KR20140089242A - Culture method of endoclyta exerescens cordyceps sinensis and endoclyta exerescens cordyceps sinensis by the culture method - Google Patents

Abstract

The present invention relates to a culture method of a mycelium of Cordyceps sinensis parasitic on a larva of Endoclyta excrescens, and the mycelium of Cordyceps sinensis parasitic on the larva of Endoclyta excrescens cultured thereby. More specifically, the culture method of the mycelium of Cordyceps sinensis parasitic on the larva of Endoclyta excrescens comprises: a washing step for washing Cordyceps sinensis parasitic on the larva of Endoclyta excrescens; a Cordyceps sinensis fluid preparation step for grinding the Cordyceps sinensis obtained through the washing step, and mixing distilled water thereto to prepare Cordyceps sinensis fluid; a culture medium preparation step for heating a mixture which contains grain flour, protein, glucose, potassium phosphate, magnesium sulfate, and agar to dissolve the mixture, and pouring the mixture which has been dissolved on the surface of glass to prepare a culture medium, followed by sterilizing the culture medium; and a culture step for inoculating the culture medium prepared in the culture medium preparation step with the Cordyceps sinensis fluid prepared in the Cordyceps sinensis fluid preparation step, and culturing the mycelium of the Cordyceps sinensis.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for culturing mycelium of Bat moth, Mycobacterium tuberosum, and mycelia of Bat moth, Mycobacterium avigrebifolia,

The present invention relates to a method for culturing mycelium of Bat moth, Mycobacterium avigilis, and a culture method thereof. More particularly, the present invention relates to a method for culturing Mycobacterium tuberosus Mycelia And mycelium of bacterium Cordyceps mucilothi cultured by the culture method.

The present invention relates to a method for culturing Mycelium of Bat moth, Mycobacterium tuberculosis, and a culture method thereof. More particularly, the present invention relates to a method for culturing mycelium of Bat moth, Mycobacterium tuberosus, And mycelium of bacterium Cordyceps mucilothi cultured by the culture method.

Worldwide, there are more than 300 species of caterpillar fungus, which are used for net medicines and beverages except 9, 10, and 10 kinds of fungus have.

In the conventional methods described above, Cordyceps was inoculated into various larvae or pupae to obtain a left insect, but it has not been successful in mass production in repetitive and stable state.

In addition, some experiments have succeeded in producing lefty bodies by inoculating or infecting dragon caterpillars with larvae or pupae, but the method of mass production repeatedly has not succeeded yet.

However, if the mycelium grows to a certain height by inoculating the spores and mycelia with rice or silkworm in and out of the siltworm pupa, the products processed with the left and pupa are sold in the market as medicines or foods However, this is significantly lower than that of common Cordyceps species as a dragon cunechus, and it is not recognized as a medicinal caterpillar fungus in the academic scene.

Cordyceps has been known as the raw material of precious Jangseongbulro from China's Ming Dynasty to the present day. In the past, it was used only as medicinal material, food and cosmetic material in the royal palace, but the production amount was not enough to be consumed due to lack of raw material resources .

It is an object of the present invention to provide a method for culturing Mycelium of Bat moth, which shows the content and efficacy of an effective ingredient similar to that of natural Cordyceps, and to a mycelium of Bat moth, cultured by the method.

Another object of the present invention is to provide a method for culturing Mycelium of Bat moth, which is mass-producible and low in manufacturing cost, and a Mycelium of Bat moth, cultured by the method.

The object of the present invention is to provide a method for preparing a Chinese cabbage, comprising the steps of washing a moth caterpillar, a step of preparing a Chinese cabbage to prepare a Chinese cabbage stock after mixing with distilled water after polishing the cabbage caterpillar, , Magnesium sulfate and agar is heated and dissolved and then the dissolved mixture is poured into a glass surface to prepare an incubator and a sterilization treatment is carried out and a caterpillar fungus prepared in the step of preparing the caterpillar fish preparation is prepared And culturing the cultured Mycelium of moth, Mycobacterium tuberculosis, Mycobacterium tuberculosis, and the like.

