 nm in an ELISA reader. The OD (optical density) value at this time is defined as the cell survival rate of 100%, and the measured OD value of the remaining group is converted into a relative value using the value of the normal group (the group treated with PBS instead of the sample treatment) As follows.

Cell survival rate (%) = (experimental value / control group) X 100

The cytotoxicity results of each sample are shown in the following Table 1 and FIG. 6 to FIG.

Control group 62.5 125 250 500 1000 2000 4000 (ppm) KH-204 Ethanol Extraction 100.00 0.09 89.40 ± 0.016 89.86 + 0.036 90.02 + 0.020 85.41 + 0.041 86.26 + 0.014 86.22 + 0.061 73.58 + 0.025 (%) KH-204
Hot water extraction 100.00 0.09 86.36 ± 0.009 87.07 + 0.041 86.74 + 0.039 87.79 + 0.031 88.60 + 0.011 87.55 ± 0.008 78.25 ±
0.060 (%) Corn oil 100.00 0.09 86.64 ± 0.051 85.75 ± 0.007 85.12 ± 0.003 85.20 + 0.014 84.16 + 0.025 68.62 + 0.019 61.71 ± 0.057 (%) Schisandra 100.00 0.09 96.47 ± 0.075 92.01 + - 0.144 88.49 + 0.095 83.94 + 0.080 82.39 + 0.195 79.49 + 0.019 78.70 ± 0.003 (%) Bokbunja 100.00 0.09 92.23 + 0.033 69.98 + 0.034 61.88 + 0.035 62.07 + 0.013 52.20 ± 0.081 47.98 + 0.105 35.32 + - 0.113 (%) Tosa 100.00 0.09 102.44 ± 0.006 99.13 + - 0.059 80.00 ± 0.001 65.58 ± 0.077 59.96 + 0.110 57.48 ± 0.004 52.83 + - 0.058 (%) Car electronics 100.00 0.09 99.29 + 0.073 90.84 + 0.010 81.16 + 0.087 75.35 + 0.048 71.32 + 0.018 69.10 + 0.065 67.87 ± 0.056 (%) Gugija 100.00 0.09 94.41 + 0.982 85.20 + - 0.874 85.16 + 0.892 82.03 + - 0.840 80.21 + - 0.822 79.22 + - 0.812 77.30 ± 0.793 (%)

The cell survival rate of TM3 cells was determined by comparing the cell viability of the control group with that of the untreated control group. The cytotoxicity was evaluated when the number of cells was decreased by 20% or more.

As a result, as shown in Table 1, the survival rate of the KH-204 ethanol extract sample was 73.58% at the concentration of 4000 ppm, and the survival rate of the KH-204 hot water extract sample at the concentration of 4000 ppm was 78.25% Respectively. The survival rates at 2000 ppm were 68.2%, 79.49% and 79.22%, respectively. Survival rates of tazza and chaeton were found to be 65.58% and 75.35% at 500 ppm, respectively. The survival rate of the bokbunja was found to be 69.98% at the concentration of 125 ppm.

It was determined that there is no toxicity in KH-204 ethanol, KH-204 hydrothermal solution, Sansui oil, Omija, and Gugija when the cell viability is below 80% It was confirmed to be toxic. However, the toxicity of KH-204, a mixture of them, was not confirmed, and the following in vivo experiments and in vivo experiments were carried out.

