JP2012144446A - Antioxidant and collagen production promotor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a plant derived novel antioxidant and collagen production promotor having excellent free radical scavenging power which prevents/inhibits skin ageing and skin diseases due to active oxygen (free radical) and excellent collagen production promotion.SOLUTION: The antioxidant and the collagen production promotor include an extract of Leucosceptrum stellipila (Miq.) in the genus Leucosceptrum.

Description

  The present invention relates to an antioxidant and a collagen production promoter, and more particularly to an antioxidant and a collagen production promoter containing a plant extract as an active ingredient.

  It is well known that active oxygen is generated by ultraviolet rays. Among active oxygens, free radicals react with an oxidizable substrate such as lipid to induce a chain oxidation reaction. Accordingly, active oxygen that becomes free radicals amplifies damage to body tissues such as skin.

  Since the skin is constantly exposed to oxygen and ultraviolet rays, it is the tissue with the greatest damage caused by oxidative stress due to free radicals. In recent years, various active oxygens generated by ultraviolet rays cause sebum and lipid peroxidation, protein denaturation, enzyme inhibition, etc., which in the short term induce skin inflammation and the like. In the long term, it is thought to cause aging and cancer.

Active oxygen and lipid peroxide are also considered to be involved in skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. Thus, active oxygen (free radicals and O ₂ ⁻ ) is deeply involved in skin aging and skin diseases.

A substance having the ability to capture free radicals and O ₂ ⁻ can suppress or stop the radical chain reaction, and for example, what is called an antioxidant is equivalent.
Therefore, an external preparation for skin containing an antioxidant can be expected to prevent or improve skin aging (for example, spots, wrinkles, sagging) due to photooxidative stress. In addition, prevention and improvement effects can be expected as a skin external preparation for various skin diseases related to free radicals.

  Vitamin E and vitamin C, which are known as antioxidants, are free radical scavenging antioxidants in vivo. In addition, synthetic antioxidants such as BHT and BHA are also known. In addition, as plant-derived antioxidants, extracts of shiitake, enokitake, shimeji, kawatake, matsutake, garlic mushroom, spinach, nameko, and other basidiomycetes have been reported (Patent Documents 1 to 3). Furthermore, an antioxidant composed of an extract of the genus Solanum spp. (Patent Document 4) and an antioxidant composed of an extract of the plant of the genus Pleurotus (Patent Document 5) have been reported.

On the other hand, the skin is composed of a stratum corneum, an epidermis layer, a basement membrane and a dermis from the outside, and the dermis is the widest part of the skin. Collagenous fibers, elastic fibers, glycoproteins, and proteoglycans have a heterogeneous structure in which they are spread in a three-dimensional manner, and each structure is in a gel state with a liquid phase. Collagen fibers are mainly composed of collagen, of which type I collagen accounts for 80% of the total. In addition to type I collagen, the presence of type III, IV, VII, IX and XII type collagen is known. Occurrence of wrinkles and sagging seen in aging skin is a major aging change in appearance, and is a serious problem for many middle-aged and elderly people. One of the causes of wrinkles and sagging is due to thinning of skin tissue with aging. In aging skin, there is a marked decrease in type I collagen fibers, the main matrix component of the dermis, and this is likely the main cause of skin thickness reduction.
Therefore, it is considered effective to prevent and improve wrinkles and sagging by promoting the production of type I collagen and maintaining the amount of type I collagen. Furthermore, promotion of type I and type III collagen production is also effective in improving wound healing of the skin.

  Conventionally, as an ingredient derived from natural products that promotes the production of type I and type III collagen to prevent and improve aging changes in the skin, for example, isoflavone compounds selected from daidzein, soy gin, genistein, and genistin, phytosterols (See Patent Document 6), a specific phytoplankton extract (see Patent Document 7) has been reported.

Patent Document 8 mentions an extract of trichomes as one of the plant extracts having a moisturizing action. The present invention relates to the antioxidant action and the type I collagen production promoting action of the licorice extract, but the description in Patent Document 8 and the contents of the present invention are completely different.
That is, although the moisturizing effect of Patent Document 8 is evaluated by the moisture remaining rate (%), wrinkles due to drying can be improved by a moisturizing agent that supplements moisture to the skin, and has been incorporated into many cosmetics. . On the other hand, wrinkles (crimp-like wrinkles) caused by a decrease in metabolism accompanying aging are mainly caused by a decrease in collagen production of dermal fibroblasts.