According to a preferred feature of the present invention, the washing step is performed by washing the bat moth corymbroside with purified water, followed by washing with alcohol cotton.

According to a more preferred feature of the present invention, in the step of preparing the Chinese cabbage, the bat moth cordis, which has undergone the washing step, is newly cut, the section of the cut cordyceps is dug out, And 100 to 150 parts by weight of distilled water is added to the weight portion and then further polished.

According to a further preferred feature of the present invention, in the step of preparing the culture medium, the content of the cereal flour is 3 to 5 parts by weight based on 100 parts by weight of the whole incubator, 0.3 to 0.5 parts by weight of protein copper, 1 to 3 parts by weight of glucose, To 0.2 part by weight, magnesium sulfate in an amount of 0.01 to 0.5 part by weight and agar in an amount of 1 to 3 parts by weight is heated and dissolved and then poured on a glass surface to prepare an incubator and sterilized.

According to an even more preferred feature of the present invention, the grain flour is made of one selected from the group consisting of bran, sulfur, and potato starch.

According to a further preferred feature of the present invention, the sterilization treatment is performed by irradiating the incubator with ultraviolet rays for 30 minutes at a temperature of 30 ° C from the incubator at a room temperature, after autoclaving the incubator for 30 minutes at a temperature of 120 ° C .

According to still another more preferred characteristic of the present invention, in the culturing step, the Cordyceps sinensis prepared in the step of preparing Cordyceps sinensis is inoculated in an incubator manufactured in the step of producing the culture medium at a concentration of 8 to 12.5% To 160 times, and is cultured at a temperature of 23 to 28 DEG C for 5 to 7 days.

In addition, the object of the present invention can also be achieved by providing a mycelia of Bat moth caterpillar fungus, which is cultured by the method of culturing the Bat moth caterpillar fungus

The method of culturing mycelia of Bat moth caterpillar fungus according to the present invention and the mycelia of Bat moth caterpillar cultured by the method of the present invention shows an excellent effect of providing the Bat moth caterpillar fungus body showing the content and effect of the active ingredient similar to natural caterpillar fungus.

The present invention also provides an excellent effect of providing a method for culturing mycelia of Bat moth caterpillar fungus, which can be mass-produced, at low manufacturing cost, and mycelia of Bat moth caterpillar cultured by the culturing method.

FIG. 1 is a flowchart showing a method for culturing mycelium of bat moth caterpillar fungus according to the present invention.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

The method for culturing the mycelia of Bat moth caterpillar fungus according to the present invention comprises a washing step (S101) for washing Bat moth caterpillar fungus, a method for cultivating Cordyceps sinensis by mixing the distilled water after polishing the Bat moth caterpillar fungus through the washing step (S101) (S103), an incubator preparation step (S105) of heating and dissolving a mixture containing cereal flour, protein copper, glucose, potassium phosphate, magnesium sulfate and agar, and then pouring the dissolved mixture on a glass surface to prepare an incubator and disinfecting the mixture And a cultivation step (S107) of inoculating and cultivating the Chinese cabbage stock prepared in the step (S103) of cultivating the Chinese cabbage extract into the incubator manufactured in the culture stage (S105).

The washing step (S101) is a step of washing the Bat moth caterpillar fungus. The Bat moth caterpillar fungus is washed with purified water and then washed with alcohol cotton. The foreign matter remaining on the surface of Bat moth Cordyceps is washed with sterilized purified water When the surface of Bat moth caterpillar is dried, it is a step of washing the surface of Bat moth caterpillar fungus using the alcohol cotton coated with alcohol with alcohol concentration of 75%.

