KR20120140315A - Composition for preventing or treating asthma or atopy - Google Patents

Abstract

PURPOSE: A composition containing Glycyrrhizae radix and ginger complex extract is provided to effectively prevent or treat asthma and atopic dermatitis. CONSTITUTION: A composition for preventing or treating asthma or atopic dermatitis contains Glycyrrhizae radix and ginger complex extract as an active ingredient. A pharmaceutical composition for preventing or treating asthma or atopic dermatitis contains the extract as an active ingredient. The Glycyrrhizae radix and ginger are mixed in a ratio of 1:1-1:3. The pharmaceutical composition additionally contains pharmaceutically acceptable carriers, excipients, or diluents. A food or a cosmetic composition for preventing or treating asthma or atopic dermatitis contains the extract as an active ingredient. [Reference numerals] (AA) IFN-γ(%); (BB, DD) Glycyrrhizae radix and ginger(μg/ml); (CC) IL-4(%)

Description

Composition for preventing or treating asthma or atopy

The present invention relates to a composition for the treatment or prevention of asthma or atopy.

Asthma and atopy are diseases associated with overreaction of T helper (Th) 2 lymphocytes to antigens. That is, the Th 2 response among the T helper (Th) lymphocyte and the T helper (Th) lymphocyte reaction is an inflammatory disease that occurs abnormally strongly. Th 1 and Th 2 reactions are mutually inhibiting reactions. When the Th 2 reaction is strong, such as asthma and atopy, the Th 1 reaction hardly occurs. This situation is called Th 2 polarization and it is well known that it is associated with asthma and atopy (Curr Probl Dermatol, 2011; 41: 136). In addition, it has been reported that the prevention of Th 2 responses can prevent the progression of diseases such as atopy and asthma (Clin Exp Immunol, 2011; 165: 19).

The problem to be solved by the present invention is to provide a composition for the treatment or prevention of asthma and atopy.

The present inventors have made intensive efforts to develop an effective pharmaceutical composition for treating asthma or atopic dermatitis in natural products with excellent safety. Surprisingly, licorice and ginger complex extracts have been shown to be effective in increasing interferon-γ secretion and interleukin-4 secretion. The present invention was confirmed to show an unexpected effect from a single extract.

The complex extract of licorice and ginger of the present invention exhibits a prophylactic or therapeutic effect of asthma and atopy by balancing Th 1 and Th 2 responses by increasing the response of Th 1 and relatively suppressing the response of Th 2.

The present invention provides a composition for improving or preventing asthma or atopy consisting of licorice and ginger complex extract as an active ingredient.

The present invention provides a pharmaceutical composition for the prevention or treatment of asthma or atopy consisting of licorice and ginger complex extract as an active ingredient.

The present invention also provides a food composition for improving or preventing asthma or atopy consisting of a licorice and ginger complex extract as an active ingredient.

The present invention provides a cosmetic composition for improving or preventing atopy consisting of the licorice and ginger complex extract as an active ingredient.

In the present invention, the composite extract of licorice and ginger is not only an extract obtained by extracting licorice and ginger together (a mixture of licorice and ginger), but also a mixture of extracts obtained by extracting each of them alone (mixture of licorice extract and ginger extract). It shows all.

'Licorice' and 'ginger' means all or part of the licorice and ginger, preferably may be the root of licorice and ginger.

In the complex extract of licorice and ginger of the present invention, the ratio of licorice and ginger to achieve a desirable effect is about 1: 1 to about 1: 3, more preferably about 1: 1.5 to 1: 2.5, most preferably Is about 1: 2. The complex extract of licorice and ginger may have a significant effect in the above range.

The licorice and ginger complex extract water licorice and ginger, c ₁ C ₄ , alcohol, and water and C ₁ ~ From the group consisting of a mixture of the C ₄ alcohol to perform the extraction process comprising extracting the selected extraction solvent (first extraction solvent) may be obtained. The primary extraction solvent is preferably water. The complex extract of licorice and ginger may also be obtained by extracting licorice and ginger, respectively, by the above method to obtain a licorice extract and a ginger extract, and then mixing them.