JP-A-5-317016 JP-A-6-65575 JP 59-124984 A JP-A-11-171723 Japanese Patent Laid-Open No. 11-171720 JP 2001-39849 A JP 2007-186471 A JP 2004-323406 A

From the above, it has been demanded to find a plant exhibiting a new antioxidant effect and collagen production promoting effect for plant-derived antioxidants and collagen production promoting agents.
The present invention has been made in response to such conventional circumstances, and an object thereof is to provide a novel plant-derived antioxidant and collagen production promoter having excellent antioxidant action and collagen production promoting action. .

  As a result of intensive studies in view of such a current situation, the present inventors have excellent antioxidant action and collagen production promoting action in specific plant extracts that have not been known to be effective in the past. As a result, the present invention has been completed.

  The present invention is an antioxidant comprising an extract of Leucosceptrum stellipila (Miq.).

  The present invention is a collagen production promoter characterized by comprising an extract of Leucosceptrum stellipila (Miq.).

The antioxidant of the present invention has an excellent antioxidant action, and has an excellent free radical scavenging ability to prevent / suppress skin aging and skin diseases caused by active oxygen (free radicals and O ₂ ⁻ ). is there.

  The collagen production promoter of the present invention is excellent in the effect of promoting collagen production and is safe. Therefore, according to the collagen production promoter of the present invention, collagen production can be promoted and the amount of collagen can be maintained, wrinkles and sagging can be prevented and improved, and skin wound healing can be promoted. It is effective in improving the symptoms.

It is a figure which shows the result of the SOD like activity of the licorice extract by this invention. It is a figure which shows the result of the DPPH radical scavenging activity of the licorice extract by this invention. It is a figure which shows the result of the collagen production promotion activity of the licorice extract by this invention.

Hereinafter, the present invention will be described in detail.
Leucosceptrum stellipila (Miq.), A plant used in the present invention, is also referred to as a turnback grass. It is.

  There has never been a report that the plant extract of the present invention has an antioxidant action or a collagen production promoting action, and both have been found for the first time by the present inventors.

  The extract of primrose used in the present invention can be obtained by drying or pulverizing leaves, stems or whole plants as they are, and then extracting them with a solvent. The extraction may be performed at room temperature, but the extraction can be promoted by heating, stirring and heating under reflux as necessary. The obtained extract can be used as it is or after being appropriately subjected to treatment such as filtration, concentration, and decolorization. It is also possible to remove the solvent once and redissolve it in a solvent different from the solvent used for extraction. The obtained extract can be further purified by activated carbon, column matography or the like.

The extraction solvent used in the present invention is not particularly limited as long as it is a solvent that is usually used for extraction, particularly alcohols such as methanol, ethanol, 1,3-butanediol, propylene glycol, and dipropylene glycol, hydrous ethanol, and hydrous methanol. Such water-containing alcohols, organic solvents such as acetone, ethyl acetate and chloroform can be used alone or in combination, but water-containing ethanol (for example, 50% ethanol) is particularly preferable.
These extracts may be used after being purified by solvent fractionation, activated carbon treatment, column chromatography, or the like.

  The antioxidant and the collagen production promoter of the present invention are characterized by containing an extract of Michaelis but can contain other various components as long as the effects of the present invention are not impaired.

  The antioxidant and collagen production promoter of the present invention can be blended in a skin external base to make a skin external preparation based on the antioxidant action and collagen production promotion action. These external preparations for skin can be suitably used particularly in the fields of cosmetics, pharmaceuticals, quasi drugs and the like.

  The blending amount of the plant extract in the external preparation for skin containing the antioxidant and the collagen production promoter of the present invention is usually 0.1 ppm or more, preferably 1 ppm or more, as a dry residue of the licorice extract component. If the blending amount is too small, the effect is not sufficiently exhibited. The upper limit is not particularly limited as long as the effect of the present invention is not impaired. However, even if it is excessively mixed, a remarkable effect commensurate with the increase in amount cannot be obtained, and there are cases where adverse effects are exerted on the formulation design and usability. Therefore, it is usually 10% by mass or less, more preferably 5% by mass or less.