< Example  2> HUVEC in NO  Concentration measurement

In order for the penis to erect, blood must first be accumulated in the complete relaxation of the penis cavernosal artery and the smooth muscle of the penis and in the sinusoidal vascular space, and relaxation of the penis smooth muscle is accomplished by neurotransmitters and non-neurotransmitters. Recently, it has been found that the regulation of smooth muscle in the corpus cavernosum by prostaglandin and EDRF (endothelium derived relaxing factor) plays an important role in the erection. Palmer et al. Have shown that nitric oxide (NO) is one of the powerful EDRFs, and the role of NO in relaxation of the smooth muscle of corpus cavernosum has been actively studied. NO is biosynthesized by the biochemical action of NOS (nitric oxide synthase) in the body. Immunohistochemistry revealed that NOS - containing neurons and their organs were present and their distribution was similar in neuroanatomical studies in rats and humans. NO, a factor that promotes vascular smooth muscle relaxation in the corpus cavernosum tissue and improves erectile function, is an incomplete and unstable substance and can not be directly quantified because its half life is very short. Therefore, the content of nitrite, which is the metabolite in the body, By measuring, the content of NO can be expected. HUVEC cells were used to determine the effect of NO on the production of eNOS.

To measure NO concentration in HUVEC, total nitric oxide assay kit was used. HUVEC 900 (110 ⁴ cells) per well was added to each well of a 12-well plate and cultured in a 37, 5% CO ₂ incubator for 24 hours. Then, the medium was discarded and the surface of the cultured cells was washed with 1X PBS. Samples dissolved in 1X PBS were mixed with EGM-2 to a final concentration of 0, 50, 100, 200, 400 ppm and cultured in 37, 5% CO ₂ incubator for 48 hours. NADH 25 and nitrate reductase 25 were added to the culture medium 50 and incubated at 37 for 30 minutes. Griess reagent 50 and Griess reagent 50 were added thereto. The mixture was well mixed and allowed to stand at room temperature for 10 minutes. At the end of the reaction, absorbance was measured at 550 to calculate the concentration of nitrate, and nitrate was measured as an indicator of NO production.

The concentration of KH-204 ethanol and hot water extract used and the concentration of red ginseng in the control group are shown in Table 2 below.

group Experimental concentration KH -204 30% ethanol extract 0, 50, 100, 200, 400 ppm KH -204 hot water extraction 0, 50, 100, 200, 400 ppm Red ginseng (positive control group) 400 ppm

As a result, the results of NO production in the control red ginseng for each concentration of KH-204 ethanol and hot water extract are shown in Tables 3, 9 and 10 below.

0 (negative control group) 50 100 200 400 400 red ginseng
(Positive control group) (ppm) KH-204 Ethanol Extract 0.002 ± 0.092 3.877 + 0.783 6.038 + 0.468 7.508 + 0.065 16.369 ± 0.609 8.362 + 0.020 (%) KH-204
Hot water extract 0.002 ± 0.092 0.031 0.109 0.454 + 0.403 2.438 ± 0.555 7.438 + - 0.533 8.362 + 0.020 (%)

As a result, the concentration of nitrate was found to be about 3.877, 6.038, 7.508, and 16.369 μmol / ㎖ as the samples of KH-204 30% ethanol extract were treated at 50, 100, 200 and 400 ppm. The negative control group had a result of 0.002 μmol / ml, and the KH-204 30% ethanol extract group showed an increase in the nitrate concentration (FIG. 9).

The concentration of nitrate was about 0.031, 0.454, 2.438, and 7.438 μmol / ㎖, respectively, when treated with 50, 100, 200 and 400 ppm of the hot water extract of KH-204 and compared with the negative control (Fig. 10), as did KH-204 30% ethanol extracts.

In particular, the concentration of KH-204 30% ethanol extract at 400 ppm was 16.369 μmol / ㎖, which was higher than that of 400 ppm of red ginseng used as a positive control, 8.362 μmol / ㎖.

The KH-204 hydrothermal extracts showed higher nitrate concentration than the negative control group but lower than the nitrate concentration of the positive control group of 400 ppm red ginseng. It was found that nitrate was produced more effectively when compared. Test results suggest that the samples improve the function of the penis by increasing the NO production, a vasodilator.