The extraction process may be performed by extracting with the primary extraction solvent about 2 to 20 times, preferably about 5 to 10 times the weight of licorice and ginger. Extraction may be performed by an extraction method known in the art, such as cold soaking, hot water extraction, ultrasonic extraction, reflux cooling extraction, but is not limited thereto. The extraction temperature may be adopted by those skilled in the art in various temperature ranges suitable for the extraction method, for example, may be performed at 80 ℃ to 100 ℃, but is not limited thereto. In addition, the extraction time is different depending on the extraction method, a person skilled in the art can adopt an appropriate extraction time, but is not limited to this, it can be performed in a single or multiple times in the range of about 6 hours to 1 day.

Extract obtained by performing extraction with the primary extraction solvent is obtained in a liquid form by removing impurities by filtration in accordance with a conventional method, or concentrated liquid extract and concentrated in a reduced pressure and / or dried in accordance with a conventional method in the form of a powder You can get it.

In addition, the extraction process may further comprise the step of obtaining a fraction having a high content of the active ingredient, if necessary. That is, by dispersing the extract obtained by extraction with the primary extraction solvent in water, and then extracting with an appropriate secondary extraction solvent, for example, the alcohol of saturated C4, to further increase the active ingredient content in the resulting extract Can be.

The content of the licorice and ginger complex extract in the composition of the present invention can be appropriately adjusted according to the weight, age, sex, health condition, diet, etc. of the subject, the composition of the present invention is 0.1 to 20% by weight of the active ingredient with respect to the total weight of the composition It may contain%.

The pharmaceutical composition of the present invention may be formulated with a pharmaceutically or food-acceptable carrier, excipient or diluent, etc., in addition to the above-mentioned active ingredients. For formulation, see Remington's Pharmaceutical Science (Recent Edition), Mack Publishing. See, eg, Company, Easton PA.

In an embodiment of the present invention, the pharmaceutical composition according to the present invention can be prepared in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories or sterile injections, respectively, according to conventional methods. It may be used in the form of a solution or the like.

Carriers, excipients and diluents which may be included here include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl Cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like, but are not limited thereto.

In addition, the pharmaceutical compositions of the present invention may include diluents or excipients such as fillers, extenders, binders, humectants, disintegrating agents, surfactants, and other pharmaceutically acceptable additives which are commonly used.

When formulated into solid preparations for oral administration, tablets, pills, powders, granules, capsules and the like are possible, and such solid preparations may include at least one excipient such as starch, calcium carbonate (ginsenoside Rd) ( It can be prepared by mixing calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium styrate talc may also be used.

When formulated as a liquid preparation for oral administration, suspensions, solvents, emulsions, syrups, and the like are possible, and various excipients, for example, wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin Etc. may be included.

When formulated into preparations for parenteral administration, sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories are included. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like may be used, but are not limited thereto. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like may be used, but are not limited thereto.

When the pharmaceutical composition of the present invention is a use for the prophylaxis or treatment of atopy, as a preferred embodiment the pharmaceutical composition of the present invention may be a transdermal dosage form, specific examples are ointments, gels, creams, lotions, coating agents, It may be formulated in the form of an external solution, tincture, external acid, spray, patch and the like. Such formulations may be prepared by conventional methods in the art.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. For the preferred effect, the dosage of the composition of the present invention is 10 mg / kg to 100 mg / kg per adult male based on the complex extract content of licorice and ginger as active ingredients.

The administration may be carried out once a day or divided into several doses. However, the scope of the present invention is not limited by the dose and frequency of administration.

The pharmaceutical compositions of the present invention can be used in mammals such as rats, mice, livestock, humans, and the like, for example, orally, or intraperitoneally, rectally, intravenously, intramuscularly, subcutaneously, intrauterine dural or brain. It can be administered by injection or the like intravascularly (Intracerebroventricular) or applied locally by smearing on affected area.

The pharmaceutical compositions of the present invention can be used for the prevention or treatment of atopy or asthma. Accordingly, the present invention relates to the use of the licorice and ginger complex extract for the prevention or treatment of atopy or asthma, the use of the licorice and ginger complex extract for the preparation of a medicament for the prevention or treatment of atopy or asthma, pharmaceuticals for mammals including humans Provided are methods for the prophylaxis or treatment of atopic or asthma comprising administering a scientifically effective amount of licorice and ginger complex extract.