  The skin external preparation containing the antioxidant and collagen production promoter of the present invention is produced by blending the antioxidant and collagen production promoter according to the present invention with a skin external base. In addition to the above essential components, the external preparation for skin is a component that is usually used for external preparations for skin such as cosmetics and pharmaceuticals, for example, moisturizer, antioxidant, oily component, ultraviolet ray, as long as the effects of the present invention are not impaired. Absorbers, surfactants, thickeners, alcohols, powder components, colorants, aqueous components, water, plant extracts, various skin nutrients, and the like can be appropriately blended as necessary.

  Others, disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine Other whitening agents such as various herbal medicines, drugs such as tocopherol acetate, glycyrrhizic acid and derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, alkoxysalicylic acid and / or salts thereof , Sugars such as glucose, fructose, mannose, sucrose, trehalose and the like can be appropriately blended.

  The external preparation for skin containing the antioxidant and the collagen production promoter of the present invention may be any ointment, cream, emulsion, lotion, pack, bath preparation, etc. as long as it is used for conventional external preparations for skin. It doesn't matter.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Unless otherwise specified, the amount is shown in mass%.
First, a method for preparing a plant extract used in this example, an antioxidant effect (reactive oxygen scavenging effect and radical scavenging effect), a test method for promoting collagen production, and the results thereof will be described.

1. Sample preparation (Production Example 1)
A 10-fold amount of 50% ethanol was added to 12 g of dried pulverized leaves and stems of trichomes (refining grass), extracted at room temperature for 1 week, left at 4 ° C. overnight, and filtered to remove insolubles. . The obtained filtrate was called 50% ethanol extract, and the solid substance obtained by freeze-drying was called 50% ethanol extract dry matter to obtain 2.5 g (yield; 21%).
(Production Example 2)
10 g of methanol was added to 12 g of dried pulverized leaves and stems of trichomes (refining grass) and extracted at room temperature for 1 week, left at 4 ° C. overnight, and filtered to remove insolubles. The obtained filtrate was referred to as “methanol extract”, and the solid obtained by freeze-drying was referred to as “methanol extract dry solid” to obtain 2.0 g (yield: 16%).

2. Method of measuring active oxygen scavenging action (SOD; superoxide dismutase-like activity) and its results (1) Principle This enzyme (SOD) is said to be a cause of accelerating skin aging (wrinkle generation, senile pigment spots, etc.) It is an enzyme that decomposes superoxide anion O ₂ ⁻ , which is one of the active oxygens, into hydrogen peroxide and oxygen. That is, nitrobrute erazolium is used as a method for detecting the superoxide anion, and the SOD-like activity of the test component is determined by determining the inhibition rate using a reduction color reaction for the decrease. Details are described below.

(2) Procedure As a coloring reagent, nitro blue tetrazolium (0.24 nmol / l) and xanthine 0.4 mmol / l are dissolved in 0.1 M phosphate buffer (pH 8.0), and as an enzyme stock solution, xanthine oxidase (Butter (derived from milk) 0.049 units / ml was dissolved in 0.1 M phosphate buffer (pH 8.0) and used. The blank solution was 0.1 M phosphate buffer (pH 8.0), and the reaction stop solution was sodium dodecyl sulfate (690 mmol / l).
The procedure was such that the extract dried product obtained in the production example was diluted with a 20% DIMSO (Dimethyl sulfoxide) solution to the respective concentrations, 10 μl was taken in a 96-well plate, and 100 μl of a coloring reagent was added and mixed. 100 μl of enzyme solution was added to this test, and 100 μl of blank solution was added to the blind test. After heating accurately at 37 ° C. for 28 minutes, the reaction was stopped by adding 20 μl of a reaction stop solution. The measurement was carried out using a plate reader at an absorbance of 560 nm. The number of specimens was 1 group n = 4 (hole).

(3) Calculation method SOD-like activity was calculated by applying the following equation.
SOD-like activity (% inhibition) = ((Ebl- (Ebl-bl))-(Es- (Es-bl))) / (Ebl- (Ebl-bl)) * 100
= 1- (Es- (Es-bl)) / (Ebl- (Ebl-bl)) * 100
Ebl: Absorbance at a wavelength of 560 nm of the solution obtained by adding the enzyme solution to the solution excluding the test sample
(Ebl-bl): Absorbance at a wavelength of 560 nm of a solution obtained by removing the enzyme solution from the solution obtained by removing the test sample
Es: Absorbance at a wavelength of 560 nm of a solution obtained by adding an enzyme solution to a test sample solution
(Es-bl): 560 nm absorbance of a solution obtained by removing the enzyme solution from the test sample solution

(4) Results The results are shown in FIG. FIG. 1 (a) is a graph showing the SOD-like activity of the methanol extract dried product prepared in Production Example 2, and FIG. 1 (b) is the SOD of the 50% ethanol extract dried product prepared in Production Example 1. It is a figure which shows like activity.
1 (a) and 1 (b), it was revealed that the SOD-like activity of the extract of licorice has activity in 50% ethanol extract dry matter and methanol extract dry matter.