< Example  3> ACE  ( Angiotensin converting enzyme ) Inhibition effect measurement

In order to maintain the relaxation state of the vascular muscles, which is the core of the erection maintenance, which is deeply related to male sexual function improvement and strengthening, it is effective to inhibit the action of ACE involved in the synthesis of angiotensin, a strong vasoconstrictor, To date, some ACE inhibitors have been proposed as candidate substances for sexual enhancement and enhancement. Angiotensin-converting enzyme (ACE; peptodyldipeptide hydrolase EC 3.4.15.1) is an enzyme that plays a crucial role in regulating blood pressure. This enzyme increases blood pressure by hydrolyzing angiotensin, which consists of 10 amino acids, into angiotensin. The strong angiotensin, angiotensin, promotes the secretion of aldosterone. As a result, aldosterone increases the retention of Na ⁺ and water in the kidneys and leads to an increase in arterial blood pressure. If you inhibit angiotensin production by inhibiting ACE, you may lower your blood pressure. In addition, relaxation of the blood vessels leads to increased blood flow in the penis, which leads to erection of the penis. In this test, N-Hippuryl-His-Leu was used instead of ACE substrate Angiotensin and ACE was extracted from lung tissue powder of rabbit and used as enzyme solution. The inhibition rate was confirmed by measuring the hippuric acid produced from the enzyme reaction at 228 nm (I., M., Y., Shon and S., H., Na., J Korean Soc Food Sci Nutr . 36, 1511-1516. (2007); E., K., Cho et. al, J. Life Sci . 21, 811-818. (2011); C. Liang et. al., Natural product sciences , 17 (4), 363-366. (2011).

The principle is summarized as follows.

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu ( Angiotensin ) + ACE ( Enzyme )

↓

Asp-Arg-Val-Tyr-He-His-Pro-Phe ( Angiotensin ) + His-Leu: product

Hippuric acid-His-Leu (N-Hippuryl-His-Leu) + ACE ( Enzyme )

↓

Hippuric acid + His-Leu: product

Sigma and hippuryl-histidyl-leucine (HHL) were purchased from Sigma-Aldrich and boric acid, rabbit lung acetone powder, and ultrapure water from PURELAB Plus were used. Other reagents used were Extra pure grade reagents. The UV-VIS spectrophotometer was Spectronic Genesys 5 (Milton Roy Co., Ivyland, USA).

The samples, extraction conditions and concentrations used in this test are summarized in Table 4 below.

sample Extraction condition Manufacturing concentration ppm Final concentration ppm KH-204 30% ethanol 2500 500 KH-204 Heat number 2500 500 Schisandra 30% ethanol 2500 500 Gugija 30% ethanol 2500 500 Car electronics 30% ethanol 2500 500 Bokbunja 30% ethanol 2500 500 Tosa 30% ethanol 2500 500 Corn oil 30% ethanol 2500 500 Enalapril maleate salt
(Positive control group) 2500 25

Samples were dissolved in distilled water to 2500 ppm and stored at 4 ° C until use. This experiment was conducted by Cho et al. (E., K., Cho et al., J. Life Sci . 21, 811-818. (2011)). Rabbit lung acetone powder (Sigma) was extracted with 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl at a concentration of 0.1 g / ml (w / v) and then extracted at 4 ° C for 2 hours. After centrifugation at 4 ° C for 40 minutes at 4000 rpm, the supernatant was used as the ACE enzyme solution. The substrate was prepared by dissolving hippuryl-histidyl-leucine (HHL) at a concentration of 5 mg / ml (w / v) in 0.1 M sodium borate buffer (pH 8.3) containing 0.3 M NaCl. ACE inhibitory activity was determined by adding 50 μl of ACE enzyme solution to the sample, preliminarily reacting at 37 ° C for 5 minutes, adding the substrate solution, and reacting at 37 ° C for 30 minutes. 150 μl of 0.1 M HCl was added to stop the reaction, 750 μl of ethyl acetate was added, and the mixture was stirred for 15 seconds and centrifuged at 4,000 rpm for 5 minutes. The supernatant was completely dried and dissolved in 3 ml of distilled water and absorbance was measured at 228 nm. As a negative control, distilled water was used instead of crude enzyme, and Enalapril maleate salt was used as a positive control. The inhibition rate of ACE was calculated using the following formula.