The step S103 of producing the Chinese cabbage beet is a step of preparing the Chinese cabbage beet by mixing the distilled water after polishing the Chinese cabbage caterpillar fungus through the washing step S101, Cutting the cut cordyceps, cutting the cut cordyceps, crossing the cuts with sea sand, polishing with a pestle, and adding 100 to 150 parts by weight of distilled water to 100 parts by weight of the ground mixture.

At this time, it is preferable to cut the cordyceps using the non-surgical surgical knife which has been sterilized, and the cordyceps juice obtained through the above process is put into a 4 L capacity vial by using a pipette, Lt; 0 > C.

The incubator manufacturing step (S105) is a step of heating and dissolving a mixture containing grain powder, protein copper, glucose, potassium phosphate, magnesium sulfate and agar, and then dissolving the dissolved mixture on a glass surface to prepare an incubator, Wherein the content of the cereal flour is 3 to 5 parts by weight based on 100 parts by weight of the whole incubator, 0.3 to 0.5 parts by weight of protein copper, 1 to 3 parts by weight of glucose, 0.05 to 0.2 parts by weight of potassium phosphate, 0.01 to 0.5 parts by weight of magnesium sulfate, 1 to 3 parts by weight is heated to completely dissolve the mixture, and then poured onto a glass surface to prepare an incubator and sterilization treatment.

The cereal flour preferably comprises one selected from the group consisting of bran, sulfur, and potato starch. In the sterilization treatment, the incubator formed on the glass surface is autoclaved at a temperature of 120 ° C for 30 minutes, It is made by irradiating ultraviolet rays to the incubator for 30 minutes at a distance of 30 centimeters.

When the cereal flour is wheat bran, the cultivation completion period is shortened when the bran content is 4 to 5% in the incubator. When the grain flour is 4 to 6% in the case of the sulfur flour and between 3 and 6% %, The incubation completion period is shortened.

At this time, the glass surface may exhibit a shape of a tube or a flat shape and may be manufactured by a slope incubator or a flat incubator.

The culturing step (S107) is a step of inoculating and cultivating the Chinese cabbage stock prepared in the preparation step (S103) of the Chinese cabbage extract preparation step (S105) and the culture step (S103) And then incubating the culture for 10 to 20 days at a temperature of 23 to 28 캜 for 5 to 7 days after the seedling of the Chinese cabbage is inoculated into the incubator manufactured in the incubator manufacturing step (S105).

More specifically, the culturing step is carried out by inoculating a Chinese cabbage stock to an incubator manufactured in the form of a slope incubator or a flat incubator at a concentration of 8 to 12.5%, introducing the incubator into which the copper bonito freshly inoculated, 120 to 160 times per minute and is cultured at a temperature of 23 to 28 DEG C for 5 to 7 days.

When the culture medium contains wheat bran or yellowing powder, the inoculum concentration is preferably 10 to 12.5%, and in the case of potato starch, the incubation time is shortened when the inoculum concentration is 8.3 to 12.5%.

The mycelia of Bat moth caterpillar fungus cultured through the above culturing step (S107) are abundantly contained in Mandit, peptides, sugars, glucoside compounds, spoloids and amino acids.

Hereinafter, the method of culturing mycelia of Bat moth caterpillar fungus according to the present invention and the content of active ingredients will be described with reference to examples.

≪ Example 1 >

The bat moth caterpillar fungus (purchased from the agricultural trade market of Shenyang city) was washed with sterilized purified water, then washed with alcohol-coated alcohol 75%, and the washed caterpillar fungus was sterilized using a sterilized surgical knife , The cut surface of the Cordyceps is cut off and added with sea sand (0.1 to 1 part by weight based on 100 parts by weight of Cordyceps) to the root, and the root is ground with a pestle. 120 parts by weight of purified water is added to 100 parts by weight of the ground mixture The polished mixture was again poured into a 4L reagent bottle and stored at 4 ° C to prepare a freshwater pupa.