3. Measurement method of radical scavenging action (DPPH; radical scavenging test method) and its results (1) Principle DPPH radical has an absorption wavelength in the visible region (517 nm), and the fact that the absorption wavelength disappears when reduced is tested. The radical scavenging ability of a substance is evaluated from the decrease in absorbance.

(2) Procedure Prepare a stock solution of 1 mM DPPH (1,1-diphenyl-2-picrylhydrazyl, Mw; 394.32) using DIMSO (Dimethyl sulfozide) and dilute it into a 0.1 mM DPPH solution at the time of use for evaluation. did. The test sample is diluted with a 20% DIMSO solution, dispensed in 10 μl increments of n = 4 (well) to a 96-well plate, immediately added with 90 μl of 0.1 mM DPPH, and allowed to react at room temperature for 10 minutes with gentle stirring. It was. The measurement was performed using a plate reader to measure the absorbance at 517 nm and evaluate the radical scavenging ability.

(3) Calculation method The radical scavenging activity was calculated by applying the following formula.
Radical scavenging rate (%) = ((Ct-Cb)-(St-Sb)) / (Ct-Cb) * 100
= 1- (St-Sb) / (Ct-Cb)) * 100
Ct: Absorbance at a wavelength of 517 nm of a solution obtained by adding DPPH to a solution excluding the test sample Cb: Absorbance at a wavelength of 517 nm of a solution obtained by removing DPPH from the solution excluding the test sample St; Solution obtained by adding DPPH to the solution of the test sample Absorbance at a wavelength of 517 nm of Sb; Absorbance at a wavelength of 517 nm of a solution obtained by removing DPPH from the test sample solution

(4) Results The results are shown in FIG. 2 shows the DPPH radical scavenging activity of the methanol extract dried product prepared in Production Example 2, and □ in FIG. 2 shows the DPPH radical of 50% ethanol extract dried product prepared in Production Example 1. It is a figure which shows erase activity.
As can be seen from FIG. 2, the DPPH activity of the 50% ethanol extract dry matter solution and the methanol extract dry matter solution of the licorice was significantly higher than that of the additive-free group.

4). Test method of collagen production promoting effect of fibroblasts and results (1) Procedure 4 million human skin-derived fibroblasts HF0 (K) P = 8.5 were cultured and seeded in 24 wells at 100,000 / hole. And 0.5% FBS / DME. The next day, the test sample methanol extract or 50% ethanol extract is added so that the concentration is 10 ppm and 100 ppm, and magnesium ascorbate is added so that the concentration is 0.25 mM. The concentration was adjusted to 0.5% FBS / DMEM. As a positive control, TGF-β (2 ng / μl) was used. After culturing for 3 days, the total collagen content of the culture supernatant was measured. The amount of type I collagen secreted into the culture supernatant was measured using enzyme immunosorbent assay (ELISA). That is, 100 μl of the standard antibody solution was added to the anti-PIP monoclonal antibody plate, and 20 μl of the standard solution prepared in advance was added to the culture supernatant and reacted at 37 ° C. for 3 hours. Discard the reaction solution, wash 4 times with PBS, add 100 μl each of substrate solution (3,3 ', 5,5' tetramethylbenzidine solution) and react at room temperature for 15 minutes to obtain antibody plate / PIP antigen / standard antibody. I made a conjugate. Next, 100 μl of a reaction stop solution in which 1N sulfuric acid was dissolved was added and stirred well to stop the reaction. Absorbance at 405 nm was measured with a microplate reader at 450 nm using distilled water as a control, and the amount of type I collagen was calculated. In addition, the number of cells was measured in order to evaluate the collagen production promoting action (type I collagen amount / cell number). The cell number measurement method is described below.