ACE inhibition rate (%) = [1- (ACE test / ACE control)] X 100

As a result, the ACE inhibition rate of each sample was described in 5 and 11 below.

Sample name Extraction condition density ACE inhibition rate (%) KH-204 Heat number 500 ppm 47.0 ± 8.2 KH-204 30% ethanol 34.2 ± 2.7 Schisandra 30% ethanol 45.9 ± 6.8 Gugija 30% ethanol 49.1 ± 8.2 Car electronics 30% ethanol 36.3 ± 8.2 Bokbunja 30% ethanol 29.9 ± 2.7 Tosa 30% ethanol 12.8 ± 0.0 Corn oil 30% ethanol 16.0 ± 1.4 Enalapril maleate salt (positive control) 25 ppm 98.3 ± 5.4

The inhibition rate was 5 mg / ml of hippuryl-histidyl-leucine (HHL) as a substrate, and 0.1 g / ml of ACE enzyme solution was reacted at 37 ° C for 30 minutes and absorbance was measured at 228 nm. (49.1%), KH-204 hot water extraction (47.0%), omija (45.9%), tea extract (36.3%), KH-204 30% ethanol extraction (34.2%) and bokbunja 29.9%). Therefore, it is thought that six samples are helpful to maintain the erection by increasing the blood flow of the penis indirectly through the relaxation of the blood vessel.

< Example  4> PDE  ( Phosphodiesterase ) -5 Inhibition rate measurement

Penile erection is caused by the complex interaction of physiological processes involving the central nervous system, peripheral nervous system and smooth muscle. In particular, the release of nitric oxide (NO) from nonadrenergic, noncholinergic and endothelial cells activates guanylate cyclase in the corpus cavernosum and increases intracellular cGMP (cyclic GMP) levels. Increased intracellular cGMP reduces intracellular calcium levels, which leads to luminal smooth muscle relaxation, which in turn leads to penile erection through spongy body volume expansion. Since cGMP is degraded by PDE (phosphodiesterase) -5, its activity should be suppressed to increase the concentration of cGMP and improve erectile function. In this test, 5-GMP is produced by PDE-5 using 3,5-cGMP as a substrate, which is phosphorylated by 5-nucleotidase. The amount of phosphoric acid produced was measured to determine how much the inhibition of PDE-5 activity was inhibited. Based on the results, the data were used to improve male infertility (FIG. 12).

The UV-VIS spectrophotometer used for the absorbance measurement was a Spectronic Genesys 5 (Milton Roy Co., Ivyland, USA) model, and the ELISA microplate reader VersaMax (Molecular Device, Sunnyvale, USA) was used.

The medicinal samples containing KH-204 ethanol and hot-water extract used in this test, extraction conditions and concentrations are summarized in Table 6 below.

sample Extraction condition Concentration (ppm) Final concentration (ppm) KH-204 30% ethanol 2500 500 KH-204 Heat number 2500 500 Gugija 30% ethanol 2500 500 Bokbunja 30% ethanol 2500 500 Corn oil 30% ethanol 2500 500 Schisandra 30% ethanol 2500 500 Tosa 30% ethanol 2500 500 Car electronics 30% ethanol 2500 500 IBMX (positive control) 9

Samples are stored at 4 ℃ was dissolved in distilled water until the make 2500 ppm, PDE inhibitory activity in accordance with the method such as Jang (. JD Corbin and SH Francis . J. Andrology. 24, S38-S41 (2003)) cyclic nucleotide phosphodiesterase assay kit. 5-GMP was treated with 5-nucleotidase followed by treatment with BIOMOL GREEN reagent and absorbance was measured at 620 nm to obtain a standard curve. After adding 3,5-cGMP to the PDE and each sample, -nucleotidase, treated with BIOMOL GREEN reagent, absorbance was measured at 620 nm, and the amount of 5-GMP produced was compared with the control group to calculate the PDE inhibition rate. The negative control group was excluded from the substrate and the positive control group was 3-isobutyl-1-methylxanthine (IBMX) as a positive control. The IC ₅₀ value of IBMX was 5.5 ppm.