The present invention provides a food composition for improving or preventing asthma or atopy consisting of a licorice and ginger complex extract as an active ingredient.

The food composition of the present invention can be used as a dietary supplement. "Health functional food" means a food manufactured and processed using raw materials or ingredients with functional properties useful to humans under No. 6727 of the Health Functional Food Act. "Functional" means the structure and It means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action with respect to function.

The food composition of the present invention may include a conventional food additive in addition to the above-mentioned active ingredient, and the suitability as a "food additive" is general rule of the Food Additives Code and General approved by the Food and Drug Administration, unless otherwise specified. It is determined by the standards and standards for the relevant items according to the test method.

Examples of the items described in the "Food Additive Reduction" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamon acid, natural additives such as color pigments, licorice extract, crystalline cellulose, high color pigments and guar gum, and L. And mixed preparations such as sodium glutamate preparation, noodle addition, preservative preparation and tar coloring preparation.

The health functional food composition of the present invention can be used in a variety of foods and drinks for the prevention or improvement of atopy or asthma, for example, various foods, beverages, gum, tea, vitamin complexes, health functional supplements, food additives, etc. It can be used and can be used in the form of powder, granule, tablet, capsule or beverage. In addition to the above, it may be in any food form.

The food composition of the present invention may include about 40 to 80% by weight, preferably 50 to 80% by weight of the licorice and ginger complex extract based on the total weight of the composition for the purpose of preventing or improving atopic or asthma.

In addition, the food composition of the present invention may be prepared and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like for the purpose of preventing and / or improving atopic or asthma.

For example, the dietary supplement in the form of tablets is a mixture of licorice and ginger complex extracts, excipients, binders, disintegrants, and other additives in a conventional manner, and then compression molding with a lubricant or the like, The mixture can be compression molded directly.

In addition, the health functional food in the form of tablets may contain a mating agent and the like, if necessary, may be coated with a suitable coating agent.

Hard capsules of the health functional food in the form of capsules can be prepared by filling a conventional hard capsules with a mixture of additives such as licorice and ginger complex extracts, and excipients or granules or peeled granules thereof. And a mixture with a ginger complex extract and additives such as excipients may be prepared by filling a capsule base such as gelatin.

The soft capsule agent may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, as necessary.

Ring-shaped dietary supplements can be prepared by molding mixtures of licorice and ginger complex extracts, excipients, binders, disintegrants, etc. in a suitable manner. You can also be greeted with a suitable substance.

The health functional food in the form of granules may be prepared by granulating a mixture of licorice and ginger complex extracts, excipients, binders, disintegrants, etc. in a suitable manner, and may contain a flavoring agent, a mating agent and the like as necessary. For the health functional food in the form of granules, No. 12 (1680 μm), No. 14 (1410 μm) and No. 45 (350 μm) were used for the next particle size test. Less than 5.0% and passing through the 45 can be less than 15.0% of the total amount.

The term definitions of the excipients, binders, disintegrants, glidants, copulation agents, flavoring agents, etc. are described in the literature known in the art and include those having the same or similar functions (Korean Pharmacopoeia, Munseongsa, Korea Pharmacy University Council, 5th edition, p33-48, 1989).

The present invention provides a cosmetic composition for improving or preventing atopy consisting of the licorice and ginger complex extract as an active ingredient.

The cosmetic composition of the present invention may further include an additive or the like commonly used in cosmetic preparation in addition to the above-mentioned effective ingredient.

The cosmetic composition may be prepared in various forms according to a conventional cosmetic preparation method using a complex extract of licorice and ginger.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundation shampoos, hair lotions, hair creams, hair gels, sprays, and the like, but are not limited thereto.

Specific examples of these preparations include softening cream, astringent makeup, nourishing cream, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil , Body essences, makeup bases, foundations, hair dyes, shampoos, rinses and body cleansers.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to licorice and ginger complex extracts, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and the like. Cosmetically acceptable carriers. The type and amount of these components can be selected to those skilled in the art for understanding.