(2) Cell Counting Method (2-1) Procedure Three days after the addition of the test sample, the medium was removed by suction, washed with 5% FBS, 60 μl Hoechst was added to 12 ml Hoechst medium and stirred, and 500 μl was added. Added to 6-well plates. After reacting at 37 ° C. for 1 hour, the cells were transferred to a 96-well plate, and the number of cells was calculated using a microplate reader (excitation wavelength: 355 nm, measurement wavelength: 460 nm).

(2-2) Calculation method Collagen production promoting action was calculated using the following formula.
Collagen production promotion rate (%) = (type I collagen produced by cells added with test sample / number of cells in group added with test sample) ÷ (type I collagen produced by cells not added with test sample) / Number of cells in the group to which no test sample is added) × 100
In order to exclude an increase in collagen production due to cytotoxicity, the group with high cytotoxicity with a cell growth rate of less than 20% was excluded from the collagen production promotion data.

(3) Results The results are shown in FIG. FIG. 3 is a diagram showing the collagen production promoting action of the methanol extract dry matter solution prepared in Production Example 2 and the 50% ethanol extract dry matter solution prepared in Production Example 1.
Collagen production-promoting action of mint liquor in methanol extract and 50% ethanol extract is higher in 50% ethanol than in methanol, and 50% ethanol is added to 50% ethanol. Compared with no addition at 10 ppm and 100 ppm, a significant collagen production promoting effect was observed.

  From the above results, the extract of licorice of the present invention exhibits excellent antioxidant effect and collagen production promoting effect, and thus exhibits excellent antioxidant action and collagen production promoting action on human skin. Therefore, the plant extract can be blended in an external preparation to be used as an anti-aging agent or a therapeutic agent for various skin diseases that prevents skin aging and maintains a youthful and healthy skin state.

Below, the formulation example of the antioxidant and collagen production promoter by this invention of various dosage forms is demonstrated as a formulation example. Needless to say, the present invention is not limited by these formulation examples and is specified by the scope of claims.
The licorice extract content in each formulation was expressed as the amount of dry residue after the extraction solvent was removed.

Formulation Formula 1 (Lotion) Mass%
Trimethylglycine 1.0
Main Mine Oxidant Michaelis / 50% Ethanol Extract Dry Product 0.0001
Glycerin 1.0
1,3-butylene glycol 5.0
Sodium alginate 0.1
Ethyl alcohol 5.0
Polyoxyethylene polyoxypropylene decyl tetradecyl ether 0.2
Sodium hexametaphosphate Appropriate amount Citric acid Appropriate amount Sodium citrate Appropriate amount Phenoxyethanol Appropriate perfume Appropriate amount Purified water Residue

Formulation Example 2 (Lotion) Mass%
Main oxidizer Michaelis / methanol extract dry matter / 50% -1,3 butanediol substitution solution 0.1
Glycerin 2.0
1,3-butylene glycol 4.0
Polyoxyethylene methyl glucoside 1.0
PEG / PPG-14 / 7 dimethyl ether 3.0
Erythritol 1.0
Polyoxyethylene hydrogenated castor oil 0.5
Polyglyceryl diisostearate 0.3
Triethylhexanoin 0.3
EDTA 3 sodium appropriate amount citric acid appropriate amount sodium citrate appropriate amount phenoxyethanol appropriate amount purified water remainder

Formulation Example 3 (Lotion) Mass%
Tranexamic acid 1.0
4-methoxysalicylate potassium 1.0
Lipoic acid 0.1
Clam leaf extract 0.1
Hipotaurine 0.1
Clara extract 0.1
Tounin extract 0.1
Beech bud extract 0.1
Main Mine Oxidant Michaelis / 50% Ethanol Extract 0.01
Magnesium ascorbate phosphate 0.1
Thiotaurine 0.1
Green tea extract 0.1
Western mint extract 0.1
Iris Root Extract 1.0
Trimethylglycine 1.0
Glycerin 1.0
1,3-butylene glycol 5.0
Hydroxyethyl cellulose 0.05
Ethyl alcohol 5.0
Polyoxyethylene polyoxypropylene decyl tetradecyl ether 0.2
EDTA 3 sodium appropriate amount citric acid appropriate amount sodium citrate appropriate amount phenoxyethanol appropriate amount perfume appropriate amount purified water residue

