KR20130002943A - Composition for antiaging comprising extract or fraction of ardisia tinctoria pit. as an active ingredient - Google Patents
Composition for antiaging comprising extract or fraction of ardisia tinctoria pit. as an active ingredient Download PDFInfo
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- KR20130002943A KR20130002943A KR1020120067900A KR20120067900A KR20130002943A KR 20130002943 A KR20130002943 A KR 20130002943A KR 1020120067900 A KR1020120067900 A KR 1020120067900A KR 20120067900 A KR20120067900 A KR 20120067900A KR 20130002943 A KR20130002943 A KR 20130002943A
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- KR
- South Korea
- Prior art keywords
- fraction
- extract
- aldicia
- tincture
- aging
- Prior art date
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Images
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
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Abstract
Description
본 발명은 노화방지용 조성물에 관한 것으로, 구체적으로 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 화장료 조성물, 약학적 조성물 및 건강식품용 조성물에 관한 것이다.
The present invention relates to an anti-aging composition, specifically Aldicia tincture ( Ardisia) tinctoria It relates to an anti-aging cosmetic composition, a pharmaceutical composition and a composition for health food containing an extract or a fraction thereof as an active ingredient.
인체의 노화와 질병을 유발하는 자유 라디칼은 인체 내에서 정상적인 대사과정 중 생물학적 반응으로 형성되며, 세포와 조직에 해로운 독성을 일으켜 여러 가지 질병을 유발하는 것으로 알려져 있다(Folin O & Denis W, 1912, J Biol Chem 12: 239-249). 이러한 유해 자유라디칼을 억제하는 생리작용으로는 산화성 자유라디칼에 전자를 공여하여 산화를 억제하는 전자공여능과 수퍼옥사이드 디스뮤타제(superoxide dismutase; SOD)와 유사한 역할로서 반응성과 파괴성이 매우 큰 수퍼옥사이드 음이온 라디칼(superoxide anion radical)을 정상상태의 산소로 전환 시켜주는 역할이 있다. 강력한 항산화 효과를 가지는 여러 가지 생리활성 성분들은 산화적 스트레스에 의해 발생되는 궤양과 경련의 예방, 위장관의 위액 분비조절과 설사예방, 당뇨예방, 암, 심장병 및 퇴행성 질병들의 예방과 감소에 크게 기여한다고 보고되고 있다(Azuma K et al., 1999, J Agric Food Chem 47: 3963-3966, Ham SS et al., 1997, J Food Sci Nutr 2:155-161). 그리고, 최근 건강에 대한 관심이 높아지면서 다양한 식물자원에서 항암, 항알러지, 항비만, 항산화 및 항균 등의 효과가 있는 기능성 물질을 다량 함유하는 자원을 선발하여, 약으로, 식품첨가제로, 또는 화장품의 원료로 개발하려는 연구가 전 세계적으로 진행되고 있다(Shin DB, et al., 2006, Food Sci Biotechnol., 15: 357-362, Park et al., 2004, Korean J Nutr, 37: 364-372, Kim et al., 2006, Kor J Food Sci. Technol. 38: 799-804, Ali KA et al., 2005, Food Chem., 89: 27-36).
Free radicals that cause aging and disease in the body are formed by biological reactions during normal metabolic processes in the human body, and are known to cause various diseases by causing harmful toxicity to cells and tissues (Folin O & Denis W, 1912, J Biol Chem 12: 239-249). The physiological action of inhibiting such harmful free radicals is a superoxide anion that is highly reactive and destructive as it has a role similar to that of a superoxide dismutase (SOD) that provides electrons to oxidative free radicals to inhibit oxidation. It is responsible for converting radicals (superoxide anion radicals) into normal oxygen. Many bioactive ingredients with powerful antioxidant effects contribute greatly to the prevention of ulcers and spasms caused by oxidative stress, control of gastric juice secretion and diarrhea, prevention of diabetes, prevention of cancer, heart disease and degenerative diseases. It is reported (Azuma K et al ., 1999, J Agric Food Chem 47: 3963-3966, Ham SS et al ., 1997, J Food Sci Nutr 2: 155-161). In recent years, as interest in health has increased, a variety of plant resources have been selected to contain a large amount of functional substances, such as anti-cancer, anti-allergy, anti-obesity, antioxidant, and antibacterial, as medicines, food additives, or cosmetics Research is underway around the world to develop raw materials from raw materials (Shin DB, et. al ., 2006, Food Sci Biotechnol., 15: 357-362, Park et al ., 2004, Korean J Nutr, 37: 364-372, Kim et. al ., 2006, Kor J Food Sci. Technol. 38: 799-804, Ali KA et al ., 2005, Food Chem., 89: 27-36).
항산화제인 수퍼옥사이드 디스뮤타제(superoxide dismutase; SOD)는 세포의 호흡과정에서 발생한 활성산소 즉, 수퍼옥사이드 라디칼을 과산화수소와 산소로 분해시킨다. 과산화수소는 철이나 동이온과 같은 금속이온과 결합하여 반응을 하게 되면 하이드록시 라디칼이라는 보다 강력한 활성산소가 발생하게 된다. 과산화수소가 금속이온과 반응하기 전에 이를 제거해주는 항산화제인 카탈라아제와 글루타치온 퍼옥시다제가 과산화수소를 물과 이산화탄소로 분해를 시켜준다. 과산화수소를 제거해주는 항산화제가 5%의 과산화수소를 완벽하게 제거를 못 한 나머지 이것이 체내의 금속이온에 반응하여 하이드록시 라디칼로 변해 우리의 몸을 노화시키고 병들어 죽게 하는 것이다. 방사선이나 자외선의 잦은 노출은 과산화수소를 하이드록시 라디칼로 변하게 하여 노화를 촉진시키는데, 이를 지연시키는 방법으로는 항산화제의 복용이 있다. 수퍼옥사이드 라디칼(Superoxide radical, O2 -) 자체는 반응성이 비교적 약하지만, 쉽게 H2O2로 변환되어 결국, 반응성이 강한 하이드록실 라디칼을 생성하거나, 나이트릭 옥사이드(nitric oxide, NO)와 반응하여 반응성이 강한 퍼옥시나이트라이트(peroxy nitrite, ONOO)를 생성하여 SH-그룹의 산화, 단백질 티로신의 질산화(nitration), 지질과산화, DNA 손상 등을 일으키는 원인이 된다.Superoxide dismutase (SOD), an antioxidant, breaks down free radicals, ie superoxide radicals, generated during the respiration of cells into hydrogen peroxide and oxygen. When hydrogen peroxide reacts with metal ions such as iron or copper ions, it generates more powerful active oxygen called hydroxy radicals. The antioxidants catalase and glutathione peroxidase, which remove hydrogen peroxide before it reacts with metal ions, decompose hydrogen peroxide into water and carbon dioxide. Antioxidants that remove hydrogen peroxide do not completely remove 5% of hydrogen peroxide, which reacts with metal ions in the body, turning into hydroxy radicals, aging our bodies and causing them to die. Frequent exposure to radiation or ultraviolet light converts hydrogen peroxide into hydroxy radicals, which accelerates aging, which can be delayed by taking antioxidants. Superoxide radicals (Superoxide radical, O 2 -) itself is reactive is relatively weak, but easily is converted to H 2 O 2 As a result, generation of hydroxyl radicals strong reactivity, or age trick oxides (nitric oxide, NO) and the reaction It generates highly reactive peroxy nitrite (ONOO), causing oxidation of SH-group, nitrification of protein tyrosine, lipid peroxidation and DNA damage.
현재 항산화능을 측정하기위한 보편적인 방법으로는 biomarker인 자유라디칼의 홀전자가 수산기(-OH)와 반응하여 탈색되는 현상을 이용한 DPPH 혹은 ABTS 자유라디칼 소거 실험법을 많이 이용하고 있다.
Currently, DPPH or ABTS free radical scavenging method is widely used as a universal method for measuring antioxidant ability, in which hole electrons of free radicals, which are biomarkers, react with hydroxyl groups (-OH) and decolorize.