PDE-5 inhibition rate (%) = [1- (PDE test - sample blank / control - control blank)] X 100

As a result, the obtained standard curve is shown in Table 7 and FIG.

5-GMP (n) O.D. 620 3.00 0.6640 2.00 0.5045 1.50 0.3844 1.00 0.2647 0.75 0.2427 0.50 0.1583 0.25 0.1121 0 0.0708

As a result, the obtained PDE inhibition rate measurement results are shown in Table 8 and FIG.

Sample name Extraction condition density  Inhibition rate (%) KH-204 Heat number 500 ppm  -3.0 ± 1.2 KH-204 30% ethanol  25.6 ± 1.4 Tosa 30% ethanol 13.5 ± 1.6 Gugija 30% ethanol 32.8 ± 0.5 Car electronics 30% ethanol -3.40 ± 1.3 Bokbunja 30% ethanol 34.5 ± 2.92 Schisandra 30% ethanol 35.6 ± 1.6 Corn oil 30% ethanol -15.3 ± 1.8 IBMX (positive control) 9 ppm 94.7 ± 3.6

As a result, the IC _{50 of} Sildenafil, now known as the erectile dysfunction substance, The value is 3.5 nM and the IC ₅₀ of Vardenafil The value is 6.6 nM and the IC ₅₀ of Tadalafil The value was reported as 0.2 nM. The PDE-5 inhibitory effect was better in the order of bokbunja (34.5%), omija (35.6%), gojia (32.8%) and KH-204 (30% ethanol extraction, 25.6%).

Thus, the in vitro test was completed, and the in vivo test was performed in the following animal test.

< Example  5> Animal experiments (long-term weighing and sperm function measurement)

The effects of KH-204 on general animal models were verified. The concentrations of the KH-204 ethanol extract and the control red ginseng used were as shown in Table 9 below.

group Negative control group Sample treatment group Positive control group  density 0 (/) 50 (/) 100 (/) 200 (/) 400 (/) Red ginseng 400 (/)

ICR mice (male, 8 weeks old) were purchased from Orient Bio Co., Ltd. (Gapyeong, Gyeonggi-do) and adapted for 1 week and used in the experiment. During the experimental period, water and feed were freely taken, the temperature of the feeding room was 22 ± 2 ℃, the humidity was 55 ± 5% and the intensity of light was automatically adjusted to 12 hours.

Specifically, the samples were orally administered once daily for 8 days to each of the negative control group to which sterilized water was administered, the sample treatment group to which KH-204 (30% ethanol extract) was administered, and the group to which red ginseng was administered as a positive control group. Male mice were divided into two groups: 50, 100, 200 and 400 ㎎ / ㎏. Six mice were used per group. Once a day for 8 days. After 24 hours of the last oral administration, the mice were weighed and sacrificed. The left testis, left atrium, and left seminal vesicle were weighed and their weights were measured. The isolated atypse was homogenized in 10 ml of DMEM medium, Ml was dispensed into 24 wells, and 10 minutes later, the number of spermatids was measured on a 200 magnification microscope.

<5-1> Mouse Weight

As a result, the final weight of the mouse is shown in Table 10 and FIG.

Control group 50 (mg / kg) 100 (mg / kg) 200 (mg / kg) 400 (mg / kg) Red ginseng 400 (mg / kg) Weight (g) 37.01 + 1.63 36.53 ± 1.09 36.73 ± 1.13 34.61 + - 1.85 29.29 + 1.58 36.09 ± 1.08

As a result, when the weight of the mouse was measured at the last day, it was 34.61 g and 29.29 g when 200 (mg / kg) and 400 (mg / kg) KH-204 (ethanol extract) , Especially in the group administered 400 (mg / kg), showed a weight reduction of about 7 g, which was about 20%.