Acceptable carriers for cosmetics include, but are not limited to, purified water, oils, waxes, fatty acids, fatty alcohols, fatty acid esters, surfactants, humectants, thickeners, viscosity stabilizers, chelating agents, buffers, preservatives, lower alcohols, and the like. It is not. Whitening agents, moisturizers, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, and dyes may be included as needed.

The composition consisting of the licorice and ginger complex extract of the present invention is effective in the treatment or prevention of asthma and atopy compared to the extract of licorice and ginger respectively.

1 is a graph (A) and a graph (B) showing the concentration change of IFN-γ concentration according to the licorice and ginger complex extract treatment in splenocytes.
Figure 2 is a graph (A) and a graph (B) showing the concentration change of IFN-γ concentration according to the licorice and ginger single extract treatment in splenocytes.

Examples and preparation examples are provided to aid the understanding of the present invention. The following examples and preparations are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples and preparations.

Example

< Example  1> Licorice & Ginger Compound Water extract  Produce

Licorice and ginger were purchased through Omniherb (Daegu, Korea). 33.3 and 66.6 g of licorice and ginger were added to 500 mL of water, and the mixture was heated at 100 degrees Celsius for 2 hours, extracted with reflux cooling, filtered and lyophilized under reduced pressure to obtain a freeze-dried product.

Example 2 Preparation of Licorice and Ginger Complex Methanol Extract

Licorice and ginger were purchased through Omniherb (Daegu, Korea). After adding 33.3 and 66.6 g of licorice and ginger to 500 mL of methanol, respectively, the mixture was heated at 45 degrees Celsius for 6 hours, extracted with reflux cooling, filtered and lyophilized under reduced pressure to obtain a freeze-dried product.

< Example  3> Preparation of Licorice and Ginger Compound Ethanol Extract

A lyophilizate was prepared in the same manner as in Example 2, except that ethanol was used instead of methanol.

Comparative example

< Comparative example  1> Licorice Water extract  Produce

Licorice was purchased through Omniherb (Daegu, Korea). To 50 g of licorice, 500 mL of water was added, heated at 100 degrees Celsius for 2 hours, extracted with reflux cooling, filtered, and lyophilized under reduced pressure to obtain a freeze-dried product.

< Comparative example  2> ginger Water extract  Produce

Ginger was purchased through Omniherb (Daegu, Korea). 50 g of ginger was added to 500 mL of water, heated at 100 ° C. for 2 hours, extracted with reflux cooling, filtered, and lyophilized under reduced pressure to obtain a freeze-dried product.

Experimental Example

Experimental Example 1 Confirmation of Asthma and Atopy Treatment or Prevention Effect

Asthma and atopy show that Th 2 cells are activated to increase IL-4 secretion and IFN-γ secretion. Thus, asthma and atopy can be treated by measuring IFN-γ and IL-4 as follows. Or the prophylactic effect was confirmed.

Balb / C body weight 20-21 g (6 week old) mouse females were purchased from Central Animal Experiments Inc. and used for the experiments. All preparations were carried out in a sterile environment. Mice were euthanized after cervical dissection and spleens were removed and splenocytes were separated using two slide glasses prepared in advance. Centrifuge splenocytes at 1500 rpm for 5 minutes, discard supernatant, tap, add 0.75 ml of RBC Lysis Buffer (Sigma-Aldrich; St. Louis, MO, USA), and react for 1 minute, then RPMI 1640 culture solution (HyClone Laboratories, Inc.). Splenocytes were washed three times using Logan, UT, USA).