항산화 물질은 동·식물계에 널리 분포되어 있는데, 과일과 채소에 많은 페놀성 화합물, 플라보노이드, 토코페롤, 비타민 C, 셀레늄과 같은 항산화 물질은 지방의 산화를 지연시키거나 방지하며 암 및 심장혈관계 질환 등을 예방, 지연시킴으로써 노화방지에도 중요한 역할을 한다. 식물계에 존재하는 천연 항산화 물질인 토코페롤과 비타민 C는 식품, 의약품 및 화장품 등에 널리 이용되고 있다. 유지 또는 유지함유 식품의 산패는 주로 공기 중의 산소와 결합에 의해 일어나는데, 이를 방지하기 위하여 많은 합성 또는 천연 항산화 물질이 개발되어 왔으나, 그 효과와 경제성 때문에 실제로 많이 사용되고 있는 것은 합성 항산화제이다. 합성 항산화제로는 BHT(Butylated Hydroxy Toluene), BHA(Butylated Hydroxy Anisole), 프로필갈레이트(Propyl Gallate) 등이 있다. 합성 항산화제는 항산화력이 뛰어나고 가격이 저렴하여 상업용 식품에 많이 사용되고 있으나, 인체 부작용 등 안정성에 대한 우려로 그 사용량이 법적으로 규제되어 있다. 토코페롤과 같은 천연 항산화제는 인체에 대하여 안전하기는 하나 단독으로는 산화 연쇄반응 저지 능력이 있고, 가격이 비싼 단점이 있다. 따라서, 최근에는 보다 안전하고 항산화 효과가 뛰어난 천연 항산화제를 개발하기 위한 연구가 활발히 이루어지고 있다.
Antioxidants are widely distributed in animals and plants. Antioxidants such as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium in fruits and vegetables can delay or prevent the oxidation of fats and prevent cancer and cardiovascular diseases. Preventing and delaying plays an important role in preventing aging. Tocopherols and vitamin C, which are natural antioxidants present in the plant kingdom, are widely used in food, medicine, and cosmetics. The rancidity of fats and oils or foods containing oils and fats is mainly caused by the combination with oxygen in the air. To prevent this, many synthetic or natural antioxidants have been developed. Synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are widely used in commercial foods because of their excellent antioxidant power and low price, but their usage is legally regulated due to concerns about stability, such as human side effects. Natural antioxidants such as tocopherol, although safe for the human body, have the ability to block oxidative chain reactions alone, and are expensive. Therefore, in recent years, researches are being actively conducted to develop safer and more natural antioxidants.
한편, 알디시아 팅크토리아(Ardisia tinctoria Pit.)는 Myrsinaceae과에 속하는 식물이며, 민간에서 검정색 염료로 사용되었다고 알려져 있다. 동속식물인 A. crenata , A. pusilla의 경우 항산화활성이 있으며(2008-001634, Japan Patent), A.elliptica 식물에서 분리된 시랑산(syringic acid), 이소람테틴(isorhamnetin), 퀘세틴(quercetin)등의 성분들이 항균활성(살모넬라)을 갖는다는 연구결과(Methin Phadungkit & Omboon Luanratana, 20(7), 693-696, 2006, Natural Product Research) 등 동속식물에 대한 연구결과는 다양한 반면, 알디시아 팅크토리아에 대한 연구나, 성분에 대한 연구는 전혀 보고된 바가 없다.
On the other hand, Ardisia tinctoria Pit. Is a plant belonging to the family Myrsinaceae and is known to be used as a black dye in folklore. The same plant A. crenata and A. pusilla have antioxidant activity (2008-001634, Japan Patent), syringic acid isolated from A.elliptica plants, isorhamnetin , quercetin ) And other studies on homologous plants such as (Methin Phadungkit & Omboon Luanratana, 20 (7), 693-696, 2006, Natural Product Research) have a variety of results. No studies of tincture or components have been reported.
이에, 본 발명자들은 항산화 효과가 우수한 식물을 스크리닝하던 중, 알디시아 팅크토리아의 추출물 및 분획물이 우수한 DPPH(1,1-diphenyl-2-picryl-hydrazyl) 자유라디칼 소거 활성, ABTS(2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)) 자유라디칼 소거 활성을 나타내어 탁월한 항산화 효과를 가지고 있음을 확인함으로써, 노화방지용 조성물의 유효성분으로 유용하게 사용될 수 있음을 밝힘으로써 본 발명을 완성하였다.
Accordingly, the present inventors, while screening plants with excellent antioxidant effect, DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity, ABTS (2,2 ') with excellent extracts and fractions of aldicia tincture -azinobis- (3-ethylbenzothiazoline-6-sulphonic acid)) The free radical scavenging activity was confirmed to have an excellent antioxidant effect, thereby completing the present invention by revealing that it can be usefully used as an active ingredient of the anti-aging composition. .
본 발명의 목적은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 화장료 조성물, 약학적 조성물 및 건강식품용 조성물을 제공하는 것이다.
It is an object of the present invention to provide an anti-aging cosmetic composition, a pharmaceutical composition and a composition for health food containing Ardisia tinctoria Pit. Extract or a fraction thereof as an active ingredient.
상기 목적을 달성하기 위하여, 본 발명은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides an anti-aging cosmetic composition containing Aldisia tinctoria Pit. Extract or a fraction thereof as an active ingredient.
또한, 본 발명은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 약학적 조성물을 제공한다.In addition, the present invention Aldisia tincture ( Ardisia) It provides an anti-aging pharmaceutical composition containing tinctoria Pit.) extract or a fraction thereof as an active ingredient.
아울러, 본 발명은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 건강식품용 조성물을 제공한다.
In addition, the present invention Aldicia tincture ( Ardisia) It provides an anti-aging health food composition containing tinctoria Pit.) extract or a fraction thereof as an active ingredient.
본 발명의 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물은 DPPH 자유라디칼 생성 억제 효과, ABTS 자유라디칼 생성 억제 효과를 나타내어 우수한 항산화 효과를 가지고 있음을 확인함으로써, 노화방지용 화장료 조성물, 약학적 조성물 및 건강식품용 조성물의 유효성분으로 유용하게 사용될 수 있다.
Aldisia tincture of the present invention ( Ardisia) tinctoria Pit.) Extracts or fractions thereof exhibited an inhibitory effect on DPPH free radical production and ABTS free radical production, and confirmed that they have excellent antioxidant effects. As an active ingredient of anti-aging cosmetic composition, pharmaceutical composition and health food composition, It can be usefully used.
도 1은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물 제조과정을 나타낸 그림이다.
도 2는 알디시아 팅크토리아 추출물 또는 이의 분획물의 TLC(Thin Layer Chromatography)를 나타낸 그림이다:
T: 알디시아 팅크토리아 추출물;
H: 알디시아 팅크토리아 n-헥산 분획물;
C: 알디시아 팅크토리아 클로로포름 분획물;
E: 알디시아 팅크토리아 에틸아세테이트 분획물;
B: 알디시아 팅크토리아 부탄올 분획물; 및
W: 알디시아 팅크토리아 물 분획물.
도 3은 알디시아 팅크토리아 추출물 또는 이의 분획물의 CAD(Charge Aerosol Detector)를 나타낸 그림이다.
도 4는 알디시아 팅크토리아 추출물의 자유라디칼 소거 활성을 나타낸 그래프이다.
도 5는 알디시아 팅크토리아 추출물의 ABTS 소거활성에 대한 측정결과를 나타낸 그래프이다.1 is an Ardisia tincture tinctoria Pit.) Figure shows the process of extract or fractions thereof.
Figure 2 is a diagram showing Thin Layer Chromatography (TLC) of Aldicia tincture extract or fractions thereof:
T: aldicia tincture extract;
H: aldicia tinctureria n-hexane fraction;
C: aldicia tinctureria chloroform fraction;
E: aldicia tinctureria ethyl acetate fraction;
B: aldicia tinctureria butanol fraction; And
W: Aldisia tinktoria water fraction.
Figure 3 is a diagram showing the CAD (Charge Aerosol Detector) of the aldicia tincture extract or fractions thereof.
Figure 4 is a graph showing the free radical scavenging activity of the aldicia tincture extract.
Figure 5 is a graph showing the measurement results for ABTS scavenging activity of the aldicia tincture extract.
이하, 본 발명을 상세히 설명한다.
Hereinafter, the present invention will be described in detail.
본 발명은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 화장료 조성물을 제공한다.The present invention Aldicia tincture ( Ardisia) It provides an anti-aging cosmetic composition containing tinctoria Pit.) extract or a fraction thereof as an active ingredient.