When the mixture of bran content of 4% and a mixture of protein copper, glucose, potassium phosphate, magnesium sulfate and agar was heated and completely dissolved, the slurry was poured into a slope glass surface and incubated at 120 ° C for 30 minutes , The incubator was autoclaved at room temperature under a sterilization condition by irradiating ultraviolet rays for 30 minutes at a distance of 30 cm from the slope incubator for 30 minutes.

The above-mentioned Cordyceps specimens were inoculated into the incubator at a concentration of 11%, and cultured for 14 days at 24 ° C for 8 days.

≪ Example 2 >

The procedure of Example 1 was followed except that the bran content was adjusted to 5%.

≪ Example 3 >

The procedure of Example 1 was followed except that the culture was carried out using sulfur instead of wheat bran.

<Example 4>

Proceeding in the same manner as in Example 3, the culture was carried out at a content of 5% of sulfur.

&Lt; Example 5 >

Proceeding in the same manner as in Example 3, the culture was carried out at a content of 6% of sulfur.

&Lt; Example 6 >

The procedure of Example 1 was followed except that potato starch was used instead of wheat bran, and the content of potato starch was adjusted to 3%.

&Lt; Example 7 >

Proceeding in the same manner as in Example 6, culturing was continued at 4% of the potato starch content.

&Lt; Example 8 >

The procedure of Example 6 was followed except that the content of potato starch was adjusted to 5%.

&Lt; Example 9 >

Proceeding in the same manner as in Example 6, the cultivation was continued at a content of potato starch of 6%.

&Lt; Comparative Example 1 &

The procedure of Example 1 was followed except that the bran content was 2%.

&Lt; Comparative Example 2 &

Proceeding in the same manner as in Example 1, the culture was performed at a bran content of 3%.

&Lt; Comparative Example 3 &

The procedure of Example 1 was followed except that the bran content was set at 6%.

&Lt; Comparative Example 4 &

Proceeding in the same manner as in Example 3, the culture was carried out at a concentration of 1% of sulfur.

&Lt; Comparative Example 5 &

Proceeding in the same manner as in Example 3, culturing was carried out at a content of 2% of sulfur.

&Lt; Comparative Example 6 >

Proceeding in the same manner as in Example 3, the culture was continued at a content of 3% of sulfur.

&Lt; Comparative Example 7 &

Proceeding in the same manner as in Example 6, the culture was carried out with the content of potato starch set at 1%.

&Lt; Comparative Example 8 >

Proceeding in the same manner as in Example 6, the cultivation was continued at a content of 2% of potato starch.

Table 1 below shows the results of culturing conducted through Examples 1 to 9 and Comparative Examples 1 to 8.

<Table 1>

As shown in Table 1 above, it can be seen that the culture completion (++++) proceeds more rapidly in the culture conditions proceeding through Examples 1 to 9 of the present invention.

&Lt; Example 10 >

The procedure was carried out in the same manner as in Example 2, except that the culture was carried out at a temperature of 23 ° C.

&Lt; Example 11 >

The procedure of Example 2 was followed except that the incubation was carried out at a temperature of 26 ° C.

&Lt; Example 12 >

The procedure of Example 2 was followed except that the culture was carried out at a temperature of 28 ° C.

&Lt; Example 13 >

The procedure was carried out in the same manner as in Example 3 except that the culture was carried out at a temperature of 23 ° C.

&Lt; Example 14 >

The procedure was carried out in the same manner as in Example 3, except that the culture was carried out at a temperature of 26 ° C.

&Lt; Example 15 >

The procedure was carried out in the same manner as in Example 3 except that the culture was carried out at a temperature of 28 ° C.

&Lt; Example 16 >

The procedure of Example 8 was followed except that the culture was carried out at a temperature of 23 ° C.

&Lt; Example 17 >

The procedure of Example 8 was followed except that the culture was carried out at a temperature of 26 ° C.

&Lt; Example 18 >

The procedure of Example 8 was followed except that the culture was carried out at a temperature of 28 ° C.