<5-2> Organs (seminal vesicle) weight

As a result, the weight of the seminal vesicles of the mice is shown in Table 11 and FIG. 16 below.

Control group 50 (mg / kg) 100 (mg / kg) 200 (mg / kg) 400 (mg / kg) Red ginseng 400 (mg / kg) The seminal vesicle (g) 0.075 0.02 0.078 ± 0.01 0.084 0.02 0.089 0.02 0.091 ± 0.01 0.094 + - 0.01

As a result, the seminal vesicle weight of mice tended to increase as the concentration of KH-204 (ethanol extract) was higher.

<5-3> Organs (testes) Weight

As a result, the testimony weight of the mice is shown in Table 12 and FIG.

Control group  50 (mg / kg) 100 (mg / kg) 200 (mg / kg) 400 (mg / kg) Red ginseng 400 (mg / kg) Testis (g) 0.11 + - 0.01 0.11 + 0.02 0.11 + - 0.01 0.13 + - 0.01 0.12 + - 0.01 0.13 ± 0.00

As a result, mouse testis weighed the highest in the group administered with 200 (mg / kg) KH-204 (ethanol extract) and the same results as the group administered with 400 (mg / kg) red ginseng.

<5-4> Long term (negative) weight

The results are shown in Table 13 and Fig. 18 below.

Control group  (Mg / kg) 0 (mg / kg) 0 (mg / kg) 0 (mg / kg) 400 mg (mg / kg) No (g) 0.040 0.000 0.041 ± 0.002 0.042 ± 0.004 0.041 ± 0.002 0.037 ± 0.002 0.042 ± 0.004

As a result, there was almost no difference among the groups of mouse nocturnal lesions, but in the group administered with 400 (mg / kg) KH-204 (ethanol extract)

<5-5> Number of sperm

As a result, the number of spermatozoa of the mice is shown in Table 14 and FIG.

Control group  (Mg / kg) 0 (mg / kg) 0 (mg / kg) 0 (mg / kg) 400 mg (mg / kg) Number of sperm (horses) 94.80 + - 7.19 106.33 + - 8.59 118.60 ± 18.66 123.00 ± 14.52 127.00 ± 16.31 103.80 ± 10.43

As a result, the number of spermatids of the mice tended to increase with increasing concentration of KH-204 (ethanol extract), and that of KH-204 (ethanol extract) A large number of sperm counts were observed in the group.

<5-6> Sperm movement

As a result, sperm motility of the mouse is shown in Table 15 and FIG. 20 to FIG.

Control group  (Mg / kg) 0 (mg / kg) 0 (mg / kg) 0 (mg / kg) 400 mg (mg / kg) Sperm motility (%) 53.73 ± 16.69 68.46 + - 3.52 70.88 ± 6.12 74.09 + - 8.20 91.12 + - 2.56 66.50 + - 8.56

As a result, sperm motility of the mice tended to increase as the concentration of KH-204 (ethanol extract) increased, and that of KH-204 (ethanol extract) was higher than that of the red ginseng 400 (mg / High sperm motility was confirmed in the group.

<Other Manufacturing example  1> KH -204 Preparation of male infertility treatment agent containing ethanol extract as an active ingredient

The inventors of the present invention confirmed that the extract of KH-204 ethanol was excellent in the therapeutic effect of male infertility through the above examples, and thus a male infertility treatment agent containing KH-204 ethanol extract as an active ingredient was prepared as follows. In addition, the preparation examples of the following therapeutic agents can be applied not only to therapeutic agents but also to the production of health foods.