상기 노화는 피부노화인 것이 바람직하나 이에 한정하지 않는다.The aging is preferably skin aging but is not limited thereto.
또한, 본 발명은 알디시아 팅크토리아(Ardisia tinctoria Pit.) 추출물 또는 이의 분획물을 유효성분으로 함유하는 항산화용 화장료 조성물을 제공한다.
In addition, the present invention Aldisia tincture ( Ardisia) tinctoria Pit.) It provides an antioxidant cosmetic composition containing an extract or a fraction thereof as an active ingredient.
상기 알디시아 팅크토리아 추출물은 하기의 단계들을 포함하는 제조방법에 의해 제조되는 것이 바람직하나 이에 한정되지 않는다:The aldicia tincture extract is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:
1) 알디시아 팅크토리아(Ardisia tinctoria)에 추출용매를 가하여 추출하는 단계;1) extracting by adding an extraction solvent to Ardisia tinctoria ;
2) 단계 1)의 추출물을 여과하는 단계; 및2) filtering the extract of step 1); And
3) 단계 2)의 여과한 추출물을 감압 농축한 후 건조하여 알디시아 팅크토리아의 추출물을 제조하는 단계.3) concentration of the filtered extract of step 2) under reduced pressure and drying to prepare an extract of aldicia tincture.
상기 방법에 있어서, 단계 1)의 알디시아 팅크토리아(Ardisia tinctoria)는 재배한 것 또는 시판되는 것 등 제한 없이 사용할 수 있다. 상기 알디시아 팅크토리아는 잎, 줄기 또는 뿌리가 모두 이용가능하나 이에 한정하지 않는다.In the method, Ardisia tincture ( Ardisia ) of step 1) tinctoria ) can be used without limitation, such as grown or commercially available. The aldicia tincture is available in all but not limited to leaves, stems or roots.
상기 방법에 있어서, 상기 단계 1)의 추출용매는 물, 알코올 또는 이들의 혼합물을 사용하는 것이 바람직하다. 상기 알코올로는 C1 내지 C2 저급 알코올을 이용하는 것이 바람직하며, 저급 알코올로는 에탄올 또는 메탄올을 이용하는 것이 바람직하고, 메탄올을 이용하는 것이 가장 바람직하나, 이에 한정하지 않는다. 추출방법으로는 진탕추출, Soxhlet 추출 또는 환류 추출을 이용하는 것이 바람직하나 이에 한정되지 않는다. 상기 추출용매를 건조된 알디시아 팅크토리아 분량에 1 내지 10배 첨가하여 추출하는 것이 바람직하고, 2 내지 3배 첨가하여 추출하는 것이 더욱 바람직하나, 이에 한정하지 않는다. 추출온도는 20 내지 100℃인 것이 바람직하고, 20 내지 40℃인 것이 바람직하나, 이에 한정하지 않는다. 또한, 추출시간은 10 내지 48시간인 것이 바람직하며, 15 내지 30시간인 것이 더욱 바람직하나 이에 한정하지 않는다. 아울러, 추출 회수는 1 내지 5회인 것이 바람직하며, 3 내지 4회 반복 추출하는 것이 더욱 바람직하나 이에 한정되는 것은 아니다. In the above method, the extraction solvent of step 1) is preferably water, alcohol or a mixture thereof. As the alcohol, C 1 to C 2 lower alcohols are preferably used, and as lower alcohols, ethanol or methanol is preferably used, and methanol is most preferably used, but not limited thereto. As the extraction method, it is preferable to use shaking extraction, Soxhlet extraction or reflux extraction, but is not limited thereto. The extraction solvent is preferably extracted by adding 1 to 10 times to the dried aldicia tincture amount, more preferably 2 to 3 times to extract, but not always limited thereto. The extraction temperature is preferably 20 to 100 ° C, preferably 20 to 40 ° C, but is not limited thereto. In addition, the extraction time is preferably 10 to 48 hours, more preferably 15 to 30 hours, but is not limited thereto. In addition, the extraction number is preferably 1 to 5 times, more preferably 3 to 4 times repeated extraction is not limited thereto.
상기 방법에 있어서, 단계 3)의 감압농축은 진공감압농축기 또는 진공회전증발기를 이용하는 것이 바람직하나 이에 한정하지 않는다. 또한, 건조는 감압건조, 진공건조, 비등건조, 분무건조 또는 동결건조하는 것이 바람직하나 이에 한정하지 않는다.In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but it is not limited thereto. In addition, the drying is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.
또한, 상기 알디시아 팅크토리아 추출물의 분획물은 다음과 같이 제조되는 것이 바람직하나 이에 한정하지 않는다.In addition, the fraction of the aldicia tincture extract is preferably prepared as follows, but is not limited thereto.
1) 알디시아 팅크토리아 추출물을 물에 현탁한 후, n-헥산을 가하여 n-헥산층을 분리하는 단계;1) suspending the aldicia tincture extract in water, and then separating n-hexane layer by adding n-hexane;
2) 단계 1)의 남은 물층에 클로로포름을 가하여 클로로포름 층을 분리하는 단계;2) separating the chloroform layer by adding chloroform to the remaining water layer of step 1);
3) 단계 2)의 남은 물층에 에틸아세테이트를 가하여 에틸아세테이트 층을 분리하는 단계; 3) adding ethyl acetate to the remaining water layer of step 2) to separate the ethyl acetate layer;
4) 단계 3)의 남은 물층에 부탄올을 가하여 부탄올 층을 분리하는 단계; 및4) separating butanol layer by adding butanol to the remaining water layer of step 3); And
5) 단계 4)의 남은 물층에 물 분획물을 분리하는 단계.5) separating the water fraction in the remaining water layer of step 4).
상기 방법에 있어서, 상기 유기용매는 노르말-헥산, 클로로포름, 에틸아세테이트 또는 노르말-부탄올인 것이 바람직하나 이에 한정하지 않는다. 상기 분획물은 상기 알디시아 팅크토리아 추출물로부터 분획 과정을 1 내지 5회, 바람직하게는 3회 반복하여 수득할 수 있고, 분획 후 감압 농축하는 것이 바람직하나 이에 한정하지 않는다.In the above method, the organic solvent is preferably, but not limited to, normal-hexane, chloroform, ethyl acetate, or normal-butanol. The fraction may be obtained by repeating the
상기 유기용매는 에틸아세테이트 또는 부탄올인 것이 보다 바람직하다.The organic solvent is more preferably ethyl acetate or butanol.
본 발명의 구체적인 실시예에서, 알디시아 팅크토리아 추출물의 항산화 효과를 확인하기 위하여, DPPH 자유라디칼 소거 활성을 1,1-디페닐-2-피클릴-히드라질(1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) 방법으로 측정한 결과, 알디시아 팅크토리아 추출물이 농도의존적으로 DPPH 자유라디칼의 소거 활성을 나타내는 것을 확인하였다(도 4 참조).In a specific embodiment of the present invention, in order to confirm the antioxidant effect of the aldicia tincture extract, DPPH free radical scavenging activity was determined by 1,1-diphenyl-2-piclyl-hydrazil (1,1-diphenyl-2- As measured by picryl-hydrazyl, DPPH, Sigma) method, it was confirmed that the aldicia tincture extract showed the scavenging activity of DPPH free radicals in a concentration-dependent manner (see FIG. 4).
또한, 본 발명의 또 다른 구체적인 실시예에서, 알디시아 팅크토리아 추출물의 항산화 효과를 확인하기 위하여, 알디시아 팅크토리아 추출물의 다양한 농도에 따른 ABTS 자유라디칼 소거 활성을 측정한 결과, 알디시아 팅크토리아 추출물이 농도의존적으로 ABTS 자유라디칼의 소거 활성을 나타내는 것을 확인하였다(도 5 참조).In addition, in another specific embodiment of the present invention, in order to confirm the antioxidant effect of the aldicia tincture extract, by measuring the ABTS free radical scavenging activity according to various concentrations of the aldicia tincture extract, the aldicia tincture extract It was confirmed that this concentration-dependent scavenging activity of the ABTS free radicals (see Fig. 5).
또한, 본 발명의 또 다른 구체적인 실시예에서, 알디시아 팅크토리아 추출물 또는 이의 분획물의 TLC 조사 및 UPLC 조사를 수행하였다(도 2 및 도 3 참조).Further, in another specific embodiment of the present invention, TLC irradiation and UPLC irradiation of the Aldicia tincture extract or fractions thereof were performed (see FIGS. 2 and 3).