Formulation Example 4 (milky lotion)
Dipotassium glycyrrhizinate 0.05
Tocopherol acetate 0.5
This collagen production promoter Mica Elisabeth / 50% ethanol extract dry matter 0.001
Sodium L-glutamate 0.05
Fennel extract 0.1
Yeast extract 0.1
Giant extract 0.1
Hydroxypropyl-β-cyclodextrin 0.1
Glycerin 6.0
1,3-butylene glycol 5.0
Polyoxyethylene methyl glucoside 3.0
Sunflower oil 1.0
Squalane 2.0
Isododecane 4.0
Dimethylpolysiloxane 3.0
Xanthan gum 0.1
Carboxyvinyl polymer 0.1
Acrylic acid / alkyl methacrylate copolymer 0.1
Ethyl alcohol 5.0
Potassium hydroxide Appropriate amount Sodium hexametaphosphate Appropriate amount Bengala Appropriate amount Yellow iron oxide Appropriate amount Ethylparaben Appropriate perfume Appropriate amount Purified water Residue

Formulation Example 5 (Daytime emulsion)
Dipotassium glycyrrhizinate 0.1
This antioxidant Michaelis percolate / methanol extract dry matter / 50% -1,3 butanediol substitution solution 0.1
Tocopherol acetate 0.1
1,3-butylene glycol 5.0
Squalane 0.5
Isododecane 10.0
Isohexadecane 25.0
Dimethylpolysiloxane 2.0
Polyoxyethylene / methylpolysiloxane copolymer 1.5
Trimethylsiloxysilicic acid 1.0
4-t-butyl-4'-methoxydibenzoylmethane 1.0
2-Ethylhexyl paramethoxycinnamate 5.0
Diparamethoxycinnamate mono-2-ethylhexanoate glyceryl 1.0
Silicone coated fine particle titanium oxide 4.0
Dimethyl distearyl ammonium hectorite 0.5
Spherical polyethylene powder 3.0
Talc 5.0
EDTA 3 sodium appropriate amount phenoxyethanol appropriate amount perfume appropriate amount purified water remainder

Formulation Example 6 (milky lotion)
D-arginine 0.1
Royal Jelly Extract 0.1
Yeast extract 0.1
Collagen production promoter Michaelisau / 50% -1,3-butanediol extract 1.0
Stearyl glycyrrhetinate 0.05
Tocopherol acetate 0.1
Acetylated sodium hyaluronate 0.1
Glycerin 5.0
Dipropylene glycol 7.0
Polyethylene glycol 1500 2.0
Liquid paraffin 7.0
Vaseline 3.0
Behenyl alcohol 1.0
Batyl alcohol 2.0
Jojoba oil 1.0
Stearic acid 0.5
Isostearic acid 0.5
Behenic acid 0.5
Tetra-2-ethylhexanoic acid pentaerythritol 3.0
Cetyl 2-ethylhexanoate 3.0
Glycerol monostearate 1.0
Polyoxyethylene glycerol monostearate 1.0
Carboxyvinyl polymer 0.15
Sodium hexametaphosphate Appropriate potassium hydroxide Appropriate methylparaben Appropriate fragrance Appropriate purified water Residual

Formulation Example 7 (milky lotion)
Ascorbic acid glucoside 1.5
Tranexamic acid 1.0
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
Collagen production promoter Micaeliso / 50% ethanol extract 0.01
Pantothenyl ethyl ether 0.1
Stearyl glycyrrhetinate 0.1
Glycerin 7.0
1,3-butylene glycol 5.0
Polyethylene glycol 20000 0.5
Vaseline 2.0
Jojoba oil 3.0
Squalane 2.0
Phytosteryl hydroxystearate 0.5
Behenyl alcohol 0.5
Batyl alcohol 0.2
Dimethylpolysiloxane 2.0
Tetra-2-ethylhexanoic acid pentaerythrit 0.1
Polyoxyethylene hydrogenated castor oil 1.0
Polyoxyethylene glyceryl isostearate 3.0
4-t-butyl-4'-methoxydibenzoylmethane 0.1
Diparamethoxycinnamic acid mono-2-ethylhexanoate glyceryl 0.1
Xanthan gum 0.1
Carboxyvinyl polymer 0.2
Ethanol 5.0
Potassium hydroxide Appropriate amount Sodium pyrosulfite Appropriate amount Sodium hexametaphosphate Appropriate amount EDTA 3 sodium Appropriate amount Yellow iron oxide Appropriate amount Paraoxybenzoate Appropriate amount Purified water Residue