&Lt; Comparative Example 9 &

The procedure of Example 2 was followed except that the culture was carried out at a temperature of 18 캜.

&Lt; Comparative Example 10 &

The procedure of Example 2 was followed except that the incubation was carried out at a temperature of 20 ° C.

&Lt; Comparative Example 11 &

The procedure of Example 2 was followed except that the incubation was carried out at a temperature of 30 ° C.

&Lt; Comparative Example 12 >

The procedure of Example 2 was followed except that the culturing was carried out at a culturing temperature of 32 ° C.

&Lt; Comparative Example 13 &

The procedure was carried out in the same manner as in Example 3, except that the culture was carried out at a temperature of 18 ° C.

&Lt; Comparative Example 14 >

The procedure was carried out in the same manner as in Example 3, except that the culture was carried out at a temperature of 20 ° C.

&Lt; Comparative Example 15 &

The procedure was carried out in the same manner as in Example 3 except that the culture was carried out at a temperature of 30 ° C.

&Lt; Comparative Example 15 &

The procedure was carried out in the same manner as in Example 3 except that the culture was carried out at a temperature of 30 ° C.

&Lt; Comparative Example 16 >

The procedure of Example 8 was followed except that the culture was carried out at a temperature of 18 캜.

&Lt; Comparative Example 17 >

The procedure of Example 8 was followed except that the culture was carried out at a temperature of 20 ° C.

&Lt; Comparative Example 18 >

The procedure of Example 8 was followed except that the incubation was carried out at a temperature of 30 ° C.

Table 2 shows the results of the cultivation conducted through Examples 10 to 18 and Comparative Examples 9 to 18.

<Table 2>

As shown in Table 2 above, it can be seen that the culture completion (++++) proceeds more rapidly in the culture conditions proceeding through Examples 10 to 18 of the present invention.

&Lt; Example 19 >

Proceeding in the same manner as in Example 2, culturing was carried out with an order of 120 for one minute.

&Lt; Example 20 >

Proceeding in the same manner as in Example 2, incubation was carried out with the degree of shaking being 140 per minute.

&Lt; Example 21 >

Proceeding in the same manner as in Example 2, incubation was carried out at 160 rpm for one minute.

&Lt; Example 22 >

Proceeding in the same manner as in Example 3, incubation was carried out at 120 rpm for one minute.

&Lt; Example 23 >

Proceeding in the same manner as in Example 3, incubation was carried out at 140 with one round of shaking.

&Lt; Example 24 >

Proceeding in the same manner as in Example 3, incubation was carried out at 160 rpm for one minute.

&Lt; Example 25 >

Proceeding in the same manner as in Example 8, the cultivation was continued with the degree of shaking being 120 per minute.

&Lt; Example 26 >

Proceeding in the same manner as in Example 8, incubation was carried out at 140 rpm for one minute.

&Lt; Example 27 >

Proceeding in the same manner as in Example 8, incubation was carried out at 160 rpm for one minute.

&Lt; Comparative Example 19 >

The procedure was carried out as in Example 2, except that the culture was carried out with 80 shakes per minute.

&Lt; Comparative Example 20 &

Proceeding in the same manner as in Example 2, incubation was carried out with 100 shaking degrees per minute.

&Lt; Comparative Example 21 &

Proceeding in the same manner as in Example 2, incubation was carried out with an order of 180 in one minute.

&Lt; Comparative Example 22 >

Proceeding in the same manner as in Example 3, culturing was carried out with 80 shaking degrees per minute.

&Lt; Comparative Example 23 >

Proceeding in the same manner as in Example 3, culturing was carried out with 100 shaking degrees per minute.

&Lt; Comparative Example 24 >

Proceeding in the same manner as in Example 3, incubation was carried out with the degree of shaking at 180 for one minute.

&Lt; Comparative Example 25 >

Proceeding in the same manner as in Example 8, the cultivation was carried out with 80 shaking degrees per minute.