또한, 본 발명의 또 다른 구체적인 실시예에서, 알디시아 팅크토리아 분획물의 DPPH 및 ABTS 자유라디칼 소거능을 확인한 결과, 알디시아 팅크토리아 분획물은 DPPH 및 ABTS 자유라디칼 소거능이 우수한 것을 확인하였으며, 특히 알디시아 팅크토리아 에틸아세테이트 및 부탄올 분획물이 DPPH 자유라디칼 소거 효과가 현저히 높고, ABTS 자유라디칼의 경우 전 분획물에 걸쳐 소거 효과가 현저히 우수한 것을 확인하였다(표 1 참조). In addition, in another specific embodiment of the present invention, as a result of confirming the DPPH and ABTS free radical scavenging ability of the aldicia tincture composition, it was confirmed that the aldicia tincture composition has excellent DPPH and ABTS free radical scavenging ability, in particular, the aldicia tincture Toria ethyl acetate and butanol fractions were found to have a significantly higher DPPH free radical scavenging effect, and ABTS free radicals were significantly superior to all the fractions (see Table 1).
따라서, 본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물은 현저한 DPPH 자유라디칼 소거 활성 및 ABTS 자유라디칼 소거 활성을 나타내어 탁월한 항산화 효과를 가지므로 노화방지용 화장료 조성물 또는 항산화용 화장료 조성물의 유효성분으로 유용하게 사용될 수 있음을 알 수 있다.
Therefore, the aldicia tincture extract or fractions thereof of the present invention exhibits significant DPPH free radical scavenging activity and ABTS free radical scavenging activity and thus has an excellent antioxidant effect, and thus may be usefully used as an active ingredient of an anti-aging cosmetic composition or an antioxidant cosmetic composition. It can be seen that.
상기 화장료 조성물은, 예를 들면 용액, 겔, 고체 또는 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼, 현탁액, 마이크로에멀젼, 마이크로캡슐, 미세과립구 또는 이온형(리포좀), 비이온형의 소낭 분산제의 형태, 크림, 스킨, 로션, 파우더, 연고, 스프레이 또는 콘실 스틱의 형태로 제공될 수 있다. 또한, 포말의 형태 또는 압축된 추진제를 더 함유한 에어로졸 조성물의 형태로도 제조될 수 있다.The cosmetic composition may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) A cream, a skin, a lotion, a powder, an ointment, a spray, or a cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
또한, 상기 화장료 조성물은 본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물에 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다.In addition, the cosmetic composition is in addition to the aldicia tincture extract or fractions thereof of the present invention, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents (foaming agents) , Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles Or any other ingredient commonly used in cosmetics, such as auxiliaries commonly used in the cosmetic field.
본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물을 함유하는 화장료 조성물에 있어서, 통상적으로 함유되는 화장료 조성물에 본 발명의 추출물이 0.1 내지 50 중량%, 바람직하게는 1 내지 10 중량%의 양으로 첨가될 수 있다.In the cosmetic composition containing the aldicia tincture extract of the present invention or a fraction thereof, the extract of the present invention is added to the cosmetic composition usually contained in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight. Can be.
본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물을 피부 외용제로 사용하는 경우, 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온 봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 피부용 외용제에 통상적으로 사용되는 임의의 다른 성분과 같은 피부 과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 또한, 상기 성분들은 피부 과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다.
When the aldicia tincture extract or fractions thereof of the present invention is used as an external preparation for skin, it is further added to fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents (foaming agents). ), Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipids It may contain adjuvants commonly used in the field of dermatology, such as vesicles or any other ingredients conventionally used in external preparations for skin. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.
또한, 본 발명은 알디시아 팅크토리아 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 약학적 조성물을 제공한다.The present invention also provides an anti-aging pharmaceutical composition containing an Aldicia tincture extract or a fraction thereof as an active ingredient.
상기 노화는 피부노화인 것이 바람직하나 이에 한정하지 않는다. The aging is preferably skin aging but is not limited thereto.
또한, 본 발명은 알디시아 팅크토리아 추출물 또는 이의 분획물을 유효성분으로 함유하는 항산화용 약학적 조성물을 제공한다.The present invention also provides a pharmaceutical composition for antioxidant containing Aldicia tincture extract or fractions thereof as an active ingredient.
본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물은 현저한 DPPH 자유라디칼 소거 활성 및 ABTS 자유라디칼 소거 활성을 나타내어 탁월한 항산화 효과를 가지므로 노화방지용 약학적 조성물 또는 항산화용 약학적 조성물의 유효성분으로 유용하게 사용될 수 있음을 알 수 있다.
The aldicia tincture extract or fractions thereof of the present invention exhibits significant DPPH free radical scavenging activity and ABTS free radical scavenging activity and thus has an excellent antioxidant effect, and thus can be usefully used as an active ingredient of an anti-aging pharmaceutical composition or an anti-aging pharmaceutical composition. It can be seen that.
본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물을 함유하는 조성물은 상기 성분에 추가로 동일 또는 유사한 기능을 나타내는 유효성분을 1종 이상 함유할 수 있다. The composition containing the aldicia tincturea extract of the present invention or a fraction thereof may further contain one or more active ingredients which exhibit the same or similar functions in addition to the above components.
본 발명의 조성물은 약제학적으로 허용 가능한 첨가제를 더 포함할 수 있으며, 이때 약제학적으로 허용 가능한 첨가제로는 전분, 젤라틴화 전분, 미결정셀룰로오스, 유당, 포비돈, 콜로이달실리콘디옥사이드, 인산수소칼슘, 락토스, 만니톨, 엿, 아라비아고무, 전호화전분, 옥수수전분, 분말셀룰로오스, 히드록시프로필셀룰로오스, 오파드라이, 전분글리콜산나트륨, 카르나우바 납, 합성규산알루미늄, 스테아린산, 스테아린산마그네슘, 스테아린산알루미늄, 스테아린산칼슘, 백당, 덱스트로스, 소르비톨 및 탈크 등이 사용될 수 있다. 본 발명에 따른 약제학적으로 허용 가능한 첨가제는 상기 조성물에 대해 0.1 ~ 90 중량부 포함되는 것이 바람직하나 이에 한정되는 것은 아니다.The composition of the present invention may further comprise a pharmaceutically acceptable additive, wherein the pharmaceutically acceptable additive may include starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, calcium hydrogen phosphate, lactose , Mannitol, malt, gum arabic, pregelatinized starch, corn starch, powdered cellulose, hydroxypropyl cellulose, opiodry, sodium starch glycolate, lead carnauba, synthetic aluminum silicate, stearic acid, magnesium stearate, aluminum stearate, calcium stearate , Sucrose, dextrose, sorbitol, talc and the like can be used. The pharmaceutically acceptable additive according to the present invention is preferably included 0.1 to 90 parts by weight based on the composition, but is not limited thereto.
즉, 본 발명의 조성물은 실제 임상 투여 시에 경구 및 비경구의 여러 가지 제형으로 투여될 수 있는데, 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 알디시아 팅크토리아 추출물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트(Calcium carbonate), 수크로스(Sucrose), 락토오스(Lactose) 또는 젤라틴 등을 섞어 조제될 수 있다. 또한 단순한 부형제 이외에 마그네슘 스티레이트 탈크 같은 윤활제들도 사용될 수 있다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제 및 시럽제 등이 해당되는데 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구 투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조제제, 좌제가 포함될 수 있다. 비수성용제, 현탁용제로는 프로필렌글리콜(Propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.That is, the composition of the present invention can be administered in various oral and parenteral formulations during actual clinical administration, and when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used It can be prepared using. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, water Can be prepared by mixing cross (Lucose), lactose (Lactose) or gelatin. In addition to simple excipients, lubricants such as magnesium styrate talc may also be used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents, water and liquid paraffin. . Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.
본 발명의 조성물은 목적하는 방법에 따라 경구 투여하거나 비경구 투여할 수 있으며, 비경구 투여시 피부 외용 또는 복강내주사, 직장내주사, 피하주사, 정맥주사, 근육내 주사 또는 흉부내 주사 주입방식을 선택하는 것이 바람직하다. 투여량은 환자의 체중, 연령, 성별, 건강상태, 식이, 투여시간, 투여방법, 배설율 및 질환의 중증도 등에 따라 그 범위가 다양하다.The composition of the present invention may be administered orally or parenterally according to a desired method, and when administered parenterally, external skin or intraperitoneal injection, rectal injection, subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection injection method It is preferable to select. Dosage ranges depending on the patient's weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion and the severity of the disease.
