KR20130027598A - Cosmetic composition containing extracted rice wine lees for whitening effect - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a suljigemi(rice wine lees) extract with a whitening effect is provided to suppress tyrosinase. CONSTITUTION: A cosmetic composition for skin whitening contains a suljigemi extract as an active ingredient, which is a byproduct of makkoli(Korean rice wine). The suljigemi extract contains 0.1-10 wt% of active ingredients. A method for preparing the suljigemi extract comprises: a step of extracting 10g of a suljigemi sample in 45 ml of dichloromethane and 45 ml of methanol at room temperature for 48 hour; a step of adding distilled water and 100 ml of dichloromethane and shaking; a step of adding 100 ml of hexane and strongly shaking for 10 minutes to prepare a hexane layer; a step of adding 100 ml of methane and strongly shaking for 10 minutes to fraction a methane layer; a step of fractioning a butanol layer and an aqueous layer; and a step of concentrating the fraction by reducing the pressure.

Description

Cosmetic composition containing extracted sake lees extract with whitening effect {cosmetic composition containing extracted rice wine lees for whitening effect}

 The present invention relates to a cosmetic composition having a whitening effect of liquor forge, and in particular, it shows a whitening effect by showing tyrosinase inhibitory effect by liquor forge extract, and makes sake lees extract safe for skin as an active ingredient. It relates to a cosmetic composition for the purpose of skin beauty.

    As the recent well-being craze begins, interest in our nation's unique rice wine (makgeolli) is increasing, and the distinctive refreshing taste and low alcohol content of the liquor are entering a new turning point. Makgeolli is divided into two types: raw makgeolli and sterilized makgeolli. Sterilized makgeolli is heat-treated, so it can be stored for a long time, but it has the disadvantage of losing taste and aroma of makgeolli. Even if this refrigerated storage has a disadvantage of only about 10 days.

    Therefore, various methods to extend the shelf life of 'fresh makgeolli' are being studied, but there is a limit in the characteristics of manufacturing makgeolli of fermentation method using live bacteria.

Therefore, as a way to maximize the value of mass-produced rice wine through the 'makgeolli boom', the development of cosmetic materials using high value-added useful ingredients from by-products through fermentation of rice wine is urgently needed.

In this development, we extracted whitening useful ingredients from fermentation by-products of makgeolli and developed high value-added cosmetic materials to promote makgeolli industry.

Human skin protects the human body from ultraviolet rays. The effect of UV radiation on the skin is responsible for the production of free radicals that are harmful to the skin, resulting in cell damage and, consequently, for aging of the skin.

   A protective action to inhibit the production of these harmful free radicals in the skin is melanization, which reduces the damage of ultraviolet rays in the skin. However, despite this positive role, over-produced melanin has been the cause of women's major concerns, such as blemishes and freckles. Inhibiting the production of melanin is the mainstream of whitening cosmetic research, it is made in three directions to suppress melanin production in melanocytes, control the melanocyte stimulant, and promote melanin excretion. Inhibiting melanin production involves controlling copper (Cu), an essential component of the active site of tyrosinase, an enzyme involved in melanogenesis, or controlling iron (Fe) ions, an essential component of tyrosinase-related proteins. The development of a substance that inactivates or inhibits the production of melanin intermediates is the main, kojic acid (arbutin), vitamin C derivatives, rucinol (rucinol) is known as a representative whitening agent. However, kojic acid has been discontinued due to the problem of skin residue safety, and arbutin and vitamin C derivatives have been raised due to physical stability and low activity. Therefore, the development of natural whitening cosmetic ingredients with excellent skin safety and whitening activity Desperate

   Therefore, through this development, we tried to develop a whitening cosmetic material using the by-product of makgeolli, a traditional liquor that is known to contain whitening active ingredients.

  Traditionally, 'the hands of people working in the brewery are white and clean' is a testament to the fact that rice wine contains tyrosinase inhibitors that have high skin safety with whitening effects.

   Unlike alcoholic liquor, makgeolli contains a large amount of sugar, vitamin B group and protein, and contains a large amount of free amino acid and bioactive substances, which are degradation products of yeast, as well as nutritional value. Also attracted attention as.

It is an object of the present invention to provide a cosmetic composition for skin whitening using the active ingredient having a whitening effect while showing tyrosinase inhibitory activity in various fermentation products of sake lees discarded after manufacturing makgeolli.

