KR20130115481A - Skin external composition containing extracts of corylus heterophylla fisch. ex trautv - Google Patents

Abstract

PURPOSE: An external use skin composition containing a Corylus heterophylla Fisch. ex Trautv. extract is provided to enhance antioxidation, anti-aging, whitening, and moisturizing effects. CONSTITUTION: An external use skin composition contains 0.001-50 wt% of a Corylus heterophylla Fisch. ex Trautv. extract as an active ingredient. The composition is used for enhancing antioxidation, anti-aging, and anti-wrinkling effects and elasticity.

Description

Skin External Composition Containing Extracts of Corylus heterophylla Fisch. ex Trautv.}

The present invention relates to a topical skin composition containing a sachet extract, and more particularly, to a topical skin composition containing a sachet extract having an excellent effect on skin antioxidant, anti-aging, whitening and moisturizing.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness is reduced, wrinkles are increased, elasticity is reduced only In addition, the color of the skin becomes dull, skin troubles frequently occur, and blemishes, freckles, and mushrooms also increase, causing various changes.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are constituting skin, appear, and are subjected to oxidative stress caused by free radicals and active harmful oxygen. In addition, by aging or ultraviolet light, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation in most cells that make up the skin Increased biosynthesis, increased biosynthesis of matrix metalloproteinase (MMP), an enzyme that degrades skin tissue by these inflammatory factors, and increased nitric oxide (NO) production by inducible nitric oxide synthase (iNOS) It is known. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging. Therefore, a lot of research is being conducted on the active ingredient that can prevent and improve the phenomenon of aging.

On the other hand, free radicals generated by various physical, chemical and environmental factors such as enzymes, reducing metabolism, chemicals, pollutants and photochemical reactions in the body are non-selective and irreversible to cell components such as lipids, proteins, sugars and DNA. It has been known to cause various diseases including cell aging or cancer by performing a destructive action. In addition, various peroxides in the body, including lipid peroxides produced as a result of lipid peroxidation by these free radicals, also cause oxidative destruction of cells and cause various functional disorders. Accordingly, antioxidants such as free radical scavengers or peroxide production inhibitors are expected as agents for inhibiting or treating aging and various diseases caused by these oxides.

In the present invention, the bag ( Corylus heterophylla Fisch. ex Trautv. Or Corylus heterophylla var. thunbergii blum)

It is a deciduous shrub that is wild in Jeju Island. The bark is greyish brown, the leaves are alternate, ovate round or obovate, and the tip is pointed and fine teeth are on the edge. Lateral veins are 6-7 pairs, surface has fine hairs, but disappears, yellowish green on the back, hairs, and hairs on veins. Petiole 1-2cm long, hairy, with red pattern on young leaves. The bark is shiny grayish brown, and the new branch is brown with hairs. There has been no report on the effects of anti-aging, whitening and moisturizing on the skin on the sac.

Thus, the inventors have found that the sachet extract has excellent antioxidant, anti-aging, whitening and moisturizing effects when used as an external preparation for skin and completed the present invention.

Accordingly, it is an object of the present invention to provide a skin external preparation composition containing a sachet extract, which is excellent for skin antioxidant, anti-aging, whitening and moisturizing.

In order to achieve the above object, the present invention provides a skin external composition for antioxidant containing a sachet extract as an active ingredient.

The present invention also provides an anti-aging skin external composition comprising an extract of sachets as an active ingredient.

In addition, the present invention provides a skin external preparation composition for enhancing skin elasticity containing a sachet extract as an active ingredient.

In addition, the present invention provides a skin external preparation composition for improving skin wrinkles containing a sachet extract as an active ingredient.

The present invention also provides a whitening skin external composition comprising a sachet extract as an active ingredient.

In another aspect, the present invention provides a moisturizing skin external composition comprising a sachet extract as an active ingredient.

The external preparation composition for skin according to the present invention comprises a sacrum ( Corylus heterophylla Fisch. ex Trautv. Or Corylus heterophylla var. thunbergii BLUME) extract as an active ingredient.

The sachet extract of the present invention may be obtained according to a method known in the art, and for example, the sachet may be washed with purified water, dried by sun drying or hot air drying, and used as an organic solvent as an extracting solvent in the powdered powder. It may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and in consideration of the safety of the raw material, water or ethanol of 30 to 70% concentration is preferably used. The extract obtained by using the solvent in the extract is filtered after reflux extraction, concentrated under reduced pressure at 40 ~ 45 ℃ can be obtained as a dry sachet extract.