본 발명의 조성물의 투여량은 환자의 체중, 연령, 성별, 건강상태, 식이, 투여시간, 투여방법, 배설율 및 질환의 중증도에 따라 그 범위가 다양하며, 일일 투여량은 알디시아 팅크토리아 추출물의 양을 기준으로 0.0001 내지 100 ㎎/㎏이고, 바람직하게는 0.001 내지 10 ㎎/㎏이며, 하루 1 ~ 6 회 투여될 수 있다.The dosage of the composition of the present invention varies depending on the weight, age, sex, health status, diet, time of administration, administration method, excretion rate and severity of the disease of the patient, the daily dosage is the Aldicia tinctureria extract It is 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, based on the amount of can be administered 1 to 6 times a day.
본 발명의 조성물은 단독으로, 또는 수술, 방사선 치료, 호르몬 치료, 화학 치료 및 생물학적 반응 조절제를 사용하는 방법들과 병용하여 사용할 수 있다.
The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.
또한, 본 발명은 알디시아 팅크토리아 추출물 또는 이의 분획물을 유효성분으로 함유하는 노화방지용 건강식품용 조성물을 제공한다.The present invention also provides an anti-aging health food composition containing an Aldicia tincture extract or a fraction thereof as an active ingredient.
상기 노화는 피부노화인 것이 바람직하나 이에 한정하지 않는다.The aging is preferably skin aging but is not limited thereto.
또한, 본 발명은 알디시아 팅크토리아 추출물 또는 이의 분획물을 유효성분으로 함유하는 항산화용 건강식품용 조성물을 제공한다.The present invention also provides a composition for antioxidant health foods containing Aldicia tincture extract or fractions thereof as an active ingredient.
본 발명의 알디시아 팅크토리아 추출물 또는 이의 분획물은 현저한 DPPH 자유라디칼 소거 활성 및 ABTS 자유라디칼 소거 활성을 나타내어 탁월한 항산화 효과를 가지므로 노화방지용 건강식품 또는 항산화용 건강식품의 유효성분으로 유용하게 사용될 수 있음을 알 수 있다.
Aldicia tincture extract or fractions thereof of the present invention exhibits significant DPPH free radical scavenging activity and ABTS free radical scavenging activity and thus has an excellent antioxidant effect, and thus can be usefully used as an active ingredient for anti-aging health foods or antioxidant health foods. It can be seen.
본 발명의 건강식품은 알디시아 팅크토리아 추출물 또는 이의 분획물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다.The health food of the present invention may be added to the Aldicia tincture extract or fractions thereof as it is, or used with other foods or food ingredients, and may be appropriately used according to a conventional method.
상기 건강식품의 종류에는 특별한 제한은 없다. 상기 알디시아 팅크토리아 추출물 또는 이의 분획물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the kind of the health food. Examples of foods to which the Aldisia tincture extract or fractions thereof may be added include meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, other noodles, gum products, dairy products including ice cream, various soups , Beverages, tea, drinks, alcoholic beverages and vitamin complexes, etc., and includes all of the health food in the usual sense.
본 발명의 건강음료 조성물은 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖당 일반적으로 약 0.01 ~ 0.04 g, 바람직하게는 약 0.02 ~ 0.03 g 이다.The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.
상기 외에 본 발명의 건강식품은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 천연 과일주스, 과일주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 혼합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하진 않지만 본 발명의 조성물 100 중량부당 0.01 ~ 0.1 중량부의 범위에서 선택되는 것이 일반적이다.
In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. Others may contain pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
또한, 본 발명은 약학적으로 유효한 양의 알디시아 팅크토리아 추출물 또는 이의 분획물을 유효성분으로 함유하는 약학적 조성물을 개체에 투여하는 단계를 포함하는 노화억제 방법을 제공한다.The present invention also provides a method for inhibiting aging comprising administering to a subject a pharmaceutical composition containing a pharmaceutically effective amount of an Aldicia tincture extract or a fraction thereof as an active ingredient.
상기 노화는 피부노화인 것이 바람직하나 이에 한정하지 않는다.The aging is preferably skin aging but is not limited thereto.
상기 약학적으로 유효한 양이란 0.00001 내지 10 mg/kg이고, 바람직하게는 0.0001 내지 1 mg/kg이나 이에 한정하지 않는다. 투여량은 특정 환자의 체중, 연령, 성별, 건강상태, 식이, 투여기간, 투여방법, 제거율, 질환의 중증도 등에 따라 변화될 수 있다. The pharmaceutically effective amount is 0.00001 to 10 mg / kg, preferably 0.0001 to 1 mg / kg, but is not limited thereto. The dose may vary depending on the weight, age, sex, health condition, diet, administration period, method of administration, rate of elimination, severity of disease, and the like of a particular patient.
상기 개체는 척추동물이고 바람직하게는 포유동물이며, 그보다 바람직하게는 쥐, 토끼, 기니아피크, 햄스터, 개, 고양이와 같은 실험동물이고, 가장 바람직하게는 침팬지, 고릴라와 같은 유인원류 동물이다. The subject is a vertebrate and preferably a mammal, more preferably an experimental animal such as a rat, rabbit, guinea pig, hamster, dog, cat, and most preferably an ape-like animal such as a chimpanzee or gorilla.
상기 투여 방법은 경구 또는 비경구가 바람직하며, 피부 도포가 가장 바람직하다.
The administration method is preferably oral or parenteral, most preferably skin application.
이하, 본 발명을 실시예, 실험예 및 제조예에 의하여 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Production Examples.
단, 하기 실시예, 실험예 및 제조예는 본 발명을 구체적으로 예시하는 것일 뿐, 본 발명의 내용이 실시예, 실험예 및 제조예에 의해 한정되는 것은 아니다.
However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention in detail, and the content of the present invention is not limited to Examples, Experimental Examples, and Preparation Examples.
<< 실시예Example 1> 1> 알디시아Aldicia 팅크토리아Tincture 추출물의 제조 Preparation of extract
본 발명의 알디시아 팅크토리아 추출물은 한국생명공학연구원 해외생물소재허브센터의 해외식물추출물은행에서 구입하였으며 한국생명공학연구원 식물표본관(KRIB, Herbarium of the Korea Research Institute of Bioscience and Biotechnology)에 증거표본(KRIB 0027026)이 보관되어 있다. 추출과정을 살펴보면, 알디시아 팅크토리아(잎, 어린가지, 꽃)를 건조한 후, 분쇄된 분말시료 113 g을 메탄올 1 ℓ를 가한 후 초음파추출기(SDN-900H, SD-ULTRASONIC CO., LTD)를 이용하여 상온에서 30회(40KHz, 15분 추출-120분 정지) 반복추출하였다. 그런 다음 여과하여 감압농축한 후, 알디시아 팅크토리아 메탄올 추출물(이하 “알디시아 팅크토리아 추출물”이라 함) 10.33 g을 수득하였다.
The Aldicia tincture extract of the present invention was purchased from the Overseas Plant Extract Bank of the Korea Center for Biomaterials and Herbal Development, Korea Research Institute of Bioscience and Biotechnology, and the specimens were collected in the Herbarium of the Korea Research Institute of Bioscience and Biotechnology (KRIB). KRIB 0027026). In the extraction process, after drying Aldicia tincture (leaves, sprigs and flowers), 113 g of pulverized powder sample was added to 1 L of methanol, followed by ultrasonic extractor (SDN-900H, SD-ULTRASONIC CO., LTD). 30 times (40KHz, 15 minutes extraction-120 minutes stop) was repeatedly extracted at room temperature. After filtration and concentration under reduced pressure, 10.33 g of aldicia tincture methanol extract (hereinafter referred to as “aldicia tincture extract”) were obtained.