In order to achieve the above object, the present invention provides a cosmetic composition having a tyrosinase inhibitory activity with the sake lees extract as an active ingredient. Hereinafter, the present invention will be described in detail.

 The present invention provides a cosmetic composition exhibiting a whitening effect mixed in 5 to 10% by weight of liquor fork extract.

The composition is characterized in that it is formed to inhibit the function of tyrosinase enzyme.

The liquor fork extract is characterized in that it contains 0.1 to 10.0% by weight based on the total cosmetic composition.

As can be seen in the examples to be described below, the skin whitening liquor forge extract contained as an active ingredient of the present invention has an effect of showing tyrosinase inhibitory activity significantly on the existing ascorbic acid.

     Example 1: Preparation of liquor fork extract of the present invention

       The liquor forge was used to bring the liquor forge which was discarded after the production of Busan Geumjeong Makgeolli, and the sample was used to remove water and alcohol by dehydration and lyophilization of the liquor forge.

10 g of the liquor forge sample was placed in a 300 ml volumetric flask, and 45 ml of dichloromethane and 45 ml of methanol were added and extracted at room temperature for 48 hr. D.W and 100 ml of dichloromethane were added and stirred with shaking.

100 ml of hexane was added to the sample and the mixture was shaken vigorously for 10 minutes to fractionate the hexane layer. Next, 100 ml of methanol was added thereto and the mixture was shaken vigorously for 10 minutes to separate the methanol layer. Then, the butanol layer and the aqueous layer were fractionated in the same manner as above, and the fractions were concentrated under reduced pressure using a rotary evaporator, respectively, to confirm the whitening activity by evaluating the tyrosinase inhibitory activity of the sample.

Example 2 Emulsion Preparation

   Emulsion preparation produced emulsions with component and composition ratios as described in the table below. Specifically, the ingredients 1 to 12 described in the table, and heated to 80 degrees, then added purified water and slowly cooled to 40 degrees while stirring at 3,000 RPM, and then adjusted the pH to 5.5 with citric acid. After adding the fragrance and cooled to room temperature it was prepared.

 Ingredient Name%

 1 polysiloxane 5.0

 2 squalene 5.0

 3 cetearyl alcohol 2.0

 4-glycerylstearate 1.5

 5 sorbitan stearate 1.0

 6 Mystic Acid 1.0

 7 stearic acid 0.5

 8 Glycerin 3.0

 9 Butylene Glycol 5.0

10 Methylgluses 1.0

11 Dimethicone Polymer 0.5

12 Methylparaben 0.1

13 remaining amount of purified water

23 Spices 0.1

Example 3: Evaluation of Tyrosinase Inhibitory Activity of Alcoholic Forge Fractions

 According to the method of Yagi et al. In other words, 0.2mL of 110Unit / mL mushroom tyrosinase was added to the mixed solution containing 0.2mL of the substrate solution dissolved in 10mM L-DOPA in 0.5mL of 1 / 15M sodium phosphate buffer at pH 6.8 and 0.1mL of the sample solution. DOPA chrome produced in the reaction solution was measured before and after the reaction at 475 nm.

Tyrosinase inhibitory activity was expressed by the absorbance decrease rate of the sample solution addition and no addition groups.

Tyrosinase inhibition activity (%) = (1-DOPA chrome production in the reaction group / DOPA chrome production in the control group)

Sample concentration (%) One 0.1 0.01 Tyrosinase Inhibitory Activity Training control Ascorbic acid 96.4 94.7 90.5 Solvent extraction Hexane Layer 32.3 22.5 17.2 MeOH  layer 88.6 74.5 55.8 Butanol Layer 22.2 12.5 5.8 Water layer 30.2 21.8 15.3

As a result of examining tyrosinase inhibitory activity, tyrosinase inhibitory activity was shown to be 78 ~ 90% of Ascorbic acid (0.1 ~ 1%), which is the positive control in MeOH fraction of sake lees. .

Claims (2)

Cosmetic composition for skin whitening using sake lees extract, a byproduct of makgeolli, as an active ingredient
The cosmetic composition for skin whitening according to claim 1, wherein the ratio of the liquor forge extract active ingredient is 0.1 to 10% by weight.