Formulation Example 8 (Cream)
This collagen production promoter, Michaelis perforum / 50% ethanol extract 1.0
Potassium 4-methoxysalicylate 3.0
Propylene glycol 5.0
Glycerin 8.0
Stearic acid 2.0
Stearyl alcohol 7.0
Hydrogenated Lanolin 2.0
Squalane 5.0
2-Octyldodecyl alcohol 6.0
Polyoxyethylene cetyl alcohol ether 3.0
Glycerin monostearate ester 2.0
Potassium hydroxide appropriate amount ethyl paraben appropriate amount perfume appropriate amount ion-exchanged water remainder

Formulation Example 9 (Cream)
4-methoxysalicylate potassium 1.0
3-O-ethylascorbic acid 1.0
Collagen production promoter Michaelisau / 50% ethanol extract 0.1
Coenzyme Q10 0.03
Tranexamic acid 2.0
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
Pantothenyl ethyl ether 0.1
Stearyl glycyrrhetinate 0.1
Glycerin 7.0
1,3-butylene glycol 5.0
Polyethylene glycol 20000 0.5
Vaseline 2.0
Behenyl alcohol 0.5
Batyl alcohol 0.2
Squalane 2.0
Phytosteryl hydroxystearate 0.5
Jojoba oil 3.0
Tetra-2-ethylhexanoic acid pentaerythrit 1.0
Dimethylpolysiloxane 2.0
Polyoxyethylene glyceryl isostearate 1.5
Polyoxyethylene hydrogenated castor oil 1.0
Carboxyvinyl polymer 0.2
Xanthan gum 0.1
Ethanol 5.0
Sodium hexametaphosphate Appropriate amount Yellow iron oxide Appropriate amount EDTA3 sodium Appropriate amount Potassium hydroxide Appropriate amount Paraoxybenzoate Appropriate amount Purified water Residue

Formulation Example 10 (two-layer type daytime emulsion)
Tranexamic acid 2.0
4-methoxysalicylate potassium 1.0
Main oxidizer Michaelis / methanol extract dry matter / 50% -1,3 butanediol substitution solution 0.1
Dipotassium glycyrrhizinate 0.02
Glutathione 1.0
Thiotaurine 0.05
Clara extract 1.0
Dipropylene glycol 5.0
Dimethylpolysiloxane 5.0
Isohexadecane 25.0
Polyoxyethylene / methylpolysiloxane copolymer 2.0
Dimethyl distearyl ammonium hectorite 0.5
Butylethylpropanediol 0.5
2-Ethylhexyl paramethoxycinnamate 7.5
Trimethylsiloxysilicate 5.0
Spherical polyalkyl acrylate powder 5.0
Dextrin palmitate coated fine particle zinc oxide 15.0
EDTA3 sodium appropriate amount methylparaben appropriate amount phenoxyethanol appropriate amount perfume appropriate amount purified water remainder

Formulation Example 11 (Gel) Mass%
Potassium 4-methoxysalicylate 0.1
Nettle extract 0.1
Main oxidizer Michaelis / methanol extract dry matter / 50% -1,3 butanediol substitution solution 0.1
Dipotassium glycyrrhizinate 0.1
Ascorbic acid glucoside 2.0
Tocopherol acetate 0.1
Ogon Extract 0.1
Yukinoshita extract 0.1
Glycerin 2.0
1,3-butylene glycol 5.0
Polyethylene glycol 1500 3.0
Polyethylene glycol 20000 3.0
Agar powder 1.5
Xanthan gum 0.3
Acrylic acid / alkyl methacrylate copolymer 0.05
Cetyl octanoate 3.0
Dimethylpolysiloxane 5.0
Sodium hexametaphosphate Appropriate amount Dibutylhydroxytoluene Appropriate amount Yellow iron oxide Appropriate amount Citric acid Appropriate amount Sodium citrate Appropriate amount Sodium hydroxide Appropriate amount Phenoxyethanol Appropriate perfume Appropriate amount Purified water Residue

Claims (2)

  An antioxidant comprising an extract of Leucosceptrum stellipila (Miq.).   A collagen production promoter comprising an extract of Leucosceptrum stellipila (Miq.).

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