<< 실시예Example 2> 2> 알디시아Aldicia 팅크토리아의Tincture 용매 menstruum 분획물Fraction 제조 Produce
상기 <실시예 1>에서 얻어진 알디시아 팅크토리아 추출물 중 450 mg 에 증류수를 첨가하여 현탁시키고, 동량의 n-헥산을 가하여 혼합 후 n-헥산가용성 분획부와 물가용성 분획부를 분리하였으며, 이를 3회 실시하여 여과, 감압농축하여 n-헥산 분획물 90.4 mg을 수득하였다. 그런 다음, 상기 n-헥산 분획물을 제거하고 남은 물층에 클로로포름을 동량 가하여 같은 방법으로 클로로포름 분획물 22.0 mg 수득하였고, 다시 남은 물층에 에틸아세테이트를 동량 가하여 같은 방법으로 에틸아세테이트 분획물 44.8 mg을 수득하였으며, 또다시 남은 물층에 부탄올을 동량 가하여 동일한 방법으로 부탄올 분획물 57.6 mg을 수득하였으며, 남은 물층을 농축하여 물 분획물 205.2 mg을 수득하였다(도 1).
Suspended by adding distilled water to 450 mg of the aldicia tincture extract obtained in <Example 1>, and mixed with an equal amount of n-hexane, the n-hexane-soluble fraction and the water-soluble fraction were separated, and this was repeated three times. Filtration and concentration under reduced pressure gave 90.4 mg of n-hexane fractions. Then, by removing the n-hexane fraction and adding the same amount of chloroform to the remaining water layer to give 22.0 mg of the chloroform fraction in the same manner, to the remaining water layer was added to the same amount of ethyl acetate to obtain 44.8 mg of ethyl acetate fraction in the same manner. The same amount of butanol was added to the remaining water layer to give 57.6 mg of butanol fraction in the same manner, and the remaining water layer was concentrated to obtain 205.2 mg of water fraction (FIG. 1).
<< 실시예Example 3> 3> 알디시아Aldicia 팅크토리아Tincture 추출물 또는 이의 Extract or its 분획물의Fraction TLCTLC 조사 Research
상기 <실시예 1>과 <실시예 2>에서 얻어진 알디시아 팅크토리아 추출물과 분획물을 10 mg/ml의 농도로 메탄올에 녹인 후 실리카겔 박층 크로마토그래피(TLC Silica gel 60 F254, Merck)를 시행하여 비극성물질들을 확인하였다. 전개용매로는 n-헥산: 아세톤의 비율이 2 : 1인 혼합용매로 하였다. 또한, 극성물질들을 확인해 보기 위해 실리카겔 역상 크로마토그래피(TLC Silica gel 60 RP-18 F254S, Merck)를 실시하였고, 전개용매는 메탄올 50%로 하였다(도 2).
The Aldicia tincture extract and fractions obtained in <Example 1> and <Example 2> were dissolved in methanol at a concentration of 10 mg / ml, and then subjected to silica gel thin layer chromatography (TLC Silica gel 60 F 254 , Merck). Nonpolar materials were identified. As a developing solvent, it was set as the mixed solvent whose ratio of n-hexane: acetone is 2: 1. In addition, to confirm the polar substances, silica gel reversed phase chromatography (
<< 실시예Example 4> 4> 알디시아Aldicia 팅크토리아의Tincture 추출물 또는 이의 Extract or its 분획물의Fraction UPLCUPLC 분석 analysis
상기 <실시예 1>과 <실시예 2>에서 얻어진 알디시아 팅크토리아 추출물과 분획물을 10mg/ml로 메탄올에 녹인 후 0.2 um의 membrane filter로 여과하였다. UPLC는 Waters Acquity UPLC 시스템을 이용하였으며, 컬럼은 ACQUITY UPLCTMBEHC18(10mmⅩ 2.1 mm, i.d., 1.7μm, waters, USA)을 35℃에서 사용하였으며, 이동상 A는 물과 formic acid (100:0.1, v/v)로 이동상 B는 acetonitril과 formic acid (100:0.1,v/v)로 하여 초기에 이동상 B를 10%로 1분 동안 유지시킨 후, 5분까지 45%로 증가, 7.5분까지 100%로 이동상 B를 증가시켜 분석한 후, 이동상 B를 100%로 9분까지 유지시키고, 9.5분까지 이동상 B를 10%로 낮추고 11분까지 10%로 안정화시켜 분석에 사용하였다. 이동상의 유속량은 0.4 ㎖/min 그리고 시료 주입량은 3 ㎕로 하였다. The aldicia tincture extract and fractions obtained in <Example 1> and <Example 2> were dissolved in methanol at 10 mg / ml and filtered through a 0.2 um membrane filter. UPLC was used for the Waters Acquity UPLC system, column was ACQUITY UPLC TM BEHC 18 (10mmⅩ 2.1 mm, id, 1.7μm, waters, USA) at 35 ℃, mobile phase A was water and formic acid (100: 0.1, v / v), mobile phase B is acetonitril and formic acid (100: 0.1, v / v) and initially maintains mobile phase B at 10% for 1 minute, then increases to 45% up to 5 minutes, 100 up to 7.5 minutes. After analysis by increasing mobile phase B by%, the mobile phase B was maintained at 100% for 9 minutes, the mobile phase B was lowered to 10% by 9.5 minutes and stabilized at 10% by 11 minutes for use in the analysis. The flow rate of the mobile phase was 0.4 ml / min and the sample injection amount was 3 µl.
CAD(Charge Aerosol Detector)는 UPLC로부터 분리되어진 물질들에 전하(Charge)를 붙여주어 그 전하를 측정하는 detector로써 크로마토그래피 형식으로 물질의 상대적 함량과 분리도를 확인해 볼 수 있다 (도 3). CAD (Charge Aerosol Detector) is a detector that measures the charge by attaching a charge (Charge) to the material separated from the UPLC can check the relative content and the degree of separation in the chromatographic form (Fig. 3).
<< 실험예Experimental Example 1> 1> 알디시아Aldicia 팅크토리아Tincture 추출물의 Extract DPPHDPPH 자유라디칼Free radical 소거 활성 확인 Confirm erase activity
상기 <실시예 1>의 알디시아 팅크토리아 추출물에 대하여 자유라디칼 소거 활성을 1,1-디페닐-2-피크릴-히드라질(1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) 방법으로 측정하였다. The free radical scavenging activity of 1,1-diphenyl-2-picryl-hydrazyl (1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) with respect to the aldicia tincture extract of <Example 1> It was measured by the method.
구체적으로, 자유라디칼 소거 활성 측정에는 에탄올에 잘 녹인 0.15 mM의 DPPH 용액 90 ㎕을 96 웰 플레이트에 넣고, 상기 추출물을 2.5, 5, 10, 20, 40 및 80 ㎍/㎖의 농도로 각각 분주하여, 상온에서 20분간 방치한 후, ELISA 리더(reader)로 517 nm에서 흡광도를 측정하였다. 자유라디칼 소거 활성(%)은 하기의 수학식 1로 산출하였다. 이때, DMSO를 처리한 군을 음성대조군으로, 합성 항산화제인 BHA와 천연항산화제인 토코페롤을 처리한 군을 양성대조군으로 사용하였다.
Specifically, to measure the free radical scavenging activity, 90 μl of a 0.15 mM DPPH solution dissolved in ethanol was put in a 96 well plate, and the extracts were dispensed at concentrations of 2.5, 5, 10, 20, 40, and 80 μg / ml, respectively. After 20 minutes at room temperature, the absorbance was measured at 517 nm with an ELISA reader. Free radical scavenging activity (%) was calculated by the following equation (1). At this time, the DMSO-treated group was used as a negative control group, and the group treated with the synthetic antioxidant BHA and the natural antioxidant tocopherol was used as a positive control group.
A: 본 발명의 알디시아 팅크토리아 추출물을 처리하지 않은 음성대조군의 흡광도; 및A: absorbance of the negative control group not treated with the aldicia tincture extract of the present invention; And
B: 본 발명의 알디시아 팅크토리아 추출물을 처리한 실험군의 흡광도.
B: Absorbance of the experimental group treated with the aldicia tincture extract of the present invention.
그 결과, 도 4에 나타낸 바와 같이, 알디시아 팅크토리아 추출물에 대한 DPPH 자유라디칼 소거 활성을 측정한 결과, 알디시아 팅크토리아 추출물의 처리 농도에 의존적으로 DPPH 자유라디칼 소거 활성을 나타냄을 확인하였고, 양성대조군인 BHA와 토크페롤과 비교하여 우수한 DPPH 자유라디칼 소거활성을 나타내는 것을 확인하였다(도 4).