The composition of the present invention preferably contains the sachet extract in an amount of 0.001 to 50% by weight based on the total weight of the composition. This is because if the content of the sachet extract is less than 0.001% by weight, the efficacy and effect by the above ingredients is weak, and if the content of the sachet extract exceeds 50% by weight, there is a problem of skin stability or formulation.

The composition of the present invention can be used as an anti-skin preparation composition for antioxidant, and has an excellent antioxidant effect due to free radical scavenging or inhibition.

The composition of the present invention can be used as a composition for external application for skin for anti-aging, which is excellent in improving skin elasticity and improving wrinkles.

The composition of the present invention may be used as a moisturizing skin external preparation composition, which may enhance skin barrier function and induce differentiation of skin keratinocytes.

The composition of the present invention can be used as a composition for whitening, which can provide an excellent whitening effect by inhibiting tyrosinase activity and inhibiting melanin production.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non- It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In addition, the composition of the present invention, in addition to the sachet extract, other components that can give a synergistic effect to the skin protection effect preferably within the range that does not impair the antioxidant, anti-aging, moisturizing and whitening effect of the present invention. It may contain.

The present invention can provide a composition excellent in antioxidant, anti-aging, skin whitening and skin moisturizing effect by containing a sachet extract.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[ Manufacturing example  One] Sleeping bag  Preparation of extract

Fresh sac harvested in fresh state was washed with purified water, dried by hot air drying, and extracted with reflux by adding 1000 ml of 70% ethanol to 100 g of the granulated powder, filtered and concentrated under reduced pressure at 40∼45 ° C. 18 g was obtained.

[ Test Example  1] Antioxidant Effect Test ( DPPH  Test)

In order to examine the antioxidant effect of the sachet extract prepared in Preparation Example 1, to reduce the free radical DPPH (1,1-diphenyl-2-picrylhydrazyl; 1,1-diphenyl-2-picryl hydrazyl) The antioxidant activity was evaluated by comparing the DPPH oxidation inhibition effect through the change in absorbance generated by the antioxidant (oxidized by oxidizing agent). That is, the degree of inhibition of DPPH oxidation and the absorbance of the cyst extract obtained in Preparation Example 1 were measured, and the concentration showing an absorbance of 50% or less compared to the control group was evaluated as an effective antioxidant concentration. .

190 μl of 100 μM (in ethanol) DPPH solution and 10 μl of the sachet extract and the positive control of Preparation Example 1 were prepared, and the reaction solution was made. After reacting for 30 minutes at 37 ° C., the absorbance was measured at 540 nm. As a positive control, Trolox, a widely used synthetic antioxidant, was used. The DPPH analysis results of each substance are shown in Table 1 below, and IC ₅₀ means the sample concentration when the absorbance was reduced by 50% by the added sample.

Test substance IC ₅₀ (ppm) Trolox 43 Cumulus Extract (Manufacturing Example 1) 46

As can be seen in Table 1, the sachet extract of Preparation Example 1 has a similar efficacy effect compared to trolox, a synthetic antioxidant used as a positive control, the sachet extract according to the present invention was found to have an excellent antioxidant effect .

[ Test Example  2] anti-aging effect- Collagenase  Expression suppression effect

The collagenase production inhibitory ability of the sachet extract obtained in Preparation Example 1 was measured in comparison with tocopherol and EGCG. Tocopherol and EGCG are antioxidants and are known to have the function of regenerating the epidermal cells of the skin to prevent aging of the skin.

The test was performed by placing human fibroblasts at 5,000 cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. Incubate until 90% growth. After 24 hours of incubation in serum-free DMEM medium, 50 ppm concentration of sachet extract of Preparation Example 1, tocopherol and EGCG, respectively, dissolved in serum-free DMEM medium were treated at 10-4 molar concentration for 24 hours, followed by cell culture. Was taken.

The collected cell culture solution was measured for collagenase production using a commercially available collagenase measuring instrument (Amersham Pharma Co., Ltd., USA). First, the collected cell culture was placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-bound secondary collagen antibody was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the coloring stimulant was added, causing color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development). The color of the reaction solution was yellow and the degree of yellow color was different according to the progress of the reaction.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the cell culture obtained from the untreated group was used as a control. That is, the degree of expression of collagenase in the untreated group was set at 100, and the degree of expression of collagenase in the group treated with the test substance was determined, and the results are shown in Table 2 below.