&Lt; Comparative Example 26 >

Proceeding in the same manner as in Example 8, the culture was carried out with the degree of shaking at 100 per minute.

&Lt; Comparative Example 27 >

Proceeding in the same manner as in Example 8, culturing was carried out with an order of 180 in one minute.

Table 3 below shows the results of the cultivation conducted through Examples 19 to 27 and Comparative Examples 19 to 27.

<Table 3>

As shown in Table 3 above, it can be seen that the culture completion (++++) proceeds more rapidly in the culture conditions proceeding through Examples 19 to 27 of the present invention.

&Lt; Example 28 >

Proceeding in the same manner as in Example 2, culturing was carried out at an inoculation concentration of 10%.

&Lt; Example 29 >

The procedure was carried out in the same manner as in Example 2, but the culture was carried out at an inoculation concentration of 12.5%.

&Lt; Example 30 >

Proceeding in the same manner as in Example 4, the culture was carried out at an inoculation concentration of 10%.

&Lt; Example 31 >

The procedure was carried out in the same manner as in Example 4, except that the culture was carried out at an inoculation concentration of 12.5%.

&Lt; Example 32 >

Proceeding in the same manner as in Example 8, culturing was carried out at an inoculation concentration of 8.3%.

&Lt; Example 33 >

Proceeding in the same manner as in Example 8, culturing was carried out at an inoculation concentration of 10%.

&Lt; Example 34 >

Proceeding in the same manner as in Example 8, the culture was carried out at an inoculation concentration of 12.5%.

&Lt; Comparative Example 28 >

Proceeding in the same manner as in Example 2, the culture was carried out at an inoculation concentration of 6.6%.

&Lt; Comparative Example 29 &

Proceeding in the same manner as in Example 2, the culture was carried out at an inoculation concentration of 8.3%.

&Lt; Comparative Example 30 &

Proceeding in the same manner as in Example 4, the culture was carried out at an inoculation concentration of 6.6%.

&Lt; Comparative Example 31 >

Proceeding in the same manner as in Example 4, the culture was carried out at an inoculation concentration of 8.3%.

&Lt; Comparative Example 32 >

Proceeding in the same manner as in Example 8, the culture was carried out at an inoculation concentration of 6.6%.

Table 4 below shows the results of the cultivation conducted through Examples 28 to 34 and Comparative Examples 28 to 32.

<Table 4>

As shown in Table 4 above, it can be seen that the culture completion (++++) proceeds more rapidly in the culture conditions proceeding through Examples 28 to 34 of the present invention.

A total of 18 cultivations were carried out under the conditions of Example 1, and the dry matter content of the cultured bacterium, Cordyceps mellonus, was measured.

<Table 5>

As shown in Table 5 above, in the culture conditions under the conditions of Example 1 of the present invention, it can be seen that the mushroom mycelium of Bat moth is cultured in a large amount of dry matter.

A total of 16 cultivations were carried out under the conditions of Example 4, and the dry matter content of the cultured bacterium moth, Mycobacterium tuberculosis, in all components was measured and shown in Table 6 below.

<Table 6>

As shown in Table 6 above, in the culture conditions under the condition of Example 4 of the present invention, it can be seen that the mushroom mycelium of Bat moth is cultured in a large amount of dry matter.

A total of 17 cultivations were carried out under the conditions of Example 8, and the dry matter content of the cultured bacterium, Cordyceps mellonus, was measured.

<Table 7>

As shown in Table 7 above, in the culture conditions under the condition of Example 8 of the present invention, it can be seen that the mushroom mycelium of Bat moth is cultured in a large amount of dry matter.

In addition, the mangyide content and the moisture content of the Bat moth ciliothermia mycelium cultured through Example 1 were 8.2% and 5.6%, respectively. This is similar to Mandeed content of Chinese natural caterpillar fungus.