As a result, as shown in FIG. 4, DPPH free radical scavenging activity of the aldicia tincture extract was measured. As a result, it was confirmed that DPPH free radical scavenging activity was shown depending on the treatment concentration of the aldicia tincture extract. Compared with the control group BHA and tocopherol it was confirmed that the excellent DPPH free radical scavenging activity (Fig. 4).
<< 실험예Experimental Example 2> 2> 알디시아Aldicia 팅크토리아Tincture 추출물의 Extract ABTSABTS 자유라디칼Free radical 소거 활성 확인 Confirm erase activity
상기 <실시예 1>의 알디시아 팅크토리아 추출물을 이용하여 ABTS 자유라디칼 소거 활성을 확인하였다. ABTS free radical scavenging activity was confirmed using the aldicia tincture extract of <Example 1>.
구체적으로, ABTS 자유라디칼 소거 활성은 ABTS와 포타슘 퍼설페이트(potassium persulfate)를 각각 7 mM과 2.45 mM의 최종농도가 되도록 혼합한 후, 암소에서 12시간 방치하여 ABTS 라디칼을 형성시켰다. 이를 734 nm에서 흡광도가 0.7이 되도록 증류수로 희석한 후 96 웰 플레이트에 분주하고, 여기에 시료를 2.5, 5, 10, 20, 40 및 80 ㎍/㎖의 농도로 분주한 후, 상온의 암소에서 7분간 반응시켰다. 흡광도는 734 nm에서 마이크로플레이트 측정기를 이용하여 측정하였다. 이때, DMSO를 처리한 군을 음성대조군으로, 합성 항산화제인 BHA와 토크페놀을 처리한 군을 양성대조군으로 사용하였다.Specifically, ABTS free radical scavenging activity was mixed with ABTS and potassium persulfate to a final concentration of 7 mM and 2.45 mM, respectively, and left in the dark for 12 hours to form ABTS radicals. It was diluted with distilled water so as to have an absorbance of 0.7 at 734 nm and then aliquoted into 96 well plates, and the samples were dispensed at concentrations of 2.5, 5, 10, 20, 40 and 80 μg / ml, followed by The reaction was carried out for 7 minutes. Absorbance was measured using a microplate meter at 734 nm. At this time, the group treated with DMSO was used as a negative control group, and the group treated with synthetic antioxidants BHA and torque phenol was used as a positive control group.
그 결과, 도 5에서 나타낸 바와 같이 알디시아 팅크토리아 추출물에 대한 ABTS 자유라디칼 소거 활성을 측정한 결과, 알디시아 팅크토리아 추출물의 처리 농도에 의존적으로 ABTS 자유라디칼 소거 활성을 나타냄을 확인하였고, 양성대조군인 BHA와 토크페롤과 비교하여 유사한 ABTS 라디칼 소거활성을 나타내는 것을 확인하였다(도 5).
As a result, as shown in FIG. 5, the ABTS free radical scavenging activity of the aldicia tincture extract was measured. As a result, it was confirmed that the ABTS free radical scavenging activity was shown depending on the treatment concentration of the aldicia tincture extract. It was confirmed that similar ABTS radical scavenging activity compared to phosphorus BHA and tocopherol (FIG. 5).
<< 실험예Experimental Example 3> 3> 알디시아Aldicia 팅크토리아Tincture 용매 menstruum 분획물의Fraction DPPHDPPH 및 And ABTSABTS 자유라디칼Free radical 소거능Scatters
상기 <실시예 2>에서 분획한 용매별 n-헥산, 클로로포름, 에틸아세테이트, 부탄올, 물 분획물에 대해 <실험예 1> 및 <실험예 2>와 동일한 방법으로 DPPH 및 ABTS 자유라디칼 소거능을 측정하였다. DPPH and ABTS free radical scavenging ability of n-hexane, chloroform, ethyl acetate, butanol, and water fractions of each solvent fractionated in <Example 2> were measured in the same manner as in <Experimental Example 1> and <Experimental Example 2>. .
구체적으로, 상기 분획한 각 분획물은 40 ㎍/㎖의 농도로 각각 분주하여, 상온에서 반응시킨 후, 마이크로플레이트 흡광기로 흡광도를 측정하였다. 이때, DMSO를 처리한 군을 음성대조군으로, 천연항산화제인 a-토코페롤과 아스코르브산을 처리한 군과 합성항산화제인 BHA를 처리한 군을 양성대조군으로 사용하였다. Specifically, each fraction was fractionated at a concentration of 40 ㎍ / ㎖, respectively, and reacted at room temperature, the absorbance was measured by a microplate absorber. At this time, the group treated with DMSO was used as a negative control group, the group treated with natural antioxidants a-tocopherol and ascorbic acid and the group treated with synthetic antioxidant BHA were used as positive controls.
그 결과, 표 1에서 나타난 바와 같이, 알디시아 팅크토리아 용매 분획물은 DPPH 및 ABTS 자유라디칼 소거 효과가 뛰어남을 확인하였고, 특히 알디시아 팅크토리아 에틸아세테이트, 부탄올 분획물은 DPPH 자유라디칼 소거 효과가 현저히 높고 ABTS 자유라디칼의 경우 전 분획물에 걸쳐 소거 효과가 우수함을 확인하였다. 또한, 천연물 유래의 양성대조군인 a-토코페롤과 아스코르브산을 처리한 군과 비교하였을 때도 월등히 우수한 활성을 나타냄을 확인하였다(표 1).As a result, as shown in Table 1, it was confirmed that the Aldicia tintoria solvent fractions were excellent in DPPH and ABTS free radical scavenging effects. In particular, the Aldisia tintoria ethyl acetate and butanol fractions were significantly higher in DPPH free radical scavenging effects and ABTS. In the case of free radicals, it was confirmed that the scavenging effect was excellent over all fractions. In addition, it was confirmed that when compared with the group treated with a-tocopherol and ascorbic acid, which is a positive control group derived from natural products, it was confirmed to show excellent activity (Table 1).
<< 제조예Manufacturing example 1> 1> 알디시아Aldicia 팅크토리아Tincture 추출물을 유효성분으로 함유하는 노화방지용 Anti-aging containing extract as an active ingredient 화장료의Cosmetic 제조 Produce
<1-1> 유연 화장수의 제조<1-1> Manufacture of flexible lotion
상기 <실시예 1>의 알디시아 팅크토리아 추출물을 유효성분으로 함유하는 유연 화장수는 하기 [표 2]와 같이 제조하였다.
The flexible lotion containing the aldicia tincture extract of <Example 1> as an active ingredient was prepared as shown in [Table 2].
<1-2> 영양 크림의 제조<1-2> Preparation of nourishing cream
상기 <실시예 2>의 알디시아 팅크토리아 추출물을 유효성분으로 함유한 영양크림은 하기 [표 3]의 조성과 같이 제조하였다.
The nutrition cream containing the Aldicia tincture extract of <Example 2> as an active ingredient was prepared as in the composition of the following [Table 3].
<< 제조예Manufacturing example 2> 2> 알디시아Aldicia 팅크토리아Tincture 추출물을 유효성분으로 함유하는 약학적 제제의 제조 Preparation of a pharmaceutical formulation containing the extract as an active ingredient
<2-1> <2-1> 산제의Sanje 제조 Produce
본 발명의 알디시아 팅크토리아 추출물 2 gAldicia tincture extract 2g of the present invention
유당 1 g1 g lactose
상기의 성분을 혼합하고 기밀포에 충진하여 산제를 제조하였다.
The above components were mixed and packed in airtight bags to prepare powders.
<2-2> 정제의 제조<2-2> Preparation of Tablet
본 발명의 알디시아 팅크토리아 추출물 100 ㎎
옥수수전분 100 ㎎
유 당 100 ㎎
스테아린산 마그네 2 ㎎
상기의 성분을 혼합한 후, 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.
After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.
<2-3> 캡슐제의 제조<2-3> Preparation of Capsule
본 발명의 알디시아 팅크토리아 추출물 100 ㎎
옥수수전분 100 ㎎
유 당 100 ㎎
스테아린산 마그네슘 2 ㎎2 mg magnesium stearate
상기의 성분을 혼합한 후, 통상의 캡슐제의 제조방법에 따라서 젤라틴 캡슐에 충전하여 캡슐제를 제조하였다.