Splenocytes 2 × 10 ⁶ cells / ml isolated for IFN-γ and IL-4 measurements were collected in 24 well plates with 2 ug / of Concanavalin A (Con A) (Sigma-Aldrich; St. Louis, MO, USA). Stimulation with ml and the complex extract of licorice and ginger of Example 1 were treated with a concentration of 100 ug / ml, respectively, and then incubated for 48 hours. For the control group, phosphate-buffered saline (PBS) (USB Corporation; Cleveland, OH, USA) was treated in the same amount as the extract. After incubation, the supernatants were collected, and the concentrations of IFN-γ and IL-4 were measured using a sandwich cytosine kit using Mouse CytoSet Kit (Invitrogen, CA, USA). Each standard curve was drawn to calculate the concentration. The results are shown in FIG. Figure 1A is a graph showing the change in concentration of IFN-γ according to the treatment of licorice and ginger complex water extract, Figure 1B is a graph showing the change in concentration of IL-4 according to the treatment of licorice and ginger complex water extract (Con: control, 100: licorice and ginger composite water extract 100 ug / ml drug treatment group).

As shown in Figure 1, the complex water extract was found to increase IFN-γ by 107.47% and 61.92%, respectively, at 100 ug / ml concentration compared to the control group. Therefore, it can be seen that the extract increases IFN-γ and inhibits IL-4 in a concentration-dependent manner, thereby increasing Th 1 response and inhibiting Th 2 response. This means that the extract has an effect of treating or preventing asthma and atopy by controlling Th 1 and Th 2 responses.

Comparative Example 1

The same method as the above Experimental Example except that the licorice extract of Comparative Example 1 and the ginger extract of Comparative Example 2 were treated at concentrations of 33 ug / ml and 66 ug / ml, respectively, instead of the complex extract of licorice and ginger of Example 1 As a result, secretion levels of IFN-γ and IL-4 were measured.

The results are shown in Fig. Figure 2A is a graph showing the change in concentration of IFN-γ according to licorice and ginger water extract treatment, Figure 2B is a graph showing the change in concentration of IL-4 according to the treatment of licorice and ginger extract (Con: control, 33: Licorice water extract 33 ug / ml drug treatment group, 66: ginger water extract 66 ug / ml drug treatment group).

As shown in FIG. 2, the licorice extract showed a 66.82% increase in IFN-γ compared to the control at 33 ug / ml and 53.30% inhibition of IL-4 at 33 ug / ml compared to the control. Ginger water extract was found to reduce IFN-γ by 36.94% and 33.85% of IL-4 compared to the control at 33 ug / ml concentration.

As confirmed through the experimental example, the composite extract was confirmed that the IFN-γ increase rate and IL-4 inhibition rate increased about 2 times compared to the licorice and ginger single extract.

Formulation example

<Example 1> Pharmaceutical composition-licorice and ginger composite water extract

The capsule was prepared by filling 600 mg of licorice and ginger complex water extract of Example 1, 3 mg of crystalline cellulose, 14.8 mg of lactose, and 0.2 mg of magnesium stearate into gelatin capsules.

Preparation Example 2 Food Composition-Licorice and Ginger Complex Water Extract

Water was added to the licorice and ginger composite water extract of Example 1 (1% by weight), liquid fructose (0.5% by weight), oligosaccharides (2% by weight), sugar (2% by weight), and salt (0.5% by weight). After adjusting the remaining amount, the mixture was homogeneously mixed and instant sterilized to prepare a healthy beverage.

Claims (10)

A composition for the treatment or prevention of asthma or atopy consisting of licorice and ginger complex extract as an active ingredient. A pharmaceutical composition for the treatment or prevention of asthma or atopy consisting of a licorice and ginger complex extract as an active ingredient. The pharmaceutical composition of claim 2, wherein the ratio of licorice and ginger is 1: 1 to 1: 3. The pharmaceutical composition of claim 2, further comprising a pharmaceutically acceptable carrier, excipient or diluent. Food composition for the prevention or improvement of asthma or atopy consisting of licorice and ginger complex extract as an active ingredient. The food composition of claim 5, wherein the ratio of licorice and ginger is 1: 1 to 1: 3. 6. A food composition according to claim 5, further comprising a food acceptable carrier, excipient or diluent. Cosmetic composition for the prevention or improvement of asthma or atopy consisting of licorice and ginger complex extract as an active ingredient. The cosmetic composition according to claim 8, wherein the ratio of licorice and ginger is 1: 1 to 1: 3. The cosmetic composition according to claim 8, further comprising a cosmetically acceptable carrier.

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