L-threonine, L-threonine, L-threonine, L-threonine, L-threonine, L-valthioate, L-valthio-L-thymandate, and L-valthio-L-thymandleptitide were detected. The polysaccharide content of the dried product was 48% to 65% of mildite, 22.13% of semi-lactose, 5.04% 2.73%.

In addition, erythrocytes, yopyrimidine thallium glycosides, adenopril oligosaccharides, yopyrimidine urate glycosides and crude glycosides were detected and erythrocyte erythrocytes, erythrocyte steroid peroxides, monosteroids and beta - globulins were detected.

The content of amino acids contained in the mycelial bacterium of Bat moth cultured through Example 1 is shown in Table 8 as compared with that of natural caterpillar fungus.

<Table 8>

As shown in Table 8 above, it can be seen that the content of amino acids in the mycelium of the Chinese cabbage cultivated through Example 1 of the present invention is much higher than that of natural Chinese cabbage.

The contents of trace elements contained in the mycelia of Bat moth caterpillar fungus cultured through Example 1 are shown in Table 9 below in comparison with natural caterpillar fungus.

<Table 9>

As shown in Table 9 above, it can be seen that the content of trace elements in the mycelium of Cordyceps sinensis cultured through Example 1 of the present invention is higher than that of natural cordyceps.

S101; Washing step
S103; Cordyceps specimens preparation stage
S105; Incubator manufacturing step
S107; Incubation step

Claims (8)

A washing step of washing moth caterpillars;
A step of preparing a Chinese caterpillar fodder for preparing Cordyceps sinensis by mixing the distilled water after polishing the Bat moth caterpillars undergoing the washing step;
An incubator preparation step in which a mixture containing grain powder, protein copper, glucose, potassium phosphate, magnesium sulfate and agar is heated and dissolved and then the dissolved mixture is poured on a glass surface to prepare an incubator and sterilization treatment; And
Culturing the bacterium Cordyceps sinensis produced in the step of preparing the bacterium of the Cordyceps sinensis by inoculating and culturing the bacteriocidal bacterium prepared in the step of producing the bacterium of the present invention.
The method according to claim 1,
Wherein the washing step comprises washing the moth caterpillar fungus with purified water, followed by washing with alcohol cotton.
The method according to claim 1,
In the step of preparing the Cordyceps specimens, the bat moth caterpillar fungus that has undergone the washing step is newly cut, the cross section of the cut cordyceps is dug out and put into the ground with the sea sand and ground. 100 parts by weight of distilled water is added to 100 parts by weight of the ground mixture, And then polishing and re-polishing the thus-obtained mycelia.
The method according to claim 1,
Wherein the content of the cereal flour is 3 to 5 parts by weight based on 100 parts by weight of the whole culture machine, 0.3 to 0.5 parts by weight of protein copper, 1 to 3 parts by weight of glucose, 0.05 to 0.2 parts by weight of potassium phosphate, 0.5 part by weight and 1 to 3 parts by weight of agar is dissolved by heating and then poured on a glass surface to prepare an incubator and sterilized.
The method according to claim 1,
Wherein the grain powder comprises one selected from the group consisting of wheat bran, sulfur nut, and potato starch.
The method of claim 4,
Wherein the sterilization treatment is performed by irradiating the incubator with ultraviolet rays for 30 minutes at a temperature of about 30 centimeters from the incubator at room temperature under high pressure sterilization for 30 minutes at a temperature of 120 캜 for 30 minutes.
The method according to claim 1,
Wherein the culturing step comprises inoculating the Cordyceps specimens prepared in the step of preparing Cordyceps sinensis with the culture medium prepared in the step of culturing the cells at a concentration of 8 to 12.5%, then the shaking degree is 120 to 160 times per minute, At a temperature of 5 to 7 days. &Lt; RTI ID = 0.0 &gt; 8. &lt; / RTI &gt;
A mycelium of moths, moths and caterpillars, characterized in that the moths are cultured by the method for culturing mycelium of moths of the moths according to any one of claims 1 to 7.

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