After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.
<2-4> 환의 제조<2-4> Preparation of the ring
본 발명의 알디시아 팅크토리아 추출물 1 gAldicia tincture extract 1g of the present invention
유당 1.5 gLactose 1.5 g
글리세린 1 g1 g of glycerin
자일리톨 0.5 gXylitol 0.5 g
상기의 성분을 혼합한 후, 통상의 방법에 따라 1환 당 4 g이 되도록 제조하였다.
After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.
<2-5> 과립의 제조<2-5> Preparation of Granules
본 발명의 알디시아 팅크토리아 추출물 150 ㎎Aldicia tincture extract 150mg of the present invention
대두추출물 50 ㎎Soy extract 50 mg
포도당 200 ㎎
전분 600 ㎎
상기의 성분을 혼합한 후, 30% 에탄올 100 ㎎을 첨가하여 섭씨 60 ℃에서 건조하여 과립을 형성한 후 포에 충진하였다.
After mixing the above components, 100 mg of 30% ethanol was added, dried at 60 ° C. to form granules, and then filled into fabrics.
<< 제조예Manufacturing example 3> 3> 알디시아Aldicia 팅크토리아Tincture 추출물을 유효성분으로 함유하는 노화방지용 건강식품의 제조 Preparation of anti-aging health food containing extract as an active ingredient
<3-1> 밀가루 식품의 제조<3-1> Production of flour food
본 발명의 알디시아 팅크토리아 추출물 0.5~5.0 중량부를 밀가루에 첨가하고, 이 혼합물을 이용하여 빵, 케이크, 쿠키, 크래커 및 면류를 제조하였다.
0.5 to 5.0 parts by weight of the aldicia tincture extract of the present invention was added to the flour, and bread, cake, cookies, crackers and noodles were prepared using this mixture.
<3-2> <3-2> 스프soup 및 육즙( And juicy ( graviesgravies )의 제조Manufacturing
본 발명의 알디시아 팅크토리아 추출물 0.1~5.0 중량부를 스프 및 육즙에 첨가하여 건강 증진용 육가공 제품, 면류의 수프 및 육즙을 제조하였다.
0.1-5.0 parts by weight of the aldicia tincture extract of the present invention was added to soups and broths to prepare meat products for health promotion, soups of noodles and broths.
<3-3> 그라운드 <3-3> Ground 비프(ground beef)의Beef 제조 Produce
본 발명의 알디시아 팅크토리아 추출물 10 중량부를 그라운드 비프에 첨가하여 건강 증진용 그라운드 비프를 제조하였다.
10 parts by weight of the aldicia tincture extract of the present invention was added to the ground beef to prepare a ground beef for health promotion.
<3-4> 유제품(<3-4> Dairy products ( dairydairy productsproducts )의 제조Manufacturing
본 발명의 알디시아 팅크토리아 추출물 5~10 중량부를 우유에 첨가하고, 상기 우유를 이용하여 버터 및 아이스크림과 같은 다양한 유제품을 제조하였다.
5 to 10 parts by weight of the aldicia tincture extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.
<3-5> <3-5> 선식의Solar 제조 Produce
현미, 보리, 찹쌀, 율무를 공지의 방법으로 알파화시켜 건조시킨 것을 배전한 후 분쇄기로 입도 60 메쉬의 분말로 제조하였다.Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.
검정콩, 검정깨, 들깨도 공지의 방법으로 쪄서 건조시킨 것을 배전한 후 분쇄기로 입도 60 메쉬의 분말로 제조하였다.Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.
본 발명의 알디시아 팅크토리아 추출물을 진공 농축기에서 감압농축하고, 분무, 열풍건조기로 건조하여 얻은 건조물을 분쇄기로 입도 60 메쉬로 분쇄하여 건조분말을 얻었다.The aldicia tincture extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a sprayer and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.
상기에서 제조한 곡물류, 종실류 및 본 발명의 알디시아 팅크토리아 추출물을 다음의 비율로 배합하여 제조하였다:The grains, seeds and the Aldicia tincture extract of the present invention prepared above were formulated in the following proportions:
곡물류(현미 30 중량부, 율무 15 중량부, 보리 20 중량부),(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)
종실류(들깨 7 중량부, 검정콩 8 중량부, 검정깨 7 중량부),Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)
본 발명의 알디시아 팅크토리아 추출물(3 중량부),Aldicia tincture extract of the present invention (3 parts by weight),
영지(0.5 중량부),(0.5 part by weight),
지황(0.5 중량부).
Rhubarb (0.5 parts by weight).
<< 제조예Manufacturing example 4> 4> 알디시아Aldicia 팅크토리아Tincture 추출물을 유효성분으로 함유하는 노화방지용 음료의 제조 Preparation of anti-aging beverages containing extracts as active ingredients
<4-1> <4-1> 건강음료의Health drink 제조 Produce
액상과당(0.5%), 올리고당(2%), 설탕(2%), 식염(0.5%), 물(75%)과 같은 부재료와 본 발명의 알디시아 팅크토리아 추출물 5 g을 균질하게 배합하여 순간 살균을 한 후 이를 유리병, 패트병 등 소포장 용기에 포장하여 제조하였다.
Instantly mix the subsidiary ingredients such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%) with 5 g of Aldicia tincture extract. After sterilization, it was prepared by packing it in a small packaging container such as a glass bottle or a plastic bottle.
<4-2> 야채 주스의 제조<4-2> Production of vegetable juice
본 발명의 알디시아 팅크토리아 추출물 5 g을 토마토 또는 당근 주스 1,000 ㎖에 가하여 야채 주스를 제조하였다.
Vegetable juice was prepared by adding 5 g of aldicia tincture extract of the present invention to 1,000 ml of tomato or carrot juice.
<4-3> 과일 주스의 제조<4-3> Preparation of fruit juice
본 발명의 알디시아 팅크토리아 추출물 1 g을 사과 또는 포도 주스 1,000 ㎖ 에 가하여 과일 주스를 제조하였다.
Fruit juice was prepared by adding 1 g of Aldicia tincture extract of the present invention to 1,000 ml of apple or grape juice.
상기에서 보는 바와 같이, 본 발명의 알디시아 팅크토리아(Ardisia tinctoria) 추출물 및 이의 분획물은 우수한 항산화 효과를 나타내므로, 노화방지용 화장료 조성물, 약학적 조성물 및 건강식품용 조성물의 유효성분으로 유용하게 이용될 수 있다.
As seen above, the Aldicia tincture of the present invention ( Ardisia) tinctoria) extracts and fractions thereof have excellent antioxidant effects, and thus may be usefully used as an active ingredient in anti-aging cosmetic compositions, pharmaceutical compositions, and health food compositions.
Claims (9)
Ardisia tincture tinctoria ) Anti- aging cosmetic composition containing an extract or a fraction thereof as an active ingredient.
The method of claim 1, wherein the aldicia tincture extract is water, C 1 Anti-aging cosmetic composition characterized in that the extraction using a lower alcohol or a mixture of C 2 ~ as a solvent.
The anti-aging cosmetic composition according to claim 2, wherein the lower alcohol is ethanol or methanol.
The method of claim 1, wherein the fraction is a normal-hexane fraction obtained by suspending the aldicia tincture extract in water and then sequentially fractionated with normal-hexane, chloroform, ethyl acetate, normal-butanol and water. Anti-aging cosmetic composition, characterized in that any one of the acetate fraction, normal-butanol fraction or water fraction.
Anti-aging pharmaceutical composition containing Aldicia tincture extract or fractions thereof as an active ingredient.
The method of claim 5, wherein the aldicia tincture extract is water, C 1 Anti-aging pharmaceutical composition characterized in that the extraction using a lower alcohol or a mixture of C 2 ~ as a solvent.
7. The anti-aging pharmaceutical composition according to claim 6, wherein the lower alcohol is ethanol or methanol.
The method of claim 5, wherein the fraction is a normal-hexane fraction obtained by suspending the aldicia tincture extract in water and then sequentially fractionated with normal-hexane, chloroform, ethyl acetate, normal-butanol and water. Anti-aging pharmaceutical composition, characterized in that any one of the acetate fraction, normal- butanol fraction or water fraction.
Anti-aging health food composition containing Aldicia tincture extract or fractions thereof as an active ingredient.
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