Test substance Collagenase expression level (%) Untreated group 100 Tocopherol 76 EGCG 69 Cumulus Extract (Manufacturing Example 1) 72

The lower the expression level of collagenase, the higher the expression inhibiting ability of collagenase, less breakdown of collagen in the skin, inhibiting the decrease in skin elasticity, and the amount of wrinkles produced is smaller. As shown in Table 2, the sachet extract of the present invention effectively inhibited the expression of collagenase in vitro, and it was confirmed that the expression inhibiting ability of collagenase was superior to tocopherol and EGCG. That is, the sachet extract more effectively inhibits the expression of collagenase, thereby reducing collagen breakdown in the skin, and thus, it was found that the anti-aging effects such as skin elasticity enhancement and wrinkle reduction were excellent.

[ Test Example  3] whitening effect-inhibitory effect of melanin production by using pigment cells of rat

The melanin production inhibitory ability of the sachet extract obtained in Preparation Example 1 was measured by comparing with hydroquinone, a known whitening substance.

Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) in DMEM with 10% fetal placental serum, 100nM 12-O-tetradecanoyl Incubated at 37 ° C. with 5% CO 2 in medium containing phorbol (tetradecanoylphorbol) -13-acetate and 1 nM cholera toxin. The cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, the cells were incubated at a concentration of 105 cells / well in a 24-hole plate, and each test substance was added for 3 consecutive days from the second day. Incubated. As a test substance, hydroquinone and the sachet extract of Preparation Example 1 were each used at a concentration of 25 ppm. In this case, the hydroquinone was used as a positive control. Then, the culture medium was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and then the melanin production inhibition rate was calculated according to Equation 2 below and the results are shown in Table 3 below. Dooley's way).

Test substance Melanin production inhibition rate (%) Non-treated group 0 Hydroquinone (positive control) 48.2 Cumulus Extract (Manufacturing Example 1) 52.3

As shown in Table 3, the sachet extract of the present invention was confirmed to show a melanin production inhibition rate similar to that of hydroquinone, a known whitening substance, it can be seen that the whitening effect is excellent by effectively inhibiting melanin production.

[ Test Example  4] Skin Moisturizing Test-Differentiation Induction Test of Human Keratinocytes

Preparation Example 1 A test using absorbance was performed to confirm skin barrier function and skin moisturizing power of the sacrum extract.

Human neonatal keratinocytes (HEK) (Lonza, NHEK-Neo-Neonatal Normal Human Epidermal Keratinocytes, Pooled) were placed in a 6-well plate containing medium (KBM-gold) at 5x10 ⁴ cells / ball. After dispensing at (cells / well) and incubating for 24 hours at 37 ° C. and 5% CO ₂ , 50 ppm and 100 ppm concentrations of sachets were added to the culture solution, until the cells grew to 80-90% of the floor area. Incubated daily. The cells were harvested and washed with PBS (phosphate buffered saline), followed by 10 mM Tris-HCl (Tris-HCl) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). , pH 7.4) 1ml was added, and then boiled for 10 minutes after sonication (sonication) for 3 minutes. This was centrifuged at 1200 rpm for 30 minutes, and the separated precipitate was suspended in 1 ml of PBS and the absorbance at 340 nm was measured.

Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference when evaluating the degree of cell differentiation. The low calcium (0.03 mM) and high calcium (1.2 mM) treated groups were negative / positive controls, respectively, and the amount of CE (Cornified Envelop) produced during the differentiation of keratinocytes by adding a test substance at low calcium concentration. The results of comparing the cell differentiation promoting effect by measurement is shown in Table 4 below.

Test substance Concentration The ability to differentiate in keratinocytes (%) Control group Low Calcium Treated Group (0.03 mM) 100 High Calcium Treated Group (1.2 mM) 208 Cumulus extract (Production example 1) 50 ppm 125 100 ppm 163

As shown in Table 4, the sachet extract according to the present invention was found to enhance the barrier function of the skin and promote skin moisturizing ability by promoting differentiation in keratinocytes.

Claims (8)

Sleeping bag ( Corylus heterophylla Fisch. ex Trautv.) An external composition for skin containing the extract as an active ingredient. The composition for external application for skin according to claim 1, wherein the sachet extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. 3. The composition for external application for skin according to claim 1 or 2, wherein the composition is for antioxidant. The composition for external application for skin according to claim 1 or 2, wherein the composition is anti-aging. The composition for external application for skin according to claim 1 or 2, wherein the composition is for enhancing skin elasticity. The composition for applying the external of the skin according to claim 1 or 2, wherein the composition is for improving skin wrinkles. The composition for external application for skin according to claim 1 or 2, wherein the composition is for moisturizing the skin. The composition for applying the external of the skin according to claim 1 or 2, wherein the composition is for skin whitening.