<1-1> KH -204 soft capsule containing the ethanol extract ( soft gelatin capsules )

KH-204 ethanol extract 20%, vitamin C 4.5%, vitamin D ₃ 0.001%, manganese sulfate 0.1%, wax 15%, palm oil 30%, safflower seed oil 30.399%

 <1-2> KH Preparation of an intravenous formulation containing an ethanol extract of -204

2% of KH-204 ethanol extract, 3% of mannitol, 95% of physiological saline,

<1-3> KH -204 Tablets containing ethanol extract ( tablet )

KH-204 ethanol extract 35%, vitamin C 15%, vitamin D ₃ 0.001%, manganese sulfate 0.1%, crystalline cellulose 25.0%, lactose 21.999%, magnesium stearate 2.9%

<Other Manufacturing example  2> KH -204 Preparation of Functional Foods Containing Ethanol Extract as Active Ingredients

The inventors of the present invention confirmed that the KH-204 ethanol extract was superior to the male infertility treatment through the above examples, and prepared a functional food containing the active ingredient as follows.

<2-1> Production of beverage

Inositol 30 mg, Orthoic acid 50 mg, KH-204 ethanol extract 1.2 mg, water 200 mL, the composition of the above composition (1), the above composition &Lt; / RTI &gt; and the ingredients were prepared using conventional methods.

<2-2> Of chewing gum  Produce

Chewing gum was prepared using a conventional method with the above composition and content as follows: gum base 40%, sugar 56.36%, KH-204 ethanol extract 0.64%, flavor 1%, water 2%

<2-3> Manufacture of candy

60% of sugar, 39.26% of starch syrup, 0.64% of KH-204 ethanol extract, and 0.1% of orange flavor. The above ingredients and contents were used to prepare candies using a conventional method.

<2-4> Biscuit  Produce

The breads were divided into two groups: first class 88 ㎏, gravity first class 76.4 ㎏, white sugar 16.5 ㎏, salted 2.5 ㎏, glucose 2.7 ㎏, palm shortening 40.5 ㎏, ammonia 5.3 ㎏, medium diet 0.6 ㎏, sodium bisulfite 0.55 ㎏, rice flour 5.0 ㎏, , 0.23 kg of vitamin B2, 0.16 kg of milk fractions, 71.1 kg of water, 4 kg of whole milk powder, 1 kg of substitute milk powder, 0.1 kg of calcium phosphate, 1 kg of spray salt, 25 kg of sprayed milk, 0.5 kg of KH-204 ethanol extract The biscuits were prepared using the conventional method in terms of composition and content.

<2-5> Production of ice cream

(100%), milk fat solid 10.4%, sugar 12.0%, starch syrup 3.0%, span, span 0.5%, flavor (Strawberry) 0.15%, water 63.31%, KH-204 ethanol extract 0.64% &Lt; / RTI &gt; and the content thereof.

<2-6> Of chocolate  Produce

Chocolate, 34.36%, 34% cocoa butter, 15% cocoa mass, 15% cocoa powder, 0.5% lecithin, 0.5% vanilla flavor and 0.64% KH-204 ethanol extract .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, This is possible.

Claims (7)

A pharmaceutical composition for improving male sexual function and treating infertility, which comprises at least one extract selected from the group consisting of corn oil extract, Gugija extract, bokbunja extract, alum extract, Omiza extract and tea extract.
2. The pharmaceutical composition according to claim 1, wherein the extract further comprises a pharmaceutically acceptable carrier.
[Claim 2] The pharmaceutical composition according to claim 1, wherein the composition comprises an extract of corn oil, a coriander extract, a bokbunja extract, a coriander extract, and an omija extract.
[Claim 4] The composition according to claim 3, wherein the composition comprises 50 to 150 parts by weight of gum arabic, 20 to 100 parts by weight of brambles, 20 to 100 parts by weight of humus, and 10 to 50 parts by weight of omiza, based on 100 parts by weight of the extract. .
The pharmaceutical composition according to claim 1, wherein the composition comprises a tea extract as an active ingredient.
6. The pharmaceutical composition according to any one of claims 1 to 5, wherein the extract is water or an ethanol extract.
A composition for improving male sexual function and preventing infertility comprising, as an active ingredient, one or more extracts selected from the group consisting of food-borne nutrient-containing additives, gugija extract, bokbunja extract, tosaja extract, omija extract and tea extract .

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